CN117643563A - Antibacterial mite-killing cream and preparation method thereof - Google Patents
Antibacterial mite-killing cream and preparation method thereof Download PDFInfo
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- CN117643563A CN117643563A CN202410118216.1A CN202410118216A CN117643563A CN 117643563 A CN117643563 A CN 117643563A CN 202410118216 A CN202410118216 A CN 202410118216A CN 117643563 A CN117643563 A CN 117643563A
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Abstract
A bacteriostatic mite-killing cream and a preparation method thereof belong to the technical field of cosmetics, and the components of the bacteriostatic mite-killing cream comprise frog skin antibacterial peptide, cecropin, anti-inflammatory peptide, bombesin, conotoxin peptide, tea polyphenol, kaempferin, plant extract, thickener, humectant and the like. The plant extract comprises herba Salvia officinalis extract, fructus Arctii extract and fructus Aurantii Immaturus extract. The antibacterial peptide, the anti-inflammatory peptide, the antibacterial and mite-killing component and the plant extract are used together, so that the antibacterial and mite-killing effect is effectively improved; the composite humectant and the surface film forming technology are adopted, so that the long-acting moisturizing, slow release and absorption can be realized, the long-acting antibacterial and mite-expelling effects can be realized, meanwhile, the skin allergy resistance can be regulated, the skin immunity can be improved, the invasion of mites and bacteria can be relieved, and the composite humectant has good practical value.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an antibacterial mite-killing cream and a preparation method thereof.
Background
Bacteria and mites can be parasitic on human skin, attack the skin, and cause skin to produce symptoms such as itching, dermatitis, acne, rash, scabies, etc. Bacteria and mites grow on the skin, on the one hand because of hygiene problems; on the other hand, along with the continuous improvement of the technological development and the improvement of the living standard of people, various cosmetics are applied to the skin of the face and the body, and in order to improve the beautifying and skin care effects, various nutrients are added into the cosmetics to promote the breeding of bacteria and mites.
The conventional antibacterial and mite-killing method generally adopts washing articles such as liquid laundry detergent, bath lotion, soap, facial cleanser, hand lotion and shampoo, and the like, and antibacterial and mite-killing components are added to keep personal hygiene, for example, a mild type mite-killing and itching-relieving bath lotion disclosed in CN111110577B and a preparation method thereof, an antibacterial and mite-killing cosmetic soap disclosed in CN114634849A and a preparation method thereof, and a composition containing rice hull extracts and used for removing dandruff, controlling oil and inhibiting and killing mites disclosed in CN 115569105B. The application of a plant extract disclosed in CN113304085B in preparing cosmetics with antibacterial and mite-killing functions also can be realized by adding antibacterial and mite-killing components into cosmetics. However, the principle of bacteriostasis and mite removal of the toilet articles is mainly that bacteria and mites are washed away, the detergent is washed away along with the bacteria and mites, and bacteria and mites can be repeatedly bred when the toilet articles are frequently contacted with bacteria and mite sources, so that the skin prevention promoting effect is extremely small. The antibacterial and mite-removing cosmetic is smeared on the skin and stays on the surface layer of the skin for a long time, so that good antibacterial and mite-removing effects and repeated breeding prevention effects can be achieved. However, the existing cosmetics are added with antibacterial and mite-killing components, generally chemical agents, plant extracts, a mixture of the chemical agents and the plant extracts, and the components are simply mixed, so that the prepared cosmetics are smeared on the face, and because the skin absorbs water quickly and moisture retention components evaporate quickly, the antibacterial and mite-killing protection time is short, the efficient antibacterial and mite-killing time is generally 3-4 hours, and in addition, the antibacterial and mite-killing components belong to bactericides, and if the antibacterial and mite-killing components are not well mixed, side effects of allergy and damage to the cortex can be caused.
