CN117919141A

CN117919141A - Composition with moisturizing and repairing functions and application thereof

Info

Publication number: CN117919141A
Application number: CN202410073338.3A
Authority: CN
Inventors: 黄俊豪
Original assignee: Guangdong Quanli Pharmaceutical Technology Co ltd
Current assignee: Guangdong Quanli Pharmaceutical Technology Co ltd
Priority date: 2024-01-18
Filing date: 2024-01-18
Publication date: 2024-04-26

Abstract

The invention provides a composition with moisturizing and repairing functions and application thereof, and relates to the technical field of daily chemicals; furthermore, the product of the invention can be used for repairing skin injury caused by various reasons, and has the functions of anti-inflammatory and moisturizing. The product can be used in cosmetics and skin care products, such as emulsion, cream, essence, etc., and is mild to human body, safe and non-irritating.

Description

Composition with moisturizing and repairing functions and application thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a moisturizing and repairing composition and application thereof in cosmetics.

Background

With the increasing demand of people for skin care, skin care becomes an important function of cosmetics. Moisturizing and repairing are a vital step in the skin care process, and particularly in modern life, the skin is extremely easy to dry, lack water, be sensitive and other problems due to various reasons such as environmental factors, bad living habits and the like. In order to solve these problems, moisturizing repair is receiving a great deal of attention.

The main purpose of moisturizing and repairing is to provide enough moisture and nutrient for skin, keep the water and oil balance of the skin, and make the skin in a healthy, smooth and elastic state. Meanwhile, the moisturizing repair is also beneficial to enhancing the barrier function of the skin, preventing invasion of external irritants and reducing skin injury and anaphylactic reaction.

In the skin care product market, moisturizing repair products are various, including moisturizing creams, moisturizing emulsions, masks and the like. These products generally contain moisturizing ingredients such as hyaluronic acid, glycerol, polysaccharides, and the like, which are effective in retaining moisture and enhancing the moisturizing ability of the skin. Or the water loss on the surface of the skin is slowed down by forming a sealing film on the surface of the skin by using components of high molecular polymers, but the problem of skin moisturizing and repairing cannot be fundamentally solved, and compared with the traditional product, a product prepared by taking plant extracts as active components has higher safety, and natural active components are easier to be absorbed by the skin, so that the effect of the product is more remarkable; the functionality is more prominent. Thus being popular with consumers. Therefore, research and development of natural plant active ingredients in applied cosmetics have become an important link for sustainable development of the cosmetic industry.

Disclosure of Invention

The invention aims to provide a moisturizing repair composition and application thereof, wherein the composition comprises horse chestnut extract, witch hazel extract, hedyotis diffusa extract and celery extract, and has excellent synergistic moisturizing repair effect.

In order to achieve the above object, the present invention provides a moisturizing repair composition comprising horse chestnut extract, witch hazel extract and hedyotis diffusa extract and celery extract.

Horse chestnut (Aesculus chinensis Bunge), a deciduous arbor plant of the genus horse chestnut of the family Sapindaceae, native to the chinese yellow river basin, contains abundant horse chestnut saponins (Escin) la, lb, IVc, IVd, IVe, IVh in its seeds; aesculin (Isoescin) Ia, ib and aesculin (Aesculuside) a are widely used in cosmetics and medicines. Has obvious anti-inflammatory, anticancer and vascular permeability enhancing effects.

Hamamelis (HAMAMELIS MOLLIS OLIV), a plant of Hamamelis genus of Hamamelidaceae family, is native to the Yangtze river basin of China. The leaves of the plant are rich in stone titanic acid, gallnut, caffeic acid and flavonoid derivatives, and are widely applied to diminishing inflammation and resisting bacteria; the bark contains rich active substances such as flavonol, gallnut, tannic acid and the like, and has obvious astringing effect on skin. Therefore, the whitening and repairing agent is widely applied to whitening and repairing products.

Herba Hedyotidis Diffusae (Ophiorrhiza mungos L), plant of Hedyotidis Diffusae of Rubiaceae, is widely distributed in Yangtze river basin. All the herbs are pharmaceutically acceptable. Contains abundant flavonoid compounds, triterpenoid saponins and other active ingredients, has obvious functions of resisting oxidation, inflammation, bacteria and the like, and is widely applied to whitening and moisturizing products.

