CN117460532A - 用于癌的治疗和/或预防的药品 - Google Patents

Abstract

特征在于一起包含或各自分开地组合包含与CAPRIN‑1蛋白质具有免疫反应性的抗体或其片段、和拓扑异构酶I抑制剂的药品，作为用于癌的治疗和/或预防的药品是有用的。

Description

用于癌的治疗和/或预防的药品

技术领域

本发明涉及使用了针对CAPRIN-1蛋白质的抗体或其片段和拓扑异构酶I抑制剂的用于癌的治疗和/或预防的药品。

背景技术

以癌细胞上的特异抗原蛋白质为靶标的各种抗体药物由于其癌特异性，而作为副作用少的癌治疗药被应用于癌治疗。例如，多种实体癌的细胞膜表面表达细胞质增殖相关蛋白1(Cytoplasmic-activation and proliferation-associateed protein 1，CAPRIN-1)，已知针对该CAPRIN-1蛋白质的抗体有望作为癌的治疗和/或预防用药物用途(专利文献1)。

近年来，临床上为了提高癌治疗药的有效性，已经使用将多种癌治疗药联合使用的治疗法作为标准的治疗法。例如，针对大肠癌联合使用伊立替康、亚叶酸(folinic acid)和5-氟尿嘧啶的治疗法，针对乳癌组合阿霉素和环磷酰胺的治疗法、或组合紫杉醇、曲妥珠单抗和帕妥珠单抗的治疗法，针对胃癌使用顺铂和5-氟尿嘧啶等多种抗癌剂进行治疗已经成为一般方法。对于以抗CAPRIN-1抗体作为有效成分的癌治疗药，已经通过与化疗剂联合使用而确认了优异的癌治疗效果(专利文献2)。但是，利用化疗剂彼此的组合的癌的治疗并不对所应用的全部癌有效，另外，虽然有时累加地提高治疗效果，但协同地大幅提高治疗效果的为数较少。

作为联合使用多种癌治疗药的癌治疗法的一个具体例，可列举嘧啶系的药剂(例如，吉西他滨)与卡铂的联合使用。吉西他滨与卡铂的联合使用也被称为CBDCA+GEM，尝试了针对非小细胞肺癌、复发了的晚期渐进性的卵巢癌、膀胱癌、乳癌、或尿路上皮癌等的治疗。卵巢癌容易转移到腹腔内、淋巴结、肝脏或肺，特别进展到III期、IV期的卵巢癌是首次治疗后2年以内复发的情况超过50％的恶性度极高的癌。针对以这样的卵巢癌为原发的转移癌使用吉西他滨与卡铂的联合使用疗法(GC)，但对于从首次化疗结束到复发的间期PFI(Platinum-Free interval)为6个月以上的患者(所谓铂类制剂敏感性的患者)复发后的治疗的奏效率为40％以上，与此相对，对于从化疗结束到复发的间期PFI小于6个月的患者(所谓铂类制剂非敏感性的患者)复发后的治疗的奏效率为10％以下，预后极差(非专利文献2)。作为针对铂类制剂敏感性的卵巢癌患者的强化疗法，承认了对吉西他滨、卡铂添加贝伐珠单抗的3剂联合使用疗法(GC-BEV)，但在该3剂联合使用的临床试验OCEANS中，得到了与GC相比，GC-BEV显著地延长了无恶化生存期PFS(Progression-free Survival)，但全生存期OS的中值无显著性差异这样的结果(非专利文献3)。

另一方面，对于铂类制剂非敏感性的患者，卡铂等铂类制剂在指南上不能使用，要选择其他治疗法。例如，作为紫杉烷类药剂的紫杉醇、多西他赛、作为蒽环素类药剂的阿霉素及其脂质体制剂、以及作为拓扑异构酶抑制剂的依托泊苷、拓扑替康等化疗剂被承认，但虽然验证了各种施与方案，但其奏效率为10～30％，得不到满意的效果(非专利文献4～7)。

近年来，针对铂类制剂非敏感性的患者，对各种化疗剂添加作为血管新生抑制剂的贝伐珠单抗的联合使用疗法被承认。在验证了贝伐珠单抗对化疗剂的追加效果的临床试验AURELIA中，PFS相对于单剂化疗的三四个月，贝伐珠单抗的联合使用显著地延长到六七个月，但关于OS未见显著性差异，没有获得延命效果(非专利文献8)。

现有技术文献

专利文献

专利文献1：WO2010/016526号

专利文献2：WO2011/096535号

非专利文献

非专利文献1：J Clin Oncol,2012,30,1879-1887

非专利文献2：Crit Rev Oncol Hematοl,2007,64,129-138

非专利文献3：J Clin Oncol,2012,30,2039-2045

非专利文献4：J Clin Oncol,1998,16,405-410

非专利文献5：J Clin Oncol,2001,19,3312-3322

非专利文献6：J Clin Oncol,2003,21,2843-2848

非专利文献7：J Clin Oncol,2008,26,890-896

非专利文献8：J Clin Oncol,2014,32,1302-1308

发明内容

发明要解决的课题

本发明的目的是提供用于治疗和/或预防在细胞表面特异性地表达CAPRIN-1蛋白质的癌的药品。

用于解决课题的手段

本发明人进行深入研究的结果发现，与癌细胞具有免疫反应性的针对CAPRIN-1蛋白质的抗体或其片段与拓扑异构酶I抑制剂的联合使用(combination)与各自的单剂治疗相比发挥极强的抗肿瘤效果，该抗肿瘤效果是使肿瘤退缩的协同效果，以及该效果比与其他拓扑异构酶抑制剂的联合使用效果显著高，从而完成了本发明。

具体地，本发明具有以下(1)～(14)的特征。

(1)用于癌的治疗和/或预防的药品，其特征在于，一起包含或各自分开地组合包含：与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段、和拓扑异构酶I抑制剂。

(2)根据(1)所述的药品，所述拓扑异构酶I抑制剂是拓扑替康或伊立替康。

(3)根据(1)或(2)所述的药品，所述抗体或其片段与CAPRIN-1蛋白质具有免疫反应性，所述CAPRIN-1蛋白质具有序列号2～30中任一偶数序列号所示的氨基酸序列、或与该氨基酸序列有80％以上的序列同一性的氨基酸序列。

(4)根据(1)～(3)的任一项所述的药品，所述抗体或其片段与在癌细胞表面上存在的CAPRIN-1蛋白质的细胞外区域具有免疫反应性。

(5)根据(1)～(4)的任一项所述的药品，所述抗体或其片段与CAPRIN-1蛋白质的部分多肽具有免疫反应性，所述CAPRIN-1蛋白质的部分多肽具有序列号31～35、296～299、308、309的任一项所示的氨基酸序列、或与该氨基酸序列有80％以上的序列同一性的氨基酸序列。

(6)根据(1)～(5)的任一项所述的药品，所述抗体是单克隆抗体或多克隆抗体。

(7)根据(1)～(6)的任一项所述的药品，所述抗体或其片段是以下(A)～(M)的任一者，

(A)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号36所示的互补决定区CDR1、序列号37所示的互补决定区CDR2、和序列号38所示的互补决定区CDR3，所述轻链可变区包含序列号40所示的互补决定区CDR1、序列号41所示的互补决定区CDR2、和序列号42所示的互补决定区CDR3；

(B)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号44所示的互补决定区CDR1、序列号45所示的互补决定区CDR2、和序列号46所示的互补决定区CDR3，所述轻链可变区包含序列号48所示的互补决定区CDR1、序列号49所示的互补决定区CDR2、和序列号50所示的互补决定区CDR3；

(C)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号52所示的互补决定区CDR1、序列号53所示的互补决定区CDR2、和序列号54所示的互补决定区CDR3，所述轻链可变区包含序列号56所示的互补决定区CDR1、序列号57所示的互补决定区CDR2、和序列号58所示的互补决定区CDR3；

(D)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号60所示的互补决定区CDR1、序列号61所示的互补决定区CDR2、和序列号62所示的互补决定区CDR3，所述轻链可变区包含序列号64所示的互补决定区CDR1、序列号65所示的互补决定区CDR2、和序列号66所示的互补决定区CDR3；

(E)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号170所示的互补决定区CDR1、序列号171所示的互补决定区CDR2、和序列号172所示的互补决定区CDR3，所述轻链可变区包含序列号173所示的互补决定区CDR1、序列号174所示的互补决定区CDR2、和序列号175所示的互补决定区CDR3；

(F)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号176所示的互补决定区CDR1、序列号177所示的互补决定区CDR2、和序列号178所示的互补决定区CDR3，所述轻链可变区包含序列号179所示的互补决定区CDR1、序列号180所示的互补决定区CDR2、和序列号181所示的互补决定区CDR3；

(G)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号182所示的互补决定区CDR1、序列号183所示的互补决定区CDR2、和序列号184所示的互补决定区CDR3，所述轻链可变区包含序列号185所示的互补决定区CDR1、序列号186所示的互补决定区CDR2、和序列号187所示的互补决定区CDR3；

(H)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号188所示的互补决定区CDR1、序列号189所示的互补决定区CDR2、和序列号190所示的互补决定区CDR3，所述轻链可变区包含序列号191所示的互补决定区CDR1、序列号192所示的互补决定区CDR2、和序列号193所示的互补决定区CDR3；

(I)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号146所示的互补决定区CDR1、序列号147所示的互补决定区CDR2、和序列号148所示的互补决定区CDR3，所述轻链可变区包含序列号149所示的互补决定区CDR1、序列号150所示的互补决定区CDR2、和序列号151所示的互补决定区CDR3；

(J)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号272所示的互补决定区CDR1、序列号273所示的互补决定区CDR2、和序列号274所示的互补决定区CDR3，所述轻链可变区包含序列号275所示的互补决定区CDR1、序列号276所示的互补决定区CDR2、和序列号277所示的互补决定区CDR3；

(K)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号290所示的互补决定区CDR1、序列号291所示的互补决定区CDR2、和序列号292所示的互补决定区CDR3，所述轻链可变区包含序列号293所示的互补决定区CDR1、序列号294所示的互补决定区CDR2、和序列号295所示的互补决定区CDR3；

(L)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号301所示的互补决定区CDR1、序列号302所示的互补决定区CDR2、和序列号303所示的互补决定区CDR3，所述轻链可变区包含序列号305所示的互补决定区CDR1、序列号306所示的互补决定区CDR2、和序列号307所示的互补决定区CDR3；

(M)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号134所示的互补决定区CDR1、序列号135所示的互补决定区CDR2、和序列号136所示的互补决定区CDR3，所述轻链可变区包含序列号137所示的互补决定区CDR1、序列号138所示的互补决定区CDR2、和序列号139所示的互补决定区CDR3。

(8)根据(1)～(7)的任一项所述的药品，所述抗体或其片段是以下(a)～(al)的任一者，

(a)重链可变区包含序列号39的氨基酸序列、且轻链可变区包含序列号43的氨基酸序列的抗体或其片段；

(b)重链可变区包含序列号47的氨基酸序列、且轻链可变区包含序列号51的氨基酸序列的抗体或其片段；

(c)重链可变区包含序列号55的氨基酸序列、且轻链可变区包含序列号59的氨基酸序列的抗体或其片段；

(d)重链可变区包含序列号63的氨基酸序列、且轻链可变区包含序列号67的氨基酸序列的抗体或其片段；

(e)重链可变区包含序列号68的氨基酸序列、且轻链可变区包含序列号69的氨基酸序列的抗体或其片段；

(f)重链可变区包含序列号70的氨基酸序列、且轻链可变区包含序列号71的氨基酸序列的抗体或其片段；

(g)重链可变区包含序列号72的氨基酸序列、且轻链可变区包含序列号73的氨基酸序列的抗体或其片段；

(h)重链可变区包含序列号74的氨基酸序列、且轻链可变区包含序列号75的氨基酸序列的抗体或其片段；

(i)重链可变区包含序列号76的氨基酸序列、且轻链可变区包含序列号77的氨基酸序列的抗体或其片段；

(j)重链可变区包含序列号78的氨基酸序列、且轻链可变区包含序列号79的氨基酸序列的抗体或其片段；

(k)重链可变区包含序列号80的氨基酸序列、且轻链可变区包含序列号81的氨基酸序列的抗体或其片段；

(l)重链可变区包含序列号82的氨基酸序列、且轻链可变区包含序列号83的氨基酸序列的抗体或其片段；

(m)重链可变区包含序列号84的氨基酸序列、且轻链可变区包含序列号85的氨基酸序列的抗体或其片段；

(n)重链可变区包含序列号86的氨基酸序列、且轻链可变区包含序列号87的氨基酸序列的抗体或其片段；

(o)重链可变区包含序列号88的氨基酸序列、且轻链可变区包含序列号89的氨基酸序列的抗体或其片段；

(p)重链可变区包含序列号90的氨基酸序列、且轻链可变区包含序列号91的氨基酸序列的抗体或其片段；

(q)重链可变区包含序列号92的氨基酸序列、且轻链可变区包含序列号93的氨基酸序列的抗体或其片段；

(r)重链可变区包含序列号94的氨基酸序列、且轻链可变区包含序列号95的氨基酸序列的抗体或其片段；

(s)重链可变区包含序列号96的氨基酸序列、且轻链可变区包含序列号97的氨基酸序列的抗体或其片段；

(t)重链可变区包含序列号98的氨基酸序列、且轻链可变区包含序列号99的氨基酸序列的抗体或其片段；

(u)重链可变区包含序列号100的氨基酸序列、且轻链可变区包含序列号101的氨基酸序列的抗体或其片段；

(v)重链可变区包含序列号102的氨基酸序列、且轻链可变区包含序列号103的氨基酸序列的抗体或其片段；

(w)重链可变区包含序列号104的氨基酸序列、且轻链可变区包含序列号105的氨基酸序列的抗体或其片段；

(x)重链可变区包含序列号106的氨基酸序列、且轻链可变区包含序列号107的氨基酸序列的抗体或其片段；

(y)重链可变区包含序列号108的氨基酸序列、且轻链可变区包含序列号109的氨基酸序列的抗体或其片段；

(z)重链可变区包含序列号110的氨基酸序列、且轻链可变区包含序列号111的氨基酸序列的抗体或其片段；

(aa)重链可变区包含序列号112的氨基酸序列、且轻链可变区包含序列号113的氨基酸序列的抗体或其片段；

(ab)重链可变区包含序列号114的氨基酸序列、且轻链可变区包含序列号115的氨基酸序列的抗体或其片段；

(ac)重链可变区包含序列号116的氨基酸序列、且轻链可变区包含序列号117的氨基酸序列的抗体或其片段；

(ad)重链可变区包含序列号118的氨基酸序列、且轻链可变区包含序列号119的氨基酸序列的抗体或其片段；

(ae)重链可变区包含序列号120的氨基酸序列、且轻链可变区包含序列号121的氨基酸序列的抗体或其片段；

(af)重链可变区包含序列号122的氨基酸序列、且轻链可变区包含序列号123的氨基酸序列的抗体或其片段；

(ag)重链可变区包含序列号124的氨基酸序列、且轻链可变区包含序列号125的氨基酸序列的抗体或其片段；

(ah)重链可变区包含序列号126的氨基酸序列、且轻链可变区包含序列号127的氨基酸序列的抗体或其片段；

(ai)重链可变区包含序列号128的氨基酸序列、且轻链可变区包含序列号129的氨基酸序列的抗体或其片段；

(aj)重链可变区包含序列号130的氨基酸序列、且轻链可变区包含序列号131的氨基酸序列的抗体或其片段；

(ak)重链可变区包含序列号132的氨基酸序列、且轻链可变区包含序列号133的氨基酸序列的抗体或其片段；

(al)重链可变区包含序列号300的氨基酸序列、且轻链可变区包含序列号304的氨基酸序列的抗体或其片段。

(9)根据(1)～(8)的任一项所述的药品，所述抗体是人抗体、人源化抗体、嵌合抗体或单链抗体。

(10)根据(1)～(9)的任一项所述的药品，所述癌是在细胞膜表面表达CAPRIN-1蛋白质的癌。

(11)根据(1)～(10)的任一项所述的药品，所述癌是卵巢癌、胆管癌、乳癌、肾癌、胰癌、大肠癌、黑素瘤、肺癌、肾细胞癌、霍奇金淋巴瘤、头颈癌、胃癌、间皮癌、结肠/直肠癌、食道癌、食管胃结合部癌、肝细胞癌、胶质母细胞瘤、尿路上皮癌、膀胱癌、子宫癌、原发性中枢神经系统淋巴瘤、原发性睾丸淋巴瘤、胆道癌、脑肿瘤、前列腺癌、白血病、淋巴瘤、肝癌、肉瘤、纤维肉瘤、肥大细胞瘤、肾上腺皮质癌、尤因肉瘤、多发性骨髓瘤、睾丸癌、甲状腺癌、基底细胞癌、佩吉特病或皮肤癌。

(12)癌的治疗和/或预防用药物组合物的药效增强剂，所述癌的治疗和/或预防用药物组合物以与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段作为有效成分，所述药效增强剂以拓扑异构酶I抑制剂作为有效成分。

(13)癌的治疗和/或预防用药物组合物的药效增强剂，所述癌的治疗和/或预防用药物组合物以拓扑异构酶I抑制剂作为有效成分，所述药效增强剂以与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段作为有效成分。

(14)用于癌的治疗和/或预防的方法，其特征在于，将与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段、和拓扑异构酶I抑制剂一起或各自分开地施与受检者。

发明的效果

本发明所涉及的针对CAPRIN-1蛋白质的抗体或其片段与拓扑异构酶I抑制剂的联合使用，与针对CAPRIN-1蛋白质的抗体或其片段单独以及拓扑异构酶I抑制剂单独相比发挥强的抗肿瘤效果。进而本发明所涉及的针对CAPRIN-1蛋白质的抗体或其片段与拓扑异构酶I抑制剂的联合使用，与其他拓扑异构酶抑制剂与针对CAPRIN-1蛋白质的抗体的联合使用相比显示强的抗肿瘤效果。因此针对CAPRIN-1蛋白质的抗体或其片段与拓扑异构酶I抑制剂的联合使用对癌的治疗、预防有效。

附图说明

图1是显示抗CAPRIN-1抗体与拓扑替康的联合使用对移植了表达CAPRIN-1的人癌细胞株BT474的NOD-SCID小鼠的抗肿瘤效果的图。参考编号1显示代替药物而施与PBS时的小鼠的肿瘤的大小，参考编号2显示施与拓扑替康低用量(3mg/kg)时的小鼠的肿瘤的大小，参考编号3显示施与拓扑替康高用量(10mg/kg)时的小鼠的肿瘤的大小，参考编号4显示施与抗CAPRIN-1抗体时的小鼠的肿瘤的大小，参考编号5显示联合施与抗CAPRIN-1抗体与拓扑替康低用量(3mg/kg)时的小鼠的肿瘤的大小，参考编号6显示联合施与抗CAPRIN-1抗体与拓扑替康高用量(10mg/kg)时的小鼠的肿瘤的大小。

图2是显示抗CAPRIN-1抗体与依托泊苷的联合使用对移植了表达CAPRIN-1的人癌细胞株BT474的NOD-SCID小鼠的抗肿瘤效果的图。参考编号7显示代替药物而施与PBS时的小鼠的肿瘤的大小，参考编号8显示施与依托泊苷低用量(5mg/kg)时的小鼠的肿瘤的大小，参考编号9显示施与依托泊苷高用量(15mg/kg)时的小鼠的肿瘤的大小，参考编号10显示施与抗CAPRIN-1抗体时的小鼠的肿瘤的大小，参考编号11显示联合施与抗CAPRIN-1抗体与依托泊苷低用量(5mg/kg)时的小鼠的肿瘤的大小，参考编号12显示联合施与抗CAPRIN-1抗体与依托泊苷高用量(15mg/kg)时的小鼠的肿瘤的大小。

具体实施方式

本发明中使用的针对CAPRIN-1蛋白质的抗体或其片段(以下，将它们总称为“抗CAPRIN-1抗体”)与拓扑异构酶I抑制剂的联合使用所产生的抗肿瘤活性如后所述，通过在生物体内调查对荷癌动物的肿瘤增殖的抑制来评价。

本说明书中，“联合使用”或“组合”是指将抗CAPRIN-1抗体与拓扑异构酶I抑制剂向同一生物体同时或设置规定的间隔作为各自独立的有效成分进行施与。其间隔可以是同时施与，也可以是30分钟后、或1小时后、3小时后、6小时后、12小时后、1天后、3天后、5天后、7天后、2周后、3周后、4周后。当抗CAPRIN-1抗体或拓扑异构酶I抑制剂在生物体内显示其活性时，可施与任一方。另外，可以先施与抗CAPRIN-1抗体，也可以先施与拓扑异构酶I抑制剂。

本发明所涉及的抗CAPRIN-1抗体可以是单克隆抗体或多克隆抗体，优选为单克隆抗体，只要本发明的抗体能够发挥抗肿瘤活性，就可以是任何种类的抗体，抗体是重组抗体、人抗体、人源化抗体、嵌合抗体、非人动物抗体。

另外，本发明中作为癌的治疗和/或预防的对象的受试者是人、宠物、家畜类、竞技用动物等哺乳动物，优选的受试者是人。

以下对于本发明所涉及的抗CAPRIN-1抗体、拓扑异构酶I抑制剂、包含它们作为有效成分的药品以及癌的治疗和/或预防方法进行说明。

＜抗CAPRIN-1抗体＞

作为与本发明中使用的抗CAPRIN-1抗体具有免疫反应性的抗原的具体例的、具有序列号2～30中的偶数序列号的任一项所示的氨基酸序列的CAPRIN-1蛋白质中，序列号6、8、10、12和14所示的氨基酸序列是犬的CAPRIN-1蛋白质的氨基酸序列，序列号2和4所示的氨基酸序列是人的CAPRIN-1蛋白质的氨基酸序列，序列号16所示的氨基酸序列是牛的CAPRIN-1蛋白质的氨基酸序列，序列号18所示的氨基酸序列是马的CAPRIN-1蛋白质的氨基酸序列，序列号20～28所示的氨基酸序列是小鼠的CAPRIN-1蛋白质的氨基酸序列，序列号30所示的氨基酸序列是鸡的CAPRIN-1蛋白质的氨基酸序列。

另外，本发明中使用的抗CAPRIN-1抗体也可以是与相对于所述序列号2～30中的偶数序列号的任一项所示的氨基酸序列有80％以上、优选为90％以上、更优选为95％以上、进一步优选为99％以上的序列同一性的CAPRIN-1蛋白质的变异体有免疫反应性的抗体。这里，“％序列同一性”是将2个序列在导入间隙或不导入间隙的情况下以形成最大的类似度(或一致度)的方式比对(排列)时，相同氨基酸(或碱基)相对于氨基酸(或碱基)的总数的百分比(％)。

本发明中抗CAPRIN-1抗体是指与CAPRIN-1蛋白质的全长或其片段具有免疫反应性的抗体或其片段。这里，“免疫反应性”是指在生物体内抗体与CAPRIN-1蛋白质或其部分多肽结合的特性。

本发明中使用的抗CAPRIN-1抗体可以是单克隆抗体或多克隆抗体。

与CAPRIN-1蛋白质的全长或其片段具有免疫反应性的多克隆抗体(抗CAPRIN-1多克隆抗体)可以通过例如将天然的CAPRIN-1蛋白质、或与GST等的融合蛋白质、或其部分肽免疫小鼠、产生人抗体的小鼠、大鼠、兔、鸡等，获得血清。将所得的血清通过例如硫酸铵沉淀、蛋白A、蛋白G、DEAE离子交换柱、偶联了CAPRIN-1蛋白质或部分肽的亲和柱等而获得。

关于上述免疫中使用的CAPRIN-1蛋白质的全长或其片段，CAPRIN-1及其同源物的碱基序列和氨基酸序列例如可以通过登录GenBank(美国NCBI)，利用BLAST、FASTA等算法(Karlin and Altschul，Proc.Natl.Acad.Sci.USA，90：5873-5877，1993；Altschul etal.，Nucleic Acids Res.25：3389-3402，1997)来得到。另外，其CAPRIN-1蛋白质的制作方法可以通过参照WO2014/012479获得，也可以使用表达CAPRIN-1蛋白质的细胞等。

与CAPRIN-1蛋白质的全长或其片段具有免疫反应性的单克隆抗体(抗CAPRIN-1单克隆抗体)例如可以通过将表达CAPRIN-1的乳癌细胞SK-BR-3、CAPRIN-1蛋白质的全长或其片段等施与小鼠进行免疫，将从该小鼠分离的脾脏细胞与骨髓瘤细胞，从所得的融合细胞(杂交瘤)选择产生抗CAPRIN-1单克隆抗体的克隆，从而获得。由所选择的杂交瘤产生的抗体可以通过与前述的多克隆抗体的纯化方法同样的方法获得。

本发明中使用的抗体包含人抗体、人源化抗体、嵌合抗体、非人动物抗体。

人抗体可以将感染了EB病毒的人淋巴细胞用蛋白质、蛋白质表达细胞或其溶解物致敏，使致敏淋巴细胞与来源于人的U266细胞等骨髓瘤细胞进行细胞融合，从融合细胞获得与CAPRIN-1蛋白质的全长或其片段具有免疫反应性的抗体。

人源化抗体是也称为重构(reshaped)人抗体的改变抗体。人源化抗体通过将来源于免疫动物的抗体的互补决定区移植到人抗体的互补性决定区中来构建。作为其一般方法的基因重组方法也是广为人知的技术。具体来说，通过PCR法，由以末端部具有重叠部分的方式制作的数个寡核苷酸，由其合成以将例如小鼠抗体、兔抗体的互补决定区与人抗体的框架区连接的方式设计的DNA序列。将所得的DNA与编码人抗体恒定区的DNA连接，接着整合到表达载体中，将其导入宿主而使其产生，从而得到人源化抗体(参照欧洲专利申请公开第EP239400号、国际公开号WO96/02576)。介由互补决定区连接的人抗体的框架区可以选择使互补性决定区形成良好的抗原结合部位的框架区。根据需要，也可以替换抗体的可变区中的框架区的氨基酸，以使重构人抗体的互补性决定区形成适当的抗原结合部位(Sato K.etal.，Cancer Research 1993，53：851-856)。另外，也可以替换为来源于各种人抗体的框架区(参照WO99/51743)。

抗体通常是至少包含2条重链和2条轻链的杂多聚体糖蛋白质。抗体由2条相同的轻链和2条相同的重链构成。重链在一端具有重链可变区，几个恒定区与其相连。轻链在一端具有轻链可变区，几个恒定区与其相连。可变区显示被称为互补决定区(CDR)的特定可变区，对抗体赋予结合特异性。可变区中相对保守的部分被称为框架区(FR)。完整的重链和轻链的可变区包含分别由3个CDR(CDR1～CDR3)连接而成的4个FR。

此外，来源于人的重链和轻链的恒定区和可变区的序列可以从例如NCBI(美国：GenBank、UniGene等)获得，例如，人IgG1的重链恒定区可以参照登记号J00228的序列，人IgG2的重链恒定区可以参照登记号J00230的序列，人轻链κ恒定区可以参照登记号V00557、X64135、X64133等的序列，人轻链λ恒定区可以参照登记号X64132、X64134等的序列。

嵌合抗体是将来源于不同动物的序列组合而制作的抗体，例如，包含小鼠抗体的重链可变区和轻链可变区以及人抗体的重链可变区和轻链可变区的恒定区的抗体等。嵌合抗体的制作可以使用公知的方法进行，例如可以通过将编码抗体V区的DNA与编码人抗体C区的DNA连接，将其整合到表达载体中并导入宿主，而使其产生，从而得到。

非人动物抗体可以按照公知的方法使致敏抗原免疫动物，作为一般的方法，通过将致敏抗原注射到小鼠等的动物的腹腔内或皮内或皮下来获得。注射致敏抗原时，可以与以CFA(弗氏完全佐剂)为代表的各种佐剂适量混合而对动物多次施与。免疫动物，确认了血清中含有抗CAPRIN-1抗体之后获得血清，如前所述，通过硫酸铵沉淀、蛋白A、蛋白G、DEAE离子交换柱、偶联了CAPRIN-1蛋白质或部分肽的亲和柱等而获得。另外，在从非人动物获得单克隆抗体时，可以通过从免疫后的动物采集免疫细胞，与骨髓瘤细胞进行细胞融合来获得。所述免疫细胞与骨髓瘤细胞的细胞融合可以依照公知的方法进行(参照Kohler,G.andMilstein,C.Methods Enzymol.(1981)73,3-46)。

本发明中使用的抗体也可以作为从杂交瘤克隆抗体基因，整合到适当的载体中，将其导入宿主，使用基因重组技术使其产生的基因重组型抗体获得(参照Carl,A.K.Borrebaeck,James,W.Larrick,THERAPEUTIC MONOCLONAL ANTIBODIES,Published inthe United Kingdom by MACMILLAN PUBLISHERS LTD,1990)。

本发明中使用的抗CAPRIN-1抗体可以是将可变区(例如FR)、恒定区中的氨基酸替换成其他氨基酸而得的抗体。氨基酸替换为1或多个、例如小于15个、小于10个、8个以下、6个以下、5个以下、4个以下、3个以下、或2个以下的氨基酸、优选为1～9个氨基酸的替换，替换后的抗体应当是与未替换抗体相比，对抗原特异地结合的性质、与抗原的结合亲和性为同等或其以上，对人应用时不发生排斥反应的抗体。氨基酸替换期望是保守的氨基酸替换，这是电荷、侧链、极性、芳香族性等性质类似的氨基酸间的替换。性质类似的氨基酸例如可以分类为碱性氨基酸(精氨酸、赖氨酸、组氨酸)、酸性氨基酸(天冬氨酸、谷氨酸)、无电荷极性氨基酸(甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、半胱氨酸、酪氨酸)、无极性氨基酸(亮氨酸、异亮氨酸、丙氨酸、缬氨酸、脯氨酸、苯丙氨酸、色氨酸、蛋氨酸)、支链氨基酸(苏氨酸、缬氨酸、异亮氨酸)、芳香族氨基酸(苯丙氨酸、酪氨酸、色氨酸、组氨酸)等。

本发明中使用的抗CAPRIN-1抗体与癌细胞表面上的CAPRIN-1蛋白质的结合亲和性越高，则越能期待更强的抗肿瘤效果。期望结合常数(亲和常数)Ka(kon/koff)优选为至少10⁷M^-1、至少10⁸M^-1、至少5×10⁸M^-1、至少10⁹M^-1、至少5×10⁹M^-1、至少10¹⁰M^-1、至少5×10¹⁰M^-1、至少10¹¹M^-1、至少5×10¹¹M^-1、至少10¹²M^-1、或至少10¹³M^-1。

本发明中使用的抗CAPRIN-1抗体可以被化学修饰，作为这样的抗体修饰物，可列举例如，与聚乙二醇(PEG)、抗肿瘤性化合物(例如，下述例示的抗肿瘤剂)等各种分子结合的抗体。在本发明的抗体修饰物中，不限定结合的物质。为了得到这样的抗体修饰物，可以通过对所得的抗体实施化学修饰来得到。这些方法在本领域已经被确立。本发明中使用的抗CAPRIN-1抗体通过将抗体的重链恒定区的氨基酸替换1个、2个或数个，或除去与结合于重链恒定区的N-糖苷键糖链中的N-乙酰葡糖胺结合的岩藻糖，能够提高抗CAPRIN-1抗体对效应细胞的结合力。上述可以是单独的氨基酸替换，另外，也可以是与结合了岩藻糖的抗体的组合物。

将重链恒定区的氨基酸替换了1个、2个或数个的抗体可以参照例如WO2004/063351、WO2011/120135、美国专利8388955、WO2011/005481、美国专利6737056、WO2005/063351来制作。

除去了重链恒定区中的N-糖苷键糖链中的N-乙酰葡糖胺所附带的岩藻糖的抗体或产生该抗体的细胞，可以参照美国专利6602684号、欧洲专利1914244、美国专利7579170来制作。除去了与结合于重链恒定区的N-糖苷键糖链中的N-乙酰葡糖胺结合的岩藻糖的抗体和结合了岩藻糖的抗体的组合物或产生该组合物的细胞，可以参照例如美国专利8642292号来制作。

本发明中使用的抗CAPRIN-1多克隆抗体、抗CAPRIN-1单克隆抗体、抗体的制作方法、纯化方法以及用于免疫的CAPRIN-1蛋白质或其部分多肽的制作方法可以参照WO2010/016526、WO2011/096517、WO2011/096528、WO2011/096519、WO2011/096533、WO2011/096534、WO2011/096535、WO2013/018886、WO2013/018894、WO2013/018892、WO2013/018891、WO2013/018889、WO2013/018883、WO2013/125636、WO2013/125654、WO2013/125630、WO2013/125640、WO2013/147169、WO2013/147176以及WO2015/020212获得。

作为本发明中的抗CAPRIN-1抗体的具体例，可列举前述的WO2010/016526、WO2011/096517、WO2011/096528、WO2011/096519、WO2011/096533、WO2011/096534、WO2011/096535、WO2013/018886、WO2013/018894、WO2013/018892、WO2013/018891、WO2013/018889、WO2013/018883、WO2013/125636、WO2013/125654、WO2013/125630、WO2013/125640、WO2013/147169、WO2013/147176以及WO2015/020212中记载的抗CAPRIN-1抗体，但作为优选的抗CAPRIN-1抗体可列举以下抗体。

与具有序列号2或序列号4所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上、更进一步优选为99％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。

与具有序列号31所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号36、37和38的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号40、41和42的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段；或者含有包含序列号140、141和142的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号143、144和145的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段；或者含有包含序列号164、165和166的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号167、168和169的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号39的氨基酸序列、且轻链可变区包含序列号43的氨基酸序列的抗体或其片段；或者重链可变区包含序列号70的氨基酸序列、且轻链可变区包含序列号71的氨基酸序列的抗体或其片段；或者重链可变区包含序列号78的氨基酸序列、且轻链可变区包含序列号79的氨基酸序列的抗体或其片段。

与具有序列号33所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号60、61和62的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号64、65和66的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号63的氨基酸序列、且轻链可变区包含序列号67的氨基酸序列的抗体或其片段。

与具有序列号32所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号52、53和54的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号56、57和58的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号55的氨基酸序列、且轻链可变区包含序列号59的氨基酸序列的抗体或其片段。

与具有序列号34所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号170、171和172的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号173、174和175的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段；或者含有包含序列号176、177和178的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号179、180和181的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号80的氨基酸序列、且轻链可变区包含序列号81的氨基酸序列的抗体或其片段；或者重链可变区包含序列号82的氨基酸序列、且轻链可变区包含序列号83的氨基酸序列的抗体或其片段。

与具有序列号35所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号182、183和184的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号185、186和187的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段；或者含有包含序列号188、189和190的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号191、192和193的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号84的氨基酸序列、且轻链可变区包含序列号85的氨基酸序列的抗体或其片段；或者重链可变区包含序列号86的氨基酸序列、且轻链可变区包含序列号87的氨基酸序列的抗体或其片段。

含有包含序列号44、45和46的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号48、49和50的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。优选为重链可变区包含序列号47的氨基酸序列、且轻链可变区包含序列号51的氨基酸序列的抗体或其片段。

与具有序列号296所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号146、147和148的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号149、150和151的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号72的氨基酸序列、且轻链可变区包含序列号73的氨基酸序列的抗体或其片段。

与具有序列号297所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号272、273和274的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号275、276和277的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号114的氨基酸序列、且轻链可变区包含序列号115的氨基酸序列的抗体或其片段。

与具有序列号298所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号290、291和292的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号293、294和295的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号120的氨基酸序列、且轻链可变区包含序列号121的氨基酸序列的抗体或其片段。

与具有序列号299所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号301、302和303的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号305、306和307的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号300的氨基酸序列、且轻链可变区包含序列号304的氨基酸序列的抗体或其片段。

与具有序列号308所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号134、135和136的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号137、138和139的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号68的氨基酸序列、且轻链可变区包含序列号69的氨基酸序列的抗体或其片段。

与具有序列号309所示的氨基酸序列或相对于该氨基酸序列的序列同一性为80％以上(优选为85％以上、更优选为90％以上、进一步优选为95％以上)的氨基酸序列的CAPRIN-1蛋白质的部分多肽具有免疫反应性的抗体或其片段。优选为含有包含序列号134、135和136的互补决定区(分别为CDR1、CDR2和CDR3)的重链可变区、和包含序列号137、138和139的互补决定区(分别为CDR1、CDR2和CDR3)的轻链可变区，且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段。更优选为重链可变区包含序列号68的氨基酸序列、且轻链可变区包含序列号69的氨基酸序列的抗体或其片段。

另外，还优选使用以下的抗CAPRIN-1抗体。

重链可变区包含序列号68的氨基酸序列、且轻链可变区包含序列号69的氨基酸序列的抗体或其片段。

重链可变区包含序列号70的氨基酸序列、且轻链可变区包含序列号71的氨基酸序列的抗体或其片段。

重链可变区包含序列号72的氨基酸序列、且轻链可变区包含序列号73的氨基酸序列的抗体或其片段。

重链可变区包含序列号74的氨基酸序列、且轻链可变区包含序列号75的氨基酸序列的抗体或其片段。

重链可变区包含序列号76的氨基酸序列、且轻链可变区包含序列号77的氨基酸序列的抗体或其片段。

重链可变区包含序列号78的氨基酸序列、且轻链可变区包含序列号79的氨基酸序列的抗体或其片段。

重链可变区包含序列号80的氨基酸序列、且轻链可变区包含序列号81的氨基酸序列的抗体或其片段。

重链可变区包含序列号82的氨基酸序列、且轻链可变区包含序列号83的氨基酸序列的抗体或其片段。

重链可变区包含序列号84的氨基酸序列、且轻链可变区包含序列号85的氨基酸序列的抗体或其片段。

重链可变区包含序列号86的氨基酸序列、且轻链可变区包含序列号87的氨基酸序列的抗体或其片段。

重链可变区包含序列号88的氨基酸序列、且轻链可变区包含序列号89的氨基酸序列的抗体或其片段。

重链可变区包含序列号90的氨基酸序列、且轻链可变区包含序列号91的氨基酸序列的抗体或其片段。

重链可变区包含序列号92的氨基酸序列、且轻链可变区包含序列号93的氨基酸序列的抗体或其片段。

重链可变区包含序列号94的氨基酸序列、且轻链可变区包含序列号95的氨基酸序列的抗体或其片段。

重链可变区包含序列号96的氨基酸序列、且轻链可变区包含序列号97的氨基酸序列的抗体或其片段。

重链可变区包含序列号98的氨基酸序列、且轻链可变区包含序列号99的氨基酸序列的抗体或其片段。

重链可变区包含序列号100的氨基酸序列、且轻链可变区包含序列号101的氨基酸序列的抗体或其片段。

重链可变区包含序列号102的氨基酸序列、且轻链可变区包含序列号103的氨基酸序列的抗体或其片段。

重链可变区包含序列号104的氨基酸序列、且轻链可变区包含序列号105的氨基酸序列的抗体或其片段。

重链可变区包含序列号106的氨基酸序列、且轻链可变区包含序列号107的氨基酸序列的抗体或其片段。

重链可变区包含序列号108的氨基酸序列、且轻链可变区包含序列号109的氨基酸序列的抗体或其片段。

重链可变区包含序列号110的氨基酸序列、且轻链可变区包含序列号111的氨基酸序列的抗体或其片段。

重链可变区包含序列号112的氨基酸序列、且轻链可变区包含序列号113的氨基酸序列的抗体或其片段。

重链可变区包含序列号114的氨基酸序列、且轻链可变区包含序列号115的氨基酸序列的抗体或其片段。

重链可变区包含序列号116的氨基酸序列、且轻链可变区包含序列号117的氨基酸序列的抗体或其片段。

重链可变区包含序列号118的氨基酸序列、且轻链可变区包含序列号119的氨基酸序列的抗体或其片段。

重链可变区包含序列号120的氨基酸序列、且轻链可变区包含序列号121的氨基酸序列的抗体或其片段。

重链可变区包含序列号122的氨基酸序列、且轻链可变区包含序列号123的氨基酸序列的抗体或其片段。

重链可变区包含序列号124的氨基酸序列、且轻链可变区包含序列号125的氨基酸序列的抗体或其片段。

重链可变区包含序列号126的氨基酸序列、且轻链可变区包含序列号127的氨基酸序列的抗体或其片段。

重链可变区包含序列号128的氨基酸序列、且轻链可变区包含序列号129的氨基酸序列的抗体或其片段。

重链可变区包含序列号130的氨基酸序列、且轻链可变区包含序列号131的氨基酸序列的抗体或其片段。

重链可变区包含序列号132的氨基酸序列、且轻链可变区包含序列号133的氨基酸序列的抗体或其片段。

重链可变区包含序列号300的氨基酸序列、且轻链可变区包含序列号304的氨基酸序列的抗体或其片段。

在后述的实施例中，通过将针对CAPRIN-1蛋白质的全长、在癌细胞的细胞膜表面表达的区域的部分多肽的上述多克隆抗体或单克隆抗体与拓扑异构酶I抑制剂组合，在荷癌生物体内确认了其强的抗肿瘤效果。

＜拓扑异构酶I抑制剂＞

拓扑异构酶I抑制剂选择性地结合与DNA形成复合体的I型拓扑异构酶而使其结构稳定化，引起DNA螺旋结构的解旋阻碍和DNA的片段化，细胞分裂停止而诱导细胞死亡。为了将拓扑异构酶I抑制剂施与生物体，可以包含适当的等渗剂或pH或适当的剂型。作为拓扑异构酶I抑制剂的具体例为拓扑替康(Nogitecan)或伊立替康。拓扑异构酶I抑制剂可以通过本领域技术人员公知的手法进行化学合成而获得。

＜拓扑替康＞

拓扑替康是作为由喜树的树皮提取出的天然物的喜树碱的半合成衍生物，是用CAS号123948-87-8(水合物为119413-54-6)表示的分子量约421.4的I型拓扑异构酶抑制剂。此外，本说明书中仅称为“拓扑替康”的情况下，只要不特别说明，就包含拓扑替康盐酸盐和拓扑替康盐酸盐水合物。拓扑替康盐酸盐的IUPAC名为(S)-10-[(二甲基氨基)甲基]-4-乙基-4,9-二羟基-1H-吡喃并[3’,4’:6,7]中氮茚并[1,2-b]-喹啉-3,14(4H,12H)-二酮单盐酸盐，作为其他的表述，用Nogitecan hydrochloride(Nogitecan盐酸盐)表示。作为以拓扑替康盐酸盐为有效成分的药品，“Hycamtin(注册商标)”、“Potactasol(注册商标)”上市，在本发明中利用拓扑替康时可以适当利用这些药品。

＜伊立替康＞

本发明所涉及的伊立替康是用CAS号100286-90-6(水合物为136572-09-3)表示的分子量约586.678的I型拓扑异构酶抑制剂。此外，本说明书中仅称为“伊立替康”的情况下，只要不特别说明，就包含伊立替康盐酸盐和伊立替康盐酸盐水合物。作为以伊立替康盐酸盐为有效成分的药品，“Campto(注册商标)”、“Topotecin”(注册商标)、“Onivyde(注册商标)”等上市，在本发明中利用伊立替康时可以适当利用这些药品。

＜其他抗肿瘤剂＞

作为本发明的药品的有效成分，在不阻碍作为本发明的药品的效果的范围内，除了所述抗CAPRIN-1抗体和任意的拓扑异构酶I抑制剂之外，还可以包含文献等中公知的抗肿瘤剂。作为公知的抗肿瘤剂不特别限制，作为具体例，包含紫杉醇、多柔比星、柔红霉素、环磷酰胺、甲氨蝶呤、5-氟尿嘧啶、噻替哌、白消安、英丙舒凡、哌泊舒凡、benzodopa、卡波醌、meturedopa、uredopa、六甲蜜胺(altretamine)、三亚乙基三聚氰胺、三亚乙基磷酰胺、三亚乙基硫代磷酰胺(triethilenethiophosphoramide)、三羟甲基三聚氰胺(trimethylolomelamine)、布拉他辛、布拉他辛酮、苔藓虫素、海绵多聚乙酰(callystatin)、cryptophycin1、cryptophycin8、海兔毒素(dolastatin)、倍癌霉素(duocarmycin)、艾榴塞洛素(eleutherobin)、水鬼蕉碱(pancratistatin)、匍枝珊瑚醇(sarcodictyin)、spongistatin、苯丁酸氮芥、萘氮芥(chlornaphazine)、氯磷酰胺(cholophosphamide)、雌莫司汀、异环磷酰胺、甲氮芥、盐酸甲氧氮芥、美法仑、新氮芥(novembichin)、苯芥胆甾醇(phenesterine)、泼尼莫司汀(prednimustine)、曲磷胺(trofosfamide)、乌拉莫司汀、卡莫司汀、氯乙链脲菌素(chlorozotocin)、福莫司汀(fotemustine)、洛莫司汀、尼莫司汀、雷莫司汀、卡奇霉素(calicheamicin)、达内霉素(dynemicin)、氯屈膦酸、埃斯培拉霉素(esperamicin)、阿克拉霉素、放线菌素、安曲霉素(authramycin)、重氮丝氨酸、博来霉素、放线菌素C(cactinomycin)、卡柔比星(carabicin)、洋红霉素、嗜癌菌素(carzinophilin)、色霉素、更生霉素、detorbicin、6-重氮基-5-氧代-L-正亮氨酸、阿霉素(adriamycin)、表柔比星、依索比星、伊达比星、麻西罗霉素(marcellomycin)、丝裂霉素C、霉酚酸(mycophenolic acid)、诺加霉素(nogalamycin)、橄榄霉素(olivomycins)、培洛霉素、甲基丝裂霉素(potfiromycin)、嘌呤霉素、三铁阿霉素(quelamycin)、罗多比星(rodorubicin)、链黑菌素、链佐星、杀结核菌素(tubercidin)、乌苯美司、净司他丁(zinostatin)、佐柔比星(zorubicin)、二甲叶酸(denopterin)、蝶罗呤(pteropterin)、三甲曲沙(trimetrexate)、氟达拉滨(fludarabine)、6-巯嘌呤、硫咪嘌呤、硫鸟嘌呤、安西他滨、阿扎胞苷(azacitidine)、6-氮杂尿苷(azauridine)、卡莫氟、阿糖胞苷、二脱氧尿苷、去氧氟尿苷、依诺他滨(enocitabine)、氟尿苷(floxuridine)；雄激素类、例如卡普睾酮(calusterone)、屈他雄酮丙酸酯、环硫雄醇、美雄烷、睾内酮(testolactone)、氨鲁米特、米托坦、曲洛司坦、亚叶酸(frolinic acid)、醋葡醛内酯、醛磷酰胺糖苷、氨基乙酰丙酸、恩尿嘧啶(eniluracil)、安吖啶(amsacrine)、阿莫司汀(bestrabucil)、比生群(bisantrene)、依达曲沙(edatraxate)、defofamine、秋水仙胺(demecolcine)、地吖醌(diaziquone)、依氟鸟氨酸(elfornithine)、依利醋铵(elliptinium)、埃博霉素(epothilone)、依托格鲁(etoglucid)、香茹多糖、氯尼达明(lonidamine)、美登素(maytansine)、安丝菌素(ansamitocine)、米托胍腙(mitoguazone)、米托蒽醌、mopidanmol、二胺硝吖啶nitraerine、喷司他丁、蛋氨氮芥(phenamet)、吡柔比星、洛索蒽醌(losoxantrone)、足叶草酸(podophyllinic acid)、2-乙基酰肼、甲苄肼、雷佐生(razoxane)、根瘤菌素(rhizoxin)、西佐喃、锗螺胺(spirogermanium)、细交链孢菌酮酸(tenuazonic acid)、三乙撑亚胺苯醌(triaziquone)、杆孢菌素(roridine)A、蛇形菌素(anguidine)、聚氨酯、长春地辛、达卡巴嗪、甘露醇氮芥(mannomustine)、二溴甘露醇、二溴卫矛醇(mitolactol)、哌泊溴烷(pipobroman)、gacytosine、多西他赛、瘤可宁、吉西他滨(gemcitabine)、6-硫鸟嘌呤、巯嘌呤、顺铂、奥沙利铂、卡波铂、长春碱、足叶乙甙、异环磷酰胺、米托葸醌、长春新碱、长春瑞滨、诺消灵(novantrone)、替尼泊苷、依达曲沙(edatrexate)、道诺霉素、氨蝶呤、希罗达(xeloda)、伊班膦酸盐(ibandronate)、二氟甲基鸟氨酸(DMFO)、视黄酸、卡培他滨(capecitabine)、以及它们的药学上可接受的盐或衍生物。

＜本发明的抗肿瘤效果＞

本发明的抗CAPRIN-1抗体与拓扑异构酶I抑制剂的组合在体内(in vivo)具有细胞毒活性。由此本发明的抗肿瘤效果可以通过调查对癌的细胞毒活性而获知。细胞毒活性可以通过对具有癌的生物体施与抗CAPRIN-1和拓扑异构酶I抑制剂，测量施与后的肿瘤的大小，经时地调查癌的大小来评价。另外，本发明的抗肿瘤效果也可以通过调查生存率来评价。另外，也可以通过调查细胞因子或趋化因子的产生能来评价。本发明中由抗CAPRIN-1抗体与拓扑异构酶I抑制剂的组合产生的抗肿瘤效果进一步可以通过调查癌的预防、转移的预防或复发的预防来获知。

本发明中使用的抗CAPRIN-1抗体与癌细胞表面上的CAPRIN-1蛋白质的结合亲和性越高，越可以期待更强的抗肿瘤效果。期望结合常数(亲和常数)Ka(kon/koff)优选为至少10⁷M^-1、至少10⁸M^-1、至少5×10⁸M^-1、至少10⁹M^-1、至少5×10⁹M^-1、至少10¹⁰M^-1、至少5×10¹⁰M^-1、至少10¹¹M^-1、至少5×10¹¹M^-1、至少10¹²M^-1、或至少10¹³M^-1。

本发明中使用的抗CAPRIN-1抗体与CAPRIN-1结合的能力可以利用使用了例如ELISA法、蛋白质印迹法、免疫荧光和流式细胞仪法等的结合检验来特定。

本发明中的抗CAPRIN-1抗体与拓扑异构酶I抑制剂的组合向癌生物体内的施与如前所述与单独的抗CAPRIN-1抗体相比抗肿瘤效果增强，其增强率优选为30％以上、更优选为40％以上、进一步优选为50％以上、进一步更优选为55％以上、进一步更优选为60％以上、进一步更优选为65％以上、最优选为70％以上。本发明中的抗CAPRIN-1抗体与拓扑异构酶I抑制剂的组合施与相对于抗CAPRIN-1抗体单独施与的抗肿瘤效果的增强率,可以通过分别将有效量在相同条件下施与荷癌小鼠，比较施与开始后第7天以后的肿瘤体积来计算。

＜用于癌的治疗和/或预防的药品＞

本发明的药品以癌的治疗和/或预防为目的。成为本发明的药品的靶标的癌，只要是表达CAPRIN-1蛋白质的癌(细胞)就没有特别限定。

本说明书中使用的“治疗”是指基于前述的抗肿瘤效果的癌的治疗。另外，本说明书中使用的“预防”不仅指癌的发生的预防，还指癌的转移或复发的预防。

本说明书中使用的“肿瘤”和“癌”这样的术语是指恶性新生物，可以互换使用。

作为在本发明中成为对象的癌，只要是在细胞膜表面上表达CAPRIN-1蛋白质的癌就可以是任何癌。优选为卵巢癌、胆管癌、乳癌、肾癌、胰癌、大肠癌、黑素瘤(包括术后的黑素瘤)、肺癌(包括非小细胞肺癌、小细胞肺癌)、肾细胞癌、霍奇金淋巴瘤、头颈癌、胃癌、间皮癌(包括恶性胸膜间皮瘤)、结肠/直肠癌(例如具有MSI-high的结肠/直肠癌)、食道癌、食管胃结合部癌、肝细胞癌、胶质母细胞瘤、尿路上皮癌、膀胱癌、子宫癌(包括子宫颈癌、子宫体癌)、原发性中枢神经系统淋巴瘤、原发性睾丸淋巴瘤、胆道癌、脑肿瘤、前列腺癌、白血病、淋巴瘤、肝癌、肉瘤、纤维肉瘤、肥大细胞瘤、肾上腺皮质癌、尤因肉瘤、多发性骨髓瘤、睾丸癌、甲状腺癌、基底细胞癌、佩吉特病或皮肤癌。另外，上述这些癌可以是原发癌、转移性癌、转移了的癌或复发了的癌、术后的癌、或不能切除的癌。此外，黑素瘤经常与恶性黑素瘤或恶性黑色素瘤(malignant melanoma)同义地使用。

此外，作为在本发明中成为对象的癌，可列举对已知的治疗法具有耐性的癌。具有耐性的癌可以是具有任何治疗历史的患者来源的癌，不特别限定，例如，对于有利用铂类制剂或5-FU的治疗史的患者来源的癌，是施与后具有耐性的癌、转移了的癌或复发了的癌。

所述癌更具体地包含例如，鲍温病、黑色素瘤、鳞状细胞癌、乳房外佩吉特病、蕈样真菌病、Sezary综合征、皮肤T/NK细胞淋巴瘤、仅皮肤有病变的T细胞白血病/淋巴瘤、皮肤B细胞淋巴瘤(惰性组)、皮肤T细胞淋巴乳腺癌、复合型乳腺癌、乳腺恶性混合肿瘤、乳管内乳头状腺癌、肺腺癌、鳞状细胞癌、小细胞癌、大细胞癌、作为神经上皮组织性肿瘤的神经胶质瘤、成胶质细胞瘤、成神经细胞瘤、室管膜瘤、神经元肿瘤、胎儿型的神经外胚层肿瘤、神经鞘瘤、纤维神经瘤、脑脊膜瘤、慢性淋巴细胞白血病、淋巴瘤、消化道淋巴瘤、消化器官淋巴瘤、小～中细胞型淋巴瘤、盲肠癌、升结肠癌、降结肠癌、横结肠癌、乙状结肠癌、直肠癌、卵巢上皮癌、胚细胞瘤、间质细胞瘤、胰管癌、浸润性胰管癌、胰癌的腺癌、腺泡细胞癌、腺扁平上皮癌、巨细胞瘤、胰管内乳头粘液性肿瘤、粘液性囊腺癌、胰母细胞瘤、胰头细胞瘤、Frants肿瘤、浆液性囊腺癌、固体乳头状癌、胃泌素瘤、胰高血糖素瘤、胰岛素瘤、多发性内分泌腺瘤综合症1(Wermer综合症)、非功能性胰岛细胞瘤、生长激素抑制素瘤、VIP产生瘤、子宫颈癌、子宫体癌、纤维肉瘤、骨·关节肉瘤、尤文肉瘤、肾母细胞瘤(Wilms’tumor)、肝母细胞瘤、软组织肉瘤、急性白血病、慢性白血病、脊髓肿瘤、软组织恶性肿瘤、畸胎癌肿瘤、头颈癌包括下咽癌、中咽癌、舌癌、上咽癌、口腔癌、口唇癌、鼻窦癌、喉癌、肾盂输尿管癌、膀胱癌、尿道癌、精巢肿瘤、恶性胸膜间皮瘤、恶性骨肿瘤、子宫体癌(术后的化疗、转移复发时化疗)、儿童恶性实体肿瘤(横纹肌肉瘤、成神经细胞瘤、肝母细胞瘤、成神经细胞瘤、肾母细胞瘤、视网膜母细胞瘤、中枢神经系统胚胎细胞肿瘤、尤文肉瘤家族肿瘤)、浆液性肿瘤(包括浆液性边界恶性肿瘤(SBT))、粘液性肿瘤、表层上皮性边界恶性肿瘤、间质性边界恶性肿瘤等，但不限于这些。另外包含以上述癌为原发的能够触诊的癌、存在于皮下的癌、存在于皮内的癌、浅表性的癌、存在于真皮的癌或存在于非实体器官的癌、渐进性的癌。另外包含以上述癌为原发、通过转移或复发而能够触诊的癌、存在于皮下的癌、存在于皮内的癌、浅表性的癌、存在于真皮的癌或存在于非实体器官的癌。

另外，成为对象的优选的受试者(患者)是哺乳动物，例如是包含灵长类、宠物、家畜类、竞技用动物等的哺乳动物，特别优选是人、犬和猫。

本发明的药品可以利用本领域技术人员公知的方法进行制剂化。本发明的药品可以以例如在水或除水以外的药学上容许的液体中的无菌性溶液、或悬浮液剂的注射剂的形式非经口地使用。在本发明的药品中，也可以对每种制剂或药物组合物将其有效成分(抗CAPRIN-1抗体和拓扑异构酶I抑制剂)与例如与药理学上容许的载体或介质、具体来说为灭菌水、生理盐水、等渗液、缓冲剂(缓冲液等)、植物油、油性油、氧化防止剂、助溶剂、乳化剂、悬浮剂、表面活性剂、稳定剂、芳香剂、赋形剂、结合剂等适当组合，优选可以通过以一般认为的制药实施所要求的单位用量方式与它们混和来进行制剂化。这些制剂中的有效成分量，是可得到所指示的范围的适当的用量那样的量。

用于注射的无菌组合物可以使用注射用蒸馏水这样的媒介物，按照通常的制剂实施来处方。作为注射用的水溶液，可以列举例如，生理盐水、含有葡萄糖和/或其他辅助剂、例如D-山梨糖醇、D-甘露糖、D-甘露醇、氯化钠的等渗液，也可以与适当的溶解助剂、例如醇、具体为乙醇、多元醇例如丙二醇、聚乙二醇、非离子性表面活性剂例如聚山梨醇酯80(TM)、HCO-60联合使用。作为油性液可以列举芝麻油、大豆油，其也可以与作为溶解助剂的苯甲酸苄酯、苄醇联合使用。另外，还可以与缓冲剂例如磷酸盐缓冲液、乙酸钠缓冲液、镇痛剂例如盐酸普鲁卡因、稳定剂例如苄醇、苯酚、抗氧化剂配合。制备的注射液通常填充到适当的安瓿中即可。

施与为经口或非经口，优选非经口施与，具体来说，可以列举注射剂型、经鼻施与剂型、经肺施与剂型、经皮施与型等。作为注射剂型的例子，可以通过例如静脉内注射、肌肉内注射、腹腔内注射、皮下注射、肿瘤内注射等全身或局部性地施与。作为经皮施与型的例子，例如为被称为涂布剂或外用药的剂型。作为外用药，可列举固体制剂、液剂、喷雾剂、软膏剂、乳膏剂、或凝胶剂。

另外，可以根据患者的年龄、体重、性别、症状等选择适当的施与方法。作为含有抗CAPRIN-1抗体和拓扑异构酶I抑制剂的至少一者的药物组合物的施与量，以各有效成分的量记，例如，可以在一次每1kg体重为0.0001mg至1000mg的范围中选择。或者例如可以在每个患者0.001～100000mg/个体的范围、或例如患者的每1kg体重1mg至30mg中选择施与量，但未必限于这些数值。施与量、施与方法根据患者的体重、年龄、性别、症状等不同而变化，只要是本领域技术人员就可以适当选择。

＜施与方法＞

利用本发明的用于癌的治疗和/或预防的药品的癌的治疗和/或预防，除了作为前述的药品施与之外，包含各种形式。例如，本发明的药品的各有效成分可以同时或按顺序逐个施与。作为具体例，可以以到约3周的时间间隔内、即在从施与第1有效成分之后开始到约3周施与第2有效成分。此时，可以接着外科处置之后而实施，也可以在第1药剂与第2药剂的施与之间实施外科处置。另外，可以将本发明的癌的治疗和/或预防剂按照多个施与循环进行施与。例如，在实施本发明的癌的治疗和/或预防剂的各有效成分的同时施与的情况下，将包含双方的有效成分的药物组合物以约2天至约3周为1循环进行施与。然后，按照负责的医师的判断，根据需要可以将该治疗循环重复。同样地在计划按照顺序的处方时，分别将各个药剂的施与期间调整为相同期间。循环之间的间隔可以在0～2个月变化。本发明的癌的治疗和/或预防剂的各有效成分的施与量可以与药物组合物中的各有效成分的施与量同样地设定。

＜制药试剂盒＞

本发明的癌的治疗和/或预防用的药品可以是制药试剂盒的形态。制药试剂盒是指在治疗和/或预防癌的方法中，用于将有效成分以单个的药物组合物的形态使用的包，该包包含用于施与各有效成分的使用说明书。制药试剂盒中包含的癌的治疗和/或预防用的上述药物组合物的各有效成分，可以是以能够将各有效成分一起或分别施与的方式分别如上所述制剂化而成的药物组合物的形态。另外，制药试剂盒为了能够将各有效成分按照上述施与方法施与，包含对于1次或多次的用量为充分的量的有效成分。

基于上面具体说明的内容，本发明进一步提供包括将本发明的上述药品施与怀疑具有癌的受检者的用于癌的治疗和/或预防的方法。另外，在其实施方式中，上述药品所含的抗体或其片段和抗肿瘤剂可以同时或分别施与上述受检者。

实施例

以下，基于实施例具体地说明本发明，但本发明的范围不受这些具体例限制。

(实施例1)抗CAPRIN-1抗体的制作

本发明中使用与CAPRIN-1蛋白质具有免疫反应性的抗CAPRIN-1抗体，使用如下制作的抗体。

(多克隆抗体)

将按照WO2010/016526的实施例3制作的人CAPRIN-1重组蛋白质1mg与等体积的氟氏不完全佐剂(IFA)溶液混合，将其每隔2周在兔的皮下进行施与，共施与4次。然后采集血液，获得包含多克隆抗体的抗血清。进一步将该抗血清使用蛋白G载体(GEヘルスケアバイオサイエンス社制)纯化，替换为PBS(-)，得到针对CAPRIN-1蛋白质的多克隆抗体(抗CAPRIN-1多克隆抗体#1)。

(单克隆抗体)

将按照WO2010/016526的实施例3制作的人CAPRIN-1重组蛋白质100μg与等量的MPL+TDM佐剂(シグマ社制)混合，将此作为每1只小鼠的抗原溶液。将抗原溶液施与到6周龄的Balb/c小鼠(日本SLC社制)的腹腔内后，每1周进一步进行施与3次和24次，从而完成免疫。从最后的免疫起3天后摘出的各自的脾脏用灭菌后的2枚载玻片夹持磨碎，将使用PBS(-)(日水社制)洗涤、以1500rpm离心10分钟并除去上清的操作重复3次，获得脾脏细胞。将所得的脾脏细胞与小鼠骨髓瘤细胞SP2/0(从ATCC购入)以10:1的比率混和，在其中加入将加温至37℃的包含10％FBS的RPMI1640培养基200μL和PEG1500(ベーリンガー社制)800μL混合而制备的PEG溶液，并静置5分钟，从而进行细胞融合。以1700转/分钟离心5分钟，除去上清，然后用加入了2％当量的Gibco社制的HAT溶液的含15％FBS的RPMI1640培养基(HAT选择培养基)150ml悬浮细胞，以96孔板(ヌンク社制)的每1孔各100μl接种于15块板中。以7天、37℃、5％CO₂的条件培养，从而得到脾脏细胞与骨髓瘤细胞融合而成的杂交瘤。以由制作的杂交瘤所产生的抗体的对CAPRIN-1蛋白质的结合亲和性为指标筛选杂交瘤。将CAPRIN-1蛋白质溶液1μg/ml在96孔板每1孔中添加100μL，在4℃静置18小时。将各孔用PBS-T洗涤3次后，在每1孔中添加0.5％牛血清白蛋白(BSA)溶液(シグマ社制)400μL，在室温静置3小时。除去溶液，对每1孔用400μL的PBS-T洗涤孔3次后，在每1孔中添加上述所得的杂交瘤的各培养上清100μL，在室温静置2小时。用PBS-T洗涤各孔3次后，在每1孔中添加用PBS稀释至5000倍的HRP标记抗小鼠IgG(H+L)抗体(インビトロジェン社制)100μL，在室温静置1小时。用PBS-T洗涤孔3次后，在每1孔中添加TMB底物溶液(Thermo社制)100μl，并静置15～30分钟，从而进行显色反应。显色后，在每1孔中添加1N硫酸100μl使反应停止，使用吸光度计测定450nm和595nm的吸光度值。其结果是，筛选出了多个产生吸光度值高的抗体的杂交瘤。将筛选出的杂交瘤以96孔板每1孔0.5个的方式添加到板中进行培养。1周后，观察到在孔中形成单个集落的杂交瘤。将这些孔的细胞进行进一步培养，以由克隆化的杂交瘤产生的抗体对CAPRIN-1蛋白质的结合亲和性为指标筛选杂交瘤。将CAPRIN-1蛋白质溶液1μg/ml在96孔板的每1孔中添加100μL，在4℃静置18小时。用PBS-T洗涤各孔3次后，在每1孔中添加0.5％BSA溶液400μL，并在室温静置3小时。除去溶液，对每1孔用400μL的PBS-T洗涤孔3次后，在每1孔中添加上述所得的杂交瘤的各培养上清100μL，在室温静置2小时。用PBS-T洗涤各孔3次后，在每1孔中添加用PBS稀释至5000倍的HRP标记抗小鼠IgG(H+L)抗体(インビトロジェン社制)100μL并在室温静置1小时。用PBS-T洗涤孔3次后，在每1孔中添加TMB底物溶液(Thermo社制)100μL，并静置15～30分钟，从而进行显色反应。显色后，在每1孔中添加1N硫酸100μL使反应停止，使用吸光度计测定450nm和595nm的吸光度值。其结果是，得到了多个对CAPRIN-1蛋白质显示反应性的小鼠单克隆抗体。

进而通过流式细胞仪法确认对人癌细胞的反应性，所述人癌细胞是已经确认了在细胞膜表面表达CAPRIN-1蛋白质的细胞。作为阴性对照使用不与所述癌细胞显示反应性的小鼠IgG对照抗体。确认的结果是，对所述癌细胞，得到了数个与小鼠IgG对照抗体相比荧光强度强、与在细胞膜表面表达CAPRIN-1的上述癌细胞的细胞膜表面强烈地反应的单克隆抗体。从中作为与CAPRIN-1蛋白质显示反应性的单克隆抗体，筛选出了作为WO2013/125630中记载的针对CAPRIN-1的单克隆抗体的所述(ab)的包含序列号114所示的重链可变区的氨基酸序列、和序列号115所示的轻链可变区的氨基酸序列的抗体。

特定上述筛选出的抗体的重链可变区的CDR1～3，以能够表达框架区包含人抗体的序列的重链可变区的方式设计碱基序列，将其插入到已经插入有人IgG1的重链恒定区的哺乳类表达用载体中。同样地，特定轻链可变区的CDR1～3，以能够表达框架区包含人抗体的序列的轻链可变区的方式设计碱基序列，将其插入到已经插入有人IgG1的轻链恒定区的哺乳类表达用载体中。将上述2种重组表达载体按照常规方法导入哺乳类细胞，获得包含针对CAPRIN-1的人源化单克隆抗体#1(人源化抗体#1)的培养上清。

将所得的包含人源化抗CAPRIN-1单克隆抗体#1的培养上清按照常规方法使用Hitrap Protein A SepharoseFF(GEヘルスケア社制)进行纯化，替换成PBS(-)，用0.22μm的过滤器(ミリポア社制)进行过滤，从而制备。

上述抗CAPRIN-1抗体对CAPRIN-1蛋白质的特异反应性通过将CAPRIN-1蛋白质在平板上固相化使用ELISA法进行检测来确认。

此外，使用上述抗CAPRIN-1抗体通过流式细胞仪法调查与未进行细胞膜的透过处理的癌细胞的反应性，从而如以下实施例所示，确认了CAPRIN-1的一部分在癌细胞的细胞膜表面表达。

通过流式细胞仪法确认了，对作为确认了CAPRIN-1的基因的表达的人癌细胞的乳癌细胞(BT-474)、大肠癌细胞(HT-29)、肺癌细胞(QG56、H1650)、胃癌细胞(NCI-N87)、子宫癌细胞(HEC-1-A)、前列腺癌细胞(22Rv1)、胰脏癌细胞(Panc10.5)、肝脏癌细胞(Hep3B)、卵巢癌细胞(SKOV3)、肾癌细胞(Caki-2)、脑肿瘤细胞(U-87MG)、膀胱癌细胞(T24)、食道癌细胞(OE33)、白血病细胞(OCI-AML5)、淋巴瘤细胞(Ramos)、胆囊癌细胞(TGBC14TKB)、纤维肉瘤细胞(HT-1080)、黑素瘤细胞(G-361)、确认了CAPRIN-1的基因的表达的小鼠肾癌细胞(Renca)、小鼠乳癌细胞(4T1)的任意癌细胞，人源化抗体#1都比对作为阴性对照的癌细胞不显示反应性的人IgG对照抗体以及兔IgG抗体荧光强度强，确认了与CAPRIN-1表达的上述癌细胞的细胞膜表面强烈地反应.

同样地，对于WO2010/016526、WO2011/096517、WO2011/096528、WO2011/096519、WO2011/096533、WO2011/096534、WO2011/096535、WO2013/018886、WO2013/018894、WO2013/018892、WO2013/018891、WO2013/018889、WO2013/018883、WO2013/125636、WO2013/125654、WO2013/125640、WO2013/147169、WO2013/147176以及WO2015/020212中记载的所述(a)～(aa)和(ac)～(al)的各抗CAPRIN-1抗体，也同样地确认了与上述癌细胞膜表面强烈地反应。

(实施例2)由抗CAPRIN-1抗体与拓扑替康的联合使用产生的抗肿瘤效果

对于实施例1中制作的抗CAPRIN-1抗体(抗CAPRIN-1人源化抗体#1)与拓扑替康的联合使用产生的荷癌小鼠生物体内的抗肿瘤效果进行评价。

具体地，使用将表达CAPRIN-1蛋白质的人来源的癌细胞移植到皮下而得的NOD-SCID小鼠，研究本发明中的由抗CAPRIN-1抗体与拓扑替康的联合使用产生的抗肿瘤效果。对每1只小鼠，将2×10⁷个人乳癌细胞BT474与Matrigel(基质胶)(SIGMA)混合而移植到皮下，使其成长到肿瘤为约154～155mm³，从而制作荷癌小鼠。BT474在细胞膜表面表达CAPRIN-1蛋白质，是确认了实施例1中制作的抗CAPRIN-1抗体与位于细胞膜表面的CAPRIN-1的一部分反应的癌细胞。分别对8只上述荷癌小鼠的尾静脉各以10mg/kg施与实施例1中制作的抗CAPRIN-1抗体，一周各施与1次，共计施与4次。对相同的小鼠，每4天各施与低用量(3mg/kg)或高用量(10mg/kg)的拓扑替康1次，共计施与4次。

作为比较对照组，对荷癌小鼠将与上述相同的抗CAPRIN-1抗体以等量一周施与各1次。进而作为比较对照组，对别的荷癌小鼠个体将低用量(3mg/kg)或高用量(10mg/kg)的拓扑替康以同样的施与间隔进行施与。进而将无治疗组的荷癌小鼠作为阴性对照。开始施与后，经时地使用游标卡尺测量荷癌小鼠的癌的大小，肿瘤体积按照常规方法，通过算式：(癌的长径的长度)×(癌的短径的长度)²×0.5对每个个体计算出施与组的平均值。

评价的结果是，在药剂施与结束约2周后(荷癌后第56天)，在以阴性对照的肿瘤体积为100％时，作为比较对象组的实施例1中制作的抗CAPRIN-1人源化抗体#1施与组的肿瘤体积为38％，拓扑替康低用量施与组中为33％，以及拓扑替康高用量施与组中为16％，肿瘤体积比施与开始时增大。另一方面，联合施与了实施例1中制作的人源化抗体#1和拓扑替康低用量的组的肿瘤体积为6％，以及联合施与了实施例1中制作的人源化抗体#1和拓扑替康高用量的组的肿瘤体积为0％(在全部个体中肿瘤退缩)，比施与开始时缩小。

同样地确认了，对于WO2010/016526、WO2011/096517、WO2011/096528、WO2011/096519、WO2011/096533、WO2011/096534、WO2011/096535、WO2013/018886、WO2013/018894、WO2013/018892、WO2013/018891、WO2013/018889、WO2013/018883、WO2013/125636、WO2013/125654、WO2013/125640、WO2013/147169、WO2013/147176以及WO2015/020212记载的所述(a)～(aa)和(ac)～(al)的各抗CAPRIN-1抗体，通过组合拓扑替康而施与，从而与单独施与抗CAPRIN-1抗体的情况、以及单独施与拓扑替康的情况相比，具有显著强的抗肿瘤效果。

本评价的结果显示，通过组合施与抗CAPRIN-1抗体与拓扑替康，与单独施与抗CAPRIN-1抗体的情况、以及单独施与拓扑替康的情况相比，具有显著强的抗肿瘤效果。

(比较例1)由抗CAPRIN-1抗体与其他拓扑异构酶抑制剂的联合使用产生的抗肿瘤效果

接着，对于实施例1中制作的抗CAPRIN-1抗体(抗CAPRIN-1人源化抗体#1)与作为不同于拓扑替康的别的拓扑异构酶抑制剂(拓扑异构酶II抑制剂)的依托泊苷的联合使用产生的荷癌小鼠生物体内的抗肿瘤效果进行评价。

具体地，使用将表达CAPRIN-1蛋白质的人来源的癌细胞移植到皮下而得的NOD-SCID小鼠，研究由抗CAPRIN-1抗体与依托泊苷的联合使用产生的抗肿瘤效果。对每1只小鼠，将2×10⁷个人乳癌细胞BT474与Matrigel(基质胶)(SIGMA)混合而移植到皮下，使其成长到肿瘤为约190～192mm³，从而制作荷癌小鼠。BT474在细胞膜表面表达CAPRIN-1蛋白质，是确认了实施例1中制作的抗CAPRIN-1抗体与位于细胞膜表面的CAPRIN-1的一部分反应的癌细胞。分别对8只上述荷癌小鼠的尾静脉各以10mg/kg施与实施例1中制作的抗CAPRIN-1抗体，一周各施与1次，共计施与4次。对相同的小鼠，每4天各施与低用量(5mg/kg)或高用量(15mg/kg)的依托泊苷1次，共计施与4次。

作为比较对照组，对荷癌小鼠将与上述相同的抗CAPRIN-1抗体以等量一周施与各1次。进而作为比较对照组，对别的荷癌小鼠个体将低用量(5mg/kg)或高用量(15mg/kg)的依托泊苷以同样的施与间隔进行施与。进而将无治疗组的荷癌小鼠作为阴性对照。开始施与后，经时地使用游标卡尺测量荷癌小鼠的癌的大小，肿瘤体积按照常规方法，通过算式：(癌的长径的长度)×(癌的短径的长度)²×0.5对每个个体计算出施与组的平均值。

评价的结果是，在药剂施与结束约2周后(荷癌后第55天)，在以阴性对照的肿瘤体积为100％时，作为比较对象组的实施例1中制作的抗CAPRIN-1人源化抗体#1施与组的肿瘤体积为35％，依托泊苷低用量施与组中为92％，依托泊苷高用量施与组中为79％，联合施与了实施例1中制作的人源化抗体#1和依托泊苷低用量的组的肿瘤体积为18％，以及联合施与了实施例1中制作的人源化抗体#1和依托泊苷高用量的组的肿瘤体积为30％，在任意处置组中肿瘤体积比施与开始时增大。

与实施例2所示的由与拓扑替康的联合使用产生的抗肿瘤效果的评价结果相比，组合针对CAPRIN-1的抗体与依托泊苷而处置的情况下的抗肿瘤效果低，显示了在组合针对CAPRIN-1的抗体与作为拓扑异构酶I抑制剂的拓扑替康而处置的情况下，具有显著强的协同的抗肿瘤效果。

序列表

<110> 东丽株式会社

<120> 东丽株式会社

<130> 21B0436

<160> 309

<170> PatentIn 版本 3.5

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<213> 人(Homo sapiens)

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<222> (190)..(2319)

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cagagggctg ctggctggct aagtccctcc cgctcccggc tctcgcctca ctaggagcgg 60

ctctcggtgc agcgggacag ggcgaagcgg cctgcgccca cggagcgcgc gacactgccc 120

ggaagggacc gccacccttg ccccctcagc tgcccactcg tgatttccag cggcctccgc 180

gcgcgcacg atg ccc tcg gcc acc agc cac agc ggg agc ggc agc aag tcg 231

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser

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tcc gga ccg cca ccg ccg tcg ggt tcc tcc ggg agt gag gcg gcc gcg 279

Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala

15 20 25 30

gga gcc ggg gcc gcc gcg ccg gct tct cag cac ccc gca acc ggc acc 327

Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr

35 40 45

ggc gct gtc cag acc gag gcc atg aag cag att ctc ggg gtg atc gac 375

Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp

50 55 60

aag aaa ctt cgg aac ctg gag aag aaa aag ggt aag ctt gat gat tac 423

Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr

65 70 75

cag gaa cga atg aac aaa ggg gaa agg ctt aat caa gat cag ctg gat 471

Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp

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gcc gtt tct aag tac cag gaa gtc aca aat aat ttg gag ttt gca aaa 519

Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys

95 100 105 110

gaa tta cag agg agt ttc atg gca cta agt caa gat att cag aaa aca 567

Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr

115 120 125

ata aag aag aca gca cgt cgg gag cag ctt atg aga gaa gaa gct gaa 615

Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu

130 135 140

cag aaa cgt tta aaa act gta ctt gag cta cag tat gtt ttg gac aaa 663

Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys

145 150 155

ttg gga gat gat gaa gtg cgg act gac ctg aaa caa ggt ttg aat gga 711

Leu Gly Asp Asp Glu Val Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly

160 165 170

gtg cca ata ttg tcc gaa gag gag ttg tca ttg ttg gat gaa ttc tat 759

Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr

175 180 185 190

aag cta gta gac cct gaa cgg gac atg agc ttg agg ttg aat gaa cag 807

Lys Leu Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln

195 200 205

tat gaa cat gcc tcc att cac ctg tgg gac ctg ctg gaa ggg aag gaa 855

Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu

210 215 220

aaa cct gta tgt gga acc acc tat aaa gtt cta aag gaa att gtt gag 903

Lys Pro Val Cys Gly Thr Thr Tyr Lys Val Leu Lys Glu Ile Val Glu

225 230 235

cgt gtt ttt cag tca aac tac ttt gac agc acc cac aac cac cag aat 951

Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn

240 245 250

ggg ctg tgt gag gaa gaa gag gca gcc tca gca cct gca gtt gaa gac 999

Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Ala Val Glu Asp

255 260 265 270

cag gta cct gaa gct gaa cct gag cca gca gaa gag tac act gag caa 1047

Gln Val Pro Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln

275 280 285

agt gaa gtt gaa tca aca gag tat gta aat aga cag ttc atg gca gaa 1095

Ser Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu

290 295 300

aca cag ttc acc agt ggt gaa aag gag cag gta gat gag tgg aca gtt 1143

Thr Gln Phe Thr Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val

305 310 315

gaa acg gtt gag gtg gta aat tca ctc cag cag caa cct cag gct gca 1191

Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala

320 325 330

tcc cct tca gta cca gag ccc cac tct ttg act cca gtg gct cag gca 1239

Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ala

335 340 345 350

gat ccc ctt gtg aga aga cag cga gta caa gac ctt atg gca caa atg 1287

Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met

355 360 365

cag ggt ccc tat aat ttc ata cag gat tca atg ctg gat ttt gaa aat 1335

Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn

370 375 380

cag aca ctt gat cct gcc att gta tct gca cag cct atg aat cca aca 1383

Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr

385 390 395

caa aac atg gac atg ccc cag ctg gtt tgc cct cca gtt cat tct gaa 1431

Gln Asn Met Asp Met Pro Gln Leu Val Cys Pro Pro Val His Ser Glu

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tct aga ctt gct cag cct aat caa gtt cct gta caa cca gaa gcg aca 1479

