CN117511553A - Soil conditioner for promoting growth of capsicum and preparation method thereof - Google Patents
Soil conditioner for promoting growth of capsicum and preparation method thereof Download PDFInfo
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- CN117511553A CN117511553A CN202311490099.3A CN202311490099A CN117511553A CN 117511553 A CN117511553 A CN 117511553A CN 202311490099 A CN202311490099 A CN 202311490099A CN 117511553 A CN117511553 A CN 117511553A
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- soil
- soil conditioner
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- 239000003516 soil conditioner Substances 0.000 title claims abstract description 65
- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 230000001737 promoting effect Effects 0.000 title claims abstract description 38
- 239000001390 capsicum minimum Substances 0.000 title claims abstract description 11
- 240000008574 Capsicum frutescens Species 0.000 title claims abstract 3
- 239000002245 particle Substances 0.000 claims abstract description 61
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000003860 storage Methods 0.000 claims abstract description 30
- 230000000813 microbial effect Effects 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 18
- 240000008042 Zea mays Species 0.000 claims abstract description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 13
- 235000005822 corn Nutrition 0.000 claims abstract description 13
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 8
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 8
- 239000000853 adhesive Substances 0.000 claims abstract description 6
- 230000001070 adhesive effect Effects 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 5
- 235000016709 nutrition Nutrition 0.000 claims abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 41
- 230000004151 fermentation Effects 0.000 claims description 41
- 239000007788 liquid Substances 0.000 claims description 30
- 239000006002 Pepper Substances 0.000 claims description 28
- 241000722363 Piper Species 0.000 claims description 28
- 235000016761 Piper aduncum Nutrition 0.000 claims description 28
- 235000017804 Piper guineense Nutrition 0.000 claims description 28
- 235000008184 Piper nigrum Nutrition 0.000 claims description 28
- 238000002156 mixing Methods 0.000 claims description 26
- 238000001035 drying Methods 0.000 claims description 25
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 20
- 239000002609 medium Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- 241000758706 Piperaceae Species 0.000 claims description 19
- 241001608472 Bifidobacterium longum Species 0.000 claims description 15
- 241000186226 Corynebacterium glutamicum Species 0.000 claims description 15
- 240000001929 Lactobacillus brevis Species 0.000 claims description 15
- 235000013957 Lactobacillus brevis Nutrition 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 229940009291 bifidobacterium longum Drugs 0.000 claims description 15
- 239000007921 spray Substances 0.000 claims description 15
- 238000007873 sieving Methods 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 10
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 10
- 238000000498 ball milling Methods 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 10
- 238000000576 coating method Methods 0.000 claims description 10
- 238000001816 cooling Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- NQGIJDNPUZEBRU-UHFFFAOYSA-N dodecanoyl chloride Chemical compound CCCCCCCCCCCC(Cl)=O NQGIJDNPUZEBRU-UHFFFAOYSA-N 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 10
- 239000002244 precipitate Substances 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 238000012216 screening Methods 0.000 claims description 8
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 6
- 241000222290 Cladosporium Species 0.000 claims description 6
- 239000004113 Sepiolite Substances 0.000 claims description 6
- 229910021536 Zeolite Inorganic materials 0.000 claims description 6
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 6
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 6
- 235000019355 sepiolite Nutrition 0.000 claims description 6
- 229910052624 sepiolite Inorganic materials 0.000 claims description 6
- 239000010457 zeolite Substances 0.000 claims description 6
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 claims description 5
- 239000012880 LB liquid culture medium Substances 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 238000001354 calcination Methods 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 5
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 229940014800 succinic anhydride Drugs 0.000 claims description 5
- 239000005720 sucrose Substances 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 239000000440 bentonite Substances 0.000 claims description 4
- 229910000278 bentonite Inorganic materials 0.000 claims description 4
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000011230 binding agent Substances 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000009423 ventilation Methods 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 90
- 230000035699 permeability Effects 0.000 abstract description 11
- 230000006872 improvement Effects 0.000 abstract description 9
- 150000003839 salts Chemical class 0.000 abstract description 8
- 230000035558 fertility Effects 0.000 abstract description 7
- 230000006870 function Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 10
- 241000208293 Capsicum Species 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 9
- 241000607479 Yersinia pestis Species 0.000 description 9
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 239000003513 alkali Substances 0.000 description 6
- 229940088598 enzyme Drugs 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 238000009331 sowing Methods 0.000 description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 5
- 229930003268 Vitamin C Natural products 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 235000019154 vitamin C Nutrition 0.000 description 5
- 239000011718 vitamin C Substances 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000005273 aeration Methods 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 235000021049 nutrient content Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 230000007269 microbial metabolism Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- WZLMXYBCAZZIRQ-UHFFFAOYSA-N [N].[P].[K] Chemical compound [N].[P].[K] WZLMXYBCAZZIRQ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079919 digestives enzyme preparation Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- -1 salt ions Chemical class 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/24—Lactobacillus brevis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/265—Micrococcus
- C12R2001/28—Micrococcus glutamicus ; Corynebacterium glutamicum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Pest Control & Pesticides (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Medicinal Chemistry (AREA)
- Inorganic Chemistry (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Plant Pathology (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of soil improvement materials, in particular to a soil conditioner for promoting the growth of capsicum and a preparation method thereof, wherein the soil conditioner mainly comprises the following raw materials in parts by weight: 20-35 parts of microbial preparation, 80-100 parts of corn stalk powder, 10-15 parts of water storage particles, 4-8 parts of flavin enzyme preparation, 5-10 parts of adhesive, 2-5 parts of trehalose and 15-20 parts of nutritional agent. The invention also provides a preparation method of the soil conditioner, the prepared soil conditioner can effectively reduce the salt alkalinity of soil, improve the soil fertility, simultaneously has the functions of storing water and improving the air permeability of the soil, provides a proper soil environment for the growth of crops, promotes the growth of crops and improves the yield.
Description
Technical Field
The invention relates to the technical field of soil improvement materials, in particular to a soil conditioner for promoting pepper growth and a preparation method thereof.
Background
The soil condition of pepper cultivation is an important factor for influencing pepper growth and determining pepper yield, common soil problems include insufficient fertility, insufficient nutrient supply, soil salinization, soil hardening, poor soil air permeability, soil diseases and insect pests and the like, and the factors can influence pepper growth to reduce the yield. The soil conditioner has the functions of improving soil, increasing crop yield and the like, and is a common soil improvement material in agriculture. Currently, there are two main types of soil conditioners commonly used in the market: one is to repair soil by using chemical substances, and to reduce the saline-alkali components in the soil by using the reaction of chemical reagents and the chemical substances in the soil; the other is to use microorganism to repair, which mainly uses a large amount of organic acid generated in the physiological activity of microorganism, can reduce the pH value of saline-alkali soil, and can change the saline-alkali components in soil, thereby reducing the saline-alkali amount. However, the chemical soil conditioner has quick response, good short-term effect, high cost, difficult removal of the chemical reagent after entering the soil, possibility of secondary pollution and high potential risk; the microbial conditioner has long action time, slow effect, poor short-term economic benefit and difficult popularization. In addition, the common soil conditioner is mainly used for salinization of soil, and has little research on soil problems such as soil diseases and insect pests, poor soil air permeability and the like, so that how to develop an efficient and environment-friendly multifunctional soil conditioner is a technical problem to be solved.
Disclosure of Invention
Aiming at the technical problems, the invention provides a soil conditioner for promoting the growth of capsicum and a preparation method thereof, wherein the soil conditioner comprises water storage particles, which can absorb redundant moisture in soil and store the moisture, and the water storage particles contain a large number of micropore structures, so that the hardening of the soil can be improved, and the air permeability of the soil can be enhanced; the microbial preparation is added, and a plurality of microorganisms are synergistic, so that the soil fertility can be obviously improved, the soil salt alkalinity can be reduced, and the effects of resisting diseases and insect pests can be achieved; the flavin enzyme preparation can be synergistic with a microbial preparation to further improve the soil improvement effect of the soil conditioner, improve the efficiency, promote the growth of capsicum and improve the yield.
The invention is realized by the following technical scheme:
the soil conditioner for promoting the growth of the peppers comprises the following raw materials in parts by weight: 20-35 parts of microbial preparation, 80-100 parts of corn stalk powder, 10-15 parts of water storage particles, 4-8 parts of flavin enzyme preparation, 5-10 parts of adhesive, 2-5 parts of trehalose and 15-20 parts of nutritional agent.
Further, the microbial preparation includes Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strains.
Further, the corn stalk powder is 40-50 meshes.
Further, the adhesive is one or more of bentonite, polyvinyl alcohol and alpha starch.
Further, the nutrient is prepared by mixing yeast extract, peptone and amino acid according to a mass ratio of 1:2:1.
The invention also provides a preparation method of the soil conditioner for promoting the growth of the capsicum, which comprises the following steps:
s1: uniformly mixing a microbial preparation, corn stalk powder, a flavin enzyme preparation, a 3/5 binding agent, trehalose and a nutritional agent, feeding into a disc granulator, granulating into balls, drying at 30 ℃ to 3-4 h, cooling to room temperature, and screening to obtain active particles with the particle size of 2-4 mm;
s2: and (2) adding the water storage particles into a spray nozzle hopper, adding the active particles prepared in the step (S1) into a disc flocking machine, coating the residual adhesive on the surfaces of the active particles under a rotating condition, starting a spray nozzle to spray the water storage particles on the surfaces of the active particles after coating, drying at 30 ℃ for 1-1.5 and h, cooling to room temperature, and screening to obtain the soil conditioner with the particle size of 6-8 mm for promoting the growth of the peppers.