Disclosure of Invention
The antibacterial and mite-killing effect time is short and the prevention effect is poor aiming at the existing washing products; and the existing antibacterial mite-removing cosmetics are short in aging time, and are easy to cause skin allergy, damage the skin and the like. The invention provides an antibacterial and mite-killing cream and a preparation method thereof, which adopt antibacterial peptide, anti-inflammatory peptide, antibacterial and mite-killing components and plant extracts for cooperation, thereby effectively improving antibacterial and mite-killing effects; by adopting the composite humectant and surface film forming technology, the long-acting moisture retention, slow release and absorption can be realized, the long-acting antibacterial and mite-expelling effects can be realized, meanwhile, the skin allergy resistance can be regulated, the skin immunity can be improved, and the invasion of mites and bacteria can be lightened. The specific technical scheme is as follows:
the antibacterial and mite-killing cream comprises, by mass, 1-3 parts of frog skin antibacterial peptide Dermaseptin, 0.01-0.05 part of Cecropin B, 2-6 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide, 0.5-1 part of frog skin peptide Bombesin, 0.3-0.5 part of conotoxin peptide, 0.5-2 parts of tea polyphenol, 0.1-1 part of kaki pepper extract, 5-10 parts of plant extract, 0.1-0.5 part of hydroxyethyl carboxymethyl cellulose, 0.1-0.5 part of sodium hyaluronate, 0.1-0.5 part of butanediol, 0.1-0.5 part of triglyceride, 3-6 parts of GTCC, 0.1-1 part of ceramide and 5-20 parts of purified water.
According to the technical scheme, the plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is (3-5) = (0.5-1): (1-2).
In the above technical scheme, the sage extract is an acetone extract of sage.
In the above technical scheme, the burdock fruit extract is an aqueous extract of burdock fruit.
In the above technical scheme, the fructus Aurantii Immaturus extract is methanol or ethanol extract of fructus Aurantii Immaturus.
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 50% of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 38-40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 38-40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature, and then freeze-drying to obtain modified composite peptide C;
S2, respectively preheating the modified composite peptide C and the GTCC to 38-40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 1-3 min at 38-40 ℃, and preserving heat to obtain a premix D;
s3: according to the mass portion ratio, the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the rest 50% of hydroxyethyl carboxymethyl cellulose are uniformly mixed at the temperature of 80-85 ℃, then the temperature is reduced to 45-50 ℃, conotoxin peptide, tea polyphenol, kaempferide and plant extract are sequentially added, the mixture is continuously uniformly mixed, then the temperature is reduced to 38-40 ℃, the premix D is added, and the mixture is slowly mixed for 1-3 min, so that the antibacterial mite-removing cream is obtained.
In the above method S1, the low temperature drying temperature is not more than 45 ℃.
In S1 of the above method, the lyophilization is performed using liquid nitrogen.
In the S2 of the method, the rotating speed of the slow mixing is 20 r/min-40 r/min.
In the S3 of the method, the rotating speed of the slow mixing is 20 r/min-40 r/min.
Compared with the prior art, the antibacterial mite-killing cream and the preparation method have the beneficial effects that:
1. the antibacterial mite-killing cream is added with the frog skin antibacterial peptide Dermaseptin and the Cecropin B, has good antibacterial effect on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, and can also improve the skin immunity. Anti-inflammatory peptide Anti-Inflammatory Peptide 1 is added, has Anti-inflammatory and antiallergic effects, and can be used for eliminating acne. The Bombesin is added, so that the Bombesin has oxidation resistance, can promote and maintain the stability of skin grease, can improve the antibacterial effect when the Bombesin is matched with antibacterial peptide and anti-inflammatory peptide, can not cause excessive skin dryness and can not cause excessive skin grease secretion to cause bacterial breeding, and can not cause acne, rash and scabies.
