CN117598403A - Hyperbranched polylysine-starch compound-based pet diet agent and preparation method thereof - Google Patents
Hyperbranched polylysine-starch compound-based pet diet agent and preparation method thereof Download PDFInfo
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- CN117598403A CN117598403A CN202311761877.8A CN202311761877A CN117598403A CN 117598403 A CN117598403 A CN 117598403A CN 202311761877 A CN202311761877 A CN 202311761877A CN 117598403 A CN117598403 A CN 117598403A
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- starch
- pet
- hyperbranched polylysine
- polylysine
- hyperbranched
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- 229920002472 Starch Polymers 0.000 title claims abstract description 54
- 239000008107 starch Substances 0.000 title claims abstract description 54
- 235000005911 diet Nutrition 0.000 title claims abstract description 23
- 230000037213 diet Effects 0.000 title claims abstract description 23
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 21
- 150000001875 compounds Chemical class 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 235000019698 starch Nutrition 0.000 claims abstract description 26
- 108010039918 Polylysine Proteins 0.000 claims abstract description 25
- 229920000656 polylysine Polymers 0.000 claims abstract description 25
- 230000000968 intestinal effect Effects 0.000 claims abstract description 11
- 208000008589 Obesity Diseases 0.000 claims abstract description 8
- 235000020824 obesity Nutrition 0.000 claims abstract description 8
- 230000000694 effects Effects 0.000 claims abstract description 7
- 230000036039 immunity Effects 0.000 claims abstract description 5
- 230000009471 action Effects 0.000 claims abstract description 4
- 235000013305 food Nutrition 0.000 claims description 26
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 235000021434 dietary agent Nutrition 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000003993 interaction Effects 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims 1
- 239000003002 pH adjusting agent Substances 0.000 claims 1
- 230000008859 change Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000003833 bile salt Substances 0.000 abstract description 2
- 239000000839 emulsion Substances 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 235000019626 lipase activity Nutrition 0.000 abstract description 2
- 229950004354 phosphorylcholine Drugs 0.000 abstract description 2
- PYJNAPOPMIJKJZ-UHFFFAOYSA-N phosphorylcholine chloride Chemical compound [Cl-].C[N+](C)(C)CCOP(O)(O)=O PYJNAPOPMIJKJZ-UHFFFAOYSA-N 0.000 abstract description 2
- 210000000813 small intestine Anatomy 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 description 8
- 230000036541 health Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 210000000987 immune system Anatomy 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 235000012631 food intake Nutrition 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 230000005802 health problem Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000012659 Joint disease Diseases 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 235000014105 formulated food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a pet diet agent based on hyperbranched polylysine-starch compound and a preparation method thereof. Wherein hyperbranched polylysine (HBPL) and starch generate hyperbranched polylysine-starch complex through electrostatic action. The pet diet agent can effectively decompose emulsion containing bile salt and phosphorylcholine, inhibit lipase activity, reduce fat absorption of small intestine, achieve the effect of inhibiting obesity of pets, and the polylysine-starch compound can obviously change intestinal flora structure, so that the immunity of pets can be improved.
Description
Technical Field
The invention relates to a pet diet agent based on hyperbranched polylysine-starch compound and a preparation method thereof, belonging to the field of pet health products.
Background
With the improvement of living standard, people are increasingly concerned about the health of pets. The problem of obesity in pets is becoming more and more common in today's society, which not only negatively affects the quality of life of the pets, but may also lead to serious health risks including joint diseases, diabetes, heart diseases, etc. One of the main causes of obesity in pets is excessive energy intake and insufficient exercise. During pet feeding, it is often difficult for the feeder to control the food intake of the pet, and high energy, high fat foods are often selected, which results in excessive calories ingested by the pet. It is therefore desirable to provide a dietary agent that can meet the basic nutritional needs of pets, while helping them to control body weight. Currently, there are some products on the market for managing the weight of pets, but their effectiveness and safety are different. Some products may rely too much on limiting pet food intake, and ignore providing comprehensive nutrition. Other products may contain inappropriate additives or ingredients that can negatively impact the health of the pet.
Meanwhile, the immunity of the pets is critical to the overall health of the pets. The intestinal flora of pets plays an important role in the immune system. Scientific research shows that intestinal flora is closely related to the immune system of pets, and that imbalance of flora may lead to reduced immune system function, which makes pets vulnerable to various health problems.
The present invention aims to address obesity and immune health problems in pets by providing a safe, non-toxic and effective diet for pets, which provides a reliable means for pet breeders to help the pet breeders maintain the desired weight of the pet and to strengthen their immune system.
Disclosure of Invention
The invention aims to provide a pet diet agent which has comprehensive effects, can inhibit obesity of pets, and can obviously change the intestinal flora structure of the pets so as to improve the immunity of the pets. The diet comprises a series of natural, gut flora friendly ingredients which help control the weight of the pet, promote growth of beneficial bacteria and inhibit the proliferation of harmful bacteria. By dietary supplementation, the pet can more easily maintain healthy intestinal flora balance, thereby improving the vitality and resistance of the immune system.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a pet dietary agent based on hyperbranched polylysine-starch complex, the pet dietary agent consisting of hyperbranched polylysine (HBPL) and starch; the dietary agent can be directly added into pet food to play roles in losing weight and balancing intestinal flora. The preparation method of the pet diet agent comprises the steps of dissolving hyperbranched polylysine and starch in water (usually, starch can be dissolved in aqueous solution firstly, hyperbranched polylysine is added after the starch is uniformly mixed, stirring is uniform), stirring and reacting for 12-24 hours at room temperature, adding a pH regulator to regulate the pH of the solution, and freeze-drying after the reaction is completed to obtain the hyperbranched polylysine-starch compound pet diet agent.
The applicable pH range of the solution is 6-8.
The mass ratio of the hyperbranched polylysine to the starch is 1:100-1:500.
the electrostatic action is the interaction of positively charged hyperbranched polylysine and negatively charged starch.
The pH regulator is one or more of citric acid, acetic acid and phosphoric acid.
The beneficial effects of the invention are that
1. The hyperbranched polylysine-starch compound pet dietary agent prepared by the invention can be directly added into pet food, can effectively decompose emulsion containing bile salt and phosphorylcholine, further inhibit lipase activity, reduce absorption of fat by the small intestine, achieve the effect of inhibiting obesity of pets, and can obviously change the intestinal flora structure and improve the immunity of pets.
2. The pet dietary agent of the hyperbranched polylysine-starch compound prepared by the invention has extremely strong lipase inhibition effect, so that the required dosage is less.
3. The hyperbranched polylysine-starch compound pet diet agent prepared by the invention has simple and safe preparation process, no side effect, degradation products of lysine and glucose are necessary for human body, and the preparation process is nontoxic and beneficial to health.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1
The preparation method of the pet diet agent of the embodiment comprises the following steps:
adding hyperbranched polylysine and starch into a proper amount of deionized water (the starch is dissolved before the hyperbranched polylysine is added), wherein the mass ratio of the hyperbranched polylysine to the starch is 1:100, stirring for 12h, regulating the pH to 7.0 by using citric acid, and freeze-drying to obtain the hyperbranched polylysine-starch compound. 1 part of hyperbranched polylysine-starch compound and 100 parts of pet food. The raw materials are sequentially weighed according to the weight parts and fully mixed, and the pet weight-losing food is prepared.
Example 2
Adding hyperbranched polylysine and starch into a proper amount of deionized water (the starch is dissolved before the hyperbranched polylysine is added), wherein the mass ratio of the hyperbranched polylysine to the starch is 1:200, stirring for 12h, regulating the pH to 6.0 by using citric acid, and freeze-drying to obtain the hyperbranched polylysine-starch compound. 1 part of hyperbranched polylysine-starch compound and 50 parts of pet food. The raw materials are sequentially weighed according to the weight parts and fully mixed, and the pet weight-losing food is prepared.
Example 3
Adding hyperbranched polylysine and starch into a proper amount of deionized water (the starch is dissolved before the hyperbranched polylysine is added), wherein the mass ratio of the hyperbranched polylysine to the starch is 1:300, stirring for 12h, regulating the pH to 8.0 by using citric acid, and freeze-drying to obtain the hyperbranched polylysine-starch compound. 1 part of hyperbranched polylysine-starch compound and 33 parts of pet food. The raw materials are sequentially weighed according to the weight parts and fully mixed, and the pet weight-losing food is prepared.
Example 4
Adding hyperbranched polylysine and starch into a proper amount of deionized water (the starch is dissolved before the hyperbranched polylysine is added), wherein the mass ratio of the hyperbranched polylysine to the starch is 1:400, stirring for 18h, regulating the pH to 6.5 by using citric acid, and freeze-drying to obtain the hyperbranched polylysine-starch compound. 1 part of hyperbranched polylysine-starch compound and 25 parts of pet food. The raw materials are sequentially weighed according to the weight parts and fully mixed, and the pet weight-losing food is prepared.
Example 5
Adding hyperbranched polylysine and starch into a proper amount of deionized water (the starch is dissolved before the hyperbranched polylysine is added), wherein the mass ratio of the hyperbranched polylysine to the starch is 1:500, stirring for 24h, regulating pH to 7.5 with citric acid, and lyophilizing to obtain hyperbranched polylysine-starch complex. 1 part of hyperbranched polylysine-starch compound and 20 parts of pet food. The raw materials are sequentially weighed according to the weight parts and fully mixed, and the pet weight-losing food is prepared.
Comparative example 1
This comparative example is similar to examples 1-5, except that the hyperbranched polylysine-starch complex is removed from the pet food.
30 healthy adult beagle dogs are fed with high-fat feed (crude protein is more than or equal to 30 percent, crude fat is more than or equal to 20 percent) continuously for 4 weeks to perform high-fat obesity modeling. After successful modeling, dividing into 6 groups according to body weight at random, wherein each group comprises 5 healthy dogs; wherein, 5 groups of pet foods added with the pet diet agents of examples 1-5 are respectively fed during feeding, the other group of pet foods are only fed by taking comparative example 1 as a control group, the pet diet agents are not added, the feeding dosages of the weight-reducing compositions in all the groups of pet foods are 10 g/kg.BW/d, the feeding modes are completely the same, the feeding amounts of basic dog foods in all the groups of pet weight-reducing foods are the same, and the continuous feeding is carried out for 8 weeks.