Celery (Apium graveolens L.) is widely distributed in the whole country and is one of the medicinal and edible plants, and the leaves and seeds of the celery (Apium graveolens L.) contain rich flavonoid active substances such as apigenin, chalcone and the like, and the celery has the effects of resisting oxidation, inflammation, bacteriostasis and the like and is widely applied to foods or medicines.

The invention creatively compounds the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract into the moisturizing and repairing composition. Experiments prove that the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract interact with each other to play a synergistic effect, so that the moisturizing and repairing effects of the moisturizing and repairing composition are better.

Preferably, the raw materials of the composition comprise the following components in percentage by mass: 10-15 parts of horse chestnut extract, 5-15 parts of witch hazel extract, 5-7 parts of ophiopogon root extract and 25-30 parts of celery leaf extract, wherein the parts ratio of the horse chestnut extract, the witch hazel extract, the ophiopogon root extract and the celery extract is 1:0.5-1.2:0.1-0.5:2.5-3. Experiments show that when the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract are compounded according to the mass part ratio, the moisturizing and repairing composition has good moisturizing and repairing effects.

More preferably, the parts ratio of the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract is 1:0.7-1:0.2-0.4:2.6-2.8 according to the mass fraction ratio. Experiments show that when the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract are compounded according to the mass part ratio, the moisturizing and repairing composition has better moisturizing and repairing effects.

Most preferably, the parts ratio of the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract is 1:0.8:0.3:2.7 in terms of mass fraction. Experiments show that when the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract are compounded according to the mass part ratio, the moisturizing and repairing composition has the best moisturizing and repairing effects.

Preferably, the horse chestnut extract preparation method comprises the following steps:

Breaking wall of horse chestnut seeds by Gao Suzhu grinding method, and extracting with ultrasonic-assisted ethyl acetate to obtain horse chestnut extract.

s1, pre-treatment, namely drying the horse chestnut seeds, breaking the walls by Gao Suzhu grinding, and sieving for later use;

S2, coarsely extracting bioactive components, taking S1 to obtain dry powder, and soaking the dry powder in 50% -100% ethanol for 20-60 minutes, wherein the ratio of the horse chestnut seed powder to the ethanol is 1:20-1:30;

S3, extracting bioactive components, and heating the S2 solution to 80-100 ℃;

s4, extracting bioactive components with the assistance of ultrasonic waves, and carrying out ultrasonic treatment on the mixed solution of S3 for 40 minutes with ultrasonic power of 400-800W;

s5, extracting bioactive components, namely adding the mixed solution obtained in the step S4 into ethyl acetate, extracting for 1-4 hours, and taking an ethyl acetate layer;

S6, purifying the bioactive components, and carrying out suction filtration on the solution obtained in the step S5 until a clear solution is obtained;

And S7, drying the bioactive components, and drying the solution obtained in the S6 to obtain the horse chestnut extract.

Preferably, the preparation method of the celery extract comprises the following steps:

S1, pre-treatment, namely drying celery, breaking walls by Gao Suzhu grinding, and sieving for later use;

S2, removing pigments, soaking the dry powder obtained in the S1 in petroleum ether for 10-30 minutes, and then drying, wherein the ratio of the dry powder to the petroleum ether is 1:10-1:20;

s3, coarsely extracting the bioactive components, taking S2 to obtain dry powder, and soaking the dry powder in 50% -100% methanol for 20-60 minutes, wherein the ratio of the dry powder to the methanol is 1:20-1:30;

S4, extracting bioactive components, and heating the S3 solution to 80-100 ℃;

s5, extracting bioactive components, adding butanol into the mixed solution obtained in the S4, extracting for 1-4 hours, and taking a butanol layer

And S7, drying the bioactive components, and drying the solution obtained in the step S6 to obtain the celery extract.

Preferably, the preparation method of the hedyotis diffusa extract comprises the following steps:

S1, pre-treatment, namely drying the hedyotis diffusa, breaking the wall by Gao Suzhu grinding, and sieving for later use;

S3, coarsely extracting the bioactive components, taking S2 to obtain dry powder, and soaking the dry powder in 50% -100% ethanol for 20-60 minutes, wherein the ratio of the dry powder to the ethanol is 1:20-1:30;

S4, extracting bioactive components, and heating the S3 solution to 80-100 ℃;

s5, extracting bioactive components with the assistance of ultrasonic waves, and carrying out ultrasonic treatment on the mixed solution of S4 for 20-40 minutes with ultrasonic power of 400-800W;

S6, extracting the bioactive components, adding ethyl acetate into the mixed solution obtained in the step S5, extracting for 1-4 hours, and taking an ethyl acetate layer;

S7, purifying the bioactive components, and carrying out suction filtration on the solution obtained in the step S6 until a clear solution is obtained;

and S8, drying the bioactive components, and drying the solution obtained in the step S7 to obtain the hedyotis diffusa extract.

