CN117918523A - Exosome-containing special food for dispelling effects of alcohol and protecting liver and preparation method thereof - Google Patents
Exosome-containing special food for dispelling effects of alcohol and protecting liver and preparation method thereof Download PDFInfo
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses an exosome-containing special dietary food for dispelling the effects of alcohol and protecting liver and a preparation method thereof. The extraction process of the plant exosome provided by the invention is different from the traditional preparation method of the plant extract, is simple to operate, short in time consumption, free of organic reagent and high-temperature treatment, and effectively protects active substances in plants. The key raw materials of the special meal food for dispelling the effects of alcohol and protecting the liver are semen hoveniae exosome freeze-dried powder and radix puerariae exosome freeze-dried powder, so that the effects of dispelling the effects of alcohol and protecting the liver can be better exerted, and the special meal food has the characteristics of strong efficacy, lower cost and higher safety.
Description
Technical Field
The invention belongs to the technical field of functional foods, and particularly relates to an exosome-containing alcohol-dispelling and liver-protecting special dietary food and a preparation method thereof.
Background
The most common hazard caused by excessive drinking is acute alcoholism, which can cause nervous problems such as vomiting, hypomnesis, inattention, and unstable emotion. Long-term excessive drinking is likely to cause mental disorder, cognition error, fatty liver, alcoholic hepatitis, liver cirrhosis and other diseases, has direct damage to human viscera, and seriously endangers life, and research from the medical and pathological aspects proves that drinking can also cause cancers and cardiovascular and cerebrovascular diseases. The world health organization (RHO) states that alcoholism is one of the public nuisances worldwide today. Statistics of drinking data in 195 countries worldwide show that mortality and disability adjustment years due to long-term excessive drinking are ranked seventh in health risk behaviors.
At present, the existing anti-alcoholic products in the markets at home and abroad are roughly divided into four types of chemical medicines, chinese herbal medicine preparations, health-care products and drinks. The chemical medicine has the anti-alcohol effect through the symptomatic treatment strategy, belongs to a single excitation type, reduces the accumulation of acetaldehyde in the body through the effect of the plasma hormone, reduces the inhibition effect of the chemical medicine on the central nervous system of the human body, and plays the role of anti-alcohol. Or promote the excretion of alcohol from the body by diuretics. The health care product can integrate novel components supported by the theory and theoretical research of traditional Chinese medicines, but has the problems of slow alcohol effect and single efficacy, and cannot meet the practical demands of people. The traditional Chinese medicine preparation takes the traditional Chinese medicine extract as a functional component, not only can play the effect of dispelling the effects of alcohol, but also can relieve viscera damage caused by long-term drinking, but the purity of the effective component of the traditional Chinese medicine extract is lower, and most of the effective component is destroyed and decomposed by enzymes, acid and other environments through the oral cavity and the stomach, so that the content of the effective component is reduced, the efficacy of the effective component is influenced, and the efficacy of a product is reduced by using the raw materials under the condition of the same dose of related raw materials. The three existing anti-alcoholic beverages have poor anti-alcoholic effect, poor flavor and preservative, and can cause 2 damages to the liver after long-term massive drinking. Therefore, the special meal product which can rapidly relieve the alcohol and protect the liver from being damaged, is safe and effective and can carry out accurate nutrition supplement is an important research direction.
Disclosure of Invention
The invention aims to provide a special dietary food for dispelling effects of alcohol and protecting liver and a preparation method thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention comprises the following steps:
An exosome-containing special dietary food for relieving alcoholic intoxication and protecting liver comprises semen Hoveniae exosome lyophilized powder and radix Puerariae exosome lyophilized powder.
The preparation method of the hovenia dulcis thunb exosome freeze-dried powder and the kudzuvine root exosome freeze-dried powder comprises the following steps:
1) Cleaning plants with purified water to remove surface impurities for standby;
2) Crushing the plants by adopting a physical means, then adding the crushed plants and a buffer solution into a wall breaking machine according to the weight ratio of 1:1, and squeezing juice for 15min to obtain plant homogenate;
3) Transferring the plant homogenate obtained in the step 2) into a centrifugal container, centrifuging at 4 ℃ and 4500r/min for 20min, and removing residues to obtain upper liquid;
4) Centrifuging the supernatant obtained in the step 3) at 4 ℃ and 10000r/min for 60min, collecting supernatant to obtain plant exosome extract, and lyophilizing;
Wherein the plant is one or more of radix Puerariae and semen Hoveniae;
Wherein the buffer is physiological saline.
