CN117731759A - Mussel mucin and stem cell composition for growing and developing hair and preparation method thereof - Google Patents
Mussel mucin and stem cell composition for growing and developing hair and preparation method thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims description 11
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 12
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Abstract
The invention relates to the technical field of hair growth and development, in particular to a mussel mucin and stem cell composition for hair growth and development, which comprises the following components in parts by weight: 3-9 parts of mussel mucin, 1-3 parts of human hair follicle stem cells, 1-3 parts of human adipose mesenchymal stem cells, 1-3 parts of umbilical cord mesenchymal stem cells, 1-3 parts of stem cell factors, 15-22 parts of hair growth regulator, 10-18 parts of minoxidil, 6-16 parts of sodium hyaluronate and 30-40 parts of deionized water. According to the hair growth promoting agent, hair loss is treated by aiming at mussel mucin and stem cells, the hair growth promoting agent is prepared by adopting traditional Chinese medicines, the chemical components are few, the hair growth promoting effect is good, the human hair follicle stem cells, the human fat mesenchymal stem cells, the umbilical cord mesenchymal stem cells and stem cell factors are all extracted in human bodies, hair growth is carried out by regulating the stem cells at the hair loss position, the drug property absorption completeness is high, and the hair growth promoting speed is improved.
Description
Technical Field
The invention relates to the technical field of hair growth and development, in particular to a mussel mucin and stem cell composition for hair growth and development and a preparation method thereof.
Background
The hair loss is the phenomenon that hair falls off, the hair which is normally fallen off is the hair in the withdrawal period and the resting period, and the hair which enters the withdrawal period and the hair which newly enters the growing period are continuously in dynamic balance, so that the hair which is normal in quantity can be maintained, the pathological hair loss is the abnormal or excessive hair falling off, the hair loss seriously affects the attractiveness, the hair is treated by using the hair-growing washing and protecting product, but the treatment effect is poor, and mussel mucin and stem cells have certain treatment effects on the hair loss, so that the hair can be used for growing and developing hair.
Chinese patent discloses an anti-hair loss and hair-growing composition and a preparation method thereof, (bulletin No. CN 114344188A), the technology of the patent can effectively supplement scalp nutrition components, regulate scalp cell activity, promote activation of hair follicle stem cells, promote absorption of nutrition components by hair papilla, stimulate hair follicle in a dormant state for a long time to revive and grow so as to further play roles in nourishing hair, fixing hair and promoting hair growth, but the anti-hair loss and hair-growing composition contains more peptides, has larger stimulation to scalp after long-time use, easily generates a large amount of dandruff, has more chemical components, has chemical accumulation to influence body health, has slower drug property absorption of the composition by patients and is unfavorable for hair growth. Accordingly, a mussel mucin and stem cell composition for growing and developing hair and a method for preparing the same are provided by those skilled in the art to solve the problems set forth in the background art.
Disclosure of Invention
The invention aims to provide a mussel mucin and stem cell composition for growing and developing hair and a preparation method thereof, so as to solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 3-9 parts of mussel mucin, 1-3 parts of human hair follicle stem cells, 1-3 parts of human adipose mesenchymal stem cells, 1-3 parts of umbilical cord mesenchymal stem cells, 1-3 parts of stem cell factors, 15-22 parts of hair growth regulator, 10-18 parts of minoxidil, 6-16 parts of sodium hyaluronate and 30-40 parts of deionized water.
As a further scheme of the invention: the hair tonic comprises flos Rhododendri mollis, fructus Psoraleae, rhizoma Zingiberis recens, ginseng radix, radix Angelicae sinensis, cordyceps, saviae Miltiorrhizae radix, carthami flos, folium Platycladi, polygoni Multiflori radix, rhizoma Drynariae, gibberellin, and alcohol at a weight ratio of 15:8:10:15:10:5:20:20:20:30:0.2:1000.
A preparation method of mussel mucin and stem cell composition for growing and developing hair comprises the following steps:
s1, taking out 1-3 parts of human hair follicle stem cells, 1-3 parts of human adipose mesenchymal stem cells, 1-3 parts of umbilical cord mesenchymal stem cells and 1-3 parts of stem cell factors according to parts by weight, and respectively culturing the above materials;
s2, taking 30-40 parts of deionized water according to parts by weight, pouring the deionized water into a reaction kettle, heating the deionized water to a temperature of 40-45 ℃, and preserving the temperature of the deionized water;
s3, adding 3-9 parts of mussel mucin according to parts by weight, stirring, and preserving heat for 5-7 hours after the mussel mucin is melted;
s4, cooling to 20-25 ℃, adding human hair follicle stem cells, human fat mesenchymal stem cells and umbilical cord mesenchymal stem cells according to parts by weight, and stirring;
s5, adding 15-22 parts of a hair tonic, 10-18 parts of minoxidil and 6-16 parts of sodium hyaluronate according to parts by weight, and uniformly stirring;
s6, pouring the mixed solution into a negative pressure tank, removing bubbles through negative pressure treatment, and standing for 1-2 h to obtain a composition;
s7, filling the composition into a glass bottle, and placing the glass bottle into a refrigerating chamber for refrigerating and preserving, wherein the temperature of the refrigerating chamber is 2-12 ℃.
As still further aspects of the invention: the preparation method of the hair tonic comprises the following steps:
s201, crushing rhododendron molle, fructus psoraleae, ginger, ginseng, chinese angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae;
s202, adding crushed rhododendron, fructus psoraleae, ginger, ginseng, angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae into alcohol, soaking for 2-3 days after uniformly mixing;
s203, filtering the materials to obtain a mixed solution;
s204, adding gibberellin into the mixed solution, and uniformly stirring to obtain the hair tonic.
As still further aspects of the invention: the culturing steps of the human hair follicle stem cells in the S1 are as follows:
s011, extracting human hair follicle stem cells from human hindbrain epidermis in a sterile room to obtain hair follicle stem cell suspension;
s012, preparing a serum-free culture medium, adding the serum-free culture medium into the hair follicle stem cell suspension, and performing primary culture to obtain primary hair follicle stem cells;
s013, inoculating the primary hair follicle stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
As still further aspects of the invention: the culturing steps of the umbilical cord mesenchymal stem cells in the step S1 are as follows:
s031, extracting umbilical cord mesenchymal stem cells from fat in a sterile room to obtain umbilical cord mesenchymal stem cell suspension;
s032, preparing a serum-free culture medium, adding the serum-free culture medium into the umbilical cord mesenchymal stem cell suspension, and performing primary culture to obtain primary umbilical cord mesenchymal stem cells;
s033, inoculating the primary umbilical cord mesenchymal stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
As still further aspects of the invention: the stem cell factor in S1 is cultured as follows:
s041, extracting stem cell factors from fat in a sterile room to obtain stem cell factor suspension;
s042, preparing a serum-free culture medium, adding the serum-free culture medium into the stem cell factor suspension, and performing primary culture to obtain the stem cell factor;
s043, inoculating the primary stem cell factor into a culture bottle, and adding a serum-free culture medium for proliferation culture.
As still further aspects of the invention: and the negative pressure in the step S6 is 0-0.6 mpa, and the time is 24-28 min.
Compared with the prior art, the invention has the beneficial effects that:
1. the mussel mucin and stem cell composition for growing and developing hair is used for treating alopecia by pertinence of the mussel mucin and stem cells, and the hair restorer is prepared by adopting traditional Chinese medicines, contains few chemical components, has good effect of growing and developing hair and ensures physical health;
2. the human hair follicle stem cells, the human adipose mesenchymal stem cells, the umbilical cord mesenchymal stem cells and the stem cell factors are all extracted in human body, and the hair growth and development are carried out by regulating the stem cells at the hair loss part, so that the drug absorption completeness is high, the hair growth and development speed is improved, and the hair growth and development effect is facilitated.
Detailed Description
Example 1
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 3 parts of mussel mucin, 1 part of human hair follicle stem cells, 1 part of human adipose mesenchymal stem cells, 1 part of umbilical cord mesenchymal stem cells, 1 part of stem cell factor, 15 parts of germinal agent, 10 parts of minoxidil, 6 parts of sodium hyaluronate and 30 parts of deionized water.
Example two
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 3 parts of mussel mucin, 1 part of human hair follicle stem cells, 1 part of human adipose mesenchymal stem cells, 1 part of umbilical cord mesenchymal stem cells, 1 part of stem cell factor, 22 parts of hair-growing agent, 18 parts of minoxidil, 16 parts of sodium hyaluronate and 40 parts of deionized water.
Example III
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 6 parts of mussel mucin, 2 parts of human hair follicle stem cells, 2 parts of human adipose mesenchymal stem cells, 2 parts of umbilical cord mesenchymal stem cells, 2 parts of stem cell factors, 15 parts of germinal agent, 10 parts of minoxidil, 6 parts of sodium hyaluronate and 30 parts of deionized water.
Example IV
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 6 parts of mussel mucin, 2 parts of human hair follicle stem cells, 2 parts of human adipose mesenchymal stem cells, 2 parts of umbilical cord mesenchymal stem cells, 2 parts of stem cell factors, 22 parts of germinal agent, 18 parts of minoxidil, 16 parts of sodium hyaluronate and 40 parts of deionized water.
Example five
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 9 parts of mussel mucin, 3 parts of human hair follicle stem cells, 3 parts of human adipose mesenchymal stem cells, 3 parts of umbilical cord mesenchymal stem cells, 3 parts of stem cell factors, 15 parts of hair tonic, 10 parts of minoxidil, 6 parts of sodium hyaluronate and 30 parts of deionized water.
Example six
A mussel mucin and stem cell composition for growing and developing hair consists of the following components in parts by weight: 9 parts of mussel mucin, 3 parts of human hair follicle stem cells, 3 parts of human adipose mesenchymal stem cells, 3 parts of umbilical cord mesenchymal stem cells, 3 parts of stem cell factors, 22 parts of hair growth promoting agent, 18 parts of minoxidil, 16 parts of sodium hyaluronate and 40 parts of deionized water.
The contents of the first embodiment, the second embodiment, the third embodiment, the fourth embodiment, the fifth embodiment and the sixth embodiment further include:
the hair tonic comprises flos Rhododendri mollis, fructus Psoraleae, rhizoma Zingiberis recens, ginseng radix, radix Angelicae sinensis, cordyceps, saviae Miltiorrhizae radix, carthami flos, folium Platycladi, polygoni Multiflori radix, rhizoma Drynariae, gibberellin, and alcohol, wherein the weight ratio of the components is 15:8:10:15:10:5:20:20:20:30:0.2:1000, and the preparation method comprises the following steps:
s201, crushing rhododendron molle, fructus psoraleae, ginger, ginseng, chinese angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae;
s202, adding crushed rhododendron, fructus psoraleae, ginger, ginseng, angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae into alcohol, soaking for 2-3 days after uniformly mixing;
s203, filtering the materials to obtain a mixed solution;
s204, adding gibberellin into the mixed solution, and uniformly stirring to obtain the hair tonic.
A preparation method of mussel mucin and stem cell composition for growing and developing hair comprises the following steps:
s1, taking out human hair follicle stem cells, human adipose mesenchymal stem cells, umbilical cord mesenchymal stem cells and stem cell factors according to parts by weight, and respectively culturing the materials, wherein the culturing steps of the human hair follicle stem cells are as follows:
s011, extracting human hair follicle stem cells from human hindbrain epidermis in a sterile room to obtain hair follicle stem cell suspension;
s012, preparing a serum-free culture medium, adding the serum-free culture medium into the hair follicle stem cell suspension, and performing primary culture to obtain primary hair follicle stem cells;
s013, inoculating the primary hair follicle stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
The culture steps of the human adipose-derived mesenchymal stem cells are as follows:
s021, extracting human fat mesenchymal stem cells from human back brain epidermis in a sterile room to obtain human fat mesenchymal stem cell suspension;
s022, preparing a serum-free culture medium, adding the serum-free culture medium into human adipose-derived mesenchymal stem cell suspension, and performing primary culture to obtain primary human adipose-derived mesenchymal stem cells;
s023, inoculating the primary human adipose-derived mesenchymal stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
The umbilical cord mesenchymal stem cells were cultured as follows:
s031, extracting umbilical cord mesenchymal stem cells from fat in a sterile room to obtain umbilical cord mesenchymal stem cell suspension;
s032, preparing a serum-free culture medium, adding the serum-free culture medium into the umbilical cord mesenchymal stem cell suspension, and performing primary culture to obtain primary umbilical cord mesenchymal stem cells;
s033, inoculating the primary umbilical cord mesenchymal stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
The stem cell factor culturing steps are as follows:
s041, extracting stem cell factors from fat in a sterile room to obtain stem cell factor suspension;
s042, preparing a serum-free culture medium, adding the serum-free culture medium into the stem cell factor suspension, and performing primary culture to obtain the stem cell factor;
