CN103392743B - Bactericidal composition containing bacillus and echinomycin and preparation method thereof and application - Google Patents
Bactericidal composition containing bacillus and echinomycin and preparation method thereof and application Download PDFInfo
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- CN103392743B CN103392743B CN201310351827.2A CN201310351827A CN103392743B CN 103392743 B CN103392743 B CN 103392743B CN 201310351827 A CN201310351827 A CN 201310351827A CN 103392743 B CN103392743 B CN 103392743B
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Abstract
The present invention discloses a kind of bactericidal composition containing bacillus and echinomycin and preparation method thereof and application.This bactericidal composition is made up of the biologic product containing bacillus PPRI78 and echinomycin wettable powder.Bacillus PPRI78 thalline and solid powder are mixed, obtains the biologic product containing bacillus PPRI78; Echinomycin is mixed with filler, auxiliary agent, stirs and pulverize, obtain echinomycin wettable powder; Biologic product containing bacillus PPRI78 and echinomycin wettable powder are stirred, dry, pulverize, obtain the bactericidal composition containing bacillus and echinomycin.This bactericidal composition can effectively control the harm such as Phytophthora capsici disease, pineapple heart rot at short notice, and bacillus vegetables as root system environment in have and stronger determine to grow and fertility, can effectively reduce the incidences of disease such as Phytophthora capsici disease, pineapple heart rot, reach the effect of long-term control disease.
Description
Technical field
The invention belongs to plant protection and biological pesticide technical field, particularly a kind of bactericidal composition containing bacillus and echinomycin and preparation method thereof and application.
Background technology
Along with modern agriculture is to the efficiency utilization in soil, its cropping pattern (continuous cropping, multiple cropping etc.), makes pathogen radix in soil accumulate year by year, wherein vegetable plague frequent outbreak of epidemic and cause serious financial consequences under suitable weather conditions.
Planting family some efficient germicides of Reusability aborning very easily makes epidemic disease bacterium develop immunity to drugs, and as now widely used bactericide (metalaxyl, white urea cyanogen etc.), has occurred pesticide resistance in various degree.Therefore, be necessary to study the new reactive compound effectively preventing and treating epidemic disease bacterium.
Biocontrol microorganisms can suppress the growth of pathogenic microorganism to a certain extent, thus reduces the incidence of disease of crop.Adaptable to environment of Biocontrol Bacillus, certain quantity can be kept very well in various natural, ecological, can breed rapidly under the good condition of nutritional condition, there is stronger competitiveness in the ecosystem, be be developed to biocontrol fungicide major microorganisms source at present, and Biocontrol Bacillus have lasting control action to disease.
Research Biocontrol Bacillus and novel active compound combination control plant disease, can reach the effect of a short-term and long-term control epidemic disease, have very important using value to vegetables No-harmful apple orchard.
Summary of the invention
Primary and foremost purpose of the present invention is that the shortcoming overcoming prior art is with not enough, provides a kind of bactericidal composition containing bacillus and echinomycin.
Another object of the present invention is to provide the described preparation method containing the bactericidal composition of bacillus and echinomycin.
Another object of the present invention is to provide the described application containing the bactericidal composition of bacillus and echinomycin.
Object of the present invention is achieved through the following technical solutions: a kind of bactericidal composition containing bacillus and echinomycin, is made up of the biologic product containing bacillus (Bacullicessp.) PPRI78 and echinomycin wettable powder;
Described bacillus PPRI78 is preferably mass percent 2.5% at the described content contained in the bactericidal composition of bacillus and echinomycin;
Described echinomycin is preferably mass percent 15 ~ 25% at the described content contained in the bactericidal composition of bacillus and echinomycin; Be more preferably mass percent 25%;
The described biologic product containing bacillus PPRI78 comprises bacillus PPRI78 thalline and solid powder; Preferably by bacillus PPRI78 thalline and solid powder 5:95 proportioning in mass ratio;
Described bacillus PPRI78 is preserved in the China typical culture collection center (CCTCC) being positioned at Wuhan, China on December 27th, 2010, deposit number is CCTCCNO:M2010365; This bacterial classification is open at national patent ZL201110030415.X;
The particle diameter of described solid powder is preferably less than 1 millimeter;
At least one in described solid powder preferably talc, kaolin, bentonite, diatomite, potter's clay or wheat bran;
The described biologic product containing bacillus PPRI78 prepares as follows: bacillus Bacullicessp.PPRI78 thalline and solid powder are mixed and obtain;
Described echinomycin wettable powder comprises echinomycin, auxiliary agent and filler; Preferably comprise the echinomycin of mass percent 30 ~ 50%, the auxiliary agent of mass percent 10%, the filler of mass percent 27.5 ~ 52.5%;
The concentration of described echinomycin in described echinomycin wettable powder is more preferably 50%;
Described echinomycin is preferably the echinomycin reagent of commercially available mass percent 80%, and now, described echinomycin wettable powder is preferably grouped into by following one-tenth by mass percentage:
Concentration is the echinomycin reagent 37.5 ~ 62.5% of mass percent 80%;
Auxiliary agent 10%
Filler 27.5 ~ 52.5%;
More preferably be grouped into by following one-tenth by mass percentage:
Concentration is the echinomycin reagent 62.5% of mass percent 80%;
Auxiliary agent 10%
Filler 27.5%;
Described auxiliary agent is preferably one or both in methoxyl group fatty acyl amido benzene sulfonic acid sodium salt and white carbon;
Described filler is at least one in precipitated calcium carbonate, kaolin and diatomite;
Described echinomycin wettable powder prepares preferably by following method:
(1) echinomycin is mixed with filler, stir;
(2) material that step (1) obtains is mixed with auxiliary agent, through stirring and pulverizing, obtain echinomycin wettable powder;
Wherein, the mass percentage of echinomycin (active ingredient) in wetting powder is 30 ~ 50%;
The described preparation method containing the bactericidal composition of bacillus and echinomycin, comprises following steps:
I, bacillus PPRI78 thalline and solid powder to be mixed, obtain the biologic product containing bacillus PPRI78;
II, echinomycin to be mixed with filler, stir; Add auxiliary agent mixing again, through stirring and pulverizing, obtain echinomycin wettable powder;
The echinomycin wettable powder mixing that III, the biologic product containing bacillus PPRI78 obtained by step I and Step II obtain, stirs, dry, pulverize, obtain the bactericidal composition containing bacillus and echinomycin;
Bacillus PPRI78 thalline described in step I and described solid powder be 5:95 mixing in mass ratio preferably;
Echinomycin wettable powder described in Step II comprises following composition by mass percentage: echinomycin 30 ~ 50%, auxiliary agent 10%, filler 27.5 ~ 52.5%;
The biologic product containing bacillus PPRI78 described in Step II I and described echinomycin wettable powder be 1:1 proportioning mixing in mass ratio preferably;
The condition of the drying described in Step II I is preferably temperature not higher than 70 DEG C, is dried to moisture relative moisture and is less than mass percent 10%;
The described bactericidal composition containing bacillus and echinomycin is preventing and treating the application in plant Oomycete disease.
Described oomycetes is preferably phytophthora, is more preferably at least one in P. capsici and the former bacterium of pineapple heart rot.
The present invention has following advantage and effect relative to prior art:
(1) bactericidal composition containing bacillus and echinomycin provided by the invention, bacillus PPRI78 and echinomycin collocation are used, particularly the concentration of bacillus PPRI78 is mass percent 2.5%, when the concentration of echinomycin is mass percent 25%, can effectively control the harm such as Phytophthora capsici disease, pineapple heart rot at short notice, and bacillus biologic product vegetables as root system environment in have and stronger determine to grow and fertility, can effectively reduce the incidences of disease such as Phytophthora capsici disease, pineapple heart rot, reach the effect of long-term control disease.
(2) echinomycin is a kind of quinoxaline antibiotic, and (shown in I, molecular formula is C to have more novel chemical constitution
51h
64n
12o
12s
2, molecular weight is 1101).Inventor's Late Cambrian echinomycin to oomycetes, particularly the mycelial growth of epidemic disease bacterium, sporangium is formed and spore germination has obvious inhibitory action, and has good control efficiency to epidemic disease bacterium, not easily develop immunity to drugs.
(3) echinomycin wettable powder of the present invention mature preparation process, be easy to operation, production cost is low.Itself and bacillus biologic product can fully mix, and stable in properties can be preserved in room temperature after sealing for a long time.
Accompanying drawing explanation
Fig. 1 is the suppression result figure of echinomycin to phytophthora capsici mycelial growth, and from left to right, more from top to bottom, echinomycin concentration is respectively 400 μ g/mL, 200 μ g/mL, 100 μ g/mL, 50 μ g/mL, 25 μ g/mL, and last is contrast CK.
Fig. 2 is the suppression result figure of echinomycin to pineapple heart rot bacterium mycelial growth, from left to right, again from top to bottom, echinomycin concentration is respectively 160 μ g/mL, 80 μ g/mL, 40 μ g/mL, 20 μ g/mL, 10 μ g/mL, 5 μ g/mL, 2.5 μ g/mL, 1.25 μ g/mL, and last is contrast CK.
Fig. 3 is the suppression result figure that echinomycin is formed phytophthora capsici sporangium, and from left to right, more from top to bottom, echinomycin concentration is respectively 0.1 μ g/mL, 0.2 μ g/mL, 0.4 μ g/mL, 0.8 μ g/mL, 1.6 μ g/mL, and last is contrast CK.
Fig. 4 is the suppression result figure of echinomycin to phytophthora capsici spore germination, and from left to right, more from top to bottom, echinomycin concentration is respectively 0.2 μ g/mL, 0.4 μ g/mL, 0.8 μ g/mL, 1.6 μ g/mL, 3.2 μ g/mL, and last is contrast CK.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1 contains the preparation of the bactericidal composition of bacillus and echinomycin
The preparation of (one) 50% echinomycin wettable powder
(1) get 27.5g kaolin to drop in miniature mix and blend instrument, start and stir instrument stirring, progressively add 62.5g gram mass percentage composition 80% echinomycin (being purchased from Bo Sheng bio tech ltd, Shanghai), make the two fully mixing;
(2) material step (1) obtained and 10g stir in crusher to dropping into together with methoxyl group fatty acyl amido benzene sulfonic acid sodium salt, stir and pulverize; Pulverize through miniature air stream crusher, obtain 100 grams of echinomycin wettable powder, it is 50% that echinomycin content accounts for quality of the pharmaceutical preparations ratio again.
