CN118127072A - 番茄基因SlCBL11在增强番茄抗旱性中的应用 - Google Patents
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Abstract
本发明属于植物基因工程和分子育种技术领域，具体公开了番茄基因SlCBL11在增强番茄抗旱性中的应用，通过CRISPR/Cas9基因编辑技术敲除番茄基因SlCBL11，能够在后代中去除Cas9序列，避免了转基因技术引入外源基因的问题，不会影响番茄内其余基因的正常功能。本发明获得了敲除SlCBL11的纯合突变体，通过一系列实验证明：与野生型相比，SlCBL11基因敲除突变体获得了更高的抗旱性，且不干扰植株的生长发育，是一种理想的抗旱种质资源。
Description
技术领域
本发明涉及植物基因工程和分子育种技术领域，特别是一种番茄基因SlCBL11在增强番茄抗旱性中的应用。
背景技术
番茄(Solanum lycopersicum)具有丰富的营养成分，是世界范围内种植最广泛的大宗蔬菜之一，在番茄的整个生育过程中容易遭受许多非生物胁迫，导致产量和品质的下降。而干旱是影响番茄产量的十分重要的非生物胁迫之一，干旱会造成生育期的番茄大量落花落果，导致番茄的产量降低。番茄作为全世界消耗量最大的蔬菜作物之一，我国是世界上番茄种植面积最大，产量最多的国家，因此探究番茄抗旱的机理，培育具有抗旱性的新番茄品种，建立自有的抗旱体系十分重要。
目前，通过基因编辑的方法可以鉴定和筛选许多与番茄抗干旱下脱落的相关基因。然而很多抗旱基因的发掘的和研究集中在果实的品质和大小，对于落花的性状关注较少。所以现有的技术在改善番茄落花方面的抗旱性反面仍有不足。
发明内容
为了解决上述技术问题，本发明提供了一种番茄基因SlCBL11在增强番茄抗旱性中的应用。
为达到上述目的，本发明是按照以下技术方案实施的：
番茄基因SlCBL11在增强番茄抗旱性中的应用，所述番茄基因SlCBL11的核苷酸序列如SEQ ID NO.1所示。
具体地，通过敲除番茄基因SlCBL11，提高干旱条件下番茄植株的生长素含量，从而获得番茄花器官的脱落增强抗旱性的株系。
进一步地，敲除番茄基因SlCBL11的方法为：
1)设计特异靶向SlCBL11基因编码序列的sgRNA构建CRISPR/Cas9载体，进而合成一对sgRNA引物，将gRNA引物退火形成互补双链；sgRNA的序列如SEG ID NO.2所示；
2)酶切载体，进行连接，获得CRISPR/Cas9载体；
3)将CRISPR/Cas9载体转入大肠杆菌，获得CRISPR/Cas9载体的大肠杆菌；
4)通过番茄叶盘法将CRISPR/Cas9载体的大肠杆菌转化进入番茄中，获得T0代转基因材料通过对T0的番茄进行种植，杂交和筛选获得纯和的SlCBL11基因编辑植株即敲除番茄基因SlCBL11的突变体番茄植株。
进一步地，所述sgRNA引物包括：如SEG ID NO.3所示的上游引物和如SEG ID NO.4所示的下游引物。
与现有技术相比，本发明通过CRISPR/Cas9基因编辑技术敲除番茄基因SlCBL11，能够在后代中去除Cas9序列，避免了转基因技术引入外源基因的问题，不会影响番茄内其余基因的正常功能。本发明获得了敲除SlCBL11的纯合突变体，通过一系列实验证明：与野生型相比，SlCBL11基因敲除突变体获得了更高的抗旱性，且不干扰植株的生长发育，是一种理想的抗旱种质资源。
附图说明
图1为番茄SlCBL11基因敲除突变体的CRISPR/Cas9靶点位点测序分析，SlCBL11基因结构分析和CRISPR/Cas9靶点位置：▋代表编码序列；红色三角表示基因上靶点的位置；序列上的红色字体显示靶点序列；黑色框表示PAM；蓝色字表示编辑的位置。
图2为番茄SlCBL11基因敲除植株蛋白质序列。
图3为正常浇水和干旱处理下SlCBL11突变体和野生型的脱落率测定，对照为正常浇水；干旱为干旱处理。
图4为正常浇水和干旱处理下SlCBL11突变体和野生型的花中生长素含量，对照为正常浇水；干旱为干旱处理。
具体实施方式
为使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步的详细说明。此处所描述的具体实施例仅用于解释本发明，并不用于限定发明。
实例1：CRISPR/Cas9创制番茄SlCBL11敲除植株突变体
(1)CRISPR/Cas9载体的构建
1)根据SlCBL11基因的编码序列(SEQ ID NO.1)，利用sgRNA设计网站(http；//crispr.dbcls.jp/)设计Cas9编辑的靶点序列sgRNA：5’-CAAAGCACAATGGAATCTT-3’(SEQ IDNO.2)，依据靶点设计gRNA引物：