s043, inoculating the primary stem cell factor into a culture bottle, and adding a serum-free culture medium for proliferation culture;
s2, taking out deionized water according to parts by weight, pouring the deionized water into a reaction kettle, heating the deionized water to 40-45 ℃, and preserving the temperature of the deionized water;
s3, adding mussel mucin according to parts by weight, stirring, and preserving heat for 5-7 h after the mussel mucin is melted;
s4, cooling to 20-25 ℃, adding human hair follicle stem cells, human fat mesenchymal stem cells and umbilical cord mesenchymal stem cells according to parts by weight, and stirring;
s5, adding the hair tonic, minoxidil and sodium hyaluronate according to parts by weight, and uniformly stirring;
s6, pouring the mixed solution into a negative pressure tank, removing bubbles by negative pressure treatment, and standing for 1-2 h to obtain a composition, wherein the negative pressure is 0-0.6 mpa, and the time is 24-28 min;
s7, filling the composition into a glass bottle, and placing the glass bottle into a refrigerating chamber for refrigerating and preserving, wherein the temperature of the refrigerating chamber is 2-12 ℃.
The mussel mucin and stem cell composition for growing and developing hair prepared by the invention is used as an example I, an example II, an example III, an example IV, an example V and an example VI, and an anti-hair-loss hair-growing composition disclosed by a patent net and a preparation method thereof (publication day: 2022-04-15, publication number: CN 114344188A) are selected as a comparative example I;
selecting 70 patients with sparse hair, dividing the patients into seven groups, wherein each 10 patients are one group, respectively using the compositions of the first embodiment, the second embodiment, the third embodiment, the fourth embodiment, the fifth embodiment, the sixth embodiment and the first comparison embodiment for half a year, comparing the seven groups of patients with sparse hair according to the using sequential hair volume of the users after half a year, grading the treatment effect according to the situation, grading the hair concentration before and after the patients with sparse hair, grading the hair concentration by the patient with sparse hair, grading the hair concentration by 1-10 points, taking the total score of each group of 10 people as a grading basis, wherein the treatment effect on the patients with sparse hair is better when the grading is higher,
detecting the chemical content of the composition of the first embodiment, the second embodiment, the third embodiment, the fourth embodiment, the fifth embodiment, the sixth embodiment and the first comparative embodiment by using a chromatograph, scoring the chemical content according to the detection data, scoring 1 to 10 points, taking the total score of 10 people in each group as a scoring basis, wherein the higher the score is, the lower the chemical content is,
the hair growth rate was scored according to the personal feel of the user, the score was 1 to 10 points, the total score of 10 persons in each group was used as the scoring basis, the higher the score, the faster the hair growth rate was, and the results were recorded in table 1 below.
Table 1 scoring table
As can be seen from table 1: the treatment effect scores in the first, second, third, fourth, fifth and sixth embodiments are all higher than those in the first comparative example, and it is known that the treatment effect of the present invention is higher than that in the first comparative example; the chemical content scores in the first, second, third, fourth, fifth and sixth examples are all higher than those in the first comparative example, and the chemical content of the present invention is lower than that in the first comparative example; the hair growth rate scores in the first, second, third, fourth, fifth and sixth examples are all higher than those in the first comparative example, and it is known that the hair growth rate of the present invention is better than that in the first comparative example.
The foregoing description is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical solution of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (9)
1. A mussel mucin and stem cell composition for growing and developing hair, which is characterized by comprising the following components in parts by weight: 3-9 parts of mussel mucin, 1-3 parts of human hair follicle stem cells, 1-3 parts of human adipose mesenchymal stem cells, 1-3 parts of umbilical cord mesenchymal stem cells, 1-3 parts of stem cell factors, 15-22 parts of hair growth regulator, 10-18 parts of minoxidil, 6-16 parts of sodium hyaluronate and 30-40 parts of deionized water.
2. The composition of mussel mucin and stem cells for growing and developing hair according to claim 1, wherein the germinal agent is composed of rhododendron molle, psoralea corylifolia, ginger, ginseng, angelica sinensis, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum, drynaria rhizome, gibberellin and alcohol in a weight ratio of 15:8:10:15:15:10:5:20:20:20:30:0.2:1000.
