CN117769562A - 用于结合触发的抗体沉淀和纯化的高亲和力超分子聚合物 - Google Patents

用于结合触发的抗体沉淀和纯化的高亲和力超分子聚合物 Download PDF

Info

Publication number
CN117769562A
CN117769562A CN202280051543.9A CN202280051543A CN117769562A CN 117769562 A CN117769562 A CN 117769562A CN 202280051543 A CN202280051543 A CN 202280051543A CN 117769562 A CN117769562 A CN 117769562A
Authority
CN
China
Prior art keywords
molecules
protein
mab
ligand
precipitation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280051543.9A
Other languages
English (en)
Chinese (zh)
Inventor
崔宏刚
李奕
戴维·斯特恩
徐晅阔
陸丽琳
杰森·米尔斯
S·高斯
李正剑
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bristol Myers Squibb Co
Johns Hopkins University
Original Assignee
Bristol Myers Squibb Co
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co, Johns Hopkins University filed Critical Bristol Myers Squibb Co
Publication of CN117769562A publication Critical patent/CN117769562A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C07K17/02—Peptides being immobilised on, or in, an organic carrier
    • C07K17/08—Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
CN202280051543.9A 2021-07-20 2022-07-18 用于结合触发的抗体沉淀和纯化的高亲和力超分子聚合物 Pending CN117769562A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163223792P 2021-07-20 2021-07-20
US63/223,792 2021-07-20
PCT/US2022/073822 WO2023004273A1 (en) 2021-07-20 2022-07-18 High-affinity supramolecular polymers for binding-triggered antibody precipitation and purification

Publications (1)

Publication Number Publication Date
CN117769562A true CN117769562A (zh) 2024-03-26

Family

ID=84979716

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280051543.9A Pending CN117769562A (zh) 2021-07-20 2022-07-18 用于结合触发的抗体沉淀和纯化的高亲和力超分子聚合物

Country Status (6)

Country Link
US (1) US20240327455A1 (https=)
EP (1) EP4373839A4 (https=)
JP (1) JP2024526704A (https=)
KR (1) KR20240038740A (https=)
CN (1) CN117769562A (https=)
WO (1) WO2023004273A1 (https=)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1897887A1 (de) * 2006-09-08 2008-03-12 Universitätsklinikum Freiburg Split-Core-Partikel für die Präsentation von Fremdmolekülen, insbesondere für Impfstoffanwendungen und Verfahren zu deren Herstellung
WO2019036631A1 (en) * 2017-08-18 2019-02-21 The Johns Hopkins University SUPRAMOLECULAR FILAMENT ASSEMBLIES FOR THE PURIFICATION OF PROTEINS

Also Published As

Publication number Publication date
KR20240038740A (ko) 2024-03-25
JP2024526704A (ja) 2024-07-19
EP4373839A1 (en) 2024-05-29
WO2023004273A1 (en) 2023-01-26
US20240327455A1 (en) 2024-10-03
EP4373839A4 (en) 2025-12-17

Similar Documents

Publication Publication Date Title
JP7291395B2 (ja) ＩｇＧ結合ペプチド、及び該ペプチドによる抗体の特異的修飾
CN107614514B (zh) 利用IgG结合肽的抗体特异性修饰
JPWO2018199337A1 (ja) 可溶性タンパク質に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩
JP7794789B2 (ja) タンパク質精製のための超分子フィラメント集合体
JP2026053563A (ja) 補体関連疾患のための融合タンパク質構築物
JP7097293B2 (ja) ヒトＦｃ受容体に結合する融合タンパク質
TW202123970A (zh) 1價ccap生成物之製造方法
EP3597661A1 (en) New polypeptides
CN113365665A (zh) 抗Her2抗体药物偶联物药物制剂
JP2023011689A (ja) 生体高分子の精製のための超分子高アフィニティタンパク質結合系
CN117769562A (zh) 用于结合触发的抗体沉淀和纯化的高亲和力超分子聚合物
CN120209142A (zh) 一种靶向Ly6G6D的纳米抗体及其应用
TWI787151B (zh) 藉由ＩｇＧ結合肽之抗體之特異性裝飾
WO2026037338A1 (zh) 一种阻断baff、april作用其受体的融合蛋白
TW202611081A (zh) 一種阻斷ｂａｆｆ、ａｐｒｉｌ作用其受體的融合蛋白
WO2023169524A1 (zh) 白细胞介素2突变体以及含有其的复合物
HK40117415A (zh) 具有改善的稳定性的抗fcrn抗体或其抗原结合片段
TW202600604A (zh) Ghr激動型抗體及其應用
CN119343359A (zh) 多特异性抗体的纯化方法
HK40109611A (zh) 化合物、化合物的盐、抗体修饰试剂、修饰抗体的制造方法及修饰抗体
JP2020019723A (ja) 抗ＣＤ７０抗体とＩｇＧ結合ペプチドの複合体
CN116710138A (zh) 肽交联剂以及通过该交联剂交联的交联肽
HK40065797A (zh) 1价ccap生成物的制造方法
KR20130127366A (ko) ＩｇＧ형태의 ＶＥＧＦ에 결합하는 항체 및 ＤＬＬ4에 결합하는 단백질이 연결된 이중표적 단백질 및 이의 용도
최원우 Immunoglobulin G Fc-binding peptides and their applications

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination