CN117281833A - 丁香碳点在制备广谱抗菌药物中的应用 - Google Patents
丁香碳点在制备广谱抗菌药物中的应用 Download PDFInfo
- Publication number
- CN117281833A CN117281833A CN202311238066.XA CN202311238066A CN117281833A CN 117281833 A CN117281833 A CN 117281833A CN 202311238066 A CN202311238066 A CN 202311238066A CN 117281833 A CN117281833 A CN 117281833A
- Authority
- CN
- China
- Prior art keywords
- cds
- clove
- antibacterial
- carbon
- broad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000016639 Syzygium aromaticum Nutrition 0.000 title claims abstract description 26
- 244000223014 Syzygium aromaticum Species 0.000 title claims abstract description 26
- 229940124350 antibacterial drug Drugs 0.000 title claims abstract description 11
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims description 19
- 238000002360 preparation method Methods 0.000 title claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 33
- 241000588724 Escherichia coli Species 0.000 claims abstract description 25
- 241000191967 Staphylococcus aureus Species 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 241001104043 Syringa Species 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims 1
- 238000004729 solvothermal method Methods 0.000 claims 1
- 238000001308 synthesis method Methods 0.000 claims 1
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 26
- 230000001580 bacterial effect Effects 0.000 abstract description 26
- 210000004027 cell Anatomy 0.000 abstract description 13
- 231100000135 cytotoxicity Toxicity 0.000 abstract description 11
- 230000003013 cytotoxicity Effects 0.000 abstract description 11
- 230000001954 sterilising effect Effects 0.000 abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 10
- 210000002490 intestinal epithelial cell Anatomy 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 230000009471 action Effects 0.000 abstract description 4
- 230000006907 apoptotic process Effects 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 238000009792 diffusion process Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000001027 hydrothermal synthesis Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 210000005056 cell body Anatomy 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 10
- 239000007787 solid Substances 0.000 description 9
- 235000013599 spices Nutrition 0.000 description 7
- 239000003242 anti bacterial agent Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 230000005284 excitation Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 235000007650 Aralia spinosa Nutrition 0.000 description 3
- 238000002441 X-ray diffraction Methods 0.000 description 3