3. A method for preparing a mussel mucin and stem cell composition for the development of hair according to any one of claims 1-2, characterized by the steps of:
s1, taking out 1-3 parts of human hair follicle stem cells, 1-3 parts of human adipose mesenchymal stem cells, 1-3 parts of umbilical cord mesenchymal stem cells and 1-3 parts of stem cell factors according to parts by weight, and respectively culturing the above materials;
s2, taking 30-40 parts of deionized water according to parts by weight, pouring the deionized water into a reaction kettle, heating the deionized water to a temperature of 40-45 ℃, and preserving the temperature of the deionized water;
s3, adding 3-9 parts of mussel mucin according to parts by weight, stirring, and preserving heat for 5-7 hours after the mussel mucin is melted;
s4, cooling to 20-25 ℃, adding human hair follicle stem cells, human fat mesenchymal stem cells and umbilical cord mesenchymal stem cells according to parts by weight, and stirring;
s5, adding 15-22 parts of a hair tonic, 10-18 parts of minoxidil and 6-16 parts of sodium hyaluronate according to parts by weight, and uniformly stirring;
s6, pouring the mixed solution into a negative pressure tank, removing bubbles through negative pressure treatment, and standing for 1-2 h to obtain a composition;
s7, filling the composition into a glass bottle, and placing the glass bottle into a refrigerating chamber for refrigerating and preserving, wherein the temperature of the refrigerating chamber is 2-12 ℃.
4. The method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 2, wherein the preparation steps of the hair-growing agent are as follows:
s201, crushing rhododendron molle, fructus psoraleae, ginger, ginseng, chinese angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae;
s202, adding crushed rhododendron, fructus psoraleae, ginger, ginseng, angelica, cordyceps sinensis, red sage root, safflower, biota orientalis, polygonum multiflorum and rhizoma drynariae into alcohol, soaking for 2-3 days after uniformly mixing;
s203, filtering the materials to obtain a mixed solution;
s204, adding gibberellin into the mixed solution, and uniformly stirring to obtain the hair tonic.
5. A method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 3, wherein the step of culturing human hair follicle stem cells in S1 comprises the steps of:
s011, extracting human hair follicle stem cells from human hindbrain epidermis in a sterile room to obtain hair follicle stem cell suspension;
s012, preparing a serum-free culture medium, adding the serum-free culture medium into the hair follicle stem cell suspension, and performing primary culture to obtain primary hair follicle stem cells;
s013, inoculating the primary hair follicle stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
6. A method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 3, wherein the step of culturing the human adipose mesenchymal stem cells in S1 comprises the steps of:
s021, extracting human fat mesenchymal stem cells from human back brain epidermis in a sterile room to obtain human fat mesenchymal stem cell suspension;
s022, preparing a serum-free culture medium, adding the serum-free culture medium into human adipose-derived mesenchymal stem cell suspension, and performing primary culture to obtain primary human adipose-derived mesenchymal stem cells;
s023, inoculating the primary human adipose-derived mesenchymal stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
7. A method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 3, wherein the step of culturing umbilical cord mesenchymal stem cells in S1 comprises the steps of:
s031, extracting umbilical cord mesenchymal stem cells from fat in a sterile room to obtain umbilical cord mesenchymal stem cell suspension;
s032, preparing a serum-free culture medium, adding the serum-free culture medium into the umbilical cord mesenchymal stem cell suspension, and performing primary culture to obtain primary umbilical cord mesenchymal stem cells;
s033, inoculating the primary umbilical cord mesenchymal stem cells into a culture flask, and adding a serum-free culture medium for proliferation culture.
8. A method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 3, wherein the step of culturing the stem cell factor in S1 is as follows:
s041, extracting stem cell factors from fat in a sterile room to obtain stem cell factor suspension;
s042, preparing a serum-free culture medium, adding the serum-free culture medium into the stem cell factor suspension, and performing primary culture to obtain the stem cell factor;
s043, inoculating the primary stem cell factor into a culture bottle, and adding a serum-free culture medium for proliferation culture.
9. The method for preparing a mussel mucin and stem cell composition for growing and developing hair according to claim 3, wherein the negative pressure in S6 is 0 to 0.6mpa for 24 to 28min.
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