Further, the preparation method of the water storage particles in the step S2 comprises the following steps:
(1) Ball milling a porous material, sieving with a 200-300 mesh sieve, mixing with aluminum chloride and magnesium sulfate according to a mass ratio of 7:1:1, placing in a conical flask, adding water, stirring to dissolve, adjusting pH to 7.2-7.8 with 1mol/L sodium hydroxide solution, oscillating by a shaker for 10-h, standing for 20-30 min, drying to constant weight, placing in a crucible, and calcining at 350-450 ℃ for 5-6 h to obtain a first modified material;
(2) Mixing the first modified material prepared in the step (1) with lauroyl chloride according to the mass ratio of 1:1, adding into a reaction kettle, adding deionized water with the mass ratio of 4:1 to the first modified material, stirring at 35 ℃ for 12-14 h, adding succinic anhydride with the mass ratio of 1:5 to the lauroyl chloride, and continuing stirring for 2-3 h to obtain a mixed solution;
(3) And (3) drying the mixed solution obtained in the step (2) at 60 ℃ for 14-16 h, washing with acetone, drying, ball-milling, and sieving with a 500-600 mesh sieve to obtain water storage particles.
Further, the porous material comprises one or more of zeolite and sepiolite.
Further, the preparation method of the microbial preparation in the step S1 comprises the following steps:
I. inoculating Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strain into LB liquid medium respectively, shake culturing at 30deg.C for 20-24 h to obtain inoculating solution;
II, inoculating the inoculating liquid obtained in the step I into an LB liquid culture medium according to the volume percentage of 2-5%, and shake culturing at 30 ℃ for 12-16 h to obtain seed liquid;
and III, inoculating the seed liquid obtained in the step II and a fermentation medium into a fermentation tank containing the fermentation medium according to the volume percentage of 1-2% for fermentation to obtain fermentation liquid, mixing the obtained fermentation liquid of lactobacillus brevis, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum according to the volume percentage of 4:3:1:2, centrifuging, freeze-drying the precipitate, and grinding the precipitate into powder to obtain the microbial preparation.
Further, the fermentation medium in the step III comprises 1% of sucrose, 0.02% of yeast extract, 0.05% of ammonium sulfate, 0.01% of magnesium sulfate, 0.01% of potassium chloride, 0.0001% of manganese sulfate, 0.01% of disodium hydrogen phosphate, 0.1% of calcium carbonate and the balance of water.
Further, the Lactobacillus brevis preservation unit is China general microbiological culture collection center, and the preservation number is CGMCC No.11758; the bifidobacterium longum preservation unit is China general microbiological culture collection center CGMCC No.23905; the preservation unit of the bacillus pantothenicum is China center for type culture collection (CCTCC AB 92072); the preservation unit of the corynebacterium glutamicum is China general microbiological culture collection center, the preservation number is CGMCC 1.15647, and the strains are disclosed strains.
Further, the fermentation condition of the fermentation tank in the step III is that the culture temperature is 28-32 ℃, the aeration rate is 1:1.5, the stirring speed is 300rpm, and the culture time is 30-35 h.
Compared with the prior art, the invention has the following beneficial effects:
the soil conditioner for promoting the growth of the capsicum prepared by the invention is added with the ingredients comprising short ingredientsMicrobial preparations of lactobacillus, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum strains, derivatives produced by microbial metabolism can improve the nutrient content of soil, enhance the fertility of soil, activate indissolvable elements of saline-alkali soil, improve the nutrient utilization rate, and neutralize OH by organic acid produced in the microbial metabolism process - Effectively reduces the salt alkalinity of soil. The invention utilizes the Lactobacillus brevis, can generate hydrogen peroxide under the conditions of flavinase and oxygen, has the effect of disease and insect resistance, and the riboflavin necessary for the flavinase can be generated by bifidobacterium longum, so that the activity of the flavinase can be improved, the efficiency of generating hydrogen peroxide by the Lactobacillus brevis can be further improved, the effect of disease and insect resistance is enhanced, and the amino acid generated in the metabolic processes of the Bacillus pantothenicum and the Corynebacterium glutamicum can provide nitrogen sources required by the growth of crops and other bacterial groups, promote the growth of the crops and the proliferation of bacterial groups, and promote the soil improvement effect. The zeolite and sepiolite are special in surface structure and are porous materials and have good adsorption and ion exchange properties, and the zeolite and sepiolite are modified to prepare water storage particles, so that the water storage particles are strong in adsorption, the water retention and fertilizer retention capacity of soil can be improved, salt ions can be adsorbed, salt damage can be reduced, the salinization of the soil is improved, the porous structure is beneficial to the formation of soil aggregate structures, the soil hardening is improved, the soil air permeability is enhanced, the improvement of the soil air permeability is more beneficial to the growth of crops, the biological activity of microorganisms can be improved, meanwhile, the water storage particles can adsorb and store excessive moisture in the soil, the damage to the crops caused by the too high water content of the soil is avoided, the water content of the soil can be kept, and the growth of the crops is facilitated. The water storage particles are coated on the surfaces of the active particles comprising microbial preparations through a flocking machine, and the prepared soil conditioner has the effects of effectively improving soil salinization and soil hardening, enhancing soil fertility and air permeability, resisting diseases and insect pests and regulating soil moisture content through the synergistic effect of various microorganisms, enzyme preparations and the water storage particles.