2. The antibacterial mite-killing cream is added with conotoxin peptide, tea polyphenol and kaempferide for cooperation, and has good mite-killing and mite-inhibiting effects. Tea polyphenol is a natural antioxidant, has strong antibacterial and anti-inflammatory effects, can effectively kill mites and germs, and can also protect skin health. The kavain is a natural bactericide, can effectively kill germs and mites, and can promote blood circulation and improve skin quality. The conotoxin peptide is a bionic peptide artificially synthesized by biologically simulating the toxin secreted by the conotoxin, has the effects of assisting in removing mites and inhibiting mites, can reduce the component content of the sanguisorbin, reduces the stimulation to the skin, can specifically block the current conduction, inhibit the muscle contraction and achieve the effect of reducing wrinkles.
3. The antibacterial and mite-removing cream is added with plant extracts, specifically a sage extract, a burdock extract and an immature bitter orange extract, and has the effects of diminishing inflammation, inhibiting bacteria and removing mites. Wherein, the burdock extract component has good anti-inflammatory and antibacterial effects on oily skin.
4. The antibacterial and mite-killing cream uses a composite humectant, wherein butanediol has hygroscopicity, can absorb ambient humidity, and keeps long-term moisture retention of the cream after being smeared; the GTCC has good water locking performance, slows down the water emission in the skin and the cream, and keeps the antibacterial and mite-removing long-acting performance of the active substances; the ceramide and the triglyceride can regulate the water absorption performance of cells and the lipid barrier structure among cells, so as to realize the regulation of the absorption order of cream components and prevent the absorption disorder; the sodium hyaluronate is a polysaccharide high molecular humectant, the aqueous solution expands, and combines a large amount of moisture, so that the sodium hyaluronate has a soft film-forming effect, can not absorb moisture in skin, and is also used as a thickener.
5. The antibacterial mite-killing cream is added with the hydroxyethyl carboxymethyl cellulose which is used as a coating agent and a thickening agent, and the hydroxyethyl carboxymethyl cellulose coats Cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide and Bombesin, so that the release time of the peptide can be prolonged, the long-acting antibacterial effect can be achieved through the medium-and-later-period release, the peptide absorption speed can be delayed, the time-division absorption can be realized, and the skin cell absorption disorder can not be caused. Hydroxyethyl carboxymethyl cellulose as a coating agent has a certain water locking effect on the surface of the peptide, and can prevent the peptide from reducing activity after long-time skin drying. The long-acting composite humectant and the hydroxyethyl carboxymethyl cellulose are matched for use, so that the long-acting composite humectant can keep the absorption activity of peptide for a longer time, improve the absorption capacity, improve the self-repairing and anti-sensitization of skin and have long-acting antibacterial effect.
6. According to the preparation method, the modified composite peptide C is designed, and is added with the GTCC for mixing, so that the moisturizing property of the modified composite peptide C is further improved, and the GTCC also has the function of protecting the structure of the modified composite peptide C.
7. According to the preparation method, the slow mixing speed of the modified composite peptide C and the premix D is designed to be 20-40 r/min, the time is 1-3 min, and in order to protect the cohesiveness of hydroxyethyl carboxymethyl cellulose on the surface of the peptide, a large amount of hydroxyethyl carboxymethyl cellulose coating film is dissolved in water as much as possible, and the slow release effect is lost.
In conclusion, because mites repeatedly enter and exit pores and can definitely carry a large amount of bacteria, when the mites enter and exit the pores and climb on the face, the carried bacteria can cause skin inflammation to cause acnes. Also, mite secretions can clog hair follicles, and hardening inflammation in hair follicles can also lead to the occurrence of vaccinia. The antibacterial and mite-removing cream disclosed by the invention is designed and prepared from the comprehensive angles of sterilizing and removing mites, inhibiting bacterial growth for a long time, regulating skin allergy resistance and improving skin immunity, so that a product with good antibacterial and mite-removing effects can be obtained, the antibacterial effect of 48 hours can be achieved, and the antibacterial and mite-removing cream has good practicability.
Detailed Description
The invention will be further illustrated with reference to specific examples, but the invention is not limited to these examples.
