CN112656872A - Plant alopecia stopping and hair growing liquid as well as preparation method and application thereof - Google Patents

Plant alopecia stopping and hair growing liquid as well as preparation method and application thereof Download PDF

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Publication number
CN112656872A
CN112656872A CN202110108645.7A CN202110108645A CN112656872A CN 112656872 A CN112656872 A CN 112656872A CN 202110108645 A CN202110108645 A CN 202110108645A CN 112656872 A CN112656872 A CN 112656872A
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parts
hair
alopecia
plant
stopping
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戎伟坚
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Youlishu Medical Technology Guangdong Co ltd
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Youlishu Medical Technology Guangdong Co ltd
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Abstract

The invention belongs to the technical field of traditional Chinese medicines, and discloses a plant alopecia stopping and hair growing liquid as well as a preparation method and application thereof. The alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials: calyx seu fructus physalis, cortex Eucommiae, folium Mori, cacumen Platycladi, folium melo, folium Gleditsiae Abnormalis, folium Cannabis, Camellia Chysantha, radix Polygoni Multiflori, herba Ecliptae, stevia rebaudiana (Bertoni) Hemsl, semen Firmianae, radix Adenophorae, Eucalyptus citriodora, Melaleuca leucadendra, herba Spinaciae, and herba Thymi vulgaris. The alopecia stopping and hair growing liquid has a simple formula, can obviously improve the environment of scalp hair follicles, can prevent alopecia, can effectively activate hair follicle cells and induce the growth of new hairs, and has no side effect, safety and health.

Description

Plant alopecia stopping and hair growing liquid as well as preparation method and application thereof
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a plant alopecia stopping and hair growing liquid as well as a preparation method and application thereof.
Background
Alopecia refers to the phenomenon of hair loss. The normal hair loss is the hair in the catagen and telogen phases, and the normal amount of hair can be maintained because the hair entering the catagen phase and the hair newly entering the anagen phase are constantly in dynamic balance. Pathological hair loss refers to abnormal or excessive loss of hair for a number of reasons. Permanent hair loss due to damaged hair follicles can have a certain effect on the appearance instrument. There are many causes of pathological hair loss, and the following factors are mainly considered to cause at present: androgenetic alopecia, nervous alopecia caused by excessive mental stress, alopecia caused by endocrine abnormality, alopecia caused by malnutrition and metabolism abnormality of the body, alopecia caused by infection of various pathogens, and the like. These factors cause deterioration of head ecological environment such as unbalance of scalp nutrition supply, poor blood circulation, excessive fat, hair follicle obstruction, etc., thereby preventing nutrient absorption by hair roots, affecting hair growth, and causing alopecia.
At present, the means for treating alopecia are many, and surgical treatment, chemical drug treatment, Chinese herbal medicine treatment and the like are mainly adopted. The operation treatment is to separate and cultivate healthy hair follicles of the occiput of the patient through an operation method, and then uniformly plant the hair follicles on the alopecia part to grow new hairs. Examples of the chemical drug therapy include oral finasteride (finasteride), topical minoxidil (minoxidil), and the like. However, the operation for treating the alopecia is expensive, the chemical medicine treatment has certain side effect, the chemical synthetic product has certain harm to human bodies after long-term use, and the alopecia is easy to relapse after the medicine is stopped, and the effect is not ideal.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the invention aims to provide the plant alopecia preventing and hair growing liquid, which can improve the environment of scalp hair follicles, prevent hair loss, effectively activate hair follicle cells and induce the growth of new hairs, and has no side effect, safety and health.
The invention also aims to provide a preparation method of the plant alopecia stopping and hair growing liquid.
The invention further aims to provide application of the plant alopecia stopping and hair growing liquid.
The purpose of the invention is realized by the following technical scheme:
the plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials: calyx seu fructus physalis, cortex Eucommiae, folium Mori, cacumen Platycladi, folium melo, folium Gleditsiae Abnormalis, folium Cannabis, Camellia Chysantha, radix Polygoni Multiflori, herba Ecliptae, stevia rebaudiana (Bertoni) Hemsl, semen Firmianae, radix Adenophorae, Eucalyptus citriodora, Melaleuca leucadendra, herba Spinaciae, and herba Thymi vulgaris.
Preferably, the plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 40-70 parts of mullein, 50-100 parts of eucommia bark, 50-100 parts of eucommia leaf, 40-70 parts of frosted mulberry leaf, 50-80 parts of cacumen biotae, 40-70 parts of muskmelon leaf, 40-70 parts of gleditsia sinensis leaf, 30-50 parts of jute leaf, 30-50 parts of golden camellia, 50-100 parts of raw polygonum multiflorum, 50-100 parts of eclipta alba, 30-50 parts of stevia rebaudiana, 30-50 parts of phoenix tree seed, 30-50 parts of adenophora stricta, 30-50 parts of eucalyptus citriodora, 30-50 parts of melaleuca, 30-50 parts of spinach and 30-50 parts of thyme.