Ser Arg Leu Ala Gln Pro Asn Gln Val Pro Val Gln Pro Glu Ala Thr

415 420 425 430

cag gtt cct ttg gta tca tcc aca agt gag ggg tac aca gca tct caa 1527

Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln

435 440 445

ccc ttg tac cag cct tct cat gct aca gag caa cga cca cag aag gaa 1575

Pro Leu Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu

450 455 460

cca att gat cag att cag gca aca atc tct tta aat aca gac cag act 1623

Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr

465 470 475

aca gca tca tca tcc ctt cct gct gcg tct cag cct caa gta ttt cag 1671

Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln

480 485 490

gct ggg aca agc aaa cct tta cat agc agt gga atc aat gta aat gca 1719

Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala

495 500 505 510

gct cca ttc caa tcc atg caa acg gtg ttc aat atg aat gcc cca gtt 1767

Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val

515 520 525

cct cct gtt aat gaa cca gaa act tta aaa cag caa aat cag tac cag 1815

Pro Pro Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln

530 535 540

gcc agt tat aac cag agc ttt tct agt cag cct cac caa gta gaa caa 1863

Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln

545 550 555

aca gag ctt cag caa gaa cag ctt caa aca gtg gtt ggc act tac cat 1911

Thr Glu Leu Gln Gln Glu Gln Leu Gln Thr Val Val Gly Thr Tyr His

560 565 570

ggt tcc cca gac cag tcc cat caa gtg act ggt aac cac cag cag cct 1959

Gly Ser Pro Asp Gln Ser His Gln Val Thr Gly Asn His Gln Gln Pro

575 580 585 590

cct cag cag aac act gga ttt cca cgt agc aat cag ccc tat tac aat 2007

Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr Tyr Asn

595 600 605

agt cgt ggt gtg tct cgt gga ggc tcc cgt ggt gct aga ggc ttg atg 2055

Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met

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aat gga tac cgg ggc cct gcc aat gga ttc aga gga gga tat gat ggt 2103

Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly

625 630 635

tac cgc cct tca ttc tct aac act cca aac agt ggt tat aca cag tct 2151

Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Thr Gln Ser

640 645 650

cag ttc agt gct ccc cgg gat tac tct ggc tat caa cgg gat gga tat 2199

Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr

655 660 665 670

cag cag aat ttc aag cga ggc tct ggg cag agt gga cca cgg gga gcc 2247

Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala

675 680 685

cca cga ggt cgt gga ggg ccc cca aga ccc aac aga ggg atg ccg caa 2295

Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln

690 695 700

atg aac act cag caa gtg aat taa tctgattcac aggattatgt ttaatcgcca 2349

Met Asn Thr Gln Gln Val Asn

705

aaaacacact ggccagtgta ccataatatg ttaccagaag agttattatc tatttgttct 2409

ccctttcagg aaacttattg taaagggact gttttcatcc cataaagaca ggactacaat 2469

tgtcagcttt ctattacctg gatatggaag gaaactattt ttactctgca tgttctgtcc 2529

taagcgtcat cttgagcctt gcacatgata ctcagattcc tcacccttgc ttaggagtaa 2589

aacaatatac tttacagggt gataataatc tccatagtta tttgaagtgg cttgaaaaag 2649

gcaagattga cttttatgac attggataaa atctacaaat cagccctcga gttattcaat 2709

gataactgac aaactaaatt atttccctag aaaggaagat gaaaggagtg gagtgtggtt 2769

tggcagaaca actgcatttc acagcttttc cagttaaatt ggagcactga acgttcagat 2829

gcataccaaa ttatgcatgg gtcctaatca cacatataag gctggctacc agctttgaca 2889

cagcactgtt catctggcca aacaactgtg gttaaaaaca catgtaaaat gctttttaac 2949

agctgatact gtataagaca aagccaagat gcaaaattag gctttgattg gcactttttg 3009

aaaaatatgc aacaaatatg ggatgtaatc cggatggccg cttctgtact taatgtgaaa 3069

tatttagata cctttttgaa cacttaacag tttctttgag acaatgactt ttgtaaggat 3129

tggtactatc tatcattcct tatgacatgt acattgtctg tcactaatcc ttggattttg 3189

ctgtattgtc acctaaattg gtacaggtac tgatgaaaat ctctagtgga taatcataac 3249

actctcggtc acatgttttt ccttcagctt gaaagctttt ttttaaaagg aaaagatacc 3309

aaatgcctgc tgctaccacc cttttcaatt gctatctttt gaaaggcacc agtatgtgtt 3369

ttagattgat ttccctgttt cagggaaatc acggacagta gtttcagttc tgatggtata 3429

agcaaaacaa ataaaacgtt tataaaagtt gtatcttgaa acactggtgt tcaacagcta 3489

gcagcttatg tgattcaccc catgccacgt tagtgtcaca aattttatgg tttatctcca 3549

gcaacatttc tctagtactt gcacttatta tcttttgtct aatttaacct taactgaatt 3609

ctccgtttct cctggaggca tttatattca gtgataattc cttcccttag atgcataggg 3669

agagtctcta aatttgatgg aaatggacac ttgagtagtg acttagcctt atgtactctg 3729

ttggaatttg tgctagcagt ttgagcacta gttctgtgtg cctaggaagt taatgctgct 3789

tattgtctca ttctgacttc atggagaatt aatcccacct ttaagcaaag gctactaagt 3849

taatggtatt ttctgtgcag aaattaaatt ttattttcag catttagccc aggaattctt 3909

ccagtaggtg ctcagctatt taaaaacaaa actattctca aacattcatc attagacaac 3969

tggagttttt gctggttttg taacctacca aaatggatag gctgttgaac attccacatt 4029

caaaagtttt gtagggtggt gggaaatggg ggatcttcaa tgtttatttt aaaataaaat 4089

aaaataagtt cttgactttt ctcatgtgtg gttgtggtac atcatattgg aagggttaac 4149

ctgttacttt ggcaaatgag tatttttttg ctagcacctc cccttgcgtg ctttaaatga 4209

catctgcctg ggatgtacca caaccatatg ttacctgtat cttaggggaa tggataaaat 4269

atttgtggtt tactgggtaa tccctagatg atgtatgctt gcagtcctat ataaaactaa 4329

atttgctatc tgtgtagaaa ataatttcat gacatttaca atcaggactg aagtaagttc 4389

ttcacacagt gacctctgaa tcagtttcag agaagggatg ggggagaaaa tgccttctag 4449

gttttgaact tctatgcatt agtgcagatg ttgtgaatgt gtaaaggtgt tcatagtttg 4509

actgtttcta tgtatgtttt ttcaaagaat tgttcctttt tttgaactat aatttttctt 4569

tttttggtta ttttaccatc acagtttaaa tgtatatctt ttatgtctct actcagacca 4629

tatttttaaa ggggtgcctc attatggggc agagaacttt tcaataagtc tcattaagat 4689

ctgaatcttg gttctaagca ttctgtataa tatgtgattg cttgtcctag ctgcagaagg 4749

ccttttgttt ggtcaaatgc atattttagc agagtttcaa ggaaatgatt gtcacacatg 4809

tcactgtagc ctcttggtgt agcaagctca catacaaaat acttttgtat atgcataata 4869

taaatcatct catgtggata tgaaacttct tttttaaaac ttaaaaaggt agaatgttat 4929

tgattacctt gattagggca gttttatttc cagatcctaa taattcctaa aaaatatgga 4989

aaagtttttt ttcaatcatt gtaccttgat attaaaacaa atatccttta agtatttcta 5049

atcagttagc ttctacagtt cttttgtctc cttttatatg cagctcttac gtgggagact 5109

tttccactta aaggagacat agaatgtgtg cttattctca gaaggttcat taactgaggt 5169

gatgagttaa caactagttg agcagtcagc ttcctaagtg ttttaggaca tttgttcatt 5229

atattttccg tcatataact agaggaagtg gaatgcagat aagtgccgaa ttcaaaccct 5289

tcattttatg tttaagctcc tgaatctgca ttccacttgg gttgttttta agcattctaa 5349

attttagttg attataagtt agatttcaca gaatcagtat tgcccttgat cttgtccttt 5409

ttatggagtt aacggggagg aagacccctc aggaaaacga aagtaaattg ttaaggctca 5469

tcttcatacc tttttccatt ttgaatccta caaaaatact gcaaaagact agtgaatgtt 5529

taaaattaca ctagattaaa taatatgaaa gtc 5562

<210> 2

<211> 709

<212> PRT

<213> 人(Homo sapiens)

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Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala

35 40 45

Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys

50 55 60

Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu

65 70 75 80

Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val

85 90 95

Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu

100 105 110

Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys

115 120 125

Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys

130 135 140

Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly

145 150 155 160

Asp Asp Glu Val Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly Val Pro

165 170 175

Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu

180 185 190

Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu

195 200 205

His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro

210 215 220

Val Cys Gly Thr Thr Tyr Lys Val Leu Lys Glu Ile Val Glu Arg Val

225 230 235 240

Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu

245 250 255

Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Ala Val Glu Asp Gln Val

260 265 270

Pro Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu

275 280 285

Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln

290 295 300

Phe Thr Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr

305 310 315 320

Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro

325 330 335

Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ala Asp Pro

340 345 350

Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly

355 360 365

Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr

370 375 380

Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn

385 390 395 400

Met Asp Met Pro Gln Leu Val Cys Pro Pro Val His Ser Glu Ser Arg

405 410 415

Leu Ala Gln Pro Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val

420 425 430

Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu

435 440 445

Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Ile

450 455 460

Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala

465 470 475 480

Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly

485 490 495

Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro

500 505 510

Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro

515 520 525

Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln Ala Ser

530 535 540

Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu

545 550 555 560

Leu Gln Gln Glu Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser

565 570 575

Pro Asp Gln Ser His Gln Val Thr Gly Asn His Gln Gln Pro Pro Gln

580 585 590

Gln Asn Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr Tyr Asn Ser Arg

595 600 605

Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly

610 615 620

Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg

625 630 635 640

Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Thr Gln Ser Gln Phe

645 650 655

Ser Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln

660 665 670

Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg

675 680 685

Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln Met Asn

690 695 700

Thr Gln Gln Val Asn

705

<210> 3

<211> 3553

<212> DNA

<213> 人(Homo sapiens)

<220>

<221> CDS

<222> (190)..(2274)

<400> 3

cagagggctg ctggctggct aagtccctcc cgctcccggc tctcgcctca ctaggagcgg 60

ctctcggtgc agcgggacag ggcgaagcgg cctgcgccca cggagcgcgc gacactgccc 120

ggaagggacc gccacccttg ccccctcagc tgcccactcg tgatttccag cggcctccgc 180

gcgcgcacg atg ccc tcg gcc acc agc cac agc ggg agc ggc agc aag tcg 231

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser

1 5 10

tcc gga ccg cca ccg ccg tcg ggt tcc tcc ggg agt gag gcg gcc gcg 279

Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala

15 20 25 30

gga gcc ggg gcc gcc gcg ccg gct tct cag cac ccc gca acc ggc acc 327

Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr

35 40 45

ggc gct gtc cag acc gag gcc atg aag cag att ctc ggg gtg atc gac 375

Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp

50 55 60

aag aaa ctt cgg aac ctg gag aag aaa aag ggt aag ctt gat gat tac 423

Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr

65 70 75

cag gaa cga atg aac aaa ggg gaa agg ctt aat caa gat cag ctg gat 471

Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp

80 85 90

gcc gtt tct aag tac cag gaa gtc aca aat aat ttg gag ttt gca aaa 519

Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys

95 100 105 110

gaa tta cag agg agt ttc atg gca cta agt caa gat att cag aaa aca 567

Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr

115 120 125

ata aag aag aca gca cgt cgg gag cag ctt atg aga gaa gaa gct gaa 615

Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu

130 135 140

cag aaa cgt tta aaa act gta ctt gag cta cag tat gtt ttg gac aaa 663

Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys

145 150 155

ttg gga gat gat gaa gtg cgg act gac ctg aaa caa ggt ttg aat gga 711

Leu Gly Asp Asp Glu Val Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly

160 165 170

gtg cca ata ttg tcc gaa gag gag ttg tca ttg ttg gat gaa ttc tat 759

Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr

175 180 185 190

aag cta gta gac cct gaa cgg gac atg agc ttg agg ttg aat gaa cag 807

Lys Leu Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln

195 200 205

tat gaa cat gcc tcc att cac ctg tgg gac ctg ctg gaa ggg aag gaa 855

Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu

210 215 220

aaa cct gta tgt gga acc acc tat aaa gtt cta aag gaa att gtt gag 903

Lys Pro Val Cys Gly Thr Thr Tyr Lys Val Leu Lys Glu Ile Val Glu

225 230 235

cgt gtt ttt cag tca aac tac ttt gac agc acc cac aac cac cag aat 951

Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn

240 245 250

ggg ctg tgt gag gaa gaa gag gca gcc tca gca cct gca gtt gaa gac 999

Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Ala Val Glu Asp

255 260 265 270

cag gta cct gaa gct gaa cct gag cca gca gaa gag tac act gag caa 1047

Gln Val Pro Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln

275 280 285

agt gaa gtt gaa tca aca gag tat gta aat aga cag ttc atg gca gaa 1095

Ser Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu

290 295 300

aca cag ttc acc agt ggt gaa aag gag cag gta gat gag tgg aca gtt 1143

Thr Gln Phe Thr Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val

305 310 315

gaa acg gtt gag gtg gta aat tca ctc cag cag caa cct cag gct gca 1191

Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala

320 325 330

tcc cct tca gta cca gag ccc cac tct ttg act cca gtg gct cag gca 1239

Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ala

335 340 345 350

gat ccc ctt gtg aga aga cag cga gta caa gac ctt atg gca caa atg 1287

Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met

355 360 365

cag ggt ccc tat aat ttc ata cag gat tca atg ctg gat ttt gaa aat 1335

Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn

370 375 380

cag aca ctt gat cct gcc att gta tct gca cag cct atg aat cca aca 1383

Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr

385 390 395

caa aac atg gac atg ccc cag ctg gtt tgc cct cca gtt cat tct gaa 1431

Gln Asn Met Asp Met Pro Gln Leu Val Cys Pro Pro Val His Ser Glu

400 405 410

tct aga ctt gct cag cct aat caa gtt cct gta caa cca gaa gcg aca 1479

Ser Arg Leu Ala Gln Pro Asn Gln Val Pro Val Gln Pro Glu Ala Thr

415 420 425 430

cag gtt cct ttg gta tca tcc aca agt gag ggg tac aca gca tct caa 1527

Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln

435 440 445

ccc ttg tac cag cct tct cat gct aca gag caa cga cca cag aag gaa 1575

Pro Leu Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu

450 455 460

cca att gat cag att cag gca aca atc tct tta aat aca gac cag act 1623

Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr

465 470 475

aca gca tca tca tcc ctt cct gct gcg tct cag cct caa gta ttt cag 1671

Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln

480 485 490

gct ggg aca agc aaa cct tta cat agc agt gga atc aat gta aat gca 1719

Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala

495 500 505 510

gct cca ttc caa tcc atg caa acg gtg ttc aat atg aat gcc cca gtt 1767

Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val

515 520 525

cct cct gtt aat gaa cca gaa act tta aaa cag caa aat cag tac cag 1815

Pro Pro Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln

530 535 540

gcc agt tat aac cag agc ttt tct agt cag cct cac caa gta gaa caa 1863

Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln

545 550 555

aca gag ctt cag caa gaa cag ctt caa aca gtg gtt ggc act tac cat 1911

Thr Glu Leu Gln Gln Glu Gln Leu Gln Thr Val Val Gly Thr Tyr His

560 565 570

ggt tcc cca gac cag tcc cat caa gtg act ggt aac cac cag cag cct 1959

Gly Ser Pro Asp Gln Ser His Gln Val Thr Gly Asn His Gln Gln Pro

575 580 585 590

cct cag cag aac act gga ttt cca cgt agc aat cag ccc tat tac aat 2007

Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr Tyr Asn

595 600 605

agt cgt ggt gtg tct cgt gga ggc tcc cgt ggt gct aga ggc ttg atg 2055

Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met

610 615 620

aat gga tac cgg ggc cct gcc aat gga ttc aga gga gga tat gat ggt 2103

Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly

625 630 635

tac cgc cct tca ttc tct aac act cca aac agt ggt tat aca cag tct 2151

Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Thr Gln Ser

640 645 650

cag ttc agt gct ccc cgg gat tac tct ggc tat caa cgg gat gga tat 2199

Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr

655 660 665 670

cag cag aat ttc aag cga ggc tct ggg cag agt gga cca cgg gga gcc 2247

Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala

675 680 685

cca cga ggt aat att ttg tgg tgg tga tcctagctcc taagtggagc 2294

Pro Arg Gly Asn Ile Leu Trp Trp

690

ttctgttctg gccttggaag agctgttaat agtctgcatg ttaggaatac atttatcctt 2354

tccagacttg ttgctaggga ttaaatgaaa tgctctgttt ctaaaactta atcttggacc 2414

caaattttaa tttttgaatg atttaatttt ccctgttact atataaactg tcttgaaaac 2474

tagaacatat tctcttctca gaaaaagtgt ttttccaact gaaaattatt tttcaggtcc 2534

taaaacctgc taaatgtttt taggaagtac ttactgaaac atttttgtaa gacatttttg 2594

gaatgagatt gaacatttat ataaatttat tattcctctt tcattttttt gaaacatgcc 2654

tattatattt tagggccaga caccctttaa tggccggata agccatagtt aacatttaga 2714

gaaccattta gaagtgatag aactaatgga atttgcaatg ccttttggac ctctattagt 2774

gatataaata tcaagttatt tctgactttt aaacaaaact cccaaattcc taacttattg 2834

agctatactt aaaaaaaatt acaggtttag agagtttttt gtttttcttt tactgttgga 2894

aaactacttc ccattttggc aggaagttaa cctatttaac aattagagct agcatttcat 2954

gtagtctgaa attctaaatg gttctctgat ttgagggagg ttaaacatca aacaggtttc 3014

ctctattggc cataacatgt ataaaatgtg tgttaaggag gaattacaac gtactttgat 3074

ttgaatacta gtagaaactg gccaggaaaa aggtacattt ttctaaaaat taatggatca 3134

cttgggaatt actgacttga ctagaagtat caaaggatgt ttgcatgtga atgtgggtta 3194

tgttctttcc caccttgtag catattcgat gaaagttgag ttaactgata gctaaaaatc 3254

tgttttaaca gcatgtaaaa agttatttta tctgttaaaa gtcattatac agttttgaat 3314

gttatgtagt ttctttttaa cagtttaggt aataaggtct gttttcattc tggtgctttt 3374

attaattttg atagtatgat gttacttact actgaaatgt aagctagagt gtacactaga 3434

atgtaagctc catgagagca ggtaccttgt ctgtcttctc tgctgtatct attcccaacg 3494

cttgatgatg gtgcctggca catagtaggc actcaataaa tatttgttga atgaatgaa 3553

<210> 4

<211> 694

<212> PRT

<213> 人(Homo sapiens)

<400> 4

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala

35 40 45

Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys

50 55 60

Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu

65 70 75 80

Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val

85 90 95

Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu

100 105 110

Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys

115 120 125

Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys

130 135 140

Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly

145 150 155 160

Asp Asp Glu Val Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly Val Pro

165 170 175

Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu

180 185 190

Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu

195 200 205

His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro

210 215 220

Val Cys Gly Thr Thr Tyr Lys Val Leu Lys Glu Ile Val Glu Arg Val

225 230 235 240

Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu

245 250 255

Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Ala Val Glu Asp Gln Val

260 265 270

Pro Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu

275 280 285

Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln

290 295 300

Phe Thr Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr

305 310 315 320

Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro

325 330 335

Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ala Asp Pro

340 345 350

Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly

355 360 365

Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr

370 375 380

Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn

385 390 395 400

Met Asp Met Pro Gln Leu Val Cys Pro Pro Val His Ser Glu Ser Arg

405 410 415

Leu Ala Gln Pro Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val

420 425 430

Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu

435 440 445

Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Ile

450 455 460

Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala

465 470 475 480

Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly

485 490 495

Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro

500 505 510

Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro

515 520 525

Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln Ala Ser

530 535 540

Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu

545 550 555 560

Leu Gln Gln Glu Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser

565 570 575

Pro Asp Gln Ser His Gln Val Thr Gly Asn His Gln Gln Pro Pro Gln

580 585 590

Gln Asn Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr Tyr Asn Ser Arg

595 600 605

Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly

610 615 620

Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg

625 630 635 640

Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Thr Gln Ser Gln Phe

645 650 655

Ser Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln

660 665 670

Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg

675 680 685

Gly Asn Ile Leu Trp Trp

690

<210> 5

<211> 1605

<212> DNA

<213> 犬(Canis familiaris)

<220>

<221> CDS

<222> (46)..(1392)

<400> 5

gtcacaaata acttggagtt tgcaaaagaa ttacagagga gtttc atg gca tta agt 57

Met Ala Leu Ser

1

caa gat att cag aaa aca ata aag aag act gca cgt cgg gag cag ctt 105

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

5 10 15 20

atg aga gag gaa gcg gaa caa aaa cgt tta aaa act gta ctt gag ctc 153

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

25 30 35

cag tat gtt ttg gac aaa ttg gga gat gat gaa gtg aga act gac ctg 201

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

40 45 50

aag caa ggt ttg aat gga gtg cca ata ttg tct gaa gaa gaa ttg tcg 249

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

55 60 65

ttg ttg gat gaa ttc tac aaa tta gca gac cct gaa cgg gac atg agc 297

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

70 75 80

ttg agg ttg aat gag cag tat gaa cat gct tcc att cac ctg tgg gac 345

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

85 90 95 100

ttg ctg gaa gga aag gaa aag tct gta tgt gga aca acc tat aaa gca 393

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

105 110 115

cta aag gaa att gtt gag cgt gtt ttc cag tca aat tac ttt gac agc 441

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

120 125 130

act cac aac cac cag aat ggg cta tgt gag gaa gaa gag gca gcc tca 489

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

135 140 145

gca cct aca gtt gaa gac cag gta gct gaa gct gag cct gag cca gca 537

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

150 155 160

gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat gta aat 585

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

165 170 175 180

aga caa ttt atg gca gaa aca cag ttc agc agt ggt gaa aag gag cag 633

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

185 190 195

gta gat gag tgg acg gtc gaa aca gtg gag gtg gtg aat tca ctc cag 681

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

200 205 210

cag caa cct cag gct gcg tct cct tca gta cca gag ccc cac tct ttg 729

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

215 220 225

act ccg gtg gct cag gca gat ccc ctt gtg aga aga cag cga gtc cag 777

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

230 235 240

gac ctt atg gcg cag atg cag ggg ccc tat aat ttc ata cag gat tca 825

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

245 250 255 260

atg ctg gat ttt gaa aac cag aca ctc gat cct gcc att gta tct gca 873

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

265 270 275

cag cct atg aat ccg aca caa aac atg gac atg ccc cag ctg gtt tgc 921

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

280 285 290

cct cca gtt cat tct gaa tct aga ctt gct caa cct aat caa gtt cct 969

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

295 300 305

gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt gag 1017

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

310 315 320

ggg tat aca gca tct caa ccc ttg tac cag cct tct cat gct aca gag 1065

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

325 330 335 340

caa cga cca caa aag gaa cca att gac cag att cag gca aca atc tct 1113

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

345 350 355

tta aat aca gac cag act aca gcg tca tca tcc ctt ccg gct gct tct 1161

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

360 365 370

cag cct cag gta ttc cag gct ggg aca agc aaa cca tta cat agc agt 1209

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

375 380 385

gga atc aat gta aat gca gct cca ttc caa tcc atg caa acg gtg ttc 1257

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

390 395 400

aat atg aat gcc cca gtt cct cct gtt aat gaa cca gaa act ttg aaa 1305

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

405 410 415 420

caa caa aat cag tac cag gcc agt tat aac cag agc ttt tct agt cag 1353

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

425 430 435

cct cac caa gta gaa caa aca gag gga tgc cgc aaa tga acactcagca 1402

Pro His Gln Val Glu Gln Thr Glu Gly Cys Arg Lys

440 445

agtgaattaa tctgattcac aggattatgt ttaaacgcca aaaacacact ggccagtgta 1462

ccataatatg ttaccagaag agttattatc tatttgttct ccctttcagg aaacttattg 1522

taaagggact gttttcatcc cataaagaca ggactacaat tgtcagcttt atattacctg 1582

gaaaaaaaaa aaaaaaaaaa aaa 1605

<210> 6

<211> 448

<212> PRT

<213> 犬(Canis familiaris)

<400> 6

Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg

1 5 10 15

Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr

20 25 30

Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val

35 40 45

Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu

50 55 60

Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu

65 70 75 80

Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile

85 90 95

His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr

100 105 110

Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn

115 120 125

Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu

130 135 140

Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu

145 150 155 160

Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr

165 170 175

Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly

180 185 190

Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val

195 200 205

Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu

210 215 220

Pro His Ser Leu Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg

225 230 235 240

Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe

245 250 255

Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala

260 265 270

Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro

275 280 285

Gln Leu Val Cys Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro

290 295 300

Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser

305 310 315 320

Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser

325 330 335

His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln

340 345 350

Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu

355 360 365

Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro

370 375 380

Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met

385 390 395 400

Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro

405 410 415

Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser

420 425 430

Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Gly Cys Arg Lys

435 440 445

<210> 7

<211> 4154

<212> DNA

<213> 犬(Canis familiaris)

<220>

<221> CDS

<222> (1)..(2154)

<400> 7

atg ccg tcg gcc acc agc ctc agc gga agc ggc agc aag tcg tcg ggc 48

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccc ccg tcg ggt tcc tcc ggg agc gag gcg gcg gcg gcg gcg 96

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

ggg gcg gcg ggg gcg gcg ggg gcc ggg gcg gct gcg ccc gcc tcc cag 144

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

cac ccc gcg acc ggc acc ggc gct gtc cag acc gag gcc atg aag cag 192

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

atc ctc ggg gtg atc gac aag aaa ctc cgg aac ctg gag aag aaa aag 240

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

ggc aag ctt gat gat tac cag gaa cga atg aac aaa ggg gaa agg ctt 288

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

aat caa gat cag ctg gat gcc gta tct aag tac cag gaa gtc aca aat 336

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

aac ttg gag ttt gca aaa gaa tta cag agg agt ttc atg gca tta agt 384

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

caa gat att cag aaa aca ata aag aag act gca cgt cgg gag cag ctt 432

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

atg aga gag gaa gcg gaa caa aaa cgt tta aaa act gta ctt gag ctc 480

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

cag tat gtt ttg gac aaa ttg gga gat gat gaa gtg aga act gac ctg 528

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

aag caa ggt ttg aat gga gtg cca ata ttg tct gaa gaa gaa ttg tcg 576

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

ttg ttg gat gaa ttc tac aaa tta gca gac cct gaa cgg gac atg agc 624

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

ttg agg ttg aat gag cag tat gaa cat gct tcc att cac ctg tgg gac 672

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

ttg ctg gaa gga aag gaa aag tct gta tgt gga aca acc tat aaa gca 720

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

cta aag gaa att gtt gag cgt gtt ttc cag tca aat tac ttt gac agc 768

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

act cac aac cac cag aat ggg cta tgt gag gaa gaa gag gca gcc tca 816

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

gca cct aca gtt gaa gac cag gta gct gaa gct gag cct gag cca gca 864

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat gta aat 912

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

aga caa ttt atg gca gaa aca cag ttc agc agt ggt gaa aag gag cag 960

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

gta gat gag tgg acg gtc gaa aca gtg gag gtg gtg aat tca ctc cag 1008

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

cag caa cct cag gct gcg tct cct tca gta cca gag ccc cac tct ttg 1056

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

act ccg gtg gct cag gca gat ccc ctt gtg aga aga cag cga gtc cag 1104

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

gac ctt atg gcg cag atg cag ggg ccc tat aat ttc ata cag gat tca 1152

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

atg ctg gat ttt gaa aac cag aca ctc gat cct gcc att gta tct gca 1200

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

cag cct atg aat ccg aca caa aac atg gac atg ccc cag ctg gtt tgc 1248

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

cct cca gtt cat tct gaa tct aga ctt gct caa cct aat caa gtt cct 1296

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt gag 1344

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

ggg tat aca gca tct caa ccc ttg tac cag cct tct cat gct aca gag 1392

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

caa cga cca caa aag gaa cca att gac cag att cag gca aca atc tct 1440

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

tta aat aca gac cag act aca gcg tca tca tcc ctt ccg gct gct tct 1488

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

cag cct cag gta ttc cag gct ggg aca agc aaa cca tta cat agc agt 1536

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

gga atc aat gta aat gca gct cca ttc caa tcc atg caa acg gtg ttc 1584

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

aat atg aat gcc cca gtt cct cct gtt aat gaa cca gaa act ttg aaa 1632

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

caa caa aat cag tac cag gcc agt tat aac cag agc ttt tct agt cag 1680

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

cct cac caa gta gaa caa aca gac ctt cag caa gaa cag ctt caa aca 1728

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

gtg gtt ggc act tac cat ggt tcc cag gac cag ccc cac caa gtg act 1776

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

ggt aac cat cag cag cct ccc cag cag aac act gga ttt cca cgt agc 1824

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

agt cag ccc tat tac aat agt cgt ggt gtg tct cgt ggt ggt tcc cgt 1872

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

ggt gct aga ggc tta atg aat gga tac agg ggc cct gcc aat gga ttc 1920

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

aga gga gga tat gat ggt tac cgc cct tca ttc tct aac act cca aac 1968

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

agt ggt tat aca cag tct cag ttc agt gct ccc cgg gac tac tct ggc 2016

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

tat cag cgg gat gga tat cag cag aat ttc aag cga ggc tct ggg cag 2064

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

agt gga cca cgg gga gcc cca cga ggt cgt gga ggg ccc cca aga ccc 2112

Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro

690 695 700

aac aga ggg atg ccg caa atg aac act cag caa gtg aat taa 2154

Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

705 710 715

tctgattcac aggattatgt ttaaacgcca aaaacacact ggccagtgta ccataatatg 2214

ttaccagaag agttattatc tatttgttct ccctttcagg aaacttattg taaagggact 2274

gttttcatcc cataaagaca ggactacaat tgtcagcttt atattacctg gatatggaag 2334

gaaactattt ttattctgca tgttcttcct aagcgtcatc ttgagccttg cacatgatac 2394

tcagattcct cacccttgct taggagtaaa acataataca ctttacaggg tgatatctcc 2454

atagttattt gaagtggctt ggaaaaagca agattaactt ctgacattgg ataaaaatca 2514

acaaatcagc cctagagtta ttcaaatggt aattgacaaa aactaaaata tttcccttcg 2574

agaaggagtg gaatgtggtt tggcagaaca actgcatttc acagcttttc cggttaaatt 2634

ggagcactaa acgtttagat gcataccaaa ttatgcatgg gcccttaata taaaaggctg 2694

gctaccagct ttgacacagc actattcatc ctctggccaa acaactgtgg ttaaacaaca 2754

catgtaaatt gctttttaac agctgatact ataataagac aaagccaaaa tgcaaaaatt 2814

gggctttgat tggcactttt tgaaaaatat gcaacaaata tgggatgtaa tctggatggc 2874

cgcttctgta cttaatgtga agtatttaga tacctttttg aacacttaac agtttcttct 2934

gacaatgact tttgtaagga ttggtactat ctatcattcc ttataatgta cattgtctgt 2994

cactaatcct cagatcttgc tgtattgtca cctaaattgg tacaggtact gatgaaaata 3054

tctaatggat aatcataaca ctcttggtca catgtttttc ctgcagcctg aaggttttta 3114

aaagaaaaag atatcaaatg cctgctgcta ccaccctttt aaattgctat cttttgaaaa 3174

gcaccagtat gtgttttaga ttgatttccc tattttaggg aaatgacaga cagtagtttc 3234

agttctgatg gtataagcaa aacaaataaa acatgtttat aaaagttgta tcttgaaaca 3294

ctggtgttca acagctagca gcttatgtgg ttcaccccat gcattgttag tgtttcagat 3354

tttatggtta tctccagcag ctgtttctgt agtacttgca tttatctttt gtctaaccct 3414

aatattctca cggaggcatt tatattcaaa gtggtgatcc cttcacttag acgcataggg 3474

agagtcacaa gtttgatgaa gaggacagtg tagtaattta tatgctgttg gaatttgtgc 3534

tagcagtttg agcactagtt ctgtgtgcct atgaacttaa tgctgcttgt catattccac 3594

tttgacttca tggagaatta atcccatcta ctcagcaaag gctatactaa tactaagtta 3654

atggtatttt ctgtgcagaa attgaatttt gttttattag catttagcta aggaattttt 3714

ccagtaggtg ctcagctact aaagaaaaac aaaaacaaga cacaaaacta ttctcaaaca 3774

ttcattgtta gacaactgga gtttttgctg gttttgtaac ctactaaaat ggataggctg 3834

ttgaacattc cacattcaaa agttttttgt agggtggtgg ggaagggggg gtgtcttcaa 3894

tgtttatttt aaaataaaat aagttcttga cttttctcat gtgtggttgt ggtacatcat 3954

attggaaggg ttatctgttt acttttgcaa atgagtattt ctcttgctag cacctcccgt 4014

tgtgcgcttt aaatgacatc tgcctgggat gtaccacaac catatgttag ctgtatttta 4074

tggggaatag ataaaatatt cgtggtttat tgggtaatcc ctagatgtgt atgcttacaa 4134

tcctatatat aaaactaaat 4154

<210> 8

<211> 717

<212> PRT

<213> 犬(Canis familiaris)

<400> 8

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro

690 695 700

Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

705 710 715

<210> 9

<211> 4939

<212> DNA

<213> 犬(Canis familiaris)

<220>

<221> CDS

<222> (1)..(2109)

<400> 9

atg ccg tcg gcc acc agc ctc agc gga agc ggc agc aag tcg tcg ggc 48

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccc ccg tcg ggt tcc tcc ggg agc gag gcg gcg gcg gcg gcg 96

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

ggg gcg gcg ggg gcg gcg ggg gcc ggg gcg gct gcg ccc gcc tcc cag 144

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

cac ccc gcg acc ggc acc ggc gct gtc cag acc gag gcc atg aag cag 192

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

atc ctc ggg gtg atc gac aag aaa ctc cgg aac ctg gag aag aaa aag 240

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

ggc aag ctt gat gat tac cag gaa cga atg aac aaa ggg gaa agg ctt 288

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

aat caa gat cag ctg gat gcc gta tct aag tac cag gaa gtc aca aat 336

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

aac ttg gag ttt gca aaa gaa tta cag agg agt ttc atg gca tta agt 384

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

caa gat att cag aaa aca ata aag aag act gca cgt cgg gag cag ctt 432

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

atg aga gag gaa gcg gaa caa aaa cgt tta aaa act gta ctt gag ctc 480

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

cag tat gtt ttg gac aaa ttg gga gat gat gaa gtg aga act gac ctg 528

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

aag caa ggt ttg aat gga gtg cca ata ttg tct gaa gaa gaa ttg tcg 576

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

ttg ttg gat gaa ttc tac aaa tta gca gac cct gaa cgg gac atg agc 624

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

ttg agg ttg aat gag cag tat gaa cat gct tcc att cac ctg tgg gac 672

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

ttg ctg gaa gga aag gaa aag tct gta tgt gga aca acc tat aaa gca 720

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

cta aag gaa att gtt gag cgt gtt ttc cag tca aat tac ttt gac agc 768

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

act cac aac cac cag aat ggg cta tgt gag gaa gaa gag gca gcc tca 816

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

gca cct aca gtt gaa gac cag gta gct gaa gct gag cct gag cca gca 864

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat gta aat 912

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

aga caa ttt atg gca gaa aca cag ttc agc agt ggt gaa aag gag cag 960

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

gta gat gag tgg acg gtc gaa aca gtg gag gtg gtg aat tca ctc cag 1008

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

cag caa cct cag gct gcg tct cct tca gta cca gag ccc cac tct ttg 1056

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

act ccg gtg gct cag gca gat ccc ctt gtg aga aga cag cga gtc cag 1104

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

gac ctt atg gcg cag atg cag ggg ccc tat aat ttc ata cag gat tca 1152

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

atg ctg gat ttt gaa aac cag aca ctc gat cct gcc att gta tct gca 1200

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

cag cct atg aat ccg aca caa aac atg gac atg ccc cag ctg gtt tgc 1248

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

cct cca gtt cat tct gaa tct aga ctt gct caa cct aat caa gtt cct 1296

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt gag 1344

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

ggg tat aca gca tct caa ccc ttg tac cag cct tct cat gct aca gag 1392

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

caa cga cca caa aag gaa cca att gac cag att cag gca aca atc tct 1440

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

tta aat aca gac cag act aca gcg tca tca tcc ctt ccg gct gct tct 1488

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

cag cct cag gta ttc cag gct ggg aca agc aaa cca tta cat agc agt 1536

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

gga atc aat gta aat gca gct cca ttc caa tcc atg caa acg gtg ttc 1584

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

aat atg aat gcc cca gtt cct cct gtt aat gaa cca gaa act ttg aaa 1632

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

caa caa aat cag tac cag gcc agt tat aac cag agc ttt tct agt cag 1680

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

cct cac caa gta gaa caa aca gac ctt cag caa gaa cag ctt caa aca 1728

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

gtg gtt ggc act tac cat ggt tcc cag gac cag ccc cac caa gtg act 1776

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

ggt aac cat cag cag cct ccc cag cag aac act gga ttt cca cgt agc 1824

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

agt cag ccc tat tac aat agt cgt ggt gtg tct cgt ggt ggt tcc cgt 1872

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

ggt gct aga ggc tta atg aat gga tac agg ggc cct gcc aat gga ttc 1920

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

aga gga gga tat gat ggt tac cgc cct tca ttc tct aac act cca aac 1968

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

agt ggt tat aca cag tct cag ttc agt gct ccc cgg gac tac tct ggc 2016

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

tat cag cgg gat gga tat cag cag aat ttc aag cga ggc tct ggg cag 2064

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

agt gga cca cgg gga gcc cca cga ggt aat att ttg tgg tgg tga 2109

Ser Gly Pro Arg Gly Ala Pro Arg Gly Asn Ile Leu Trp Trp

690 695 700

tcctagctcc taagtggagc ttctgttctg gccttggaag agctgttcca tagtctgcat 2169

gtaggttaca tgttaggaat acatttatca ttaccagact tgttgctagg gattaaatga 2229

aatgctctgt ttctaaaact tctcttgaac ccaaatttaa ttttttgaat gactttccct 2289

gttactatat aaattgtctt gaaaactaga acatttctcc tcctcagaaa aagtgttttt 2349

ccaactgcaa attatttttc aggtcctaaa acctgctaaa tgtttttagg aagtacttac 2409

tgaaacattt ttgtaagaca tttttggaat gagattgaac atttatataa atttattatt 2469

attcctcttt catttttgaa catgcatatt atattttagg gtcagaaatc ctttaatggc 2529

caaataagcc atagttacat ttagagaacc atttagaagt gatagaacta actgaaattt 2589

caatgccttt ggatcattaa tagcgatata aatttcaaat tgtttctgac ttttaaataa 2649

aacatccaaa atcctaacta acttcctgaa ctatatttaa aaattacagg tttaaggagt 2709

ttctggtttt ttttctctta ccataggaaa actgtttcct gtttggccag gaagtcaacc 2769

tgtgtaataa ttagaagtag catttcatat gatctgaagt tctaaatggt tctctgattt 2829

aagggaagtt aaattgaata ggtttcctct agttattggc cataacatgt ataaaatgta 2889

tattaaggag gaatacaaag tactttgatt tcaatgctag tagaaactgg ccagcaaaaa 2949

ggtgcatttt atttttaaat taatggatca cttgggaatt actgacttga agtatcaaag 3009

gatatttgca tgtgaatgtg ggttatgttc tttctcacct tgtagcatat tctatgaaag 3069

ttgagttgac tggtagctaa aaatctgttt taacagcatg taaaaagtta ttttatctgt 3129

tacaagtcat tatacaattt tgaatgttat gtagtttctt tttaacagtt taggtaacaa 3189

ggtctgtttt tcattctggt gcttttatta attttgatag tatgatgtta cttactactg 3249

aaatgtaagc tagagtgtac actagaatgt aagctccatg agagcaggta ccttgtctgt 3309

cttcactgct gtatctattt ccaacgcctg atgacagtgc ctgacacata gtaggcactc 3369

aataaatact tgttgaatga atgaatgaat gagtactggt ggaatactcc attagctcta 3429

ctcttctttt agctagagaa catgagcaaa tttgcgcatg acaacttcca ggacaggtga 3489

acactgaaga attgacctct taaacctaat aatgtggtga caagctgccc acatgcttct 3549

tgacttcaga tgaaaatctg cttgaaggca aagcaaataa tatttgaaag aaaaaccaaa 3609

tgccattttt gtcttctagg tcgtggaggg cccccaagac ccaacagagg gatgccgcaa 3669

atgaacactc agcaagtgaa ttaatctgat tcacaggatt atgtttaaac gccaaaaaca 3729

cactggccag tgtaccataa tatgttacca gaagagttat tatctatttg ttctcccttt 3789

caggaaactt attgtaaagg gactgttttc atcccataaa gacaggacta caattgtcag 3849

ctttatatta cctggatatg gaaggaaact atttttattc tgcatgttct tcctaagcgt 3909

catcttgagc cttgcacatg atactcagat tcctcaccct tgcttaggag taaaacataa 3969

tacactttac agggtgatat ctccatagtt atttgaagtg gcttggaaaa agcaagatta 4029

acttctgaca ttggataaaa atcaacaaat cagccctaga gttattcaaa tggtaattga 4089

caaaaactaa aatatttccc ttcgagaagg agtggaatgt ggtttggcag aacaactgca 4149

tttcacagct tttccggtta aattggagca ctaaacgttt agatgcatac caaattatgc 4209

atgggccctt aatataaaag gctggctacc agctttgaca cagcactatt catcctctgg 4269

ccaaacaact gtggttaaac aacacatgta aattgctttt taacagctga tactataata 4329

agacaaagcc aaaatgcaaa aattgggctt tgattggcac tttttgaaaa atatgcaaca 4389

aatatgggat gtaatctgga tggccgcttc tgtacttaat gtgaagtatt tagatacctt 4449

tttgaacact taacagtttc ttctgacaat gacttttgta aggattggta ctatctatca 4509

ttccttataa tgtacattgt ctgtcactaa tcctcagatc ttgctgtatt gtcacctaaa 4569

ttggtacagg tactgatgaa aatatctaat ggataatcat aacactcttg gtcacatgtt 4629

tttcctgcag cctgaaggtt tttaaaagaa aaagatatca aatgcctgct gctaccaccc 4689

ttttaaattg ctatcttttg aaaagcacca gtatgtgttt tagattgatt tccctatttt 4749

agggaaatga cagacagtag tttcagttct gatggtataa gcaaaacaaa taaaacatgt 4809

ttataaaagt tgtatcttga aacactggtg ttcaacagct agcagcttat gtggttcacc 4869

ccatgcattg ttagtgtttc agattttatg gttatctcca gcagctgttt ctgtagtact 4929

tgcatttatc 4939

<210> 10

<211> 702

<212> PRT

<213> 犬(Canis familiaris)