- 241000949456 Zanthoxylum Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 239000002086 nanomaterial Substances 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 2
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 235000013628 Lantana involucrata Nutrition 0.000 description 2
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 description 2
- 240000007673 Origanum vulgare Species 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000017803 cinnamon Nutrition 0.000 description 2
- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 238000009920 food preservation Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000000341 volatile oil Substances 0.000 description 2
- 240000006927 Foeniculum vulgare Species 0.000 description 1
- 235000004204 Foeniculum vulgare Nutrition 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000186779 Listeria monocytogenes Species 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 108010013639 Peptidoglycan Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
- 108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
- FZWLAAWBMGSTSO-UHFFFAOYSA-N Thiazole Chemical compound C1=CSC=N1 FZWLAAWBMGSTSO-UHFFFAOYSA-N 0.000 description 1
- 244000250129 Trigonella foenum graecum Species 0.000 description 1
- 235000001484 Trigonella foenum graecum Nutrition 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 238000000026 X-ray photoelectron spectrum Methods 0.000 description 1
- 244000089698 Zanthoxylum simulans Species 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000011852 carbon nanoparticle Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229910021389 graphene Inorganic materials 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 238000002173 high-resolution transmission electron microscopy Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002082 metal nanoparticle Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- AJDUTMFFZHIJEM-UHFFFAOYSA-N n-(9,10-dioxoanthracen-1-yl)-4-[4-[[4-[4-[(9,10-dioxoanthracen-1-yl)carbamoyl]phenyl]phenyl]diazenyl]phenyl]benzamide Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C=CC=C2NC(=O)C(C=C1)=CC=C1C(C=C1)=CC=C1N=NC(C=C1)=CC=C1C(C=C1)=CC=C1C(=O)NC1=CC=CC2=C1C(=O)C1=CC=CC=C1C2=O AJDUTMFFZHIJEM-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 235000001019 trigonella foenum-graecum Nutrition 0.000 description 1
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000001043 yellow dye Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/44—Elemental carbon, e.g. charcoal, carbon black
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/18—Nanoonions; Nanoscrolls; Nanohorns; Nanocones; Nanowalls
