CN1174659A - Synergistic vcalidamycin A composition - Google Patents
Synergistic vcalidamycin A composition Download PDFInfo
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- CN1174659A CN1174659A CN 97106542 CN97106542A CN1174659A CN 1174659 A CN1174659 A CN 1174659A CN 97106542 CN97106542 CN 97106542 CN 97106542 A CN97106542 A CN 97106542A CN 1174659 A CN1174659 A CN 1174659A
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- synergistic
- vcalidamycin
- composition
- jinggangmycin
- aqua
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Abstract
The present invention discloses a kind of synergistic validamycin A composition for preventing and curing banded sclerotial blight of wheat and rice false smut of rice plant. It consists of effective component validamycin A and synergistic bacterial strain SD23. As compared with the single validamycin A the invented composition can raise by 11.1-20% prevention and cure results and inoreased output by 6-10%. Said invented is safe and high in efficiency, can be substituted for agricultural chemicals or can use a small quantity of agricultural chemicals, and possesses important significance for developing green food and nuisanceless agriculture.
Description
The present invention relates to a kind of biologic product of preventing and treating fungal diseases such as rice sheath blight disease, wheat sharp eyespot and rice green smut, specifically is a kind of Synergistic vcalidamycin A composition.
Prevent and treat the biological antibiotic element of rice sheath blight disease---jinggangmycin be leveraged to modern China existing two more than ten years history, because the unicity of preparation, drug effect is descended to some extent, even the escalated dose effect is still undesirable, how to improve jinggangmycin the new preparation of control efficiency, the developmental research of rice sheath blight disease is made it to surpass the original control efficiency of jinggangmycin, become the focus of those skilled in the art's tackling key problem.
The inventor is through research for many years, by adding a kind of new bacterial strain SD with synergistic effect that cultivates from DM 423 bacterial strains
23Form the synergy Synergistic combinations with jinggangmycin, thereby solved this difficult problem.Described new bacterial strain SD
23Be cured shape bacillus (Bacillus Cereus) that its preserving number is CGMCC NO.310.Its mycology property is as follows:
Qualification result: synergic bacteria SD
23(Bacillus Cereus) the object of the present invention is to provide a kind of Synergistic vcalidamycin A composition for cured shape bacillus.
| Colonial morphology | Type | Round | |
| The surface | Become the Chinese wax shape, low protruding | ||
| The edge | More neat, wavy | ||
| Color | Milky white | ||
| Optical signature | Opaque | ||
| Individual morphology | Gram | Positive | |
| Shape | Shaft-like | ||
| Arrange | Single, two or chaining | ||
| Size | ??1.0-1.2×20-50μm | ||
| Gemma | Shape | Ovum circle-cylindrical | |
| The position | Middle giving birth to-inferior end is given birth to | ||
| Expand | ????- | ||
| Poly- | ????+ | ||
| Parasporal crystal | ????- | ||
| Motility | It is very slow to move | ||
| Anaerobic growth | ????+ | ||
| Catalase | ????+ | ||
| ????5％??NaCl | ????+ | ||
| ????7％??NaCl | ????+ | ||
| ???pH5.7 | Oblique | ????+ | |
| Liquid | ????+ | ||
| Growth temperature ℃ | ??50℃ | ????- | |
| ??48℃ | ????- | ||
| ??45℃ | ????+ | ||
| ??15℃ | ????+ | ||
| ??10℃ | ????- | ||
| Litmus milk | Peptonize | ||
| The casein hydrolysis | ????+ | ||
| The starch hydrolysis | ????+ | ||
| M.R. test | ????+ | ||
| V.P. reaction | ????+ | ||
| Nitrate reduction | ????+ | ||
| Citrate utilizes | ????+ | ||
| The sugar alcohol fermentation and acid | Arabinose | ????- | |
| Wood sugar | ????- | ||
| Mannitol | ????- | ||
| Starch | ????+ | ||
| Maltose | ????+ | ||
| Sucrose | ????+ | ||
| Lactose | ????- | ||
| Glucose | ????+ | ||
| From the glucose aerogenesis | ????- | ||
Above-mentioned purpose of the present invention realizes in the following manner:
A kind of Synergistic vcalidamycin A wetting powder composition is characterized in that comprising containing jinggangmycin 1.0 * 10 in every gram
4～50 * 10
4μ g/g and synergic bacteria SD
231.0 * 10
4～90 * 10
4μ g/g, described synergic bacteria SD
23For cured shape bacillus (Bacillus Cereus) preserving number is CGMCC NO.310, and agricultural goes up acceptable assistant.
It is characterized in that described auxiliary agent is selected from dispersing agent MF, washing agent LS and/or penetrating agent BX.
It is characterized in that containing in every gram jinggangmycin 10 * 10
4μ g/g and synergic bacteria SD
2350 * 10
4μ g/g.
A kind of Synergistic vcalidamycin A aqua composition is characterized in that comprising containing jinggangmycin 1 * 10 in every milliliter of aqua
4μ g/g～20 * 10
4μ g/g and synergic bacteria SD
231.0 * 10
4μ g/g～90 * 10
4μ g/g, described synergic bacteria SD
23Be cured shape bacillus (Bacillus Cereus) that preserving number is CGMCC NO.310, and agricultural goes up acceptable misfortune agent.
It is characterized in that comprising and contain jinggangmycin 2.5 * 10 in every milliliter of aqua
4μ g/g and contain SD
23Synergic bacteria 50 * 10
4μ g/g.
Advantage of the present invention is significantly, can be from the different control efficiency of following digital proof to rice sheath blight disease:
Single with jinggangmycin control efficiency (%)
Every mu with 200ml 70.09
Every mu with 250ml 74.68
Contain jinggangmycin 2.5 * 10 and adopt Synergistic vcalidamycin A wetting powder of the present invention or aqua to be made into every milliliter
4μ g/g, contain synergic bacteria SD
2310 * 10
4μ g/g uses the 200ml aqua for every mu, the initial demonstration in Shanghai area, is tested to and applies 90～95 years accumulative total and use area and surpassed 2,000 ten thousand mu times.According to Shanghai City plant test station for plant protection evidence: to paddy and wheat sheath blight disease controlling effect is 84～93%; To the rice green smut control efficiency is 86～95%.According to the special test of Zhejiang Province's plant protection master station and Shanghai City plant protection master station, Synergistic vcalidamycin A and the contrast of single jinggangmycin increase by 11.1～20 percentage points, 6～10 percentage points of effect of increasing production raisings to the preventive effect of rice sheath blight disease.
Make because Synergistic vcalidamycin A system adopts biological disease-resistant type yield increasing fungus and biological antibiotic is compound, therefore have the double effects of antibiotic and disease-resistant bacterium concurrently.Improved preventive effect, increased output, made this old product of jinggangmycin shine the youth.For China's Rice Production provides safe, efficient, of new generation biopesticide.Applying biological active ingredient instead of chemical agricultural chemicals or use chemical pesticide less, to the development pollution-free food, pollution-less agriculture has the meaning of particular importance.
The present invention is described in further detail below with reference to specific embodiment, so that advantage of the present invention is more obvious.Embodiment 1～12: Synergistic vcalidamycin A aqua composition
The active principle content of batching: jinggangmycin 5.0 * 10
4μ g/g
Synergic bacteria SD
2320 * 10
4The above-mentioned synergic bacteria SD of μ g/g
23Be cured shape bacillus (Bacillus Cereus) that submit China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation by the applicant on June 25th, 1997, preserving number is CGMCC NO.0310.
By above-mentioned jinggangmycin and synergic bacteria SD
23With the combination of following ratio, and add agricultural and go up acceptable assistant and be selected from dispersing agent MF, washing agent LS and/or penetrating agent BX and an amount of water, form aqua composition with following effective component content:
Sequence number ratio jinggangmycin μ g/ml synergic bacteria SD
23μ g/ml
Embodiment 11: 1 2.5 * 10
410 * 10
4
Embodiment 21: 2 1.67 * 10
413.3 * 10
4
Embodiment 31: 3 1.25 * 10
415.0 * 10
4
Embodiment 41: 4 1.0 * 10
416.0 * 10
4
Embodiment 52: 1 3.33 * 10
46.7 * 10
4
Embodiment 62: 3 2.0 * 10
412.0 * 10
4
Embodiment 73: 1 3.75 * 10
45.0 * 10
4
Embodiment 83: 2 3.0 * 10
48.0 * 10
4
Embodiment 93: 4 2.14 * 10
411.4 * 10
4
Embodiment 10 4: 1 4.0 * 10
44.0 * 10
4
Embodiment 11 4: 3 2.86 * 10
48.6 * 10
4
Embodiment 12 5: 1 4.17 * 10
43.33 * 10
4Embodiment 13～24: Synergistic vcalidamycin A wetting powder composition
The active principle content of batching: jinggangmycin 100 * 10
4μ g/g
Synergic bacteria SD
23180 * 10
4μ g/g
Plain and the synergic bacteria SD with above-mentioned well hilllock
23With the combination of following ratio, and add agricultural and go up acceptable assistant and be selected from dispersing agent MF, washing agent LS and/or penetrating agent BX, form wetting powder composition with following effective component content:
Sequence number ratio jinggangmycin μ g/g synergic bacteria SD
23μ g/g
Embodiment 11: 1 50 * 10
490 * 10
4
Embodiment 21: 2 33.3 * 10
430 * 10
4
Embodiment 31: 3 25.0 * 10
422.5 * 10
4
Embodiment 41: 4 20.0 * 10
418.0 * 10
4
Embodiment 52: 1 66.6 * 10
460.0 * 10
4
Embodiment 62: 3 40.0 * 10
410.8 * 10
4
Embodiment 73: 1 75.0 * 10
445.0 * 10
4
Embodiment 83: 2 60.0 * 10
472.0 * 10
4
Embodiment 93: 4 42.8 * 10
4102.8 * 10
4
Embodiment 10 4: 1 80.0 * 10
430.0 * 10
4
Embodiment 11 4: 3 57.1 * 10
477.1 * 10
4
Embodiment 12 5: 1 83.3 * 10
430.0 * 10
4Application Example:
With 200 milliliters of every mu of consumptions of Synergistic vcalidamycin A aqua composition of embodiment 1～12, as follows to the control efficiency of rice sheath blight disease:
Embodiment sequence number control efficiency %
1??????????????92.29
2??????????????84.67
3??????????????82.54
4??????????????79.22
5??????????????81.46
6??????????????87.32
7??????????????78.54
8??????????????86.58
9??????????????90.14
10?????????????75.14
11?????????????89.56
12?????????????73.45
As adopt embodiment 13～24 Synergistic vcalidamycin A wetting powder compositions to be made into the aqua of the effective component content of embodiment 1～12, the control of rice sheath blight disease is had identical effect.