Examples
The antibacterial and mite-killing cream comprises, by mass, 2 parts of frog skin antibacterial peptide Dermaseptin, 0.02 part of Cecropin B,4 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 0.8 part of frog skin peptide Bombesin, 0.4 part of conotoxin peptide, 1.2 parts of tea polyphenol, 0.5 part of kaki pepper extract, 6 parts of plant extract, 0.3 part of hydroxyethyl carboxymethyl cellulose, 0.2 part of sodium hyaluronate, 0.3 part of butanediol, 0.2 part of triglyceride, 4 parts of GTCC, 0.5 part of ceramide and 10 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is 4:0.8:1.5.
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.15 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 40 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 2min at 30r/min at 40 ℃, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.15 part by weight of hydroxyethyl carboxymethyl cellulose uniformly at the temperature of 85 ℃, then cooling to 50 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously uniformly mixing, then cooling to 40 ℃, adding the premix D, and slowly mixing for 2min at a speed of 30r/min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 2
The antibacterial mite-killing cream comprises, by mass, 1.2 parts of frog skin antibacterial peptide Dermaseptin, 0.04 part of Cecropin B,3 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 0.6 part of frog skin antibacterial peptide Bombesin, 0.4 part of conotoxin peptide, 0.8 part of tea polyphenol, 0.3 part of kaki pepper extract, 7 parts of plant extract, 0.2 part of hydroxyethyl carboxymethyl cellulose, 0.2 part of sodium hyaluronate, 0.2 part of butanediol, 0.2 part of triglyceride, 3.5 parts of GTCC, 0.3 part of ceramide and 8 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts=3.5:0.6:1.2.
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.1 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 38 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 38deg.C to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 35 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 38 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 1min at 40r/min at 38 ℃, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.1 part of hydroxyethyl carboxymethyl cellulose uniformly at 80 ℃, then cooling to 45 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously mixing uniformly, then cooling to 38 ℃, adding the premix D, and mixing at a slow speed of 40r/min for 1min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 3
The antibacterial mite-killing cream comprises, by mass, 2.5 parts of frog skin antibacterial peptide Dermaseptin, 0.03 part of Cecropin B,5 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 0.8 part of frog skin antibacterial peptide Bombesin, 0.45 part of conotoxin peptide, 1.5 parts of tea polyphenol, 0.8 part of kaki pepper extract, 8 parts of plant extract, 0.4 part of hydroxyethyl carboxymethyl cellulose, 0.4 part of sodium hyaluronate, 0.4 part of butanediol, 0.4 part of triglyceride, 5 parts of GTCC, 0.8 part of ceramide and 18 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts=4.5:0.8:1.8.
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.2 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 38 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 3min at 20r/min at 40 ℃, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.2 part by weight of hydroxyethyl carboxymethyl cellulose uniformly at 83 ℃, then cooling to 50 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously uniformly mixing, then cooling to 40 ℃, adding the premix D, and slowly mixing for 3min at 20r/min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 4
The antibacterial and mite-killing cream comprises, by mass, 1 part of frog skin antibacterial peptide Dermaseptin, 0.02 part of Cecropin B,2 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 0.5 part of frog skin antibacterial peptide Bombesin, 0.3 part of conotoxin peptide, 0.5 part of tea polyphenol, 0.1 part of kaki pepper extract, 5 parts of plant extract, 0.1 part of hydroxyethyl carboxymethyl cellulose, 0.1 part of sodium hyaluronate, 0.1 part of butanediol, 0.1 part of triglyceride, 3 parts of GTCC, 0.1 part of ceramide and 5 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is (3:0.5:1).
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.05 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 35 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 1min at 40 ℃ at 40r/min, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.05 part of hydroxyethyl carboxymethyl cellulose uniformly at 80 ℃, then cooling to 45 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously mixing uniformly, then cooling to 40 ℃, adding the premix D, and mixing at a slow speed of 40r/min for 1min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 5
The antibacterial mite-killing cream comprises, by mass, 3 parts of frog skin antibacterial peptide Dermaseptin, 0.05 part of Cecropin B,6 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 1 part of frog skin peptide Bombesin, 0.5 part of conotoxin peptide, 2 parts of tea polyphenol, 1 part of kaki pepper extract, 10 parts of plant extract, 0.5 part of hydroxyethyl carboxymethyl cellulose, 0.5 part of sodium hyaluronate, 0.5 part of butanediol, 0.5 part of triglyceride, 6 parts of GTCC, 1 part of ceramide and 20 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is (5:1:2).
