CN117815151A - Sugar-resistant and antioxidant composition and preparation method and application thereof - Google Patents
Sugar-resistant and antioxidant composition and preparation method and application thereof Download PDFInfo
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- CN117815151A CN117815151A CN202410006556.5A CN202410006556A CN117815151A CN 117815151 A CN117815151 A CN 117815151A CN 202410006556 A CN202410006556 A CN 202410006556A CN 117815151 A CN117815151 A CN 117815151A
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- sugar
- parts
- leaves
- composition
- oxygen
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- BIVBRWYINDPWKA-VLQRKCJKSA-L Glycyrrhizinate dipotassium Chemical compound [K+].[K+].O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@H]1CC[C@]2(C)[C@H]3C(=O)C=C4[C@@H]5C[C@](C)(CC[C@@]5(CC[C@@]4(C)[C@]3(C)CC[C@H]2C1(C)C)C)C(O)=O)C([O-])=O)[C@@H]1O[C@H](C([O-])=O)[C@@H](O)[C@H](O)[C@H]1O BIVBRWYINDPWKA-VLQRKCJKSA-L 0.000 claims description 6
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/58—Meliaceae (Chinaberry or Mahogany family), e.g. Azadirachta (neem)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
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- A—HUMAN NECESSITIES
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
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- Chemical & Material Sciences (AREA)
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- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
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- Dermatology (AREA)
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- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Birds (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention provides an anti-sugar and anti-oxygen composition, and a preparation method and application thereof, and belongs to the technical field of plant extraction. The sugar-resistant and antioxidant composition comprises 10-40 parts of bilberry leaves, 5-20 parts of neem leaves and 20-60 parts of cymbidium hance roots, wherein various bioactive components in the composition can obviously inhibit the generation of AGEs (glycation end products) through reasonable compatibility and synergy so as to reduce the damage of saccharification to skin and promote the generation and repair of collagen; obviously eliminates superoxide radicals and hydroxyl radicals, prevents oxidation reaction, and protects cells from oxidative damage; meanwhile, the effects of moisturizing and relieving can be achieved. The product (such as the sugar and oxygen resisting essence) prepared by the composition has stable property, no special smell, good skin feel when in use, remarkable sugar and oxygen resisting effect and capability of remarkably improving and delaying skin aging.
Description
Technical Field
The invention belongs to the technical field of plant extraction, and particularly relates to an anti-sugar and antioxidant composition, and a preparation method and application thereof.
Background
Saccharification and oxidative damage are the main causes of aging skin lesions. Saccharification is a chemical reaction of glycosylation end products (AGEs) generated by the combination of sugar and protein in the body, and AGEs can influence the stable state of skin cells and the protein structure, so that collagen in the skin loses elasticity and is yellowing, loose, fragile and the like. Oxidation is mostly caused by free radicals, which are very active, and because of their lack of electrons, they abstract electrons from normal tissue structures, which are destroyed, and the main subjects of free radical attack are mainly DNA and enzymes in cells. Skin oxidation can cause problems such as dark yellow complexion, uneven complexion, dry skin, and obvious fine lines and spots. The higher level of free radicals is helpful for forming AGEs, and after the receptor (AGER) of the AGEs is activated, the body can be induced to generate a large amount of free radicals, so that the burden of an in-vivo antioxidant system is increased, skin tissues are further damaged, melanin is stimulated to generate, the skin is trapped in malignant circulation, and skin aging is accelerated.
Saccharification and oxidation are two important causes of skin aging, so that the saccharification and oxidation are very interesting research hotspots in the field of anti-aging cosmetics at present, few anti-aging products with synergistic effect through an anti-sugar and anti-oxygen dual-path are on the market at present, meanwhile, along with the improvement of the living standard of people, the saccharification and oxidation are more interesting in the market for the raw materials with the effects of being derived from pure natural and green, and therefore, the development of the raw materials with the effects of resisting sugar and oxygen from plants is an important research direction in the cosmetic industry at present.
Disclosure of Invention
Therefore, the invention aims to provide an anti-sugar and anti-oxygen composition, a preparation method and application thereof, wherein the composition can not only resist free radicals generated by an organism, but also prevent the generation of AGEs (advanced glycation end products), and the skin aging is delayed and the healthy and young state of the skin is maintained through bidirectional regulation of anti-sugar and anti-oxygen.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides an anti-sugar and anti-oxygen composition which comprises the following raw materials in parts by weight: 10-40 parts of bilberry leaves, 5-20 parts of neem leaves and 20-60 parts of cymbidium hancei roots.