The invention also provides application of the moisturizing repair composition in cosmetics.

The invention also provides a cosmetic containing the moisturizing and repairing composition. The cosmetics may be in the form of cream, lotion, essence, facial mask, etc., but are not limited thereto.

Preferably, the moisture-preserving repair composition comprises 1-8% by weight of the cosmetic;

When the moisturizing and repairing composition is applied to cosmetics in the weight percentage, the moisturizing and repairing effects of the cosmetics can be effectively improved.

The invention also provides a moisturizing repair emulsion which comprises the following components in percentage by weight:

the moisturizing repair composition comprises 1% -8% of moisturizing repair composition, 15% -25% of humectant, 0.5% -2% of surfactant, 1% -5% of antioxidant, 2% -6% of thickener and the balance of water. The moisturizing repair emulsion of the formula disclosed by the invention is mild and non-irritating to skin, is safe to use, has an excellent moisturizing repair effect, and is suitable for various skin types.

Preferably, the humectant is glycerin.

Preferably, the surfactant is betaine.

Preferably, the antioxidant is at least one of honey and citric acid. When the antioxidant consists of honey and citric acid, the honey and the citric acid can be mixed in any proportion.

Preferably, the thickener is sodium carboxymethyl cellulose.

Preferably, the moisturizing repair emulsion comprises the following components in percentage by weight:

the moisturizing repair composition comprises 5% of a moisturizing repair composition, 15.15% of a humectant, 0.15% of a surfactant, 0.45% of an antioxidant, 0.61% of a thickener and the balance of deionized water. The moisturizing repair emulsion prepared by the formula is mild and has no stimulation to skin, is safe to use, has excellent moisturizing repair effect, and is suitable for various skin types.

Preferably, the preparation method of the moisturizing repair emulsion comprises the following steps:

(1) Adding the humectant, the surfactant, the antioxidant and the thickener into a beaker, adding deionized water, stirring at 60-75 ℃ until the mixture is uniformly mixed, and sterilizing at high pressure to obtain a mixed solution 1

(2) When the temperature of the mixed solution 1 is reduced to 45 ℃, adding a surfactant and an antioxidant, and uniformly stirring; and (3) when the temperature is reduced to the room temperature, adding the moisturizing repair composition, and stirring until the mixture is uniform to obtain the moisturizing repair emulsion.

The invention has the beneficial effects that:

compared with the prior art, the invention has the beneficial effects that; the invention creatively applies the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract in cosmetics to prepare the moisturizing and repairing composition. The synergistic effect of the saponin compounds contained in the horse chestnut extract and the apigenin contained in the celery can greatly enhance the permeability of cell membranes, so that the effects of deep moisturizing, whitening, repairing and the like are achieved by bringing phenols, flavonoid substances and moisture in the witch hazel extract, the hedyotis diffusa extract and the celery extract from the epidermis layer to deeper cortex. Secondly, due to the synergistic effect of the saponin substances and the apigenin, the cell permeability is increased, so that intracellular substances can more easily enter and exit, on the basis, the unique hedyotis cinerea in the hedyotis cinerea can enter and exit the cells more rapidly to promote the metabolism of the cells, and meanwhile, rich amino acids such as aspartic acid, serine, glutamic acid, valine and the like contained in the hedyotis cinerea can timely supplement nutrients for the cells, so that the cells grow healthily. On the basis, active substances such as isorhamnetin, rutin, quercetin and the like contained in the witch hazel, vitamin E, vitamin C, calcium, potassium, zinc, magnesium and the like can effectively slow down cell aging and enhance cell viability.

