CN118105436A - Celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia and preparation method thereof - Google Patents

Abstract

The invention provides a feather cockscomb seed and concha haliotidis traditional Chinese medicine composition for treating myopia and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine compositions. The traditional Chinese medicine composition comprises feather cockscomb seed, rhizoma alismatis, concha haliotidis, chinese yam, poria cocos, dogwood, semen cassiae, chrysanthemum, cortex moutan, liquorice, angelica sinensis, polygala tenuifolia, butterflybush flower, dangshen, astragalus mongholicus, rehmannia glutinosa, medlar and mint. The traditional Chinese medicine composition provided by the invention has the advantages of nourishing kidney, tonifying yin, strengthening vital qi to eliminate pathogenic factor, treating both principal and secondary aspect of disease, improving microcirculation in eyes, adjusting diopter, increasing nutrition of eye muscles and the like.

Description

Celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia and preparation method thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia and a preparation method thereof.

Background

In recent years, with the development of social economy, the progress of science and technology and the change of life style, the incidence of myopia has been increasing year by year. Myopia not only affects physical, psychological, cognitive, social functions and quality of life, increasing socioeconomic burden, but also is associated with the development of a range of serious pathological conditions such as macular degeneration, retinal detachment, glaucoma, cataracts, vitreous opacity, retinal detachment and cleavage, etc. Deeply recognizes the factors causing myopia and makes a prevention and control policy and strategy, which is an important guarantee for promoting vision health.

Disclosure of Invention

In view of the above, the invention provides a feather cockscomb seed and concha haliotidis traditional Chinese medicine composition for treating myopia and a preparation method thereof.

In order to achieve the above purpose, the invention adopts the following technical scheme:

The invention provides a celosia-concha haliotidis traditional Chinese medicine composition for treating myopia, which comprises the following raw materials in parts by weight: 50-100 parts of celosia seed, 50-100 parts of rhizoma alismatis, 20-80 parts of concha haliotidis, 20-50 parts of Chinese yam, 20-50 parts of poria cocos, 20-50 parts of dogwood, 20-50 parts of semen cassiae, 20-50 parts of chrysanthemum, 20-50 parts of cortex moutan, 50-70 parts of liquorice, 10-40 parts of angelica sinensis, 10-30 parts of polygala tenuifolia, 10-30 parts of buddleja officinalis, 10-30 parts of radix codonopsis pilosulae, 10-30 parts of astragalus mongholicus, 10-30 parts of radix rehmanniae preparata, 5-30 parts of medlar and 10-20 parts of mint.

Further, the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia comprises the following raw materials in parts by weight: 80 parts of feather cockscomb seed, 80 parts of rhizoma alismatis, 70 parts of concha haliotidis, 30 parts of Chinese yam, 30 parts of poria cocos, 30 parts of dogwood, 30 parts of semen cassiae, 20 parts of chrysanthemum, 30 parts of cortex moutan, 60 parts of liquorice, 20 parts of Chinese angelica, 20 parts of polygala tenuifolia, 20 parts of butterflybush flower, 20 parts of codonopsis pilosula, 20 parts of astragalus mongholicus, 20 parts of radix rehmanniae preparata, 10 parts of wolfberry fruit and 10 parts of mint.

The second aim of the invention is to provide a preparation method of the celosia-concha haliotidis traditional Chinese medicine composition for treating myopia, which comprises the following steps:

(1) Weighing the raw materials according to the parts by weight, mixing, crushing and sieving to obtain a mixture for standby;

(2) Adding water into the mixture obtained in the step (1), uniformly mixing, and sequentially extracting, centrifuging and filtering to obtain an extracting solution for later use;

(3) And (3) adding a complex enzyme preparation into the extracting solution in the step (2) for enzymolysis, inactivating enzyme after the enzymolysis is finished, cooling to room temperature, centrifuging, and taking supernatant for freeze drying to obtain the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia.

Further, the crushed materials in the step (1) are sieved by a sieve of 18-35 meshes, and more preferably a sieve of 20 meshes.

Further, the water in the step (2) is added in a proportion of 10-30 times of the total mass of the mixture.

Further, in the step (2), ultrasonic extraction is adopted, the extraction time is 0.5-2h, the extraction temperature is 40-60 ℃, and the extraction power is 200-800W.