Preferably, the material comprises the following raw materials in parts by weight: 15-30 parts of bilberry leaves, 6-15 parts of neem leaves and 35-55 parts of cymbidium hancei root.
Preferably, the material comprises the following raw materials in parts by weight: 24 parts of bilberry leaves, 8 parts of neem leaves and 48 parts of cymbidium hancei.
The invention also provides a preparation method of the sugar-resistant and antioxidant composition, which comprises the following steps:
pulverizing Vaccinium myrtillus L.leaf, neem leaf and radix Isatidis, mixing to obtain mixed powder, mixing with water, ultrasonic extracting, filtering, and collecting filtrate to obtain the final product.
Preferably, the particle size of the crushed particles is 10-100 meshes; the mass volume ratio of the mixed powder to the water is 1:5-30 g/mL; the power of the ultrasonic wave is 200-1000W, the temperature of the ultrasonic wave is 40-80 ℃, the times of ultrasonic wave extraction are 1-3 times, and the time of each ultrasonic wave extraction is 10-60 min.
The invention also provides an anti-sugar and anti-oxygen composition obtained by the preparation method.
The invention also provides application of the sugar-resistant and antioxidant composition in preparation of anti-aging products.
Preferably, the mass percentage of the sugar-resistant and antioxidant composition in the product is 0.1-2%; the product comprises a pharmaceutical or cosmetic product; the cosmetic comprises a toning lotion, an essence, an emulsion, a cream, a gel, a facial mask or a facial cleanser.
The invention also provides an anti-sugar and anti-oxygen essence which comprises 0.1-2% of the anti-sugar and anti-oxygen composition in percentage by mass.
Preferably, the anti-sugar and anti-oxidation essence also comprises, by mass, 2% -4% of butanediol, 2% -4% of glycerol, 0.2% -0.4% of carboxymethyl chitosan, 0.2% -0.6% of sodium hyaluronate, 0.05% -0.15% of ethylenediamine tetraacetic acid sodium, 0.2% -1.0% of polyethylene glycol/PPG-17/6 copolymer, 0.05% -0.15% of dipotassium glycyrrhizinate, 1% -3% of butanediol, 0.5% -0.7% of phenoxyethanol, 0.02% -0.07% of rose essential oil and the balance of water.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides an anti-sugar and anti-oxygen composition and a preparation method and application thereof, the anti-sugar and anti-oxygen composition is a natural active component, the bilberry leaf extract prepared by the preparation method is rich in flavonoids and anthocyanin compounds, the neem leaf extract is rich in flavonoids and terpenoids, the hanlan root extract is rich in polysaccharide components, and various bioactive components in the composition can obviously inhibit the generation of glycosylation end products AGEs through reasonable compatibility and synergy, so as to reduce the damage of saccharification to skin and promote the generation and repair of collagen; obviously eliminates superoxide radicals and hydroxyl radicals, prevents oxidation reaction, and protects cells from oxidative damage; meanwhile, the effects of moisturizing and relieving can be achieved. The product (such as the sugar and oxygen resisting essence) prepared by the composition has stable property, no special smell, good skin feel when in use, remarkable sugar and oxygen resisting effect and capability of remarkably improving and delaying skin aging.
Drawings
FIG. 1 shows the results of the variation of skin moisture content of subjects with days of use after the use of different essences.
Detailed Description
The invention provides an anti-sugar and anti-oxygen composition which comprises the following raw materials in parts by weight: 10-40 parts of bilberry leaves, 5-20 parts of neem leaves and 20-60 parts of cymbidium hancei roots.
In the invention, the bilberry (Vaccinium myrtillus Linn) is a bilberry leaf shrub of the genus Vaccinium of the family Ericaceae, and the bilberry leaf extract is rich in flavonoids and anthocyanin compounds; said neem (Azadirachta indica) is deciduous tree of Garlic genus of Meliaceae family, and neem leaf extract is rich in flavonoids and terpenoids; the cymbidium kanran (Cymbidium kanran Makino) is a terrestrial herbal plant of cymbidium of Orchidaceae, the extract of cymbidium kanran is rich in polysaccharide components, and the cymbidium kanran is cymbidium kanran. The invention reasonably combines the bilberry leaves, the bilberry leaves and the cymbidium kanran, and the bilberry leaves, the cymbidium kanran and the cymbidium kanran complement each other, and the combination of the components has obvious sugar and oxygen resisting effects. The material comprises the following raw materials in parts by weight: 15-30 parts of bilberry leaves, 6-15 parts of neem leaves and 35-55 parts of cymbidium hancei roots. Further preferably, the feed comprises the following raw materials in parts by weight: 24 parts of bilberry leaves, 8 parts of neem leaves and 48 parts of cymbidium hancei. As a preferred embodiment, the sugar-resistant and oxygen-resistant composition is composed of the following raw materials in parts by weight: 10-40 parts of bilberry leaves, 5-20 parts of neem leaves and 20-60 parts of cymbidium hancei roots; it is further preferable that the composition comprises the following raw materials in parts by weight: 15-30 parts of bilberry leaves, 6-15 parts of neem leaves and 35-55 parts of cymbidium hancei roots; more preferably, the material comprises the following raw materials in parts by weight: 24 parts of bilberry leaves, 8 parts of neem leaves and 48 parts of cymbidium hancei.