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.25 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 40 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 2min at 30r/min at 40 ℃, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.25 part of hydroxyethyl carboxymethyl cellulose uniformly at the temperature of 85 ℃, then cooling to 45 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously mixing uniformly, then cooling to 40 ℃, adding the premix D, and mixing at a slow speed of 30r/min for 2min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 6
The antibacterial mite-killing cream comprises, by mass, 1 part of frog skin antibacterial peptide Dermaseptin, 0.04 part of Cecropin B,2 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 1 part of frog skin peptide Bombesin, 0.3 part of conotoxin peptide, 2 parts of tea polyphenol, 0.1 part of kaki pepper extract, 10 parts of plant extract, 0.1 part of hydroxyethyl carboxymethyl cellulose, 0.5 part of sodium hyaluronate, 0.1 part of butanediol, 0.5 part of triglyceride, 3 parts of GTCC, 1 part of ceramide and 10 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is (3:0.5:2).
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.05 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 39 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 39deg.C to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 42 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 39 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 3min at 20r/min at 39 ℃, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.05 part of hydroxyethyl carboxymethyl cellulose uniformly at the temperature of 82 ℃, then cooling to 46 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously mixing uniformly, cooling to 39 ℃, adding the premix D, and mixing slowly for 3min at 20r/min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the antibacterial detection result, the peptide components and the plant extract are matched for use, the antibacterial effect can be greatly improved by the synergistic effect, the antibacterial effect of independently adding the peptide or extracting the plant is not ideal, and the antibacterial capability is greatly weakened. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
Example 7
The antibacterial mite-killing cream comprises, by mass, 3 parts of frog skin antibacterial peptide Dermaseptin, 0.01 part of Cecropin B,6 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide 1, 0.5 part of frog skin antibacterial peptide Bombesin, 0.5 part of conotoxin peptide, 0.5 part of tea polyphenol, 1 part of kaempferin, 5 parts of plant extract, 0.5 part of hydroxyethyl carboxymethyl cellulose, 0.1 part of sodium hyaluronate, 0.5 part of butanediol, 0.1 part of triglyceride, 6 parts of GTCC, 0.1 part of ceramide and 8.5 parts of purified water.
The plant extracts comprise sage extracts, burdock extracts and immature bitter orange extracts, wherein the mass ratio of the sage extracts to the burdock extracts to the immature bitter orange extracts is (5:1:2).
The preparation method of the antibacterial mite-killing cream comprises the following steps:
s1: according to the weight portion ratio, 0.25 part of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature of 36 ℃, and then lyophilizing with liquid nitrogen to obtain modified composite peptide C;
s2, respectively preheating the modified composite peptide C and the GTCC to 40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 2min at 40 ℃ and 40r/min, and preserving heat to obtain a premix D;
s3: mixing the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the residual 0.25 part of hydroxyethyl carboxymethyl cellulose uniformly at the temperature of 85 ℃, then cooling to 50 ℃, sequentially adding the conotoxin peptide, the tea polyphenol, the kaki peppers and the plant extract, continuously mixing uniformly, cooling to 40 ℃, adding the premix D, and mixing at a slow speed of 40r/min for 2min to obtain the antibacterial and anti-mite cream.
Experiment one, bacteriostasis experiment: the antibacterial and mite-removing cream prepared by the method is subjected to antibacterial experimental detection, and the test strains are propionibacterium acnes, candida albicans, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli and malassezia furfur.