Drawings
In order to more clearly illustrate the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only of the invention and that other drawings can be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a graph showing the effect of the soil conditioner for promoting pepper growth on fresh weight of peppers according to examples 1-3 of the present invention;
FIG. 2 is a graph showing the effect of the soil conditioner for promoting pepper growth on the vitamin C content of peppers according to examples 1-3 of the present invention;
FIG. 3 is a graph showing the water retention performance test of the soil conditioner for promoting pepper growth according to examples 1-3 of the present invention;
fig. 4 is a graph showing that the soil conditioner for promoting pepper growth according to examples 1-3 of the present invention improves soil permeability.
Detailed Description
The present invention will be further described in detail with reference to the following specific examples, but the present invention is not limited to the following examples, for the purpose of making the objects, technical solutions and advantages of the present invention more apparent.
The chemical reagents related to the invention are purchased through commercial channels, and the strains are all disclosed strains, except for no special description.
Example 1: the embodiment provides a preparation method of a soil conditioner for promoting pepper growth, which mainly comprises the following steps:
preparation of microbial preparation:
I. inoculating Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strains into LB liquid medium respectively, shake culturing at 30deg.C for 20 h to obtain inoculating solution;
II, inoculating the inoculating liquid obtained in the step I into an LB liquid culture medium according to the volume percentage of 2%, and shake culturing at 30 ℃ for 12 h to obtain seed liquid;
III, inoculating the seed liquid obtained in the step II and a fermentation medium into a fermentation tank containing the fermentation medium according to the volume percentage of 1% for fermentation, wherein the fermentation condition of the fermentation tank is that the culture temperature is 28 ℃, the aeration rate is 1:1.5, the stirring speed is 300rpm, the culture time is 30 h, so as to obtain fermentation liquid, mixing the obtained fermentation liquid of lactobacillus brevis, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum according to the volume ratio of 4:3:1:2, centrifuging, freeze-drying the precipitate, and grinding the precipitate into powder to obtain a microbial preparation;
the fermentation medium in the step III comprises 1% of sucrose, 0.02% of yeast extract, 0.05% of ammonium sulfate, 0.01% of magnesium sulfate, 0.01% of potassium chloride, 0.0001% of manganese sulfate, 0.01% of disodium hydrogen phosphate, 0.1% of calcium carbonate and the balance of water.
Preparation of water storage particles:
(1) Ball milling zeolite, sieving with a 200-mesh sieve, mixing with aluminum chloride and magnesium sulfate according to the mass ratio of 7:1:1, placing in a conical flask, adding water, stirring to dissolve, adjusting the pH to 7.2 with 1mol/L sodium hydroxide solution, oscillating by a shaking table for 10 h, standing for 20 min, drying to constant weight, placing in a crucible, and calcining at 350 ℃ for 5 h to obtain a first modified material;
(2) Mixing the first modified material prepared in the step (1) with lauroyl chloride according to the mass ratio of 1:1, adding into a reaction kettle, adding deionized water with the mass ratio of 4:1 to the first modified material, stirring at 35 ℃ for 12 h, adding succinic anhydride with the mass ratio of 1:5 to the lauroyl chloride, and continuing stirring for 2 h to obtain a mixed solution;
(3) And (3) drying the mixed solution obtained in the step (2) at 60 ℃ for 14 h, washing with acetone, drying, ball-milling, and sieving with a 500-mesh sieve to obtain water storage particles.
Preparation of a soil conditioner for promoting pepper growth:
s1: uniformly mixing 20 g microbial preparation, 80 g corn stalk powder, 4 g flavin enzyme preparation, 3 g bentonite, 2 g trehalose and 15 g nutrient, feeding into a disc granulator, granulating to obtain balls, drying at 30 ℃ to 3 h, cooling to room temperature, and sieving to obtain active particles with particle size of 2 mm;
s2: adding 10 g water storage particles into a spray nozzle hopper, adding the active particles prepared in the step S1 into a disc flocking machine, coating 2 g bentonite on the surfaces of the active particles under a rotating condition, starting a spray nozzle to spray the water storage particles on the surfaces of the active particles after coating, drying at 30 ℃ for 1 h, cooling to room temperature, and screening to obtain the soil conditioner with the particle size of 6 mm for promoting the growth of capsicum;
the corn stalk powder in the step S1 of the embodiment is 40 meshes, and the nutrient is prepared by mixing yeast extract, peptone and amino acid according to the mass ratio of 1:2:1.