The invention also provides a preparation method of the sugar-resistant and antioxidant composition, which comprises the following steps:
pulverizing Vaccinium myrtillus L.leaf, neem leaf and radix Isatidis, mixing to obtain mixed powder, mixing with water, ultrasonic extracting, filtering, and collecting filtrate to obtain the final product.
The invention pulverizes the bilberry leaf, the neem leaf and the hanlan root, and the mixture is evenly mixed to obtain mixed powder. The particle size of the pulverized particles is preferably 10 to 100 mesh, more preferably 20 to 80 mesh. After the mixed powder is obtained, the mixed powder is mixed with water, ultrasonic extraction is carried out, filtration is carried out, and filtrate is collected, so that the sugar-resistant and oxygen-resistant composition is obtained. The mass volume ratio of the mixed powder to water is preferably 1:5-30 g/mL, and more preferably 1:8-20 g/mL. The power of the ultrasound is preferably 200 to 1000W, more preferably 400 to 800W, the temperature of the ultrasound is preferably 40 to 80 ℃, more preferably 50 to 60 ℃, the number of times of ultrasound extraction is preferably 1 to 3, more preferably 2, and the time of each ultrasound extraction is preferably 10 to 60min, more preferably 20 to 40min. The preparation method further comprises the steps of concentrating the filtrate, and drying, preferably freeze drying, the filtrate, wherein the concentration is reduced pressure concentration. The invention selects the mass volume ratio of the crushed particle size, the mixed powder and the water and the ultrasonic extraction condition, can effectively promote the dissolution of active ingredients in bilberry leaves, neem leaves and hanlan roots, and the combined action of the active ingredients synergistically increases the synergistic effect, generates obvious sugar and oxygen resisting effects, and obviously improves and delays skin aging.
The invention also provides an anti-sugar and anti-oxygen composition obtained by the preparation method.
The invention also provides application of the sugar-resistant and antioxidant composition in preparation of anti-aging products.
In the invention, the mass percentage of the sugar-resistant and oxygen-resistant composition in the product is preferably 0.1-2%. The product preferably comprises a pharmaceutical or cosmetic product; the cosmetic comprises a toning lotion, an essence, an emulsion, a cream, a gel, a facial mask or a facial cleanser.
The invention also provides an anti-sugar and anti-oxygen essence which comprises 0.1-2% of the anti-sugar and anti-oxygen composition in percentage by mass.
In the invention, the anti-sugar and anti-oxidation essence preferably further comprises, by mass, 2-4% of butanediol, 2-4% of glycerol, 0.2-0.4% of carboxymethyl chitosan, 0.2-0.6% of sodium hyaluronate, 0.05-0.15% of sodium ethylenediamine tetraacetate, 0.2-1.0% of polyethylene glycol/PPG-17/6 copolymer, 0.05-0.15% of dipotassium glycyrrhizinate, 1-3% of butanediol, 0.5-0.7% of phenoxyethanol, 0.02-0.07% of rose essential oil and the balance of water. Without any particular explanation, the sources of other components of the anti-sugar antioxidant essence of the present invention are not particularly limited, and products commercially available in the art or products prepared by conventional methods may be used.
