CN117462462A

CN117462462A - Method for producing mask by fermenting white birch juice by using microorganism strain

Info

Publication number: CN117462462A
Application number: CN202311380894.7A
Authority: CN
Inventors: 趙南奭; 金珍英; 刘培玉; 孔明霞
Original assignee: Hyundai Bioland Jiangsu Co Ltd
Current assignee: Hyundai Bioland Jiangsu Co Ltd
Priority date: 2023-10-24
Filing date: 2023-10-24
Publication date: 2024-01-30

Abstract

The invention relates to the technical field of cosmetics and discloses a method for producing a mask by fermenting white birch juice by utilizing a microbial strain, which comprises the steps of fermenting and culturing Acetobacter xylinus strain I and Lactic acid bacteria strain II, adding white birch juice in the strain fermentation process, utilizing the growth metabolism of the white birch juice to directly obtain a complete cellulose white birch juice mask, washing the mask to be neutral by weak base solution, washing the redundant weak base solution by water, and soaking the mask in a preservative. The facial mask disclosed by the invention has good beautifying effect, good whitening and moisturizing effects, super-strong skin-friendly property, good softness and elasticity, high biocompatibility, good biodegradability and excellent water holding capacity, and also has the beautifying effects of whitening, moisturizing, resisting bacteria, diminishing inflammation, removing dead skin and enabling skin to be smooth and tender.

Description

Method for producing mask by fermenting white birch juice by using microorganism strain

Technical Field

The invention relates to the technical field of cosmetics, in particular to a method for producing a mask by fermenting white birch juice by utilizing a microbial strain.

Background

With the progress of the age and the development of economy, people are focusing on the maintenance of images, wherein the cleaning and protection of the faces are particularly important, and the facial masks are favored by more and more lovers as cosmetics for cleaning and protecting the skin of the faces.

The facial mask can solve five skin problems simultaneously: whitening, moisturizing, controlling oil, anti-aging and anti-sensitivity. However, other cosmetics focus on a single problem and cannot fully meet the needs of people. The facial mask is covered on the facial skin for a short time to isolate external air and pollution, expand pores, promote sweat gland secretion and metabolism, increase skin elasticity, and the effective components in the facial mask can adsorb dirt on the skin surface and in the pores to keep the face clean.

The mask cloth in the current market is made of non-woven fabrics, hydrogel, pure cotton fibers, biological fibers and the like. The non-woven fabric mask is the most common mask on the market, but the non-woven fabric has poor affinity with skin, can be used as a carrier of mask liquid only, and the discarded mask can generate serious environmental pollution. Therefore, the research and development of the novel mask material has great practical value.

Birch (Betula Platyphylla) is a common name of species of Betula, which is the most common one of Betula plants of Betulaceae, and the area of the Betula woods in China is approximately 500 ten thousand hectares, and about 20-60 tons of Betula juice can be produced per hectare each year. At present, research on development and utilization of birch juice resources in China is continuously emerging, and the birch juice resources are applied to the fields of drinks and medicines and gradually appear in the fields of beauty and skin care. The birch juice contains rich natural proteins and nutrients, the birch juice and fermentation broth are rich in nutrients, amino acids, fatty acids, total polyphenols, total sugar and other substances, organic acids and derivatives thereof, and also contains some phenylpropanoid polyketides, lignin and other physiological active substances; the birch juice and the fermentation liquor are safe and nonirritating to human skin, and in-vitro biochemical experiment results show that the birch juice and the fermentation liquor have certain free radical scavenging capacity and better anti-saccharification effect, and are cosmetic raw materials with development potential and application value.

The method for producing the mask by fermenting the microorganism strain disclosed in CN106265474B adopts a single KOMAGATAEIBACTER RHAETICUS strain for simple activation and passage, and has poor whitening effect.

Therefore, a new solution is needed to solve the above-mentioned problems.

Disclosure of Invention

The technical problem to be solved by the invention is to provide the method for producing the mask by fermenting the white birch juice by using the microbial strain, which has the advantages of excellent adhesion, high water-retaining capacity, good biocompatibility, good biodegradability, no stimulation and good whitening effect.

In order to solve the technical problems, the invention provides a method for producing a mask by fermenting white birch juice by using a microorganism strain, which comprises the following steps:

(1) Preparation of liquid culture Medium

The first liquid culture medium consists of the following components in percentage by mass: glucose 1.0-3.0%, yeast extract 0.5-1.5%, peptone 0.3-0.7%, sodium pyrophosphate 0.3-0.7%, and balance pure water, wherein the amount of conical bottled liquid is 1/5-1/3 of that of conical bottle, and the bottle is sealed by cotton plug; sterilizing the above components with high pressure steam at 115-121deg.C for 0-40min; the first medium provides nutrients required for the activation of species i.

The second liquid culture medium consists of the following components in percentage by mass: MRS culture medium 5% -6%, balance pure water, conical bottled liquid amount 2/3-4/5 of conical flask, and cotton plug sealing; sterilizing the above components with high pressure steam at 115-121deg.C for 0-40min; the second medium provides nutrients required for the activation of strain II.

The third liquid culture medium consists of the following components in percentage by mass: glucose 0.5-1.5%, sodium acetate 0.1-0.2%, and birch sap in balance, regulating pH to 3.95-4.05 with glacial acetic acid, and sterilizing strain II with high pressure steam at 115-121deg.C for 0-30min; the third culture medium is suitable for strain growth and fermentation, provides nitrogen source carbon source, inorganic salt and acid-base environment required by strain fermentation, and the addition of the white birch sap provides sugar, amino acid, vitamin and trace elements, thereby being beneficial to microbial growth and fermentation and further producing the cellulose mask.

(2) Fermenting white birch juice by using a microbial strain to produce a mask:

a. activating strains:

a-1: thawing Acetobacter xylinus strain I at normal temperature, adding into the first liquid culture medium, shake culturing at 25-30deg.C and 170-190rpm for 36-54 hr;

a-2: thawing Lactic acid bacteria strain II at normal temperature, adding into a second liquid culture medium, standing at 35-40deg.C, and culturing for 24-36 hr;

b. and (5) strain passage:

after Acetobacter xylinus strain I is activated, 5-15% of activated strain by mass percentage is put into a first liquid culture medium, and shake cultivation is carried out for 18-30h at 25-30 ℃ at 170-190 rpm;

activating Lactic acid bacteria strain II, taking 5-15% of activated strain by mass percentage, placing the activated strain into a second liquid culture medium, and standing and culturing for 15-20h at 35-40 ℃;

the liquid culture mediums adopted for strain passage are the repetition of the first liquid culture medium and the second liquid culture medium respectively, and the cost is reduced by increasing the strain inoculation amount and reducing the culture time.

c. And (3) strain fermentation:

after strain passage, strain 1 (5% -15%) and strain 2 (0.1-1%) after passage are taken into a third liquid culture medium, and the mask is taken out after standing fermentation culture for 48-72 hours at 25-30 ℃;

(3) Washing: washing the cellulose mask with weak base solution to neutrality, and washing off excessive weak base solution with water;

(4) And (3) detection: sampling the washed cellulose mask to perform parameter detection;

(5) Soaking: and (3) soaking and preserving the washed cellulose mask in a preservative at normal temperature.

Preferably, in the washing process of the step (3), the cellulose mask is prepared by removing the liquid culture medium and acetic acid smell by using a sodium percarbonate solution with the mass percentage of 0.4% -0.6%, and washing away the excessive sodium percarbonate solution by using clear water to obtain the cellulose mask without impurities and with neutral pH value.