<400> 10

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

Ser Gly Pro Arg Gly Ala Pro Arg Gly Asn Ile Leu Trp Trp

690 695 700

<210> 11

<211> 3306

<212> DNA

<213> 犬(Canis familiaris)

<220>

<221> CDS

<222> (1)..(2040)

<400> 11

atg ccg tcg gcc acc agc ctc agc gga agc ggc agc aag tcg tcg ggc 48

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccc ccg tcg ggt tcc tcc ggg agc gag gcg gcg gcg gcg gcg 96

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

ggg gcg gcg ggg gcg gcg ggg gcc ggg gcg gct gcg ccc gcc tcc cag 144

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

cac ccc gcg acc ggc acc ggc gct gtc cag acc gag gcc atg aag cag 192

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

atc ctc ggg gtg atc gac aag aaa ctc cgg aac ctg gag aag aaa aag 240

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

ggc aag ctt gat gat tac cag gaa cga atg aac aaa ggg gaa agg ctt 288

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

aat caa gat cag ctg gat gcc gta tct aag tac cag gaa gtc aca aat 336

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

aac ttg gag ttt gca aaa gaa tta cag agg agt ttc atg gca tta agt 384

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

caa gat att cag aaa aca ata aag aag act gca cgt cgg gag cag ctt 432

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

atg aga gag gaa gcg gaa caa aaa cgt tta aaa act gta ctt gag ctc 480

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

cag tat gtt ttg gac aaa ttg gga gat gat gaa gtg aga act gac ctg 528

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

aag caa ggt ttg aat gga gtg cca ata ttg tct gaa gaa gaa ttg tcg 576

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

ttg ttg gat gaa ttc tac aaa tta gca gac cct gaa cgg gac atg agc 624

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

ttg agg ttg aat gag cag tat gaa cat gct tcc att cac ctg tgg gac 672

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

ttg ctg gaa gga aag gaa aag tct gta tgt gga aca acc tat aaa gca 720

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

cta aag gaa att gtt gag cgt gtt ttc cag tca aat tac ttt gac agc 768

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

act cac aac cac cag aat ggg cta tgt gag gaa gaa gag gca gcc tca 816

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

gca cct aca gtt gaa gac cag gta gct gaa gct gag cct gag cca gca 864

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat gta aat 912

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

aga caa ttt atg gca gaa aca cag ttc agc agt ggt gaa aag gag cag 960

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

gta gat gag tgg acg gtc gaa aca gtg gag gtg gtg aat tca ctc cag 1008

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

cag caa cct cag gct gcg tct cct tca gta cca gag ccc cac tct ttg 1056

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

act ccg gtg gct cag gca gat ccc ctt gtg aga aga cag cga gtc cag 1104

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

gac ctt atg gcg cag atg cag ggg ccc tat aat ttc ata cag gat tca 1152

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

atg ctg gat ttt gaa aac cag aca ctc gat cct gcc att gta tct gca 1200

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

cag cct atg aat ccg aca caa aac atg gac atg ccc cag ctg gtt tgc 1248

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

cct cca gtt cat tct gaa tct aga ctt gct caa cct aat caa gtt cct 1296

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt gag 1344

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

ggg tat aca gca tct caa ccc ttg tac cag cct tct cat gct aca gag 1392

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

caa cga cca caa aag gaa cca att gac cag att cag gca aca atc tct 1440

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

tta aat aca gac cag act aca gcg tca tca tcc ctt ccg gct gct tct 1488

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

cag cct cag gta ttc cag gct ggg aca agc aaa cca tta cat agc agt 1536

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

gga atc aat gta aat gca gct cca ttc caa tcc atg caa acg gtg ttc 1584

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

aat atg aat gcc cca gtt cct cct gtt aat gaa cca gaa act ttg aaa 1632

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

caa caa aat cag tac cag gcc agt tat aac cag agc ttt tct agt cag 1680

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

cct cac caa gta gaa caa aca gac ctt cag caa gaa cag ctt caa aca 1728

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

gtg gtt ggc act tac cat ggt tcc cag gac cag ccc cac caa gtg act 1776

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

ggt aac cat cag cag cct ccc cag cag aac act gga ttt cca cgt agc 1824

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

agt cag ccc tat tac aat agt cgt ggt gtg tct cgt ggt ggt tcc cgt 1872

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

ggt gct aga ggc tta atg aat gga tac agg ggc cct gcc aat gga ttc 1920

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

aga gga gga tat gat ggt tac cgc cct tca ttc tct aac act cca aac 1968

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

agt ggt tat aca cag tct cag ttc agt gct ccc cgg gac tac tct ggc 2016

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

tat cag cgg gga tgc cgc aaa tga acactcagca agtgaattaa tctgattcac 2070

Tyr Gln Arg Gly Cys Arg Lys

675

aggattatgt ttaaacgcca aaaacacact ggccagtgta ccataatatg ttaccagaag 2130

agttattatc tatttgttct ccctttcagg aaacttattg taaagggact gttttcatcc 2190

cataaagaca ggactacaat tgtcagcttt atattacctg gatatggaag gaaactattt 2250

ttattctgca tgttcttcct aagcgtcatc ttgagccttg cacatgatac tcagattcct 2310

cacccttgct taggagtaaa acataataca ctttacaggg tgatatctcc atagttattt 2370

gaagtggctt ggaaaaagca agattaactt ctgacattgg ataaaaatca acaaatcagc 2430

cctagagtta ttcaaatggt aattgacaaa aactaaaata tttcccttcg agaaggagtg 2490

gaatgtggtt tggcagaaca actgcatttc acagcttttc cggttaaatt ggagcactaa 2550

acgtttagat gcataccaaa ttatgcatgg gcccttaata taaaaggctg gctaccagct 2610

ttgacacagc actattcatc ctctggccaa acaactgtgg ttaaacaaca catgtaaatt 2670

gctttttaac agctgatact ataataagac aaagccaaaa tgcaaaaatt gggctttgat 2730

tggcactttt tgaaaaatat gcaacaaata tgggatgtaa tctggatggc cgcttctgta 2790

cttaatgtga agtatttaga tacctttttg aacacttaac agtttcttct gacaatgact 2850

tttgtaagga ttggtactat ctatcattcc ttataatgta cattgtctgt cactaatcct 2910

cagatcttgc tgtattgtca cctaaattgg tacaggtact gatgaaaata tctaatggat 2970

aatcataaca ctcttggtca catgtttttc ctgcagcctg aaggttttta aaagaaaaag 3030

atatcaaatg cctgctgcta ccaccctttt aaattgctat cttttgaaaa gcaccagtat 3090

gtgttttaga ttgatttccc tattttaggg aaatgacaga cagtagtttc agttctgatg 3150

gtataagcaa aacaaataaa acatgtttat aaaagttgta tcttgaaaca ctggtgttca 3210

acagctagca gcttatgtgg ttcaccccat gcattgttag tgtttcagat tttatggtta 3270

tctccagcag ctgtttctgt agtacttgca tttatc 3306

<210> 12

<211> 679

<212> PRT

<213> 犬(Canis familiaris)

<400> 12

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

Tyr Gln Arg Gly Cys Arg Lys

675

<210> 13

<211> 2281

<212> DNA

<213> 犬(Canis familiaris)

<220>

<221> CDS

<222> (1)..(2154)

<400> 13

atg ccg tcg gcc acc agc ctc agc gga agc ggc agc aag tcg tcg ggc 48

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccc ccg tcg ggt tcc tcc ggg agc gag gcg gcg gcg gcg gcg 96

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

ggg gcg gcg ggg gcg gcg ggg gcc ggg gcg gct gcg ccc gcc tcc cag 144

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

cac ccc gcg acc ggc acc ggc gct gtc cag acc gag gcc atg aag cag 192

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

atc ctc ggg gtg atc gac aag aaa ctc cgg aac ctg gag aag aaa aag 240

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

ggc aag ctt gat gat tac cag gaa cga atg aac aaa ggg gaa agg ctt 288

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

aat caa gat cag ctg gat gcc gta tct aag tac cag gaa gtc aca aat 336

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

aac ttg gag ttt gca aaa gaa tta cag agg agt ttc atg gca tta agt 384

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

caa gat att cag aaa aca ata aag aag act gca cgt cgg gag cag ctt 432

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

atg aga gag gaa gcg gaa caa aaa cgt tta aaa act gta ctt gag ctc 480

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

cag tat gtt ttg gac aaa ttg gga gat gat gaa gtg aga act gac ctg 528

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

aag caa ggt ttg aat gga gtg cca ata ttg tct gaa gaa gaa ttg tcg 576

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

ttg ttg gat gaa ttc tac aaa tta gca gac cct gaa cgg gac atg agc 624

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

ttg agg ttg aat gag cag tat gaa cat gct tcc att cac ctg tgg gac 672

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

ttg ctg gaa gga aag gaa aag tct gta tgt gga aca acc tat aaa gca 720

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

cta aag gaa att gtt gag cgt gtt ttc cag tca aat tac ttt gac agc 768

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

act cac aac cac cag aat ggg cta tgt gag gaa gaa gag gca gcc tca 816

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

gca cct aca gtt gaa gac cag gta gct gaa gct gag cct gag cca gca 864

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat gta aat 912

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

aga caa ttt atg gca gaa aca cag ttc agc agt ggt gaa aag gag cag 960

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

gta gat gag tgg acg gtc gaa aca gtg gag gtg gtg aat tca ctc cag 1008

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

cag caa cct cag gct gcg tct cct tca gta cca gag ccc cac tct ttg 1056

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

act ccg gtg gct cag gca gat ccc ctt gtg aga aga cag cga gtc cag 1104

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

gac ctt atg gcg cag atg cag ggg ccc tat aat ttc ata cag gat tca 1152

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

atg ctg gat ttt gaa aac cag aca ctc gat cct gcc att gta tct gca 1200

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

cag cct atg aat ccg aca caa aac atg gac atg ccc cag ctg gtt tgc 1248

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

cct cca gtt cat tct gaa tct aga ctt gct caa cct aat caa gtt cct 1296

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt gag 1344

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

ggg tat aca gca tct caa ccc ttg tac cag cct tct cat gct aca gag 1392

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

caa cga cca caa aag gaa cca att gac cag att cag gca aca atc tct 1440

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

tta aat aca gac cag act aca gcg tca tca tcc ctt ccg gct gct tct 1488

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

cag cct cag gta ttc cag gct ggg aca agc aaa cca tta cat agc agt 1536

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

gga atc aat gta aat gca gct cca ttc caa tcc atg caa acg gtg ttc 1584

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

aat atg aat gcc cca gtt cct cct gtt aat gaa cca gaa act ttg aaa 1632

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

caa caa aat cag tac cag gcc agt tat aac cag agc ttt tct agt cag 1680

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

cct cac caa gta gaa caa aca gac ctt cag caa gaa cag ctt caa aca 1728

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

gtg gtt ggc act tac cat ggt tcc cag gac cag ccc cac caa gtg act 1776

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

ggt aac cat cag cag cct ccc cag cag aac act gga ttt cca cgt agc 1824

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

agt cag ccc tat tac aat agt cgt ggt gtg tct cgt ggt ggt tcc cgt 1872

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

ggt gct aga ggc tta atg aat gga tac agg ggc cct gcc aat gga ttc 1920

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

aga gga gga tat gat ggt tac cgc cct tca ttc tct aac act cca aac 1968

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

agt ggt tat aca cag tct cag ttc agt gct ccc cgg gac tac tct ggc 2016

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

tat cag cgg gat gga tat cag cag aat ttc aag cga ggc tct ggg cag 2064

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

agt gga cca cgg gga gcc cca cga ggt cgt gga ggg ccc cca aga ccc 2112

Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro

690 695 700

aac aga ggg atg ccg caa atg aac act cag caa gtg aat taa 2154

Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

705 710 715

tctgattcac aggattatgt ttaaacgcca aaaacacact ggccagtgta ccataatatg 2214

ttaccagaag agttattatc tatttggact gttttcatcc cataaagaca ggactacaat 2274

tgtcagc 2281

<210> 14

<211> 717

<212> PRT

<213> 犬(Canis familiaris)

<400> 14

Met Pro Ser Ala Thr Ser Leu Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Ala Ala

20 25 30

Gly Ala Ala Gly Ala Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln

35 40 45

His Pro Ala Thr Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln

50 55 60

Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys

65 70 75 80

Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu

85 90 95

Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn

100 105 110

Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser

115 120 125

Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu

130 135 140

Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu

145 150 155 160

Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu

165 170 175

Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser

180 185 190

Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser

195 200 205

Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp

210 215 220

Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr Lys Ala

225 230 235 240

Leu Lys Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser

245 250 255

Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser

260 265 270

Ala Pro Thr Val Glu Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala

275 280 285

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

290 295 300

Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln

305 310 315 320

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

325 330 335

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu

340 345 350

Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln

355 360 365

Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser

370 375 380

Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala

385 390 395 400

Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys

405 410 415

Pro Pro Val His Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Pro

420 425 430

Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu

435 440 445

Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu

450 455 460

Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser

465 470 475 480

Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser

485 490 495

Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser

500 505 510

Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe

515 520 525

Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys

530 535 540

Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln

545 550 555 560

Pro His Gln Val Glu Gln Thr Asp Leu Gln Gln Glu Gln Leu Gln Thr

565 570 575

Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr

580 585 590

Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser

595 600 605

Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg

610 615 620

Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe

625 630 635 640

Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn

645 650 655

Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly

660 665 670

Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln

675 680 685

Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro

690 695 700

Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

705 710 715

<210> 15

<211> 3386

<212> DNA

<213> 牛(Bos taurus)

<220>

<221> CDS

<222> (82)..(2208)

<400> 15

cgcgtctcgc cccgtccacc gattgactcg ccgctcttgt ccttcctccc gctctttctt 60

ctctcccctt acggtttcaa g atg cct tcg gcc acc agc cac agc gga agc 111

Met Pro Ser Ala Thr Ser His Ser Gly Ser

1 5 10

ggc agc aag tcg tcc gga ccg cca ccg ccg tcg ggt tcc tcc ggg aat 159

Gly Ser Lys Ser Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Asn

15 20 25

gag gcg ggg gcc ggg gcc gcc gcg ccg gct tcc caa cac ccc atg acc 207

Glu Ala Gly Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Met Thr

30 35 40

ggc acc ggg gct gtc cag acc gag gcc atg aag cag att ctc ggg gtg 255

Gly Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val

45 50 55

atc gac aag aaa ctt cgg aac ctg gag aag aaa aag ggc aag ctt gat 303

Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp

60 65 70

gat tat cag gaa cga atg aac aaa ggg gaa agg ctt aat caa gat cag 351

Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln

75 80 85 90

ctg gat gcc gtg tct aag tac cag gaa gtc aca aat aac ttg gag ttt 399

Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe

95 100 105

gca aaa gaa tta cag agg agt ttc atg gca tta agc caa gat att cag 447

Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln

110 115 120

aaa aca ata aag aag aca gca cgt cgg gag cag ctt atg aga gag gaa 495

Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu

125 130 135

gct gaa cag aaa cgt tta aaa aca gta ctt gag ctg cag tat gtt ttg 543

Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu

140 145 150

gac aaa cta gga gat gat gaa gtg aga act gac ctg aag caa ggt ttg 591

Asp Lys Leu Gly Asp Asp Glu Val Arg Thr Asp Leu Lys Gln Gly Leu

155 160 165 170

aat gga gtg cca ata ttg tct gaa gag gag ttg tcg ttg tta gat gag 639

Asn Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu

175 180 185

ttc tac aaa tta gca gac cct gaa cga gac atg agc ttg agg ttg aat 687

Phe Tyr Lys Leu Ala Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn

190 195 200

gag cag tat gaa cat gcc tcc att cac ctg tgg gac ttg ctg gaa gga 735

Glu Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly

205 210 215

aag gaa aaa cct gta tgt gga aca act tat aaa gct cta aag gaa att 783

Lys Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile

220 225 230

gtt gag cgt gtt ttc cag tca aac tac ttt gac agc acc cac aac cac 831

Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His

235 240 245 250

cag aat ggt ctg tgt gag gaa gag gag gca gcc tca gca cct aca gtt 879

Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Thr Val

255 260 265

gaa gac cag gca gct gaa gct gaa cct gag cca gtg gaa gaa tat act 927

Glu Asp Gln Ala Ala Glu Ala Glu Pro Glu Pro Val Glu Glu Tyr Thr

270 275 280

gaa caa aat gag gtt gaa tca aca gag tat gta aat aga caa ttt atg 975

Glu Gln Asn Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met

285 290 295

gca gaa aca cag ttc agc agt ggt gaa aag gag cag gta gat gat tgg 1023

Ala Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln Val Asp Asp Trp

300 305 310

aca gtt gaa aca gtt gag gtg gta aat tca ctc cag cag caa cct cag 1071

Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln

315 320 325 330

gct gca tct cct tca gta cca gaa ccc cac tct ttg acc cca gtg gct 1119

Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala

335 340 345

caa gcc gat ccc ctc gtg aga aga cag cga gta cag gac ctt atg gca 1167

Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala

350 355 360

caa atg cag ggg ccc tat aat ttc ata cag gat tca atg ttg gat ttt 1215

Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe

365 370 375

gaa aac cag aca ctt gat cct gcc att gta tct gca cag ccg atg aat 1263

Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn

380 385 390

cca gca cag aac atg gac ata ccc cag ctg gtt tgc cct cca gtt cat 1311

Pro Ala Gln Asn Met Asp Ile Pro Gln Leu Val Cys Pro Pro Val His

395 400 405 410

tct gaa tct aga ctt gct caa cct aat caa gtt tct gta cag cca gaa 1359

Ser Glu Ser Arg Leu Ala Gln Pro Asn Gln Val Ser Val Gln Pro Glu

415 420 425

gct aca cag gtt cct ttg gtt tca tcc aca agt gag gga tat aca gca 1407

Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala

430 435 440

tct caa ccc ttg tac caa cct tct cat gct act gac caa cga cca caa 1455

Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Asp Gln Arg Pro Gln

445 450 455

aag gaa ccg att gat cag att cag gcg acg atc tct tta aat aca gac 1503

Lys Glu Pro Ile Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp

460 465 470

cag act aca gca tca tca tcc ctt cct gct gct tct cag cct caa gtg 1551

Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val

475 480 485 490

ttc cag gct ggg aca agc aaa cct tta cat agc agt gga atc aat gta 1599

Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val

495 500 505

aat gca gct cca ttc caa tcc atg caa acg gta ttc aat atg aat gcc 1647

Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala

510 515 520

cca gtt cct cct gtt aat gaa cca gaa act tta aaa cag caa aat cag 1695

Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln

525 530 535

tac cag gcc agt tac aac cag agc ttt tcc agt cag cct cac caa gta 1743

Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val

540 545 550

gaa caa aca gag ctt cag caa gaa cag ctt caa aca gtg gtt ggc act 1791

Glu Gln Thr Glu Leu Gln Gln Glu Gln Leu Gln Thr Val Val Gly Thr

555 560 565 570

tat cat ggt tct cag gac cag ccc cat caa gtg act ggt aac cac cag 1839

Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Thr Gly Asn His Gln

575 580 585

cag cct cct cag cag aac act gga ttt cca cgt agc aat cag ccc tat 1887

Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr

590 595 600

tac aac agt cgt ggt gtg tct cgt gga ggt tcc cgt ggt gct aga ggc 1935

Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly

605 610 615

ttg atg aat gga tac aga gga cct gct aat gga ttc aga gga gga tat 1983

Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr

620 625 630

gat ggt tac cgc cct tca ttc tct act aac act cca aac agt ggt tat 2031

Asp Gly Tyr Arg Pro Ser Phe Ser Thr Asn Thr Pro Asn Ser Gly Tyr

635 640 645 650

aca caa tct caa ttc agt gct ccc cgg gac tac tct ggc tat cag cgg 2079

Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg

655 660 665

gat gga tat cag cag aat ttc aag cga ggc tct ggg cag agt gga cca 2127

Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro

670 675 680

cgg gga gcc cca cga ggt cgt gga ggg ccc cca aga ccc aac aga ggg 2175

Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly

685 690 695

atg ccg caa atg aac act cag caa gtg aat taa tctgattcac aggattatgt 2228

Met Pro Gln Met Asn Thr Gln Gln Val Asn

700 705

ttaatcgcca aaaacacact ggccagtgta ccataatatg ttaccagaag agttattatc 2288

tatttgttct ccctttcagg aaacttattg taaagggact gttttcatcc cataaagaca 2348

ggactacaat tgtcagcttt atattacctg gatatggaag gaaactattt ttactctgca 2408

tgttctgtcc taagcgtcat cttgagcctt gcacatgata ctcagattcc tcacccttgc 2468

ttaggagtaa aacataatat actttaatgg ggtgatatct ccatagttat ttgaagtggc 2528

ttggataaag caagactgac ttctgacatt ggataaaatc tacaaatcag ccctagagtc 2588

attcagtggt aactgacaaa actaaaatat ttcccttgaa aggaagatgg aaggagtgga 2648

gtgtggtttg gcagaacaac tgcatttcac agcttttcca cttaaattgg agcactgaac 2708

atttagatgc ataccgaatt atgcatgggc cctaatcaca cagacaaggc tggtgccagc 2768

cttaggcttg acacggcagt gttcaccctc tggccagacg actgtggttc aagacacatg 2828

taaattgctt tttaacagct gatactgtat aagacaaagc caaaatgcaa aattaggctt 2888

tgattggcac ttttcgaaaa atatgcaaca attaagggat ataatctgga tggccgcttc 2948

tgtacttaat gtgaaatatt tagatacctt tcaaacactt aacagtttct ttgacaatga 3008

gttttgtaag gattggtagt aaatatcatt ccttatgacg tacattgtct gtcactaatc 3068

cttggatctt gctgtattgt cacctaaatt ggtacaggta ctgatgaaaa tctaatggat 3128

aatcataaca ctcttggtta catgtttttc ctgcagcctg aaagttttta taagaaaaag 3188

acatcaaatg cctgctgctg ccaccctttt aaattgctat cttttgaaaa gcaccagtat 3248

gtgttttaga ttgatttccc tattttaggg aaatgacagt cagtagtttc acttctgatg 3308

gtataagcaa acaaataaaa catgtttata aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3368

aaaaaaaaaa aaaaaaaa 3386

<210> 16

<211> 708

<212> PRT

<213> 牛(Bos taurus)

<400> 16

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Asn Glu Ala Gly Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Met Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Glu Val Arg Thr Asp Leu Lys Gln Gly Leu Asn Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Ala Ala Glu

260 265 270

Ala Glu Pro Glu Pro Val Glu Glu Tyr Thr Glu Gln Asn Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Asp Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ala Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Ala Gln Asn Met Asp

385 390 395 400

Ile Pro Gln Leu Val Cys Pro Pro Val His Ser Glu Ser Arg Leu Ala

405 410 415

Gln Pro Asn Gln Val Ser Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

Pro Ser His Ala Thr Asp Gln Arg Pro Gln Lys Glu Pro Ile Asp Gln

450 455 460

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn

515 520 525

Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn

530 535 540

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

Gln Glu Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

Gln Pro His Gln Val Thr Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

Thr Gly Phe Pro Arg Ser Asn Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

Phe Ser Thr Asn Thr Pro Asn Ser Gly Tyr Thr Gln Ser Gln Phe Ser

645 650 655

Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn

660 665 670

Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly

675 680 685

Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln Met Asn Thr

690 695 700

Gln Gln Val Asn

705

<210> 17

<211> 3150

<212> DNA

<213> 马(Equus caballus)

<220>

<221> CDS

<222> (1)..(1917)

<400> 17

atg gag ggc aag ctc gat gat tac caa gag cga atg aac aaa gga gaa 48

Met Glu Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu

1 5 10 15

agg ctt aat cag gat cag ctg gat gct gtg tct aag tac cag gaa gtc 96

Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val

20 25 30

aca aat aac ttg gag ttt gcg aaa gaa ttg cag agg agt ttc atg gcg 144

Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala

35 40 45

ttg agt cag gat att cag aaa aca ata aag aag acg gca cgt cgg gag 192

Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu

50 55 60

cag ctt atg aga gaa gaa gct gaa cag aaa cgt tta aaa act gta ctt 240

Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu

65 70 75 80

gag ctg cag tat gtt ttg gac aaa ttg gga gat gaa gaa gtg cga act 288

Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Glu Glu Val Arg Thr

85 90 95

gac ctg aaa caa ggt ttg aat gga gtg cca ata ctc tct gaa gaa gag 336

Asp Leu Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu

100 105 110

ttg tcg ctg ttg gat gag ttc tac aag tta gca gac cct gta cgg gac 384

Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Val Arg Asp

115 120 125

atg agc ttg agg ttg aat gag cag tat gag cat gcc tcc att cac ctg 432

Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu

130 135 140

tgg gac ttg ctg gaa ggg aag gaa aaa tct gtc tgt gga aca acc tat 480

Trp Asp Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr

145 150 155 160

aaa gct ctg agg gaa att gtt gag cgt gtt ttc cag tcc aac tac ttt 528

Lys Ala Leu Arg Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe

165 170 175

gac agc acc cac aac cac cag aat ggg ctc tgt gag gag gaa gag gct 576

Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala

180 185 190

acc tca gct cca aca gct gaa gac cag gga gct gaa gct gaa cct gag 624

Thr Ser Ala Pro Thr Ala Glu Asp Gln Gly Ala Glu Ala Glu Pro Glu

195 200 205

cca gca gaa gaa tac act gaa caa agt gaa gtt gaa tca aca gag tat 672

Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr

210 215 220

gta aat aga cag ttt atg gca gaa gcg cag ttc agt ggt gag aag gag 720

Val Asn Arg Gln Phe Met Ala Glu Ala Gln Phe Ser Gly Glu Lys Glu

225 230 235 240

cag gtg gat gag tgg aca gtc gag acg gtc gag gtg gta aat tca ctc 768

Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu

245 250 255

cag cag caa cct cag gct gca tct cct tca gta ccg gag ccc cac tct 816

Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser

260 265 270

ttg act cca gtg gct cag gca gat ccc ctt gtg aga aga cag cga gta 864

Leu Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val

275 280 285

cag gac ctt atg gcg caa atg cag ggg ccc tat aat ttc ata cag gat 912

Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp

290 295 300

tca atg ctg gat ttt gaa aac cag aca ctt gat cct gcc att gta tct 960

Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser

305 310 315 320

gca cag cct atg aat cca gca cag aat atg gac atg ccc cag ctg gtt 1008

Ala Gln Pro Met Asn Pro Ala Gln Asn Met Asp Met Pro Gln Leu Val

325 330 335

tgc cct cca gtt cat gct gaa tct aga ctt gct caa cct aat caa gtt 1056

Cys Pro Pro Val His Ala Glu Ser Arg Leu Ala Gln Pro Asn Gln Val

340 345 350

cct gta caa cca gaa gct aca cag gtt cct ttg gtt tca tcc aca agt 1104

Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser

355 360 365

gag ggg tat aca gca tct cag ccc ttg tac cag cct tct cat gct aca 1152

Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr

370 375 380

gag caa cga ccg caa aag gaa ccg act gac cag atc cag gca aca atc 1200

Glu Gln Arg Pro Gln Lys Glu Pro Thr Asp Gln Ile Gln Ala Thr Ile

385 390 395 400

tct tta aat aca gac cag act aca gca tca tca tcc ctt cct gct gct 1248

Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala

405 410 415

tct cag cct cag gtg ttc cag gct ggg aca agc aaa cct tta cac agc 1296

Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser

420 425 430

agt ggg atc aat gta aat gca gcg cca ttc cag tcc atg caa acg gtg 1344

Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val

435 440 445

ttc aac atg aat gcc ccg gtt cct cct gtt aat gaa cca gaa act tta 1392

Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu

450 455 460

aaa cag caa aat cag tac cag gcc agc tat aac cag agc ttt tcc agt 1440

Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser

465 470 475 480

ccg cct cac caa gta gag cag aca gag ctt ccg caa gag cag ctt cag 1488

Pro Pro His Gln Val Glu Gln Thr Glu Leu Pro Gln Glu Gln Leu Gln

485 490 495

acg gtg gtt ggt act tac cat gct tcc caa gac cag ccc cat caa gtg 1536

Thr Val Val Gly Thr Tyr His Ala Ser Gln Asp Gln Pro His Gln Val

500 505 510

acc ggt aac cac cag cag cct ccc cag cag aac act ggg ttt cca cgt 1584

Thr Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg

515 520 525

agc agt cag ccc tat tac aac agt cgt ggt gtg tct cgt gga ggc tcc 1632

Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser

530 535 540

cgt ggt gct aga ggc ttg atg aat gga tac agg ggc cct gcc aat gga 1680

Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly

545 550 555 560

ttc aga gga gga tat gat ggt tac cgc cct tcg ttc tct aac act cca 1728

Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro

565 570 575

aac agc ggt tac aca cag tct cag ttc agt gct ccc cgg gac tac tct 1776

Asn Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser

580 585 590

ggc tat cag cgg gat gga tat cag cag aat ttc aag cga ggc tct ggg 1824

Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly

595 600 605

cag agt gga ccc cgg gga gcc cca cga ggt cgt gga ggg ccc cca aga 1872

Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg

610 615 620

ccc aac aga ggg atg ccg caa atg aac act cag caa gtg aat taa 1917

Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

625 630 635

tctgattcac aggattatct ttaatcgcca aaacacactg gccagtgtac cataatatgt 1977

taccagaaga gttattatct atttgttctc cctttcagga aacttattgt aaagggactg 2037

ttttcatccc ataaagacag gactacagtt gtcagcttta tattacctgg atatggaagg 2097

aaactatttt tactctgcat gttctgtcct aagcgtcatc ttgagccttg cacatgatac 2157

tcagattcct ttcccttgct taggagtaaa acataatata ctttatgggg tgataatatc 2217

tccatagtta tttgaagtgg cttggaaaaa gcaagattga cttttgacat tggataaaat 2277

ctacaaatca gccctagagt ttcatggtca ttcacaaaac taaaatattt cccttgaaag 2337

gaagatggaa ggactggagt gtggtttggc agaacaactg catttcacag cttttcctat 2397

taaattggag cactgaatgt taaatgcata ccaaattatg catgggccct taatcacaca 2457

tacatggcta ccagctttga cacagcacta ttcatcctct ggccaaacga ctgtggttaa 2517

aaacacgtgt aaattgcttt ttaacagctg atactgtaaa agacaaagct aaaatgcaaa 2577

attaggcttt cattggcact tttcgaaaaa tatgcaacaa atttgggatg taatctggat 2637

ggccacttct gtacttaatg tgaagtattt agataccttt ttgaacactt aacagtttct 2697

tcgacaatga cttttgtaag gattggtagt atatatcatt ccttatgaca tacattgtct 2757

gttgctaatc cttggatctt gctgtattgt cacctaaatt ggtacaggta ctgatgaaaa 2817

tctctcatgg ataaacctaa cactcttcgt cacatgtttt tcctgcagcc tgaaggtttt 2877

taaaaggaaa agatatcaaa tgcctgctgc taccaccctt ttaaattgct atcttttgaa 2937

aagcaccagt atgtgttttt agattgattt ccctatttta gggaaatgac agtcagtagt 2997

ttcagttctg atggtataag caaagcaaat aaaacgtgtt tataaaagtt gtatcttgaa 3057

acactggtgt tcaacagcta gcagcttctg tggttcaccc cctgccttgt tagtgttacc 3117

catttatggt tatctccagc agcaatttct cta 3150

<210> 18

<211> 638

<212> PRT

<213> 马(Equus caballus)

<400> 18

Met Glu Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu

1 5 10 15

Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val

20 25 30

Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala

35 40 45

Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu

50 55 60

Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu

65 70 75 80

Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Glu Glu Val Arg Thr

85 90 95

Asp Leu Lys Gln Gly Leu Asn Gly Val Pro Ile Leu Ser Glu Glu Glu

100 105 110

Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Ala Asp Pro Val Arg Asp

115 120 125

Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala Ser Ile His Leu

130 135 140

Trp Asp Leu Leu Glu Gly Lys Glu Lys Ser Val Cys Gly Thr Thr Tyr

145 150 155 160

Lys Ala Leu Arg Glu Ile Val Glu Arg Val Phe Gln Ser Asn Tyr Phe

165 170 175

Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala

180 185 190

Thr Ser Ala Pro Thr Ala Glu Asp Gln Gly Ala Glu Ala Glu Pro Glu

195 200 205

Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr

210 215 220

Val Asn Arg Gln Phe Met Ala Glu Ala Gln Phe Ser Gly Glu Lys Glu

225 230 235 240

Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu

245 250 255

Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser

260 265 270

Leu Thr Pro Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val

275 280 285

Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp

290 295 300

Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser

305 310 315 320

Ala Gln Pro Met Asn Pro Ala Gln Asn Met Asp Met Pro Gln Leu Val

325 330 335

Cys Pro Pro Val His Ala Glu Ser Arg Leu Ala Gln Pro Asn Gln Val

340 345 350

Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser

355 360 365

Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr

370 375 380

Glu Gln Arg Pro Gln Lys Glu Pro Thr Asp Gln Ile Gln Ala Thr Ile

385 390 395 400

Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala

405 410 415

Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser

420 425 430

Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val

435 440 445

Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr Leu

450 455 460

Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Ser

465 470 475 480

Pro Pro His Gln Val Glu Gln Thr Glu Leu Pro Gln Glu Gln Leu Gln

485 490 495

Thr Val Val Gly Thr Tyr His Ala Ser Gln Asp Gln Pro His Gln Val

500 505 510

Thr Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg

515 520 525

Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser

530 535 540

Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly

545 550 555 560

Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro

565 570 575

Asn Ser Gly Tyr Thr Gln Ser Gln Phe Ser Ala Pro Arg Asp Tyr Ser

580 585 590

Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly

595 600 605

Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg

610 615 620

Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

625 630 635

<210> 19

<211> 6181

<212> DNA

<213> 小鼠(Mus musculus)

<220>

<221> CDS

<222> (179)..(2302)

<400> 19

gctggctggc taagtccctc ccgcgccggc tcttgtccca ctaggagcag ctcagagccg 60

cggggacagg gcgaagcggc ctgcgcccac ggagcgcacg tctctgttct caacgcagca 120

ccacccttgc ccccctcggc tgcccactcc agacgtccag cggctccgcg cgcgcacg 178

atg ccc tcg gcc acc agc cac agc gga agc ggc agc aaa tcg tcg gga 226

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccg ccg tcc ggt tcc tcc ggg agt gag gcg gcg gcc ggg gca 274