The preparation method of the sugar-resistant and antioxidant essence comprises the following steps: dispersing carboxymethyl chitosan and sodium hyaluronate uniformly by using butanediol and glycerol, adding water, heating to 55-65 ℃, stirring, cooling to 40-50 ℃, sequentially adding components of sodium ethylenediamine tetraacetate, polyethylene glycol/PPG-17/6 copolymer, dipotassium glycyrrhizinate, butanediol, an anti-sugar antioxidant composition, phenoxyethanol and rose essential oil, and stirring uniformly to obtain the product. The sugar and oxygen resisting essence has long-acting moisturizing effect, and simultaneously has the functions of smoothing skin and improving wrinkles, so that the sugar and oxygen resisting essence has remarkable anti-aging effect.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
An anti-sugar and anti-oxygen composition comprises the following raw materials:
bilberry leaf 15g, neem leaf 15g and cymbidium kanran 35g.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 15g of bilberry leaves, 15g of neem leaves and 35g of han lan Gen, crushing to 10 meshes by a crusher, uniformly mixing to obtain mixed powder, adding 1300mL of water into the mixed powder, carrying out ultrasonic extraction for 60min at 40 ℃ under the ultrasonic power of 1000W, extracting for 1 time, filtering, collecting filtrate, concentrating the filtrate under reduced pressure, and freeze-drying to obtain the anti-sugar and anti-oxygen composition.
Example 2
An anti-sugar and anti-oxygen composition comprises the following raw materials:
bilberry leaf 30g, neem leaf 15g and cymbidium kanran 35g.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 30g of bilberry leaves, 15g of neem leaves and 35g of han lan Gen, crushing the bilberry leaves, the neem leaves and the han lan Gen to 100 meshes by using a crusher, uniformly mixing the crushed bilberry leaves, the neem leaves, the han Lian Gen and the han Lian Gen, adding 2400mL of water into the mixed powder, carrying out ultrasonic extraction for 20min at 60 ℃ under the condition of ultrasonic power of 800W, filtering and collecting filtrate a, carrying out ultrasonic extraction for 20min on filter residues at 60 ℃ under the condition of ultrasonic power of 800W, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the anti-sugar and anti-oxygen composition.
Example 3
An anti-sugar and anti-oxygen composition comprises the following raw materials:
bilberry leaf 24g, neem leaf 8g and cymbidium kanran root 48g.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 24g of bilberry leaves, 8g of neem leaves and 48g of han lan Gen, crushing the bilberry leaves, the neem leaves and the han lan Gen to 30 meshes by using a crusher, uniformly mixing the crushed bilberry leaves, the neem leaves, the han lan Gen and the han lan Gen to obtain mixed powder, adding 800mL of water into the mixed powder, carrying out ultrasonic extraction for 30min under the condition of 55 ℃ and ultrasonic power 600W, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues under the condition of 55 ℃ and ultrasonic power 600W for 30min, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the anti-sugar and anti-oxygen composition.
Example 4
An anti-sugar and anti-oxygen composition comprises the following raw materials:
bilberry leaf 15g, neem leaf 6g and cymbidium kanran 55g.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 15g of bilberry leaves, 6g of neem leaves and 55g of han lan Gen, crushing the bilberry leaves, the neem leaves and the han lan Gen into 80 meshes by a crusher, uniformly mixing the crushed bilberry leaves, the neem leaves, the han Lian Gen and the han Lian, adding 380mL of water into the mixed powder, carrying out ultrasonic extraction for 10min under the conditions of 80 ℃ and 200W of ultrasonic power, filtering and collecting filtrate a and filter residue a, carrying out ultrasonic extraction for 10min under the conditions of 80 ℃ and 200W of ultrasonic power on the filter residue a, filtering and collecting filtrate b and filter residue b, carrying out ultrasonic extraction for 10min under the conditions of 80 ℃ and 200W of ultrasonic power on the filter residue b, filtering and collecting filtrate c, combining the filtrate a, the filtrate b and the filtrate c to obtain filtrate d, carrying out reduced pressure concentration on the filtrate d, and freeze drying to obtain the anti-sugar and anti-oxygen composition.
Example 5
An anti-sugar and anti-oxygen composition comprises the following raw materials:
bilberry leaf 10g, neem leaf 5g and cymbidium kanran root 20g.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 10g of bilberry leaves, 5g of neem leaves and 20g of han lan Gen, crushing the bilberry leaves, the neem leaves and the han lan Gen to 30 meshes by using a crusher, uniformly mixing the crushed bilberry leaves, the neem leaves, the han Lian Gen and the han Lian, adding 350mL of water into the mixed powder, carrying out ultrasonic extraction for 30min under the conditions of 55 ℃ and 600W of ultrasonic power, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues under the conditions of 55 ℃ and 600W of ultrasonic power for 30min, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the anti-sugar and anti-oxygen composition.
Example 6
An anti-sugar and anti-oxygen composition comprises the following raw materials:
40g of bilberry leaf, 20g of neem leaf and 60g of cymbidium hancei.