A preparation method of a special dietary food containing exosomes for relieving alcoholic intoxication and protecting liver comprises the following steps:
(1) Preparing raw materials and auxiliary materials: 20-22g of hovenia dulcis thunb exosome freeze-dried powder, 18-20g of radix puerariae exosome freeze-dried powder, 3-5g of rice bran fatty alkanol, 5-8g of L cysteine, 10.04-0.06g of vitamin B, 20.04-0.06g of vitamin B, 16-20g of sorbitol and 5-8g of microcrystalline cellulose;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), and adding the mixture into a mixer in 3 times of equal amount to obtain a premix;
(3) Preparing a soft material: dissolving 0.5-0.8g povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading until the thickness is not more than 0.5cm, putting into a baking oven for baking, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.5-0.8g of magnesium stearate into the dry powder, uniformly mixing, adding into a mixer in 3 times of equal amount, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
In the step (2) and the step (5), after each time of feeding into a mixer, stirring is needed for 3-5min, and then feeding is continued.
In the step (4), the conditions for oven drying are as follows: and (3) drying at 40 ℃ for 20min, and measuring the moisture content to be less than 5%. The beneficial effects of the invention are as follows:
1. the formula of the plant exosome with the functions of dispelling the effects of alcohol and protecting the liver, rice bran fatty alkanol, amino acid and vitamin raw material special meal tablet is guided by the basic theory and scientific alcohol mechanism of traditional Chinese medicine, and meanwhile, the modern accurate nutrition research is combined, so that the efficacy of the product is ensured.
2. The kudzuvine root exosomes and the hovenia dulcis thunb exosomes which are core efficacy raw materials used in the invention can resist the damage of gastrointestinal enzymes, increase the tolerance of acidic environment, reduce the loss of active ingredients, and enable the kudzuvine root exosomes and the hovenia dulcis thunb exosomes to better play the efficacy of the active ingredients after eating.
3. The plant exosome special meal tablet with the functions of dispelling the effects of alcohol and protecting the liver can effectively help people to expel and prolong the drunk time and shorten the sobering time, has no side effect, can avoid various complications caused by taking western medicines, is beneficial to accurate nutrition supplement of human bodies, and is suitable for specific people to take.
4. The plant exosome special meal tablet with the functions of dispelling the effects of alcohol and protecting the liver provided by the invention has the advantages that the raw materials in the formula are food raw materials or the national permission for special meal raw materials, and the dosage is strictly limited in a certain range, so that the special meal tablet is safe and effective, has no toxic or side effect after being taken for a long time, and can be eaten for a long time.
Detailed Description
The present invention will be described in further detail with reference to examples.
The materials or chemicals used in the examples of the present invention, unless otherwise specified, were obtained by conventional commercial means.
Partial raw material sources:
hovenia dulcis thunb and kudzuvine root: all are fresh raw materials which are harvested within half a year;
semen Hoveniae extract: shaanxi Dongfu Biotechnology Co., ltd;
radix Puerariae extract: shaanxi Dongfu Biotechnology Co., ltd;
rice bran fatty alkanols: hong Ying a large health group, raw material producing area: japan;
L cysteine; guangzhou Hua Xi bioengineering Co., ltd;
Vitamin B1: shuowei nutrition technology (Guangzhou) limited;
vitamin B2: shuowei nutrition technology (Guangzhou) limited;
Sorbitol: sorbitol TSG65; lot number: 452301015; the manufacturer: shandong Tianli pharmaceutical Co., ltd;
microcrystalline cellulose: hebei hundred Biotech Co., ltd;
Magnesium stearate: hebei hundred Biotech Co.
Example 1:
1. extraction and preparation of plant exosomes:
(1) Cleaning plants (semen Hoveniae and radix Puerariae in the invention) with purified water to remove surface impurities for standby.