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Nanotechnology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Inorganic Chemistry (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Dentistry (AREA)
- Organic Chemistry (AREA)
- Epidemiology (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- General Physics & Mathematics (AREA)
- Materials Engineering (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Oncology (AREA)
- Composite Materials (AREA)
- Manufacturing & Machinery (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Abstract
本发明公开了一种丁香碳点在制备广谱抗菌药物中的应用，所述碳点是以丁香为原料，通过一步水热法合成，其尺寸较小，为2nm左右，可通过扩散作用进入细菌细胞体内，产生大量活性氧，导致细菌细胞凋亡。通过抗菌实验对比发现，丁香碳点对金黄色葡萄球菌和大肠杆菌的抗菌性能良好，对大肠杆菌的最小杀菌浓度为3mg/mL，对金黄色葡萄球菌的最小杀菌浓度为6mg/mL。同时，在MODE‑K小鼠小肠上皮细胞中加入120mg/mL抗菌药物培养24h后，细胞活力保持在90％以上，表明丁香碳点的细胞毒性较低，可广泛应用于抗菌领域。因此本发明为生物医学领域提供了一种新型的低细胞毒性抗菌药物。
Description
技术领域
本发明属于抗菌技术领域，具体涉及一种丁香碳点在制备广谱抗菌药物中的应用。
背景技术
食源性致病菌是指以食物为载体引起人类发生疾病的一类细菌微生物，主要有大肠杆菌、金黄色葡萄球菌、单核细胞增生李斯特氏菌、沙门氏菌、志贺氏菌等。据世界卫生组织估计，全球每年有6亿人患食源性疾病，死亡人数达42万，对人类健康构成重大威胁。尽管抗生素可以克服食源性致病菌的感染，但抗生素的过度使用和细菌的进化导致了抗生素耐药性菌株的出现，这进一步导致了细菌突变增加、疫苗短缺和伤口感染等问题。因此，开发不同于抗生素的高效抗菌剂是必要的。据报道，抗菌纳米颗粒包括碳基纳米材料、金属纳米颗粒、半导体纳米颗粒和聚合物纳米材料等。碳点作为一种典型的碳基纳米材料，易于制备且成本低廉，与传统抗生素相比，碳点具有较小的纳米尺寸、较低的细胞毒性，有较大的抗菌应用潜力。
香辛料具有香、辣、辛、麻等典型的气味，富含酚类化合物和精油，具有一定的抗癌、抗衰、抗炎等生物活性。香辛料提取物可用于抗菌和食品包装领域，以延长食品的保质期。但精油、酚类等提取过程繁琐，成品价格昂贵。与有机试剂合成的碳点相比，香辛料碳点细胞毒性更低。香辛料碳点是指将香辛料经过高温熬制之后，弃掉滤渣，保留所产生的香辛料炭质纳米颗粒，其制备过程简单，成本低廉。据报道，以葫芦巴、丁香、小茴香为原料合成碳点，利用其良好的荧光性能可测定食品样品中日落黄染料的含量。目前，香辛料碳点的抗菌效应的研究较少。有研究表明当花椒碳点的浓度分别为0.1％、0.25％、0.5％时，金黄色葡萄球菌的存活率为70％、65％、20％。虽然花椒碳点对金黄色葡萄球菌有一定的抑菌性能，但抑菌性能较差。
发明内容
本发明的目的是提供一种抗菌性能好且安全低毒的丁香碳点在制备广谱抗菌药物中的应用。
上述丁香碳点的制备方法为：将粉末状的丁香放入装有超纯水的聚四氟乙烯内衬的不锈钢高压釜中，在140～240℃保温3～7小时。
作为优选，上述丁香碳点的制备方法中，在220℃保温5小时。
作为优选，上述丁香碳点的制备方法中，粉末状的丁香与纯水的质量体积比为8～12g：100mL。
作为优选，上述丁香碳点在制备广谱抗菌药物中的应用，所述菌为大肠杆菌和金黄色葡萄球菌。
本发明的有益效果如下：
1.本发明的丁香碳点制备原料廉价易得，制备方法为一步水热法，制备方法简单，成本低，制备的丁香碳点具有生物相容性和低细胞毒性，其尺寸较小，为2nm左右，可通过扩散作用进入细菌细胞体内，产生大量活性氧，导致细菌细胞凋亡。
2.本发明丁香碳点对金黄色葡萄球菌和大肠杆菌的抗菌性能好，对大肠杆菌的最小杀菌浓度为3mg/mL；对金黄色葡萄球菌的最小杀菌浓度为6mg/mL。同时，在MODE-K小鼠小肠上皮细胞中加入120mg/mL抗菌药物培养24h后，细胞活力保持在90％以上，表明丁香碳点的细胞毒性较低，可广泛应用于生物医学和食品保鲜及包装的抗菌领域。
附图说明
图1是不同碳点的透射电镜以及高分辨率透射电镜图(A)和粒径分布图(B)。
图2是不同碳点的X射线衍射图谱。
图3是不同碳点的傅里叶红外光谱图。
图4是不同碳点的X射线光电子能谱图。
图5是不同碳点的Zeta电位图。
图6是不同碳点的紫外可见吸收光谱以荧光发射光谱图。
图7是不同碳点对金黄色葡萄球菌和大肠杆菌抗菌性能的对比。
图8是不同浓度的丁香碳点对大肠杆菌及金黄色葡萄球菌杀菌效果的影响。
图9是大肠杆菌在丁香碳点作用前(A)后(B)的扫描电镜图片。
图10是金黄色葡萄球菌在丁香碳点作用前(A)后(B)的扫描电镜图片。
图11是丁香碳点对大肠杆菌核酸(A)和蛋白质(B)泄漏量的影响。
图12是丁香碳点对金黄色葡萄球菌核酸(A)和蛋白质(B)泄漏量的影响。
图13是MODE-K小鼠小肠上皮细胞在不同浓度的丁香碳点下的存活率。
具体实施方式
下面结合附图和实施例对本发明进一步详细说明，但本发明的保护范围不仅限于这些实施例。
实施例1