The preparation method of the sugar-resistant and antioxidant composition comprises the following steps:
weighing 40g of bilberry leaves, 20g of neem leaves and 60g of han lan Gen, crushing the bilberry leaves, the neem leaves and the han lan Gen to 30 meshes by using a crusher, uniformly mixing the crushed bilberry leaves, the neem leaves, the han Lian Gen and the han Lian, adding 1200mL of water into the mixed powder, performing ultrasonic extraction for 30min under the conditions of 55 ℃ and ultrasonic power of 600W, filtering and collecting filtrate a, performing ultrasonic extraction for 30min on filter residues under the conditions of 55 ℃ and ultrasonic power of 600W, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the anti-sugar and anti-oxygen composition.
Comparative example 1
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 24g of bilberry leaves and 8g of neem leaves, crushing the bilberry leaves and the neem leaves to 30 meshes by using a crusher, uniformly mixing the bilberry leaves and the neem leaves to obtain mixed powder, adding 320mL of water into the mixed powder, carrying out ultrasonic extraction for 30min under the condition of ultrasonic power of 600W at 55 ℃, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues for 30min under the condition of ultrasonic power of 600W at 55 ℃, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Comparative example 2
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 8g of neem leaves and 48g of han lan Gen, crushing the neem leaves and the han lan Gen to 30 meshes by a crusher, uniformly mixing the materials to obtain mixed powder, adding 560mL of water into the mixed powder, carrying out ultrasonic extraction for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues at 55 ℃ under the condition of ultrasonic power of 600W for 30min, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Comparative example 3
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 24g of bilberry leaves and 48g of han lan Gen, crushing the bilberry leaves and the han lan Gen to 30 meshes by using a crusher, uniformly mixing the bilberry leaves and the han lan Gen to obtain mixed powder, adding 720mL of water into the mixed powder, carrying out ultrasonic extraction for 30min under the condition of ultrasonic power of 600W at 55 ℃, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues under the condition of 55 ℃ and ultrasonic power of 600W for 30min, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Comparative example 4
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 50g of bilberry leaves, crushing the bilberry leaves to 30 meshes by using a crusher, uniformly mixing the bilberry leaves and the water to obtain mixed powder, adding 500mL of water into the mixed powder, carrying out ultrasonic extraction for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Comparative example 5
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 50g of neem leaves, crushing the neem leaves to 30 meshes by a crusher, uniformly mixing the neem leaves and the solution to obtain mixed powder, adding 500mL of water into the mixed powder, carrying out ultrasonic extraction for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate a, carrying out ultrasonic extraction on filter residues for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Comparative example 6
A preparation method of an anti-sugar and anti-oxygen composition comprises the following steps:
weighing 50g of cymbidium hance, crushing to 30 meshes by a crusher, uniformly mixing to obtain mixed powder, adding 500mL of water into the mixed powder, carrying out ultrasonic extraction for 30min at 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate a, carrying out ultrasonic extraction for 30min on filter residues under the condition of 55 ℃ under the condition of ultrasonic power of 600W, filtering and collecting filtrate b, combining the filtrate a and the filtrate b to obtain filtrate c, concentrating the filtrate c under reduced pressure, and freeze-drying to obtain the sugar-resistant and antioxidant composition.
Test example 1 in vitro inhibition of non-enzymatic glycosylation assay
(1) Solution preparation
1% sample solution: 1.00g of the sugar-resistant and oxygen-resistant composition prepared in examples 1-4 and comparative examples 1-6 are respectively weighed, 99.00g of deionized water is added, stirred and dissolved, and the filtrate is filtered, thus obtaining the sugar-resistant and oxygen-resistant composition.
Phosphate buffer (0.2 mol/L, pH 7.4): 71.64g of disodium hydrogen phosphate was dissolved in 1000ml of water to prepare a solution A; 31.21g of sodium dihydrogen phosphate was dissolved in 1000ml of water to prepare a solution B; mixing 810mLA solution and 190mLB solution, and adding 0.2% proclin 300 to obtain antibacterial phosphate buffer solution.
Bovine serum albumin solution (20 mg/mL): 4.0g of bovine serum albumin was weighed and dissolved in 200mL of phosphate buffer.
Glucose solution (200 mg/mL): 40.0g of glucose was weighed and dissolved in 200mL of phosphate buffer.
(2) Experimental operation
Each tube of the reagent was prepared as in Table 1, and incubated in a light-resistant incubator at 37℃for 10 days, and then detected under fluorescence conditions of excitation wavelength of 370nm and emission wavelength of 440 nm. Calculated as follows:
wherein: a is absorbance of the sample solution in the reaction system; b is absorbance in the reaction system replaced by phosphate buffer solution (bovine serum albumin solution+glucose solution); c is absorbance of the sample solution in the reaction system by using phosphate buffer solution instead; d is absorbance in the reaction system with phosphate buffer.