Further, the centrifugal rotating speed in the step (2) and the centrifugal rotating speed in the step (3) are 3600-8000r/min, and the centrifugal time is 5-25min.

Further, the compound enzyme preparation in the step (3) has a mass ratio of 1-3:3-4:2-5, pentose converting enzyme and peroxidase.

Further, the enzyme deactivation mode in the step (3) is boiling, and the enzyme deactivation time is 10-25min.

Further, the enzymolysis temperature in the step (3) is 15-55 ℃, and more preferably 30-37 ℃.

The seed of celosia is derived from dry mature seeds of celosia (celosiaargenteaal) belonging to the genus celosia of the family amaranthaceae, and is originally carried in Shennong Ben Cao Jing, and is a pharmacopoeia harvest-carrying variety. It is bitter in flavor, slightly cold in nature and enters liver meridian. Has effects of removing liver fire, improving eyesight, and removing nebula. Is used for treating conjunctival congestion due to liver heat, nebula, dim eyesight, dizziness due to liver fire, and dizziness.

Alismatis rhizoma has sweet, light and cold property. It has effects of promoting diuresis, removing dampness, clearing heat, eliminating turbid pathogen and reducing blood lipid.

Concha Haliotidis has effects of suppressing hyperactive liver, subsiding yang, removing heat, and improving eyesight. It has therapeutic effects on wind-yang disturbance, headache, dizziness, convulsion, bone steaming, fatigue, and blind.

Chinese yam is sweet in taste, cool and moist in nature, enters lung, spleen and kidney meridians, and has the effects of invigorating spleen, tonifying lung, reinforcing kidney and replenishing essence. For diarrhea due to spleen deficiency, chronic dysentery, cough due to consumptive disease, diabetes, spermatorrhea, leukorrhagia, frequent urination. Spleen and stomach tonifying, body fluid generating, lung nourishing, kidney tonifying and seminal emission astringing. Can be used for treating spleen deficiency, anorexia, chronic diarrhea, asthma and cough due to lung deficiency, spermatorrhea due to kidney deficiency, leukorrhagia, frequent urination, and diabetes due to deficiency heat. Bran-fried yam can invigorate spleen and stomach. It is used for treating spleen deficiency, anorexia, diarrhea, and loose stool.

Poria, it is sweet and light in taste, neutral in nature, and enters heart, lung, spleen and kidney meridians. Has effects in promoting diuresis, removing dampness, invigorating spleen, and tranquilizing mind.

Fructus Corni is a astringent, and is dried mature pulp of fructus Corni of the family Leucocarpaceae. Sour and astringent properties and slightly warm. It enters liver and kidney meridians. Cornus officinalis is sour and astringent, sweet and slightly warm, and enters liver and meridian. It is good at warming, tonifying liver and kidney, astringing and inducing astringency. Or Yun, which is a product of tonifying both yin and yang, and tonifying kidney essence. It is indicated for deficiency of liver and kidney, deficiency of sweat, metrorrhagia and metrostaxis.

Semen Cassiae, semen Caprae Seu Ovis, cornu Naemorhedi, herba Zosterae Marinae, semen Myristicae, ma Tizi, semen Apii Graveolentis, semen Caprae Seu Ovis, and semen Phaseoli Radiati. Bitter and sweet in taste and cool in nature, has the effects of clearing liver fire, dispelling wind-damp, tonifying kidney, improving eyesight, relaxing bowel and the like.

Flos Chrysanthemi is a dry head like inflorescence of Chrysanthemum ChrysanthemummorifoliumRamat belonging to Compositae, and has pungent, sweet and bitter taste and slight cold. It enters lung and liver meridians. The chrysanthemum has the main effects of dispelling wind-heat, stabilizing liver yang, clearing liver and improving vision, clearing heat and detoxicating, and is used for treating wind-heat type common cold, initial warm diseases, liver yang hyperactivity, headache dizziness, conjunctival congestion and swelling and pain, eye dizziness, sore and carbuncle swelling and toxicity.