(2) Crushing plants by adopting a physical means, putting the raw materials and a buffer solution into a juicer (in the invention, hovenia dulcis thunb, kudzuvine root) according to the weight ratio of 1:1, juicing for 15min to obtain crushed plant homogenate, and transferring the crushed plant homogenate into a centrifugal container.
(3) Centrifuging the plant homogenate obtained in step (2) at 4deg.C and 4500r/min for 20min, and removing residues to obtain upper liquid.
(4) Centrifuging the upper liquid obtained in the step (3) at 4 ℃ and 10000r/min for 60min, and reserving the upper liquid to obtain the plant exosome extract. And then carrying out optimal freeze-drying process conditions, and freeze-drying the product.
2. The preparation method of the special meal slice comprises the following steps:
(1) Preparing raw materials and auxiliary materials: 21g of raisin tree seed exosome freeze-dried powder, 19g of kudzuvine root exosome freeze-dried powder, 4g of rice bran fatty alkanol, 6g of L cysteine, 10.05g of vitamin B, 20.045g of vitamin B, 18g of sorbitol and 6g of microcrystalline cellulose;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), adding the mixture into a mixer in 3 times of equal amount, and stirring for 3-5min after each feeding to obtain a premix;
(3) Preparing a soft material: dissolving 0.7g povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading, placing into an oven for drying at 40 ℃ for 20min, measuring moisture content to be less than 5%, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.6g of magnesium stearate into the dry powder, uniformly mixing, adding into a mixer in 3 times of equal amount, stirring for 3-5min after each feeding, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
Note that: all the steps are completed in the clean area.
Example 2:
1. extraction and preparation of plant exosomes:
(1) Cleaning plants (semen Hoveniae and radix Puerariae in the invention) with purified water to remove surface impurities for standby.
(2) Crushing plants by adopting a physical means, putting the raw materials and a buffer solution into a juicer (in the invention, hovenia dulcis thunb, kudzuvine root) according to the weight ratio of 1:1, juicing for 15min to obtain crushed plant homogenate, and transferring the crushed plant homogenate into a centrifugal container.
(3) Centrifuging the plant homogenate obtained in step (2) at 4deg.C and 4500r/min for 20min, and removing residues to obtain upper liquid.
(4) Centrifuging the upper liquid obtained in the step (3) at 4 ℃ and 10000r/min for 60min, and reserving the upper liquid to obtain the plant exosome extract. And then carrying out optimal freeze-drying process conditions, and freeze-drying the product.
2. The preparation method of the special meal slice comprises the following steps:
(1) Preparing raw materials and auxiliary materials: 22g of raisin tree seed exosome freeze-dried powder, 20g of kudzuvine root exosome freeze-dried powder, 5g of rice bran fatty alkanol, 8g of L cysteine, 10.06g of vitamin B, 20.06g of vitamin B, 20g of sorbitol and 8g of microcrystalline cellulose;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), adding the mixture into a mixer in3 times of equal amount, and stirring for 5 minutes after each feeding to obtain a premix;
(3) Preparing a soft material: dissolving 0.8g povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading, placing into an oven for drying at 40 ℃ for 20min, measuring moisture content to be less than 5%, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.8g of magnesium stearate into the dry powder, uniformly mixing, adding the mixture into a mixer in 3 times of equal amount, stirring for 5min after each feeding, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
Note that: all the steps are completed in the clean area.
Example 3:
1. extraction and preparation of plant exosomes:
(1) Cleaning plants (semen Hoveniae and radix Puerariae in the invention) with purified water to remove surface impurities for standby.
(2) Crushing plants by adopting a physical means, putting the raw materials and a buffer solution into a juicer (in the invention, hovenia dulcis thunb, kudzuvine root) according to the weight ratio of 1:1, juicing for 15min to obtain crushed plant homogenate, and transferring the crushed plant homogenate into a centrifugal container.
(3) Centrifuging the plant homogenate obtained in step (2) at 4deg.C and 4500r/min for 20min, and removing residues to obtain upper liquid.
(4) Centrifuging the upper liquid obtained in the step (3) at 4 ℃ and 10000r/min for 60min, and reserving the upper liquid to obtain the plant exosome extract. And then carrying out optimal freeze-drying process conditions, and freeze-drying the product.
