CN117617242A - Sterilization composition containing d-limonene and allicin and application thereof - Google Patents
Sterilization composition containing d-limonene and allicin and application thereof Download PDFInfo
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- CN117617242A CN117617242A CN202311358188.2A CN202311358188A CN117617242A CN 117617242 A CN117617242 A CN 117617242A CN 202311358188 A CN202311358188 A CN 202311358188A CN 117617242 A CN117617242 A CN 117617242A
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- sheath blight
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- XMGQYMWWDOXHJM-JTQLQIEISA-N (+)-α-limonene Chemical compound CC(=C)[C@@H]1CCC(C)=CC1 XMGQYMWWDOXHJM-JTQLQIEISA-N 0.000 title claims abstract description 106
- JDLKFOPOAOFWQN-VIFPVBQESA-N Allicin Natural products C=CCS[S@](=O)CC=C JDLKFOPOAOFWQN-VIFPVBQESA-N 0.000 title claims abstract description 46
- JDLKFOPOAOFWQN-UHFFFAOYSA-N allicin Chemical compound C=CCSS(=O)CC=C JDLKFOPOAOFWQN-UHFFFAOYSA-N 0.000 title claims abstract description 46
- 235000010081 allicin Nutrition 0.000 title claims abstract description 46
- 239000000203 mixture Substances 0.000 title claims abstract description 28
- 230000001954 sterilising effect Effects 0.000 title claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 title claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 19
- 235000009566 rice Nutrition 0.000 claims abstract description 19
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 11
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 10
- 239000004480 active ingredient Substances 0.000 claims abstract description 5
- 201000010099 disease Diseases 0.000 claims description 19
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 19
- 241000209094 Oryza Species 0.000 claims description 18
- 239000000575 pesticide Substances 0.000 claims description 12
- 239000000839 emulsion Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002671 adjuvant Substances 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 239000004530 micro-emulsion Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims 3
- 241000227653 Lycopersicon Species 0.000 claims 2
- 239000006071 cream Substances 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 240000003768 Solanum lycopersicum Species 0.000 abstract description 9
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 30
- 238000012360 testing method Methods 0.000 description 25
- 230000000694 effects Effects 0.000 description 22
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 11
- 241000123650 Botrytis cinerea Species 0.000 description 8
- 239000004495 emulsifiable concentrate Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 244000052616 bacterial pathogen Species 0.000 description 6
- 230000009036 growth inhibition Effects 0.000 description 6
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 235000019482 Palm oil Nutrition 0.000 description 5
- 239000002540 palm oil Substances 0.000 description 5
- 244000052769 pathogen Species 0.000 description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 5
- 229920000053 polysorbate 80 Polymers 0.000 description 5
- 231100000419 toxicity Toxicity 0.000 description 5
- 230000001988 toxicity Effects 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000010413 mother solution Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000813090 Rhizoctonia solani Species 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 3
- 229940087305 limonene Drugs 0.000 description 3
- 235000001510 limonene Nutrition 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 231100000053 low toxicity Toxicity 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 235000020971 citrus fruits Nutrition 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000000611 regression analysis Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 240000002234 Allium sativum Species 0.000 description 1
- 241001290235 Ceratobasidium cereale Species 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000175448 Citrus madurensis Species 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 235000005976 Citrus sinensis Nutrition 0.000 description 1
- 240000002319 Citrus sinensis Species 0.000 description 1
- 240000000560 Citrus x paradisi Species 0.000 description 1
- 235000017317 Fortunella Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000233732 Fusarium verticillioides Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000024873 Mentha crispa Species 0.000 description 1
- 235000014749 Mentha crispa Nutrition 0.000 description 1
- 244000246386 Mentha pulegium Species 0.000 description 1
- 235000016257 Mentha pulegium Nutrition 0.000 description 1
- 235000004357 Mentha x piperita Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000191963 Staphylococcus epidermidis Species 0.000 description 1
- 241001617088 Thanatephorus sasakii Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 229930008380 camphor Natural products 0.000 description 1
- 244000000037 crop pathogen Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000010645 fir oil Substances 0.000 description 1
- -1 for example Substances 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 235000004611 garlic Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000001050 hortel pimenta Nutrition 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000000902 placebo Substances 0.000 description 1
- 229940068196 placebo Drugs 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a bactericidal composition containing d-limonene and allicin, wherein the active ingredients of the bactericidal composition are composed of d-limonene and allicin, and the mass ratio of the d-limonene to the allicin is 30:1-1:30. The d-limonene can be used for preventing and controlling rice sheath blight or tomato gray mold, and the d-limonene and the allicin have remarkable synergistic effect after being combined, and are safe for crops in field observation.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a sterilization composition containing d-limonene and allicin and application thereof.