称取2g粉末状的丁香并放入装有20mL超纯水的聚四氟乙烯内衬的不锈钢高压反应釜中，将反应釜升温至220℃后持续加热5小时，待反应釜自然冷却至室温后，将所得溶液装至离心管，以6500rpm离心处理15min，上清液通过0.22μm滤膜除去大颗粒不溶物，所得滤液通过透析膜(MWCO＝3500Da)进行纯化，每隔2小时换一次水，除去未反应的物质，经冷冻干燥获得丁香碳点(记为Cl-CDs)，4℃下储存。
分别以粉末状的桂皮、花椒、牛至为原料，按照实施例1的方法制备桂皮碳点(Ci-CDs)、花椒碳点(Si-CDs)、牛至碳点(Or-CDs)，作为对比样，进行对比试验。
如图1A所示，Ci-CDs、Cl-CDs、Or-CDs均呈球形状，分散性良好，无堆积或聚集现象。图1A插图为Ci-CDs、Cl-CDs、Or-CDs的HR-TEM图像，从中观察到衬度明暗变化，测量得它们的晶格间距依次为0.50nm、0.30nm、0.50nm，这与石墨烯(002)的晶格间距非常接近。从图1B中观察到Ci-CDs、Cl-CDs、Or-CDs的粒径分布范围大致为1.2～3nm，三者的平均粒径依次为1.86nm、1.55nm、2.96nm。Ci-CDs、Cl-CDs、Or-CDs的XRD谱图如图2所示，它们分别在2θ为20.22°、22.63°、20.53°显示出较宽的谱带，该谱带与石墨碳的晶面间距有关，与此同时表明以上三种碳点均具有无定形结构。对Ci-CDs、Cl-CDs、Or-CDs的表面化学基团进行了FT-IR表征，如图3所示，3387cm-1处的强吸收峰归因于O-H伸缩振动，2840cm-1处的小吸收带是由C-H键的伸缩振动而导致的，位于1600cm-1和1072cm-1处的吸收峰分别代表C＝C和C-O键的伸缩振动，这表明Ci-CDs、Cl-CDs、Or-CDs表面存在羟基(-OH)和羧基(-COOH)，因此以上3种碳点在水中的分散性良好。1285cm-1处的振动峰由C-O-C的弯曲振动造成，代表C-N键(1401cm-1)的伸缩振动的吸收峰表明碳点表面有氮元素的掺杂。图4的XPS图像进一步分析了Ci-CDs、Cl-CDs、Or-CDs的化学构成，三者的总谱图展现出三个明显的元素特征峰，分别为C1s(285eV)、N1s(400eV)、C1s(532eV)，从三者的C1s谱可知，C＝C/C-C的结合能为284.8eV，Ci-CDs的C1s高分辨光谱还可观察到两个峰，分别代表C-O/C-N(286.41eV)、C＝O/C＝N(288.37eV)，Cl-CDs和Or-CDs的C1s光谱图与Ci-CDs十分相似，Cl-CDs的C-O/C-N和C＝O/C＝N的结合能分别为286.32eV和288.32eV，Or-CDs的C-O/C-N和C＝O/C＝N的结合能分别为286.28eV和288.26eV。XPS图谱与FT-IR图谱均表明Ci-CDs、Cl-CDs、Or-CDs三者的化学构成相似，碳点表面都可能保留了大量的羟基和羧基。Ci-CDs、Cl-CDs、Or-CDs的Zeta电位测试如图5所示，三者均为负电荷碳点，Zeta电位依次为-13.3、-18.5和-19.5mV。Ci-CDs、Cl-CDs、Or-CDs在225～550nm范围内的紫外可见光谱及荧光发射光谱如图6所示，三者的紫外光谱图均在275nm左右处有一个特征吸收峰，这源于碳点表面C-C键上的π-π*电子跃迁，从图中可以看出Ci-CDs、Cl-CDs、Or-CDs三者的荧光性能良好，但最佳激发波长和荧光发射波长有所迥异，Ci-CDs在356nm的激发下具有强烈的荧光发射(440nm)，Cl-CDs在345nm的激发下具有强烈的荧光发射(440nm)，Or-CDs在368nm的激发下具有强烈的荧光发射(460nm)。
对上述Ci-CDs、Cl-CDs、Or-CDs、Si-CDs的抗菌性能进行了对比测试，具体试验情况如下：
1、抗菌性能试验
挑取保存至4℃冰箱的平板上的单个菌落(大肠杆菌和金黄色葡萄球菌)，并置于25mL液体培养基中，在37℃摇床中孵育15h。之后用移液枪吸取浑浊菌液于离心管中，以6500rpm的转速处理2min后，在离心管底部观察到明显的菌液沉淀，收集后用0.01MPBS缓冲液重复洗涤3次，以除去液体培养基。最后用0.01M PBS缓冲液将菌液沉淀重悬至1mL，梯度稀释至106倍备用。
取4℃下储存的Ci-CDs、Cl-CDs、Or-CDs、Si-CDs的原液各100μL，再加入100μL大肠杆菌菌液作为实验组，将100μL0.01MPBS缓冲液与100μL大肠杆菌菌液的混合液作为空白组，37℃震荡孵育2h，之后吸取100μL涂于LB固体培养基上，37℃培养15h，并记录菌落的生长情况。Ci-CDs、Cl-CDs、Or-CDs、Si-CDs对金黄色葡萄球菌的抗菌性能实验同上，只需将100μL大肠杆菌菌液、LB固体培养基和孵育2h替换为100μL金黄色葡萄球菌菌液、BHI固体培养基和孵育3h即可。
采用活菌计数法对Ci-CDs、Cl-CDs、Or-CDs、Si-CDs的抗菌性能进行比较。杀菌率计算公式为：杀菌率％＝(M0-M)/M0×100％，其中M0为空白组的菌落数量，M为实验组的菌落数量。
从图7中可以看出，Ci-CDs、Cl-CDs、Or-CDs、Si-CDs对大肠杆菌和金黄色葡萄球菌均存在一定的抑菌性。对大肠杆菌和金黄色葡萄球菌抗菌性能从大到小依次为Cl-CDs<Or-CDs<Ci-CDs<Si-CDs。即Cl-CDs具有更优越的抗菌性能，以下操作均以Cl-CDs为代表进行探究。
进一步以大肠杆菌和金黄色葡萄球菌为模型，采用平板计数法对Cl-CDs的最小杀菌浓度进行探究。具体方法为：用0.01M PBS缓冲液将Cl-CDs的浓度分别调整为1mg/mL、2mg/mL、3mg/mL、6mg/mL、12mg/mL，分别吸取不同浓度的Cl-CDs溶液100μL(实验组)和0.01MPBS缓冲液(空白组)，与100μL大肠杆菌菌液混合孵育2h，之后吸取100μL涂于LB固体培养基上，37℃培养15h，并记录菌落的生长情况。Cl-CDs对金黄色葡萄球菌的最小杀菌浓度测定同大肠杆菌，只需将100μL大肠杆菌菌液、LB固体培养基以及孵育时间2h替换为100μL金黄色葡萄球菌菌液、BHI固体培养基以及孵育时间3h即可。