Experiments show that the moisturizing repair composition can repair skin injury caused by various reasons, has the effects of resisting inflammation, relieving and moisturizing, and is suitable for various skin types. The invention discovers that the horse chestnut extract, the witch hazel extract, the common hedyotis herb extract and the celery extract with specific proportions have better effects than single use or combined use of any three of the above extracts under the premise of the same use amount, and generate synergistic effect, so that the moisturizing and repairing composition has better moisturizing and repairing effects. The invention also provides a formula for making the moisturizing and repairing composition achieve better effects on moisturizing, antioxidation and skin repairing, and also provides a preferable formula for applying the moisturizing and repairing composition to emulsion.

Drawings

FIG. 1 is a schematic illustration of a device; examples 5 to 11 and blank cytotoxicity test.

Fig. 2 is: examples 5to 11 and comparative examples 5to 12 were tested for their antioxidant capacity.

FIG. 3 is a diagram; examples 5 to 11 and comparative examples 5 to 12 were tested for moisturizing effect over 1 to 4 hours.

Fig. 4 is: anti-inflammatory ability test of examples 5 to 11 and comparative examples 5 to 12.

Detailed Description

For a better illustration of the objects, technical solutions and advantages of the present invention, the present invention is further illustrated by the following examples. It should be understood that the embodiments of the present invention are only for illustrating the technical effects of the present invention, but not for limiting the scope of the present invention, and the methods used in the embodiments are conventional methods unless specifically described otherwise.

The sources of part of raw materials in the invention are as follows:

Horse chestnut: purchased from Shanghai Shiadad Standard technical service Co., ltd

Hamamelis mollis extract, available from Shanyang Union Biotechnology Co., ltd

Celery leaf: purchased from farmer markets

Root of Shedan grass: purchased from Shanghai Shiadad Standard technical service Co., ltd

Example 1

The embodiment provides a moisturizing repair composition which comprises the following components in parts by weight:

10 parts of horse chestnut extract, 12 parts of witch hazel extract, 5 parts of hedyotis diffusa extract and 25 parts of celery leaf extract.

Example 2

10 parts of horse chestnut extract, 8 parts of witch hazel extract, 3 parts of hedyotis diffusa extract and 27 parts of celery leaf extract.

Example 3

15 parts of horse chestnut extract, 5 parts of witch hazel extract, 3 parts of hedyotis diffusa extract and 25 parts of celery leaf extract.

Example 4

12 parts of horse chestnut extract, 15 parts of witch hazel extract, 6 parts of hedyotis diffusa extract and 30 parts of celery leaf extract.

Example 5

The embodiment provides a moisturizing repair emulsion which comprises the following components in percentage by weight:

The moisturizing repair composition of example 1 was 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium hydroxymethylcellulose 0.61% and the balance deionized water.

Example 6

5% of the moisturizing repair composition of example 2, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Example 7

The moisturizing repair composition of example 3 was 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium carboxymethylcellulose 0.61% and the balance deionized water.

Example 7

Example 8

the moisturizing repair composition of example 4 was 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium carboxymethylcellulose 0.61% and the balance deionized water.

Example 9

1% of the moisturizing repair composition of example 2, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Example 10

6% of the moisturizing repair composition of example 2, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Example 11

8% of the moisturizing repair composition of example 2, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Example 12

This example provides a method for preparing the moisturizing repair emulsion of examples 5 to 11, comprising the steps of:

S1, adding the humectant, the surfactant, the antioxidant and the thickener into a beaker, adding deionized water, stirring at 60-75 ℃ until the mixture is uniform, and sterilizing at high pressure to obtain a mixed solution 1

S2, when the temperature of the mixed solution 1 is reduced to 45 ℃, adding a surfactant and an antioxidant, stirring until the mixture is uniform, cooling to room temperature, adding the moisturizing repair composition, and continuing stirring until the mixture is uniform.

Example 13

This example provides a method for preparing horse chestnut extract, celery extract and hedyotis diffusa extract used in the above examples 1 to 4, comprising the following steps:

S1 pretreatment: drying the sample, breaking the wall by Gao Suzhu grinding, and sieving for later use;

S2, decoloring: soaking the dry powder obtained in the step S1 in petroleum ether for 10-30 minutes, and then drying, wherein the ratio of the dry powder to the petroleum ether is 1:10-1:20;

S3, crude extraction of bioactive components: soaking the dry powder obtained in the step S2 in methanol or ethanol for 45 minutes, wherein the ratio of the dry powder of the step S2 to the methanol or ethanol is 1:20-1:30;

s4, extracting bioactive components: heating the S2 solution to 85-90 ℃;

s5, extracting bioactive components by ultrasonic assistance: ultrasonic treatment is carried out on the mixed solution of S3 for 30-45 minutes, and the ultrasonic power is 400-800W;

S6, extracting bioactive components: adding ethyl acetate or butanol into the mixed solution obtained in the step S5, extracting for 1-4 hours, and taking an ethyl acetate or butanol layer;

and S8, drying the bioactive components, and drying the solution obtained in the step S7 to obtain the plant extract.