F:5’-CAAAGCACAATGGAATCTTGGG-3’(SEQ ID NO.3)；
R:5’-CCCAAGATTCCATTGTGCTTTG-3’(SEQ ID NO.4)。
将gRNA引物退火形成互补双链，具体反应体系和反应条件如表1、表2所示。
表1反应体系
表2反应条件
37℃ 30min
95℃ 5min
自然降温至室温
2)酶切载体，进行连接，酶切体系如3所示，连接体系如4所示。
表3酶切体系
表4连接体系
3)将5-10μl连接产物转化至大肠杆菌感受态(DH5α)，详情操作方法可见大肠杆菌的标准转化方法，转化菌液涂布至卡那霉素抗性培养基，37℃培养12小时，进行菌落PCR鉴定。鉴定引物如下：F 5’gtaaaacgacggccagt3’；R5’ccagaaattgaacgccgaag3’(SEQ IDNO.4)将鉴定结果正确的载体提取质粒、保存菌株，进行后续实验。
4)利用番茄叶盘法将CRISPR/Cas9载体转化值番茄(Ailsa Craig)获得转基因番茄植株。
5)基因编辑植株的鉴定
提取番茄基因组，对于基因编辑植株的基因组进行编辑方式的鉴定。以基因编辑植株的DNA为模板进行PCR扩增。鉴定引物如下：
F:5’-ACTATTTCTTGAACAGATGAAGTTG-3’(SEQ ID NO.5)
R:5’-ATACCATTAGGAGCAGGATTACACA-3’(SEQ ID NO.6)
将PCR产物进行测序。利用DNAman软件将测序结果与番茄基因组进行比对。鉴定出SlCBL11基因的一个突变纯和体(slCBL11-11)。突变体在靶点处增加了一个碱基(图1)，导致SlCBL11发生移码突变蛋白质提前终止(图2)，从而导致该基因的功能丧失。
实施例2：SlCBL11基因对番茄抗旱性的调控研究
为了检测SlCBL11植株的抗旱性，我们对其进行了自然干旱处理。将实施例1鉴定出的SlCBL11基因的突变体与对照品种Ailsa Craig种植在温室中，待番茄开花前天，将需要处理的植株浸泡在在水中12小时，后倒掉多余的水，进行7点的干旱处理。
如图3所示。正常浇水和干旱下，SlCBL11突变体和野生型的脱落情况著差异。正常浇水下，SlCBL11突变体和野生型的脱落率无变化。而干旱处理后SlCBL11突变体的脱落率显著低于野生型(图3)。以上结果说明SlCBL11负调控植株的抗旱性。
实施例3：slCBL11植株抗旱机理的研究
番茄花器官的脱落与生长素含量有关。我们选取干旱处理7天后的SlCBL11基因编辑植株和野生型的花，进行生长素含量的测定。结果如图4所示，在正常浇水下，SlCBL11突变体和野生型花中生长素含量无显著差异。在干旱条件下SlCBL11基因编辑植株含有较高的生长素含量(图4)。这说明干旱胁迫下SlCBL11可以通过调控生长素含量，进而调节番茄花器官的脱落增强抗旱性。
本发明的技术方案不限于上述具体实施例的限制，凡是根据本发明的技术方案做出的技术变形，均落入本发明的保护范围之内。
Claims (4)
1.番茄基因SlCBL11在增强番茄抗旱性中的应用，其特征在于：所述番茄基因SlCBL11的核苷酸序列如SEQ ID NO.1所示。
2.根据权利要求1所述的番茄基因SlCBL11在增强番茄抗旱性中的应用，其特征在于：通过敲除番茄基因SlCBL11，提高干旱条件下番茄植株的生长素含量，从而获得番茄花器官的脱落增强抗旱性的株系。
3.根据权利要求2所述的番茄基因SlCBL11在增强番茄抗旱性中的应用，其特征在于，敲除番茄基因SlCBL11的方法为：
1)设计特异靶向SlCBL11基因编码序列的sgRNA构建CRISPR/Cas9载体，进而合成一对sgRNA引物，将gRNA引物退火形成互补双链；sgRNA的序列如SEGID NO.2所示；
2)酶切载体，进行连接，获得CRISPR/Cas9载体；
3)将CRISPR/Cas9载体转入大肠杆菌，获得CRISPR/Cas9载体的大肠杆菌；
4)通过番茄叶盘法将CRISPR/Cas9载体的大肠杆菌转化进入番茄中，获得T0代转基因材料通过对T0的番茄进行种植，杂交和筛选获得纯和的SlCBL11基因编辑植株即敲除番茄基因SlCBL11的突变体番茄植株。
4.根据权利要求3所述的番茄基因SlCBL11在增强番茄抗旱性中的应用，其特征在于：所述sgRNA引物包括：如SEG ID NO.3所示的上游引物和如SEG ID NO.4所示的下游引物。
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