(2) preparation of bacillus PPRI78 biologic product
(1) activation of bacterial classification: preparation NA medium (often liter consist of: beef extract 3g, yeast extract 1g, peptone 5g, glucose 10g, water is settled to 1000mL, pH is 7.2 ~ 7.4) after, each specification is fill NA medium 100mL in the triangular flask of 500mL, 121 sterilizing 15min.Cultivation bacterium 1 ring after cooling on access bacillus PPRI78(flat board, this bacterial classification is preserved on December 27th, 2010 China typical culture collection center being positioned at Wuhan, China, and deposit number is CCTCCNO:M2010365; This bacterial classification is open at national patent ZL201110030415.X), put into shaking table fermented and cultured, temperature 28 DEG C, rotating speed is 200 turns/min, incubation time is 16 hours, obtain the bacterium liquid activated;
(2) preparation of seed liquor: the fermentation tank of application 100L ferments, and zymotic fluid is LA medium (often liter consists of: corn starch 3g, soyabean protein powder 15g, corn steep liquor 25g, MnSO
40.0308mg, K
2hPO
43g, KH
2pO
41.5g, MgSO
40.5g, FeSO
40.1g, CaCO
31g, water is settled to 1000mL, pH7.0), liquid amount is 70L, and the bacterium liquid 5L of activation step (1) obtained is seeded to 100L fermentation tank, and bubble enemy's amount (percent by volume) is 0.05%, and initial pH is 7.2, fermentation temperature 30 DEG C, fermented and cultured 24 hours;
(3) amount reproduction of thalline: application 1000L biological fermentation tank ferments, zymotic fluid is LA medium, liquid amount is 700L, the bacterial classification 70L of the activation that inoculation step (2) obtains, bubble enemy's amount (percent by volume) is 0.05%, and initial pH is 7.2, fermentation temperature 30 DEG C, fermented and cultured 36 hours, period samples microscopy thalline number respectively, and thalline is not yet aging;
(4) biologic product preparation: zymotic fluid is carried out centrifugal in room temperature, 20000rpm, obtain thalline and particle diameter be less than l millimeter solid powder (by talcum, kaolin and bentonite in mass ratio 1:1:1 be obtained by mixing) in mass ratio 5:95 mix, thalline content accounts for mass percent 5%, then fluid-bed drying, the condition of fluid-bed drying is that temperature is not higher than 70 DEG C, be dried to moisture relative moisture to be less than mass percent 10%, to pulverize, obtain the biologic product containing bacillus PPRI78.
(3) preparation of the bactericidal composition containing bacillus and echinomycin
Getting 50 grams of echinomycin concentration prepared by the biologic product (thalline content is mass percent 5%) containing bacillus PPRI78 prepared by 50 grams of steps (two) and step () is that the echinomycin wettable powder of mass percent 50% drops in miniature mix and blend instrument, start and stir instrument stirring, then fluid-bed drying, the condition of fluid-bed drying is that temperature is not higher than 70 DEG C, be dried to moisture relative moisture and be less than mass percent 10%, pulverize, obtain the bactericidal composition containing bacillus and echinomycin, wherein the concentration of bacillus PPRI78 is mass percent 2.5%, the concentration of echinomycin is mass percent 25%.
Effect example 1 echinomycin is on the impact of phytophthora capsici and pineapple heart rot bacterium mycelial growth
By phytophthora blight of pepper (PhytophthoracapsiciLeonian) (" Shen Huifang etc., capsicum variety is to the Resistance Identification of epidemic disease. guangdong agricultural science, 2012,1:46 ~ 47 " open) at V8 solid culture medium (imported from America V
8vegetable juice 150mL, CaCO
30.2g, agar 18g, water 850mL) on plate 26 ~ 28 DEG C of shadings cultivate 5d, bacterium cake is got at colony edge with card punch (Φ=6mm), in the middle of V8 liquid nutrient medium (being only not containing agar with the difference of the V8 solid culture medium) plate being placed on different echinomycin concentration, with without medicine medium for contrasting.When phytophthora blight of pepper covers with mycelia in without medicine medium, measure colony diameter (as shown in Figure 1) in the V8 solid culture medium plate of different echinomycin concentration, calculate toxicity regression curve and the EC of echinomycin inhibiting rate mycelial growth
50, EC
95value.Make effect Phytophthora nicotianae (Phytophthoranicotianae) (open at " ShenHF; etal.FirstReportofPineappleHeartRotCausedbyPhytophthoran icotianaeinHainanProvince; China.PlantDisease; 2013; 97 (4): 560 ") that uses the same method, it is the main pathogenic fungi causing pineapple heart rot, and result as shown in Figure 2.
Calculating the toxicity regression curve of echinomycin to phytophthora capsici mycelial growth is: Y=6.0880+2.1831X; Correlation coefficient (r)=0.9763; EC
50=0.32 μ g/mL; EC
95=1.80 μ g/mL;
Calculating the toxicity regression curve of echinomycin to pineapple heart rot bacterium mycelial growth is: Y=5.1298+2.1062X; Correlation coefficient (r)=0.9667; EC
50=0.87 μ g/mL; EC
95=5.24 μ g/mL.
Visible, echinomycin effectively can suppress phytophthora capsici and pineapple heart rot bacterium mycelial growth.
The impact that effect example 2 echinomycin is formed phytophthora capsici sporangium
5d is cultivated in phytophthora blight of pepper 26 ~ 28 DEG C of shadings on V8 solid culture medium plate, and getting bacterium cake with card punch (Φ=6mm) at colony edge, be placed in the liquid containing the different echinomycin concentration of 20ml, take sterile water as contrast.Be placed in 28 DEG C of illumination box continuous illumination 48h, then under 10 × 10 power microscopes, detect sporangium quantity (as shown in Figure 3).Each bacterium cake random detection 4 looks eye.Often process repetition 3 times, calculate toxicity regression curve and EC that echinomycin formed sporangium
50, EC
95value.
Calculating echinomycin to the toxicity regression curve that phytophthora capsici sporangium is formed is: Y=6.7192+3.0155X; Correlation coefficient (r)=0.9852; EC
50=0.27 μ g/mL; EC
95=0.94 μ g/mL.
Visible, echinomycin can effectively suppress phytophthora capsici sporangium to be formed.
Effect example 3 echinomycin is on the impact of phytophthora capsici spore germination
After by phytophthora blight of pepper, on V8 solid culture medium plate, 5d is cultivated in 26 ~ 28 DEG C of shadings, transfer to lower 28 DEG C of illumination condition and cultivate 48h, it is induced to produce sporangium, in culture dish, add 10mL sterile water be placed in and move to room temperature after 4 DEG C of refrigerators process about 30min and place, after light treatment 2h, Zoospore liberation in sporangium, filters and obtains zoospore suspension, measure spore suspension miospore concentration with blood counting chamber, and be diluted to about 4000cfu/mL.Get a certain amount of that zoospore suspension mixes with the V8 liquid medium of equal-volume pastille, dark culturing 8h under 26 ~ 28 DEG C of conditions, measurement microscope zoospore germination rate (as shown in Figure 4), often processes repetition 3 times, calculates echinomycin to the inhibiting rate of spore germination and toxicity regression curve and EC
50, EC
95value.
Calculating echinomycin to the toxicity regression curve that phytophthora capsici sporangium is formed is: Y=6.2486+1.7876X; Correlation coefficient (r)=0.9654; EC
50=0.20 μ g/mL; EC
95=1.67 μ g/mL.
Visible, echinomycin can effectively suppress phytophthora capsici spore germination.
Effect example 4
(1) the potted plant control efficiency of composition to capsicum epidemic disease of bacillus and echinomycin is tested
Pot experiment is carried out in the greenhouse of 26 ~ 28 DEG C.(peat, perlite, gardening vermiculite by volume 1:1:1 mix for each plastic basin alms bowl dress natural air drying vegetable garden soil 0.4kg and matrix.) 0.1kg, 4 leaf phases healthy and strong Hot Pepper Seedling 2 ~ 3 transplanted by every alms bowl, transplants its growth after 5 days and normally tests, and now adds 50g wheat bran in every basin, and covers with the soil in basin alms bowl.
P. capsici cultivates 7 ~ 9d upper 26 DEG C of V8 solid culture medium plate (diameter 9cm), when microscopic examination has zoosporangium to be formed, sterile water is added in plate, put into 4 DEG C of refrigerator process after 30 minutes, be placed in room temperature lower 10 minutes, scrape hyphal surface gently with spreading rod, zoospore is fully discharged, blood counting chamber calculates zoospore quantity, and is diluted to zoospore concentration 10
3~ 10
4about cfu/mL.
If: 1. control treatment (CK), does not namely add any medicament and processes; 2. contrasting medicament is 50% echinomycin wettable powder (i.e. 50% echinomycin wettable powder for preparing of embodiment 1 step (), called after contrast medicine 1), (Zibo, Shandong Province Lv Jing agricultural chemicals Co., Ltd produces 50% dimethomorph wetting powder, called after contrast medicine 2) and 5% bacillus biologic product (the i.e. preparation prepared of embodiment 1 step (two), called after contrast medicine 3), wherein, contrast medicine 1 and contrast medicine 2 dilute 1000 times and 2000 times of uses by mass volume ratio respectively, and contrast medicine 3 dilutes 1000 times by mass volume ratio; 3. treatment agent is that bactericidal composition containing bacillus and echinomycin (i.e. embodiment 1 step (three) prepare preparation) dilutes 1000 times and 2000 times by mass volume ratio respectively.
During test, hinder root process Hot Pepper Seedling, then respectively process blade and the base portion of reagent spray Hot Pepper Seedling, spray value is as the criterion to drip, and dries, and it is 10 that every basin adds 10mL concentration
3~ 10
4cfu/mL Phytophthora capsici zoospore.Every concentration process 30 basin.Moisturizing process 24 hours after process, 7,10,21 days investigation morbidity numbers after process, calculate the incidence of disease and control efficiency, result is as shown in table 1.
The results from pot experiment test of table 1 pair capsicum epidemic disease
Note: control efficiency (%)=(the contrast incidence of disease-treatment region incidence of disease)/check plot incidence of disease × 100%
As shown in Table 1, the control efficiency that the bactericidal composition containing bacillus and echinomycin has had capsicum epidemic disease.Dispenser process is after 21 days, wherein dilute 1000 times of potted plant control efficiency to capsicum epidemic disease all more than 84%, be better than and contrast medicament 50% echinomycin wettable powder and 50% dimethomorph wettable powder dilution agent 1000 times and 2000 times and 5% bacillus biologic product and dilute 1000 times of potted plant control efficiency to capsicum epidemic disease.
(2) the potted plant control efficiency of composition to pineapple heart rot of bacillus and echinomycin is tested
Pot experiment is carried out in the greenhouse of 26 ~ 28 DEG C.(peat, perlite, gardening vermiculite by volume 1:1:1 mix for each plastic basin alms bowl dress natural air drying vegetable garden soil 0.8kg and matrix.) 0.2kg, healthy and strong pineapple seedling 1 (height of seedling 25 ~ 30cm) transplanted by every alms bowl, transplants its growth after 7 days and normally tests, and now adds 50g wheat bran in every basin, and covers with the soil in basin alms bowl.
Phytophthora nicotianae cultivates 7 ~ 9d upper 26 DEG C of V8 solid culture medium plate (diameter 9cm), when microscopic examination has zoosporangium to be formed, sterile water is added in plate, put into 4 DEG C of refrigerator process after 30 minutes, be placed in room temperature lower 10 minutes, scrape hyphal surface gently with spreading rod, zoospore is fully discharged, blood counting chamber calculates zoospore quantity, and is diluted to zoospore concentration 10
3~ 10
4about cfu/mL.
If: 1. control treatment (CK), does not namely add any medicament and processes; 2. contrasting medicament is 50% echinomycin wettable powder (i.e. 50% echinomycin wettable powder for preparing of embodiment 1 step (), called after contrast medicine 1), (Zibo, Shandong Province Lv Jing agricultural chemicals Co., Ltd produces 50% dimethomorph wetting powder, called after contrast medicine 2) and 5% bacillus biologic product (the i.e. preparation prepared of embodiment 1 step (two), called after contrast medicine 3), wherein, contrast medicine 1 and contrast medicine 2 dilute 1000 times and 2000 times of uses by mass volume ratio respectively, and contrast medicine 3 dilutes 1000 times by mass volume ratio; 3. treatment agent is that bactericidal composition containing bacillus and echinomycin (i.e. embodiment 1 step (three) prepare preparation) dilutes 1000 times and 2000 times by mass volume ratio respectively.
During test, hinder root process pineapple seedling, then respectively process lower blade and the base portion of reagent spray pineapple, spray value is as the criterion to drip, and dries, and it is 10 that every basin adds 10mL concentration
3~ 10
4cfu/mL pineapple heart rot pathogen zoospore.Every concentration process 30 basin.Moisturizing process 24 hours after process, 7,12,21 days investigation morbidity numbers after process, calculate the incidence of disease and control efficiency, result is as shown in table 2.
The results from pot experiment test of table 2 pair pineapple heart rot
Note: control efficiency (%)=(the contrast incidence of disease-process incidence of disease)/contrast incidence of disease × 100%
As shown in Table 2, the bactericidal composition containing bacillus and echinomycin has good control efficiency to pineapple heart rot.Dispenser process is after 21 days, dilute 1000 times of potted plant control efficiency to pineapple heart rot all more than 73%, with to contrast medicament 50% dimethomorph wettable powder dilution agent 1000 doubly suitable to the potted plant control efficiency of pineapple heart rot, be better than 50% echinomycin wettable powder and dilute the preventive effect that 1000 times and 2000 times and 5% bacillus biologic product dilute 1000 times.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1. contain a bactericidal composition for bacillus and echinomycin, it is characterized in that: be made up of the biologic product containing bacillus (Bacillussp.) PPRI78 and echinomycin wettable powder;
The 1:1 proportioning mixing in mass ratio of the described biologic product containing bacillus PPRI78 and described echinomycin wettable powder;
Described bacillus PPRI78 is mass percent 2.5% at the described content contained in the bactericidal composition of bacillus and echinomycin;
Described echinomycin is mass percent 15 ~ 25% at the described content contained in the bactericidal composition of bacillus and echinomycin;
The described biologic product containing bacillus PPRI78 comprises bacillus PPRI78 thalline and solid powder;
Described echinomycin wettable powder comprises echinomycin, auxiliary agent and filler;
The described biologic product containing bacillus PPRI78 by bacillus PPRI78 thalline and solid powder in mass ratio 5:95 proportioning obtain;
Described echinomycin wettable powder comprises following composition by mass percentage: echinomycin 30 ~ 50%, auxiliary agent 10%, filler 27.5 ~ 52.5%.
2. the bactericidal composition containing bacillus and echinomycin according to claim 1, is characterized in that:
Described solid powder is at least one in talcum, kaolin, bentonite, diatomite, potter's clay or wheat bran;
Described auxiliary agent is to one or both in methoxyl group fatty acyl amido benzene sulfonic acid sodium salt and white carbon;
Described filler is at least one in precipitated calcium carbonate, kaolin and diatomite.
3. the preparation method containing the bactericidal composition of bacillus and echinomycin described in claim 1 or 2, is characterized in that comprising following steps:
I, bacillus PPRI78 thalline and solid powder to be mixed, obtain the biologic product containing bacillus PPRI78;
II, echinomycin to be mixed with filler, stir; Add auxiliary agent mixing again, through stirring and pulverizing, obtain echinomycin wettable powder;
The echinomycin wettable powder mixing that III, the biologic product containing bacillus PPRI78 obtained by step I and Step II obtain, stirs, dry, pulverize, obtain the bactericidal composition containing bacillus and echinomycin.
4. the preparation method of the bactericidal composition containing bacillus and echinomycin according to claim 3, is characterized in that: the condition of the drying described in Step II I be temperature not higher than 70 DEG C, be dried to moisture relative moisture and be less than mass percent 10%.
5. the bactericidal composition containing bacillus and echinomycin described in claim 1 or 2 is preventing and treating the application in plant Oomycete disease, it is characterized in that: described oomycetes is phytophthora.
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