TABLE 1 example 13 preparation of starting materials and their condition parameters

Comparative example 1

A moisturizing repair composition, which consists of the following components in parts by weight:

10 parts of horse chestnut extract, 12 parts of witch hazel extract and 5 parts of hedyotis diffusa extract.

Comparative example 2

10 parts of horse chestnut extract, 8 parts of witch hazel extract and 27 parts of celery extract.

Comparative example 3

15 parts of horse chestnut extract, 3 parts of hedyotis diffusa extract and 25 parts of celery extract.

Comparative example 4

12 parts of witch hazel extract, 6 parts of hedyotis diffusa extract and 30 parts of celery extract.

Comparative example 5

The moisturizing repair emulsion consists of the following components in percentage by weight:

Horse chestnut extract 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium hydroxymethyl cellulose 0.61% and deionized water in balance.

Comparative example 6

5% of celery extract, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Comparative example 7

5% of a hedyotis diffusa extract, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Comparative example 8

5% of witch hazel extract, 15.15% of glycerol, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Comparative example 9

Comparative example 1 comprises 5% of moisturizing repair composition, 15.15% of glycerin, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Comparative example 10

Comparative example 2 the moisturizing repair composition 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium hydroxymethylcellulose 0.61% and the balance deionized water.

Comparative example 11

Comparative example 3 comprises 5% of moisturizing repair composition, 15.15% of glycerin, 0.15% of betaine, 0.15% of honey, 0.3% of citric acid, 0.61% of sodium hydroxymethyl cellulose and the balance of deionized water.

Comparative example 12

Comparative example 4 has a moisturizing repair composition of 5%, glycerin 15.15%, betaine 0.15%, honey 0.15%, citric acid 0.3%, sodium carboxymethylcellulose 0.61%, and the balance deionized water.

The method for preparing the moisturizing repair compositions of comparative examples 1 to 4 is described in example 13.

The preparation method of the moisturizing repair emulsion of comparative examples 5 to 12 is described in example 9.

Safety test

The moisturizing repair compositions of examples 1 to 4, the moisturizing repair emulsions of examples 5 to 11, the moisturizing repair compositions of comparative examples 1 to 4, and the moisturizing repair emulsions of comparative examples 5 to 12 were evaluated for cosmetic irritation by using 2015 "cosmetic safety Specification" as a reference standard, and 100 persons with ages 16 to 65 were tested by skin patch test.

The testing method comprises the following steps: putting the test object into a spot tester with the dosage of 0.020-0.025 g, covering the spot tester with the test object on the back or the curved side of the forearm of the subject by using a non-stimulated cloth-based adhesive tape, lightly pressing the test object with the palm to uniformly adhere the test object on the skin surface for 24 hours, removing the spot tester for 30 minutes, and observing skin reaction after the indentation disappears. If the result is negative, the observation is carried out again 24 hours and 48 hours after the patch test, respectively.

Evaluation criteria: level 0: a negative reaction; stage 1: suspicious reactions, only weak erythema; 2 stages: weak positive response, erythema, infiltration, edema, and possibly papules; 3 stages: strong positive reaction, erythema, infiltration, edema, papule, and reaction beyond the test area; 4 stages: very strong positive response, marked erythema, severe infiltration, edema, blepharospermia, and response beyond the test area.

Test results: all subjects were negative for skin response.

The test results show that the moisturizing repair composition and the moisturizing repair emulsion provided by the invention are mild and have no stimulation to skin and are safe to use.

Cytotoxicity test

MTT experiments were performed to verify cytotoxicity of examples 1-4.

Human immortalized epidermal cells (HaCaT) were seeded into 96-well cell culture plates at a density of 3x10 ³. After 24 hours, the medium was replaced with a medium containing 8% of the compositions of examples 1 to 4, the blank was added with an equal amount of water, then further incubated for 24 hours, and the cells were treated with MTT at a concentration of 5mg/ml and incubated for 1 hour at 37℃in a light-shielded state. Thereafter, the medium was removed and DMSO was added to dissolve the sample, and absorbance of the dissolved sample at 570nm was measured.

TABLE 2 cytotoxicity assay

Experimental group	Cell viability%
		Example 1	99.725
Example 2	99.897
		Example 3	99.638
Example 4	99.476
		Blank space	100

As can be seen from the experimental results in Table 2, the cell viability remained at about 99.89% when treated with the compositions of examples 1-4 added at a maximum dose of 8%. From this result, it was confirmed that even when treated with 8% of the moisturizing repair composition, the cells can be actively divided, and cytotoxicity was not observed.

Third, antioxidant test

The antioxidant capacity of examples 5 to 11 was verified using DPPH radical.

DPPH solution with concentration of 0.1mmol/L cosmetic emulsions of examples 5-11 and comparative examples 5-12 were prepared as 10g/L sample solution, positive control Vc solution and blank aqueous solution. 0.1ml of the sample solution was aspirated and reacted with 4.9mlDPPH ml of the solution in a dark room for 20 minutes, and then absorbance was measured at 517nm wavelength, and the clearance was calculated.

TABLE 3DPPH radical scavenging Rate

Experimental group	DVC％
		Example 5	94.13
Example 6	96.42
		Example 7	92.77
Example 8	90.49
		Example 9	95.17
Example 10	96.39
		Example 11	96.31
Comparative example 5	72.11
		Comparative example 6	70.52
Comparative example 7	67.71
		Comparative example 8	71.66
Comparative example 9	81.19
		Comparative example 10	82.44
Comparative example 11	80.28
		Comparative example 12	84.22
Vc	100

From the results of examples 5 to 11 in Table 3, it is shown that the moisturizing repair emulsion provided in the examples of the present invention has good antioxidation, especially the effects of examples 6 and 9 to 11 are good, which means that the moisturizing repair composition has the best antioxidation effect when the mass fraction ratio of horse chestnut extract, witch hazel extract, sheganella extract and celery leaf extract is 1:0.8:0.3:2.7. In examples 6 and 9 to 11, the best effect of example 6 shows that the antioxidant effect of the moisturizing repair composition emulsion of different components has a certain influence, and the moisturizing repair composition added in an amount of 5% has the best antioxidant effect.

The results of comparative examples 5 to 11 and comparative examples 5 to 8 show that the combination of the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery leaf extract has a synergistic effect, and the antioxidation effect of the moisturizing repair emulsion can be obviously improved.

As can be seen from comparing the results of examples 5 to 11 with those of comparative examples 9 to 12, the moisturizing repair composition provided by the present invention can effectively improve the antioxidation effect of the emulsion.

Fourth, moisture-preserving effect test

Cosmetic moisturization was evaluated using a skin moisture meter CorneometerCM for 825, and 100 persons aged 16 to 65 were tested for random distribution of the moisturizing restoration emulsions of examples 5 to 11 and the moisturizing restoration emulsions of comparative examples 5 to 12.

The testing method comprises the following steps:

The moisturizing repair emulsions of examples 5 to 11 and comparative examples 5 to 12 were applied to the same parts of the subjects, and the skin moisture content of the applied areas of each subject was measured using a skin moisture content meter, once every 1 hour, and the values in the applied areas of each subject were read every test, and the average values of each group were calculated for 4 times.

Test results: as shown in table 4 below.

Table 4 moisture retention effect test

Experimental group	1 Hour	For 2 hours	3 Hours	4 Hours
					Example 5	84％	81％	69％	61％
Example 6	86％	82％	71％	65％
					Example 7	85％	81％	73％	66％
Example 8	82％	77％	68％	60％
					Example 9	85％	81％	70％	64％
Example 10	84％	82％	72％	63％
					Example 11	86％	82％	71％	65％
Comparative example 5	77％	66％	31％	21％
					Comparative example 6	75％	62％	27％	17％
Comparative example 7	72％	65％	34％	26％
					Comparative example 8	74％	70％	42％	31％
Comparative example 9	78％	75％	55％	42％
					Comparative example 10	76％	74％	57％	41％
Comparative example 11	79％	72％	52％	44％
					Comparative example 12	77％	72％	54％	44％