Background
Chemical pesticides are dominant in pesticide markets due to the characteristics of multiple varieties, quick response, convenient use, low price and the like. However, due to the unreasonable use of chemical pesticides and the inherent defects of pesticides, the chemical pesticides cause the problems of environmental pollution, poisoning of people and livestock, pesticide residues and the like in use. With the improvement of the life quality and the enhancement of environmental awareness of people, the development of biological pesticides with safety, no pollution, low toxicity and low residue for people and livestock becomes a new hot spot for research in the pesticide field. Plant-derived agricultural chemicals have been one of the hot topics in the research of new agricultural chemicals as part of biologically rational agricultural chemicals. The botanical pesticide is derived from nature, can be degraded in nature, generally does not pollute the environment and agricultural products, has little possibility of accumulating toxicity in the environment and human bodies, is relatively safe to people and livestock, has little harm to natural enemies of pests, has the characteristics of low toxicity and low residue, can keep the high quality of the agricultural products, and therefore has wide market.
Limonene, also known as limonene, is a common component in plant essential oils, mainly found in citrus fruits such as citrus, sweet orange, lemon, orange, grapefruit, kumquat, and the like, and also contained in other plants such as peppermint, camphor, fir oil, pine needle, spearmint. d-limonene is the dextro-configurational isomer of limonene. There are relatively few studies of the agricultural bactericidal activity of d-limonene in the prior art.
Allicin is an ingredient extracted from bulb of garlic, is known as a natural broad-spectrum antibiotic, has been widely used for inhibiting staphylococcus aureus, staphylococcus albus and the like, and has been studied and reported to have a certain inhibiting effect on pathogenic fungi such as rhizoctonia solani, fusarium moniliforme, fusarium oxysporum wilt specialization fungi, rice blast germs, rhizoctonia cerealis, sclerotium bacteria, botrytis cinerea and the like.
The plant source sterilization component has the characteristics of small damage to natural enemies of pests, low toxicity and low residue, and can keep the high quality of agricultural products, but the sterilization activity of the plant source sterilization component is also relatively low, and the synergistic sterilization composition is required to be compounded so as to improve the sterilization activity.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the following technical scheme:
a bactericidal composition containing d-limonene and allicin comprises the active ingredients of d-limonene and allicin, wherein the mass ratio of the d-limonene to the allicin is 30:1-1:30. Preferably, the mass ratio of the d-limonene to the allicin is 25:1-1:5; further preferably, the mass ratio of the d-limonene to the allicin is 25:1-5:1.
The bactericidal composition contains pesticide auxiliary ingredients, and the active ingredients account for 1-30% of the bactericidal composition by weight percent. Preferably, the pesticide adjuvant comprises known adjuvants, such as, for example, emulsifiers, wetting agents, dispersants, organic solvents, co-solvents, water, preservatives, antifreezes and the like.
The preparation of the bactericidal composition is missible oil, microemulsion, aqueous emulsion and soluble liquid.
The bactericidal composition is used for preventing and controlling crop diseases, in particular to rice sheath blight or tomato gray mold.
The invention also provides an application of d-limonene in preventing and controlling rice sheath blight or tomato gray mold.