Example 2: the embodiment provides a preparation method of a soil conditioner for promoting pepper growth, which mainly comprises the following steps:
preparation of microbial preparation:
I. inoculating Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strains into LB liquid medium respectively, shake culturing at 30deg.C for 24 h to obtain inoculating solution;
II, inoculating the inoculating liquid obtained in the step I into an LB liquid culture medium according to the proportion of 5% by volume, and shake culturing at 30 ℃ for 16 h to obtain seed liquid;
III, inoculating the seed liquid obtained in the step II and a fermentation medium into a fermentation tank containing the fermentation medium according to the volume percentage of 2% for fermentation, wherein the fermentation condition of the fermentation tank is that the culture temperature is 32 ℃, the aeration rate is 1:1.5, the stirring speed is 300rpm, the culture time is 35 h, so as to obtain fermentation liquid, mixing the obtained fermentation liquid of lactobacillus brevis, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum according to the volume ratio of 4:3:1:2, centrifuging, freeze-drying the precipitate, and grinding the precipitate into powder to obtain a microbial preparation;
the fermentation medium in the step III comprises 1% of sucrose, 0.02% of yeast extract, 0.05% of ammonium sulfate, 0.01% of magnesium sulfate, 0.01% of potassium chloride, 0.0001% of manganese sulfate, 0.01% of disodium hydrogen phosphate, 0.1% of calcium carbonate and the balance of water.
Preparation of water storage particles:
(1) Ball milling sepiolite, sieving with a 200-300 mesh sieve, mixing with aluminum chloride and magnesium sulfate according to a mass ratio of 7:1:1, placing in a conical flask, adding water, stirring to dissolve, adjusting pH to 7.8 with 1mol/L sodium hydroxide solution, shaking 10 h by a shaking table, standing for 30 min, drying to constant weight, placing in a crucible, calcining at 450 ℃ for 6 h, and obtaining a first modified material;
(2) Mixing the first modified material prepared in the step (1) with lauroyl chloride according to the mass ratio of 1:1, adding into a reaction kettle, adding deionized water with the mass ratio of 4:1 to the first modified material, stirring at 35 ℃ for 14 h, adding succinic anhydride with the mass ratio of 1:5 to the lauroyl chloride, and continuing stirring for 3: 3 h to obtain a mixed solution;
(3) And (3) drying the mixed solution obtained in the step (2) at 60 ℃ for 16 h, washing with acetone, drying, ball-milling, and sieving with a 600-mesh sieve to obtain water storage particles.
Preparation of a soil conditioner for promoting pepper growth:
s1: uniformly mixing 35 g microbial preparation, 100 g corn stalk powder, 8 g flavin enzyme preparation, 6 g polyvinyl alcohol, 5 g trehalose and 20 g nutrient, feeding into a disc granulator, granulating to obtain balls, drying at 30 ℃ to 4 h, cooling to room temperature, and sieving to obtain active particles with particle diameter of 4 mm;
s2: adding 15 g water storage particles into a spray nozzle hopper, adding the active particles prepared in the step S1 into a disc flocking machine, coating 4 g polyvinyl alcohol on the surfaces of the active particles under a rotating condition, starting a spray nozzle to spray the water storage particles on the surfaces of the active particles after coating, drying at 30 ℃ for 1.5 h, cooling to room temperature, and screening to obtain the soil conditioner with the particle size of 8 mm for promoting the growth of capsicum;
in the embodiment, the corn stalk powder in the step S1 is 50 meshes, and the nutrient is prepared by mixing yeast extract, peptone and amino acid according to the mass ratio of 1:2:1.
Example 3: the embodiment provides a preparation method of a soil conditioner for promoting pepper growth, which mainly comprises the following steps:
preparation of microbial preparation:
I. inoculating Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strains into LB liquid medium respectively, shake culturing at 30deg.C for 22 h to obtain inoculating solution;
II, inoculating the inoculating liquid obtained in the step I into an LB liquid culture medium according to the proportion of 3% by volume, and shake culturing at 30 ℃ for 14 h to obtain seed liquid;
III, inoculating the seed liquid obtained in the step II and a fermentation medium into a fermentation tank containing the fermentation medium according to the volume percentage of 1.5%, fermenting under the fermentation conditions of a culture temperature of 30 ℃, an aeration rate of 1:1.5 and a stirring speed of 300rpm for 32-h to obtain a fermentation liquid, mixing the obtained fermentation liquid of lactobacillus brevis, bifidobacterium longum, bacillus pantothenicum and corynebacterium glutamicum according to the volume ratio of 4:3:1:2, centrifuging, freeze-drying the precipitate, and grinding the precipitate into powder to obtain a microbial preparation;
the fermentation medium in the step III comprises 1% of sucrose, 0.02% of yeast extract, 0.05% of ammonium sulfate, 0.01% of magnesium sulfate, 0.01% of potassium chloride, 0.0001% of manganese sulfate, 0.01% of disodium hydrogen phosphate, 0.1% of calcium carbonate and the balance of water.