2. The preparation method of the special meal slice comprises the following steps:
(1) Preparing raw materials and auxiliary materials: semen hoveniae exosome freeze-dried powder 20g, radix puerariae exosome freeze-dried powder 18g, rice bran fatty alkanol 3g, L cysteine 5g, vitamin B10.04g, vitamin B20.04g, sorbitol 16g and microcrystalline cellulose 5g;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), adding the mixture into a mixer in3 times of equal amount, and stirring for 3 minutes after each feeding to obtain a premix;
(3) Preparing a soft material: dissolving 0.5 povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading, placing into an oven for drying at 40 ℃ for 20min, measuring moisture content to be less than 5%, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.5g of magnesium stearate into the dry powder, uniformly mixing, adding into a mixer in 3 times of equal amount, stirring for 3min after each feeding, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
Note that: all the steps are completed in the clean area.
Example 4:
The preparation method of the special meal slice comprises the following steps:
(1) Preparing raw materials and auxiliary materials: 21g of semen hoveniae extract, 19g of radix puerariae extract, 4g of rice bran fatty alkanol, 6g of L cysteine, 10.05g of vitamin B, 20.045g of vitamin B, 18g of sorbitol and 6g of microcrystalline cellulose;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), adding the mixture into a mixer in3 times of equal amount, and stirring for 4 minutes after each feeding to obtain a premix;
(3) Preparing a soft material: dissolving 0.7g povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading, placing into an oven for drying at 40 ℃ for 20min, measuring moisture content to be less than 5%, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.6g of magnesium stearate into the dry powder, uniformly mixing, adding into a mixer in 3 times of equal amount, stirring for 4min after each feeding, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
Note that: all the steps are completed in the clean area.
Experiment 1:
The formulations described in example 1, example 2, example 3 and example 4 were tested for anti-hangover effect, and SD rat experiments were specifically as follows:
1. The purpose of the experiment is as follows:
In the experiment, after the SD rat is subjected to gastric lavage by using white spirit, an SD rat drunk model is constructed, and the drunk and sobering-up time change condition of the SD rat after sample treatment is counted, so that the anti-alcohol effect of the sample is evaluated.
2. Experimental animal
The animal used in this experiment is SD rat, sex male, and weight is 180-200g.
3. Experimental reagent
Festuca arundinacea (50 °), example 1, example 2, example 3, example 4.
4. Experimental grouping:
a first group: the blank control group, five rats (respectively marked as left ear, right ear, double ear, tail cutting and normal) and recording body weight;
Second group: a negative control group, five rats (labeled left ear, right ear, binaural, tail-cut, normal, respectively) and recording body weight;
Third group: SD rats of eating example 1 were a group, a+ wine group, fifteen (labeled left ear, right ear, binaural, tail-cut, normal, respectively) rats, divided into three groups, five A1, five A2, five A3, and body weights were recorded.
Fourth group: the SD rats of eating example 2 were group B, group b+wine, fifteen (labeled left ear, right ear, double ear, tail-cut, normal, respectively) rats, divided into three groups, five of B1, five of B2, five of B3, and body weights were recorded.
Fifth group: the SD rats of eating example 3 were C group, c+ wine group, fifteen (labeled left ear, right ear, binaural, tail-cut, normal, respectively) rats, divided into three groups, C1 five, C2 five, C3 five, and body weights were recorded.
5. The experimental steps are as follows:
(1) Fasted overnight (no food in 12 h) before the experiment, and was free to drink water.
(2) Group a, weighing example 1:6g, dissolving with normal saline and fixing the volume to 100ml for later use; group B, weighing example 2:6g, dissolving with normal saline and fixing the volume to 100ml for later use; group C, weighing example 3:6g, dissolving with normal saline and fixing the volume to 100ml for later use;
(3) After the start of the experiment, group a: group A1 at 0.075ml/100g, group A2 at 0.1ml/100g, group A3 at 0.125ml/100g; group B: group B1 was irrigated with 0.075ml/100g, group B2 was irrigated with 0.1ml/100g, group B3 was irrigated with 0.125ml/100g, group C5, group C1 was irrigated with 0.075ml/100g, group C2 was irrigated with 0.1ml/100g, group C3 was irrigated with 0.125ml/100g, and the blank and model groups were irrigated with equal amounts of saline.