Cortex moutan is dry root bark of Paeonia suffruticosa of Polygonaceae, and has bitter and pungent properties and slight cold property. It enters heart, liver and kidney meridians. Cortex moutan is bitter and pungent in flavor and can clear away slight cold energy, clear away and promote the circulation of the powder. Enters heart and liver meridians, and is good at clearing heat and cooling blood, promoting blood circulation and removing blood stasis; enter kidney meridian and can dispel deficiency heat. It is indicated for single or concurrent cases with blood heat with blood stasis or blood stasis with heat or deficiency heat with blood stasis or without sweat and bone steaming because it integrates the functions of clearing blood heat, removing deficiency heat and dispelling blood stasis.

Glycyrrhrizae radix is dry root and rhizome of Glycyrrhiza uralensis Fisch, glycyrrhiza uralensis Fisch or Glycyrrhiza glabra of Leguminosae, and has good properties, and can be used for heart, lung, spleen and stomach channels. Licorice root, radix Glycyrrhizae Praeparata, sweet in taste, nourishes and moistens slowly, produces flat and cool, and is roasted to calm and warm, and enters spleen and lung meridians, stomach and heart meridians. Not only tonify qi and strengthen middle energizer, but also relieve spasm and pain, alleviate drug properties, and also eliminate phlegm and relieve cough and detoxify. The roasted qi-tonifying and urgent-relieving force is strong; it can purge fire and detoxify.

Dang Gui is the dry root of Dang Gui of Umbelliferae, sweet and pungent in flavor, warm in nature, enters liver, heart and spleen meridians, sweet in flavor and tonifying and moist, pungent in flavor and warm in property, enters liver and heart meridians and spleen meridians. It is good at replenishing blood and activating blood, regulating menstruation and relieving pain, relaxing bowel and expelling cold, and is suitable for treating constipation due to blood deficiency and blood stasis, especially constipation due to dryness of the intestines, and is not only a key herb for regulating menstruation in gynecology but also a good blood-replenishing product in internal medicine and is commonly used for detumescence and wound therapy in surgery.

Radix Polygalae is the dry root of Polygala tenuifolia Willd or Polygala tenuifolia Willd of Polygalaceae, and has bitter and pungent properties and is warm. It enters heart, kidney and lung meridians. Polygala tenuifolia Xin Sangu is used to relieve heat and to treat heart, lung and kidney channels. Not only can invigorate heart yang and nourish heart qi, but also can induce resuscitation by dispelling phlegm, so as to treat heart-mind restlessness or phlegm obstructing heart orifices. It also has the actions of resolving phlegm, relieving cough, resolving carbuncle, swelling and pain, and is indicated for cough with excessive phlegm, sore and abscess.

Buddleja officinalis is dried flower bud and inflorescence of Buddleja officinalis of Strychnaceae, and has sweet medicinal properties and slight cold. Enter liver meridian. Sweet and slightly cold, clear and purge and tonify, enter liver and gallbladder meridians. Is effective in eliminating pathogenic factors and strengthening body resistance. It is indicated for both liver-fire and liver-blood deficiency with heat because it is a key herb for eye diseases.

Dried root of Codonopsis pilosula, or Codonopsis pilosula, of Campanulaceae, has sweet and flat properties. Enter spleen and lung meridians. The dangshen is sweet and tonic, but is not dry and greasy, and enters spleen and lung channels. Qi tonifying is inferior to ren Shen, and is usually indicated for mild cases of qi deficiency of spleen and lung. It is also indicated for fluid deficiency and blood deficiency because it can promote fluid production and nourish blood.

Astragalus membranaceus is the dried root of Astragalus mongholicus bge or Astragalus membranaceus bge of Leguminosae, has sweet medicinal properties and slightly warmth, and enters spleen and lung channels. Gan Wenbu liters of astragalus root, sweet and bland, and excreted; it is used with mild condition and stir-baked with honey to warm the spleen and lung meridians to strengthen the body resistance and remove pathogenic factors. It is good at tonifying middle-jiao and Qi, promoting the production of qi and clearing yang, tonifying lung and Qi, invigorating stomach and consolidating exterior, treating spleen and lung Qi deficiency, sinking middle-jiao, qi failing to control blood, spontaneous perspiration and night sweat, etc., and also has the actions of supporting sore-toxin, inducing diuresis and relieving swelling, treating sore and carbuncle due to deficiency of qi and blood, or chronic ulcer and difficult healing, and qi deficiency and edema and dysuria.