Simultaneously, Synergistic vcalidamycin A composition of the present invention has significant effect to the control of wheat banded sclerotial blight and the control of rice green smut.It is compared with single jinggangmycin, and the control efficiency of rice sheath blight disease has been improved 11.1～20%, and effect of increasing production improves 6～10%.
The foregoing description only for illustrative purposes, protection scope of the present invention will embody in claims.
Claims (6)
1. a Synergistic vcalidamycin A wetting powder composition is characterized in that comprising containing jinggangmycin 1.0 * 10 in every gram
4～50 * 10
4μ g/g and synergic bacteria SD
231.0 * 10
4～90 * 10
4μ g/g, described synergic bacteria SD
23For cured shape bacillus (Bacillus Cereus) preserving number is CGMCC NO.310, and agricultural goes up acceptable assistant.
2. according to the Synergistic vcalidamycin A wetting powder composition of claim 1, it is characterized in that described auxiliary agent is selected from dispersing agent MF, washing agent LS and/or penetrating agent BX.
3. according to the Synergistic vcalidamycin A wetting powder composition of claim 1 or 2, it is characterized in that containing in every gram jinggangmycin 10 * 10
4μ g/g and synergic bacteria SD
2350 * 10
4μ g/g.
4. a Synergistic vcalidamycin A aqua composition is characterized in that comprising containing jinggangmycin 1 * 10 in every milliliter of aqua
4μ g/g～20 * 10
4μ g/g and synergic bacteria 5D
231.0 * 10
4μ g/g～90 * 10
4μ g/g, described synergic bacteria SD
23Be cured shape bacillus (Bacillus Cereus) that preserving number is CGMCC NO.310, and agricultural goes up acceptable assistant.
5. aqua composition according to claim 4 is characterized in that described auxiliary agent is selected from dispersing agent MF, washing agent LS and/or penetrating agent BX.
6. according to claim 4 or 5 described aqua compositions, it is characterized in that comprising containing jinggangmycin 2.5 * 10 in every milliliter of aqua
4μ g/g and SD
23Synergic bacteria 50 * 10
4μ g/g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97106542 CN1116800C (en) | 1997-08-01 | 1997-08-01 | Synergistic vcalidamycin A composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97106542 CN1116800C (en) | 1997-08-01 | 1997-08-01 | Synergistic vcalidamycin A composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1174659A true CN1174659A (en) | 1998-03-04 |
| CN1116800C CN1116800C (en) | 2003-08-06 |
Family
ID=5168776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97106542 Expired - Lifetime CN1116800C (en) | 1997-08-01 | 1997-08-01 | Synergistic vcalidamycin A composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1116800C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392743A (en) * | 2013-08-13 | 2013-11-20 | 广东省农业科学院植物保护研究所 | Sterilization composition containing bacillus and echinomycin as well as preparation method and application thereof |
| CN107094797A (en) * | 2017-03-17 | 2017-08-29 | 南京工业大学 | Process for preparing biological pesticide by compounding bacillus cereus and antibiotics |
-
1997
- 1997-08-01 CN CN 97106542 patent/CN1116800C/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392743A (en) * | 2013-08-13 | 2013-11-20 | 广东省农业科学院植物保护研究所 | Sterilization composition containing bacillus and echinomycin as well as preparation method and application thereof |
| CN103392743B (en) * | 2013-08-13 | 2015-12-23 | 广东省农业科学院植物保护研究所 | Bactericidal composition containing bacillus and echinomycin and preparation method thereof and application |
| CN107094797A (en) * | 2017-03-17 | 2017-08-29 | 南京工业大学 | Process for preparing biological pesticide by compounding bacillus cereus and antibiotics |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1116800C (en) | 2003-08-06 |
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Granted publication date: 20030806 |
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