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

gct gcg ccg gct tct cag cat ccg gca acc ggc acc ggc gcc gtc cag 322

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

acc gag gcc atg aag cag att ctc ggc gta atc gac aag aaa ctt cgg 370

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

aac ctg gag aag aaa aag ggt aaa ctt gat gat tac cag gaa cga atg 418

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

aat aaa ggg gaa agg ctc aat caa gac cag ctg gat gcc gta tct aag 466

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

tac cag gaa gtc aca aat aat ttg gag ttt gca aag gaa tta cag agg 514

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

agt ttc atg gca tta agt caa gat att cag aaa aca ata aag aag aca 562

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

gca cgt cgg gaa cag ctt atg aga gaa gaa gca gaa cag aag cgc tta 610

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

aaa act gta ctt gag tta cag tat gta ttg gat aag ctg gga gat gat 658

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

gat gtg aga aca gat ctg aaa caa ggt ttg agt gga gtg cca ata ttg 706

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

tct gag gag gag ttg tca ttg ctg gat gag ttc tac aag ctc gta gat 754

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

cct gag cgt gac atg agt tta agg tta aat gag cag tat gaa cat gcc 802

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

tca att cac ttg tgg gat ttg ctg gaa ggg aaa gaa aag cct gtg tgt 850

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

gga aca acc tat aaa gct cta aag gaa att gtt gag cgt gtt ttc cag 898

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

tca aac tac ttt gat agc act cac aat cat caa aat ggg ttg tgt gag 946

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

gag gaa gag gcg gct tca gcg ccc aca gtg gag gac cag gta gct gaa 994

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

gct gaa cct gag cca gcg gaa gaa tac aca gag caa agt gag gtt gaa 1042

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

tca aca gag tat gtc aat agg cag ttc atg gca gaa aca cag ttc agc 1090

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

agt ggt gag aag gag caa gtg gat gag tgg aca gtt gaa aca gtt gag 1138

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

gtt gta aac tca ctc cag cag caa cct cag gct gcg tcc cct tca gtc 1186

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

cca gag ccc cac tct ttg act cca gtg gct cag tca gat cca ctt gtg 1234

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

aga agg cag cgt gta caa gat ctt atg gca caa atg caa ggg ccc tat 1282

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

aat ttc ata cag gat tca atg ttg gat ttt gaa aat cag acg ctt gat 1330

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

cct gcc att gta tcc gca cag cct atg aac cct acc cag aac atg gat 1378

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

atg cct cag ctg gtt tgc cct cag gtt cat tct gaa tct aga ctt gcc 1426

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

caa tct aat caa gtt cct gta caa cca gaa gcc aca cag gtt cct ttg 1474

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

gtt tca tcc aca agt gag ggg tat aca gca tct cag ccc ttg tac cag 1522

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

cca tct cat gct acg gag cag cgg ccg cag aaa gag cca atg gat cag 1570

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

att cag gca aca ata tct ttg aat aca gac cag act aca gca tcc tca 1618

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

tcc ctt cct gct gct tct cag cct caa gtg ttc cag gct ggg aca agt 1666

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

aaa cct ttg cac agc agt gga atc aat gta aat gca gct cca ttc cag 1714

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

tcc atg caa acg gtg ttc aat atg aat gct cca gtc cct cct gct aat 1762

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

gaa cca gaa acg tta aaa caa cag agt cag tac cag gcc act tat aac 1810

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

cag agt ttt tcc agt cag cct cac caa gtg gaa caa aca gag ctt caa 1858

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

caa gac caa ctg caa acg gtg gtt ggc act tac cat gga tcc cag gac 1906

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

cag cct cat caa gtg cct ggt aac cac cag caa ccc cca cag cag aac 1954

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

act ggc ttt cca cgt agc agt cag cct tat tac aac agt cgt ggg gta 2002

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

tct cga gga ggg tct cgt ggt gcc aga ggc ttg atg aat gga tac agg 2050

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

ggc cct gcc aat gga ttt aga gga gga tat gat ggt tac cgc cct tca 2098

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

ttc tcg aac act cca aac agt ggt tat tca cag tct cag ttc act gct 2146

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

ccc cgg gac tac tct ggt tac cag cgg gat gga tat cag cag aat ttc 2194

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

aag cga ggc tct ggg cag agt gga cca cgg gga gcc cca cga ggt cgt 2242

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg

675 680 685

gga ggg ccc cca aga ccc aac aga ggg atg ccg caa atg aac act cag 2290

Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln

690 695 700

caa gtg aat taa tgtgatacac aggattatgt ttaatcgcca aaaacacact 2342

Gln Val Asn

705

ggccagtgta ccataatatg ttaccagaag agttattatc tatttgttct ccctttcagg 2402

aaacttattg taaagggact gttttcatcc cataaagaca ggactgcaat tgtcagcttt 2462

acattacctg gatatggaag gaaactattt ttattctgca tgttctgtcc taagcgtcat 2522

cttgagcctt gcacacaata caatactcag attcctcacc cttgcttagg agtaaaacat 2582

tatatactta tggggtgata atatctccat agttagttga agtggcttgg aaaaaaaatg 2642

caagattgaa tttttgacct tggataaaat ctacaatcag ccctagaact attcagtggt 2702

aattgacaaa gttaaagcat tttctttgaa aggaagatgg aaggagtgga gtgtggttta 2762

gcaaaactgc atttcatagc tttcccatta aattggagca ccgacagatt aaaagcatac 2822

caaattatgc atgggtcctt actcacacaa gtgaggctgg ctaccagcct tgacatagca 2882

ctcactagtc ttctggccaa acgactgtga ttaaaacaca tgtaaattgc tctttagtag 2942

tggatactgt gtaagacaaa gccaaattgc aaatcaggct ttgattggct cttctggaaa 3002

atatgcatca aatatggggg ataatctgga tgggctgctg ctgtgctcaa tgtgaactat 3062

ttagatacct ttggaacact taacagtttc tctgaacaat gacttacatg gggattggtc 3122

ctgtttgtca ttcctcacca taattgcatt gtcatcacta atccttggat cttgctgtat 3182

tgttactcaa attggtaata ggtactgatg gaaatcgcta atggatggat aatcataaca 3242

cttttggtca catgttttct cctgcagcct gaaagttctt aaagaaaaag atatcaaatg 3302

cctgctgcta ccaccctttt aaattgctat ctttagaaaa gcaccggtat gtgttttaga 3362

ttcatttccc tgttttaggg aaatgacagg cagtagtttc agttctgatg gcaaaacaaa 3422

taaaaacatg tttctaaaag ttgtatcttg aaacactggt gttcaacagc tagcagctaa 3482

agtaattcaa cccatgcatt gctagtgtca cagcctttgg ttatgtctag tagctgtttc 3542

tgaagtattt tcatttatct tttgtcaaat ttaaccctgt ttgaattctc tcctttcctc 3602

aaggagacac ttatgttcaa agtgttgatt ctttgcctta ggtgcataga gagtagacag 3662

tttggagatg gaaaggttag cagtgactta gccatatgtt ctgtgttgga atttgtgcta 3722

gcagtttgag cactagctct gcgtgcctat gaactgaatg ctgcttgtcc cattccattt 3782

tatgtcatgg agaaataatt ccacttggta acacaaaggc taagttaatg ttattttctg 3842

tacagaaatt aaattttact tttagccttt tgtaaacttt tttttttttt ttccaagccg 3902

gtatcagcta ctcaaaacaa ttctcagata ttcatcatta gacaactgga gtttttgctg 3962

gttttgtagc ctactaaaac tgctgaggct gttgaacatt ccacattcaa aagttttgta 4022

gggtggtgga taatggggaa gcttcaatgt ttattttaaa ataaataaaa taagttcttg 4082

acttttctca tgtgtggtta tggtacatca tattggaagg gttatctgtt tacttttgcc 4142

aagactattt tgccagcacc tacacttgtg tgctttaaaa gacaactacc tgggatgtac 4202

cacaaccata tgttaattgt attttattgg gatggataaa atgtttgtgg tttattggat 4262

aatccctaga tggtgtgtta cgtgtgtaga atataatttt atgatagtaa gaaagcaaaa 4322

ttgaagaaaa taagtttagt attgaatttg agttctgaag tgaattcagg gaatgtctca 4382

cgtttcgggc ttctacccaa agtgtagggc agaaggtgta aaagttgttt gtagtttgac 4442

ttgtttattt tttaagttgc ttattccttt caacagcaac atatcattag ctgtcattct 4502

accattgcag ttctagtgag ttttaacgtc tgcattcaag actgttttaa aagcaacctc 4562

actggacaga gaactgctaa agtcttttcc ttaagatctg agtctttgtt actcagtatc 4622

ttctataata tgcaaatgct tgtctagagg cagaagacct tttgtttggt caagtgtgta 4682

ttttaccaga gtacagggaa ctgatggtcc tacatgtctc ttagtgtagt aagactataa 4742

aatcttttgt acatgcacaa ttcacagtat gtttagatac cacgtgtata atgccccccc 4802

ctcccccagg tagcatgcca ttgatgactt tttgcttagg gccattttat taccagggcc 4862

ttaatattcc taaaaagatg attttttttc atcctttctc ctcttttgat cattgtatct 4922

tgatattaaa aacatgacct tccaatgatt gtagtaaatt aacttctata gttcttttgt 4982

ctctatatgt attcatatat atgctattgt atagagactt caaggagaca tggagatgca 5042

tgcttattct caggttcatt cactaaggtg cttggcagac aaccagtttc taagtgcaga 5102

atgtagttaa gcagcttcat atatgtgcca ggcaatttgt tttgttaaat tttcatctac 5162

ttaaggaaat agggtattgt agcttaggct gatcataccc ttcatttcaa ccttaagctc 5222

tcaacctgca tccatccgac ttgagctatt aagtacttta gttttatcga gtataagtta 5282

acagaaaaag taaattaagc tttgccttta ctattttgaa tttatataca ttctggaaaa 5342

acttagaaac tgttgtatat ttcattagat taaattatat gaaaatgtga ttgtttatag 5402

caaagcctgt gagttgcata caccctaagg aaaactcctt aagtgctcct tgaagagaga 5462

agaaacaatt ctgggtctgg tctttttaag aacaaagcta gactactgta tgttagcact 5522

gtacattaat agtctgttgt gaagcttgag cagtttcctg catagccttg atccttcacc 5582

gttggcattg aaaatagcag tatccctgat gtacttaaaa cttaaagtca ggttttggta 5642

tatttatttg taagtcttaa tttcctctaa atactatatc tctttagcga gacaacctga 5702

aatttattag cacatttggg tatctcttgc ttggcattat ggccagtgtt aactattcag 5762

tggtgaaaaa attacccctc aagacactgg agtgacccca gatgtgtgta gtaagtggca 5822

tggttcaact gtgtggttaa tgataaatat atgacttagt cggtatgatc tggaaagact 5882

tgattgaaag ataattcagc tgacataagg atgagtgagg agtggcaaac tggataaaag 5942

agtcaagaga cctgtattcc agtgactcct gttttgttta agcattagca agatctgtct 6002

ggggaaactg gatagggcag ttttcttcca tgtttagttt ttgtctcaac atttggaagc 6062

tattgaaggt tttaaaatgg tgtgtattgt ttttttttgg ggggggggtg gccagaatag 6122

tgggtcatct aataaaactg ccatttaaaa gatcaaaaaa aaaaaaaaaa aaaaaaaaa 6181

<210> 20

<211> 707

<212> PRT

<213> 小鼠(Mus musculus)

<400> 20

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg

675 680 685

Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln

690 695 700

Gln Val Asn

705

<210> 21

<211> 6141

<212> DNA

<213> 小鼠(Mus musculus)

<220>

<221> CDS

<222> (139)..(2262)

<400> 21

cccaccgcgc gcgcgcgtag ccgcctgccc gcccgcccgc tgcgcgtttt gtcccgcgtc 60

tctccccgtc cgtctcctga cttgctggtc ttgtccttcc ctcccgcttt tttcctctcc 120

tctcttctcg gtctaaag atg ccc tcg gcc acc agc cac agc gga agc ggc 171

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly

1 5 10

agc aaa tcg tcg gga ccg ccg ccg ccg tcc ggt tcc tcc ggg agt gag 219

Ser Lys Ser Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu

15 20 25

gcg gcg gcc ggg gca gct gcg ccg gct tct cag cat ccg gca acc ggc 267

Ala Ala Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly

30 35 40

acc ggc gcc gtc cag acc gag gcc atg aag cag att ctc ggc gta atc 315

Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile

45 50 55

gac aag aaa ctt cgg aac ctg gag aag aaa aag ggt aaa ctt gat gat 363

Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp

60 65 70 75

tac cag gaa cga atg aat aaa ggg gaa agg ctc aat caa gac cag ctg 411

Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu

80 85 90

gat gcc gta tct aag tac cag gaa gtc aca aat aat ttg gag ttt gca 459

Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala

95 100 105

aag gaa tta cag agg agt ttc atg gca tta agt caa gat att cag aaa 507

Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys

110 115 120

aca ata aag aag aca gca cgt cgg gaa cag ctt atg aga gaa gaa gca 555

Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala

125 130 135

gaa cag aag cgc tta aaa act gta ctt gag tta cag tat gta ttg gat 603

Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp

140 145 150 155

aag ctg gga gat gat gat gtg aga aca gat ctg aaa caa ggt ttg agt 651

Lys Leu Gly Asp Asp Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser

160 165 170

gga gtg cca ata ttg tct gag gag gag ttg tca ttg ctg gat gag ttc 699

Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe

175 180 185

tac aag ctc gta gat cct gag cgt gac atg agt tta agg tta aat gag 747

Tyr Lys Leu Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu

190 195 200

cag tat gaa cat gcc tca att cac ttg tgg gat ttg ctg gaa ggg aaa 795

Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys

205 210 215

gaa aag cct gtg tgt gga aca acc tat aaa gct cta aag gaa att gtt 843

Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val

220 225 230 235

gag cgt gtt ttc cag tca aac tac ttt gat agc act cac aat cat caa 891

Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln

240 245 250

aat ggg ttg tgt gag gag gaa gag gcg gct tca gcg ccc aca gtg gag 939

Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu

255 260 265

gac cag gta gct gaa gct gaa cct gag cca gcg gaa gaa tac aca gag 987

Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu

270 275 280

caa agt gag gtt gaa tca aca gag tat gtc aat agg cag ttc atg gca 1035

Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala

285 290 295

gaa aca cag ttc agc agt ggt gag aag gag caa gtg gat gag tgg aca 1083

Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr

300 305 310 315

gtt gaa aca gtt gag gtt gta aac tca ctc cag cag caa cct cag gct 1131

Val Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala

320 325 330

gcg tcc cct tca gtc cca gag ccc cac tct ttg act cca gtg gct cag 1179

Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln

335 340 345

tca gat cca ctt gtg aga agg cag cgt gta caa gat ctt atg gca caa 1227

Ser Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln

350 355 360

atg caa ggg ccc tat aat ttc ata cag gat tca atg ttg gat ttt gaa 1275

Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu

365 370 375

aat cag acg ctt gat cct gcc att gta tcc gca cag cct atg aac cct 1323

Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro

380 385 390 395

acc cag aac atg gat atg cct cag ctg gtt tgc cct cag gtt cat tct 1371

Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys Pro Gln Val His Ser

400 405 410

gaa tct aga ctt gcc caa tct aat caa gtt cct gta caa cca gaa gcc 1419

Glu Ser Arg Leu Ala Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala

415 420 425

aca cag gtt cct ttg gtt tca tcc aca agt gag ggg tat aca gca tct 1467

Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser

430 435 440

cag ccc ttg tac cag cca tct cat gct acg gag cag cgg ccg cag aaa 1515

Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys

445 450 455

gag cca atg gat cag att cag gca aca ata tct ttg aat aca gac cag 1563

Glu Pro Met Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln

460 465 470 475

act aca gca tcc tca tcc ctt cct gct gct tct cag cct caa gtg ttc 1611

Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe

480 485 490

cag gct ggg aca agt aaa cct ttg cac agc agt gga atc aat gta aat 1659

Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn

495 500 505

gca gct cca ttc cag tcc atg caa acg gtg ttc aat atg aat gct cca 1707

Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro

510 515 520

gtc cct cct gct aat gaa cca gaa acg tta aaa caa cag agt cag tac 1755

Val Pro Pro Ala Asn Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr

525 530 535

cag gcc act tat aac cag agt ttt tcc agt cag cct cac caa gtg gaa 1803

Gln Ala Thr Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu

540 545 550 555

caa aca gag ctt caa caa gac caa ctg caa acg gtg gtt ggc act tac 1851

Gln Thr Glu Leu Gln Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr

560 565 570

cat gga tcc cag gac cag cct cat caa gtg cct ggt aac cac cag caa 1899

His Gly Ser Gln Asp Gln Pro His Gln Val Pro Gly Asn His Gln Gln

575 580 585

ccc cca cag cag aac act ggc ttt cca cgt agc agt cag cct tat tac 1947

Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr

590 595 600

aac agt cgt ggg gta tct cga gga ggg tct cgt ggt gcc aga ggc ttg 1995

Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu

605 610 615

atg aat gga tac agg ggc cct gcc aat gga ttt aga gga gga tat gat 2043

Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp

620 625 630 635

ggt tac cgc cct tca ttc tcg aac act cca aac agt ggt tat tca cag 2091

Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln

640 645 650

tct cag ttc act gct ccc cgg gac tac tct ggt tac cag cgg gat gga 2139

Ser Gln Phe Thr Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly

655 660 665

tat cag cag aat ttc aag cga ggc tct ggg cag agt gga cca cgg gga 2187

Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly

670 675 680

gcc cca cga ggt cgt gga ggg ccc cca aga ccc aac aga ggg atg ccg 2235

Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro

685 690 695

caa atg aac act cag caa gtg aat taa tgtgatacac aggattatgt 2282

Gln Met Asn Thr Gln Gln Val Asn

700 705

ttaatcgcca aaaacacact ggccagtgta ccataatatg ttaccagaag agttattatc 2342

tatttgttct ccctttcagg aaacttattg taaagggact gttttcatcc cataaagaca 2402

ggactgcaat tgtcagcttt acattacctg gatatggaag gaaactattt ttattctgca 2462

tgttctgtcc taagcgtcat cttgagcctt gcacacaata caatactcag attcctcacc 2522

cttgcttagg agtaaaacat tatatactta tggggtgata atatctccat agttagttga 2582

agtggcttgg aaaaaaaatg caagattgaa tttttgacct tggataaaat ctacaatcag 2642

ccctagaact attcagtggt aattgacaaa gttaaagcat tttctttgaa aggaagatgg 2702

aaggagtgga gtgtggttta gcaaaactgc atttcatagc tttcccatta aattggagca 2762

ccgacagatt aaaagcatac caaattatgc atgggtcctt actcacacaa gtgaggctgg 2822

ctaccagcct tgacatagca ctcactagtc ttctggccaa acgactgtga ttaaaacaca 2882

tgtaaattgc tctttagtag tggatactgt gtaagacaaa gccaaattgc aaatcaggct 2942

ttgattggct cttctggaaa atatgcatca aatatggggg ataatctgga tgggctgctg 3002

ctgtgctcaa tgtgaactat ttagatacct ttggaacact taacagtttc tctgaacaat 3062

gacttacatg gggattggtc ctgtttgtca ttcctcacca taattgcatt gtcatcacta 3122

atccttggat cttgctgtat tgttactcaa attggtaata ggtactgatg gaaatcgcta 3182

atggatggat aatcataaca cttttggtca catgttttct cctgcagcct gaaagttctt 3242

aaagaaaaag atatcaaatg cctgctgcta ccaccctttt aaattgctat ctttagaaaa 3302

gcaccggtat gtgttttaga ttcatttccc tgttttaggg aaatgacagg cagtagtttc 3362

agttctgatg gcaaaacaaa taaaaacatg tttctaaaag ttgtatcttg aaacactggt 3422

gttcaacagc tagcagctaa agtaattcaa cccatgcatt gctagtgtca cagcctttgg 3482

ttatgtctag tagctgtttc tgaagtattt tcatttatct tttgtcaaat ttaaccctgt 3542

ttgaattctc tcctttcctc aaggagacac ttatgttcaa agtgttgatt ctttgcctta 3602

ggtgcataga gagtagacag tttggagatg gaaaggttag cagtgactta gccatatgtt 3662

ctgtgttgga atttgtgcta gcagtttgag cactagctct gcgtgcctat gaactgaatg 3722

ctgcttgtcc cattccattt tatgtcatgg agaaataatt ccacttggta acacaaaggc 3782

taagttaatg ttattttctg tacagaaatt aaattttact tttagccttt tgtaaacttt 3842

tttttttttt ttccaagccg gtatcagcta ctcaaaacaa ttctcagata ttcatcatta 3902

gacaactgga gtttttgctg gttttgtagc ctactaaaac tgctgaggct gttgaacatt 3962

ccacattcaa aagttttgta gggtggtgga taatggggaa gcttcaatgt ttattttaaa 4022

ataaataaaa taagttcttg acttttctca tgtgtggtta tggtacatca tattggaagg 4082

gttatctgtt tacttttgcc aagactattt tgccagcacc tacacttgtg tgctttaaaa 4142

gacaactacc tgggatgtac cacaaccata tgttaattgt attttattgg gatggataaa 4202

atgtttgtgg tttattggat aatccctaga tggtgtgtta cgtgtgtaga atataatttt 4262

atgatagtaa gaaagcaaaa ttgaagaaaa taagtttagt attgaatttg agttctgaag 4322

tgaattcagg gaatgtctca cgtttcgggc ttctacccaa agtgtagggc agaaggtgta 4382

aaagttgttt gtagtttgac ttgtttattt tttaagttgc ttattccttt caacagcaac 4442

atatcattag ctgtcattct accattgcag ttctagtgag ttttaacgtc tgcattcaag 4502

actgttttaa aagcaacctc actggacaga gaactgctaa agtcttttcc ttaagatctg 4562

agtctttgtt actcagtatc ttctataata tgcaaatgct tgtctagagg cagaagacct 4622

tttgtttggt caagtgtgta ttttaccaga gtacagggaa ctgatggtcc tacatgtctc 4682

ttagtgtagt aagactataa aatcttttgt acatgcacaa ttcacagtat gtttagatac 4742

cacgtgtata atgccccccc ctcccccagg tagcatgcca ttgatgactt tttgcttagg 4802

gccattttat taccagggcc ttaatattcc taaaaagatg attttttttc atcctttctc 4862

ctcttttgat cattgtatct tgatattaaa aacatgacct tccaatgatt gtagtaaatt 4922

aacttctata gttcttttgt ctctatatgt attcatatat atgctattgt atagagactt 4982

caaggagaca tggagatgca tgcttattct caggttcatt cactaaggtg cttggcagac 5042

aaccagtttc taagtgcaga atgtagttaa gcagcttcat atatgtgcca ggcaatttgt 5102

tttgttaaat tttcatctac ttaaggaaat agggtattgt agcttaggct gatcataccc 5162

ttcatttcaa ccttaagctc tcaacctgca tccatccgac ttgagctatt aagtacttta 5222

gttttatcga gtataagtta acagaaaaag taaattaagc tttgccttta ctattttgaa 5282

tttatataca ttctggaaaa acttagaaac tgttgtatat ttcattagat taaattatat 5342

gaaaatgtga ttgtttatag caaagcctgt gagttgcata caccctaagg aaaactcctt 5402

aagtgctcct tgaagagaga agaaacaatt ctgggtctgg tctttttaag aacaaagcta 5462

gactactgta tgttagcact gtacattaat agtctgttgt gaagcttgag cagtttcctg 5522

catagccttg atccttcacc gttggcattg aaaatagcag tatccctgat gtacttaaaa 5582

cttaaagtca ggttttggta tatttatttg taagtcttaa tttcctctaa atactatatc 5642

tctttagcga gacaacctga aatttattag cacatttggg tatctcttgc ttggcattat 5702

ggccagtgtt aactattcag tggtgaaaaa attacccctc aagacactgg agtgacccca 5762

gatgtgtgta gtaagtggca tggttcaact gtgtggttaa tgataaatat atgacttagt 5822

cggtatgatc tggaaagact tgattgaaag ataattcagc tgacataagg atgagtgagg 5882

agtggcaaac tggataaaag agtcaagaga cctgtattcc agtgactcct gttttgttta 5942

agcattagca agatctgtct ggggaaactg gatagggcag ttttcttcca tgtttagttt 6002

ttgtctcaac atttggaagc tattgaaggt tttaaaatgg tgtgtattgt ttttttttgg 6062

ggggggggtg gccagaatag tgggtcatct aataaaactg ccatttaaaa gatcaaaaaa 6122

aaaaaaaaaa aaaaaaaaa 6141

<210> 22

<211> 707

<212> PRT

<213> 小鼠(Mus musculus)

<400> 22

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg

675 680 685

Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln

690 695 700

Gln Val Asn

705

<210> 23

<211> 6114

<212> DNA

<213> 小鼠(Mus musculus)

<220>

<221> CDS

<222> (139)..(2235)

<400> 23

cccaccgcgc gcgcgcgtag ccgcctgccc gcccgcccgc tgcgcgtttt gtcccgcgtc 60

tctccccgtc cgtctcctga cttgctggtc ttgtccttcc ctcccgcttt tttcctctcc 120

tctcttctcg gtctaaag atg ccc tcg gcc acc agc cac agc gga agc ggc 171

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly

1 5 10

agc aaa tcg tcg gga ccg ccg ccg ccg tcc ggt tcc tcc ggg agt gag 219

Ser Lys Ser Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu

15 20 25

gcg gcg gcc ggg gca gct gcg ccg gct tct cag cat ccg gca acc ggc 267

Ala Ala Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly

30 35 40

acc ggc gcc gtc cag acc gag gcc atg aag cag att ctc ggc gta atc 315

Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile

45 50 55

gac aag aaa ctt cgg aac ctg gag aag aaa aag ggt aaa ctt gat gat 363

Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp

60 65 70 75

tac cag gaa cga atg aat aaa ggg gaa agg ctc aat caa gac cag ctg 411

Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu

80 85 90

gat gcc gta tct aag tac cag gaa gtc aca aat aat ttg gag ttt gca 459

Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala

95 100 105

aag gaa tta cag agg agt ttc atg gca tta agt caa gat att cag aaa 507

Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys

110 115 120

aca ata aag aag aca gca cgt cgg gaa cag ctt atg aga gaa gaa gca 555

Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala

125 130 135

gaa cag aag cgc tta aaa act gta ctt gag tta cag tat gta ttg gat 603

Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp

140 145 150 155

aag ctg gga gat gat gat gtg aga aca gat ctg aaa caa ggt ttg agt 651

Lys Leu Gly Asp Asp Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser

160 165 170

gga gtg cca ata ttg tct gag gag gag ttg tca ttg ctg gat gag ttc 699

Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe

175 180 185

tac aag ctc gta gat cct gag cgt gac atg agt tta agg tta aat gag 747

Tyr Lys Leu Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu

190 195 200

cag tat gaa cat gcc tca att cac ttg tgg gat ttg ctg gaa ggg aaa 795

Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys

205 210 215

gaa aag cct gtg tgt gga aca acc tat aaa gct cta aag gaa att gtt 843

Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val

220 225 230 235

gag cgt gtt ttc cag tca aac tac ttt gat agc act cac aat cat caa 891

Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln

240 245 250

aat ggg ttg tgt gag gag gaa gag gcg gct tca gcg ccc aca gtg gag 939

Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu

255 260 265

gac cag gta gct gaa gct gaa cct gag cca gcg gaa gaa tac aca gag 987

Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu

270 275 280

caa agt gag gtt gaa tca aca gag tat gtc aat agg cag ttc atg gca 1035

Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala

285 290 295

gaa aca cag ttc agc agt ggt gag aag gag caa gtg gat gag tgg aca 1083

Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr

300 305 310 315

gtt gaa aca gtt gag gtt gta aac tca ctc cag cag caa cct cag gct 1131

Val Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala

320 325 330

gcg tcc cct tca gtc cca gag ccc cac tct ttg act cca gtg gct cag 1179

Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln

335 340 345

tca gat cca ctt gtg aga agg cag cgt gta caa gat ctt atg gca caa 1227

Ser Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln

350 355 360

atg caa ggg ccc tat aat ttc ata cag acg ctt gat cct gcc att gta 1275

Met Gln Gly Pro Tyr Asn Phe Ile Gln Thr Leu Asp Pro Ala Ile Val

365 370 375

tcc gca cag cct atg aac cct acc cag aac atg gat atg cct cag ctg 1323

Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu

380 385 390 395

gtt tgc cct cag gtt cat tct gaa tct aga ctt gcc caa tct aat caa 1371

Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala Gln Ser Asn Gln

400 405 410

gtt cct gta caa cca gaa gcc aca cag gtt cct ttg gtt tca tcc aca 1419

Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr

415 420 425

agt gag ggg tat aca gca tct cag ccc ttg tac cag cca tct cat gct 1467

Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala

430 435 440

acg gag cag cgg ccg cag aaa gag cca atg gat cag att cag gca aca 1515

Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln Ile Gln Ala Thr

445 450 455

ata tct ttg aat aca gac cag act aca gca tcc tca tcc ctt cct gct 1563

Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala

460 465 470 475

gct tct cag cct caa gtg ttc cag gct ggg aca agt aaa cct ttg cac 1611

Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His

480 485 490

agc agt gga atc aat gta aat gca gct cca ttc cag tcc atg caa acg 1659

Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr

495 500 505

gtg ttc aat atg aat gct cca gtc cct cct gct aat gaa cca gaa acg 1707

Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn Glu Pro Glu Thr

510 515 520

tta aaa caa cag agt cag tac cag gcc act tat aac cag agt ttt tcc 1755

Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn Gln Ser Phe Ser

525 530 535

agt cag cct cac caa gtg gaa caa aca gag ctt caa caa gac caa ctg 1803

Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln Gln Asp Gln Leu

540 545 550 555

caa acg gtg gtt ggc act tac cat gga tcc cag gac cag cct cat caa 1851

Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln

560 565 570

gtg cct ggt aac cac cag caa ccc cca cag cag aac act ggc ttt cca 1899

Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro

575 580 585

cgt agc agt cag cct tat tac aac agt cgt ggg gta tct cga gga ggg 1947

Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly

590 595 600

tct cgt ggt gcc aga ggc ttg atg aat gga tac agg ggc cct gcc aat 1995

Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn

605 610 615

gga ttt aga gga gga tat gat ggt tac cgc cct tca ttc tcg aac act 2043

Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr

620 625 630 635

cca aac agt ggt tat tca cag tct cag ttc act gct ccc cgg gac tac 2091

Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala Pro Arg Asp Tyr

640 645 650

tct ggt tac cag cgg gat gga tat cag cag aat ttc aag cga ggc tct 2139

Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser

655 660 665

ggg cag agt gga cca cgg gga gcc cca cga ggt cgt gga ggg ccc cca 2187

Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro

670 675 680

aga ccc aac aga ggg atg ccg caa atg aac act cag caa gtg aat taa 2235

Arg Pro Asn Arg Gly Met Pro Gln Met Asn Thr Gln Gln Val Asn

685 690 695

tgtgatacac aggattatgt ttaatcgcca aaaacacact ggccagtgta ccataatatg 2295

ttaccagaag agttattatc tatttgttct ccctttcagg aaacttattg taaagggact 2355

gttttcatcc cataaagaca ggactgcaat tgtcagcttt acattacctg gatatggaag 2415

gaaactattt ttattctgca tgttctgtcc taagcgtcat cttgagcctt gcacacaata 2475

caatactcag attcctcacc cttgcttagg agtaaaacat tatatactta tggggtgata 2535

atatctccat agttagttga agtggcttgg aaaaaaaatg caagattgaa tttttgacct 2595

tggataaaat ctacaatcag ccctagaact attcagtggt aattgacaaa gttaaagcat 2655

tttctttgaa aggaagatgg aaggagtgga gtgtggttta gcaaaactgc atttcatagc 2715

tttcccatta aattggagca ccgacagatt aaaagcatac caaattatgc atgggtcctt 2775

actcacacaa gtgaggctgg ctaccagcct tgacatagca ctcactagtc ttctggccaa 2835

acgactgtga ttaaaacaca tgtaaattgc tctttagtag tggatactgt gtaagacaaa 2895

gccaaattgc aaatcaggct ttgattggct cttctggaaa atatgcatca aatatggggg 2955

ataatctgga tgggctgctg ctgtgctcaa tgtgaactat ttagatacct ttggaacact 3015

taacagtttc tctgaacaat gacttacatg gggattggtc ctgtttgtca ttcctcacca 3075

taattgcatt gtcatcacta atccttggat cttgctgtat tgttactcaa attggtaata 3135

ggtactgatg gaaatcgcta atggatggat aatcataaca cttttggtca catgttttct 3195

cctgcagcct gaaagttctt aaagaaaaag atatcaaatg cctgctgcta ccaccctttt 3255

aaattgctat ctttagaaaa gcaccggtat gtgttttaga ttcatttccc tgttttaggg 3315

aaatgacagg cagtagtttc agttctgatg gcaaaacaaa taaaaacatg tttctaaaag 3375

ttgtatcttg aaacactggt gttcaacagc tagcagctaa agtaattcaa cccatgcatt 3435

gctagtgtca cagcctttgg ttatgtctag tagctgtttc tgaagtattt tcatttatct 3495

tttgtcaaat ttaaccctgt ttgaattctc tcctttcctc aaggagacac ttatgttcaa 3555

agtgttgatt ctttgcctta ggtgcataga gagtagacag tttggagatg gaaaggttag 3615

cagtgactta gccatatgtt ctgtgttgga atttgtgcta gcagtttgag cactagctct 3675

gcgtgcctat gaactgaatg ctgcttgtcc cattccattt tatgtcatgg agaaataatt 3735

ccacttggta acacaaaggc taagttaatg ttattttctg tacagaaatt aaattttact 3795

tttagccttt tgtaaacttt tttttttttt ttccaagccg gtatcagcta ctcaaaacaa 3855

ttctcagata ttcatcatta gacaactgga gtttttgctg gttttgtagc ctactaaaac 3915

tgctgaggct gttgaacatt ccacattcaa aagttttgta gggtggtgga taatggggaa 3975

gcttcaatgt ttattttaaa ataaataaaa taagttcttg acttttctca tgtgtggtta 4035

tggtacatca tattggaagg gttatctgtt tacttttgcc aagactattt tgccagcacc 4095

tacacttgtg tgctttaaaa gacaactacc tgggatgtac cacaaccata tgttaattgt 4155

attttattgg gatggataaa atgtttgtgg tttattggat aatccctaga tggtgtgtta 4215

cgtgtgtaga atataatttt atgatagtaa gaaagcaaaa ttgaagaaaa taagtttagt 4275

attgaatttg agttctgaag tgaattcagg gaatgtctca cgtttcgggc ttctacccaa 4335

agtgtagggc agaaggtgta aaagttgttt gtagtttgac ttgtttattt tttaagttgc 4395

ttattccttt caacagcaac atatcattag ctgtcattct accattgcag ttctagtgag 4455

ttttaacgtc tgcattcaag actgttttaa aagcaacctc actggacaga gaactgctaa 4515

agtcttttcc ttaagatctg agtctttgtt actcagtatc ttctataata tgcaaatgct 4575

tgtctagagg cagaagacct tttgtttggt caagtgtgta ttttaccaga gtacagggaa 4635

ctgatggtcc tacatgtctc ttagtgtagt aagactataa aatcttttgt acatgcacaa 4695

ttcacagtat gtttagatac cacgtgtata atgccccccc ctcccccagg tagcatgcca 4755

ttgatgactt tttgcttagg gccattttat taccagggcc ttaatattcc taaaaagatg 4815

attttttttc atcctttctc ctcttttgat cattgtatct tgatattaaa aacatgacct 4875

tccaatgatt gtagtaaatt aacttctata gttcttttgt ctctatatgt attcatatat 4935

atgctattgt atagagactt caaggagaca tggagatgca tgcttattct caggttcatt 4995

cactaaggtg cttggcagac aaccagtttc taagtgcaga atgtagttaa gcagcttcat 5055

atatgtgcca ggcaatttgt tttgttaaat tttcatctac ttaaggaaat agggtattgt 5115

agcttaggct gatcataccc ttcatttcaa ccttaagctc tcaacctgca tccatccgac 5175

ttgagctatt aagtacttta gttttatcga gtataagtta acagaaaaag taaattaagc 5235

tttgccttta ctattttgaa tttatataca ttctggaaaa acttagaaac tgttgtatat 5295

ttcattagat taaattatat gaaaatgtga ttgtttatag caaagcctgt gagttgcata 5355

caccctaagg aaaactcctt aagtgctcct tgaagagaga agaaacaatt ctgggtctgg 5415

tctttttaag aacaaagcta gactactgta tgttagcact gtacattaat agtctgttgt 5475

gaagcttgag cagtttcctg catagccttg atccttcacc gttggcattg aaaatagcag 5535

tatccctgat gtacttaaaa cttaaagtca ggttttggta tatttatttg taagtcttaa 5595

tttcctctaa atactatatc tctttagcga gacaacctga aatttattag cacatttggg 5655

tatctcttgc ttggcattat ggccagtgtt aactattcag tggtgaaaaa attacccctc 5715

aagacactgg agtgacccca gatgtgtgta gtaagtggca tggttcaact gtgtggttaa 5775

tgataaatat atgacttagt cggtatgatc tggaaagact tgattgaaag ataattcagc 5835

tgacataagg atgagtgagg agtggcaaac tggataaaag agtcaagaga cctgtattcc 5895

agtgactcct gttttgttta agcattagca agatctgtct ggggaaactg gatagggcag 5955

ttttcttcca tgtttagttt ttgtctcaac atttggaagc tattgaaggt tttaaaatgg 6015

tgtgtattgt ttttttttgg ggggggggtg gccagaatag tgggtcatct aataaaactg 6075

ccatttaaaa gatcaaaaaa aaaaaaaaaa aaaaaaaaa 6114

<210> 24

<211> 698

<212> PRT

<213> 小鼠(Mus musculus)

<400> 24

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met

370 375 380

Asn Pro Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys Pro Gln Val

385 390 395 400

His Ser Glu Ser Arg Leu Ala Gln Ser Asn Gln Val Pro Val Gln Pro

405 410 415

Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr

420 425 430

Ala Ser Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro

435 440 445

Gln Lys Glu Pro Met Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr

450 455 460

Asp Gln Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln

465 470 475 480

Val Phe Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn

485 490 495

Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn

500 505 510

Ala Pro Val Pro Pro Ala Asn Glu Pro Glu Thr Leu Lys Gln Gln Ser

515 520 525

Gln Tyr Gln Ala Thr Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln

530 535 540

Val Glu Gln Thr Glu Leu Gln Gln Asp Gln Leu Gln Thr Val Val Gly

545 550 555 560

Thr Tyr His Gly Ser Gln Asp Gln Pro His Gln Val Pro Gly Asn His

565 570 575

Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Ser Gln Pro

580 585 590

Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg

595 600 605

Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly

610 615 620

Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr

625 630 635 640

Ser Gln Ser Gln Phe Thr Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg

645 650 655

Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro

660 665 670

Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly

675 680 685

Met Pro Gln Met Asn Thr Gln Gln Val Asn

690 695

<210> 25

<211> 3548

<212> DNA

<213> 小鼠(Mus musculus)

<220>

<221> CDS

<222> (179)..(2257)

