CN117957305A - 细胞剥离剂和细胞分离方法 - Google Patents

细胞剥离剂和细胞分离方法 Download PDF

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Publication number
CN117957305A
CN117957305A CN202280047033.4A CN202280047033A CN117957305A CN 117957305 A CN117957305 A CN 117957305A CN 202280047033 A CN202280047033 A CN 202280047033A CN 117957305 A CN117957305 A CN 117957305A
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CN
China
Prior art keywords
cell
group
polymer
ureido group
cells
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Pending
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CN202280047033.4A
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English (en)
Chinese (zh)
Inventor
今村龙太郎
大岳知之
丸山厚
嶋田直彦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NOF Corp
Tokyo Institute of Technology NUC
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NOF Corp
Tokyo Institute of Technology NUC
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Publication date
Application filed by NOF Corp, Tokyo Institute of Technology NUC filed Critical NOF Corp
Publication of CN117957305A publication Critical patent/CN117957305A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
CN202280047033.4A 2021-06-30 2022-06-27 细胞剥离剂和细胞分离方法 Pending CN117957305A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-109278 2021-06-30
JP2021109278 2021-06-30
PCT/JP2022/025542 WO2023276937A1 (ja) 2021-06-30 2022-06-27 細胞剥離剤および細胞分離方法

Publications (1)

Publication Number Publication Date
CN117957305A true CN117957305A (zh) 2024-04-30

Family

ID=84691411

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280047033.4A Pending CN117957305A (zh) 2021-06-30 2022-06-27 细胞剥离剂和细胞分离方法

Country Status (5)

Country Link
US (1) US20240200025A1 (https=)
EP (1) EP4365279A4 (https=)
JP (1) JPWO2023276937A1 (https=)
CN (1) CN117957305A (https=)
WO (1) WO2023276937A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7761896B2 (ja) * 2021-06-30 2025-10-29 日油株式会社 細胞分離用基材および細胞分離方法
JPWO2024190766A1 (https=) * 2023-03-16 2024-09-19

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3441496B2 (ja) * 1993-11-17 2003-09-02 株式会社セルシード アフィニティー分離材料
CA2259559C (en) 1996-07-10 2004-11-09 E.I. Du Pont De Nemours And Company Polymerization with living characteristics
FR2764892B1 (fr) 1997-06-23 2000-03-03 Rhodia Chimie Sa Procede de synthese de polymeres a blocs
BR9815179A (pt) 1997-12-18 2000-10-10 Du Pont Processo para produzir um polìmero, polìmero, composição de revestimento e agente de transferência de cadeia.
US8822550B2 (en) * 2010-03-23 2014-09-02 Kyushu University, National University Corporation Temperature-, pH- or salt concentration-sensitive separation material and use thereof
JP2015174962A (ja) * 2014-03-17 2015-10-05 国立大学法人東京工業大学 ポリ（２−メタクリロイルオキシエチルホスホリルコリン−ｂ−２−ウレイドエチルメタクリレート）共重合体及びそれにより形成される高分子ミセル
JP2021109278A (ja) 2020-01-10 2021-08-02 株式会社ディスコ 加工装置

Also Published As

Publication number Publication date
EP4365279A1 (en) 2024-05-08
EP4365279A4 (en) 2025-06-25
WO2023276937A1 (ja) 2023-01-05
US20240200025A1 (en) 2024-06-20
JPWO2023276937A1 (https=) 2023-01-05

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