Preparation of water storage particles:
(1) Mixing zeolite and sepiolite according to the mass ratio of 1:1, ball milling, sieving with a 250 mesh sieve, mixing with aluminum chloride and magnesium sulfate according to the mass ratio of 7:1:1, placing in a conical flask, adding water, stirring to dissolve, adjusting pH to 7.5 with 1mol/L sodium hydroxide solution, oscillating by a shaker for 10 h, standing for 25 min, drying to constant weight, placing in a crucible, calcining at 400 ℃ for 5.5 h, and obtaining a first modified material;
(2) Mixing the first modified material prepared in the step (1) with lauroyl chloride according to the mass ratio of 1:1, adding into a reaction kettle, adding deionized water with the mass ratio of 4:1 to the first modified material, stirring at 35 ℃ to 13 h, adding succinic anhydride with the mass ratio of 1:5 to the lauroyl chloride, and continuing stirring for 2.5 h to obtain a mixed solution;
(3) And (3) drying the mixed solution obtained in the step (2) at 60 ℃ for 15 h, washing with acetone, drying, ball-milling, and sieving with a 550-mesh sieve to obtain water storage particles.
Preparation of a soil conditioner for promoting pepper growth:
s1: uniformly mixing 30 g microbial preparation, 90 g corn stalk powder, 6 g flavin enzyme preparation, 4.8 g alpha starch, 3 g trehalose and 18 g nutrient, feeding into a disc granulator, granulating into balls, drying at 30 ℃ to 3.5 h, cooling to room temperature, and screening to obtain active particles with the particle size of 3 mm;
s2: adding 12 g water storage particles into a spray nozzle hopper, adding the active particles prepared in the step S1 into a disc flocking machine, coating 3.2 g alpha starch on the surfaces of the active particles under a rotating condition, starting a spray nozzle to spray the water storage particles on the surfaces of the active particles after coating, drying at 30 ℃ for 1.2 h, cooling to room temperature, and screening to obtain a soil conditioner with the particle size of 7 mm for promoting pepper growth;
in the embodiment, the corn stalk powder in the step S1 is 45 meshes, and the nutrient is prepared by mixing yeast extract, peptone and amino acid according to the mass ratio of 1:2:1.
Experimental example 1: the soil conditioner for promoting the growth of the peppers is adopted to carry out improvement test on saline-alkali barren lands in Ganzhou city, a land with relatively uniform soil overall condition is selected, the soil conditioner of the embodiment 1-3 is uniformly spread on the soil surface layer before sowing for two months, soil is ploughed for 20-cm, the soil conditioner is uniformly mixed with the soil, the consumption per mu is 100 kg, meanwhile, a CK group adopting a large water flood irrigation mode and a control group which is not treated are arranged, nutrient detection is carried out on soil samples taken two days before sowing, the sowing crop is peppers, the seedling emergence rate is measured two weeks after sowing, the result is shown in the table 1, the result shows that part of salt can be washed off by the large water flood irrigation mode (CK group), the pH value of the soil is reduced, a certain relieving effect on the alkalinity of the soil salt is achieved, and the seedling emergence rate is only 51.03%. After application of the soil conditioner of examples 1-3, the pH and salt content were both significantly reduced, with a maximum of 1.91 and 1.32 pH and a maximum of 69.4% and 54.2% salt content, respectively, compared to the control and CK groups. The emergence effect is remarkable, the emergence rate of the groups of examples 1-3 can reach more than 95%, and the CK group is only about 51.03%. The soil conditioner for promoting the growth of the peppers can effectively improve the salinization of soil and improve the emergence rate of the peppers.
TABLE 1 improvement of soil salinization by soil conditioner
Experimental example 2: 4 potting vessels of 150mm by 125mm (upper caliber by height) were used, each pot containing 1 kg air-dried soil, and the soil conditioner of examples 1 to 3 was applied at a rate of 0.8 g/kg, respectively. Uniformly mixing the soil conditioner with soil, filling into a basin, and watering thoroughly before sowing. The pepper seeds with the sprouted and white color are sown into the pots, and 6 pepper seeds are sown in each pot. The soil is kept moist before seeding until seedling emergence, water is poured once 2-3 days after seedling emergence, and the maximum field water holding capacity is kept at 40%. And (3) thinning out when 2-3 true leaves grow, keeping 2 plants in each pot at a distance of 3-4cm, and watering for 1 time after thinning out the seedlings. After 60 days, the soil was harvested, the stalks of 0.5. 0.5 cm were cut off for each pepper, and the organic matter, the effective nitrogen-phosphorus-potassium content, the biomass of the pepper and the Vitamin C (VC) content of each group of soil were measured, and the results are shown in table 2 and fig. 1-2. The results in Table 2 show that the soil conditioner of examples 1-3 of the present invention is applied to significantly increase the organic matter content of the soil and improve the soil fertility. Compared with the control group, the soil organic matters in the examples 1-3 are increased by 44.17% -51.44%. In addition, the application of the soil conditioner of examples 1-3 significantly increased the available nutrient content of the soil. The results of fig. 1 show that the fresh weight of the peppers in the embodiment 1-3 is more than 1.7 times that of the control group, and the results of fig. 2 show that the content of the vitamin C in the peppers in the embodiment 1-3 is obviously higher than that in the control group, and the content of the vitamin C in the peppers is improved by more than 40% compared with that in the control group, so that the soil conditioner can effectively promote the growth of the peppers.
Table 2 soil nutrient content
Experimental example 3: selecting adjacent lands with similar soil conditions, uniformly spreading the soil conditioner of the examples 1-3 on the soil surface layer, ploughing the soil by 20 cm to ensure that the conditioner is uniformly mixed with the soil, setting a control group without adding the soil conditioner and a CK group with applying equal amount of the Schdanli compound fertilizer, sowing peppers, counting the yield and the occurrence of diseases and insect pests of each group after 90 days, and the results are shown in the table 3, wherein the results in the table 3 show that the occurrence rate of the diseases and insect pests is obviously reduced compared with the control group and the CK group after applying the soil conditioner of the examples 1-3, and the yield of the peppers is higher than that of the control group and the CK group. Pest incidence (%) = number of pest-infected capsicum plants/total capsicum plants. The results in table 3 show that the soil conditioner for promoting the growth of the peppers can reduce the incidence rate of plant diseases and insect pests and improve the yield of the peppers.
TABLE 3 incidence of pests and Capsici fructus yield
Experimental example 4: the soil conditioner of examples 1-3 was taken 5 g each, mixed with 1 kg of soil from the same block, and then added with 20% of water by weight of the soil, thoroughly stirred, while a control group to which no soil conditioner was added and a CK group to which an equal amount of a smith danish compound fertilizer was applied were simultaneously set, each group of samples was weighed, the initial weights were recorded, the soil moisture evaporation experiments were performed under the same conditions (constant temperature 20 ℃) and after 5, 15, 30, 45 and 60 days, each group was weighed, the current weights were recorded, and the moisture loss rate in the soil was calculated, moisture loss rate (%) = (initial weight-current weight)/initial weight, and the results are shown in fig. 3. The results of fig. 3 show that the bio-organic compound fertilizer has good water holding capacity, can delay the loss of soil moisture and can keep the soil moist for a long time.
Experimental example 5: the soil conditioner of examples 1 to 3 was taken to be mixed with 1 kg of the soil taken from the same block, each 5 g was stirred sufficiently, a control group to which no soil conditioner was added and a CK group to which an equal amount of the smith compound fertilizer was applied were simultaneously set, left to stand naturally for 12 months under the same indoor environment (constant temperature 20 ℃ with relative humidity 40% rh), then the volume weight of the soil was measured by a knife-over-loop method, and the porosity of the soil was calculated, and the results are shown in fig. 4. Porosity (%) = (1-volume weight/specific gravity) ×100%, specific gravity referring to the volume actually occupied by the solid particles of soil, typically 2.65 g/cm 3 To calculate. The results of fig. 4 show that the soil conditioner of examples 1-3 significantly reduces the volume weight of soil and increases the porosity of soil compared with the control group and the CK group, which indicates that the soil conditioner for promoting the growth of capsicum can improve the permeability of soil and enhance the air permeability of soil.
In conclusion, the soil conditioner for promoting the growth of the peppers can enhance the soil fertility, improve the salinization of the soil, enhance the air permeability of the soil, create a soil environment suitable for the growth of crops, promote the growth of the crops and improve the yield of the crops.
Those of ordinary skill in the art will appreciate that: the discussion of any of the embodiments above is merely exemplary and is not intended to suggest that the scope of the invention (including the claims) is limited to these examples; the technical features of the above embodiments or in the different embodiments may also be combined within the idea of the invention, the steps may be implemented in any order and there are many other variations of the different aspects of the invention as described above, which are not provided in detail for the sake of brevity.
The present invention is intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, improvement, etc. of the present invention should be included in the scope of the present invention.
Claims (10)
1. The soil conditioner for promoting the growth of the peppers is characterized by comprising the following raw materials in parts by weight: 20-35 parts of microbial preparation, 80-100 parts of corn stalk powder, 10-15 parts of water storage particles, 4-8 parts of flavin enzyme preparation, 5-10 parts of adhesive, 2-5 parts of trehalose and 15-20 parts of nutritional agent.
2. A soil conditioner for promoting pepper growth as claimed in claim 1, wherein said microbial preparation comprises lactobacillus brevis, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum strains.