Research shows that the natural white birch juice is rich in various vitamins, amino acids, fatty acids and microelements required by human skin, and contains antioxidant nutrient elements selenium, calcium, magnesium, potassium and other mineral substances. The birch sap has the main effects of skin conditioning agent, antioxidant, relieving and anti-allergy in cosmetics and skin care products, has a risk factor of 1, is safer, can be used safely, has no influence on pregnant women generally, has no poxiness, can inhibit the release of histamine, has anti-allergy effect, has inhibition effect on elastase and metalloproteinase, and shows that the anti-aging effect is stronger. Can be used in combination with other components to regulate skin, smooth skin, prevent chapping, and improve skin color.

Compared with the prior art, the invention has the following beneficial effects:

1. the facial mask is prepared by mixing white birch juice with Acetobacter xylinus and Lacticacid bacteria bacteria, and the white birch juice is added in the strain fermentation process, so that the facial mask combines the white birch juice and the facial mask fermented by the two strains, has good beautifying effect, has good whitening and moisturizing effects, can relax and repair, tighten and lighten skin, and enables the skin to be smooth and tender. The facial mask produced by the birch sap is composed of biological fibers, and the biological fibers are produced by natural fermentation of fungus, so that the facial mask has the advantages of excellent adhesion, high water retention capacity, good biocompatibility, good biodegradability, no stimulation and the like, and has higher economic value.

2. The repetition of the first liquid culture medium and the second liquid culture medium of the liquid culture medium adopted by the strain passage effectively reduces the cost by increasing the inoculation amount and reducing the culture time.

3. The cellulose mask which is fermented by two strains and added with the birch sap in the fermentation process is soaked and preserved by adopting the DPG preservative, so that the mask can be preserved for a long time at normal temperature, and the preservation period can be prolonged by more than 1 month compared with the preservative-free state.

Detailed Description

The present invention will be further described in detail in order to enhance understanding of the present invention, and this embodiment is only for explaining the present invention and is not to be construed as limiting the scope of the present invention.

A method for producing a mask by fermenting white birch juice with a microbial strain, comprising the steps of:

(1) Fermenting white birch juice by using a microbial strain to produce a mask: acetobacter xylinus strain I and Lactic acid bacteria strain II are simply activated, passaged, inoculated into a liquid culture medium added with white birch sap, cultured for 2-3 days, and then taken out of the mask;

(2) Washing: washing the cellulose mask with weak base solution to neutrality, and washing off excessive weak base solution with water; the mask is neutral, has no irritation and harm to skin, and is more compatible with skin.

(3) And (3) detection: and randomly sampling the washed cellulose mask, cutting, detecting parameters of thickness, maximum tension and stretching distance and calculating the stretching strength by using related equipment, and optimizing the production mask.

(4) Soaking: soaking the washed cellulose mask in DPG preservative (DPG with mass concentration of 2%, phenoxyethanol with mass concentration of 0.5% and ethylhexyl glycerol with mass concentration of 0.05%) at normal temperature. The mask can be preserved for a long time at normal temperature, and the preservation period can be prolonged by more than 1 month compared with the preservative-free state.

Example 1

A method for producing facial mask by fermenting white birch juice with microorganism strain comprises the following steps:

(1) Activating strains, passaging and fermenting: fermenting white birch juice by using a microbial strain to produce a mask: acetobacter xylinus strain I is activated, passaged, inoculated into a liquid culture medium, cultured for 3 days, and then taken out of the mask.

a. Activating strains:

taking out and preserving 10mL of strain Acetobacter xylinus strain I by a refrigerator, thawing at normal temperature, adding into the first liquid culture medium, adding 10mL of 95% ethanol (after filtering by a 0.22 μm sieve), and performing shake culture at 30deg.C and 180rpm for 50h.

b. And (5) strain passage:

20mL of the strain I activated culture solution is taken out of the conical flask, added into the first liquid culture medium, and 10mL of 95% ethanol (after filtration through a 0.22 μm sieve) with mass concentration is added, and shake-cultured at 30 ℃ for 20h at 180 rpm.

c. And (3) strain fermentation:

and taking 50mL of the strain I culture solution out of the conical flask, adding the strain I culture solution into the second liquid culture medium, stirring and mixing uniformly, pouring the mixture into a tray, and standing and fermenting at 26 ℃ for culturing for 72 hours.