By adopting the technical scheme, the invention has the following beneficial effects:
less research on the prevention and treatment of crop pathogens by d-limonene, and research and development show that the d-limonene can be used for preventing and treating rice sheath blight or gray mold of tomato gray mold.
When the mass ratio of d-limonene to allicin is 25:1-1:5, the composition has excellent control effect on rice sheath blight germs and has remarkable synergy.
When the mass ratio of d-limonene to allicin is 25:1-5:1, the composition has excellent control effect on pathogens and obvious synergy.
The emulsifiable concentrate preparation prepared by the invention has excellent field control effect and is safe for crops in field observation.
Detailed Description
The invention is further illustrated with reference to specific embodiments.
Example 1: indoor toxicity determination of d-limonene and allicin on rice sheath blight pathogen
Test pathogen: rhizoctonia solani (Rhizoctonia solani).
The test method comprises the following steps:
preparing potato culture medium (PDA), weighing 200g potato, placing in 1000mL distilled water, boiling for 30min, filtering, adding 20g glucose and 20g agar, thawing, supplementing with distilled water to 1000mL, packaging, and sterilizing.
The hypha growth rate method was used for measurement. On the basis of preliminary tests, 7 concentrations of each medicament with the growth inhibition rate of the pathogenic bacteria hypha within the range of 10% -90% are selected, the medicaments are prepared into mother solution with a certain concentration, and the mother solution is sequentially prepared into medicament-containing culture mediums with a series of concentration gradients according to the concentration. A bacterial cake of the pathogenic bacteria to be used, which is cultured on the PDA culture medium, is intercepted by a puncher, hyphae are inoculated to the center of the solidified PDA culture medium containing the medicine in a face up mode, the PDA culture medium is cultured at room temperature, and when the diameter of a control colony grows to be 3/4 larger than that of a plate, the diameter (mm) of the colony is measured by a caliper. Each colony was measured vertically 1 time in diameter by the cross-over method, and the average value was taken. Each treatment was repeated 4 times. The effect of the test agent on the growth of the test pathogenic bacterial hypha was analyzed and compared. The test data were analyzed by regression analysis with the logarithmic value of the concentration of the drug as the abscissa and the several value of the hypha growth inhibition rate as the ordinate, and the EC50 value of each treatment was calculated.
Growth inhibition ratio (%) = (control colony diameter-treated colony diameter)/(control colony diameter-cake diameter) ×100
The synergy evaluation method comprises the following steps: the evaluation is carried out by adopting a co-toxicity coefficient method, wherein the co-toxicity coefficient standard is that CTC is more than or equal to 120 and is synergistic, CTC is more than or equal to 80 and is less than or equal to 120 and is additive, and CTC is more than or equal to 80 and is antagonistic.
Test results are shown in the following formula
The test results in Table 1 show that d-limonene has good inhibitory activity on the hypha growth of rice sheath blight bacteria, and the EC50 value is 215.60mg/L. Compared with d-limonene, the allicin has higher inhibition activity on the growth of rice sheath blight germ hyphae, and the EC50 value of the allicin is 35.48mg/L. After the d-limonene and the allicin are combined, the inhibition effect of the medicament on the growth of rice sheath blight germ hypha can be improved, and when the mass ratio of the d-limonene to the allicin is 25:1-1:5, the composition shows excellent prevention and control effects on germ, and compared with single use, the composition has excellent synergistic effect.
Table 1: indoor toxicity determination of d-limonene and allicin on rice sheath blight pathogen
Example 2: indoor toxicity determination of d-limonene and allicin on botrytis cinerea
Test pathogen: botrytis cinerea (Botrytis cinerea).
The test method comprises the following steps:
the hypha growth rate method was used for measurement. On the basis of preliminary tests, 7 concentrations of each medicament with the growth inhibition rate of the pathogenic bacteria hypha within the range of 10% -90% are selected, the medicaments are prepared into mother solution with a certain concentration, and the mother solution is sequentially prepared into medicament-containing culture mediums with a series of concentration gradients according to the concentration. A bacterial cake of the pathogenic bacteria to be used, which is cultured on the PDA culture medium, is intercepted by a puncher, hyphae are inoculated to the center of the solidified PDA culture medium containing the medicine in a face up mode, the PDA culture medium is cultured at room temperature, and when the diameter of a control colony grows to be 2/3 larger than that of a plate, the diameter (mm) of the colony is measured by a caliper. Each colony was measured vertically 1 time in diameter by the cross-over method, and the average value was taken. Each treatment was repeated 4 times. The effect of the test agent on the growth of the test pathogenic bacterial hypha was analyzed and compared. The test data were analyzed by regression analysis with the logarithmic value of the concentration of the drug as the abscissa and the several value of the hypha growth inhibition rate as the ordinate, and the EC50 value of each treatment was calculated.
Growth inhibition ratio (%) = (control colony diameter-treated colony diameter)/(control colony diameter-cake diameter) ×100
The synergy evaluation method comprises the following steps: the evaluation is carried out by adopting a co-toxicity coefficient method, wherein the co-toxicity coefficient standard is that CTC is more than or equal to 120 and is synergistic, CTC is more than or equal to 80 and is less than or equal to 120 and is additive, and CTC is more than or equal to 80 and is antagonistic.
Test results:
the test results in Table 2 show that d-limonene has good inhibition activity on the hypha growth of Botrytis cinerea, and the EC50 value of the d-limonene is 160.33mg/L. Compared with d-limonene, the allicin has higher inhibition activity on the growth of Botrytis cinerea hyphae, and the EC50 value of the allicin is 1.42mg/L. After the d-limonene and the allicin are combined, the inhibiting effect of the medicament on the growth of botrytis cinerea hyphae can be improved, and when the mass ratio of the d-limonene to the allicin is 25:1-5:1, the composition shows excellent control effect on bacteria, and compared with single use, the composition has excellent synergistic effect.
Table 2: indoor toxicity determination of d-limonene and allicin on botrytis cinerea
Example 3: test of field control effect of d-limonene and allicin on rice sheath blight disease
Crop disease: sheath blight of rice
Testing the medicament: self-made medicament 1-5.
Medicament 1:10% d-limonene allicin emulsifiable concentrate
8% of d-limonene, 2% of allicin, 600#6% of agro-emulsion, 4% of Tween 80, 4% of glycol and 100% of palm oil. All the above are weight percentages.
2:11% d-limonene allicin emulsifiable concentrate
10% of d-limonene, 1% of allicin, 600#4% of agro-emulsion, 3% of Tween 80, 3% of glycol and 100% of palm oil. All the above are weight percentages.
Medicament 3: 8%d-limonene allicin emulsifiable concentrate
4% of d-limonene, 4% of allicin, 600#5% of agro-emulsion, 2% of Tween 80, 3% of glycol and 100% of palm oil. All the above are weight percentages.
4:4% d-limonene emulsifiable concentrate
4% of d-limonene, 600#5% of agro-emulsion, 2% of Tween 80, 3% of ethylene glycol and 100% of palm oil. All the above are weight percentages.
Medicament 5:4% allicin emulsifiable concentrate
4% of allicin, 600#5% of agro-emulsion, 2% of tween 80, 3% of glycol and 100% of palm oil. All the above are weight percentages.
The testing method comprises the following steps: drug treatment groups and control groups were set, 4 replicates, randomized block arrangement, cell area 45m2. In the tillering stage of rice, spraying medicine to stem and leaf for 1 time, the dosage of the medicine liquid is 450kg/hm2, the disease number is investigated before the medicine is applied, and the disease index is investigated for calculating the control effect after 14d after the medicine is applied.