Weighing was carried out once every four weeks before the start of the test, after the successful molding and during the test period, and the results are shown in Table 1.
TABLE 1 influence of the dietary agents of pets on body weight
| Week 0 | Week 4 | Week 8 | Week 12 | |
| Example 1 | 10.23±1.21 a | 12.87±1.68 b | 11.46±1.20 b | 10.16±1.90 a |
| Example 2 | 10.43±1.34 a | 12.67±1.70 b | 11.77±1.69 b | 11.10±1.78 a |
| Example 3 | 10.35±1.67 a | 12.86±1.43 b | 11.80±1.67 b | 10.93±1.59 a |
| Example 4 | 10.67±1.73 a | 13.01±1.45 b | 12.11±1.60 b | 11.22±1.30 a |
| Example 5 | 10.56±1.25 a | 12.78±1.78 b | 11.87±1.56 b | 11.13±1.70 a |
| Comparative example 1 | 10.64±1.45 a | 12.89±1.89 a | 13.45±1.28 a | 14.56±1.63 a |
Note that: a: the difference is not significant (P > 0.05), b: significant difference (P < 0.05), weight unit of pet kg
As can be seen from table 1:
1. the weight of the plastic film is obviously increased (P is less than 0.05) after 4 weeks of test, which shows that the molding is successful;
2. examples 1-5 have a significant difference in body weight (P < 0.05) from week 4 of the test, indicating that the pet dietary formulations of examples 1-5 have good weight loss effects;
3. the weight of the pet food in comparative example 1 is not significantly different from the weight of the pet food in the 4 th week of the test (P is more than 0.05), and the weight of the pet food in the pet food is not increased, so that the pet food has a significant weight-losing effect when the diet agent is added.
Intestinal flora assay
Intestinal beneficial flora measurements were made on 6 groups of healthy dogs, respectively, before feeding, and the average value of beneficial flora was recorded for each group, followed by a second measurement and recording after 4 weeks of the test, a third measurement and recording after 8 weeks of the test, and a fourth measurement and recording after 12 weeks of the test, with the results shown in table 2.
Table 2 test of the number of flora at week 0, week 4, week 8 and week 12
Note that: a: p <0.05 compared to comparative example 1.
The results in Table 2 show that after 4 weeks of the test, the beneficial flora in the intestinal tract of the pet is reduced to a certain extent, and the hyperbranched polylysine-starch compound of examples 1-5 is added to increase the beneficial flora in the intestinal tract of the pet after 12 weeks of the test, which indicates that the hyperbranched polylysine-starch compound is added to the food for the pet, so that the intestinal tract health of the pet can be protected, the intestinal flora can be stabilized, the growth of the beneficial flora in the intestinal tract can be promoted, and the immune system efficacy in the pet can be enhanced.
Claims (7)
1. A pet diet agent based on hyperbranched polylysine-starch complex, characterized in that the pet diet agent comprises hyperbranched polylysine (HBPL) and starch, which are hyperbranched polylysine-starch complex generated by electrostatic action.
2. The hyperbranched polylysine-starch complex-based pet dietary agent of claim 1 where the pet dietary agent is used as a direct addition to pet food to inhibit obesity in pets while altering the intestinal flora structure to enhance immunity in pets.
3. The pet diet agent according to claim 1, which is prepared by the following preparation method: dissolving starch in an aqueous solution, adding hyperbranched polylysine after uniformly mixing, uniformly stirring, preparing a hyperbranched polylysine-starch compound through electrostatic action, reacting for 12-24 hours at room temperature, and adding a pH regulator; and freeze-drying after the reaction is completed, thus obtaining the pet diet agent.
4. The pet diet agent according to claim 3, wherein the mass ratio of the hyperbranched polylysine to the starch is 1:100-1:500.
5. a pet diet agent according to claim 3 wherein the electrostatic effect is interaction of positively charged hyperbranched polylysine with negatively charged starch.
6. A pet diet agent in accordance with claim 3, wherein the pH of said solution is in the range of 6-8.
7. A pet diet agent according to claim 3, wherein the pH adjuster is one or more of citric acid, acetic acid and phosphoric acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311761877.8A CN117598403A (en) | 2023-12-20 | 2023-12-20 | Hyperbranched polylysine-starch compound-based pet diet agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311761877.8A CN117598403A (en) | 2023-12-20 | 2023-12-20 | Hyperbranched polylysine-starch compound-based pet diet agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117598403A true CN117598403A (en) | 2024-02-27 |
Family
ID=89956274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311761877.8A Pending CN117598403A (en) | 2023-12-20 | 2023-12-20 | Hyperbranched polylysine-starch compound-based pet diet agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117598403A (en) |
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2023
- 2023-12-20 CN CN202311761877.8A patent/CN117598403A/en active Pending
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