Analysis of results: from the results of examples 5 to 11, the moisturizing repair emulsion provided by the embodiment of the invention has better moisturizing effect, especially the effects of examples 6 and 9 to 11 are better, which indicates that the moisturizing effect of the moisturizing repair composition rollback emulsion with different components is influenced to a certain extent. The moisturizing repair composition has the best moisturizing effect when the mass fraction ratio of the horse chestnut extract to the witch hazel extract to the common vetch extract to the celery leaf extract is 1:0.8:0.3:2.7. In examples 6 and 9 to 11, the best effect of example 6 shows that the moisturizing effect of the moisturizing repair composition emulsions of different components is affected to some extent, and the moisturizing repair emulsion added in an amount of 5% of the moisturizing repair composition has the best moisturizing effect.

The results of comparative examples 5 to 11 and comparative examples 5 to 8 show that the combination of horse chestnut extract, witch hazel extract, ophiopogon root extract and celery leaf extract has synergistic effect, and the moisturizing effect of the moisturizing repair emulsion can be obviously improved.

As can be seen from comparing the results of examples 5 to 11 with those of comparative examples 9 to 12, the moisturizing repair composition provided by the present invention can effectively improve the moisturizing effect of the emulsion.

Fifthly, inflammation inhibition test

Anti-inflammatory evaluation was performed on the moisturizing repair emulsions of examples 5 to 11 and the moisturizing repair emulsions of comparative examples 5 to 12 using a model of swelling of ears of mice due to xylene.

Laboratory animals, kunming mice, SPF grade, guangzhou Sier Baimo Biotechnology Inc., license number SCXK (Guangdong) 2020-0055. The weight of the body is 18-20 g, the male and female parts are respectively half, 140 body weight is divided into 17 groups, and 10 body weight is divided into 10 body weight groups. The 17 groups of animals were:

blank group: the right ear of the mouse is uniformly smeared with 0.05ml of dimethylbenzene, and after 1.5 hours, cervical vertebra dislocation is killed, and the left ear and the right ear are punched and weighed.

Positive group: the right ear of the mouse is uniformly smeared with 0.05ml of dimethylbenzene, after 30 minutes, the right ear is smeared with 0.1g of dermatitis, after 1 hour, cervical vertebra dislocation is killed, and the left ear and the right ear are punched and weighed.

Experimental group: the right ear of the mouse is uniformly smeared with 0.05ml of dimethylbenzene, 30 minutes later, the right ear is smeared with 0.1g of moisturizing repair emulsion of examples 5-11 and comparative examples 5-12, after 1 hour, cervical dislocation is killed, and the left ear and the right ear are punched and weighed.

The test method comprises the following steps: the xylene is coated on the ear of a mouse, so that local tissue inflammation can be caused, inflammatory substances are released, and acute exudative edema of the ear is caused. Mice were randomly divided into 14 groups, namely, a blank group, a positive group and an experimental group, ten each. The right ears of the mice of each group are uniformly coated with 0.05ml of dimethylbenzene, after 30 minutes, the right ears of the mice are coated with corresponding medicaments respectively except for a blank group, each group is 0.1g of the medicament, the medicament is used for 60 minutes, cervical dislocation is killed, ears of the same parts of ears of the mice are respectively punched by a 7mm puncher, the ears are weighed, the difference of the weights of the ears is taken as the swelling degree, and the average swelling degree of each group is calculated, so that the results are shown in Table 5.

TABLE 5 test for inhibition of inflammation

Analysis of results: from the results of examples 5 to 11, the moisturizing repair emulsion provided by the embodiment of the invention has better anti-inflammatory effect, and particularly, the effects of examples 6 and 9 to 11 are better, which indicates that the moisturizing repair composition has the best anti-inflammatory effect when the mass fraction ratio of the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery leaf extract is 1:0.8:0.3:2.7. In examples 6 and 9 to 11, the best effect of example 6 shows that the anti-inflammatory effect of the moisturizing repair composition emulsion of different components has a certain influence, and the moisturizing repair composition added in an amount of 5% has the best anti-inflammatory effect.

The results of comparative examples 5 to 11 and comparative examples 5 to 8 show that the combination of horse chestnut extract, witch hazel extract, ophiopogon root extract and celery leaf extract has synergistic effect, and can obviously improve the anti-inflammatory effect of the moisturizing repair emulsion.