More preferably, the plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 50 parts of verbena, 80 parts of eucommia bark, 80 parts of eucommia leaf, 50 parts of frosted mulberry leaf, 60 parts of cacumen biotae, 50 parts of muskmelon leaf, 60 parts of gleditsia sinensis leaf, 40 parts of jute leaf, 40 parts of golden camellia, 80 parts of raw polygonum multiflorum, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seed, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of drynaria, 40 parts of spinach and 40 parts of thyme.
The preparation method of the plant alopecia stopping and hair growing liquid comprises the following operation steps:
(1) cleaning all the traditional Chinese medicine raw materials, and then crushing into ten-mesh coarse powder to obtain traditional Chinese medicine coarse powder;
(2) putting the traditional Chinese medicine powder into a multifunctional extraction tank, adding an ethanol solution which is 10 times the weight of the traditional Chinese medicine coarse powder, wherein the volume percentage concentration of the ethanol solution is 90%, soaking for 48 hours, heating to 65 ℃, starting the multifunctional extraction tank, forcibly circulating for 1 hour, and keeping the temperature for 4 hours; filtering the obtained extracting solution to obtain an alcohol extracting solution;
(3) adding purified water, papain, cellulase and pectinase into the filter residue after the alcohol extraction in the step (2), and adjusting the pH value to 5.5; soaking for 6 hours, heating to 55 ℃, forcibly circulating for 1 hour, and keeping the temperature for 120 min; filtering the obtained extracting solution to obtain enzyme extracting solution; the addition amount of the purified water is 50 times of the weight of the filter residue; the adding amount of the papain is 0.4 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 10 ten thousand u/g; the adding amount of the cellulase is 0.1 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 40 ten thousand u/g; the addition amount of the pectinase is 0.2 percent of the total mass of the filter residue and the purified water, and the enzyme activity is required to be 5 ten thousand u/g;
(4) mixing the alcohol extract obtained in the step (2) and the enzyme extract obtained in the step (3), and concentrating under reduced pressure to obtain an extract with a liquid specific gravity of 1.15;
(5) pumping the extract into an extraction tank, adding medicinal activated carbon, stirring and heating to 45 ℃ with the weight ratio of the extract to the medicinal activated carbon being 5: 1, and filtering after 3 hours; filtering for many times until the filtrate is clear and transparent and has no obvious medicinal taste;
(6) cleaning, sterilizing and packaging to obtain the plant alopecia stopping and hair growing liquid.
The medicinal activated carbon in the step (5) is a mixture of 767 type needle activated carbon, 769 type needle activated carbon, 303 type wood activated carbon and ACTICAR80NE activated carbon.
The plant alopecia stopping and hair growing liquid is applied to preparing alopecia stopping and hair growing products.
The effects of the traditional Chinese medicine raw materials are as follows:
the efficacy and the effect of mullein: has the effects of clearing away heat and toxic materials, stopping bleeding, and removing blood stasis; can be used for treating pneumonia, chronic appendicitis, skin sore, traumatic injury, and traumatic hemorrhage; has effects in inhibiting androgen secretion, and can be used for preventing and treating alopecia and acne due to high androgen level.
The eucommia bark has the following effects: has effects in nourishing liver and kidney, strengthening muscle and bone, and preventing miscarriage; can be used for treating soreness of waist and back, weakness of feet and knees, dribbling urination, threatened abortion, and hypertension.
The efficacy and the effect of the eucommia leaves are as follows: has effects in nourishing liver and kidney, strengthening tendons and bones, and lowering blood pressure; it can be used for treating deficiency of liver and kidney, soreness of waist and knees, and atrophy and weakness of tendons and bones.
The effect of the frost mulberry leaves is as follows: has effects in dispelling pathogenic wind heat, and clearing away lung-heat; it can be used for treating eye diseases, cooling blood, quenching thirst, and arresting sweating; has effects in lowering blood sugar, resisting oxidation, resisting tumor, reducing blood lipid, resisting atherosclerosis, resisting inflammation, preventing alopecia, and promoting hair growth.
Efficacy of cacumen biotae: has effects in clearing away heat, eliminating dampness, purging pathogenic fire, removing toxic materials, cooling blood, stopping bleeding, quenching thirst, promoting hair growth, and blackening hair; can be used for treating hematemesis, hemoptysis, hematochezia, metrorrhagia, metrostaxis, alopecia due to blood heat, and premature gray hair.
Efficacy and effect of the muskmelon leaves: has effects in removing blood stasis and promoting hair growth; it is used for treating infantile malnutrition.
Efficacy of Gleditsia sinensis leaves: has effects in expelling pathogenic wind, removing toxic materials, and promoting hair growth; it is mainly used for treating sore and tinea due to wind-heat, and hair loss.
The efficacy and the effect of the jute leaves are as follows: has effects in regulating qi-flowing, relieving pain, expelling pus, and removing toxic materials; it can be used for treating hemoptysis, hematemesis, metrorrhagia, hematochezia, abdominal pain, dysentery, furuncle, carbuncle, and skin ulcer.
