CN117899145A - Traditional Chinese medicine composition and medicine for preventing and treating alcoholic liver injury - Google Patents
Traditional Chinese medicine composition and medicine for preventing and treating alcoholic liver injury Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composition and a medicine for preventing and treating alcoholic liver injury, wherein the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 20-40 parts of kudzuvine root, 10-25 parts of hovenia dulcis thunb, 3-15 parts of bupleurum, and 5-20 parts of red paeony root. According to the specific compatibility principle of 'monarch, minister, assistant and guide', the invention fully plays the characteristics of 'simple, convenient, cheap and experimental' of the traditional Chinese medicine, and the components are mutually matched and complement each other, so that the efficacy of the raw materials is maximized under the condition of simple formula, and simultaneously, the invention has no toxic or side effect. The four medicines in the prescription are combined together to play roles of dispelling the effects of alcohol, relieving restlessness, clearing heat, promoting diuresis, soothing liver and activating blood, and simultaneously have the effects of dispelling the effects of alcohol and protecting liver, and can be used for treating acute alcoholism and chronic alcoholic liver injury.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition and a medicine for preventing and treating alcoholic liver injury.
Background
Wine is one of common drinks in China, and wine culture is a great source in China. In recent years, due to the increase of social needs and life pressure, the alcohol consumption population in China is increased, and the statistics of the alcohol consumption population exceeds 5 hundred million people. People often have symptoms of drunk, such as dizziness, vomit, inattention, hypomnesis, unstable emotion and the like, when drinking excessive wine at one time. If drinking wine for a long time, viscera dysfunction can be caused, and viscera pathological changes can occur. The alcoholic liver injury is liver toxic injury caused by long-term high-volume drinking, and comprises alcoholic fatty liver, alcoholic hepatitis, alcoholic liver fibrosis and alcoholic liver cirrhosis.
At present, the treatment of alcoholic liver injury is mainly alcohol withdrawal and nutritional support, so as to reduce the severity of alcoholic liver disease, improve the existing secondary malnutrition and symptomatically treat alcoholic liver cirrhosis and complications thereof. Among them, abstinence is the most prominent and fundamental therapeutic approach. However, for alcohol-dependent patients, the alcohol-dependent patients are difficult to completely abstain from due to repeated abstinence, and the medicine can assist in some medicine treatments, so that the spirit dependence on alcohol is reduced. Nutritional support and drug therapy are also important means of treatment. The body of the patient with alcoholic liver injury is generally lack of vitamin B, vitamin C, vitamin E and the like, and can supplement proper amount of vitamins while improving diet. The medicine treatment mainly comprises anti-inflammatory, liver protecting and anti-fibrosis medicines. At present, modern medicine mainly aims at alcohol rehabilitation and symptomatic treatment on alcoholic liver injury, and medicines which have definite curative effects and can be recommended for alcoholic liver injury are not yet available.
The traditional Chinese medicine has unique advantages in the aspect of treating the alcoholic liver disease, can be used for treating the alcoholic liver disease by differentiating symptoms and stages, or has good effect in treating the alcoholic liver disease through proved formulas and self-fitting formulas. At present, the traditional Chinese medicine for treating alcoholic liver injury mainly focuses on drugs for activating blood and dissolving stasis, promoting diuresis and removing dampness, relieving exterior syndrome and clearing heat, regulating qi and tonifying qi, and dispelling alcohol, and most of prescription drugs are mainly used for dispelling alcohol, or are biased to protect liver, and the two are very long. Most of the anti-hangover medicines are formed by simply combining a plurality of traditional Chinese medicines with anti-hangover effect, and the theoretical support of the traditional Chinese medicine is weak; the liver-protecting traditional Chinese medicine composition mainly aims at treating alcoholic liver injury at a certain stage and a certain syndrome type according to the stage and the type theory. Proved by experiments, the self-fitting prescription such as a prescription for clearing away stasis and toxic materials, a prescription for clearing liver and activating blood and the like mainly has the effects of clearing away heat and removing stasis; the soup for activating and nourishing the spleen is mainly used for tonifying the liver and the spleen; the soup mainly has the functions of clearing heat and detoxicating, eliminating phlegm and removing blood stasis, but has different advantages, but has no function of dispelling the effects of alcohol and protecting liver.
Therefore, the traditional Chinese medicine composition with definite curative effect, simple ingredients, and excellent curative effect and capabilities of dispelling the effects of alcohol and protecting liver is not known in the art.
Disclosure of Invention
Based on the above, the invention aims to provide the traditional Chinese medicine composition for preventing and treating alcoholic liver injury, which has simple raw material composition and definite curative effect.
In order to achieve the above purpose, the present invention adopts the following technical scheme.
The invention provides a traditional Chinese medicine composition for preventing and treating alcoholic liver injury, which is prepared from the following raw materials in parts by weight: 20-40 parts of kudzuvine root, 10-25 parts of hovenia dulcis thunb, 3-15 parts of bupleurum, and 5-20 parts of red paeony root.
In some embodiments, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 25-35 parts of kudzuvine root, 10-20 parts of hovenia dulcis thunb, 5-12 parts of bupleurum, and 5-15 parts of red paeony root.
In some preferred embodiments, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 28-32 parts of kudzuvine root, 13-17 parts of hovenia dulcis thunb, 5-10 parts of bupleurum, and 8-12 parts of red paeony root.
More preferably, the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 30 parts of kudzuvine root, 15 parts of hovenia dulcis thunb, 8 parts of bupleurum, and 10 parts of red paeony root.
In a second aspect of the present invention, there is provided a method for preparing the above-described Chinese medicinal composition, comprising the steps of: weighing radix Puerariae, semen Hoveniae, bupleuri radix and radix Paeoniae Rubra in proportion, extracting with extraction solvent, collecting extractive solution, and concentrating.
In some embodiments, the extraction solvent is water.
In some embodiments, the extraction is a decoction extraction.
In a third aspect of the present invention, there is provided a medicament for preventing and treating alcoholic liver injury, the medicament comprising a Chinese medicinal composition as described above.
In some embodiments, the medicament further comprises a pharmaceutically acceptable adjuvant.
In some embodiments, the dosage form of the medicament comprises granules, oral liquid, pills, tablets, capsules, decoction, and ointment.
In a fourth aspect of the present invention, there is provided a method for preparing a medicament as described above, the medicament being in the form of granules or pellets, comprising the steps of: concentrating the Chinese medicinal composition into extract, pulverizing, adding adjuvants, and making into corresponding dosage forms.
In some embodiments, the medicament is a granule, and the method of preparing the medicament comprises the steps of: drying the traditional Chinese medicine composition, crushing, taking dry extract powder, uniformly mixing with sugar powder and dextrin, drying to remove a small amount of water, adding ethanol to prepare a soft material, preparing wet granules by using an extrusion granulator, and drying and granulating to obtain granules.
In some embodiments, the medicament is a pill, the method of making which comprises the steps of: drying the Chinese medicinal composition, pulverizing into the finest powder, making into micropill with ethanol as wetting agent, sieving, and drying to obtain pill.
The invention obtains the traditional Chinese medicine composition for preventing and treating alcoholic liver injury with simple composition and definite curative effect through optimization, and the active ingredients of the traditional Chinese medicine composition are prepared from the following 4 raw materials with proper content: radix Puerariae, semen Hoveniae, bupleuri radix and radix Paeoniae Rubra. In the formula, kudzuvine root is used as a monarch drug for sweating and relieving muscles, relieving exterior syndrome and promoting eruption, ascending yang and relieving diarrhea, relieving restlessness and quenching thirst; hovenia dulcis thunb is used as ministerial drug for clearing heat and relieving restlessness, promoting fluid production, relieving urination and defecation and relieving alcoholism; red paeony root, radix paeoniae rubra, which is used as an adjuvant drug for clearing heat and cooling blood, removing blood stasis and relieving pain; bupleurum root, radix bupleuri, having the effects of relieving exterior syndrome and heat, soothing liver and relieving depression, lifting yang qi, and guiding all the drugs into the liver meridian, is an adjuvant drug. The four medicines are combined together to play roles of dispelling the effects of alcohol, relieving restlessness, clearing heat, promoting diuresis, soothing liver and activating blood, and simultaneously has the effects of dispelling the effects of alcohol and protecting liver, and is used for treating acute alcoholism and chronic alcoholic liver injury. The inventor summarizes clinical medication experience according to the compatibility principle of monarch, minister, assistant and guide, has reasonable and concise formula and uniqueness, and fully plays the characteristics of simplicity, convenience, cheapness and inspection of traditional Chinese medicine. The 4 raw materials are mutually matched and complement each other, so that the efficacy of the raw materials is maximized under the condition of simple formula, and the traditional Chinese medicine composition has no toxic or side effect. The composition can be found to be effective in improving symptoms of a drinker during clinical application, such as: dizziness, headache, nausea, etc., and has obvious improving effect on alcoholic fatty liver and alcoholic hepatitis.
Drawings
FIG. 1 shows the results of TG and TC content detection in each group of mice.
FIG. 2 shows the results of ALT and AST levels in mice of each group.
FIG. 3 shows the results of HE staining of liver of mice in each group.
FIG. 4 shows the results of liver oil red O staining of mice in each group.
FIG. 5 shows the liver sirius red staining results for each group of mice.
Detailed Description
The experimental procedure of the present invention, in which no specific conditions are noted in the following examples, is generally followed by routine conditions, such as, for example, sambrook et al, molecular cloning: conditions described in the laboratory Manual (New York: cold Spring Harbor Laboratory Press, 1989) or as recommended by the manufacturer. The various chemicals commonly used in the examples are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
The terms "comprising" and "having" and any variations thereof, are intended to cover a non-exclusive inclusion. For example, a process, method, apparatus, article, or device that comprises a list of steps is not limited to the elements or modules listed but may alternatively include additional steps not listed or inherent to such process, method, article, or device.
The following describes the embodiments in detail.
Example 1
Raw materials: 30 g of kudzuvine root, 15 g of hovenia dulcis thunb, 8g of bupleurum, and 10 g of red paeony root.
The steps are as follows: pulverizing the medicinal materials, placing into a proper decoction container, adding 10 times of water to submerge the medicinal materials, soaking for proper time, heating to boil, decocting for 30min for the first time, decocting for 20min for the second time, separating decoction, decocting medicinal residues for 2-3 times, collecting decoction, mixing decoctions, settling, filtering, concentrating at low temperature, and drying.
Mixing the dry extract powder with sugar powder, dextrin, and hot air drying at 80deg.C to remove small amount of water, making into soft material with 90% ethanol, making into wet granule with extrusion granulator, and drying to obtain granule.
Example 2
Raw materials: 40 g of kudzuvine root, 20 g of hovenia dulcis thunb, 10 g of bupleurum, and 15 g of red paeony root.
The steps are as follows: pulverizing the medicinal materials, placing into a proper decoction container, adding 10 times of water to submerge the medicinal materials, soaking for proper time, heating to boil, decocting for 30 min for the first time, decocting for 20 min for the second time, separating decoction, decocting medicinal residues for 2-3 times, collecting decoction, mixing decoctions, adding appropriate amount of 95% ethanol, monosaccharide slurry and sodium benzoate, filtering, and concentrating to obtain oral liquid.
Example 3
Raw materials: radix Puerariae 20 g, semen Hoveniae 10 g, bupleuri radix 5 g, radix Paeoniae Rubra 5 g.
The steps are as follows: pulverizing the medicinal materials, placing into a proper decoction container, adding 10 times of water to submerge the medicinal materials, soaking for proper time, heating to boil, decocting for 30min for the first time, decocting for 20min for the second time, separating decoction, decocting medicinal residues for 2-3 times, collecting decoction, mixing decoctions, settling, filtering, concentrating at low temperature, and drying.
Pulverizing the dry extract into the finest powder, making into micropill with ethanol as wetting agent, sieving, and drying at 60deg.C to obtain pill.
Example 4
In vitro efficacy test
1 Material
1.1 Animals: a total of 36C 57 mice.
1.2 Drugs and reagents: the Chinese medicinal composition granule (hereinafter referred to as honeydew melon soup) in example 1 has silymarin as positive medicinal component.
2 Method
2.1 The C57 mice were randomly divided into four groups, control group, model group, positive drug group, melon soup group. The three groups, except the control group, established a mouse model of alcoholic liver disease. The alcohol concentration was gradually increased from 1% to 5% for the first week of feeding, 2% every two days, 5% was maintained and feeding continued for 4 weeks. After 4 weeks, the model group, the positive medicine group and the melon soup group are respectively treated as follows: the model group irrigates the stomach with physiological saline at a dose of 60mg/kg per day; the positive medicine group is subjected to gastric lavage according to 50mg/kg per day; the honeydew melon soup group is subjected to stomach irrigation according to 60mg/kg per day. While the stomach was being irrigated, each group was fed with either alcohol feed or control feed.
2.2 Detection: detecting the serum ASL, ALT, TG, TC content of the mice after 4 weeks of gastric lavage; mouse liver oil red O staining, HE staining, sirius red staining.
3 Results
1 Triglyceride (TG) and Total Cholesterol (TC) content
As shown in FIG. 1, the positive drug group and the melon soup group have significantly reduced TG and TC contents compared with the model group, and the difference has statistical significance (P < 0.05).
2 Glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) content
As shown in FIG. 2, the positive drug group and the melon soup group showed significantly lower ALT and AST contents than the model group, and the difference was statistically significant (P < 0.05).
3 Liver HE staining
Liver HE staining suggests that the liver tissue of the mice in the model group has remarkable fatty degeneration and inflammation degree, and the melon soup intervention can obviously reduce the fatty degeneration and inflammation degree of the liver tissue and improve the morphology and structure of liver cells (figure 3).
4 Liver visceral oil red O staining
Liver oil red O staining suggests that the liver tissue lipid deposition of mice in the model group is remarkable, and the liver tissue lipid deposition degree is reduced after the intervention of the honeydew melon soup is adopted (figure 4).
5 Liver Tilletia red dyeing
Liver sirius red staining suggests that the liver tissue fibrosis degree of mice in the model group is obviously higher than that of mice in the control group, the positive medicine group and the honeydew group, and the liver fibrosis degree of the mice is effectively relieved after the intervention of the honeydew (figure 5).
In conclusion, the composition provided by the invention has an obvious liver protection effect under the condition of only 4 raw materials, and can effectively reduce liver lipid deposition and liver fibrosis.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (10)
1. The traditional Chinese medicine composition for preventing and treating alcoholic liver injury is characterized by comprising the following active ingredients in parts by weight: 20-40 parts of kudzuvine root, 10-25 parts of hovenia dulcis thunb, 3-15 parts of bupleurum, and 5-20 parts of red paeony root.
2. The traditional Chinese medicine composition according to claim 1, wherein the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 25-35 parts of kudzuvine root, 10-20 parts of hovenia dulcis thunb, 5-12 parts of bupleurum, and 5-15 parts of red paeony root.
3. The traditional Chinese medicine composition according to claim 2, wherein the active ingredients of the traditional Chinese medicine composition are prepared from the following raw materials in parts by weight: 28-32 parts of kudzuvine root, 13-17 parts of hovenia dulcis thunb, 5-10 parts of bupleurum, and 8-12 parts of red paeony root.
4. The method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 3, comprising the following steps: weighing radix Puerariae, semen Hoveniae, bupleuri radix and radix Paeoniae Rubra in proportion, extracting with extraction solvent, collecting extractive solution, and concentrating.
5. The method according to claim 4, wherein the extraction solvent is water.
6. The method of claim 4, wherein the extraction is a decoction extraction.
7. A medicament for preventing and treating alcoholic liver injury, which is characterized by comprising the traditional Chinese medicine composition according to any one of claims 1-3.
8. The medicament of claim 7, further comprising pharmaceutically acceptable excipients.
9. The medicament according to claim 7, wherein the dosage form of the medicament comprises granules, oral liquid, pills, tablets, capsules, decoction and ointment.
10. A method of preparing a medicament according to any one of claims 7 to 9, wherein the medicament is in the form of granules or pellets comprising the steps of: concentrating the Chinese medicinal composition into extract, pulverizing, adding adjuvants, and making into corresponding dosage forms.
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