Prepared rehmannia root is a processed product of dried rehmannia root, and has sweet and slightly warm property. Enter liver meridian. The chemical components of the composition are similar to that of radix rehmanniae, and mainly contain styrenesside components, acteoside and the like, as well as monosaccharides, amino acids, vitamin A substances and the like. Has effects of replenishing blood, nourishing yin, replenishing vital essence, and replenishing marrow.

Fructus Lycii is dry mature fruit of Lycium barbarum belonging to Solanaceae, has sweet property, and can treat liver and kidney meridian. The wolfberry fruit is sweet and tonic, and is calm and warm, and enters liver, kidney and lung meridians. It is good at nourishing liver and kidney to improve eyesight, and can be used for treating deficiency of liver-yin and kidney-yin and hypopsia; it can nourish lung yin to relieve cough, and treat cough due to yin deficiency.

Peppermint, a dry aerial part of the plant Mentha arvensis of Labiatae, is pungent in property and cool in nature, and enters the lung and liver meridians. Peppermint, herba Menthae, pungent and sparse, fragrant and can be used for removing dirt, cooling and clearing heat. Enters the lung and liver channels, and not only disperses wind and heat to clear head, eyes, throat and eruption, but also soothes liver and relieve depression and remove dirt. Stronger force is exerted, and good effect is achieved in clearing the head and eyes. It is indicated for wind-heat attacking the exterior or attacking the upper body, and can be used for treating liver depression transforming into heat.

Compared with the prior art, the invention has the following beneficial effects: the traditional Chinese medicine composition provided by the invention follows the compatibility principle of monarch, minister, assistant and guide when being proportioned, and aims at the cause of myopia on the basis of diagnosis and treatment, the characteristics of nourishing kidney and tonifying yin, strengthening vital qi and eliminating evil, treating both principal and secondary aspects of disease and difficult repetition are achieved by taking the basis of the nature and the taste, the efficacy, the channel tropism and the application of Chinese herbal medicines and utilizing the synergistic effect among the Chinese herbal medicines, and the compatibility of medicines used in the composition compound is ensured to be closely related to the symptoms.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the invention, are within the scope of the invention.

The test methods or test methods described in the following examples are all conventional methods unless otherwise specified; the starting materials and auxiliaries, unless otherwise specified, are obtained commercially from conventional sources or are prepared in conventional manner.

Example 1

A preparation method of a Chinese medicinal composition of feather cockscomb seed and concha haliotidis for treating myopia comprises the following steps:

(1) Weighing 50 parts of celosia seed, 50 parts of rhizoma alismatis, 30 parts of concha haliotidis, 20 parts of Chinese yam, 20 parts of poria cocos, 20 parts of dogwood, 20 parts of semen cassiae, 20 parts of chrysanthemum, 20 parts of cortex moutan, 50 parts of liquorice, 10 parts of Chinese angelica, 10 parts of polygala tenuifolia, 10 parts of buddleia officinalis, 10 parts of codonopsis pilosula, 10 parts of astragalus mongholicus, 10 parts of rehmannia glutinosa, 5 parts of medlar and 10 parts of mint, mixing, crushing, and sieving with a 18-mesh sieve to obtain a mixture for standby;

(2) Adding 10 times of water into the mixture obtained in the step (1), uniformly mixing, performing ultrasonic extraction for 0.5h at 40 ℃ with 200W of extraction power, centrifuging at 3600r/min for 10min after extraction, and collecting the extract for later use;

(3) Adding the extract in the step (2) with the mass ratio of 1:3:2, regulating the temperature of the system to 25 ℃ for enzymolysis for 10min, transferring to waste water for enzyme deactivation treatment after the enzymolysis is finished, cooling to room temperature, centrifuging, taking supernatant, and freeze-drying to obtain the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia.

Example 2

A preparation method of a Chinese medicinal composition of feather cockscomb seed and concha haliotidis for treating myopia comprises the following steps:

(1) Weighing the raw materials of 80 parts of feather cockscomb seed, 80 parts of rhizoma alismatis, 70 parts of concha haliotidis, 30 parts of Chinese yam, 30 parts of poria cocos, 30 parts of dogwood, 30 parts of cassia seed, 20 parts of chrysanthemum, 30 parts of tree peony bark, 60 parts of liquorice, 20 parts of Chinese angelica, 20 parts of polygala tenuifolia, 20 parts of butterflybush flower, 20 parts of codonopsis pilosula, 20 parts of astragalus mongholicus, 20 parts of rehmannia glutinosa, 10 parts of medlar and 10 parts of mint, mixing, crushing, and sieving with a 20-mesh sieve to obtain a mixture for standby;

(2) Adding 20 times of water into the mixture obtained in the step (1), uniformly mixing, performing ultrasonic extraction for 1h at 50 ℃ with 600W of extraction power, centrifuging in a centrifuge at 3600r/min for 20min after extraction, and collecting extract for later use;

(3) Adding the extract in the step (2) with the mass ratio of 2:3:4, regulating the temperature of the system to be 35 ℃ for enzymolysis for 15min, transferring the enzymolysis product to waste water for enzyme deactivation treatment after the enzymolysis is finished, cooling the product to room temperature, centrifuging the product, and taking supernatant for freeze drying to obtain the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia.

Example 3

A preparation method of a Chinese medicinal composition of feather cockscomb seed and concha haliotidis for treating myopia comprises the following steps:

(1) Weighing 100 parts of celosia seed, 100 parts of rhizoma alismatis, 80 parts of concha haliotidis, 50 parts of Chinese yam, 50 parts of poria cocos, 50 parts of dogwood, 50 parts of semen cassiae, 50 parts of chrysanthemum, 50 parts of moutan bark, 70 parts of liquorice, 40 parts of Chinese angelica, 30 parts of polygala tenuifolia, 30 parts of butterflybush flower, 30 parts of codonopsis pilosula, 30 parts of astragalus mongholicus, 30 parts of rehmannia glutinosa, 20 parts of medlar and 20 parts of mint, mixing, crushing, and sieving with a 35-mesh sieve to obtain a mixture for standby;

(2) Adding 30 times of water into the mixture obtained in the step (1), uniformly mixing, performing ultrasonic extraction for 2 hours at 60 ℃ with 800W of extraction power, centrifuging in a centrifuge at 8000r/min for 25min after extraction, and collecting extract for later use;

(3) Adding the extract in the step (2) into the extract with the mass ratio of 3:4:5, regulating the temperature of the system to 45 ℃ for enzymolysis for 30min, transferring the enzymolysis product into waste water for enzyme deactivation treatment after the enzymolysis is finished, cooling the waste water to room temperature, centrifuging the waste water, and taking supernatant for freeze drying to obtain the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia.

Efficacy verification is carried out on the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia, which is prepared in the embodiment 1-3.

The construction method of experimental materials and myopia animal models comprises the following steps:

Selecting 100 chickens hatched for 2 days as experimental objects, and visually depriving the left eyes of the chickens for 15 days by adopting a semitransparent film eyeshade covering method, and marking the chickens as a visual deprivation group; the right eye of the chickens served as a control group. In the process of model construction, natural circadian rhythm, conventional pellet feed feeding and free drinking water and eating.

The vision deprivation group and the control group are sequentially subjected to image checking and eye A hyper-measurement of the length of the eye axis. The specific operation is as follows: after a specified time period, the experimental animals were sacrificed, the eyeballs were removed, sagittal longitudinal cuts were made in the middle area of the eyeballs, and scleral cartilage layer and fibrous layer thicknesses were measured using metamorphi image analysis software.

2. Construction results:

Animals in the vision deprivation group developed high myopia (-14.1+ -0.5D), increased eye axis by 10.32+ -0.25 mm, diopter of the right eye (+1.1+ -0.3D), eye axis by 7.41+ -0.17 mm, and significant differences (P < 0.05) compared to diopter of the right eye (+1.1+ -0.3D), eye axis by 7.41+ -0.17 mm, i.e., successful in constructing a myopic animal model.

3. Drug administration result detection

And randomly selecting 40 myopic chickens in a successfully constructed myopia animal model, and randomly dividing the model into four groups I, II, III and IV, wherein the groups I, II and III respectively correspond to the traditional Chinese medicine compositions prepared in the examples 1,2 and 3, the dosage of the traditional Chinese medicine composition is 2.1rnl/100g of body weight according to the equivalent dosage conversion of human and animals, the traditional Chinese medicine particles are dissolved in water and then are administrated by stomach irrigation once daily, each time is 2.1 ml, the administration is continued for 5 weeks, and the diopter of the left eye of the chickens is measured once every week. Group IV were irrigated with an equal volume of purified water daily.