As can be seen from comparing the results of examples 5 to 11 with those of comparative examples 9 to 12, the moisturizing and repairing composition provided by the present invention can effectively improve the anti-inflammatory effect of the emulsion.

The embodiments described above are some, but not all embodiments of the application. The detailed description of the embodiments of the application is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

Claims

1. The moisturizing repair composition is characterized by comprising the following components in parts by mass:

10-15 parts of horse chestnut extract;

5-15 parts of witch hazel extract;

3-6 parts of a common hedyotis herb extract;

25-30 parts of celery leaf extract.

2. The moisturizing repair composition according to claim 1, wherein the parts ratio of the horse chestnut extract, the witch hazel extract, the hedyotis diffusa extract and the celery extract is 1:0.5-1.2:0.1-0.5:2.5-3.

3. The moisturizing repair composition according to claim 1, wherein the composition comprises the following components in parts by mass: 10 parts of horse chestnut extract, 8 parts of witch hazel extract, 3 parts of hedyotis diffusa extract and 27 parts of celery extract.

4. The cosmetic composition for skin protection according to claim 1, comprising horse chestnut extract, witch hazel extract, ophiopogon root extract and celery extract, wherein the at least one extract is extracted using any one of or a mixture of two or more extraction solvents selected from the group consisting of anhydrous or aqueous lower alcohols having 1 to 4 carbon atoms, ethyl acetate and petroleum ether.

5. A moisturizing repair skin care product characterized by comprising the moisturizing repair composition according to any one of claims 1 to 4.

6. The skin care product according to claim 5, wherein the moisturizing and repairing composition is added in an amount of 1% to 8%.

7. The moisturizing repair composition of claim 1,

The horse chestnut extract is obtained by the following method:

Crushing horse chestnut seeds, and obtaining horse chestnut extract by adopting an ultrasonic-assisted ethyl acetate extraction method;

The hedyotis diffusa extract is obtained by the following method:

pulverizing herba Hedyotidis Diffusae, and extracting with ethyl acetate to obtain herba Hedyotidis Diffusae extract.

The celery extract is obtained by the following method:

pulverizing herba Apii Graveolentis, and performing ultrasonic assisted alcohol extraction to obtain herba Apii Graveolentis extract.

8. The moisturizing repair composition of claim 7, wherein the preparation method of the horse chestnut extract specifically comprises the following steps:

s1, soaking smashed horse chestnut seeds in ethanol for 30-45 minutes, wherein the feed-liquid ratio is 1:20-1:30;

S2, heating the S1 product to 80-90 ℃, and performing ultrasonic treatment for 20-40 minutes with ultrasonic power of 400-800W;

s3, adding 0.5-1 part by mass of ethyl acetate into the S2 product for extraction for 1-4 hours;

S4, filtering the S3 product until the product is clear;

s5, drying the S4 product to prepare the horse chestnut extract.

9. The moisturizing repair composition of claim 7, wherein the preparation method of the hedyotis diffusa extract specifically comprises the following steps:

s1, soaking smashed hedyotis diffusa in petroleum ether for 10-30 minutes; the feed-liquid ratio is 1:10-1:20, and then drying is carried out;

s2, soaking the S1 product in ethanol for 30-45 minutes, wherein the feed-liquid ratio is 1:20-1:30;

s3, heating the S2 product to 80-100 ℃ and keeping the temperature for 20-40 minutes;

S4, filtering the S3 product until the product is clear, and adding 0.5-1 part of ethyl acetate for extraction for 1-4 hours;

s5, filtering the S4 product until the product is clear;

s6, drying the S5 product to prepare the herba hedyotis diffusae extract.

10. The moisturizing repair composition according to claim 7, wherein the preparation method of the celery extract specifically comprises the following steps:

S1, soaking the smashed celery leaves in petroleum ether for 10-30 minutes. The feed-liquid ratio is 1:10-1:20, and then drying is carried out;

s2, soaking the S1 product in methanol for 30-45 minutes, wherein the feed-liquid ratio is 1:20-1:30;

s3, heating the S2 product to 80-100 ℃, and performing ultrasonic treatment for 20-40 minutes with ultrasonic power of 400-800W;

s4, adding 0.5-1 mass part of butanol into the S3 product, and extracting for 1-4 hours;

s5, filtering the S4 product until the product is clear;

s6, drying the S5 product to prepare the celery extract.