如图8所示，随着Cl-CD浓度的逐渐增大，抗菌性能呈上升趋势。当Cl-CD的浓度为3mg/mL时，固体琼脂板上几乎观察不到大肠杆菌菌落，杀菌率高达99％；而固体琼脂板上呈现出明显的金黄色葡萄球菌菌落，杀菌率为45％。而当Cl-CD的浓度为6mg/mL时，固体琼脂板上几乎观察不到金黄色葡萄球菌菌落，杀菌率高达99％。Cl-CDs对大肠杆菌和金黄色葡萄球菌的最小杀菌浓度之所以不同，可能与二者的肽聚糖层的厚度有关，相较于大肠杆菌而言，金黄色葡萄球菌的肽聚糖层厚且致密，抑制了Cl-CDs进入金黄色葡萄球菌细胞内的速度和效率。
通过SEM观测了未经Cl-CDs处理和经过Cl-CDs处理的细菌细胞形态。图9A和图10A是未经Cl-CDs处理的大肠杆菌和金黄色葡萄球菌，从图中可以清楚地观察到细胞壁结构完整且表面光滑。图9B和图10B是经过Cl-CDs处理的大肠杆菌和金黄色葡萄球菌，图中的细菌细胞壁褶皱且塌陷，表明细胞壁明显受损，且细胞内容物流出，最终导致细胞凋亡，这是由于Cl-CDs刺激细菌细胞产生ROS所致。
260nm和280nm处吸光值可以直观反映细菌细胞体内泄漏到体外的DNA和蛋白质浓度变化，一定程度上也反映了细胞膜的完整性。如图11A和12A所示，同浓度下菌液和Cl-CDs的OD260均小于菌液和Cl-CDs混合液；从图11B和12B中也可以看出同浓度下菌液和Cl-CDs的OD280均小于菌液和Cl-CDs混合液。OD260和OD280的变化与Cl-CDs的抗菌性能有关。经Cl-CDs刺激后OD260和OD280明显上升，表明细菌细胞膜被破环，导致体内的DNA和蛋白质溢出，因此吸光值增加。
2、细胞毒性测试
Cl-CDs的低毒性是在生物医学和食品防腐等领域应用的关键因素。以MODE-K小鼠小肠上皮细胞为模型，采用噻唑蓝(MTT)法对Cl-CDs的细胞毒性进行了探究。MTT法测试原理为：活细胞中琥珀酸脱氢酶将MTT还原为甲臜(不溶于水的结晶，也可被特定溶剂溶解)，而死细胞无法将MTT还原，因此，OD570与Cl-CDs的细胞毒性成正比。具体方法为：在96孔板中加入100μL MODE-K小鼠小肠上皮细胞(5000个/孔)，待4～5h后细胞贴壁，分别加入0mg/mL(空白组)、3mg/mL、6mg/mL、30mg/mL、60mg/mL、120mg/mL Cl-CDs溶液(实验组)，将96孔板置于37℃通有5％CO2的培养箱中24h，之后每孔中加入10μL5mg/mL的MTT，混匀后置于培养箱中4h，将每孔中的培养基小心吸出，并加入100μL甲臜溶解液，轻轻振荡混匀，测其570nm处的吸光值。细胞毒性计算公式为：细胞毒性(％)＝A/A0×100％，A为实验组在570nm处的吸光值；A0为空白组在570nm处的吸光值。
如图13所示，用3mg/mL、6mg/mL、30mg/mL、60mg/mL、120mg/mL五种不同浓度的Cl-CDs处理MODE-K小鼠小肠上皮细胞，细胞存活率分别为102％、101％、98％、97％、90％。即使Cl-CDs浓度较大时，细胞存活率仍然较高。表明Cl-CDs的细胞毒性较低，可广泛应用于抗菌领域。
Claims (4)
1.丁香碳点在制备广谱抗菌药物中的应用，其特征在于，所述丁香碳点是以丁香为原料，通过溶剂热法合成，具体合成方法为：将粉末状的丁香放入装有超纯水的聚四氟乙烯内衬的不锈钢高压釜中，在140～240℃保温3～7小时。
2.根据权利要求1所述的丁香碳点在制备广谱抗菌药物中的应用，其特征在于，在220℃保温5小时。
3.根据权利要求1所述的丁香碳点在制备广谱抗菌药物中的应用，其特征在于，所述粉末状的丁香与纯水的质量体积比为8～12g：100mL。
4.根据权利要求1所述的丁香碳点在制备广谱抗菌药物中的应用，其特征在于，所述菌为大肠杆菌和金黄色葡萄球菌。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311238066.XA CN117281833A (zh) | 2023-09-25 | 2023-09-25 | 丁香碳点在制备广谱抗菌药物中的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311238066.XA CN117281833A (zh) | 2023-09-25 | 2023-09-25 | 丁香碳点在制备广谱抗菌药物中的应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117281833A true CN117281833A (zh) | 2023-12-26 |
Family
ID=89253046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311238066.XA Pending CN117281833A (zh) | 2023-09-25 | 2023-09-25 | 丁香碳点在制备广谱抗菌药物中的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117281833A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669680A (zh) * | 2022-09-28 | 2023-02-03 | 广东石油化工学院 | 一种碳量子点及其在防治植物病害中的应用 |
-
2023
- 2023-09-25 CN CN202311238066.XA patent/CN117281833A/zh active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669680A (zh) * | 2022-09-28 | 2023-02-03 | 广东石油化工学院 | 一种碳量子点及其在防治植物病害中的应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Selim et al. | Facile design of reduced graphene oxide decorated with Cu2O nanocube composite as antibiofilm active material | |