The golden camellia has the following effects: has effects in reducing blood lipid, lowering blood sugar, lowering blood pressure, enhancing immunity, regulating blood flow, preventing atherosclerosis, resisting bacteria, relieving inflammation, clearing away heat and toxic materials, relieving constipation, promoting urination, and removing dampness.
The efficacy and the effect of the raw polygonum multiflorum are as follows: has effects in nourishing liver and kidney, replenishing essence and blood, relieving constipation, removing skin sore toxin, expelling pathogenic wind, and preventing malaria; can be used for treating dizziness, blurred vision, premature gray hair, palpitation, insomnia, spermatorrhea, leukorrhagia, soreness of waist and knees, etc. due to deficiency of liver and kidney and deficiency of essence and blood.
The eclipta prostrata has the following effects: has effects in nourishing liver, invigorating kidney, cooling blood, and stopping bleeding; can be used for treating dizziness, odontoseisis, soreness of waist and back, debility and weakness of lower limbs, and hemorrhage due to blood heat.
The efficacy and the effect of the stevia rebaudiana are as follows: has effects in promoting salivation, quenching thirst, and lowering blood pressure; can be used for treating diabetes and hypertension.
The Chinese parasol has the following effects: has effects in regulating qi, regulating stomach function, invigorating spleen, promoting digestion, and stopping bleeding; can be used for treating gastralgia, diarrhea due to overeating, hernia, premature gray hair, and infantile aphtha.
The function of the root of straight ladybell: has effects in nourishing yin, clearing away heat, moistening lung, eliminating phlegm, invigorating stomach, and promoting salivation; can be used for treating chronic cough due to yin deficiency, cough with excessive phlegm and blood, dry cough with little phlegm, deficiency heat, pharyngitis, and thirst due to body fluid consumption.
The efficacy and the effect of the lemon eucalyptus: has the functions of detumescence and toxin dispersion, is mainly used for treating diarrhea and stomachache, dermatosis and rheumatism and is also one of important raw materials in the perfume industry.
The efficacy and the effect of the cajeput are as follows: the function is mainly used for calming the nerves and tranquilizing.
The efficacy and the effect of spinach: has effects in nourishing blood, stopping bleeding, astringing yin, and moistening dryness; can be used for treating hematochezia, scurvy, thirst, constipation, and stagnation. The spinach contains two trace elements of iron and zinc, can rapidly promote the metabolism of the body, and is matched with the vitamin A and the vitamin B in the spinach to have good hair growth effect.
The thyme has the following effects: has antitussive and anti-inflammatory effects, and can be used for treating cough, acute bronchitis, and laryngitis.
The traditional Chinese medicine considers that the alopecia is generally caused by liver and kidney deficiency, qi and blood deficiency, blood deficiency and wind dryness or liver depression and blood stasis. Hair is mainly related to blood and kidney essence, hair is the remainder of blood, kidney is in the hair, kidney qi deficiency, hair loss and early hair whitening. The nutrition of hair comes from blood, and the growth power comes from kidney essence. Only when the body is full of essence and blood will the hair become black and tough. On the contrary, when liver and kidney are deficient and qi and blood are deficient, essence is not transformed into blood, blood does not nourish hair, hair roots are empty and deficient to fall off and become white, hair filaments are thin, soft and easy to break, and various aging phenomena occur. The causes of seborrheic alopecia are dysfunction of spleen and stomach in the body and cluster of damp-heat, and excessive secretion of grease, namely oily and shiny complexion, large head oil and much scurf are shown outside.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the hair loss-preventing and hair-growing traditional Chinese medicine takes raw tuber fleeceflower root, eclipta alba, eucommia bark and eucommia leaf as monarch drugs, mullein, frosted mulberry leaf, arborvitae tops, muskmelon leaves and gleditsia sinensis leaves as ministerial drugs, and jute leaves, golden camellia, stevia rebaudiana, phoenix tree seed, adenophora stricta, eucalyptus citriodora, cajeput, spinach and thyme as adjuvant drugs, has a reasonable formula, well plays a synergistic effect, achieves the effects of preventing alopecia and growing hair by clearing heat and drying dampness, tonifying liver and kidney, nourishing essence and blood, clearing heat and calming the heart, takes effect quickly, and can effectively treat alopecia caused by various reasons.
(2) The alopecia stopping and hair growing liquid has a simple formula, can obviously improve the environment of scalp hair follicles, can prevent alopecia, can effectively activate hair follicle cells and induce the growth of new hairs, and has no side effect, safety and health. The raw material formula is the conclusion obtained by the inventor through years of practical experience and numerous pharmacological and pharmacodynamic experiments, and the excellent alopecia-stopping and hair-growing effects of the invention cannot be achieved by deletion or replacement of any component in the raw material formula.
(3) The plant alopecia stopping and hair growing liquid has very good treatment effect on alopecia areata, seborrheic alopecia and other types of alopecia, and the existing medicine basically has treatment effect on only one type of alopecia; the alopecia stopping and hair growing liquid can prevent the development of alopecia conditions (namely alopecia stopping effect), has good hair regeneration stimulating effect on alopecia parts, and has no hair regeneration stimulating effect because many existing medicines only have the alopecia stopping effect.