Disease classification standard of rice sheath blight:
level 0: the whole plant is free from diseases;
stage 1: fourth leaf and leaf sheath and leaf onset below (sword leaf is used as first leaf);
3 stages: a third leaf and leaf sheaths and leaf attacks below the third leaf;
5 stages: a second leaf and leaf sheaths and leaf attacks below the second leaf;
7 stages: sword leaf blade and leaf sheaths below the blade are used for disease occurrence;
stage 9: the whole plant is ill and die early.
The calculation formula is as follows:
test results are shown in the specification
The test results in Table 3 show that the d-limonene allicin emulsifiable concentrate prepared by the invention has excellent field control effect on rice sheath blight. Compared with the independent use of the medicament 4 and the medicament 5, the medicament composition can obviously improve the control effect on the rice sheath blight disease and has obvious synergy; and the field observation is safe to crops.
Table 3: test of field control effect of d-limonene and allicin on rice sheath blight disease
Example 4: d-limonene and allicin field control effect test for tomato gray mold
Crop disease: gray mold of tomato
Testing the medicament: in example 3, agents 1-5 were prepared.
The test method comprises the following steps: drug treatment and control groups were set, 4 replicates, randomized block arrangement, cell area 30m2. Spraying stem and leaf to make the medicinal liquid sprayed on the front and back sides of the leaf; the application was twice at 7d intervals. The disease grade of the leaves was investigated before the 1 st dose and after the 2 nd dose for 7 d. The disease classification criteria are:
stage 1: no disease spots
3 stages: single leaf with 3 lesions
5 stages: 4-6 single leaf spots
7 stages: 7-10 single leaf spots
Stage 9: the single leaf has disease spots which occupy more than one quarter of the leaf area densely.
The drug effect calculation formula:
wherein: CK (CK) o -a blank control zone pre-dose index;
CK 1 -disease index after administration in the placebo area;
PT o -an index of pre-dose condition at the dose treatment zone;
PT 1 -index of disease after administration in the agent treatment zone.
Test results:
the test results in Table 4 show that the d-limonene allicin emulsifiable concentrate prepared by the invention has excellent field control effect on tomato gray mold. Compared with the independent use of the medicament 4 and the medicament 5, the medicament composition can obviously improve the control effect on the gray mold of the tomatoes and has obvious synergy; and the field observation is safe to crops.
Table 4: d-limonene and allicin field control effect test for tomato gray mold
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same.
Claims (7)
1. A sterilization composition containing d-limonene and allicin is characterized in that the active ingredients consist of d-limonene and allicin, and the mass ratio of the d-limonene to the allicin is 30:1-1:30.
2. The bactericidal composition of claim 1, wherein the mass ratio of d-limonene to allicin is 25:1-1:5.
3. The bactericidal composition of claim 2, wherein the mass ratio of d-limonene to allicin is 25:1-5:1.
4. A fungicidal composition according to any one of claims 1-3, comprising a pesticide adjuvant ingredient, the active ingredient being present in an amount of 1-30% by weight of the fungicidal composition.
5. The composition of claim 4, wherein the formulation of the composition is a cream, a microemulsion, an aqueous emulsion, or a soluble liquid.
6. Use of a fungicidal composition according to any one of claims 1-5 for controlling crop diseases, wherein the disease is rice sheath blight or tomato gray mold.
Use of d-limonene for controlling rice sheath blight or tomato gray mold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311358188.2A CN117617242A (en) | 2023-10-19 | 2023-10-19 | Sterilization composition containing d-limonene and allicin and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311358188.2A CN117617242A (en) | 2023-10-19 | 2023-10-19 | Sterilization composition containing d-limonene and allicin and application thereof |
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| Publication Number | Publication Date |
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| CN117617242A true CN117617242A (en) | 2024-03-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311358188.2A Pending CN117617242A (en) | 2023-10-19 | 2023-10-19 | Sterilization composition containing d-limonene and allicin and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117617242A (en) |
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2023
- 2023-10-19 CN CN202311358188.2A patent/CN117617242A/en active Pending
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