Separately adding 100 mu L of bacterial liquid of each strain into micropores of a microporous plate, then adding 50 mu L of antibacterial and anti-mite cream, simultaneously preparing a blank sample without adding bacteria and a control sample without adding antibacterial and anti-mite cream, preparing 3 parallel samples for each sample, and culturing for 24 hours according to the culture temperature of each strain, wherein the detection result is as follows:
comparative example 1: the antibacterial mite-removing cream is not added with peptide components, and the detection result is as follows:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
as shown in the antibacterial detection result, the peptide component and the plant extract are matched for use, the synergistic effect can greatly improve the antibacterial effect, the antibacterial effect of independently adding peptide or extracting plant is not ideal, and the antibacterial capability is greatly reduced. The antibacterial and mite-removing cream prepared by the embodiment has very obvious antibacterial effect on propionibacterium acnes.
Experiment two, mite removal experiment: the antibacterial and mite-killing cream prepared by the method of the embodiment is subjected to mite-killing experimental detection, and the test mites are dust mites and Ruo mites.
The antibacterial mite-killing cream is respectively applied to 100 dust mites and 100 mites, three groups of parallel samples are made, and the mite mortality rate is observed, and the result is as follows:
comparative example 1: the antibacterial mite-removing cream does not contain conotoxin peptide, tea polyphenol and kaempferin, and has the following detection results:
comparative example 2: the antibacterial mite-removing cream is not added with plant extract components, and the detection result is as follows:
according to the mite removal detection result, the plant extract and conotoxin peptide, tea polyphenol and kaempferin are matched for use, so that the mite removal effect is good; the mite-removing effect of the components without adding plant extracts or conotoxin peptide, tea polyphenol and kaempferide is greatly reduced.
Experiment three, antibacterial and mite-inhibiting aging experiment:
the antibacterial and mite-killing cream prepared by the method is coated on a test piece (the coating thickness is transparent and free from accumulation) under the aseptic ring shape, and is naturally evaporated for 6h, 12h, 24h and 48h respectively, and then is placed in a bacteria mite-killing environment for 30min, and bacteria and mite adhesion conditions on the test piece are observed by a microscope, and the detection result is as follows:
comparative example 1: in the preparation process of the antibacterial and mite-removing cream, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin are directly mixed without coating hydroxyethyl carboxymethyl cellulose. The detection results are as follows:
The antibacterial and mite-inhibiting aging detection result shows that the cecropin and the anti-inflammatory peptide coated by the hydroxyethyl carboxymethyl cellulose are added into the antibacterial and mite-eliminating cream, so that the antibacterial and mite-inhibiting aging is longer, and the cleaning can be kept for a long time.
Experiment four, skin trial evaluation experiment:
selecting 20 subjects, and coating the antibacterial and mite-removing cream prepared in the embodiment once every day or every two days, wherein the coating part is facial skin and body skin, observing for 30 days, and the antibacterial and mite-removing cream has no skin itch and no new diseases caused by bacteria or mites; and the original symptoms are well improved.
The sage extract used in the above examples 1 to 7 was acetone extract of sage; the fructus Arctii extract is water extract of fructus Arctii; the fructus Aurantii Immaturus extract is methanol or ethanol extract of fructus Aurantii Immaturus. The plant extracts are all purchased raw materials.
The frog skin antibacterial peptide Dermaseptin is prepared from the following raw materials in sequence: ala-Leu-Trp-Lys-Thr-Met-Leu-Lys-Lys-Leu-Gly-Thr-Met-Ala-Leu-His-Ala-Gly-Lys-Ala-Ala-Leu-Gly-Ala-Ala-Ala-Asp-Thr-Ile-Ser-Gln-Gly-Thr-Gln.
The natural silk antibacterial peptide Cecropin B is prepared from the following raw materials in sequence: lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Met-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu-NH2.
The Anti-inflammatory peptide Anti-Inflammatory Peptide 1 is prepared from the following raw materials in sequence: met-Gln-Met-Lys-Lys-Val-Leu-Asp-Ser.
The Bombesin is prepared from the following raw materials in sequence: glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2.