3. A soil conditioner for promoting pepper growth as claimed in claim 2, wherein said corn stalk powder is 40-50 mesh.
4. A soil conditioner for promoting pepper growth as claimed in claim 3, wherein said binder is one or more of bentonite, polyvinyl alcohol, alpha starch.
5. The soil conditioner for promoting pepper growth as claimed in claim 4, wherein said nutrient is a mixture of yeast extract, peptone and amino acid in a mass ratio of 1:2:1.
6. A method for preparing a soil conditioner for promoting pepper growth as claimed in any one of the claims 1-5, comprising the steps of:
s1: uniformly mixing a microbial preparation, corn stalk powder, a flavin enzyme preparation, a 3/5 binding agent, trehalose and a nutritional agent, feeding into a disc granulator, granulating into balls, drying at 30 ℃ to 3-4 h, cooling to room temperature, and screening to obtain active particles with the particle size of 2-4 mm;
s2: and (2) adding the water storage particles into a spray nozzle hopper, adding the active particles prepared in the step (S1) into a disc flocking machine, coating the residual adhesive on the surfaces of the active particles under a rotating condition, starting a spray nozzle to spray the water storage particles on the surfaces of the active particles after coating, drying at 30 ℃ for 1-1.5 and h, cooling to room temperature, and screening to obtain the soil conditioner with the particle size of 6-8 mm for promoting the growth of the peppers.
7. The method for preparing a soil conditioner for promoting pepper growth as claimed in claim 6, wherein the method for preparing the microbial preparation in step S1 comprises the following steps:
I. inoculating Lactobacillus brevis, bifidobacterium longum, cladosporium pantothenicum and Corynebacterium glutamicum strain into LB liquid medium respectively, shake culturing at 30deg.C for 20-24 h to obtain inoculating solution;
II, inoculating the inoculating liquid obtained in the step I into an LB liquid culture medium according to the volume percentage of 2-5%, and shake culturing at 30 ℃ for 12-16 h to obtain seed liquid;
and III, inoculating the seed liquid obtained in the step II and a fermentation medium into a fermentation tank containing the fermentation medium according to the volume percentage of 1-2%, fermenting under the fermentation conditions of 28-32 ℃ of culture temperature, 1:1.5 of ventilation rate and 300rpm of stirring speed, and 30-35 h of culture time to obtain a fermentation liquid, mixing the obtained fermentation liquid of lactobacillus brevis, bifidobacterium longum, bacillus thuringiensis and corynebacterium glutamicum according to the volume ratio of 4:3:1:2, centrifuging, freeze-drying the precipitate, and grinding the precipitate into powder to obtain the microbial preparation.
8. The method for preparing the soil conditioner for promoting the growth of capsicum according to claim 7, wherein the fermentation medium in the step III comprises 1% of sucrose, 0.02% of yeast extract, 0.05% of ammonium sulfate, 0.01% of magnesium sulfate, 0.01% of potassium chloride, 0.0001% of manganese sulfate, 0.01% of disodium hydrogen phosphate, 0.1% of calcium carbonate and the balance of water.
9. The method for preparing soil conditioner for promoting pepper growth as claimed in claim 8, wherein the preparing method of the water storage granule in step S2 comprises the following steps:
(1) Ball milling a porous material, sieving with a 200-300 mesh sieve, mixing with aluminum chloride and magnesium sulfate according to a mass ratio of 7:1:1, placing in a conical flask, adding water, stirring to dissolve, adjusting pH to 7.2-7.8 with 1mol/L sodium hydroxide solution, oscillating by a shaker for 10-h, standing for 20-30 min, drying to constant weight, placing in a crucible, and calcining at 350-450 ℃ for 5-6 h to obtain a first modified material;
(2) Mixing the first modified material prepared in the step (1) with lauroyl chloride according to the mass ratio of 1:1, adding into a reaction kettle, adding deionized water with the mass ratio of 4:1 to the first modified material, stirring at 35 ℃ for 12-14 h, adding succinic anhydride with the mass ratio of 1:5 to the lauroyl chloride, and continuing stirring for 2-3 h to obtain a mixed solution;
(3) And (3) drying the mixed solution obtained in the step (2) at 60 ℃ for 14-16 h, washing with acetone, drying, ball-milling, and sieving with a 500-600 mesh sieve to obtain water storage particles.
10. The method for preparing a soil conditioner for promoting pepper growth as recited in claim 9, wherein said porous material of step (1) comprises one or more of zeolite and sepiolite.
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| CN114525218A (en) * | 2021-12-30 | 2022-05-24 | 美益添生物医药(武汉)有限公司 | Bifidobacterium longum, culture method thereof and application thereof in high yield of gamma-aminobutyric acid and 5-hydroxytryptamine |
| CN114525218B (en) * | 2021-12-30 | 2024-05-07 | 美益添生物医药(武汉)有限公司 | Bifidobacterium longum, culture method thereof and application of bifidobacterium longum in high-yield gamma-aminobutyric acid and 5-hydroxytryptamine |
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