| Rahmati et al. | Ag-doped magnetic metal organic framework as a novel nanostructured material for highly efficient antibacterial activity | |
| Nagvenkar et al. | A one-step sonochemical synthesis of stable ZnO–PVA nanocolloid as a potential biocidal agent | |
| CN117281833A (zh) | 丁香碳点在制备广谱抗菌药物中的应用 | |
| CN111248224A (zh) | 基于MXene量子点的抗菌剂的制备及抗菌活性测试方法 | |
| CN110074136A (zh) | 一种铜铁氧化物及混合纳米颗粒的制备方法和抗菌应用 | |
| Mohammed et al. | Assessment of antimicrobial activity of chitosan/silver nanoparticles hydrogel and cryogel microspheres | |
| Wrońska et al. | Antimicrobial effect of chitosan films on food spoilage bacteria | |
| CN112662636A (zh) | 一种沙门氏菌广谱烈性噬菌体、制备方法及其应用 | |
| Nikparast et al. | Synergistic effect between phyto-syntesized silver nanoparticles and ciprofloxacin antibiotic on some pathogenic bacterial strains | |
| CN111066792A (zh) | 一种抗菌剂组合物及应用 | |
| CN112159798B (zh) | 一株针对高毒力肺炎克雷伯氏菌的新噬菌体及其应用 | |
| CN113403069A (zh) | 碳量子点、基于碳量子点含纳米银的高效抗菌材料及其制法 | |
| Purohit et al. | Chitosan-based multifunctional films reinforced with cerium oxide nanoparticles for food packaging applications | |
| Harandi et al. | Surface modification of electrospun wound dressing material by Fe2O3 nanoparticles incorporating Lactobacillus strains for enhanced antimicrobial and antibiofilm activity | |
| Hamza et al. | Evaluation the effect of green synthesis titanium dioxide nanoparticles on Acinetobacter baumannii isolates. | |
| M'barek et al. | Nanocellulose synthesis from Tamarix aphylla and preparation of hybrid nanocellulose composites membranes with investigation of antioxidant and antibacterial effects | |
| CN114569558A (zh) | 辣木秸秆介导合成生物碲纳米颗粒及其抗菌抗病毒应用 | |
| Shehab et al. | Virus and bacterial removal ability of TiO2 nanowire-based self-supported hybrid membranes | |
| CN113025318B (zh) | 一种以花椒为碳源的碳量子点及其制备方法与应用 | |
| Jamil et al. | Encapsulation of cardamom essential oil in chitosan nano-composites: in-vitro efficacy on antibiotic-resistant bacterial pathogens and cytotoxicity studies. Front. Microbiol. 7, 1580 | |
| Alayande et al. | Antibacterial rGO–CuO–Ag film with contact-and release-based inactivation properties | |
| Dai et al. | Effect of zinc oxide film morphologies on the formation of Shewanella putrefaciens biofilm | |
| Selvakumar et al. | Antibacterial efficacy of Phytosynthesized multi-metal oxide nanoparticles against drug-resistant foodborne pathogens | |
| CN114469893B (zh) | 一种季铵盐化二氧化硅纳米颗粒及制备方法与应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |