CA3222715A1 - Fusion protein, fusion protein production method, electrode, redox device, redox method, method for cutting disulfide bonds, and method for inactivating allergen - Google Patents

Fusion protein, fusion protein production method, electrode, redox device, redox method, method for cutting disulfide bonds, and method for inactivating allergen Download PDF

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Publication number
CA3222715A1
CA3222715A1 CA3222715A CA3222715A CA3222715A1 CA 3222715 A1 CA3222715 A1 CA 3222715A1 CA 3222715 A CA3222715 A CA 3222715A CA 3222715 A CA3222715 A CA 3222715A CA 3222715 A1 CA3222715 A1 CA 3222715A1
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CA
Canada
Prior art keywords
electrode
fusion protein
linker peptide
linker
redox
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3222715A
Other languages
English (en)
French (fr)
Inventor
Noriyuki HATSUGAI
Fumiya WAYAMA
Yasuaki Okumura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panasonic Intellectual Property Management Co Ltd
Original Assignee
Panasonic Intellectual Property Management Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Panasonic Intellectual Property Management Co Ltd filed Critical Panasonic Intellectual Property Management Co Ltd
Publication of CA3222715A1 publication Critical patent/CA3222715A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/01—Oxidoreductases acting on sulfur groups as donors (1.8) with NAD+ or NADP+ as acceptor (1.8.1)
    • C12Y108/01009—Thioredoxin-disulfide reductase (1.8.1.9), i.e. thioredoxin-reductase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/07—Oxidoreductases acting on sulfur groups as donors (1.8) with an iron-sulfur protein as acceptor (1.8.7)
    • C12Y108/07002—Ferredoxin:thioredoxin reductase (1.8.7.2)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
CA3222715A 2021-06-23 2022-06-23 Fusion protein, fusion protein production method, electrode, redox device, redox method, method for cutting disulfide bonds, and method for inactivating allergen Pending CA3222715A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-104343 2021-06-23
JP2021104343 2021-06-23
PCT/JP2022/025134 WO2022270588A1 (ja) 2021-06-23 2022-06-23 融合タンパク質、融合タンパク質の製造方法、電極、酸化還元装置、酸化還元方法、ジスルフィド結合の切断方法、及び、アレルゲンの不活化方法

Publications (1)

Publication Number Publication Date
CA3222715A1 true CA3222715A1 (en) 2022-12-29

Family

ID=84544446

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3222715A Pending CA3222715A1 (en) 2021-06-23 2022-06-23 Fusion protein, fusion protein production method, electrode, redox device, redox method, method for cutting disulfide bonds, and method for inactivating allergen

Country Status (7)

Country Link
US (1) US20240117325A1 (https=)
EP (1) EP4361179A4 (https=)
JP (1) JPWO2022270588A1 (https=)
CN (1) CN117500917A (https=)
AU (1) AU2022296206A1 (https=)
CA (1) CA3222715A1 (https=)
WO (1) WO2022270588A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4563699A4 (en) * 2022-07-26 2025-11-26 Panasonic Ip Man Co Ltd ENZYMATIC ELECTRODE, ENZYMATIC REACTION DEVICE, ENZYMATIC REACTION METHOD, MODIFIED PROTEIN AND METHOD FOR PRODUCING MODIFIED PROTEIN
CN121057808A (zh) * 2023-05-16 2025-12-02 松下知识产权经营株式会社 酶反应装置和酶反应方法
CN118475621A (zh) 2023-06-19 2024-08-09 杭州恩和生物科技有限公司 生产7-脱氢胆固醇的融合多肽及其使用方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4570327B2 (ja) * 2000-12-19 2010-10-27 セムバイオシス ジェネティクス インコーポレイテッド 多量体タンパク質の生産のための方法、および関連組成物
JP2005505250A (ja) * 2001-05-04 2005-02-24 ゼンコー 核酸およびチオレドキシンレダクターゼ活性を有するタンパク質
US20030211511A1 (en) * 2001-05-04 2003-11-13 Briggs Steven P. Nucleic acids and proteins with thioredoxin reductase activity
JP5247043B2 (ja) * 2006-02-10 2013-07-24 キヤノン株式会社 試料中のチオレドキシン類の濃度に関する情報取得装置、ストレス度情報取得装置及びストレス度判定方法
JP2009002689A (ja) * 2007-06-19 2009-01-08 Canon Inc チオレドキシン類測定用電極、チオレドキシン類の測定方法、チオレドキシン類の測定装置
CN101899460B (zh) * 2010-02-24 2013-06-19 浙江大学 一种制备降低食物过敏原反应的融合蛋白的方法
CN115768865A (zh) * 2020-06-23 2023-03-07 松下知识产权经营株式会社 过敏原的灭活方法及过敏原灭活装置
CA3188175A1 (en) * 2020-06-23 2021-12-30 Panasonic Intellectual Property Management Co., Ltd. Method for cleaving disulfide bond in protein and device for cleaving disulfide bond in protein

Also Published As

Publication number Publication date
EP4361179A4 (en) 2024-12-25
EP4361179A1 (en) 2024-05-01
JPWO2022270588A1 (https=) 2022-12-29
US20240117325A1 (en) 2024-04-11
AU2022296206A1 (en) 2024-01-04
WO2022270588A1 (ja) 2022-12-29
CN117500917A (zh) 2024-02-02

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