Drawings
Figure 1 is a graph comparing pre-treatment and post-treatment effects.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
The preparation method of the plant alopecia stopping and hair growing liquid in the following examples comprises the following operation steps:
(1) cleaning all the traditional Chinese medicine raw materials, and then crushing into ten-mesh coarse powder to obtain traditional Chinese medicine powder;
(2) putting the traditional Chinese medicine powder into a multifunctional extraction tank, adding an ethanol solution which is 10 times the weight of the traditional Chinese medicine powder, wherein the volume percentage concentration of the ethanol solution is 90%, soaking for 48 hours, heating to 65 ℃, starting the multifunctional extraction tank, forcibly circulating for 1 hour, and keeping the temperature for 4 hours; filtering the obtained extractive solution to obtain alcoholic extractive solution
(3) Adding purified water, papain, cellulase and pectinase into the filter residue after the alcohol extraction in the step (2), and adjusting the pH value to 5.5; soaking for 6 hours, heating to 55 ℃, forcibly circulating for 1 hour, and keeping the temperature for 120 min; filtering the obtained extracting solution to obtain enzyme extracting solution; the addition amount of the purified water is 50 times of the weight of the filter residue; the adding amount of the papain is 0.4 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 10 ten thousand u/g; the adding amount of the cellulase is 0.1 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 40 ten thousand u/g; the addition amount of the pectinase is 0.2 percent of the total mass of the filter residue and the purified water, and the enzyme activity is required to be 5 ten thousand u/g;
(4) mixing the alcohol extract obtained in the step (2) and the enzyme extract obtained in the step (3), and concentrating under reduced pressure to obtain an extract with a liquid specific gravity of 1.15;
(5) pumping the extract into an extraction tank, adding medicinal activated carbon (mixture of 767 type, 769 type, 303 type wood activated carbon and ACTICAR80NE activated carbon) at a weight ratio of 5: 1, stirring, heating to 45 deg.C for 3 hr, and filtering; filtering for many times until the filtrate is clear and transparent and has no obvious medicinal taste;
(6) cleaning, sterilizing and packaging to obtain the plant alopecia stopping and hair growing liquid.
Comparative example 1
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 50 parts of verbena, 80 parts of eucommia bark, 80 parts of eucommia leaf, 50 parts of frosted mulberry leaf, 60 parts of cacumen biotae, 50 parts of muskmelon leaf, 60 parts of gleditsia sinensis leaf, 40 parts of jute leaf, 40 parts of golden camellia, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seed, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of cajeput, 40 parts of spinach and 40 parts of thyme.
Comparative example 2
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 80 parts of eucommia bark, 80 parts of eucommia leaves, 50 parts of frosted mulberry leaves, 60 parts of cacumen biotae, 50 parts of muskmelon leaves, 60 parts of gleditsia sinensis leaves, 40 parts of jute leaves, 40 parts of golden camellia, 80 parts of raw polygonum multiflorum, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seeds, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of cajeput, 40 parts of spinach and 40 parts of thyme.
Comparative example 3
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 50 parts of verbena, 80 parts of eucommia bark, 80 parts of eucommia leaf, 50 parts of frosted mulberry leaf, 60 parts of cacumen biotae, 50 parts of muskmelon leaf, 60 parts of gleditsia sinensis leaf, 40 parts of jute leaf, 40 parts of golden camellia, 80 parts of raw polygonum multiflorum, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seed, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of cajeput and 40 parts of spinach.
Example 1
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 50 parts of verbena, 80 parts of eucommia bark, 80 parts of eucommia leaf, 50 parts of frosted mulberry leaf, 60 parts of cacumen biotae, 50 parts of muskmelon leaf, 60 parts of gleditsia sinensis leaf, 40 parts of jute leaf, 40 parts of golden camellia, 80 parts of raw polygonum multiflorum, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seed, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of drynaria, 40 parts of spinach and 40 parts of thyme.
(1) The hair on the area of 2cm multiplied by 2cm on the back of the rabbit is removed by 10 percent sodium sulfide, then the plant hair loss stopping and hair growth promoting liquid prepared in the embodiment is smeared on the hair removal area twice a day, the average length of the new hair is measured to be 0.75, 1.55, 2.78, 5.65, 8.99 and 13.32 mm on days 5, 10, 15, 20, 25 and 30, and the average length of the new hair is measured to be 0.45, 0.88, 1.56, 2.99, 3.56 and 5.35 mm on blank control. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Meanwhile, the plant alopecia stopping and hair growing liquid prepared in the comparative example 1 is used for carrying out the comparative experiment, and the average length of the grown new hairs is respectively measured to be 0.60 mm, 1.02 mm, 2.05 mm, 5.10 mm, 7.20 mm and 11.20 mm in days 5, 10, 15, 20, 25 and 30; the comparative experiment is carried out by using the plant alopecia stopping and hair growing liquid prepared in the comparative example 2, and the average lengths of the grown new hairs on days 5, 10, 15, 20, 25 and 30 are respectively measured to be 0.65, 1.35, 2.03, 5.80, 8.77 and 12.90 mm; the comparative experiment is carried out by using the plant alopecia stopping and hair growing liquid prepared in the comparative example 3, and the average lengths of the grown new hairs on days 5, 10, 15, 20, 25 and 30 are respectively measured to be 0.59, 1.09, 2.25, 5.45, 8.06 and 12.60 millimeters; data analysis shows that the plant alopecia stopping and hair growing liquid has better effect on promoting the growth and the attack than the plant alopecia stopping and hair growing liquid obtained by the comparison ratio 1-3.
(2) Injecting the testosterone propionate in 2.5ml/kg dose into rabbit, molding for 30 days, removing hair on the back of the rabbit by 2cm × 2cm area with 10% sodium sulfide on the 31 st day, then coating the hair removal area with the plant hair-loss stopping and hair-growing liquid prepared in the embodiment twice a day, wherein the average length of the new hair is measured to be 0.72, 1.07, 1.66, 2.32, 4.78 and 8.98 mm on the 5 th, 10 th, 15 th, 20 th, 25 th and 30 th days, and the average length of the new hair is measured to be 0.43, 0.49, 0.56, 1.95, 2.77 and 3.35 mm on the blank control. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Meanwhile, the plant alopecia stopping and hair growing liquid prepared in the comparative example 1 is used for carrying out the comparative experiment, and the average length of the grown new hairs is respectively measured to be 0.52, 0.89, 1.06, 2.08, 4.28 and 8.00 mm in days 5, 10, 15, 20, 25 and 30; the comparative experiment is carried out by using the plant alopecia stopping and hair growing liquid prepared in the comparative example 2, and the average lengths of the grown new hairs on days 5, 10, 15, 20, 25 and 30 are respectively measured to be 0.60 mm, 0.92 mm, 1.25 mm, 1.96 mm, 4.78 mm and 6.99 mm; the comparative experiment is carried out by using the plant alopecia stopping and hair growing liquid prepared in the comparative example 3, and the average lengths of the grown new hairs on days 5, 10, 15, 20, 25 and 30 are respectively measured to be 0.59, 0.87, 1.05, 1.96, 3.25 and 7.25 mm; data analysis shows that the plant alopecia stopping and hair growing liquid has better effect on promoting the growth and the attack than the plant alopecia stopping and hair growing liquid obtained by the comparison ratio 1-3.
Example 2
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 40 parts of mullein, 50 parts of eucommia bark, 50 parts of eucommia leaves, 40 parts of frosted mulberry leaves, 50 parts of cacumen biotae, 40 parts of muskmelon leaves, 40 parts of gleditsia sinensis leaves, 30 parts of jute leaves, 30 parts of golden camellia, 50 parts of raw polygonum multiflorum, 50 parts of eclipta alba, 30 parts of stevia rebaudiana, 30 parts of phoenix tree seeds, 30 parts of adenophora stricta, 30 parts of eucalyptus citriodora, 30 parts of cajeput, 30 parts of spinach and 30 parts of thyme.
The hair on the back of the rabbit is removed by 2cm multiplied by 2cm by 10 percent sodium sulfide, then the plant hair loss stopping and hair growth promoting liquid prepared in the embodiment is smeared on the hair loss area twice a day, the average length of the new hair is measured to be 0.77, 1.43, 2.73, 5.59, 8.76 and 13.56 mm on days 5, 10, 15, 20, 25 and 30, and the average length of the new hair is measured to be 0.48, 0.93, 1.58, 2.78, 3.53 and 5.22 on blank control. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Injecting the rabbit according to the dosage of testosterone propionate 2.5ml/kg, molding for 30 days, removing hair on the back of the rabbit by 2cm × 2cm area with 10% sodium sulfide on the 31 st day, then coating the hair removal area with the plant hair removal and hair growth liquid prepared in the embodiment twice a day, wherein the average length of the new hair is measured to be 0.72, 1.35, 1.58, 2.45, 4.32 and 9.05 mm on the 5 th, 10 th, 15 th, 20 th, 25 th and 30 th days, and the average length of the new hair is measured to be 0.42, 0.52, 0.61, 1.88, 2.80 and 3.40 on the blank control. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Example 3
The plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 70 parts of verbena, 100 parts of eucommia bark, 100 parts of eucommia leaves, 70 parts of frost mulberry leaves, 80 parts of cacumen biotae, 70 parts of muskmelon leaves, 70 parts of gleditsia sinensis leaves, 50 parts of jute leaves, 50 parts of golden camellia, 100 parts of raw polygonum multiflorum, 100 parts of eclipta alba, 50 parts of stevia rebaudiana, 50 parts of phoenix tree seeds, 50 parts of adenophora stricta, 50 parts of eucalyptus citriodora, 50 parts of cajeput, 50 parts of spinach and 50 parts of thyme.