The antibacterial and mite-killing cream of the embodiment 1 to the embodiment 7 is designed and prepared from the comprehensive angles of sterilizing and killing mites, inhibiting bacterial growth for a long time, regulating skin allergy resistance and improving skin immunity, so that a product with good antibacterial and mite-killing effects can reach the antibacterial effect of 48 hours, and has good practicability.
Claims (10)
1. The antibacterial and mite-killing cream is characterized by comprising, by mass, 1-3 parts of frog skin antibacterial peptide Dermaseptin, 0.01-0.05 part of Cecropin B, 2-6 parts of Anti-inflammatory peptide Anti-Inflammatory Peptide, 0.5-1 part of frog skin peptide Bombesin, 0.3-0.5 part of conotoxin peptide, 0.5-2 parts of tea polyphenol, 0.1-1 part of kaki pepper extract, 5-10 parts of plant extract, 0.1-0.5 part of hydroxyethyl carboxymethyl cellulose, 0.1-0.5 part of sodium hyaluronate, 0.1 part of butanediol, 0.1-0.5 part of triglyceride, 3-6 parts of GTCC, 0.1-1 part of ceramide and 5-20 parts of purified water.
2. The antibacterial and mite-killing cream according to claim 1, wherein the plant extracts comprise a sage extract, a burdock fruit extract and an immature bitter orange extract, and the mass ratio of the sage extract to the burdock fruit extract to the immature bitter orange extract is (3-5) = (0.5-1): (1-2).
3. The bacteriostatic mite-killing cream according to claim 2, wherein said sage extract is an acetone extract of sage.
4. The antibacterial and mite-killing cream according to claim 2, wherein the burdock fruit extract is an aqueous extract of burdock fruit.
5. The bacteriostatic and anti-mite cream according to claim 2, wherein the extract of citrus aurantium is a methanol or ethanol extract of citrus aurantium.
6. The method for preparing the antibacterial and mite-killing cream as claimed in claim 1, which is characterized by comprising the following steps:
s1: according to the weight portion ratio, 50% of hydroxyethyl carboxymethyl cellulose is dissolved in water, and the temperature is kept at 38-40 ℃ to obtain spray A; premixing dry powder of frog skin antibacterial peptide Dermaseptin, cecropin B, anti-inflammatory peptide Anti-Inflammatory Peptide 1 and Bombesin at 38-40 ℃ to obtain mixed peptide dry powder B; spraying spray A to the mixed peptide dry powder B by adopting a spray drying method or a fluidization spray method, coating hydroxyethyl carboxymethyl cellulose on the particle surfaces of the mixed peptide dry powder B, drying at a low temperature, and then freeze-drying to obtain modified composite peptide C;
S2, respectively preheating the modified composite peptide C and the GTCC to 38-40 ℃, then adding the GTCC into the modified composite peptide C, slowly mixing for 1-3 min at 38-40 ℃, and preserving heat to obtain a premix D;
s3: according to the mass portion ratio, the purified water, the sodium hyaluronate, the butanediol, the triglyceride, the ceramide and the rest 50% of hydroxyethyl carboxymethyl cellulose are uniformly mixed at the temperature of 80-85 ℃, then the temperature is reduced to 45-50 ℃, conotoxin peptide, tea polyphenol, kaempferide and plant extract are sequentially added, the mixture is continuously uniformly mixed, then the temperature is reduced to 38-40 ℃, the premix D is added, and the mixture is slowly mixed for 1-3 min, so that the antibacterial mite-removing cream is obtained.
7. The method for preparing the antibacterial and acaricidal cream according to claim 6, wherein in S1, the low-temperature drying temperature is not more than 45 ℃.
8. The method for preparing the antibacterial and acaricidal cream according to claim 6, wherein in the step S1, the freeze-drying is performed by liquid nitrogen.
9. The preparation method of the antibacterial and acaricidal cream according to claim 6, wherein in S2, the rotating speed of the slow mixing is 20-40 r/min.
10. The preparation method of the antibacterial and acaricidal cream according to claim 6, wherein in the step S3, the rotating speed of the slow mixing is 20-40 r/min.
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