TABLE 1
The results of the non-enzymatic glycosylation end product AGEs inhibition test for the different treated samples are shown in table 2.
TABLE 2 results of non-enzymatic glycosylation end product AGEs inhibition test of different treated samples
As can be seen from the data in Table 2, the inhibition rates of the non-enzymatic glycosylation end products AGEs in examples 1 to 4 are all significantly higher than those in comparative examples 1 to 6, which indicates that the anti-glycation and anti-oxidation composition of the present invention has a significant anti-glycation effect by the synergistic effect of 3 components, and the absence of any one or only any one of the components significantly reduces the inhibition effects of the non-enzymatic glycosylation end products AGEs, thereby reducing the anti-glycation effect thereof.
Test example 2 antioxidation test
(1) Hydroxy radical scavenging rate (salicylic acid method)
(1) Solution preparation
1% sample solution: 1.00g of the sugar-resistant and oxygen-resistant composition prepared in examples 1-4 and comparative examples 1-6 are respectively weighed, 99.00g of deionized water is added, stirred and dissolved, and the filtrate is filtered, thus obtaining the sugar-resistant and oxygen-resistant composition.
9mmol/L salicylic acid solution: 0.1242g salicylic acid was dissolved in 100mL 95% ethanol.
9mmol/L FeSO 4 ·7H 2 O solution: 0.2502g FeSO 4 ·7H 2 O was dissolved in 100mL deionized water.
9mmol/L H 2 O 2 Solution: 0.1mL H 2 O 2 The solution was mixed well with 100mL deionized water (ready to use).
(2) Experimental operation
Sequentially adding the reagents into a cuvette according to Table 3, shaking, heating in a 37 ℃ water bath for 15min, taking out, and measuring the absorbance at 510nm on an ultraviolet-visible spectrophotometer.
Wherein: a is absorbance of distilled water in the reaction system instead of the sample solution; b is absorbance of the sample solution in the reaction system; c is absorbance of distilled water added into the system instead of hydrogen peroxide solution.
TABLE 3 Table 3
The results of the hydroxyl radical scavenging test for the different samples are shown in table 4.
TABLE 4 results of hydroxyl radical removal test for different samples
As can be seen from the data in Table 4, the hydroxyl radical scavenging rates of examples 1 to 4 are all significantly higher than those of comparative examples 1 to 6, which shows that the anti-sugar and anti-oxidant composition of the invention has a significant anti-oxidant effect by the synergistic effect of 3 components, and the absence of any one or only any one of the components significantly reduces the hydroxyl radical scavenging effect, thereby reducing the anti-oxidant effect.
(2) Superoxide anion scavenger (pyrogallol process)
(1) Solution preparation
1% sample solution: 1.00g of the sugar-resistant and oxygen-resistant composition prepared in examples 1-4 and comparative examples 1-6 are respectively weighed, 99.00g of deionized water is added, stirred and dissolved, and the filtrate is filtered, thus obtaining the sugar-resistant and oxygen-resistant composition.
0.1M Tris solution: 6.05g Tris (Tris) was weighed and the volume was fixed with distilled water to 500mL.
0.05M HCl solution: 4.29mL of concentrated hydrochloric acid (36% -38%) is measured, and distilled water is used for constant volume to 1000mL.
10mM Tris-HCl solution (pH 8.2): the pH was adjusted to 8.2 with 0.1M Tris solution and 0.05M HCl solution, respectively.
10mM HCl solution: the 0.05M HCl solution was diluted 5-fold with distilled water.
3mM o-trimellitic acid solution: 0.0378g of pyrogallol was taken up to 100mL (as prepared, 4h active) with 10mM HCl solution.
(2) Experimental operation
0.45mL of 10mM Tris-HCl solution, pH 8.2, was first incubated in a 25℃water bath for 20min. Respectively adding 0.1mL of sample solution and 0.04mL of 3mM o-phloroglucinol solution, uniformly mixing, reacting in a water bath at 25 ℃ for 5min, adding 0.1mL of 10mM HCl solution to stop the reaction, shaking uniformly, standing for 3min, and measuring the absorbance at the wavelength of 325 nm. Superoxide anion clearance was calculated according to the following formula. VC was used as a control.