(2) Washing:

taking out the formed mask, washing off redundant culture medium, and then soaking in sodium percarbonate solution with the mass concentration of 0.5% until no acetic acid taste exists.

(3) And (3) detection:

and taking out the mask, washing off excessive sodium percarbonate solution, cutting into strips with the length of 15 mm or 50mm after water pressing, measuring the thickness, detecting the maximum pulling force and the stretching distance, and calculating the stretching strength.

(4) Soaking:

taking out the mask, washing off excessive sodium percarbonate solution, and soaking in DPG preservative (DPG with mass concentration of 2%, phenoxyethanol with mass concentration of 0.5% and ethylhexyl glycerol with mass concentration of 0.05%).

The liquid culture medium used for strain activation, passage and fermentation is as follows:

first liquid medium:

10g of glucose, 5g of yeast extract, 2.5g of peptone, 2.5g of sodium pyrophosphate and 500g of pure water, adjusting the pH value of the solution to 6.0, filling into a 2L conical flask, and sealing with a cotton plug. Sterilizing with steam at 121deg.C for 30min.

Second liquid medium:

10g of glucose, 1.5g of sodium acetate, an active ingredient A and the balance of pure water, adjusting the pH value of the solution to 4.0, and sterilizing for 30min at 121 ℃ by high-pressure steam.

The effective component A is stock solution of birch sap, and the addition amounts are respectively 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, and the corresponding pure water balance is respectively 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 0%.

Example 1 mask detection, data is summarized as follows:

in comparison with the film forming detection data in example 1, the addition of the white birch sap has a good effect on improving the thickness of the mask, and 20% and 50% of the total effect are selected for further experiments.

Example 2:

(1) Activating strains, passaging and fermenting: fermenting white birch juice by using a microbial strain to produce a mask: acetobacter xylinus strain I and Lactic acid bacteria strain II are activated, passaged, inoculated into a liquid culture medium, and cultured for 3 days, and then the mask is taken out.

a. Activating strains:

a-1: taking out 10mL of the strain Acetobacter xylinus strain I from the refrigerator, thawing at normal temperature, adding into the first liquid culture medium, adding 16mL of 95% ethanol (after 0.22 μm mesh filtration), and shake culturing at 30deg.C with 180rpm for 50 hr.

a-2: taking out and preserving 10ml of strain Lactic acid bacteria strain II by a refrigerator, thawing at normal temperature, adding into a second liquid culture medium, standing at 35 ℃, activating and culturing for 30h.

b. And (5) strain passage:

b-1: 20mL of the strain I activated culture solution is taken out of the conical flask, added into the first liquid culture medium, and 8mL of 95% ethanol (after filtration through a 0.22 μm sieve) with mass concentration is added, and shake culture is carried out at 30 ℃ for 20 hours at 180 rpm.

And (3) taking 20mL of strain II activation culture solution from the conical flask, adding the strain II activation culture solution into the second liquid culture medium, and standing and culturing for 17h at 35 ℃.

c. And (3) strain fermentation:

and taking 50mL of strain I culture solution and 5mL of strain II culture solution from the conical flask, adding the strain I culture solution and the strain II culture solution into the third liquid culture medium, stirring and mixing uniformly, pouring the mixture into a tray, and standing and fermenting at 26 ℃ for culturing for 72h.

(2) Washing:

(3) And (3) detection:

(4) Soaking:

first liquid medium:

glucose 8g, yeast extract 4g, peptone 2g, sodium pyrophosphate 2g, pure water 400g, adjusting pH to 6.0, placing into a 2L conical flask, and sealing with a cotton plug. Sterilizing with steam at 121deg.C for 30min.

Second liquid medium:

MRS broth 11.2g, pure water 200g, put into 250mL Erlenmeyer flask, and tamponade sealed. Sterilizing with steam at 121deg.C for 30min.