(4) After 30min, each group was perfused with festuca arundinacea at a dose of 0.05mL/10g, except for the blank group.
5. Experiment result statistics:
The rat is unstable in crawling, the back of the rat drags the ground, the loss time of the regular reflection is turned over (the back of the rat keeps 30 seconds downwards), and the rat is lazy to sleep as an index of drunk;
-restoring spirit to an awake (sober-up) index with free movement, flexible limbs, regular reflex recovery time; the time to intoxication and the time to sober (unit min, time to sober refers to the time from intoxication to sober-up) were recorded for each group of rats.
TABLE 1 Effect of example 3 on anti-hangover SD rats
Note that: * Significant differences (P < 0.05) compared to model group; there was a very significant difference (P < 0.01) compared to the model group.
As can be seen from table 1, the results show that the solutions of the anti-hangover and liver-protecting compositions with different concentrations can prolong the time for the SD rats to be drunk and shorten the recovery time, wherein the recovery time is obviously shortened in the medium-dose and high-dose groups compared with the model group, and the recovery time is obviously different (P < 0.05). The high dose has a very significant difference (P < 0.01) compared to the model group, extending the time of onset of intoxication.
Compared with the negative model group, the SD rats in the example 1, the example 2 and the example 4 have longer drunk occurrence time and shorter recovery time, and the effect is obviously better than that of the comparison example group. Among them, in the examples 1 and 2, there was a significant difference (P < 0.05) in the average time to intoxication extension compared to the negative model group in the middle-and high-dose groups, and in the examples 1, 2 and 3, there was a significant difference (P < 0.05) in the average time to intoxication compared to the example 4 group.
Experiment 2:
the samples prepared in example 1, example 2, example 3 and example 4 were tested for anti-hangover effect, and the human efficacy experiments were specifically as follows:
1. The purpose of the experiment is as follows:
The experiment uses white spirit to construct a human body drunk model of middle-aged and young volunteers, and after statistical sample treatment;
drunk, sobering-up time and conditions of human body perception observation (headache, dizziness, nausea, fever of the whole body, alcohol gas in the mouth, gastrointestinal discomfort, dysphoria, palpitation and chest distress).
2. Experimental model
The health care composition is suitable for 20 people in middle-aged and young, 20 people in half of men and women, ages of 20-45 years, and has good health condition after taking a whole body physical examination before a test.
3. Experimental sample and apparatus
Festuca arundinacea (50 °), example 1, example 2, example 3, example 4.
4. Test protocol and procedure
The test adopts the self comparison method of the same volunteers, firstly completes the test of a blank control group, then completes the observation and behavior index test of the same volunteers after drinking the samples, and compares the difference of the two groups of data. Every 10 people, each half of men and women. After 5 days from the completion of the blank test, the test group test was performed.
(1) Blank control group
10 Healthy middle-aged and young volunteers, and men and women, have no frequent drinking behavior, have an average age of 28 years, have a body mass (60+/-10) kg, are fasted for 6 hours before experiments, and drink water freely.
Each person takes the white wine orally, and the white wine is unlimited. Observing and recording the drunk time, sobering-up time and physical perception condition.
(2) Experimental observation group
10 Healthy middle-aged and young volunteers, and men and women, have no frequent drinking behavior, have an average age of 28 years, have a body mass (60+/-10) kg, are fasted for 6 hours before experiments, and drink water freely.
3 Tablets of anti-alcohol product are taken by each person on an empty stomach, and white spirit is taken orally after 30 minutes, and the dosage is unlimited. Observing and recording the drunk time, sobering-up time and physical perception condition.