Wherein: a is that i The absorbance of the solution to be measured in the reaction system; a is that j For the sample solution contained in the reaction system, 0.04mL of distilled water is used for replacing the absorbance of the pyrogallol solution added into the system; a is that 0 The absorbance of the sample solution in the reaction system was replaced with distilled water.
The results of the superoxide anion radical scavenging rate test for the different samples are shown in Table 5.
TABLE 5 results of test for superoxide anion radical scavenging from different samples
As can be seen from the data in Table 5, the scavenging rate of the superoxide anions in examples 1 to 4 is significantly higher than that in comparative examples 1 to 6, which shows that the antioxidant effect is significant by the synergistic effect of 3 components in the antioxidant composition of the invention, and the scavenging effect of the superoxide anions is significantly reduced by the absence of any one or only any one of the components, thereby reducing the antioxidant effect.
Application example 1 preparation of sugar-resistant antioxidant essence
The sugar-resistant and antioxidant essence consists of the following components in percentage by mass: phase A: 3% of butanediol, 3% of glycerol, 0.3% of carboxymethyl chitosan, 0.4% of sodium hyaluronate and 88.95% of water; and B phase: 0.1% of sodium ethylenediamine tetraacetate, 0.5% of polyethylene glycol/PPG-17/6 copolymer, 0.1% of dipotassium glycyrrhizinate and 1% of the anti-sugar and anti-oxygen composition of example 3; and C phase: 2% of butanediol and 0.6% of phenoxyethanol; and D phase: rose essential oil 0.05%.
The preparation method of the anti-sugar and anti-oxygen essence comprises the following steps: dispersing carboxymethyl chitosan and sodium hyaluronate with butanediol and glycerol, adding water, heating to 60deg.C, stirring to transparent to obtain phase A, cooling to 45deg.C, sequentially adding phase B, phase C and phase D, and stirring.
Preparation of blank essence using comparative example 1
A blank essence comprises the following components in percentage by weight: phase A: 3% of butanediol, 3% of glycerol, 0.3% of carboxymethyl chitosan, 0.4% of sodium hyaluronate and 89.95% of water; and B phase: 0.1% of sodium ethylenediamine tetraacetate, 0.5% of polyethylene glycol/PPG-17/6 copolymer and 0.1% of dipotassium glycyrrhizinate; and C phase: 2% of butanediol and 0.6% of phenoxyethanol; and D phase: rose essential oil 0.05%.
The preparation method of the blank essence comprises the following steps: dispersing carboxymethyl chitosan and sodium hyaluronate with butanediol and glycerol, adding water, heating to 60deg.C, stirring to transparent to obtain phase A, cooling to 45deg.C, sequentially adding phase B, phase C and phase D, and stirring.
Test example 3 human body test moisturizing efficacy
The subject: 16 healthy volunteers aged 18-50 years, no history of skin disease and cosmetic allergy; the basal value of the moisture content of the skin stratum corneum in the facial test area is between 15 and 45. The subject had to clean the facial area with the specifically provided cleanser before testing, wait for at least 20 minutes in the test environment, and had to eat, drink water and conduct vigorous activities during the test. Divided into 2 groups of 8 persons each.
Test environment: temperature (20.+ -. 2) DEG C, relative humidity (50%.+ -. 10%).
The testing method comprises the following steps: after the face of the subject is cleaned by the special facial cleanser, the subject is balanced for 20 minutes in a test environment, and the moisture content of the skin is measured; sample area (sugar and oxygen resisting essence prepared by application example 1, i.e. test group) and blank control area (blank essence prepared by application comparative example 1The Huaye, a blank group) is distributed on the face area of the volunteer according to a random distribution table of test areas, each area is 3.0cm multiplied by 3.0cm, and the sample dosage is (2.0+/-0.1) mg/cm 2 . The moisture content of the skin horny layer was tested on days 0, 7, 14, 21, 28, 35, 42, 49 and 56 before the use of the product, and the test results are shown in fig. 1.
As can be seen from the trend of the moisture content data in fig. 1, the moisture content of the skin horny layer of the subject is remarkably improved after using the anti-sugar and anti-oxygen essence prepared by the present invention, and the moisture content value of the skin is increased from 37.3% to 61.2% after 56 days of use over time, and is increased by 64% compared with the moisture content before use. Whereas the moisture content value of the skin increased from 37.8% to 50% after 56 days of continuous use, only about 32.3% from the moisture content before non-use. The long-acting moisturizing effect of the sugar-resistant and antioxidant essence (application example 1) prepared by the invention is 2 times that of the blank essence (application comparative example 1).