Third liquid medium:

glucose 10g, sodium acetate 1.5g, birch sap 200g and pure water 800g, adjusting the pH value of the solution to 4.0, and sterilizing with high pressure steam at 121 ℃ for 30min.

Example 3:

a. Activating strains:

b. And (5) strain passage:

c. And (3) strain fermentation:

(2) Washing:

(3) And (3) detection:

(4) Soaking:

first liquid medium:

Second liquid medium:

Third liquid medium:

glucose 10g, sodium acetate 1.5g, birch sap 500g, pure water 500g, adjusting pH to 4.0, and sterilizing with 121deg.C high pressure steam for 30min.

Example 4:

a. Activating strains:

b. And (5) strain passage:

c. And (3) strain fermentation:

and taking 50mL of strain I culture solution and 10mL of strain II culture solution from the conical flask, adding the strain I culture solution and the strain II culture solution into the third liquid culture medium, stirring and mixing uniformly, pouring the mixture into a tray, and standing and fermenting at 26 ℃ for culturing for 72h.

(2) Washing:

(3) And (3) detection:

(4) Soaking:

first liquid medium:

Second liquid medium:

Third liquid medium:

Example 5:

a. Activating strains:

b. And (5) strain passage:

c. And (3) strain fermentation:

(2) Washing:

(3) And (3) detection:

(4) Soaking:

first liquid medium:

Second liquid medium:

Third liquid medium:

Examples 2-5 mask detection, data are summarized as follows:

according to the data of examples 2-5, the maximum tensile force and the tensile strength of the mixed culture mask of the strain I and the strain II are obviously improved compared with those of the mixed culture mask of the strain I which is cultured independently, and in addition, the production operation difficulty, the production cost and the like are comprehensively considered, so that the mixed culture mask of the strain I and the strain II has obvious advantages.

Bacterial cellulose has many unique properties:

1. compared with plant cellulose, the bacterial cellulose has no associated products such as lignin, pectin, hemicellulose and the like, has high crystallinity (up to 95 percent, 65 percent of plant cellulose) and high polymerization degree (the polymerization degree is up to 16000 during static culture, the cotton linter is about 5000, and the wood pulp cellulose is 7000-10000.).

2. Ultra-fine network structure. The biological cellulose fiber is a 40-60 nanometer thick fiber bundle formed by combining microfibers with the diameter of 3-4 nanometers, and interweaving the microfibers to form a developed ultra-fine network structure.

3. The elastic modulus of the bacterial cellulose is several times to ten times or more than that of a general plant fiber, and the tensile strength is high.

4. Bacterial cellulose has a strong water holding capacity (water retention values, WRV), the WRV value of undried biological cellulose can be up to more than 1000%, and the water holding capacity after freeze drying can still be more than 600%.

5. Bacterial cellulose has high biocompatibility, adaptability and good biodegradability.

6. Controllability in bacterial cellulose biosynthesis.

7. The white birch juice is known as the guard of human health, the white birch juice contains vitamins, amino acids, fatty acids and mineral elements which are necessary for human beings, and the nutrients can form factors beneficial to health after being taken into the human body through metabolism, so that the treatment effect on diseases is achieved. By researching the aroma substances of the white birch liquid, the white birch liquid is found to contain nearly 70 compounds.

8. The white birch juice contains a large amount of vitamins and fatty acids, wherein vitamin E has strong antioxidation effect, and can delay aging of cells due to oxidization. Vitamin B3 is easy to be absorbed by skin, has moisturizing effect on skin, improves roughness of skin, has skin beautifying effect on acne and freckle, and simultaneously can make skin soft, smooth and elastic. Has good protection effect on scalp, reduces dandruff, and makes hair black and shiny.