5. Statistics of experimental results
-Sleep (drunk) index of unstable human body movement, soft legs, heavy head and sleepiness and lazy movement
Recording the time of intoxication and the time of sobering up (unit min, sobering up time means the time from intoxication to sobering up) of the human body with the indexes of free movement, flexible limbs, clear head and restoration of spirit
Body perception observation (headache, dizziness, nausea, fever of the whole body, vintage in the mouth, gastrointestinal discomfort, dysphoria, palpitations and chest distress)
TABLE 2 Effect of examples on anti-hangover in humans
| Example 1 | Example 2 | Example 3 | Example 4 | |
| Experiment number of people (human) | 10 | 10 | 10 | 10 |
| People with anti-hangover effect | 6 | 5 | 6 | 3 |
As can be seen from table 2, the results show that the anti-hangover and liver-protecting compositions with different proportions can prolong the drunk time and shorten the recovery time of the volunteers, and play a role in relieving hangover and discomfort symptoms, wherein the example 4 has the least number of people with the effects of relieving hangover and discomfort after drunk on the volunteers.
Compared with the negative model group, the volunteer group of the example 1, the example 2 and the example 3 has longer drunk time, shorter recovery time and obviously better effect than the blank control group. The results of human body test feeding tests show that the anti-alcoholic composition preparation containing the plant Chinese herbal medicine exosomes can promote the rapid absorption and decomposition of alcohol in vivo, is beneficial to the rapid anti-alcoholic of alcohol ingesters and prevents hangover.
The embodiments of the present invention are not limited thereto, and modifications, substitutions or alterations in other various forms of the present invention according to the general knowledge and conventional means of the present art are possible within the scope of the present invention as defined by the appended claims, under the premise of the above basic technical ideas of the present invention.
Claims (5)
1. An exosome-containing alcohol-dispelling liver-protecting special meal food is characterized by comprising semen hoveniae exosome freeze-dried powder and radix puerariae exosome freeze-dried powder.
2. The exosome-containing anti-alcoholic liver protection special meal food according to claim 1, wherein the preparation method of the raisin tree seed exosome freeze-dried powder and the kudzuvine root exosome freeze-dried powder is as follows:
1) Cleaning plants with purified water to remove surface impurities for standby;
2) Crushing the plants by adopting a physical means, then adding the crushed plants and a buffer solution into a wall breaking machine according to the weight ratio of 1:1, and squeezing juice for 15min to obtain plant homogenate;
3) Transferring the plant homogenate obtained in the step 2) into a centrifugal container, centrifuging at 4 ℃ and 4500r/min for 20min, and removing residues to obtain upper liquid;
4) Centrifuging the supernatant obtained in the step 3) at 4 ℃ and 10000r/min for 60min, collecting supernatant to obtain plant exosome extract, and lyophilizing;
The plant is one or more of radix Puerariae and semen Hoveniae;
The buffer is normal saline.
3. The preparation method of the exosome-containing alcohol-dispelling and liver-protecting special meal food is characterized by comprising the following steps of:
(1) Preparing raw materials and auxiliary materials: 20-22g of hovenia dulcis thunb exosome freeze-dried powder, 18-20g of radix puerariae exosome freeze-dried powder, 3-5g of rice bran fatty alkanol, 5-8g of L cysteine, 10.04-0.06g of vitamin B, 20.04-0.06g of vitamin B, 16-20g of sorbitol and 5-8g of microcrystalline cellulose;
(2) Premixing: uniformly mixing the raw materials and the auxiliary materials in the step (1), and adding the mixture into a mixer in 3 times of equal amount to obtain a premix;
(3) Preparing a soft material: dissolving 0.5-0.8g povidone in 10ml of 95% alcohol, adding the premix, and uniformly mixing to obtain a soft material;
(4) And (3) drying: filtering the soft material into a tray through a 14-mesh screen of a vibrating screen, uniformly spreading until the thickness is not more than 0.5cm, putting into a baking oven for baking, crushing, and filtering through a 20-mesh screen of the vibrating screen to obtain dry powder;
(5) Tabletting: adding 0.5-0.8g of magnesium stearate into the dry powder, uniformly mixing, adding into a mixer in 3 times of equal amount, tabletting by using a multifunctional tabletting machine after mixing, and immediately filling into a sealing bag after tabletting, thus obtaining the anti-alcohol liver-protecting special meal food containing exosomes.
4. The method of claim 3, wherein in step (2) and step (5), the materials are continuously added after stirring for 3-5min after each addition to the mixer.
5. The method of claim 3, wherein in the step (4), the oven-drying conditions are as follows: and (3) drying at 40 ℃ for 20min, and measuring the moisture content to be less than 5%.
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