The hair on the back of the rabbit is removed by 2cm multiplied by 2cm by 10 percent sodium sulfide, then the plant hair loss stopping and hair growth promoting liquid prepared in the embodiment is smeared on the hair loss area twice a day, the average length of the new hair is measured to be 0.80, 1.49, 2.55, 6.05, 8.85 and 13.89 mm on days 5, 10, 15, 20, 25 and 30, and the average length of the new hair is measured to be 0.48, 0.90, 1.67, 2.87, 3.56 and 5.77 on blank control. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Injecting the testosterone propionate in a dosage of 2.5ml/kg into rabbits, molding for 30 days, removing hair on the back of the rabbits by 2cm × 2cm with 10% sodium sulfide on the 31 st day, then coating the hair removal area with the plant hair-loss stopping and hair-growing liquid prepared in the embodiment twice a day, wherein the average length of the new hair grown in the 5 th, 10 th, 15 th, 20 th, 25 th and 30 th days is 0.85 mm, 1.35 th, 1.62 th, 2.40 th, 4.98 th and 8.53 th mm, and the average length of the new hair grown in the blank control is 0.45 th, 0.50 th, 0.57 th, 1.89 th, 2.62 th and 3.45 th days. Data analysis shows that from day 10, the plant alopecia stopping and hair growing liquid has significant difference in new hair length compared with the blank control group.
Clinical effects example 1:
240 patients with alopecia areata and seborrheic alopecia, between the ages of 30 and 70 years, 160 men and 80 women are selected. Patients were randomized into 6 groups of 40 people each, of which 3 were control groups and the remaining 3 were experimental groups. The plant alopecia preventing and hair growing liquid of the comparative examples 1 to 3 is respectively applied to the control group, and the plant alopecia preventing and hair growing liquid of the examples 1 to 3 is respectively applied to the other 3 groups. The application method comprises cleaning head with clear water, dipping with sponge, wiping with sponge, twice daily for 4 weeks without using other products, observing hair of patient with naked eye before and 28 days after use, and counting hair change condition after use. The classification is performed according to the following scenario description:
clinical cure: the symptoms of alopecia are completely eliminated, and new hair is generated;
the effect is shown: eliminating alopecia symptoms;
the method has the following advantages: the alopecia symptoms are basically eliminated, and the alopecia is slowed down;
and (4) invalidation: no change or aggravation in all aspects compared to before treatment.
The results 28 days after using the plant alopecia stopping and hair growth promoting liquid are shown in table 1:
TABLE 1 results of hair loss prevention and hair growth of examples and comparative examples
Group of Number of examples Clinical recovery Show effect Improvement of life Invalidation
Example 1 40 28 8 4 0
Example 2 40 30 8 2 0
Example 3 40 29 7 4 0
Comparative example 1 40 20 6 14 0
Comparative example 2 40 22 7 11 0
Comparative example 3 40 23 5 12 0
The above results show that examples have a P < 0.05 in clinical recovery compared to comparative examples, and examples 1-3 have significant differences in clinical recovery and significant results compared to comparative examples 1-3.
As can be seen from table 1, the plant alopecia preventing and hair growing solutions obtained in examples 1 to 3 indeed have the effects of significantly improving the environment of scalp hair follicles, preventing hair loss, effectively activating hair follicle cells, and inducing the growth of new hairs. The formula of the raw materials is reasonable, the components play a synergistic effect, the comparative examples 1-3 of individual raw material components are relatively eliminated, and the plant alopecia-stopping hair-growing liquid provided by the invention can greatly improve the alopecia phenomenon of patients and promote hair growth.
Clinical effects example 2:
the patient declines to a certain age, male, is 39 years old, has 4 parts of alopecia areata on the head, has been half a year, has been regenerated by using octopus 101 for 3 months and is ineffective by using Hanyong for 9+ 94 months, the alopecia areata alopecia-preventing and hair-growing liquid prepared by the embodiment 1 of the invention is applied to the affected part for 2 times a day, fine hair grows on the alopecia areata part after 1 treatment course (28 days is a treatment course), thick black hair grows after 1 treatment course is continuously used, and thick and short black hair grows after 1 treatment course is continuously used.
Clinical effects example 3:
patients are in a portrait state and female, the age is 50 years, the hair loss is 6 months, the patients are in restlessness, irritability and much hair loss for half a year, more than one hundred roots of the patients can be detected every day, the hair is thin and yellow, and the hair can fall off by touching, and the hair loss is reduced after the plant alopecia stopping and hair growing liquid obtained in the embodiment 2 of the invention is used for half a treatment course (28 days is a treatment course); after the hair-care product is continuously used for half a treatment course, the hair loss of a patient is obviously reduced (40-50 hair loss per day); after a treatment course, the hair of the patient gradually flourishes and becomes bright.
Clinical effects example 4:
a patient is a male, 46 years old, seborrheic alopecia and scalp top hair are sparse, the regeneration of hair by using octopus 101 is not effective for 4 months, and the regeneration of hair by using Hanyong 9+ 93 months is also not effective, the alopecia stopping and hair growing liquid prepared by the method provided by the invention in the embodiment 1 is coated on the scalp top for 2 times a day, fine hair begins to grow on the scalp top after 1 treatment course (28 days is a treatment course), coarse black hair is formed after 1 treatment course, and the coarse black hair grows after 1 treatment course.
Clinical effects example 5:
when a patient is a male patient, the patient is 26 years old, the alopecia type is alopecia totalis, the alopecia is manifested as full alopecia at the head top and the temporal part of the hair is completely removed, and the disease is about two years, the plant alopecia stopping and hair growing liquid obtained in the embodiment 1 of the invention is coated on the head top for 2 times a day, the picture is taken before the treatment as shown in (a) in figure 1, and after 1 treatment course (28 days is one treatment course), the scalp starts to grow vellus hairs as shown in (b) in figure 1; the hair starts growing out in the partially balded area after 2 treatment courses are used, as shown in (c) of fig. 1; after 3 treatment courses, black hair was regrown over the bald area as shown in fig. 1 (d).
Application example 1:
the plant alopecia preventing and hair growing liquid obtained in the example 1 is prepared into alopecia preventing and hair growing shampoo, and the shampoo comprises the following components in percentage by weight:
the preparation method of the anti-hair loss and hair growth shampoo comprises the following steps:
(1) dispersing guar gum hydroxypropyl trimethyl ammonium chloride in deionized water, and uniformly stirring; and adding sodium laureth sulfate, lauramidopropyl betaine, cocamide DEA, polyquaternium-22 and allantoin, and mixing and dispersing uniformly to obtain phase A.
(2) Uniformly mixing EDTA-2 sodium, DMDM hydantoin, methylisothiazolinone, methylchloroisothiazolinone, magnesium chloride and magnesium nitrate to obtain a phase B;
(3) stirring and dispersing the phase A uniformly, adding the plant alopecia stopping and hair growing liquid under the stirring state, stirring for 10 minutes, adding the phase B, and uniformly mixing; adding edible essence (green tea), sodium chloride and citric acid one by one to obtain shampoo for preventing alopecia and promoting hair growth.
According to the practical effect of using the anti-hair loss and hair growth shampoo by a plurality of volunteers, the anti-hair loss and hair growth shampoo has a very good treatment effect on alopecia areata, seborrheic alopecia and other types, can prevent the development of alopecia conditions (namely the anti-hair loss effect), and has a good hair regeneration stimulation effect on parts which have already been subjected to hair loss.
Application example 2:
the plant alopecia stopping and hair growing liquid obtained in the example 1 is prepared into a scalp hair follicle nutrient solution (a leave-on type), and the scalp hair follicle nutrient solution consists of the following components in percentage by weight:
the preparation method of the scalp hair follicle nutrient solution (leave-on type) comprises the following steps:
(1) mixing hyaluronic acid, 1, 3-butanediol, PEG/PPG-17/6 copolymer, Kappaphycus ALVAREZII (KAPPAPPHYCUS ALVAREZII) extract, and Caesalpinia spinosa (CAESALPINIA SPINOSA) fruit extract in deionized water, and dispersing uniformly to obtain phase A;
(2) mixing concentrated flower water or essential oil and PEG-40/45 hydrogenated castor oil to obtain phase B;
(3) mixing EDTA-2 sodium and DMDM hydantoin to obtain phase C;
(4) stirring and dispersing the phase A uniformly, adding the plant alopecia stopping and hair growing liquid under the stirring state, stirring for 10 minutes, adding the phase B, and uniformly mixing; adding phase C to obtain scalp hair follicle nutrient solution (leave-on type).
According to the practical effect of using the scalp hair follicle nutrient solution (leave-on type) by a plurality of volunteers, the scalp hair follicle nutrient solution (leave-on type) has very good treatment effect on alopecia areata, seborrheic alopecia and other types, can prevent the development of alopecia conditions (namely, alopecia stopping effect) and has good hair regeneration stimulation effect on the parts which have already been subjected to alopecia.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (6)

1. A plant alopecia stopping and hair growing liquid is characterized in that: the alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials: calyx seu fructus physalis, cortex Eucommiae, folium Mori, cacumen Platycladi, folium melo, folium Gleditsiae Abnormalis, folium Cannabis, Camellia Chysantha, radix Polygoni Multiflori, herba Ecliptae, stevia rebaudiana (Bertoni) Hemsl, semen Firmianae, radix Adenophorae, Eucalyptus citriodora, Melaleuca leucadendra, herba Boraginae and herba Thymi vulgaris.
2. The plant alopecia stopping and hair growing liquid as claimed in claim 1, wherein: the plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 40-70 parts of mullein, 50-100 parts of eucommia bark, 50-100 parts of eucommia leaf, 40-70 parts of frosted mulberry leaf, 50-80 parts of cacumen biotae, 40-70 parts of muskmelon leaf, 40-70 parts of gleditsia sinensis leaf, 30-50 parts of jute leaf, 30-50 parts of golden camellia, 50-100 parts of raw polygonum multiflorum, 50-100 parts of eclipta alba, 30-50 parts of stevia rebaudiana, 30-50 parts of phoenix tree seed, 30-50 parts of adenophora stricta, 30-50 parts of eucalyptus citriodora, 30-50 parts of melaleuca, 30-50 parts of spinach and 30-50 parts of thyme.
3. The plant alopecia stopping and hair growing liquid as claimed in claim 1, wherein: the plant alopecia stopping and hair growing liquid is prepared from the following traditional Chinese medicine raw materials in parts by weight: 50 parts of verbena, 80 parts of eucommia bark, 80 parts of eucommia leaf, 50 parts of frosted mulberry leaf, 60 parts of cacumen biotae, 50 parts of muskmelon leaf, 60 parts of gleditsia sinensis leaf, 40 parts of jute leaf, 40 parts of golden camellia, 80 parts of raw polygonum multiflorum, 80 parts of eclipta alba, 40 parts of stevia rebaudiana, 40 parts of phoenix tree seed, 40 parts of adenophora stricta, 40 parts of eucalyptus citriodora, 40 parts of cajeput, 40 parts of spinach and 40 parts of thyme.
4. The preparation method of the plant alopecia stopping and hair growing liquid as claimed in claim 1, characterized by comprising the following operation steps:
(1) cleaning all the traditional Chinese medicine raw materials, and then crushing into ten-mesh coarse powder to obtain traditional Chinese medicine coarse powder;
(2) putting the traditional Chinese medicine powder into a multifunctional extraction tank, adding an ethanol solution which is 10 times the weight of the traditional Chinese medicine coarse powder, wherein the volume percentage concentration of the ethanol solution is 90%, soaking for 48 hours, heating to 65 ℃, starting the multifunctional extraction tank, forcibly circulating for 1 hour, and keeping the temperature for 4 hours; filtering the obtained extracting solution to obtain an alcohol extracting solution;
(3) adding purified water, papain, cellulase and pectinase into the filter residue after the alcohol extraction in the step (2), and adjusting the pH value to 5.5; soaking for 6 hours, heating to 55 ℃, forcibly circulating for 1 hour, and keeping the temperature for 120 min; filtering the obtained extracting solution to obtain enzyme extracting solution; the addition amount of the purified water is 50 times of the weight of the filter residue; the adding amount of the papain is 0.4 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 10 ten thousand u/g; the adding amount of the cellulase is 0.1 percent of the total mass of the filter residue and the purified water, and the enzyme activity requires 40 ten thousand u/g; the addition amount of the pectinase is 0.2 percent of the total mass of the filter residue and the purified water, and the enzyme activity is required to be 5 ten thousand u/g;
(4) mixing the alcohol extract obtained in the step (2) and the enzyme extract obtained in the step (3), and concentrating under reduced pressure to obtain an extract with a liquid specific gravity of 1.15;
(5) pumping the extract into an extraction tank, adding medicinal activated carbon, stirring and heating to 45 ℃ with the weight ratio of the extract to the medicinal activated carbon being 5: 1, and filtering after 3 hours; filtering for many times until the filtrate is clear and transparent and has no obvious medicinal taste;
(6) cleaning, sterilizing and packaging to obtain the plant alopecia stopping and hair growing liquid.
5. The method of claim 4, wherein: the medicinal activated carbon in the step (5) is a mixture of 767 type needle activated carbon, 769 type needle activated carbon, 303 type wood activated carbon and ACTICAR80NE activated carbon.
6. The use of the plant alopecia preventing and hair growing liquid according to claim 1 in the preparation of alopecia preventing and hair growing products.
CN202110108645.7A 2021-01-26 2021-01-26 Plant alopecia stopping and hair growing liquid as well as preparation method and application thereof Pending CN112656872A (en)

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CN113384642A (en) * 2021-07-15 2021-09-14 忧立舒医药科技(广东)有限公司 Natural extraction compound with acne removing effect and preparation method and application thereof
CN113398036A (en) * 2021-07-15 2021-09-17 忧立舒医药科技(广东)有限公司 No-wash plant scalp hair cleaning agent and preparation method and application thereof
CN113509426A (en) * 2021-07-19 2021-10-19 忧立舒医药科技(广东)有限公司 Plant extract compound capable of repairing striae gravidarum and striae adiposity and preparation method and application thereof

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CN104435358A (en) * 2014-12-03 2015-03-25 谢秉权 Plant anti-hair-loss hair nourishing and hair growing liquid and preparation method thereof
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CN103784590A (en) * 2014-02-19 2014-05-14 钱坤 Alopecia-preventing hair-growing traditional Chinese medicine formula
CN104435358A (en) * 2014-12-03 2015-03-25 谢秉权 Plant anti-hair-loss hair nourishing and hair growing liquid and preparation method thereof
CN105749242A (en) * 2016-05-09 2016-07-13 王月 Hair growth lotion and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113384642A (en) * 2021-07-15 2021-09-14 忧立舒医药科技(广东)有限公司 Natural extraction compound with acne removing effect and preparation method and application thereof
CN113398036A (en) * 2021-07-15 2021-09-17 忧立舒医药科技(广东)有限公司 No-wash plant scalp hair cleaning agent and preparation method and application thereof
CN113509426A (en) * 2021-07-19 2021-10-19 忧立舒医药科技(广东)有限公司 Plant extract compound capable of repairing striae gravidarum and striae adiposity and preparation method and application thereof

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Application publication date: 20210416