<400> 25

gctggctggc taagtccctc ccgcgccggc tcttgtccca ctaggagcag ctcagagccg 60

cggggacagg gcgaagcggc ctgcgcccac ggagcgcacg tctctgttct caacgcagca 120

ccacccttgc ccccctcggc tgcccactcc agacgtccag cggctccgcg cgcgcacg 178

atg ccc tcg gcc acc agc cac agc gga agc ggc agc aaa tcg tcg gga 226

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

ccg ccg ccg ccg tcc ggt tcc tcc ggg agt gag gcg gcg gcc ggg gca 274

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

gct gcg ccg gct tct cag cat ccg gca acc ggc acc ggc gcc gtc cag 322

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

acc gag gcc atg aag cag att ctc ggc gta atc gac aag aaa ctt cgg 370

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

aac ctg gag aag aaa aag ggt aaa ctt gat gat tac cag gaa cga atg 418

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

aat aaa ggg gaa agg ctc aat caa gac cag ctg gat gcc gta tct aag 466

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

tac cag gaa gtc aca aat aat ttg gag ttt gca aag gaa tta cag agg 514

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

agt ttc atg gca tta agt caa gat att cag aaa aca ata aag aag aca 562

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

gca cgt cgg gaa cag ctt atg aga gaa gaa gca gaa cag aag cgc tta 610

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

aaa act gta ctt gag tta cag tat gta ttg gat aag ctg gga gat gat 658

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

gat gtg aga aca gat ctg aaa caa ggt ttg agt gga gtg cca ata ttg 706

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

tct gag gag gag ttg tca ttg ctg gat gag ttc tac aag ctc gta gat 754

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

cct gag cgt gac atg agt tta agg tta aat gag cag tat gaa cat gcc 802

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

tca att cac ttg tgg gat ttg ctg gaa ggg aaa gaa aag cct gtg tgt 850

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

gga aca acc tat aaa gct cta aag gaa att gtt gag cgt gtt ttc cag 898

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

tca aac tac ttt gat agc act cac aat cat caa aat ggg ttg tgt gag 946

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

gag gaa gag gcg gct tca gcg ccc aca gtg gag gac cag gta gct gaa 994

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

gct gaa cct gag cca gcg gaa gaa tac aca gag caa agt gag gtt gaa 1042

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

tca aca gag tat gtc aat agg cag ttc atg gca gaa aca cag ttc agc 1090

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

agt ggt gag aag gag caa gtg gat gag tgg aca gtt gaa aca gtt gag 1138

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

gtt gta aac tca ctc cag cag caa cct cag gct gcg tcc cct tca gtc 1186

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

cca gag ccc cac tct ttg act cca gtg gct cag tca gat cca ctt gtg 1234

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

aga agg cag cgt gta caa gat ctt atg gca caa atg caa ggg ccc tat 1282

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

aat ttc ata cag gat tca atg ttg gat ttt gaa aat cag acg ctt gat 1330

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

cct gcc att gta tcc gca cag cct atg aac cct acc cag aac atg gat 1378

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

atg cct cag ctg gtt tgc cct cag gtt cat tct gaa tct aga ctt gcc 1426

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

caa tct aat caa gtt cct gta caa cca gaa gcc aca cag gtt cct ttg 1474

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

gtt tca tcc aca agt gag ggg tat aca gca tct cag ccc ttg tac cag 1522

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

cca tct cat gct acg gag cag cgg ccg cag aaa gag cca atg gat cag 1570

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

att cag gca aca ata tct ttg aat aca gac cag act aca gca tcc tca 1618

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

tcc ctt cct gct gct tct cag cct caa gtg ttc cag gct ggg aca agt 1666

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

aaa cct ttg cac agc agt gga atc aat gta aat gca gct cca ttc cag 1714

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

tcc atg caa acg gtg ttc aat atg aat gct cca gtc cct cct gct aat 1762

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

gaa cca gaa acg tta aaa caa cag agt cag tac cag gcc act tat aac 1810

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

cag agt ttt tcc agt cag cct cac caa gtg gaa caa aca gag ctt caa 1858

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

caa gac caa ctg caa acg gtg gtt ggc act tac cat gga tcc cag gac 1906

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

cag cct cat caa gtg cct ggt aac cac cag caa ccc cca cag cag aac 1954

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

act ggc ttt cca cgt agc agt cag cct tat tac aac agt cgt ggg gta 2002

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

tct cga gga ggg tct cgt ggt gcc aga ggc ttg atg aat gga tac agg 2050

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

ggc cct gcc aat gga ttt aga gga gga tat gat ggt tac cgc cct tca 2098

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

ttc tcg aac act cca aac agt ggt tat tca cag tct cag ttc act gct 2146

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

ccc cgg gac tac tct ggt tac cag cgg gat gga tat cag cag aat ttc 2194

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

aag cga ggc tct ggg cag agt gga cca cgg gga gcc cca cga ggt aat 2242

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Asn

675 680 685

ata ttg tgg tgg tga tcctagctcc tatgtggagc ttctgttctg gccttggaag 2297

Ile Leu Trp Trp

690

aactgttcat agtccgcatg taggttacat gttaggaata catttatctt ttccagactt 2357

gttgctaaag attaaatgaa atgctctgtt tctaaaattt catcttgaat ccaaatttta 2417

atttttgaat gactttccct gctgttgtct tcaaaatcag aacattttct ctgcctcaga 2477

aaagcgtttt tccaactgga aatttatttt tcaggtctta aaacctgcta aatgttttta 2537

ggaagtacct actgaaactt tttgtaagac atttttggaa cgagcttgaa catttatata 2597

aatttattac cctctttgat ttttgaaaca tgcatattat atttaggctg agaagccctt 2657

caaatggcca gataagccac agttttagct agagaaccat ttagaattga cataactaat 2717

ctaaacttga acacttttag gaccaatgtt agtgttctaa ataccaacat atttctgatg 2777

tttaaacaga tctcccaaat tcttaggacc ttgatgtcat taaaatttag aatgacaagc 2837

ttaagaggct ttagtttcat ttgtttttca agtaatgaaa aataatttct tacatgggca 2897

gatagttaat ttgttgaaca attacaggta gcatttcatg taatctgatg ttctaaatgg 2957

ttctcttatt gaaggaggtt aaagaattag gtttcttaca gtttttggct ggccatgaca 3017

tgtataaaat gtatattaag gaggaattat aaagtacttt aatttgaatg ctagtggcaa 3077

ttgatcatta agaaagtact ttaaagcaaa aggttaatgg gtcatctggg aaaaatactg 3137

aagtatcaaa ggtatttgca tgtgaatgtg ggttatgttc ttctatccca ccttgtagca 3197

tattctatga aagttgagtt aaatgatagc taaaatatct gtttcaacag catgtaaaaa 3257

gttattttaa ctgttacaag tcattataca attttgaatg ttctgtagtt tctttttaac 3317

agtttaggta caaaggtctg ttttcattct ggtgcttttt attaattttg atagtatgat 3377

gtcacttcct attgaaatgt aagctagcgt gtaccttaga atgtgagctc catgagagca 3437

ggtaccttgt ttgtcttcac tgctgtatct attcccaacg cctcatgaca gtgcctggca 3497

catagtaggc actcaataaa tacttgttga atgaatgaaa aaaaaaaaaa a 3548

<210> 26

<211> 692

<212> PRT

<213> 小鼠(Mus musculus)

<400> 26

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Asn

675 680 685

Ile Leu Trp Trp

690

<210> 27

<211> 3508

<212> DNA

<213> 小鼠(Mus musculus)

<220>

<221> CDS

<222> (139)..(2217)

<400> 27

cccaccgcgc gcgcgcgtag ccgcctgccc gcccgcccgc tgcgcgtttt gtcccgcgtc 60

tctccccgtc cgtctcctga cttgctggtc ttgtccttcc ctcccgcttt tttcctctcc 120

tctcttctcg gtctaaag atg ccc tcg gcc acc agc cac agc gga agc ggc 171

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly

1 5 10

agc aaa tcg tcg gga ccg ccg ccg ccg tcc ggt tcc tcc ggg agt gag 219

Ser Lys Ser Ser Gly Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu

15 20 25

gcg gcg gcc ggg gca gct gcg ccg gct tct cag cat ccg gca acc ggc 267

Ala Ala Ala Gly Ala Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly

30 35 40

acc ggc gcc gtc cag acc gag gcc atg aag cag att ctc ggc gta atc 315

Thr Gly Ala Val Gln Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile

45 50 55

gac aag aaa ctt cgg aac ctg gag aag aaa aag ggt aaa ctt gat gat 363

Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp

60 65 70 75

tac cag gaa cga atg aat aaa ggg gaa agg ctc aat caa gac cag ctg 411

Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu

80 85 90

gat gcc gta tct aag tac cag gaa gtc aca aat aat ttg gag ttt gca 459

Asp Ala Val Ser Lys Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala

95 100 105

aag gaa tta cag agg agt ttc atg gca tta agt caa gat att cag aaa 507

Lys Glu Leu Gln Arg Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys

110 115 120

aca ata aag aag aca gca cgt cgg gaa cag ctt atg aga gaa gaa gca 555

Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala

125 130 135

gaa cag aag cgc tta aaa act gta ctt gag tta cag tat gta ttg gat 603

Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp

140 145 150 155

aag ctg gga gat gat gat gtg aga aca gat ctg aaa caa ggt ttg agt 651

Lys Leu Gly Asp Asp Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser

160 165 170

gga gtg cca ata ttg tct gag gag gag ttg tca ttg ctg gat gag ttc 699

Gly Val Pro Ile Leu Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe

175 180 185

tac aag ctc gta gat cct gag cgt gac atg agt tta agg tta aat gag 747

Tyr Lys Leu Val Asp Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu

190 195 200

cag tat gaa cat gcc tca att cac ttg tgg gat ttg ctg gaa ggg aaa 795

Gln Tyr Glu His Ala Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys

205 210 215

gaa aag cct gtg tgt gga aca acc tat aaa gct cta aag gaa att gtt 843

Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val

220 225 230 235

gag cgt gtt ttc cag tca aac tac ttt gat agc act cac aat cat caa 891

Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln

240 245 250

aat ggg ttg tgt gag gag gaa gag gcg gct tca gcg ccc aca gtg gag 939

Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu

255 260 265

gac cag gta gct gaa gct gaa cct gag cca gcg gaa gaa tac aca gag 987

Asp Gln Val Ala Glu Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu

270 275 280

caa agt gag gtt gaa tca aca gag tat gtc aat agg cag ttc atg gca 1035

Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala

285 290 295

gaa aca cag ttc agc agt ggt gag aag gag caa gtg gat gag tgg aca 1083

Glu Thr Gln Phe Ser Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr

300 305 310 315

gtt gaa aca gtt gag gtt gta aac tca ctc cag cag caa cct cag gct 1131

Val Glu Thr Val Glu Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala

320 325 330

gcg tcc cct tca gtc cca gag ccc cac tct ttg act cca gtg gct cag 1179

Ala Ser Pro Ser Val Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln

335 340 345

tca gat cca ctt gtg aga agg cag cgt gta caa gat ctt atg gca caa 1227

Ser Asp Pro Leu Val Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln

350 355 360

atg caa ggg ccc tat aat ttc ata cag gat tca atg ttg gat ttt gaa 1275

Met Gln Gly Pro Tyr Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu

365 370 375

aat cag acg ctt gat cct gcc att gta tcc gca cag cct atg aac cct 1323

Asn Gln Thr Leu Asp Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro

380 385 390 395

acc cag aac atg gat atg cct cag ctg gtt tgc cct cag gtt cat tct 1371

Thr Gln Asn Met Asp Met Pro Gln Leu Val Cys Pro Gln Val His Ser

400 405 410

gaa tct aga ctt gcc caa tct aat caa gtt cct gta caa cca gaa gcc 1419

Glu Ser Arg Leu Ala Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala

415 420 425

aca cag gtt cct ttg gtt tca tcc aca agt gag ggg tat aca gca tct 1467

Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser

430 435 440

cag ccc ttg tac cag cca tct cat gct acg gag cag cgg ccg cag aaa 1515

Gln Pro Leu Tyr Gln Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys

445 450 455

gag cca atg gat cag att cag gca aca ata tct ttg aat aca gac cag 1563

Glu Pro Met Asp Gln Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln

460 465 470 475

act aca gca tcc tca tcc ctt cct gct gct tct cag cct caa gtg ttc 1611

Thr Thr Ala Ser Ser Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe

480 485 490

cag gct ggg aca agt aaa cct ttg cac agc agt gga atc aat gta aat 1659

Gln Ala Gly Thr Ser Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn

495 500 505

gca gct cca ttc cag tcc atg caa acg gtg ttc aat atg aat gct cca 1707

Ala Ala Pro Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro

510 515 520

gtc cct cct gct aat gaa cca gaa acg tta aaa caa cag agt cag tac 1755

Val Pro Pro Ala Asn Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr

525 530 535

cag gcc act tat aac cag agt ttt tcc agt cag cct cac caa gtg gaa 1803

Gln Ala Thr Tyr Asn Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu

540 545 550 555

caa aca gag ctt caa caa gac caa ctg caa acg gtg gtt ggc act tac 1851

Gln Thr Glu Leu Gln Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr

560 565 570

cat gga tcc cag gac cag cct cat caa gtg cct ggt aac cac cag caa 1899

His Gly Ser Gln Asp Gln Pro His Gln Val Pro Gly Asn His Gln Gln

575 580 585

ccc cca cag cag aac act ggc ttt cca cgt agc agt cag cct tat tac 1947

Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr

590 595 600

aac agt cgt ggg gta tct cga gga ggg tct cgt ggt gcc aga ggc ttg 1995

Asn Ser Arg Gly Val Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu

605 610 615

atg aat gga tac agg ggc cct gcc aat gga ttt aga gga gga tat gat 2043

Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp

620 625 630 635

ggt tac cgc cct tca ttc tcg aac act cca aac agt ggt tat tca cag 2091

Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln

640 645 650

tct cag ttc act gct ccc cgg gac tac tct ggt tac cag cgg gat gga 2139

Ser Gln Phe Thr Ala Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly

655 660 665

tat cag cag aat ttc aag cga ggc tct ggg cag agt gga cca cgg gga 2187

Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly

670 675 680

gcc cca cga ggt aat ata ttg tgg tgg tga tcctagctcc tatgtggagc 2237

Ala Pro Arg Gly Asn Ile Leu Trp Trp

685 690

ttctgttctg gccttggaag aactgttcat agtccgcatg taggttacat gttaggaata 2297

catttatctt ttccagactt gttgctaaag attaaatgaa atgctctgtt tctaaaattt 2357

catcttgaat ccaaatttta atttttgaat gactttccct gctgttgtct tcaaaatcag 2417

aacattttct ctgcctcaga aaagcgtttt tccaactgga aatttatttt tcaggtctta 2477

aaacctgcta aatgttttta ggaagtacct actgaaactt tttgtaagac atttttggaa 2537

cgagcttgaa catttatata aatttattac cctctttgat ttttgaaaca tgcatattat 2597

atttaggctg agaagccctt caaatggcca gataagccac agttttagct agagaaccat 2657

ttagaattga cataactaat ctaaacttga acacttttag gaccaatgtt agtgttctaa 2717

ataccaacat atttctgatg tttaaacaga tctcccaaat tcttaggacc ttgatgtcat 2777

taaaatttag aatgacaagc ttaagaggct ttagtttcat ttgtttttca agtaatgaaa 2837

aataatttct tacatgggca gatagttaat ttgttgaaca attacaggta gcatttcatg 2897

taatctgatg ttctaaatgg ttctcttatt gaaggaggtt aaagaattag gtttcttaca 2957

gtttttggct ggccatgaca tgtataaaat gtatattaag gaggaattat aaagtacttt 3017

aatttgaatg ctagtggcaa ttgatcatta agaaagtact ttaaagcaaa aggttaatgg 3077

gtcatctggg aaaaatactg aagtatcaaa ggtatttgca tgtgaatgtg ggttatgttc 3137

ttctatccca ccttgtagca tattctatga aagttgagtt aaatgatagc taaaatatct 3197

gtttcaacag catgtaaaaa gttattttaa ctgttacaag tcattataca attttgaatg 3257

ttctgtagtt tctttttaac agtttaggta caaaggtctg ttttcattct ggtgcttttt 3317

attaattttg atagtatgat gtcacttcct attgaaatgt aagctagcgt gtaccttaga 3377

atgtgagctc catgagagca ggtaccttgt ttgtcttcac tgctgtatct attcccaacg 3437

cctcatgaca gtgcctggca catagtaggc actcaataaa tacttgttga atgaatgaaa 3497

aaaaaaaaaa a 3508

<210> 28

<211> 692

<212> PRT

<213> 小鼠(Mus musculus)

<400> 28

Met Pro Ser Ala Thr Ser His Ser Gly Ser Gly Ser Lys Ser Ser Gly

1 5 10 15

Pro Pro Pro Pro Ser Gly Ser Ser Gly Ser Glu Ala Ala Ala Gly Ala

20 25 30

Ala Ala Pro Ala Ser Gln His Pro Ala Thr Gly Thr Gly Ala Val Gln

35 40 45

Thr Glu Ala Met Lys Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg

50 55 60

Asn Leu Glu Lys Lys Lys Gly Lys Leu Asp Asp Tyr Gln Glu Arg Met

65 70 75 80

Asn Lys Gly Glu Arg Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys

85 90 95

Tyr Gln Glu Val Thr Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg

100 105 110

Ser Phe Met Ala Leu Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr

115 120 125

Ala Arg Arg Glu Gln Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu

130 135 140

Lys Thr Val Leu Glu Leu Gln Tyr Val Leu Asp Lys Leu Gly Asp Asp

145 150 155 160

Asp Val Arg Thr Asp Leu Lys Gln Gly Leu Ser Gly Val Pro Ile Leu

165 170 175

Ser Glu Glu Glu Leu Ser Leu Leu Asp Glu Phe Tyr Lys Leu Val Asp

180 185 190

Pro Glu Arg Asp Met Ser Leu Arg Leu Asn Glu Gln Tyr Glu His Ala

195 200 205

Ser Ile His Leu Trp Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys

210 215 220

Gly Thr Thr Tyr Lys Ala Leu Lys Glu Ile Val Glu Arg Val Phe Gln

225 230 235 240

Ser Asn Tyr Phe Asp Ser Thr His Asn His Gln Asn Gly Leu Cys Glu

245 250 255

Glu Glu Glu Ala Ala Ser Ala Pro Thr Val Glu Asp Gln Val Ala Glu

260 265 270

Ala Glu Pro Glu Pro Ala Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu

275 280 285

Ser Thr Glu Tyr Val Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser

290 295 300

Ser Gly Glu Lys Glu Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu

305 310 315 320

Val Val Asn Ser Leu Gln Gln Gln Pro Gln Ala Ala Ser Pro Ser Val

325 330 335

Pro Glu Pro His Ser Leu Thr Pro Val Ala Gln Ser Asp Pro Leu Val

340 345 350

Arg Arg Gln Arg Val Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr

355 360 365

Asn Phe Ile Gln Asp Ser Met Leu Asp Phe Glu Asn Gln Thr Leu Asp

370 375 380

Pro Ala Ile Val Ser Ala Gln Pro Met Asn Pro Thr Gln Asn Met Asp

385 390 395 400

Met Pro Gln Leu Val Cys Pro Gln Val His Ser Glu Ser Arg Leu Ala

405 410 415

Gln Ser Asn Gln Val Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu

420 425 430

Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala Ser Gln Pro Leu Tyr Gln

435 440 445

Pro Ser His Ala Thr Glu Gln Arg Pro Gln Lys Glu Pro Met Asp Gln

450 455 460

Ile Gln Ala Thr Ile Ser Leu Asn Thr Asp Gln Thr Thr Ala Ser Ser

465 470 475 480

Ser Leu Pro Ala Ala Ser Gln Pro Gln Val Phe Gln Ala Gly Thr Ser

485 490 495

Lys Pro Leu His Ser Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln

500 505 510

Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro Ala Asn

515 520 525

Glu Pro Glu Thr Leu Lys Gln Gln Ser Gln Tyr Gln Ala Thr Tyr Asn

530 535 540

Gln Ser Phe Ser Ser Gln Pro His Gln Val Glu Gln Thr Glu Leu Gln

545 550 555 560

Gln Asp Gln Leu Gln Thr Val Val Gly Thr Tyr His Gly Ser Gln Asp

565 570 575

Gln Pro His Gln Val Pro Gly Asn His Gln Gln Pro Pro Gln Gln Asn

580 585 590

Thr Gly Phe Pro Arg Ser Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val

595 600 605

Ser Arg Gly Gly Ser Arg Gly Ala Arg Gly Leu Met Asn Gly Tyr Arg

610 615 620

Gly Pro Ala Asn Gly Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser

625 630 635 640

Phe Ser Asn Thr Pro Asn Ser Gly Tyr Ser Gln Ser Gln Phe Thr Ala

645 650 655

Pro Arg Asp Tyr Ser Gly Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe

660 665 670

Lys Arg Gly Ser Gly Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Asn

675 680 685

Ile Leu Trp Trp

690

<210> 29

<211> 2109

<212> DNA

<213> 鸡(Gallus gallus)

<220>

<221> CDS

<222> (1)..(2109)

<400> 29

atg ccc tcg gct acc aac ggc acc atg gcg agc agc agc ggg aag gcg 48

Met Pro Ser Ala Thr Asn Gly Thr Met Ala Ser Ser Ser Gly Lys Ala

1 5 10 15

ggc ccg ggc ggc aac gag cag gcc ccg gcg gcg gca gcg gcg gcc ccg 96

Gly Pro Gly Gly Asn Glu Gln Ala Pro Ala Ala Ala Ala Ala Ala Pro

20 25 30

cag gcg tcg ggc ggc agc atc acc tcg gtt cag acc gag gcc atg aag 144

Gln Ala Ser Gly Gly Ser Ile Thr Ser Val Gln Thr Glu Ala Met Lys

35 40 45

cag atc ttg gga gtg atc gac aaa aag ctc cgc aac ctc gag aag aaa 192

Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys

50 55 60

aag agc aaa ctt gac gat tac cag gaa cga atg aac aag ggg gaa cgt 240

Lys Ser Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg

65 70 75 80

cta aat caa gat caa ctg gat gca gtg tca aaa tac cag gaa gtg aca 288

Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr

85 90 95

aat aac ctg gaa ttc gct aaa gaa ctg cag agg agc ttt atg gca ctg 336

Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu

100 105 110

agc caa gat atc cag aaa aca ata aaa aag acg gct cgc agg gag cag 384

Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln

115 120 125

ctg atg aga gaa gag gct gag cag aag cgt tta aag act gtg cta gag 432

Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu

130 135 140

ctg cag ttc att ttg gac aag ttg ggt gac gat gaa gtg cgc agt gac 480

Leu Gln Phe Ile Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Ser Asp

145 150 155 160

ttg aaa caa gga tca aat gga gta ccg gta ctg aca gag gag gaa ctg 528

Leu Lys Gln Gly Ser Asn Gly Val Pro Val Leu Thr Glu Glu Glu Leu

165 170 175

aca atg ctg gat gaa ttt tac aag cta gtt tac cct gaa agg gac atg 576

Thr Met Leu Asp Glu Phe Tyr Lys Leu Val Tyr Pro Glu Arg Asp Met

180 185 190

aac atg agg ttg aat gag cag tat gag caa gca tct gtt cac ctg tgg 624

Asn Met Arg Leu Asn Glu Gln Tyr Glu Gln Ala Ser Val His Leu Trp

195 200 205

gac tta ctg gaa ggg aag gaa aaa ccc gtt tgt gga aca acc tat aaa 672

Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys

210 215 220

gcc ctg aag gag gtt gtt gaa cgt att ctt caa act agt tac ttt gat 720

Ala Leu Lys Glu Val Val Glu Arg Ile Leu Gln Thr Ser Tyr Phe Asp

225 230 235 240

agc acc cat aac cat cag aac ggg tta tgt gag gaa gaa gag gca gca 768

Ser Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala

245 250 255

ccc aca cct gca gta gaa gac act gta gca gaa gct gag cct gat cca 816

Pro Thr Pro Ala Val Glu Asp Thr Val Ala Glu Ala Glu Pro Asp Pro

260 265 270

gca gaa gaa ttt act gaa cct act gaa gtt gaa tcg act gag tat gta 864

Ala Glu Glu Phe Thr Glu Pro Thr Glu Val Glu Ser Thr Glu Tyr Val

275 280 285

aac aga caa ttc atg gca gag act cag ttc agc agt agt gag aag gaa 912

Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Ser Glu Lys Glu

290 295 300

cag gta gat gag tgg aca gtt gaa acg gtt gag gtt gta aat tca ctg 960

Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu

305 310 315 320

cag caa caa aca caa gct aca tct cct cca gtt cct gaa cct cat aca 1008

Gln Gln Gln Thr Gln Ala Thr Ser Pro Pro Val Pro Glu Pro His Thr

325 330 335

ctc act act gtg gct caa gca gat cct ctt gtt aga aga cag aga gta 1056

Leu Thr Thr Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val

340 345 350

cag gac ctt atg gcc cag atg cag ggt cca tat aac ttc atg cag gac 1104

Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Met Gln Asp

355 360 365

tct atg ctg gag ttt gag aac cag aca ctt gat cct gcc att gta tct 1152

Ser Met Leu Glu Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser

370 375 380

gca cag ccc atg aat cca gca cag aat ttg gac atg ccg caa atg gtc 1200

Ala Gln Pro Met Asn Pro Ala Gln Asn Leu Asp Met Pro Gln Met Val

385 390 395 400

tgc cct cca gtt cat act gag tca aga ctt gcc cag cct aat caa gtt 1248

Cys Pro Pro Val His Thr Glu Ser Arg Leu Ala Gln Pro Asn Gln Val

405 410 415

cct gtg caa cca gaa gct acg cag gtt ccc ttg gtt tca tct aca agt 1296

Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser

420 425 430

gag gga tat aca gcc tcc cag ccc atg tat cag cct tct cat acc aca 1344

Glu Gly Tyr Thr Ala Ser Gln Pro Met Tyr Gln Pro Ser His Thr Thr

435 440 445

gag caa cgg cca cag aag gaa tcc att gac cag att cag gct tca atg 1392

Glu Gln Arg Pro Gln Lys Glu Ser Ile Asp Gln Ile Gln Ala Ser Met

450 455 460

tca ctg aat gca gac cag acc ccg tca tca tca tca ctt ccc act gca 1440

Ser Leu Asn Ala Asp Gln Thr Pro Ser Ser Ser Ser Leu Pro Thr Ala

465 470 475 480

tcc cag ccg caa gtt ttc caa gct gga tct agc aaa cct ttg cat agc 1488

Ser Gln Pro Gln Val Phe Gln Ala Gly Ser Ser Lys Pro Leu His Ser

485 490 495

agc gga atc aat gtt aat gca gct cca ttc caa tcc atg caa aca gta 1536

Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val

500 505 510

ttc aac atg aat gca cct gtt cct cct gtt aat gag cca gaa gcc ctt 1584

Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Ala Leu

515 520 525

aag caa caa aat cag tac cag gcc agt tac aac cag agt ttc tcc aat 1632

Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Asn

530 535 540

cag cca cac caa gta gaa caa tca gat ctt cag caa gaa cag ctc cag 1680

Gln Pro His Gln Val Glu Gln Ser Asp Leu Gln Gln Glu Gln Leu Gln

545 550 555 560

aca gtg gtt ggt act tac cat ggt tct ccg gac cag acc cat caa gtg 1728

Thr Val Val Gly Thr Tyr His Gly Ser Pro Asp Gln Thr His Gln Val

565 570 575

gca gga aac cac cag caa cct ccc cag cag aat act gga ttt cca cgc 1776

Ala Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg

580 585 590

aac agt cag cct tat tac aac agt cgg gga gtg tct cgt ggt gga tca 1824

Asn Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser

595 600 605

cgt ggg act cgt gga ttg atg aat ggt tac agg gga cct gca aat gga 1872

Arg Gly Thr Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly

610 615 620

ttt aga gga gga tat gat ggc tac cgt cct tca ttt tcc aac act ccg 1920

Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro

625 630 635 640

aac agt ggt tac acg cag ccc caa ttt aat gct cct cga gat tat tca 1968

Asn Ser Gly Tyr Thr Gln Pro Gln Phe Asn Ala Pro Arg Asp Tyr Ser

645 650 655

aac tac cag cgg gat gga tat cag cag aac ttc aaa cgt ggt tct gga 2016

Asn Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly

660 665 670

caa agt ggg cct cgg gga gct cct cga ggt cgt gga ggg ccc cca aga 2064

Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg

675 680 685

cca aac aga ggg atg cct caa atg aac gct cag caa gtg aat taa 2109

Pro Asn Arg Gly Met Pro Gln Met Asn Ala Gln Gln Val Asn

690 695 700

<210> 30

<211> 702

<212> PRT

<213> 鸡(Gallus gallus)

<400> 30

Met Pro Ser Ala Thr Asn Gly Thr Met Ala Ser Ser Ser Gly Lys Ala

1 5 10 15

Gly Pro Gly Gly Asn Glu Gln Ala Pro Ala Ala Ala Ala Ala Ala Pro

20 25 30

Gln Ala Ser Gly Gly Ser Ile Thr Ser Val Gln Thr Glu Ala Met Lys

35 40 45

Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys

50 55 60

Lys Ser Lys Leu Asp Asp Tyr Gln Glu Arg Met Asn Lys Gly Glu Arg

65 70 75 80

Leu Asn Gln Asp Gln Leu Asp Ala Val Ser Lys Tyr Gln Glu Val Thr

85 90 95

Asn Asn Leu Glu Phe Ala Lys Glu Leu Gln Arg Ser Phe Met Ala Leu

100 105 110

Ser Gln Asp Ile Gln Lys Thr Ile Lys Lys Thr Ala Arg Arg Glu Gln

115 120 125

Leu Met Arg Glu Glu Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu

130 135 140

Leu Gln Phe Ile Leu Asp Lys Leu Gly Asp Asp Glu Val Arg Ser Asp

145 150 155 160

Leu Lys Gln Gly Ser Asn Gly Val Pro Val Leu Thr Glu Glu Glu Leu

165 170 175

Thr Met Leu Asp Glu Phe Tyr Lys Leu Val Tyr Pro Glu Arg Asp Met

180 185 190

Asn Met Arg Leu Asn Glu Gln Tyr Glu Gln Ala Ser Val His Leu Trp

195 200 205

Asp Leu Leu Glu Gly Lys Glu Lys Pro Val Cys Gly Thr Thr Tyr Lys

210 215 220

Ala Leu Lys Glu Val Val Glu Arg Ile Leu Gln Thr Ser Tyr Phe Asp

225 230 235 240

Ser Thr His Asn His Gln Asn Gly Leu Cys Glu Glu Glu Glu Ala Ala

245 250 255

Pro Thr Pro Ala Val Glu Asp Thr Val Ala Glu Ala Glu Pro Asp Pro

260 265 270

Ala Glu Glu Phe Thr Glu Pro Thr Glu Val Glu Ser Thr Glu Tyr Val

275 280 285

Asn Arg Gln Phe Met Ala Glu Thr Gln Phe Ser Ser Ser Glu Lys Glu

290 295 300

Gln Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu

305 310 315 320

Gln Gln Gln Thr Gln Ala Thr Ser Pro Pro Val Pro Glu Pro His Thr

325 330 335

Leu Thr Thr Val Ala Gln Ala Asp Pro Leu Val Arg Arg Gln Arg Val

340 345 350

Gln Asp Leu Met Ala Gln Met Gln Gly Pro Tyr Asn Phe Met Gln Asp

355 360 365

Ser Met Leu Glu Phe Glu Asn Gln Thr Leu Asp Pro Ala Ile Val Ser

370 375 380

Ala Gln Pro Met Asn Pro Ala Gln Asn Leu Asp Met Pro Gln Met Val

385 390 395 400

Cys Pro Pro Val His Thr Glu Ser Arg Leu Ala Gln Pro Asn Gln Val

405 410 415

Pro Val Gln Pro Glu Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser

420 425 430

Glu Gly Tyr Thr Ala Ser Gln Pro Met Tyr Gln Pro Ser His Thr Thr

435 440 445

Glu Gln Arg Pro Gln Lys Glu Ser Ile Asp Gln Ile Gln Ala Ser Met

450 455 460

Ser Leu Asn Ala Asp Gln Thr Pro Ser Ser Ser Ser Leu Pro Thr Ala

465 470 475 480

Ser Gln Pro Gln Val Phe Gln Ala Gly Ser Ser Lys Pro Leu His Ser

485 490 495

Ser Gly Ile Asn Val Asn Ala Ala Pro Phe Gln Ser Met Gln Thr Val

500 505 510

Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Ala Leu

515 520 525

Lys Gln Gln Asn Gln Tyr Gln Ala Ser Tyr Asn Gln Ser Phe Ser Asn

530 535 540

Gln Pro His Gln Val Glu Gln Ser Asp Leu Gln Gln Glu Gln Leu Gln

545 550 555 560

Thr Val Val Gly Thr Tyr His Gly Ser Pro Asp Gln Thr His Gln Val

565 570 575

Ala Gly Asn His Gln Gln Pro Pro Gln Gln Asn Thr Gly Phe Pro Arg

580 585 590

Asn Ser Gln Pro Tyr Tyr Asn Ser Arg Gly Val Ser Arg Gly Gly Ser

595 600 605

Arg Gly Thr Arg Gly Leu Met Asn Gly Tyr Arg Gly Pro Ala Asn Gly

610 615 620

Phe Arg Gly Gly Tyr Asp Gly Tyr Arg Pro Ser Phe Ser Asn Thr Pro

625 630 635 640

Asn Ser Gly Tyr Thr Gln Pro Gln Phe Asn Ala Pro Arg Asp Tyr Ser

645 650 655

Asn Tyr Gln Arg Asp Gly Tyr Gln Gln Asn Phe Lys Arg Gly Ser Gly

660 665 670

Gln Ser Gly Pro Arg Gly Ala Pro Arg Gly Arg Gly Gly Pro Pro Arg

675 680 685

Pro Asn Arg Gly Met Pro Gln Met Asn Ala Gln Gln Val Asn

690 695 700

<210> 31

<211> 63

<212> PRT

<213> 人(Homo sapiens)

<400> 31

Glu Glu Tyr Thr Glu Gln Ser Glu Val Glu Ser Thr Glu Tyr Val Asn

1 5 10 15

Arg Gln Phe Met Ala Glu Thr Gln Phe Thr Ser Gly Glu Lys Glu Gln

20 25 30

Val Asp Glu Trp Thr Val Glu Thr Val Glu Val Val Asn Ser Leu Gln

35 40 45

Gln Gln Pro Gln Ala Ala Ser Pro Ser Val Pro Glu Pro His Ser

50 55 60

<210> 32

<211> 18

<212> PRT

<213> 人(Homo sapiens)

<400> 32

Val Phe Asn Met Asn Ala Pro Val Pro Pro Val Asn Glu Pro Glu Thr

1 5 10 15

Leu Lys

<210> 33

<211> 16

<212> PRT

<213> 人(Homo sapiens)

<400> 33

Ala Thr Gln Val Pro Leu Val Ser Ser Thr Ser Glu Gly Tyr Thr Ala

1 5 10 15

<210> 34

<211> 25

<212> PRT

<213> 人(Homo sapiens)

<400> 34

Gln Ile Leu Gly Val Ile Asp Lys Lys Leu Arg Asn Leu Glu Lys Lys

1 5 10 15

Lys Gly Lys Leu Asp Asp Tyr Gln Glu

20 25

<210> 35

<211> 23

<212> PRT

<213> 人(Homo sapiens)

<400> 35

Pro Arg Gly Arg Gly Gly Pro Pro Arg Pro Asn Arg Gly Met Pro Gln

1 5 10 15

Met Asn Thr Gln Gln Val Asn

20

<210> 36

<211> 5

<212> PRT

<213> 鸡(Gallus gallus)

<400> 36

Ser Tyr Gln Met Asn

1 5

<210> 37

<211> 17

<212> PRT

<213> 鸡(Gallus gallus)

<400> 37

Ala Ile Asn Lys Phe Gly Asn Ser Thr Gly His Gly Ala Ala Val Lys

1 5 10 15

Gly

<210> 38

<211> 19

<212> PRT

<213> 鸡(Gallus gallus)

<400> 38

His Ala Tyr Gly Tyr Cys Gly Ser Gly Thr Trp Cys Ala Ala Gly Glu

1 5 10 15

Ile Asp Ala

<210> 39

<211> 128

<212> PRT

<213> 鸡(Gallus gallus)

<400> 39

Ala Val Thr Leu Asp Glu Ser Gly Gly Gly Leu Gln Met Ser Arg Gly

1 5 10 15

Gly Leu Ser Leu Val Cys Lys Ala Ser Gly Phe Asp Phe Ser Ser Tyr

20 25 30

Gln Met Asn Trp Ile Arg Gln Ala Pro Gly Lys Gly Leu Glu Phe Val

35 40 45

Ala Ala Ile Asn Lys Phe Gly Asn Ser Thr Gly His Gly Ala Ala Val

50 55 60

Lys Gly Arg Val Thr Ile Ser Arg Asp Asn Gly Gln Ser Thr Val Arg

65 70 75 80

Leu Gln Leu Asn Asn Leu Arg Ala Glu Asp Thr Ala Ile Tyr Phe Cys

85 90 95

Thr Lys His Ala Tyr Gly Tyr Cys Gly Ser Gly Thr Trp Cys Ala Ala

100 105 110

Gly Glu Ile Asp Ala Trp Gly His Gly Thr Glu Val Ile Val Ser Ser

115 120 125

<210> 40

<211> 9

<212> PRT

<213> 鸡(Gallus gallus)

<400> 40

Ser Gly Gly Gly Ser Tyr Ser Tyr Gly

1 5

<210> 41

<211> 7

<212> PRT

<213> 鸡(Gallus gallus)

<400> 41

Asn Asn Lys Arg Pro Ser Asp

1 5

<210> 42

<211> 10

<212> PRT

<213> 鸡(Gallus gallus)

<400> 42

Ser Gly Asp Ser Thr Asp Thr Ala Val Phe

1 5 10

<210> 43

<211> 108

<212> PRT

<213> 鸡(Gallus gallus)

<400> 43

Gln Ala Ala Ser Thr Gln Pro Ser Ser Val Ser Ala Asn Pro Gly Glu

1 5 10 15

Thr Val Glu Ile Thr Cys Ser Gly Gly Gly Ser Tyr Ser Tyr Gly Trp

20 25 30

Phe Gln Gln Lys Ser Pro Gly Ser Ala Pro Val Thr Val Ile Tyr Tyr

35 40 45

Asn Asn Lys Arg Pro Ser Asp Ile Pro Ser Arg Phe Ser Gly Ser Lys

50 55 60

Ser Gly Ser Thr Gly Thr Leu Thr Ile Thr Gly Val Gln Ala Asp Asp

65 70 75 80

Glu Ala Val Tyr Tyr Cys Gly Ser Gly Asp Ser Thr Asp Thr Ala Val

85 90 95

Phe Gly Ala Gly Thr Thr Leu Thr Val Leu Gly Gln

100 105

<210> 44

<211> 5

<212> PRT

<213> 兔(Rabbit)

<400> 44

Ser His Ser Leu Gly

1 5

<210> 45

<211> 16

<212> PRT

<213> 兔(Rabbit)

<400> 45

Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys Gly

1 5 10 15

<210> 46

<211> 13

<212> PRT

<213> 兔(Rabbit)

<400> 46

Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu

1 5 10

<210> 47

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 47

Glu Gln Ser Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 48

<211> 13

<212> PRT

<213> 兔(Rabbit)

<400> 48

Gln Ala Ser Gln Ser Leu Tyr Asn Asn Glu Asn Leu Ala

1 5 10

<210> 49

<211> 7

<212> PRT

<213> 兔(Rabbit)

<400> 49

Gly Ala Ser Thr Leu Ala Ser

1 5

<210> 50

<211> 13

<212> PRT

<213> 兔(Rabbit)

<400> 50

Leu Gly Glu Phe Ser Cys Gly Ser Ala Asp Cys Phe Ala

1 5 10

<210> 51

<211> 112

<212> PRT

<213> 人(Homo sapiens)

<400> 51

Gln Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp

1 5 10 15

Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn Glu

20 25 30

Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg Leu

35 40 45

Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe Ser

50 55 60

Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln

65 70 75 80

Cys Glu Asp Phe Ala Ile Tyr Tyr Cys Leu Gly Glu Phe Ser Cys Gly

85 90 95

Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys

100 105 110

<210> 52

<211> 4

<212> PRT

<213> 鸡(Gallus gallus)

<400> 52

Phe Asp Met Gly

1

<210> 53

<211> 17

<212> PRT

<213> 鸡(Gallus gallus)

<400> 53

Gln Ile Asn Asp Ala Gly Ser Arg Thr Trp Tyr Ala Thr Ala Val Lys

1 5 10 15

Gly

<210> 54

<211> 12

<212> PRT

<213> 鸡(Gallus gallus)

<400> 54

Gly Ser Gly Tyr Val Gly Ala Gly Ala Ile Asp Ala

1 5 10

<210> 55

<211> 120

<212> PRT

<213> 鸡(Gallus gallus)

<400> 55

Ala Val Thr Leu Asp Glu Ser Gly Gly Gly Leu Gln Thr Pro Gly Gly

1 5 10 15

Gly Leu Ser Leu Val Cys Lys Ala Ser Gly Phe Thr Phe Ser Ser Phe

20 25 30

Asp Met Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Phe Val

35 40 45

Ala Gln Ile Asn Asp Ala Gly Ser Arg Thr Trp Tyr Ala Thr Ala Val

50 55 60

Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Gly Gln Thr Thr Val Arg

65 70 75 80

Leu Gln Leu Asn Asn Leu Arg Ala Glu Asp Thr Gly Thr Tyr Tyr Cys

85 90 95

Thr Arg Gly Ser Gly Tyr Val Gly Ala Gly Ala Ile Asp Ala Trp Gly

100 105 110

His Gly Thr Glu Val Ile Val Ser

115 120

<210> 56

<211> 8

<212> PRT

<213> 鸡(Gallus gallus)

<400> 56

Ser Gly Gly Ser Gly Tyr Tyr Gly

1 5

<210> 57

<211> 7

<212> PRT

<213> 鸡(Gallus gallus)

<400> 57

Asn Asp Lys Arg Pro Ser Asp

1 5

<210> 58

<211> 10

<212> PRT

<213> 鸡(Gallus gallus)

<400> 58

Arg Tyr Asp Ser Thr Asp Ser Gly Ile Phe

1 5 10

<210> 59

<211> 105

<212> PRT

<213> 鸡(Gallus gallus)

<400> 59

Ala Ala Leu Thr Gln Pro Ser Ser Val Ser Ala Asn Pro Gly Glu Thr

1 5 10 15

Val Lys Ile Thr Cys Ser Gly Gly Ser Gly Tyr Tyr Gly Trp Tyr Gln

20 25 30

Gln Gln Lys Ser Pro Gly Ser Ala Pro Val Thr Val Ile Tyr Gln Asn

35 40 45

Asp Lys Arg Pro Ser Asp Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser

50 55 60

Gly Ser Thr Asn Thr Leu Thr Ile Thr Gly Val Gln Ala Glu Asp Glu

65 70 75 80

Ala Val Tyr Phe Cys Gly Arg Tyr Asp Ser Thr Asp Ser Gly Ile Phe

85 90 95

Gly Ala Gly Thr Thr Leu Thr Val Leu

100 105

<210> 60

<211> 6

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 60

Gly Ser Tyr Tyr Met Ser

1 5

<210> 61

<211> 17

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 61

Tyr Ile Tyr Ile Gly Asp Gly Val Thr Ala Tyr Ala Asn Trp Ala Lys

1 5 10 15

Gly

<210> 62

<211> 4

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 62

Gly Asn Lys Leu

1

<210> 63

<211> 112

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 63

Gln Ser Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Ala Ser

1 5 10 15

Leu Thr Leu Thr Cys Thr Ala Ser Gly Phe Ser Phe Ser Gly Ser Tyr

20 25 30

Tyr Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Ala Tyr Ile Tyr Ile Gly Asp Gly Val Thr Ala Tyr Ala Asn Trp Ala

50 55 60

Lys Gly Arg Phe Thr Ile Ser Lys Ala Ser Ser Thr Thr Val Thr Leu

65 70 75 80

Gln Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys Ala

85 90 95

Arg Gly Asn Lys Leu Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser

100 105 110

<210> 64

<211> 11

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 64

Gln Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala

1 5 10

<210> 65

<211> 7

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 65

Asp Ala Ser Asn Leu Asp Ser

1 5

<210> 66

<211> 14

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 66

Gln Cys Thr Ala Val Ser Ser Ala Thr Ile Tyr Gly Asn Ala

1 5 10

<210> 67

<211> 112

<212> PRT

<213> 兔(Oryctolagus cuniculus)

<400> 67

Asp Val Val Met Thr Gln Thr Pro Ala Ser Val Glu Ala Ala Val Gly

1 5 10 15

Gly Thr Val Thr Ile Lys Cys Gln Ala Ser Gln Ser Ile Ser Ser Tyr

20 25 30

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile

35 40 45

Tyr Asp Ala Ser Asn Leu Asp Ser Gly Val Pro Ser Arg Phe Lys Gly

50 55 60

Ser Gly Ser Gly Thr Asp Phe Thr Ile Thr Ile Ser Asp Leu Glu Cys

65 70 75 80

Ala Asp Ala Ala Thr Tyr Tyr Cys Gln Cys Thr Ala Val Ser Ser Ala

85 90 95

Thr Ile Tyr Gly Asn Ala Phe Gly Gly Gly Thr Glu Val Val Val Lys

100 105 110

<210> 68

<211> 127

<212> PRT

<213> 鸡(Gallus gallus)

<400> 68

Ala Val Thr Leu Asp Glu Ser Gly Gly Gly Leu Gln Thr Pro Gly Gly

1 5 10 15

Ala Leu Ser Leu Val Cys Lys Ala Ser Gly Phe Thr Phe Ser Gly Tyr

20 25 30

Asp Met Leu Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val

35 40 45

Ala Gly Ile Gly Ser Thr Gly Gly Gly Thr Asp Tyr Gly Ala Ala Val

50 55 60

Lys Gly Arg Ala Thr Ile Ser Arg Asp Asn Gly Gln Ser Thr Val Arg

65 70 75 80

Leu Gln Leu Asn Asn Leu Arg Ala Glu Asp Thr Ala Thr Tyr Tyr Cys

85 90 95

Ala Lys Val Ala Gly Gly Cys Asn Ser Gly Tyr Cys Arg Asp Ser Pro

100 105 110

Gly Ser Ile Asp Ala Trp Gly His Gly Thr Glu Val Ile Val Ser

115 120 125

<210> 69

<211> 107

<212> PRT

<213> 鸡(Gallus gallus)

<400> 69

Ala Val Thr Gln Gln Pro Ala Ser Val Ser Ala Asn Pro Gly Glu Thr

1 5 10 15

Val Lys Ile Thr Cys Ser Gly Gly Gly Ser Arg Asn Tyr Tyr Gly Trp

20 25 30

Tyr Gln Gln Lys Ser Pro Gly Ser Val Pro Val Thr Val Ile Tyr Tyr

35 40 45

Asp Asp Gln Arg Pro Ser Asn Ile Pro Ser Arg Phe Ser Gly Ala Leu

50 55 60

Ser Gly Ser Thr Ser Thr Leu Thr Ile Thr Gly Val Gln Ala Asp Asp

65 70 75 80

Glu Ala Val Tyr Phe Cys Gly Ser Ala Asp Ser Asn Thr Tyr Glu Gly

85 90 95

Ser Phe Gly Ala Gly Thr Thr Leu Thr Val Leu

100 105

<210> 70

<211> 148

<212> PRT

<213> 小鼠(Mus musculus)

<400> 70

Met Glu Trp Ser Gly Val Phe Ile Phe Leu Leu Ser Gly Thr Ala Gly

1 5 10 15

Val Leu Ser Glu Val Gln Leu His Gln Phe Gly Ala Glu Leu Val Lys

20 25 30

Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

Thr Asp Tyr Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu

50 55 60

Glu Trp Ile Gly Asp Ile Asn Pro Asn Tyr Asp Ser Thr Ser Tyr Asn

65 70 75 80

Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser

85 90 95

Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Thr Ala Val

100 105 110

Tyr Tyr Cys Ala Arg Ser Arg Ser Tyr Asp Tyr Glu Gly Phe Ala Tyr

115 120 125

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro

130 135 140

Pro Ser Val Tyr

145

<210> 71

<211> 105

<212> PRT

<213> 小鼠(Mus musculus)

<400> 71

Gly Leu Phe Cys Ser Val Glu Arg Cys His Tyr Gln Leu Gln Ser Ser

1 5 10 15

Gln Asn Leu Leu Ser Ile Val Asn Arg Tyr His Tyr Met Ser Gly Asn

20 25 30

Pro Pro Lys Leu Leu Val Tyr Pro Ala Leu Leu Ile Tyr Glu Ala Ser

35 40 45

Ile Thr Lys Ser Cys Val Pro Asp Arg Phe Thr Arg Ser Gly Ser Gly

50 55 60

Thr Asn Phe Thr Leu Thr Ile Asn Phe Val His Ala Asp Asp Leu Ile

65 70 75 80

Phe Tyr Tyr Cys Gln His Asn Arg Gly Ser Phe Leu Pro Ser Ser Ser

85 90 95

Val Gln Val Pro Arg Arg Arg Ser Asn

100 105

<210> 72

<211> 109

<212> PRT

<213> 小鼠(Mus musculus)

<400> 72

Pro Arg Ala Ser Leu Gly Val Ser Glu Thr Leu Leu Cys Thr Ser Gly

1 5 10 15

Phe Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Pro Pro Gly

20 25 30

Lys Ala Leu Glu Trp Leu Gly Phe Ile Arg Asn Lys Ala Asn Gly Tyr

35 40 45

Thr Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg

50 55 60

Asp Asn Ser Gln Ser Ile Leu Tyr Leu Gln Met Asn Thr Leu Arg Ala

65 70 75 80

Glu Asp Ser Ala Thr Tyr Tyr Cys Ala Arg Ala Asn Trp Ala Phe Asp

85 90 95

Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Lys

100 105

<210> 73

<211> 94

<212> PRT

<213> 小鼠(Mus musculus)

<400> 73

Ser Gly Asp Arg Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser

1 5 10 15

Asn Tyr Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu

20 25 30

Leu Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe

35 40 45

Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val

50 55 60

Glu Thr Glu Asp Phe Gly Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp

65 70 75 80

Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gln

85 90

<210> 74

<211> 118

<212> PRT

<213> 小鼠(Mus musculus)

<400> 74

Ala Ala Glu Leu Val Arg Pro Gly Thr Ser Val Lys Val Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Ala Phe Thr Asn Tyr Leu Ile Val Trp Ile Lys Gln

20 25 30

Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Ser Pro Gly Ser

35 40 45

Gly Gly Thr Asn Tyr Asn Glu Lys Phe Lys Gly Lys Ala Ile Leu Thr

50 55 60

Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr

65 70 75 80

Ser Asp Glu Phe Ala Val Tyr Phe Cys Ala Arg Glu Lys Ile Tyr Asp

85 90 95

Asp Tyr Tyr Glu Gly Tyr Phe Asp Val Trp Gly Ala Gly Pro Arg His

100 105 110

Leu Leu Ala Ser Leu Ser

115

<210> 75

<211> 107

<212> PRT

<213> 小鼠(Mus musculus)

<400> 75

Gly Thr Arg Cys Asp Ile Arg Leu Thr Gln Thr Thr Ser Ser Leu Ser

1 5 10 15

Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Leu Gly

20 25 30

Ile Gly Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val

35 40 45

Lys Leu Leu Ile Tyr Tyr Thr Ser Asn Leu His Ser Gly Val Pro Ser

50 55 60

Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser

65 70 75 80

Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln His Tyr Ser

85 90 95

Lys Leu Pro Leu Thr Phe Gly Ala Gly Pro Ser

100 105

<210> 76

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 76

Gly Ala Glu Leu Val Arg Ser Gly Ala Ser Val Lys Met Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Ser Phe Thr Asp Tyr Asn Met Tyr Trp Val Lys Gln

20 25 30

Thr Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Tyr Pro Gly Asn

35 40 45

Gly Gly Thr Asn Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr

50 55 60

Ala Asp Thr Ser Ser Ser Thr Ala Tyr Met Gln Ile Ser Ser Leu Thr

65 70 75 80

Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg Asp Tyr Asp Asp Gly

85 90 95

Gly Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser

100 105 110

Ser

<210> 77

<211> 117

<212> PRT

<213> 小鼠(Mus musculus)

<400> 77

Leu Leu Leu Trp Leu Thr Gly Ala Arg Cys Asp Ile Gln Met Thr Gln

1 5 10 15

Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr Val Thr Ile Thr

20 25 30

Cys Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Thr Trp Tyr Gln Gln

35 40 45

Lys Gln Gly Lys Ser Pro Gln Leu Leu Val Tyr Asn Ala Lys Thr Leu

50 55 60

Ala Asp Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln

65 70 75 80

Tyr Ser Leu Lys Ile Asn Arg Leu Gln Pro Glu Asp Phe Gly Ser Tyr

85 90 95

Tyr Cys Gln His Phe Trp Asn Ile Pro Trp Thr Phe Gly Gly Gly Thr

100 105 110

Lys Leu Asn Ser Arg

115

<210> 78

<211> 114

<212> PRT

<213> 小鼠(Mus musculus)

<400> 78

Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Thr Phe Thr Asp His Ser Ile His Trp Val Gln Gln

20 25 30

Lys Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr Ile Ser Pro Gly Asn

35 40 45

Gly Asn Ile Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr

50 55 60

Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr

65 70 75 80

Ser Glu Asp Ser Ala Val Tyr Phe Cys Lys Arg Ser Leu Gly Arg Gly

85 90 95

Gly Pro Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val

100 105 110

Ser Ser

<210> 79

<211> 108

<212> PRT

<213> 小鼠(Mus musculus)

<400> 79

Asp Ile Val Leu Thr Gln Ala Ala Pro Ser Leu Pro Val Thr Pro Gly

1 5 10 15

Glu Ser Val Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser

20 25 30

Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser

35 40 45

Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro

50 55 60

Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile

65 70 75 80

Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His

85 90 95

Arg Glu Tyr Pro Val Thr Phe Gly Ser Gly Pro Asn

100 105

<210> 80

<211> 111

<212> PRT

<213> 小鼠(Mus musculus)

<400> 80

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Met Lys Pro Gly Ala

1 5 10 15

Ser Val Lys Ile Ser Cys Lys Ala Thr Gly Tyr Thr Phe Ser Ser Tyr

20 25 30

Trp Ile Glu Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile

35 40 45

Gly Glu Ile Leu Pro Gly Ser Gly Ser Thr Asn Tyr Asn Glu Lys Phe

50 55 60

Lys Gly Lys Ala Thr Phe Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr

65 70 75 80

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys

85 90 95

Ala Ser Tyr Tyr Trp Tyr Phe Asp Val Trp Ala Gln Asp His Val

100 105 110

<210> 81

<211> 109

<212> PRT

<213> 小鼠(Mus musculus)

<400> 81

Ile Val Met Thr Gln Ala Ala Phe Ser Asn Pro Val Thr Leu Gly Thr

1 5 10 15

Ser Ala Ser Ile Ser Cys Arg Ser Ser Lys Asn Leu Leu His Ser Asn

20 25 30

Gly Ile Thr Tyr Leu Tyr Trp Tyr Leu Gln Arg Pro Gly Gln Ser Pro

35 40 45

Gln Leu Leu Ile Tyr Arg Val Ser Asn Leu Ala Ser Gly Val Pro Asn

50 55 60

Arg Phe Ser Gly Ser Glu Ser Gly Thr Asp Phe Thr Leu Arg Ile Ser

65 70 75 80

Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ala Gln Leu Leu

85 90 95

Glu Leu Pro Tyr Thr Ser Glu Gly Thr Lys Arg Trp Glu

100 105

<210> 82

<211> 109

<212> PRT

<213> 小鼠(Mus musculus)

<400> 82

Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala

1 5 10 15

Ala Ser Gly Phe Ala Phe Ser Ser Tyr Asp Met Ser Trp Ile Arg Gln

20 25 30

Thr Pro Glu Lys Arg Leu Glu Trp Val Ala Tyr Ile Ser Ser Gly Ala

35 40 45

Gly Ser Thr Tyr Tyr Pro Asp Thr Val Lys Gly Arg Phe Thr Val Ser

50 55 60

Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Ser Ser Leu Lys

65 70 75 80

Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Arg His Phe Tyr Arg Phe

85 90 95

Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser

100 105

<210> 83

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 83

Leu Leu Leu Cys Val Ser Gly Ala Pro Gly Ser Ile Val Met Thr Gln

1 5 10 15

Thr Pro Lys Phe Leu Leu Val Ser Ala Gly Asp Arg Ile Thr Ile Thr

20 25 30

Cys Lys Ala Ser Gln Ser Val Ser Asn Asp Val Ala Trp Tyr Gln Gln

35 40 45

Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Asn Arg

50 55 60

Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Tyr Gly Thr Asp

65 70 75 80

Phe Thr Phe Thr Ile Ser Thr Val Gln Ala Glu Asp Leu Ala Val Tyr

85 90 95

Phe Cys Gln Gln Asp Asp Arg Phe Pro Leu Thr Phe Gly Ala Gly Pro

100 105 110

Ser

<210> 84

<211> 112

<212> PRT

<213> 小鼠(Mus musculus)

<400> 84

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu

1 5 10 15

Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr

20 25 30

Gly Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Lys Trp Met

35 40 45

Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe

50 55 60

Lys Gly Arg Phe Ala Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr

65 70 75 80

Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Thr Ala Thr Tyr Phe Cys

85 90 95

Ala Thr Gly Ala Trp Phe Ala Tyr Trp Ala Lys Asp Ser Ser Arg His

100 105 110

<210> 85

<211> 107

<212> PRT

<213> 小鼠(Mus musculus)

<400> 85

Gly Val Glu Gly Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser

1 5 10 15

Thr Ser Val Gly Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp

20 25 30

Val Gly Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro

35 40 45

Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp

50 55 60

Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser

65 70 75 80

Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Ser

85 90 95

Ser Tyr Pro Leu Thr Phe Gly Ala Gly Pro Ser

100 105

<210> 86

<211> 118

<212> PRT

<213> 小鼠(Mus musculus)

<400> 86

Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met Lys Val Ser Cys Val

1 5 10 15

Ala Ser Gly Phe Ser Phe Ile Asp Phe Trp Met Asn Trp Val Arg Gln

20 25 30

Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Leu Lys Ser

35 40 45

Asn Asn Tyr Ala Thr His Tyr Ala Glu Ser Val Lys Gly Arg Phe Thr

50 55 60

Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Asn

65 70 75 80

Leu Arg Pro Glu Asp Thr Gly Ile Tyr Tyr Cys Thr Ser Leu Phe Tyr

85 90 95

Tyr Tyr Asp Gly Thr Ser Gly Phe Ala Tyr Trp Gly Gln Gly Thr Thr

100 105 110

Val Thr Val Leu Leu Lys

115

<210> 87

<211> 109

<212> PRT

<213> 小鼠(Mus musculus)

<400> 87

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly

1 5 10 15

Glu Lys Val Thr Met His Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser

20 25 30

Gly Asp Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln

35 40 45

Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val

50 55 60

Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr

65 70 75 80

Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn

85 90 95

Asp Tyr Asp Tyr Pro Leu Thr Phe Gly Ala Gly Pro Ser

100 105

<210> 88

<211> 148

<212> PRT

<213> 小鼠(Mus musculus)

<400> 88

Met Glu Trp Ser Gly Val Phe Ile Phe Leu Leu Ser Gly Thr Ala Gly

1 5 10 15

Val Leu Ser Glu Val Gln Leu His Gln Phe Gly Ala Glu Leu Val Lys

20 25 30

Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe

35 40 45

Thr Asp Tyr Asn Met Asp Trp Val Lys Gln Ser His Gly Lys Ser Leu

50 55 60

Glu Trp Ile Gly Asp Ile Asn Pro Asn Tyr Asp Ser Thr Ser Tyr Asn

65 70 75 80

Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser

85 90 95

Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Thr Ala Val

100 105 110

Tyr Tyr Cys Ala Arg Ser Arg Ser Tyr Asp Tyr Glu Gly Phe Ala Tyr

115 120 125

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ala Lys Thr Thr Pro

130 135 140

Pro Ser Val Tyr

145

<210> 89

<211> 139

<212> PRT

<213> 小鼠(Mus musculus)

<400> 89

Met Ser Val Leu Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr

1 5 10 15

Gly Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser

20 25 30

Ala Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn

35 40 45

Ile His Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro

50 55 60

Gln Leu Leu Val Tyr Asn Ala Lys Thr Leu Ala Asp Gly Val Pro Ser

65 70 75 80

Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn

85 90 95

Ser Leu Gln Pro Glu Asp Phe Gly Ser Tyr Tyr Cys Gln His Phe Trp

100 105 110

Ser Thr Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala

115 120 125

Asp Ala Ala Pro Thr Val Ser Asn Pro Tyr Asp

130 135

<210> 90

<211> 100

<212> PRT

<213> 小鼠(Mus musculus)

<400> 90

Asp Ile Leu Gln Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn

1 5 10 15

Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile

20 25 30

Asn Pro Tyr Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys Gly Lys

35 40 45

Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu

50 55 60

Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Trp

65 70 75 80

Gly Val Trp Ser Ala Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr

85 90 95

Val Ser Ser Lys

100

<210> 91

<211> 90

<212> PRT

<213> 小鼠(Mus musculus)

<400> 91

Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asn Val Arg Thr Ala

1 5 10 15

Val Ala Trp Tyr Gln Gln Lys Pro Arg Gln Ser Pro Lys Ala Leu Ile

20 25 30

Tyr Leu Ala Ser Asn Arg Asp Thr Gly Leu Pro Asp Arg Phe Pro Gly

35 40 45

Arg Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile Thr Asn Val Gln Ser

50 55 60

Glu Asp Leu Glu Asp Tyr Phe Cys Leu Gln His Cys Asn Tyr Pro Asn

65 70 75 80

Glu Phe Arg Gly Cys Thr Lys Val Pro Ile

85 90

<210> 92

<211> 116

<212> PRT

<213> 小鼠(Mus musculus)

<400> 92

Leu Gln Glu Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser Val Lys

1 5 10 15

Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met Gln

20 25 30

Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Ala Ile

35 40 45

Tyr Pro Gly Asp Gly Asp Thr Arg Tyr Thr Gln Lys Phe Lys Gly Lys

50 55 60

Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu

65 70 75 80

Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly

85 90 95

Glu Tyr Gly Asn Tyr Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr

100 105 110

Val Ser Ser Asn

115

<210> 93

<211> 100

<212> PRT

<213> 小鼠(Mus musculus)

<400> 93

Thr Ser Asp Ala Ser Leu Gly Glu Arg Val Thr Ile Thr Cys Lys Ala

1 5 10 15

Ser Gln Asp Ile Asn Ser Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly

20 25 30

Lys Ser Pro Lys Thr Leu Ile Tyr Arg Ala Asn Arg Leu Val Asp Gly

35 40 45

Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Gln Asp Tyr Ser Leu

50 55 60

Thr Ile Ser Ser Leu Glu Tyr Glu Asp Met Gly Ile Tyr Tyr Cys Leu

65 70 75 80

Gln Tyr Asp Glu Phe Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu

85 90 95

Ile Lys Gln Lys

100

<210> 94

<211> 108

<212> PRT

<213> 小鼠(Mus musculus)

<400> 94

Ala Trp Leu Ser Gln Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys

1 5 10 15

Asp Thr Tyr Met His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu

20 25 30

Trp Ile Gly Arg Ile Asp Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro

35 40 45

Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr

50 55 60

Ala Tyr Leu Gln Leu Ser Ser Leu Thr Ser Glu Asp Thr Ala Val Tyr

65 70 75 80

Tyr Cys Ala Arg Pro Ile His Tyr Tyr Tyr Gly Ser Ser Leu Ala Tyr

85 90 95

Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Lys

100 105

<210> 95

<211> 104

<212> PRT

<213> 小鼠(Mus musculus)

<400> 95

Glu Phe His Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg

1 5 10 15

Ala Ser Glu Ser Val Asp Ser Tyr Gly Asn Ser Phe Met His Trp Tyr

20 25 30

Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Arg Ala Ser

35 40 45

Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg

50 55 60

Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val Ala

65 70 75 80

Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Gly Arg Ser Glu Val

85 90 95

Val Pro Ser Trp Arg Ser Asn Lys

100

<210> 96

<211> 109

<212> PRT

<213> 小鼠(Mus musculus)

<400> 96

Pro Arg Ala Ser Leu Gly Val Ser Glu Thr Leu Leu Cys Thr Ser Gly

1 5 10 15

Phe Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Pro Pro Gly

20 25 30

Lys Ala Leu Glu Trp Leu Gly Phe Ile Arg Asn Lys Ala Asn Gly Tyr

35 40 45

Thr Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser Arg

50 55 60

Asp Asn Ser Gln Ser Ile Leu Tyr Leu Gln Met Asn Thr Leu Arg Ala

65 70 75 80

Glu Asp Ser Ala Thr Tyr Tyr Cys Ala Arg Ala Asn Trp Ala Phe Asp

85 90 95

Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Lys

100 105

<210> 97

<211> 94

<212> PRT

<213> 小鼠(Mus musculus)

<400> 97

Ser Gly Asp Arg Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser

1 5 10 15

Asn Tyr Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu

20 25 30

Leu Ile Lys Tyr Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe

35 40 45

Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val

50 55 60

Glu Thr Glu Asp Phe Gly Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp

65 70 75 80

Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gln

85 90

<210> 98

<211> 111

<212> PRT

<213> 小鼠(Mus musculus)

<400> 98

Pro Ala Cys Leu Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Thr Ser

1 5 10 15

Gly Phe Thr Phe Thr Asp Tyr Tyr Met Ser Trp Val Arg Gln Pro Pro

20 25 30

Gly Lys Ala Leu Glu Trp Leu Gly Phe Ile Arg Asn Lys Ala Asn Gly

35 40 45

Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Ile Ser

50 55 60

Arg Asp Asn Ser Gln Ser Ile Leu Tyr Leu Gln Met Asn Thr Leu Arg

65 70 75 80

Ala Glu Asp Ser Ala Thr Tyr Tyr Cys Ala Arg Ala Pro Leu Leu Tyr

85 90 95

Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser

100 105 110

<210> 99

<211> 102

<212> PRT

<213> 小鼠(Mus musculus)

<400> 99

Arg Leu Pro Phe Tyr Ser Leu Glu Gln Arg Ala Thr Ile Ser Tyr Arg

1 5 10 15

Ala Ser Lys Asn Val Ser Thr Ser Gly Tyr Ser Tyr Met His Trp Asn

20 25 30

Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Leu Val Ser

35 40 45

Asn Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly

50 55 60

Thr Asp Phe Thr Leu Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala

65 70 75 80

Thr Tyr Tyr Cys Gln His Ile Arg Glu Leu Thr Arg Ser Glu Leu Val

85 90 95

Pro Ser Trp Lys Ser Asn

100

<210> 100

<211> 101

<212> PRT

<213> 小鼠(Mus musculus)

<400> 100

Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met His

1 5 10 15

Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Met Ile

20 25 30

Asp Pro Ser Asn Ser Glu Thr Arg Leu Asn Gln Lys Phe Lys Asp Lys

35 40 45

Ala Thr Leu Asn Val Asp Lys Ser Ser Asn Thr Ala Tyr Met Gln Leu

50 55 60

Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly

65 70 75 80

Leu Arg His Tyr Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Thr Val

85 90 95

Thr Val Ser Ser Lys

100

<210> 101

<211> 99

<212> PRT

<213> 小鼠(Mus musculus)

<400> 101

Thr Ile Leu Trp Arg Glu Gly Pro Phe Ser Tyr Arg Ala Ser Lys Ser

1 5 10 15

Val Ser Thr Ser Gly Tyr Ser Tyr Met His Trp Asn Gln Gln Lys Pro

20 25 30

Gly Gln Pro Pro Arg Leu Leu Ile Tyr Leu Val Ser Asn Leu Glu Ser

35 40 45

Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr

50 55 60

Leu Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys

65 70 75 80

Gln His Ile Arg Glu Leu Thr Arg Ser Glu Glu Val Pro Ser Trp Arg

85 90 95

Ser Asn Lys

<210> 102

<211> 110

<212> PRT

<213> 小鼠(Mus musculus)

<400> 102

Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala

1 5 10 15

Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met Ser Trp Val Arg Gln

20 25 30

Thr Pro Glu Lys Arg Leu Glu Trp Val Ala Thr Ile Ser Ser Gly Gly

35 40 45

Ser Tyr Thr Tyr Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser

50 55 60

Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu Gln Met Ser Ser Leu Arg

65 70 75 80

Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala Ser Leu Ala Ser Tyr Tyr

85 90 95

Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser

100 105 110

<210> 103

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 103

Gly Ala Arg Cys Asp Val Gln Met Ile Gln Ser Pro Ser Ser Leu Ser

1 5 10 15

Ala Ser Leu Gly Asp Ile Val Thr Met Thr Cys Gln Ala Ser Gln Gly

20 25 30

Thr Ser Ile Asn Leu Asn Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro

35 40 45

Lys Leu Leu Ile Tyr Gly Ala Ser Ser Leu Glu Asp Gly Val Pro Ser

50 55 60

Arg Phe Ser Gly Ser Cys Phe Gly Thr Asp Phe Thr Leu Thr Ile Ser

65 70 75 80

Ser Leu Glu Asp Glu Asp Met Ala Thr Tyr Phe Cys Leu Gln His Ser

85 90 95

Tyr Leu Pro Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys

100 105 110

Arg

<210> 104

<211> 111

<212> PRT

<213> 小鼠(Mus musculus)

<400> 104

Gly Pro Gly Leu Val Gln Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr

1 5 10 15

Val Ser Gly Phe Ser Leu Thr Thr Tyr Asp Leu His Trp Val Arg Gln

20 25 30

Ser Pro Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ser Gly Gly

35 40 45

Ser Thr Asp Tyr Asn Ala Ala Phe Ile Ser Arg Leu Ser Ile Ser Lys

50 55 60

Asp Asn Ser Lys Ser Gln Val Phe Phe Lys Met Asn Ser Leu Gln Ala

65 70 75 80

Asn Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Asn Tyr Gly Tyr Ser Ala

85 90 95

Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala

100 105 110

<210> 105

<211> 118

<212> PRT

<213> 小鼠(Mus musculus)

<400> 105

Pro Ala Ser Ser Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu

1 5 10 15

Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln

20 25 30

Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln

35 40 45

Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg

50 55 60

Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp

65 70 75 80

Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr

85 90 95

Tyr Cys Phe Gln Gly Ser His Val Pro Leu Thr Phe Gly Ala Gly Thr

100 105 110

Lys Leu Glu Leu Lys Arg

115

<210> 106

<211> 114

<212> PRT

<213> 小鼠(Mus musculus)

<400> 106

Gly Phe Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Thr Phe Thr Ala Tyr Ser Met His Trp Val Lys Gln

20 25 30

Thr Pro Gly Lys Gly Leu Lys Trp Leu Gly Trp Ile Asn Thr Glu Thr

35 40 45

Gly Glu Pro Thr Tyr Thr Asp Asp Phe Lys Gly Arg Phe Thr Phe Ser

50 55 60

Leu Glu Thr Ser Ala Arg Ile Ala Tyr Leu Gln Ile Asn Asp Leu Lys

65 70 75 80

Asn Glu Asp Thr Ala Thr Tyr Phe Cys Ala Arg Arg Ile Tyr Tyr Phe

85 90 95

Gly Arg Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val

100 105 110

Ser Ser

<210> 107

<211> 118

<212> PRT

<213> 小鼠(Mus musculus)

<400> 107

Pro Ala Ser Ser Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu

1 5 10 15

Pro Val Arg Leu Gly Asp Gln Ser Ser Ile Ser Cys Arg Ser Ser Gln

20 25 30

Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln

35 40 45

Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg

50 55 60

Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp

65 70 75 80

Phe Thr Leu Lys Ile Ser Arg Val Glu Pro Glu Asp Leu Gly Val Tyr

85 90 95

Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr Ser Glu Gly Asp Gln

100 105 110

Ala Glu Ile Lys Leu Ala

115

<210> 108

<211> 114

<212> PRT

<213> 小鼠(Mus musculus)

<400> 108

Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Met Arg Leu Ser Cys Val

1 5 10 15

Ala Ser Gly Phe Thr Phe Ser Asn Ser Trp Phe Asn Trp Val Arg Gln

20 25 30

Ser Pro Glu Lys Gly Leu Glu Trp Val Ala Glu Ile Arg Leu Thr Ser

35 40 45

Asp Asn Tyr Ala Ile Tyr Tyr Ala Glu Ser Val Lys Gly Arg Phe Thr

50 55 60

Ile Ser Arg Asp Asp Ser Lys Ser Ser Val Tyr Leu Gln Met Asn Asn

65 70 75 80

Leu Arg Ala Glu Asp Thr Gly Ile Tyr Tyr Cys Thr Arg Pro Glu Thr

85 90 95

Ala Arg Ala Thr Phe Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val

100 105 110

Ser Ser

<210> 109

<211> 118

<212> PRT

<213> 小鼠(Mus musculus)

<400> 109

Pro Ala Ser Thr Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu

1 5 10 15

Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln

20 25 30

Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln

35 40 45

Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Lys Val Phe Asn Arg

50 55 60

Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp

65 70 75 80

Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr

85 90 95

Tyr Cys Phe Gln Gly Ser His Val Pro Arg Thr Phe Gly Gly Gly Thr

100 105 110

Lys Leu Asn Gln Thr Gly

115

<210> 110

<211> 111

<212> PRT

<213> 小鼠(Mus musculus)

<400> 110

Gly Pro Asp Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Ser Phe Thr Ala Tyr Tyr Met His Trp Val Lys Gln

20 25 30

Ser His Gly Lys Ser Leu Glu Trp Ile Gly Arg Val Asn Pro Asn Asn

35 40 45

Gly Gly Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala Ile Leu Thr

50 55 60

Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr

65 70 75 80

Phe Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Arg Ile Tyr Tyr Gly

85 90 95

Tyr Phe Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser

100 105 110

<210> 111

<211> 104

<212> PRT

<213> 小鼠(Mus musculus)

<400> 111

Ala Phe Phe Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Lys

1 5 10 15

Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Met Asn Trp Tyr

20 25 30

Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Val Ala Ser

35 40 45

Asn Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly

50 55 60

Thr Asp Phe Thr Leu Asn Ile His Pro Val Glu Glu Glu Asp Ala Ala

65 70 75 80

Thr Tyr Tyr Cys Gln Gln Ser Asn Glu Asp Pro Tyr Thr Phe Gly Gly

85 90 95

Gly Thr Lys Leu Glu Ile Lys Gln

100

<210> 112

<211> 106

<212> PRT

<213> 小鼠(Mus musculus)

<400> 112

Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys Thr

1 5 10 15

Ala Ser Gly Leu Asn Ile Arg Asp Ile Tyr Met His Trp Val Lys Gln

20 25 30

Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly Lys Ile Asp Pro Ala Asn

35 40 45

Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr

50 55 60

Ala Asp Thr Ser Ser Asn Thr Ala Tyr Val Gln Leu Ser Ser Leu Thr

65 70 75 80

Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Gly Thr Gly Asp Tyr Trp

85 90 95

Gly Gln Gly Thr Thr Val Thr Val Ser Ser

100 105

<210> 113

<211> 112

<212> PRT

<213> 小鼠(Mus musculus)

<400> 113

Gly Thr Cys Gly Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Ala

1 5 10 15

Val Ser Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser

20 25 30

Leu Leu Asn Ser Arg Thr Arg Lys Asn Tyr Leu Ala Trp Val Gln His

35 40 45

Lys Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr Trp Ala Ser Thr Arg

50 55 60

Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp

65 70 75 80

Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr

85 90 95

Tyr Cys Arg Gln Ser Tyr Asn Leu Val Thr Phe Gly Ala Gly Pro Ser

100 105 110

<210> 114

<211> 112

<212> PRT

<213> 小鼠(Mus musculus)

<400> 114

Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Val Met His Trp Val Lys Gln

20 25 30

Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn

35 40 45

Asp Gly Thr Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr

50 55 60

Ser Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr

65 70 75 80

Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Arg Tyr Tyr Tyr Gly

85 90 95

Ser Ser Gly Gly Tyr Phe Asp Val Trp Ala Gln Asp His Val Arg Thr

100 105 110

<210> 115

<211> 108

<212> PRT

<213> 小鼠(Mus musculus)

<400> 115

Asp Val Gln Ile Thr Gln Ser Pro Ser Tyr Leu Ala Ala Ser Pro Gly

1 5 10 15

Glu Thr Ile Thr Ile Asn Cys Arg Ala Ser Lys Ser Ile Ser Lys Tyr

20 25 30

Leu Ala Trp Tyr Gln Glu Lys Pro Gly Lys Thr Asn Lys Leu Leu Ile

35 40 45

Tyr Ser Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser Arg Phe Ser Gly

50 55 60

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro

65 70 75 80

Glu Asp Phe Ala Met Tyr Tyr Cys Gln Gln His Asn Glu Tyr Pro Tyr

85 90 95

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg

100 105

<210> 116

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 116

Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Phe Met Asn Trp Val Met Gln

20 25 30

Ser His Gly Lys Ser Leu Glu Trp Ile Gly Arg Ile Asn Pro Tyr Asn

35 40 45

Gly Asp Thr Phe Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr

50 55 60

Val Asp Lys Ser Ser Ser Thr Ala His Met Glu Leu Arg Ser Leu Ala

65 70 75 80

Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Arg Ile His Tyr Tyr

85 90 95

Tyr Gly Ser Ser Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Glu Pro His

100 105 110

His

<210> 117

<211> 108

<212> PRT

<213> 小鼠(Mus musculus)

<400> 117

Asp Val Gln Ile Thr Gln Ser Pro Ser Tyr Leu Ala Ala Ser Pro Gly

1 5 10 15

Glu Thr Ile Thr Ile Asn Cys Arg Ala Ser Lys Ser Ile Ser Lys Tyr

20 25 30

Leu Ala Trp Tyr Gln Glu Lys Pro Gly Lys Thr Asn Lys Leu Leu Ile

35 40 45

Tyr Ser Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser Arg Phe Ser Gly

50 55 60

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro

65 70 75 80

Glu Asp Phe Ala Met Tyr Tyr Cys Gln Gln His Asn Glu Tyr Pro Trp

85 90 95

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg

100 105

<210> 118

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 118

Gly Ala Gly Leu Val Lys Pro Gly Ala Ser Val Lys Leu Ser Cys Lys

1 5 10 15

Ala Ser Gly Tyr Thr Phe Thr Glu Tyr Ile Ile His Trp Val Lys Gln

20 25 30

Arg Ser Gly Gln Gly Leu Glu Trp Ile Gly Trp Phe Tyr Pro Gly Ser

35 40 45

Gly Ser Ile Lys Tyr Asn Glu Lys Phe Lys Asp Lys Ala Thr Leu Thr

50 55 60

Ala Asp Lys Ser Ser Ser Thr Val Tyr Met Glu Leu Ser Arg Leu Thr

65 70 75 80

Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg His Glu Val Tyr Tyr

85 90 95

Asp Tyr Asp Lys Ser Met Leu Trp Thr Thr Gly Val Lys Asn Leu Ile

100 105 110

Arg

<210> 119

<211> 108

<212> PRT

<213> 小鼠(Mus musculus)

