CN117500917A - 融合蛋白、融合蛋白的制造方法、电极、氧化还原装置、氧化还原方法、二硫键的切断方法和过敏原的灭活方法 - Google Patents

融合蛋白、融合蛋白的制造方法、电极、氧化还原装置、氧化还原方法、二硫键的切断方法和过敏原的灭活方法 Download PDF

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Publication number
CN117500917A
CN117500917A CN202280043276.0A CN202280043276A CN117500917A CN 117500917 A CN117500917 A CN 117500917A CN 202280043276 A CN202280043276 A CN 202280043276A CN 117500917 A CN117500917 A CN 117500917A
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China
Prior art keywords
fusion protein
electrode
linker peptide
redox
thioredoxin
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CN202280043276.0A
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English (en)
Chinese (zh)
Inventor
初谷纪幸
和山文哉
奥村泰章
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Panasonic Intellectual Property Management Co Ltd
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Panasonic Intellectual Property Management Co Ltd
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Publication of CN117500917A publication Critical patent/CN117500917A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01009—Thioredoxin-disulfide reductase (1.8.1.9), i.e. thioredoxin-reductase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/07—Oxidoreductases acting on sulfur groups as donors (1.8) with an iron-sulfur protein as acceptor (1.8.7)
    • C12Y108/07002—Ferredoxin:thioredoxin reductase (1.8.7.2)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
CN202280043276.0A 2021-06-23 2022-06-23 融合蛋白、融合蛋白的制造方法、电极、氧化还原装置、氧化还原方法、二硫键的切断方法和过敏原的灭活方法 Pending CN117500917A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-104343 2021-06-23
JP2021104343 2021-06-23
PCT/JP2022/025134 WO2022270588A1 (ja) 2021-06-23 2022-06-23 融合タンパク質、融合タンパク質の製造方法、電極、酸化還元装置、酸化還元方法、ジスルフィド結合の切断方法、及び、アレルゲンの不活化方法

Publications (1)

Publication Number Publication Date
CN117500917A true CN117500917A (zh) 2024-02-02

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Family Applications (1)

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CN202280043276.0A Pending CN117500917A (zh) 2021-06-23 2022-06-23 融合蛋白、融合蛋白的制造方法、电极、氧化还原装置、氧化还原方法、二硫键的切断方法和过敏原的灭活方法

Country Status (7)

Country Link
US (1) US20240117325A1 (https=)
EP (1) EP4361179A4 (https=)
JP (1) JPWO2022270588A1 (https=)
CN (1) CN117500917A (https=)
AU (1) AU2022296206A1 (https=)
CA (1) CA3222715A1 (https=)
WO (1) WO2022270588A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4563699A4 (en) * 2022-07-26 2025-11-26 Panasonic Ip Man Co Ltd ENZYMATIC ELECTRODE, ENZYMATIC REACTION DEVICE, ENZYMATIC REACTION METHOD, MODIFIED PROTEIN AND METHOD FOR PRODUCING MODIFIED PROTEIN
CN121057808A (zh) * 2023-05-16 2025-12-02 松下知识产权经营株式会社 酶反应装置和酶反应方法
CN118475621A (zh) 2023-06-19 2024-08-09 杭州恩和生物科技有限公司 生产7-脱氢胆固醇的融合多肽及其使用方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4570327B2 (ja) * 2000-12-19 2010-10-27 セムバイオシス ジェネティクス インコーポレイテッド 多量体タンパク質の生産のための方法、および関連組成物
JP2005505250A (ja) * 2001-05-04 2005-02-24 ゼンコー 核酸およびチオレドキシンレダクターゼ活性を有するタンパク質
US20030211511A1 (en) * 2001-05-04 2003-11-13 Briggs Steven P. Nucleic acids and proteins with thioredoxin reductase activity
JP5247043B2 (ja) * 2006-02-10 2013-07-24 キヤノン株式会社 試料中のチオレドキシン類の濃度に関する情報取得装置、ストレス度情報取得装置及びストレス度判定方法
JP2009002689A (ja) * 2007-06-19 2009-01-08 Canon Inc チオレドキシン類測定用電極、チオレドキシン類の測定方法、チオレドキシン類の測定装置
CN101899460B (zh) * 2010-02-24 2013-06-19 浙江大学 一种制备降低食物过敏原反应的融合蛋白的方法
CN115768865A (zh) * 2020-06-23 2023-03-07 松下知识产权经营株式会社 过敏原的灭活方法及过敏原灭活装置
CA3188175A1 (en) * 2020-06-23 2021-12-30 Panasonic Intellectual Property Management Co., Ltd. Method for cleaving disulfide bond in protein and device for cleaving disulfide bond in protein

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Publication number Publication date
EP4361179A4 (en) 2024-12-25
EP4361179A1 (en) 2024-05-01
JPWO2022270588A1 (https=) 2022-12-29
US20240117325A1 (en) 2024-04-11
AU2022296206A1 (en) 2024-01-04
WO2022270588A1 (ja) 2022-12-29
CA3222715A1 (en) 2022-12-29

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