Test example 4 evaluation of anti-aging Effect of human body test
Grouping: 40 healthy volunteers aged 30-60 years have different degrees of wrinkles on the face, no history of skin disease and no history of cosmetic allergy, and are divided into 2 groups of 20 people each. The test period was 60 days, the test group used the anti-sugar and anti-oxygen essence during the test period (application example 1), the control group used the blank essence during the test period (application comparative example 1), and no other cosmetics or medicines that could affect the test results were used.
The testing method comprises the following steps: the subjects apply the essence to the face, use the essence once in the morning and evening for 60 days, the subjects follow up the use for one time in the first 0 days and 60 days after the use, and the subjects self evaluate 3 indexes of moisturizing effect, skin smoothing effect and wrinkle improvement effect by adopting a questionnaire investigation mode, collect data and calculate the effective rate. The evaluation scale is: has obvious effect (++), good effect (++), general effect (+), and basically no effect (-).
The anti-aging test effect is shown in table 6.
TABLE 6 anti-aging test effect
Note that: self-assessment as (++) and (++) a number of subjects who are effective, effective rate = number of effective persons ≡20×100%.
As can be seen from the data in table 6, after 20 subjects in the test group continuously use the anti-sugar and anti-oxygen essence (application example 1) for 60 days, the effective rates of 3 indexes of moisturizing effect, skin smoothing effect and wrinkle improvement effect are 75.0%, 80.0% and 65.0%, respectively; after the control group 20 subjects continuously use the blank essence (comparative example 1 was applied) for 60 days, the effective rates of 3 indexes are 60.0%, 55.0% and 30.0%, respectively. The human body trial effect of the test group is higher than that of the control group, and the test group shows that the test subject has better effects in the aspects of moisturizing, smoothing skin, improving wrinkles and the like by using the essence added with the anti-sugar and anti-oxygen composition, so that the anti-sugar and anti-oxygen composition provided by the invention has obvious anti-aging effect on the skin through the anti-glycosylation and anti-oxidation double-passage effect.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The sugar-resistant and oxygen-resistant composition is characterized by comprising the following raw materials in parts by weight: 10-40 parts of bilberry leaves, 5-20 parts of neem leaves and 20-60 parts of cymbidium hancei roots.
2. The composition according to claim 1, characterized by comprising the following raw materials in parts by weight: 15-30 parts of bilberry leaves, 6-15 parts of neem leaves and 35-55 parts of cymbidium hancei roots.
3. The composition according to claim 2, characterized by comprising the following raw materials in parts by weight: 24 parts of bilberry leaves, 8 parts of neem leaves and 48 parts of cymbidium hancei.
4. A process for the preparation of an anti-sugar and anti-oxidant composition according to any one of claims 1 to 3, comprising the steps of:
pulverizing Vaccinium myrtillus L.leaf, neem leaf and radix Isatidis, mixing to obtain mixed powder, mixing with water, ultrasonic extracting, filtering, and collecting filtrate to obtain the final product.
5. The method according to claim 4, wherein the pulverized particle size is 10 to 100 mesh; the mass volume ratio of the mixed powder to the water is 1:5-30 g/mL; the power of the ultrasonic wave is 200-1000W, the temperature of the ultrasonic wave is 40-80 ℃, the times of ultrasonic wave extraction are 1-3 times, and the time of each ultrasonic wave extraction is 10-60 min.
6. An anti-sugar and anti-oxidant composition obtained by the process of claim 4 or 5.
7. Use of the anti-sugar and anti-oxidant composition of claim 6 in the preparation of an anti-aging product.
8. The use according to claim 7, characterized in that the anti-sugar and anti-oxygen composition is present in the product in a mass percentage of 0.1% to 2%; the product comprises a pharmaceutical or cosmetic product; the cosmetic comprises a toning lotion, an essence, an emulsion, a cream, a gel, a facial mask or a facial cleanser.
9. An anti-sugar and anti-oxidation essence is characterized by comprising, by mass, 0.1% -2% of the anti-sugar and anti-oxidation composition according to claim 6.
10. The anti-wrinkle essence according to claim 9, further comprising, by mass, 2% -4% of butanediol, 2% -4% of glycerol, 0.2% -0.4% of carboxymethyl chitosan, 0.2% -0.6% of sodium hyaluronate, 0.05% -0.15% of ethylenediamine tetraacetic acid sodium, 0.2% -1.0% of polyethylene glycol/PPG-17/6 copolymer, 0.05% -0.15% of dipotassium glycyrrhizinate, 1% -3% of butanediol, 0.5% -0.7% of phenoxyethanol, 0.02% -0.07% of rose essential oil and the balance of water.
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