<400> 119

Ser Pro Ser Ser Leu Ala Val Ser Val Gly Glu Lys Val Thr Met Ser

1 5 10 15

Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser Ser Asn Gln Lys Asn Tyr

20 25 30

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile

35 40 45

Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly

50 55 60

Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Lys Ala

65 70 75 80

Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Tyr

85 90 95

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg

100 105

<210> 120

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 120

Gly Ala Glu Leu Val Arg Pro Gly Thr Ser Val Lys Val Ser Cys Lys

1 5 10 15

Ala Ser Val Tyr Ala Phe Thr Asn Tyr Leu Ile Glu Trp Val Lys Gln

20 25 30

Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Val Ile Asn Pro Lys Ser

35 40 45

Gly Gly Thr Lys Tyr Asn Glu Lys Phe Arg Gly Lys Ala Thr Leu Thr

50 55 60

Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Ser Ser Leu Thr

65 70 75 80

Ser Gly Asp Ser Ala Val Tyr Phe Cys Ala Ile Thr Gly Thr Asp Tyr

85 90 95

Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro

100 105 110

Pro

<210> 121

<211> 113

<212> PRT

<213> 小鼠(Mus musculus)

<400> 121

Gln Gly Thr Arg Cys Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu

1 5 10 15

Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln

20 25 30

Gly Ile Asn Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr

35 40 45

Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu Arg Ser Gly Val Pro

50 55 60

Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile

65 70 75 80

Ser Asn Leu Glu Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Tyr

85 90 95

Ser Lys Leu Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys

100 105 110

Arg

<210> 122

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 122

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 123

<211> 112

<212> PRT

<213> 人(Homo sapiens)

<400> 123

Gln Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp

1 5 10 15

Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn Glu

20 25 30

Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg Leu

35 40 45

Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe Ser

50 55 60

Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln

65 70 75 80

Cys Glu Asp Phe Ala Ile Tyr Tyr Cys Leu Gly Glu Phe Ser Cys Gly

85 90 95

Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys

100 105 110

<210> 124

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 124

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 125

<211> 112

<212> PRT

<213> 人(Homo sapiens)

<400> 125

Gln Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp

1 5 10 15

Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn Glu

20 25 30

Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg Leu

35 40 45

Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe Ser

50 55 60

Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln

65 70 75 80

Cys Glu Asp Phe Ala Ile Tyr Tyr Cys Leu Gly Glu Phe Ser Cys Gly

85 90 95

Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile Lys

100 105 110

<210> 126

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 126

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 127

<211> 113

<212> PRT

<213> 人(Homo sapiens)

<400> 127

Glu Gln Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly

1 5 10 15

Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn

20 25 30

Glu Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg

35 40 45

Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe

50 55 60

Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asn Leu

65 70 75 80

Gln Pro Glu Asp Phe Ala Ile Tyr Tyr Cys Leu Gly Glu Phe Ser Cys

85 90 95

Gly Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile

100 105 110

Lys

<210> 128

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 128

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 129

<211> 113

<212> PRT

<213> 人(Homo sapiens)

<400> 129

Glu Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly

1 5 10 15

Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn

20 25 30

Glu Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg

35 40 45

Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe

50 55 60

Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asn Leu

65 70 75 80

Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gly Glu Phe Ser Cys

85 90 95

Gly Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile

100 105 110

Lys

<210> 130

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 130

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 131

<211> 113

<212> PRT

<213> 人(Homo sapiens)

<400> 131

Glu Ile Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly

1 5 10 15

Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn

20 25 30

Glu Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg

35 40 45

Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe

50 55 60

Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asn Leu

65 70 75 80

Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gly Glu Phe Ser Cys

85 90 95

Gly Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile

100 105 110

Lys

<210> 132

<211> 121

<212> PRT

<213> 人(Homo sapiens)

<400> 132

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly

1 5 10 15

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Ser Leu Ser Ser His

20 25 30

Ser Leu Gly Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile

35 40 45

Gly Asp Ile Arg Ser Gly Gly Ser Ala Tyr Tyr Ala Asn Trp Ala Lys

50 55 60

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu

65 70 75 80

Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Thr

85 90 95

Arg Thr Asn Gly Pro Ser Asp Leu Thr Asn Arg Leu Asp Leu Trp Gly

100 105 110

Gln Gly Thr Leu Val Thr Val Ser Ser

115 120

<210> 133

<211> 113

<212> PRT

<213> 人(Homo sapiens)

<400> 133

Glu Gln Val Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly

1 5 10 15

Asp Arg Val Thr Ile Asn Cys Gln Ala Ser Gln Ser Leu Tyr Asn Asn

20 25 30

Glu Asn Leu Ala Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys Arg

35 40 45

Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val Ser Ser Arg Phe

50 55 60

Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asn Leu

65 70 75 80

Gln Pro Glu Asp Phe Ala Ile Tyr Tyr Cys Leu Gly Glu Phe Ser Cys

85 90 95

Gly Ser Ala Asp Cys Phe Ala Phe Gly Gly Gly Thr Lys Val Glu Ile

100 105 110

Lys

<210> 134

<211> 5

<212> PRT

<213> 鸡(Gallus gallus)

<400> 134

Gly Tyr Asp Met Leu

1 5

<210> 135

<211> 17

<212> PRT

<213> 鸡(Gallus gallus)

<400> 135

Gly Ile Gly Ser Thr Gly Gly Gly Thr Asp Tyr Gly Ala Ala Val Lys

1 5 10 15

Gly

<210> 136

<211> 19

<212> PRT

<213> 鸡(Gallus gallus)

<400> 136

Val Ala Gly Gly Cys Asn Ser Gly Tyr Cys Arg Asp Ser Pro Gly Ser

1 5 10 15

Ile Asp Ala

<210> 137

<211> 10

<212> PRT

<213> 鸡(Gallus gallus)

<400> 137

Ser Gly Gly Gly Ser Arg Asn Tyr Tyr Gly

1 5 10

<210> 138

<211> 7

<212> PRT

<213> 鸡(Gallus gallus)

<400> 138

Asp Asp Gln Arg Pro Ser Asn

1 5

<210> 139

<211> 11

<212> PRT

<213> 鸡(Gallus gallus)

<400> 139

Ser Ala Asp Ser Asn Thr Tyr Glu Gly Ser Phe

1 5 10

<210> 140

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 140

Asp Tyr Asn Met Asp

1 5

<210> 141

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 141

Asp Ile Asn Pro Asn Tyr Asp Ser Thr Ser Tyr Asn Gln Lys Phe Lys

1 5 10 15

Gly

<210> 142

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 142

Ser Arg Ser Tyr Asp Tyr Glu Gly Phe Ala Tyr

1 5 10

<210> 143

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 143

Leu Ser Ile Val Asn Arg Tyr His Tyr Met Ser

1 5 10

<210> 144

<211> 6

<212> PRT

<213> 小鼠(Mus musculus)

<400> 144

Glu Ala Ser Ile Thr Lys

1 5

<210> 145

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 145

Gln His Asn Arg Gly Ser Phe Leu Pro

1 5

<210> 146

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 146

Asp Tyr Tyr Met Ser

1 5

<210> 147

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 147

Arg Asn Lys Ala Asn Gly Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys

1 5 10 15

Gly

<210> 148

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 148

Ala Arg Ala Asn Trp Ala Phe Asp Tyr

1 5

<210> 149

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 149

Arg Ala Ser Gln Ser Ile Ser Asn Tyr Leu His

1 5 10

<210> 150

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 150

Tyr Ala Ser Gln Ser Ile Ser

1 5

<210> 151

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 151

Tyr Ala Ser Gln Ser Ile Ser

1 5

<210> 152

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 152

Asn Tyr Leu Ile Val

1 5

<210> 153

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 153

Val Ile Ser Pro Gly Ser Gly Gly Thr Asn Tyr Asn Glu Lys Phe Lys

1 5 10 15

Gly

<210> 154

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 154

Glu Lys Ile Tyr Asp Asp Tyr Tyr Glu Gly Tyr

1 5 10

<210> 155

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 155

Thr Ile Ser Cys Ser Ala Ser Leu Gly Ile Gly Asn Tyr Leu Asn

1 5 10 15

<210> 156

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 156

Thr Ser Asn Leu His Ser Gly

1 5

<210> 157

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 157

His Tyr Ser Lys Leu Pro Leu Thr Phe

1 5

<210> 158

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 158

Asp Tyr Asn Met Tyr

1 5

<210> 159

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 159

Tyr Ile Tyr Pro Gly Asn Gly Gly Thr Asn Tyr Asn Gln Lys Phe Lys

1 5 10 15

Gly

<210> 160

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 160

Asp Tyr Asp Asp Gly Gly Tyr Ala Met Asp Tyr

1 5 10

<210> 161

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 161

Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Gly Asn

1 5 10 15

<210> 162

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 162

Asn Ala Lys Thr Leu Ala Asp

1 5

<210> 163

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 163

Gln His Phe Trp Asn Ile Pro Trp Thr

1 5

<210> 164

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 164

Asp His Ser Ile His

1 5

<210> 165

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 165

Tyr Ile Ser Pro Gly Asn Gly Asn Ile Lys Tyr Asn Glu Lys Phe Lys

1 5 10 15

Gly

<210> 166

<211> 12

<212> PRT

<213> 小鼠(Mus musculus)

<400> 166

Ser Leu Gly Arg Gly Gly Pro Tyr Tyr Phe Asp Tyr

1 5 10

<210> 167

<211> 16

<212> PRT

<213> 小鼠(Mus musculus)

<400> 167

Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu Tyr

1 5 10 15

<210> 168

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 168

Arg Met Ser Asn Leu Ala Ser

1 5

<210> 169

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 169

Met Gln His Arg Glu Tyr Pro Val Thr

1 5

<210> 170

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 170

Ser Tyr Trp Ile Glu

1 5

<210> 171

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 171

Glu Ile Leu Pro Gly Ser Gly Ser Thr Asn Tyr Asn Glu Lys Phe Lys

1 5 10 15

Gly

<210> 172

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 172

Tyr Tyr Trp Tyr Phe Asp Val Trp Ala Gln Asp

1 5 10

<210> 173

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 173

Ser Ser Lys Asn Leu Leu His Ser Asn Gly Ile Thr Tyr Leu Tyr

1 5 10 15

<210> 174

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 174

Arg Val Ser Asn Leu Ala Ser

1 5

<210> 175

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 175

Ala Gln Leu Leu Glu Leu Pro Tyr Thr

1 5

<210> 176

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 176

Ser Tyr Asp Met Ser

1 5

<210> 177

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 177

Tyr Ile Ser Ser Gly Ala Gly Ser Thr Tyr Tyr Pro Asp Thr Val Lys

1 5 10 15

Gly

<210> 178

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 178

His Phe Tyr Arg Phe Asp Tyr Trp Gly Gln Gly

1 5 10

<210> 179

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 179

Ser Ala Gly Asp Arg Ile Thr Ile Thr Cys Lys Ala Ser Gln Ser

1 5 10 15

<210> 180

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 180

Tyr Ala Ser Asn Arg Tyr Thr

1 5

<210> 181

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 181

Gln Gln Asp Asp Arg Phe Pro Leu Thr

1 5

<210> 182

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 182

Asn Tyr Gly Met Asn

1 5

<210> 183

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 183

Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Asp Asp Phe Lys

1 5 10 15

Gly

<210> 184

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 184

Gly Ala Trp Phe Ala Tyr Trp Ala Lys Asp Ser

1 5 10

<210> 185

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 185

Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Thr Ala Val Ala

1 5 10 15

<210> 186

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 186

Trp Ala Ser Thr Arg His Thr

1 5

<210> 187

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 187

Gln Gln Tyr Ser Ser Tyr Pro Leu Thr

1 5

<210> 188

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 188

Asp Phe Trp Met Asn

1 5

<210> 189

<211> 19

<212> PRT

<213> 小鼠(Mus musculus)

<400> 189

Glu Ile Arg Leu Lys Ser Asn Asn Tyr Ala Thr His Tyr Ala Glu Ser

1 5 10 15

Val Lys Gly

<210> 190

<211> 13

<212> PRT

<213> 小鼠(Mus musculus)

<400> 190

Leu Phe Tyr Tyr Tyr Asp Gly Thr Ser Gly Phe Ala Tyr

1 5 10

<210> 191

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 191

Lys Ser Ser Gln Ser Leu Leu Asn Ser Gly Asp Gln Lys Asn Tyr Leu

1 5 10 15

Thr

<210> 192

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 192

Trp Ala Ser Thr Arg Glu Ser

1 5

<210> 193

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 193

Gln Asn Asp Tyr Asp Tyr Pro Leu Thr

1 5

<210> 194

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 194

Asp Tyr Asn Met Asp

1 5

<210> 195

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 195

Asp Ile Asn Pro Asn Tyr Asp Ser Thr Ser Tyr Asn Gln Lys Phe Lys

1 5 10 15

Gly

<210> 196

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 196

Ser Arg Ser Tyr Asp Tyr Glu Gly Phe Ala Tyr

1 5 10

<210> 197

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 197

Arg Ala Ser Gly Asn Ile His Asn Tyr Leu Ala

1 5 10

<210> 198

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 198

Asn Ala Lys Thr Leu Ala Asp

1 5

<210> 199

<211> 8

<212> PRT

<213> 小鼠(Mus musculus)

<400> 199

Gln His Phe Trp Ser Thr Leu Thr

1 5

<210> 200

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 200

Gly Tyr Thr Met Asn

1 5

<210> 201

<211> 16

<212> PRT

<213> 小鼠(Mus musculus)

<400> 201

Asn Pro Tyr Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe Lys Gly Lys

1 5 10 15

<210> 202

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 202

Trp Gly Val Trp Ser Ala Met Asp Tyr

1 5

<210> 203

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 203

Lys Ala Ser Gln Asn Val Arg Thr Ala Val Ala

1 5 10

<210> 204

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 204

Leu Ala Ser Asn Arg Asp Thr

1 5

<210> 205

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 205

Leu Gln His Cys Asn Tyr Pro Asn Glu

1 5

<210> 206

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 206

Ser Tyr Trp Met Gln

1 5

<210> 207

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 207

Ala Ile Tyr Pro Gly Asp Gly Asp Thr Arg Tyr Thr Gln Lys Phe Lys

1 5 10 15

Gly

<210> 208

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 208

Ala Arg Gly Glu Tyr Gly Asn Tyr Phe Ala Tyr

1 5 10

<210> 209

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 209

Lys Ala Ser Gln Asp Ile Asn Ser Tyr Leu Ser

1 5 10

<210> 210

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 210

Arg Ala Asn Arg Leu Val Asp

1 5

<210> 211

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 211

Leu Gln Tyr Asp Glu Phe Pro Leu Thr

1 5

<210> 212

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 212

Asp Thr Tyr Met His

1 5

<210> 213

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 213

Arg Ile Asp Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln

1 5 10 15

Gly

<210> 214

<211> 14

<212> PRT

<213> 小鼠(Mus musculus)

<400> 214

Ala Arg Pro Ile His Tyr Tyr Tyr Gly Ser Ser Leu Ala Tyr

1 5 10

<210> 215

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 215

Ser Val Asp Ser Tyr Gly Asn Ser Phe Met His

1 5 10

<210> 216

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 216

Arg Ala Ser Asn Leu Glu Ser

1 5

<210> 217

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 217

Gln Gln Ser Asn Glu Asp Pro Gly Arg

1 5

<210> 218

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 218

Asp Tyr Tyr Met Ser

1 5

<210> 219

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 219

Arg Asn Lys Ala Asn Gly Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys

1 5 10 15

Gly

<210> 220

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 220

Ala Arg Ala Asn Trp Ala Phe Asp Tyr

1 5

<210> 221

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 221

Arg Ala Ser Gln Ser Ile Ser Asn Tyr Leu His

1 5 10

<210> 222

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 222

Tyr Ala Ser Gln Ser Ile Ser

1 5

<210> 223

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 223

Gln Gln Ser Asn Ser Trp Pro Tyr Thr

1 5

<210> 224

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 224

Asp Tyr Tyr Met Ser

1 5

<210> 225

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 225

Arg Asn Lys Ala Asn Gly Tyr Thr Thr Glu Tyr Ser Ala Ser Val Lys

1 5 10 15

Gly

<210> 226

<211> 12

<212> PRT

<213> 小鼠(Mus musculus)

<400> 226

Ala Arg Ala Pro Leu Leu Tyr Tyr Ala Met Asp Tyr

1 5 10

<210> 227

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 227

Asn Val Ser Thr Ser Gly Tyr Ser Tyr Met His

1 5 10

<210> 228

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 228

Leu Val Ser Asn Leu Glu Ser

1 5

<210> 229

<211> 8

<212> PRT

<213> 小鼠(Mus musculus)

<400> 229

Gln His Ile Arg Glu Leu Thr Arg

1 5

<210> 230

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 230

Ser Tyr Trp Met His

1 5

<210> 231

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 231

Met Ile Asp Pro Ser Asn Ser Glu Thr Arg Leu Asn Gln Lys Phe Lys

1 5 10 15

Asp

<210> 232

<211> 12

<212> PRT

<213> 小鼠(Mus musculus)

<400> 232

Ala Arg Gly Leu Arg His Tyr Trp Tyr Phe Asp Val

1 5 10

<210> 233

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 233

Ser Val Ser Thr Ser Gly Tyr Ser Tyr Met His

1 5 10

<210> 234

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 234

Leu Val Ser Asn Leu Glu Ser

1 5

<210> 235

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 235

Gln His Ile Arg Glu Leu Thr Arg Ser

1 5

<210> 236

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 236

Ser Tyr Gly Met Ser

1 5

<210> 237

<211> 14

<212> PRT

<213> 小鼠(Mus musculus)

<400> 237

Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val Ala

1 5 10

<210> 238

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 238

Leu Ala Ser Tyr Tyr Phe Asp Tyr Trp Gly Gln

1 5 10

<210> 239

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 239

Thr Met Thr Cys Gln Ala Ser Gln Gly Thr Ser Ile Asn Leu Asn

1 5 10 15

<210> 240

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 240

Gly Ala Ser Ser Leu Glu Asp

1 5

<210> 241

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 241

Leu Gln His Ser Tyr Leu Pro Pro Leu Thr Phe

1 5 10

<210> 242

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 242

Thr Tyr Asp Leu His

1 5

<210> 243

<211> 16

<212> PRT

<213> 小鼠(Mus musculus)

<400> 243

Val Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile Ser

1 5 10 15

<210> 244

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 244

Asn Tyr Gly Tyr Ser Ala Trp Phe Ala Tyr Trp

1 5 10

<210> 245

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 245

Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser Asn

1 5 10 15

<210> 246

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 246

Lys Val Ser Asn Arg Phe Ser

1 5

<210> 247

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 247

Phe Gln Gly Ser His Val Pro Leu Thr

1 5

<210> 248

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 248

Ala Tyr Ser Met His

1 5

<210> 249

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 249

Trp Ile Asn Thr Glu Thr Gly Glu Pro Thr Tyr Thr Asp Asp Phe Lys

1 5 10 15

Gly

<210> 250

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 250

Arg Ile Tyr Tyr Phe Gly Arg Gly Gly Phe Asp

1 5 10

<210> 251

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 251

Ser Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser Asn

1 5 10 15

<210> 252

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 252

Lys Val Ser Asn Arg Phe Ser

1 5

<210> 253

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 253

Phe Gln Gly Ser His Val Pro Tyr Thr

1 5

<210> 254

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 254

Asn Ser Trp Phe Asn

1 5

<210> 255

<211> 19

<212> PRT

<213> 小鼠(Mus musculus)

<400> 255

Glu Ile Arg Leu Thr Ser Asp Asn Tyr Ala Ile Tyr Tyr Ala Glu Ser

1 5 10 15

Val Lys Gly

<210> 256

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 256

Pro Glu Thr Ala Arg Ala Thr Phe Ala Tyr Trp

1 5 10

<210> 257

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 257

Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser Asn

1 5 10 15

<210> 258

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 258

Lys Val Phe Asn Arg Phe Ser

1 5

<210> 259

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 259

Phe Gln Gly Ser His Val Pro Arg Thr

1 5

<210> 260

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 260

Ala Tyr Tyr Met His

1 5

<210> 261

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 261

Arg Val Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe Lys

1 5 10 15

Gly

<210> 262

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 262

Arg Ile Tyr Tyr Gly Tyr Phe Asp Tyr Trp Gly

1 5 10

<210> 263

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 263

Lys Ala Ser Gln Ser Val Asp Tyr Asp Gly Asp Ser Tyr Met Asn

1 5 10 15

<210> 264

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 264

Val Ala Ser Asn Leu Glu Ser

1 5

<210> 265

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 265

Gln Gln Ser Asn Glu Asp Pro Tyr Thr

1 5

<210> 266

<211> 4

<212> PRT

<213> 小鼠(Mus musculus)

<400> 266

Asp Ile Tyr Met

1

<210> 267

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 267

Lys Ile Asp Pro Ala Asn Gly Asn Thr Lys Tyr Asp Pro Lys Phe Gln

1 5 10 15

Gly

<210> 268

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 268

Thr Gly Asp Tyr Trp Gly Gln Gly Thr Thr Val

1 5 10

<210> 269

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 269

Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser Arg Thr

1 5 10 15

<210> 270

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 270

Trp Ala Ser Thr Arg Glu Ser

1 5

<210> 271

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 271

Arg Gln Ser Tyr Asn Leu Val Thr Phe

1 5

<210> 272

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 272

Ser Tyr Val Met His

1 5

<210> 273

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 273

Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe Lys

1 5 10 15

Gly

<210> 274

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 274

Arg Tyr Tyr Tyr Gly Ser Ser Gly Gly Tyr Phe

1 5 10

<210> 275

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 275

Arg Ala Ser Lys Ser Ile Ser Lys Tyr Leu Ala Trp Tyr Gln Glu

1 5 10 15

<210> 276

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 276

Ser Gly Ser Thr Leu Gln Ser

1 5

<210> 277

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 277

Gln Gln His Asn Glu Tyr Pro Tyr Thr

1 5

<210> 278

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 278

Gly Tyr Phe Met Asn

1 5

<210> 279

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 279

Arg Ile Asn Pro Tyr Asn Gly Asp Thr Phe Tyr Asn Gln Lys Phe Lys

1 5 10 15

Gly

<210> 280

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 280

Arg Ile His Tyr Tyr Tyr Gly Ser Ser Tyr Tyr

1 5 10

<210> 281

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 281

Arg Ala Ser Lys Ser Ile Ser Lys Tyr Leu Ala Trp Tyr Gln Glu

1 5 10 15

<210> 282

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 282

Ser Gly Ser Thr Leu Gln Ser

1 5

<210> 283

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 283

Gln Gln His Asn Glu Tyr Pro Trp Thr

1 5

<210> 284

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 284

Glu Tyr Ile Ile His

1 5

<210> 285

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 285

Trp Phe Tyr Pro Gly Ser Gly Ser Ile Lys Tyr Asn Glu Lys Phe Lys

1 5 10 15

Asp

<210> 286

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 286

His Glu Val Tyr Tyr Asp Tyr Asp Lys Ser Met

1 5 10

<210> 287

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 287

Leu Tyr Ser Ser Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln

1 5 10 15

<210> 288

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 288

Trp Ala Ser Thr Arg Glu Ser

1 5

<210> 289

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 289

Gln Gln Tyr Tyr Ser Tyr Pro Tyr Thr

1 5

<210> 290

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 290

Asn Tyr Leu Ile Glu

1 5

<210> 291

<211> 19

<212> PRT

<213> 小鼠(Mus musculus)

<400> 291

Val Ile Asn Pro Lys Ser Gly Gly Thr Lys Tyr Asn Glu Lys Phe Arg

1 5 10 15

Gly Lys Ala

<210> 292

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 292

Thr Gly Thr Asp Tyr Trp Gly Gln Gly Thr Thr

1 5 10

<210> 293

<211> 10

<212> PRT

<213> 小鼠(Mus musculus)

<400> 293

Val Thr Ile Ser Cys Ser Ala Ser Gln Gly

1 5 10

<210> 294

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 294

Tyr Thr Ser Ser Leu Arg Ser

1 5

<210> 295

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 295

Gln Gln Tyr Ser Lys Leu Pro Arg Thr

1 5

<210> 296

<211> 12

<212> PRT

<213> 人(Homo sapiens)

<400> 296

Phe Thr Ser Gly Glu Lys Glu Gln Val Asp Glu Trp

1 5 10

<210> 297

<211> 16

<212> PRT

<213> 人(Homo sapiens)

<400> 297

Ala Glu Gln Lys Arg Leu Lys Thr Val Leu Glu Leu Gln Tyr Val Leu

1 5 10 15

<210> 298

<211> 16

<212> PRT

<213> 人(Homo sapiens)

<400> 298

Val Glu Arg Val Phe Gln Ser Asn Tyr Phe Asp Ser Thr His Asn His

1 5 10 15

<210> 299

<211> 16

<212> PRT

<213> 人(Homo sapiens)

<400> 299

Phe Gln Ser Met Gln Thr Val Phe Asn Met Asn Ala Pro Val Pro Pro

1 5 10 15

<210> 300

<211> 5

<212> PRT

<213> 小鼠(Mus musculus)

<400> 300

Thr Asn Ala Met Asn

1 5

<210> 301

<211> 17

<212> PRT

<213> 小鼠(Mus musculus)

<400> 301

Arg Ile Arg Ser Lys Ser Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser

1 5 10 15

Val

<210> 302

<211> 11

<212> PRT

<213> 小鼠(Mus musculus)

<400> 302

Asp Trp Asp Gly Phe Leu Tyr Phe Asp Tyr Trp

1 5 10

<210> 303

<211> 112

<212> PRT

<213> 小鼠(Mus musculus)

<400> 303

Gly Gly Gly Leu Val Gln Pro Lys Gly Ser Leu Lys Leu Ser Cys Ala

1 5 10 15

Ala Ser Gly Phe Thr Phe Asn Thr Asn Ala Met Asn Trp Val Arg Gln

20 25 30

Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Arg Ser Lys Ser

35 40 45

Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser Val Lys Asp Arg Phe Thr

50 55 60

Ile Ser Arg Asp Asp Ser Gln Ser Met Leu Tyr Leu Gln Met Asn Asn

65 70 75 80

Leu Lys Thr Glu Asp Thr Ala Met Tyr Tyr Cys Val Arg Asp Trp Asp

85 90 95

Gly Phe Leu Tyr Phe Asp Tyr Trp Ala Lys His His Leu Thr Leu Phe

100 105 110

<210> 304

<211> 15

<212> PRT

<213> 小鼠(Mus musculus)

<400> 304

Ser Val Ser Thr Ser Gly Tyr Ser Tyr Met His Trp Asn Gln Gln

1 5 10 15

<210> 305

<211> 7

<212> PRT

<213> 小鼠(Mus musculus)

<400> 305

Leu Val Ser Asn Leu Glu Ser

1 5

<210> 306

<211> 9

<212> PRT

<213> 小鼠(Mus musculus)

<400> 306

Gln His Ile Arg Glu Leu Thr Arg Ser

1 5

<210> 307

<211> 104

<212> PRT

<213> 小鼠(Mus musculus)

<400> 307

Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr

1 5 10 15

Ile Ser Tyr Arg Ala Ser Lys Ser Val Ser Thr Ser Gly Tyr Ser Tyr

20 25 30

Met His Trp Asn Gln Gln Lys Pro Gly Gln Pro Pro Arg Leu Leu Ile

35 40 45

Tyr Leu Val Ser Asn Leu Glu Ser Gly Val Pro Ala Arg Phe Ser Gly

50 55 60

Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His Pro Val Glu Glu

65 70 75 80

Glu Asp Ala Ala Thr Tyr Tyr Cys Gln His Ile Arg Glu Leu Thr Arg

85 90 95

Ser Glu Gly Gly Pro Ser Trp Lys

100

<210> 308

<211> 14

<212> PRT

<213> 人(Homo sapiens)

<400> 308

Pro Pro Val Asn Glu Pro Glu Thr Leu Lys Gln Gln Asn Gln

1 5 10

<210> 309

<211> 12

<212> PRT

<213> 人(Homo sapiens)

<400> 309

Glu Thr Leu Lys Gln Gln Asn Gln Tyr Gln Ala Ser

1 5 10

Claims (14)

1.用于癌的治疗和/或预防的药品，其特征在于，一起包含或各自分开地组合包含：与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段、和拓扑异构酶I抑制剂。

2.根据权利要求1所述的药品，所述拓扑异构酶I抑制剂是拓扑替康或伊立替康。

3.根据权利要求1或2所述的药品，所述抗体或其片段与CAPRIN-1蛋白质具有免疫反应性，所述CAPRIN-1蛋白质具有序列号2～30中任一偶数序列号所示的氨基酸序列、或与该氨基酸序列有80％以上的序列同一性的氨基酸序列。

4.根据权利要求1～3的任一项所述的药品，所述抗体或其片段与在癌细胞表面上存在的CAPRIN-1蛋白质的细胞外区域具有免疫反应性。

5.根据权利要求1～4的任一项所述的药品，所述抗体或其片段与CAPRIN-1蛋白质的部分多肽具有免疫反应性，所述CAPRIN-1蛋白质的部分多肽具有序列号31～35、296～299、308、309的任一项所示的氨基酸序列、或与该氨基酸序列有80％以上的序列同一性的氨基酸序列。

6.根据权利要求1～5的任一项所述的药品，所述抗体是单克隆抗体或多克隆抗体。

7.根据权利要求1～6的任一项所述的药品，所述抗体或其片段是以下(A)～(M)的任一者，

(A)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号36所示的互补决定区CDR1、序列号37所示的互补决定区CDR2、和序列号38所示的互补决定区CDR3，所述轻链可变区包含序列号40所示的互补决定区CDR1、序列号41所示的互补决定区CDR2、和序列号42所示的互补决定区CDR3；

(B)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号44所示的互补决定区CDR1、序列号45所示的互补决定区CDR2、和序列号46所示的互补决定区CDR3，所述轻链可变区包含序列号48所示的互补决定区CDR1、序列号49所示的互补决定区CDR2、和序列号50所示的互补决定区CDR3；

(C)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号52所示的互补决定区CDR1、序列号53所示的互补决定区CDR2、和序列号54所示的互补决定区CDR3，所述轻链可变区包含序列号56所示的互补决定区CDR1、序列号57所示的互补决定区CDR2、和序列号58所示的互补决定区CDR3；

(D)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号60所示的互补决定区CDR1、序列号61所示的互补决定区CDR2、和序列号62所示的互补决定区CDR3，所述轻链可变区包含序列号64所示的互补决定区CDR1、序列号65所示的互补决定区CDR2、和序列号66所示的互补决定区CDR3；

(E)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号170所示的互补决定区CDR1、序列号171所示的互补决定区CDR2、和序列号172所示的互补决定区CDR3，所述轻链可变区包含序列号173所示的互补决定区CDR1、序列号174所示的互补决定区CDR2、和序列号175所示的互补决定区CDR3；

(F)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号176所示的互补决定区CDR1、序列号177所示的互补决定区CDR2、和序列号178所示的互补决定区CDR3，所述轻链可变区包含序列号179所示的互补决定区CDR1、序列号180所示的互补决定区CDR2、和序列号181所示的互补决定区CDR3；

(G)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号182所示的互补决定区CDR1、序列号183所示的互补决定区CDR2、和序列号184所示的互补决定区CDR3，所述轻链可变区包含序列号185所示的互补决定区CDR1、序列号186所示的互补决定区CDR2、和序列号187所示的互补决定区CDR3；

(H)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号188所示的互补决定区CDR1、序列号189所示的互补决定区CDR2、和序列号190所示的互补决定区CDR3，所述轻链可变区包含序列号191所示的互补决定区CDR1、序列号192所示的互补决定区CDR2、和序列号193所示的互补决定区CDR3；

(I)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号146所示的互补决定区CDR1、序列号147所示的互补决定区CDR2、和序列号148所示的互补决定区CDR3，所述轻链可变区包含序列号149所示的互补决定区CDR1、序列号150所示的互补决定区CDR2、和序列号151所示的互补决定区CDR3；

(J)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号272所示的互补决定区CDR1、序列号273所示的互补决定区CDR2、和序列号274所示的互补决定区CDR3，所述轻链可变区包含序列号275所示的互补决定区CDR1、序列号276所示的互补决定区CDR2、和序列号277所示的互补决定区CDR3；

(K)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号290所示的互补决定区CDR1、序列号291所示的互补决定区CDR2、和序列号292所示的互补决定区CDR3，所述轻链可变区包含序列号293所示的互补决定区CDR1、序列号294所示的互补决定区CDR2、和序列号295所示的互补决定区CDR3；

(L)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号301所示的互补决定区CDR1、序列号302所示的互补决定区CDR2、和序列号303所示的互补决定区CDR3，所述轻链可变区包含序列号305所示的互补决定区CDR1、序列号306所示的互补决定区CDR2、和序列号307所示的互补决定区CDR3；

(M)包含重链可变区和轻链可变区、且与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段，所述重链可变区包含序列号134所示的互补决定区CDR1、序列号135所示的互补决定区CDR2、和序列号136所示的互补决定区CDR3，所述轻链可变区包含序列号137所示的互补决定区CDR1、序列号138所示的互补决定区CDR2、和序列号139所示的互补决定区CDR3。

8.根据权利要求1～7的任一项所述的药品，所述抗体或其片段是以下(a)～(al)的任一者，

(a)重链可变区包含序列号39的氨基酸序列、且轻链可变区包含序列号43的氨基酸序列的抗体或其片段；

(b)重链可变区包含序列号47的氨基酸序列、且轻链可变区包含序列号51的氨基酸序列的抗体或其片段；

(c)重链可变区包含序列号55的氨基酸序列、且轻链可变区包含序列号59的氨基酸序列的抗体或其片段；

(d)重链可变区包含序列号63的氨基酸序列、且轻链可变区包含序列号67的氨基酸序列的抗体或其片段；

(e)重链可变区包含序列号68的氨基酸序列、且轻链可变区包含序列号69的氨基酸序列的抗体或其片段；

(f)重链可变区包含序列号70的氨基酸序列、且轻链可变区包含序列号71的氨基酸序列的抗体或其片段；

(g)重链可变区包含序列号72的氨基酸序列、且轻链可变区包含序列号73的氨基酸序列的抗体或其片段；

(h)重链可变区包含序列号74的氨基酸序列、且轻链可变区包含序列号75的氨基酸序列的抗体或其片段；

(i)重链可变区包含序列号76的氨基酸序列、且轻链可变区包含序列号77的氨基酸序列的抗体或其片段；

(j)重链可变区包含序列号78的氨基酸序列、且轻链可变区包含序列号79的氨基酸序列的抗体或其片段；

(k)重链可变区包含序列号80的氨基酸序列、且轻链可变区包含序列号81的氨基酸序列的抗体或其片段；

(l)重链可变区包含序列号82的氨基酸序列、且轻链可变区包含序列号83的氨基酸序列的抗体或其片段；

(m)重链可变区包含序列号84的氨基酸序列、且轻链可变区包含序列号85的氨基酸序列的抗体或其片段；

(n)重链可变区包含序列号86的氨基酸序列、且轻链可变区包含序列号87的氨基酸序列的抗体或其片段；

(o)重链可变区包含序列号88的氨基酸序列、且轻链可变区包含序列号89的氨基酸序列的抗体或其片段；

(p)重链可变区包含序列号90的氨基酸序列、且轻链可变区包含序列号91的氨基酸序列的抗体或其片段；

(q)重链可变区包含序列号92的氨基酸序列、且轻链可变区包含序列号93的氨基酸序列的抗体或其片段；

(r)重链可变区包含序列号94的氨基酸序列、且轻链可变区包含序列号95的氨基酸序列的抗体或其片段；

(s)重链可变区包含序列号96的氨基酸序列、且轻链可变区包含序列号97的氨基酸序列的抗体或其片段；

(t)重链可变区包含序列号98的氨基酸序列、且轻链可变区包含序列号99的氨基酸序列的抗体或其片段；

(u)重链可变区包含序列号100的氨基酸序列、且轻链可变区包含序列号101的氨基酸序列的抗体或其片段；

(v)重链可变区包含序列号102的氨基酸序列、且轻链可变区包含序列号103的氨基酸序列的抗体或其片段；

(w)重链可变区包含序列号104的氨基酸序列、且轻链可变区包含序列号105的氨基酸序列的抗体或其片段；

(x)重链可变区包含序列号106的氨基酸序列、且轻链可变区包含序列号107的氨基酸序列的抗体或其片段；

(y)重链可变区包含序列号108的氨基酸序列、且轻链可变区包含序列号109的氨基酸序列的抗体或其片段；

(z)重链可变区包含序列号110的氨基酸序列、且轻链可变区包含序列号111的氨基酸序列的抗体或其片段；

(aa)重链可变区包含序列号112的氨基酸序列、且轻链可变区包含序列号113的氨基酸序列的抗体或其片段；

(ab)重链可变区包含序列号114的氨基酸序列、且轻链可变区包含序列号115的氨基酸序列的抗体或其片段；

(ac)重链可变区包含序列号116的氨基酸序列、且轻链可变区包含序列号117的氨基酸序列的抗体或其片段；

(ad)重链可变区包含序列号118的氨基酸序列、且轻链可变区包含序列号119的氨基酸序列的抗体或其片段；

(ae)重链可变区包含序列号120的氨基酸序列、且轻链可变区包含序列号121的氨基酸序列的抗体或其片段；

(af)重链可变区包含序列号122的氨基酸序列、且轻链可变区包含序列号123的氨基酸序列的抗体或其片段；

(ag)重链可变区包含序列号124的氨基酸序列、且轻链可变区包含序列号125的氨基酸序列的抗体或其片段；

(ah)重链可变区包含序列号126的氨基酸序列、且轻链可变区包含序列号127的氨基酸序列的抗体或其片段；

(ai)重链可变区包含序列号128的氨基酸序列、且轻链可变区包含序列号129的氨基酸序列的抗体或其片段；

(aj)重链可变区包含序列号130的氨基酸序列、且轻链可变区包含序列号131的氨基酸序列的抗体或其片段；

(ak)重链可变区包含序列号132的氨基酸序列、且轻链可变区包含序列号133的氨基酸序列的抗体或其片段；

(al)重链可变区包含序列号300的氨基酸序列、且轻链可变区包含序列号304的氨基酸序列的抗体或其片段。

9.根据权利要求1～8的任一项所述的药品，所述抗体是人抗体、人源化抗体、嵌合抗体或单链抗体。

10.根据权利要求1～9的任一项所述的药品，所述癌是在细胞膜表面表达CAPRIN-1蛋白质的癌。

11.根据权利要求1～10的任一项所述的药品，所述癌是卵巢癌、胆管癌、乳癌、肾癌、胰癌、大肠癌、黑素瘤、肺癌、肾细胞癌、霍奇金淋巴瘤、头颈癌、胃癌、间皮癌、结肠/直肠癌、食道癌、食管胃结合部癌、肝细胞癌、胶质母细胞瘤、尿路上皮癌、膀胱癌、子宫癌、原发性中枢神经系统淋巴瘤、原发性睾丸淋巴瘤、胆道癌、脑肿瘤、前列腺癌、白血病、淋巴瘤、肝癌、肉瘤、纤维肉瘤、肥大细胞瘤、肾上腺皮质癌、尤因肉瘤、多发性骨髓瘤、睾丸癌、甲状腺癌、基底细胞癌、佩吉特病或皮肤癌。

12.癌的治疗和/或预防用药物组合物的药效增强剂，所述癌的治疗和/或预防用药物组合物以与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段作为有效成分，所述药效增强剂以拓扑异构酶I抑制剂作为有效成分。

13.癌的治疗和/或预防用药物组合物的药效增强剂，所述癌的治疗和/或预防用药物组合物以拓扑异构酶I抑制剂作为有效成分，所述药效增强剂以与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段作为有效成分。

14.用于癌的治疗和/或预防的方法，其特征在于，将与CAPRIN-1蛋白质具有免疫反应性的抗体或其片段、和拓扑异构酶I抑制剂一起或各自分开地施与受检者。