The results are shown in Table 1

Table 1 results of diopter measurements in chickens

As is clear from the above table, the traditional Chinese medicine composition prepared in examples 1-3 has obvious therapeutic effect on myopic chickens, and diopter is still kept in a normal range after stopping the medicine, but the diopter of group IV is not improved obviously. The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (10)

1. The celosia seed-concha haliotidis traditional Chinese medicine composition for treating myopia is characterized by comprising the following raw materials in parts by weight: 50-100 parts of celosia seed, 50-100 parts of rhizoma alismatis, 20-80 parts of concha haliotidis, 20-50 parts of Chinese yam, 20-50 parts of poria cocos, 20-50 parts of dogwood, 20-50 parts of semen cassiae, 20-50 parts of chrysanthemum, 20-50 parts of cortex moutan, 50-70 parts of liquorice, 10-40 parts of angelica sinensis, 10-30 parts of polygala tenuifolia, 10-30 parts of buddleja officinalis, 10-30 parts of radix codonopsis pilosulae, 10-30 parts of astragalus mongholicus, 10-30 parts of radix rehmanniae preparata, 5-30 parts of medlar and 10-20 parts of mint.

2. The Chinese medicinal composition for treating myopia, which is prepared from the following raw materials in parts by weight: 80 parts of feather cockscomb seed, 80 parts of rhizoma alismatis, 70 parts of concha haliotidis, 30 parts of Chinese yam, 30 parts of poria cocos, 30 parts of dogwood, 30 parts of semen cassiae, 20 parts of chrysanthemum, 30 parts of cortex moutan, 60 parts of liquorice, 20 parts of Chinese angelica, 20 parts of polygala tenuifolia, 20 parts of butterflybush flower, 20 parts of codonopsis pilosula, 20 parts of astragalus mongholicus, 20 parts of radix rehmanniae preparata, 10 parts of wolfberry fruit and 10 parts of mint.

3. The preparation method of the celosia-concha haliotidis traditional Chinese medicine composition for treating myopia according to any one of claims 1-2, which is characterized by comprising the following steps:

(1) Weighing the raw materials according to the parts by weight, mixing, crushing and sieving to obtain a mixture for standby;

(2) Adding water into the mixture obtained in the step (1), uniformly mixing, and sequentially extracting, centrifuging and filtering to obtain an extracting solution for later use;

(3) And (3) adding a complex enzyme preparation into the extracting solution in the step (2) for enzymolysis, inactivating enzyme after the enzymolysis is finished, cooling to room temperature, centrifuging, and taking supernatant for freeze drying to obtain the celosia seed and concha haliotidis traditional Chinese medicine composition for treating myopia.

4. The method for preparing a Chinese medicinal composition of celosia seed and sea-ear shell for treating myopia according to claim 3, wherein the crushed materials in the step (1) are sieved by a 18-35 mesh sieve.

5. The method for preparing a Chinese medicinal composition of celosia seed and sea-ear shell for treating myopia according to claim 3, wherein the water in the step (2) is added in a proportion of 10-30 times of the total mass of the mixture.

6. The method for preparing a Chinese medicinal composition of feather cockscomb seed and sea-ear shell for treating myopia according to claim 3, wherein the ultrasonic extraction is adopted in the step (2), the extraction time is 0.5-2h, the extraction temperature is 40-60 ℃, and the extraction power is 200-800W.

7. The method for preparing a Chinese medicinal composition of feather cockscomb seed and sea-ear shell for treating myopia according to claim 3, wherein the centrifugal rotational speed in the step (2) and the centrifugal rotational speed in the step (3) are 3600-8000r/min, and the centrifugal time is 5-25min.

8. The method for preparing the celosia-concha haliotidis traditional Chinese medicine composition for treating myopia according to claim 3, wherein the compound enzyme preparation in the step (3) is characterized by comprising the following components in percentage by mass: 3-4:2-5, pentose converting enzyme and peroxidase.

9. The method for preparing the celosia-concha haliotidis traditional Chinese medicine composition for treating myopia according to claim 3, wherein the enzyme deactivation mode in the step (3) is boiling, and the enzyme deactivation time is 10-25min.

10. The method for preparing a Chinese medicinal composition of celosia seed and sea-ear shell for treating myopia according to claim 3, wherein the enzymolysis temperature in the step (3) is 15-55 ℃.