9. The "Kaibao Bencao" describes: the birch sap is bitter, flat and nontoxic, so that the birch sap has medical effect, has good curative effects on clearing lung-heat, relieving cough, gout and healing, and is recorded in various countries and regions as a medicament for treating diseases. Gao Guiqing et al, by experimental analysis of birch sap fed to rabbits and mice: the birch juice has the health care effects of resisting fatigue and aging, and has certain promotion effect on the growth and development of animals, so that the birch juice is suitable to be used as a raw and auxiliary material of a working beverage. Because it contains nicotinic acid and betulina bark brain, the birch juice has the effect of astringing and smoothing skin, so that the toner, cosmetics and washing and caring products with medical effect are produced in the United states and the Su Union countries.

Therefore, the cosmetic using the fermented white birch sap mask finished product has better skin-friendly, elastic, moisturizing, anti-inflammatory, acne-removing, whitening and skin-tendering functions.

The fermented white birch juice mask is prepared from biological fibers, namely white birch juice and Acetobacter xylinus and Lactic acid bacteria bacteria, wherein the white birch juice is added in the strain fermentation process, and the biological fibers are produced by natural fermentation of the bacteria, so that the mask has the advantages of excellent adhesion, high water-retaining capacity, good biocompatibility, good biodegradability, no stimulation and the like, and has higher economic value. The birch sap added in the strain fermentation process has good beautifying effect, and can whiten and moisturize, resist bacteria and diminish inflammation, remove dead skin and make skin smooth and tender.

Claims

1. A method for producing a mask by fermenting white birch juice with a microbial strain, comprising the steps of:

(1) Preparation of liquid culture Medium

Preparing a first liquid culture medium, a second liquid culture medium and a third liquid culture medium; the third liquid culture medium consists of the following components in percentage by mass: glucose 0.5-1.5%, sodium acetate 0.1-0.2%, and birch juice for the rest;

(2) Fermenting white birch juice by using a microbial strain to produce a mask:

a. activating strains:

b. and (5) strain passage:

c. and (3) strain fermentation:

after strain passage, taking the strain I (5% -15%) and the strain II (0.1-1%) after passage into a third liquid culture medium, standing, fermenting and culturing at 25-30 ℃ for 48-72 hours, and taking out the mask;

(5) Soaking: and (3) placing the washed cellulose mask in a DPG preservative at normal temperature for soaking and preserving.

2. The method for producing a mask by fermenting white birch sap with a microbial strain according to claim 1, wherein: in the step (1), the first liquid culture medium consists of the following components in percentage by mass: glucose 1.0-3.0%, yeast extract 0.5-1.5%, peptone 0.3-0.7%, sodium pyrophosphate 0.3-0.7%, and balance pure water, wherein the amount of conical bottled liquid is 1/5-1/3 of that of conical bottle, and the cotton plug is sealed.

3. A method for producing a mask by fermenting white birch sap with a microbial strain according to claim 2, wherein: in the step (1), the components of the first liquid culture medium are subjected to high-pressure steam sterilization at 115-121 ℃ for 0-40min.

4. The method for producing a mask by fermenting white birch sap with a microbial strain according to claim 1, wherein: in the step (1), the second liquid culture medium consists of the following components in percentage by mass: MRS culture medium 5% -6%, balance pure water, conical bottled liquid amount 2/3-4/5 of conical bottle, and cotton plug sealing.

5. The method for producing a mask by fermenting white birch sap with a microbial strain according to claim 4, wherein: in the step (1), the components of the second liquid culture medium are subjected to high-pressure steam sterilization at 115-121 ℃ for 0-40min.

6. The method for producing a mask by fermenting white birch sap with a microbial strain according to claim 1, wherein: in the step (1), the pH value of the third liquid culture medium is regulated to 3.95-4.05 by adopting glacial acetic acid, and the strain II is killed by high-pressure steam at 115-121 ℃ for 0-30min.

7. The method for producing a mask by fermenting white birch sap with a microbial strain according to claim 1, wherein: and (3) in the washing process, removing the liquid culture medium and acetic acid smell from the cellulose mask by using a sodium percarbonate solution with the mass percentage of 0.4-0.6%, and washing off the excessive sodium percarbonate solution by using clear water to obtain the cellulose mask without impurities and with neutral pH value.