CN117185878A - Plant nutrient solution and application thereof in dragon fruit cultivation and preparation method thereof - Google Patents
Plant nutrient solution and application thereof in dragon fruit cultivation and preparation method thereof Download PDFInfo
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 73
- 241000196324 Embryophyta Species 0.000 title claims abstract description 48
- 244000157072 Hylocereus undatus Species 0.000 title claims abstract description 44
- 235000018481 Hylocereus undatus Nutrition 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 238000012364 cultivation method Methods 0.000 title description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 166
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 90
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 210000004209 hair Anatomy 0.000 abstract description 26
- 239000004471 Glycine Substances 0.000 abstract description 10
- 239000002028 Biomass Substances 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 24
- 240000008086 Echinocereus enneacanthus Species 0.000 description 15
- 235000010837 Echinocereus enneacanthus subsp brevispinus Nutrition 0.000 description 15
- 235000006850 Echinocereus enneacanthus var dubius Nutrition 0.000 description 15
- 230000012010 growth Effects 0.000 description 14
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 10
- 238000011282 treatment Methods 0.000 description 10
- 229910002651 NO3 Inorganic materials 0.000 description 9
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 9
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 210000000805 cytoplasm Anatomy 0.000 description 4
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- 235000013922 glutamic acid Nutrition 0.000 description 4
- 239000004220 glutamic acid Substances 0.000 description 4
- 239000003642 reactive oxygen metabolite Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
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- 206010038933 Retinopathy of prematurity Diseases 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000219357 Cactaceae Species 0.000 description 1
- 235000001808 Ceanothus spinosus Nutrition 0.000 description 1
- 241001264786 Ceanothus spinosus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
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- IZEKFCXSFNUWAM-UHFFFAOYSA-N dipyridamole Chemical compound C=12N=C(N(CCO)CCO)N=C(N3CCCCC3)C2=NC(N(CCO)CCO)=NC=1N1CCCCC1 IZEKFCXSFNUWAM-UHFFFAOYSA-N 0.000 description 1
- 229960002768 dipyridamole Drugs 0.000 description 1
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- HNONEKILPDHFOL-UHFFFAOYSA-M tolonium chloride Chemical compound [Cl-].C1=C(C)C(N)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 HNONEKILPDHFOL-UHFFFAOYSA-M 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The application relates to the technical field of planting and cultivation, in particular to a plant nutrient solution and application thereof in dragon fruit cultivation and a preparation method thereof. A plant nutrient solution comprises a basic nutrient solution, a first nitrogen source and a second nitrogen source, wherein the basic nutrient solution is Hoagland nutrient solution diluted by one time by adding water, and the first nitrogen source comprises NH 4 + -N and NO 3 And the second nitrogen source at least comprises glycine-N, and the molar concentration of the first nitrogen source and the second nitrogen source in the plant nutrient solution is 1.5-4 mM. The preparation method is also provided, and a first nitrogen source and a second nitrogen source are added into the Hoagland nutrient solution which is diluted by 1 time by adding water. The glycine-containing plant nutrient solution provided by the embodiment of the application shortens the length of the root system and increases the root hair length of the root system of the dragon fruit, thereby affecting the nutrition and the quality of the plant nutrient solutionThe absorption of moisture, the number of lateral branches, the length of the lateral branches and the biomass of the stems of the dragon fruit seedlings are obviously improved.
Description
Technical Field
The application relates to the technical field of planting and cultivating of dragon fruits, in particular to a plant nutrient solution and application thereof in cultivating of dragon fruits and a preparation method thereof.
Background
The dragon fruit is a perennial climbing plant of the genus dipyridamole of the family Cactaceae, is also an emerging nutritional fruit, and has high economic value. Dragon fruits originate in Central America and are currently mainly distributed in China in Hainan, guangdong, guangxi, fujian and other places. The yield of the dragon fruits is high, the average yield per hectare is 50-150 tons, and the dragon fruit planting has become an important economic support of agricultural industry structures in tropical and subtropical areas.
Nitrogen is one of the main nutritional elements required by the dragon fruit, and nitrogen application is beneficial to the growth of the root system and the branches of the dragon fruit, so that high yield is maintained. The requirement of the dragon fruits on nitrogen is large in commercial production, and accurate management of nitrogen is a key technology in the planting of the dragon fruits. Nitrogen morphology and nitrogen application amount influence on dragon fruit clean CO 2 The absorption capacity and the aboveground nitrogen content, as well as plant growth, fruit yield and quality, but it is not clear whether the nitrogen morphology affects the growth of pitaya root hairs at present.
The root hair is a tubular structural organ with a sealed top and protruding outwards, which is formed by differentiating plant root epidermal cells, is an important component of plant root systems, and plays an important role in absorbing moisture and nutrients from soil. The root hair is an important component of plant root system, and is easy to be clung to soil particles due to the thin and soft cell wall and adhesion, thus playing a role in fixation in plant growth. Root hairs can greatly increase the absorption area of roots, and elongated root hairs play an important role in cell growth and in absorbing moisture and nutrients which are transported to the endothelial cells of the roots and then to the aerial parts. Furthermore, root hairs can also improve stress resistance of plants through self adaptive reaction and root secretions thereof. The majority of the pitaya nutrient solution prepared in the prior art has a promoting effect on the root system length of the pitaya, but less research on the growth influence of the root hairs of the pitaya is carried out, and experiments show that the longer the root system of the pitaya is, the better the yield and the quality of the pitaya are not necessarily, so in order to improve the quality and the efficiency of the field root-promoting cultivation technology, the application needs to be provided with a plant nutrient solution capable of promoting the growth of the root hairs of the pitaya.
Disclosure of Invention
The application provides a plant nutrient solution, a preparation method and application thereof, and aims to solve the problems that most of the pitaya nutrient solution prepared by the prior technical means in the background art has a promotion effect on the root system length of the pitaya, but less research on the growth influence of root hairs of the pitaya is carried out, and experiments show that the longer the root system of the pitaya is, the better the yield and quality of the pitaya are not necessarily.
In order to solve the technical problems, the application adopts the following technical scheme: a plant nutrient solution comprises a basic nutrient solution, a first nitrogen source and a second nitrogen source, wherein the basic nutrient solution is Hoagland nutrient solution diluted by one time by adding water, and the first nitrogen source comprises NH 4 + -N and NO 3 And the second nitrogen source at least comprises glycine-N, and the molar concentration of the first nitrogen source and the second nitrogen source in the plant nutrient solution is 1.5-4 mM.
As a preferred embodiment, the second nitrogen source further comprises glutamate-N, wherein the molar concentration of glutamate-N is 2-3mM.
Preferably, the base nutrient solution comprises the following components in molar concentration:
0.5mM KH 2 PO 4 、1.25mM K 2 SO 4 、2.5mM CaCl 2 、1mM MgSO 4 ·7H 2 O、0.5mM EDTA-Fe、0.05mM FeSO 4 ·7H 2 O、0.05mM H 3 BO 3 、0.01mM MnSO 4 ·H 2 O、0.0008mM ZnSO 4 ·7H 2 O、0.0002mM CuSO 4 ·5H 2 O、0.0021mM Na 2 MoO 4 ·2H 2 O、0.0048mM KI。
as a preferred embodiment, the second nitrogen source is only glycine-N.
Preferably, the NH 4 +-N、NO 3 The molar concentration of both the N and the glycine-N is 2-3mM.
Preferably, the NH 4 +-N、NO 3 The molar concentrations of both-N and glycine-N were 2.5mM.
Preferably, the NH 4 + -N、NO 3 - N, glycine-N is derived from (NH) 4 ) 2 SO 4 、KNO 3 And L-glycine.
The plant nutrient solution in the technical scheme is mainly used for cultivating and planting the dragon fruits, as nitrogen with different forms is added into the Hoagland nutrient solution, the first nitrogen source is basic nitrogen sources such as ammonium nitrogen, nitrate nitrogen and the like commonly used in plant cultivation, nitrogen application is beneficial to growth of root systems and branches of the dragon fruits, and NH in cytoplasm 4+ The balance maintains root hair tip growth, and the additional addition of a second ammonia source, glycine, promotes root hair pair growth by regulating Reactive Oxygen Species (ROS) via ROPs to maintain a calcium ion concentration gradient in the cytoplasm, which is important for root hair tip growth.
It can be appreciated that the application of the plant nutrient solution in the cultivation of dragon fruits is also within the protection scope of the patent. The dragon fruit can be red heart dragon fruit, white heart dragon fruit and the like.
In addition, the preparation method of the plant nutrient solution is also provided, a first nitrogen source and a second nitrogen source are added into the Hoagland nutrient solution which is diluted by 1 time by adding water, and the first nitrogen source comprises NH 4 + -N and NO 3 -N, said second nitrogen source comprising at least glycine-N.
Preferably, the NH 4 + -N、NO 3 - N, glycine-N are each represented by (NH) 4 ) 2 SO 4 、KNO 3 And L-glycine formsAdded according to the formula.
Compared with the prior art, the beneficial effects are that: the glycine-containing plant nutrient solution provided by the application is used for cultivating the dragon fruit plants, obviously shortens the length of the root system of the dragon fruit, and increases the root hair length of the root system of the dragon fruit, thereby affecting the absorption of nutrients and moisture and obviously improving the side branch number, the side branch length and the stem biomass of the dragon fruit seedlings. The root system is longer and not necessarily better when the dragon fruit is cultivated, and on the premise of paying attention to root cultivation, the application improves the attention to root hair length, obviously improves the field root promotion cultivation technology, improves the growth performance of the dragon fruit plant, obviously improves the later fruiting yield and quality, and simultaneously further researches the influence of different nitrogen forms on plant root hair.
Drawings
FIG. 1 is a graph showing the effect of root hair length microstructure of examples 1-3 and comparative examples 1-3.
FIG. 2 is a graph showing the effect of the root length microstructure of examples 1-3 and comparative examples 1-3.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the present patent; for the purpose of better illustrating the embodiments, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the actual product dimensions; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationship depicted in the drawings is for illustrative purposes only and is not to be construed as limiting the present patent.
The same or similar reference numbers in the drawings of embodiments of the application correspond to the same or similar components; in the description of the present application, it should be understood that, if there are orientations or positional relationships indicated by terms "upper", "lower", "left", "right", "long", "short", etc., based on the orientations or positional relationships shown in the drawings, this is merely for convenience in describing the present application and simplifying the description, and is not an indication or suggestion that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, so that the terms describing the positional relationships in the drawings are merely for exemplary illustration and are not to be construed as limitations of the present patent, and that it is possible for those of ordinary skill in the art to understand the specific meaning of the terms described above according to specific circumstances. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. The "vertical" is not strictly vertical but is within the allowable error range. "parallel" is not strictly parallel but is within the tolerance of the error.
In the description of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and for example, the particular relationship represented may be either fixedly connected, detachably connected, or integrally connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. It will be understood by those of ordinary skill in the art that the specific meaning of the terms described above in this disclosure may be understood as appropriate.
The technical scheme of the application is further specifically described by the following specific embodiments with reference to the accompanying drawings:
in a first aspect, the application provides a plant nutrient solution mainly used for cultivation and planting of dragon fruits, which comprises the following components in molar concentration:
0.5mM KH 2 PO 4 、1.25mM K 2 SO 4 、2.5mM CaCl 2 、1mM MgSO 4 ·7H 2 O、0.5mM EDTA-Fe、0.05mM FeSO 4 ·7H 2 O、0.05mM H 3 BO 3 、0.01mM MnSO 4 ·H 2 O、0.0008mM ZnSO 4 ·7H 2 O、0.0002mM CuSO 4 ·5H 2 O、0.0021mM Na 2 MoO 4 ·2H 2 O、0.0048mM KI。
and a first nitrogen source and a second nitrogen source, the first nitrogen source including NH 4 + -N and NO 3 -N, the second nitrogen source being glycine-N.
In other embodiments, the second nitrogen source may further include other types of nitrogen sources other than glycine-N, for example, the second nitrogen source may be added with an appropriate molar concentration of an amino acid nitrogen source such as glutamate-N (glutamic acid), and the first nitrogen source may be added with an appropriate molar concentration of a conventional nitrogen source such as calcium nitrate, urea, and the like.
In a second aspect, the present application provides a method for preparing the plant nutrient solution, wherein a first nitrogen source and a second nitrogen source are added into the Hoagland nutrient solution diluted 1 time by water, the first nitrogen source comprises NH 4 + -N and NO 3 -N, the second nitrogen source being glycine-N. The specific method steps and the mode of application thereof in dragon fruit cultivation are as follows:
experimental materials: the dragon fruit plant is a Hainan Trinity dragon fruit base.
Plant growth conditions: the light and heat of the base are natural, the local sunshine hours are 2200 h, and the average annual air temperature is 24.5 ℃. The dragon fruits are planted in a branch cutting mode, and the weight of the branches to be tested is controlled at 110+/-5 g. The experiment adopts the method that the Hoagland plant nutrient solution diluted by 1 time by adding water is added with nitrogen in different forms, and the Hoagland plant nutrient solution diluted by 1 time by adding water comprises the following components in molar concentration: 0.5mM KH 2 PO 4 、1.25mM K 2 SO 4 、2.5mM CaCl 2 、1mM MgSO 4 ·7H 2 O、0.5mM EDTA-Fe、0.05mM FeSO 4 ·7H 2 O、0.05mM H 3 BO 3 、0.01mM MnSO 4 ·H 2 O、0.0008mM ZnSO 4 ·7H 2 O、0.0002mM CuSO 4 ·5H 2 O、0.0021mM Na 2 MoO 4 ·2H 2 O、0.0048mM KI。
Experimental treatment: NH (NH) 4 + -N、NO 3 - N, glycine-N is a catalyst selected from the group consisting of (NH) 4 ) 2 SO 4 、KNO 3 And L-glycine. Cleaning the dragon fruit mother plant before field planting, field planting in a PVC water planting tank with the capacity of 7.4 and L, intermittently ventilating the water planting tank by an electromagnetic air pump for 8:00-18:00 a day, ventilating for 20 min per hour, and replacing the water planting nutrient solution once in 7 days.
Sample collection and measurement: the overground part and the underground part are separated during sampling, the quantity of the lateral branches growing on the parent branch is recorded, and the total length of the lateral branches is measured. The impurities on the surface of the plants are lightly washed by tap water, then washed by deionized water for 3 times, then dried slightly, and the samples are sheared and respectively put into kraft paper envelopes marked in advance and put into an oven. 105. Deactivating enzyme for 30 min at deg.C, regulating temperature to 80deg.C, baking to constant weight, and weighing the dry weight of branch.
The root system is washed by deionized water, placed in 50% alcohol solution, and then placed in a refrigerator at 4 ℃ for preservation, so as to prevent the root system from deforming. A portion of each root representing approximately 1. 1 cm of the root hair length was cut out and stained with a toluidine blue solution at a concentration of 0.005%. As can be clearly seen from fig. 1 and 2, root length of pitaya seedlings cultivated with the plant nutrient solution of the present application is significantly reduced and root length is increased by photographing and measuring root length with Motic Images Plus 3.0.3.0 (x 64) software under a stereoscopic microscope.
For the plant nutrient solution in the application, an example and a comparative example are arranged, wherein the nutrient solution of the comparative example is treated by adding Amm +Nit (ammonium nitrogen+nitrate nitrogen) into 1/2 Hoagland nutrient solution, namely, only a first nitrogen source is added; the example adopts the plant nutrient solution in the application, namely, amm +Nit+Gly (ammonium nitrogen+nitrate nitrogen+glycine) is added into 1/2 Hoagland nutrient solution for treatment, and then the side branch number, the side branch length and the stem biomass of the dragon fruit seedlings under different nutrient solution treatments are measured, so that the following data are obtained:
TABLE 1 determination of the number of side branches, the length of the side branches and the stem biomass of seedlings of Pitaya treated with different nitrogen types
| Treatment group | Number of side branches | Length of side branch (cm) | Stem biomass |
| Comparative example | 3.0±0.7 | 35.6±5.7 | 9.45±2.08 |
| Examples | 5.8±0.6 | 61.9±6.0 | 18.12±1.76 |
In addition, for the measurement and comparison of root hairs and root systems, a plurality of examples and comparative examples were also tested,
examples 1-8 were prepared by adding Amm +nit+gly (ammonium nitrogen+nitrate nitrogen+glycine) three nitrogen sources to the plant nutrient solution of the application, namely, the double diluted Hoagland nutrient solution, to cultivate the dragon fruit seedlings.
Wherein, in examples 1-3, the molar concentration of each nitrogen source was 2.5mM;
in example 4, the molar concentration of each nitrogen source was 1.5mM;
in example 5, the molar concentration of each nitrogen source was 4mM;
in example 6, the molar concentration of each nitrogen source was 3mM;
in example 7, glutamic acid was further added at a molar concentration of 2mM based on example 1;
in example 8, glutamic acid was further added at a molar concentration of 3mM based on example 1;
the nutrient solutions of comparative examples 1-3 were treated with the Hoagland nutrient solution diluted one-time with water by adding Amm +Nit (ammonium nitrogen+nitrate nitrogen) alone, each nitrogen source having a molar concentration of 2.5mM; comparative example 4. Hoagland's nutrient solution diluted by one time with water was added with Amm +Nit+Glu (ammonium nitrogen+nitrate nitrogen+glutamic acid) three nitrogen sources, each of which has a molar concentration of 2.5mM, to cultivate dragon fruit seedlings; comparative examples 5-6 the seedlings of dragon fruits were cultivated with a conventional nutrient solution (exquisite flower); comparative example 7 the young dragon seedlings were cultivated by adding Amm +nit+gly (ammonium nitrogen+nitrate nitrogen+glycine) three nitrogen sources, each of which has a molar concentration of 5mM, to the holland nutrient solution diluted one time with water, and comparative example 8 the young dragon seedlings were cultivated by adding Amm +nit+gly (ammonium nitrogen+nitrate nitrogen+glycine) three nitrogen sources, each of which has a molar concentration of 1mM.
The average root lengths and average root lengths of comparative examples 1-8 and examples 1-8 were then measured, respectively, to obtain the following data:
TABLE 2 root length and root length of Pitaya seedlings treated with different Nitrogen types
| Treatment group | Root length (mum) | Root length (cm) |
| Example 1 | 3079.39 | 6.5 |
| Example 2 | 2917.27 | 6.3 |
| Example 3 | 2044.85 | 6.6 |
| Example 4 | 2136.13 | 7.1 |
| Example 5 | 2209.23 | 6.9 |
| Example 6 | 2688.69 | 6.7 |
| Example 7 | 2446.32 | 7.0 |
| Example 8 | 2375.42 | 6.9 |
| Comparative example 1 | 1668.96 | 14.2 |
| Comparative example 2 | 1015.27 | 14.5 |
| Comparative example 3 | 1573.14 | 13.9 |
| Comparative example 4 | 1792.31 | 13.5 |
| Comparative example 5 | 1519.21 | 12.2 |
| Comparative example 6 | 1647.34 | 12.7 |
| Comparative example 7 | 1768.23 | 12.8 |
| Comparative example 8 | 1554.68 | 11.9 |
As is apparent from the data in tables 1 and 2, the number of lateral branches, the length of the lateral branches and the biomass of the overground parts of the dragon fruit seedlings after the cultivation treatment of the plant nutrient solution (Amm +nit+Gly treatment) obtained according to the formula of the application are all obviously higher than those of the comparative example (Amm +nit treatment); in particular, the Amm +Nit+Gly (ammonium nitrogen+nitrate nitrogen+glycine) treatment in examples 1 to 3 showed a 1.8-fold increase in root length compared to the Amm +Nit (ammonium nitrogen+nitrate nitrogen) treatment in comparative examples 1 to 3, a significant decrease in root length compared to comparative examples 4 to 8, a significant increase in root length, and a significant decrease in root length, in accordance with the theory that root hair plays an important role in nutrient and moisture absorption, and in examples 4 to 8, a significant increase in root length compared to comparative examples 1 to 8, and a significant inhibition of root length. According to analysis, mainly because nitrogen with different forms is added into Hoagland nutrient solution, nitrogen application is beneficial to growth of root systems and branches of dragon fruits, NH4+ balance in cytoplasm can maintain growth of root hair top ends, and additionally added second ammonia source glycine can promote growth of root hair pairs, and the mechanism is that Reactive Oxygen Species (ROS) are regulated by ROPs to maintain a calcium ion concentration gradient in cytoplasm, and the calcium ion concentration gradient is important to root hair top end growth.
In summary, the second nitrogen source-glycine plant nutrient solution provided by the embodiment of the application obviously shortens the root system length and increases the root hair length of the root system of the dragon fruit, thereby affecting the absorption of nutrients and moisture and obviously improving the lateral branch number, the lateral branch length and the stem biomass of the dragon fruit seedling. As the root system of the dragon fruit is not longer and better in cultivation, the root needs to be raised, the application improves the attention to the root hair length, has important guiding significance to the field root-promoting cultivation technology and quality improvement and efficiency improvement, and simultaneously enables the research on the influence of different nitrogen forms on plant root hair to be further carried out.
It is to be understood that the above examples of the present application are provided by way of illustration only and not by way of limitation of the embodiments of the present application. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the application are desired to be protected by the following claims.
Claims (10)
1. The plant nutrient solution is characterized by comprising a basic nutrient solution, a first nitrogen source and a second nitrogen source, wherein the basic nutrient solution is Hoagland nutrient solution diluted by one time by adding water, and the first nitrogen source comprises NH (NH) 4 + -N and NO 3 And the second nitrogen source at least comprises glycine-N, and the molar concentration of the first nitrogen source and the second nitrogen source in the plant nutrient solution is 1.5-4 mM.
2. The plant nutrient solution of claim 1, wherein the second nitrogen source further comprises glutamate-N at a molar concentration of 2-3mM.
3. The plant nutrient solution of claim 1, wherein the plant nutrient solution comprisesThe basic nutrient solution comprises the following components in molar concentration: 0.5mM KH 2 PO 4 、1.25mM K 2 SO 4 、2.5mM CaCl 2 、1mM MgSO 4 ·7H 2 O、0.5mM EDTA-Fe、0.05mM FeSO 4 ·7H 2 O、0.05mM H 3 BO 3 、0.01mM MnSO 4 ·H 2 O、0.0008mM ZnSO 4 ·7H 2 O、0.0002mM CuSO 4 ·5H 2 O、0.0021mM Na 2 MoO 4 ·2H 2 O、0.0048mM KI。
4. The plant nutrient solution of claim 1, wherein the second nitrogen source is only glycine-N.
5. The plant nutrient solution of claim 1 or 4, wherein the NH 4 +-N、NO 3 The molar concentration of both the N and the glycine-N is 2-3mM.
6. The plant nutrient solution of claim 1 or 4, wherein the NH 4 +-N、NO 3 The molar concentrations of both-N and glycine-N were 2.5mM.
7. The plant nutrient solution of claim 1 or 4, wherein the NH 4 + -N、NO 3 - N, glycine-N is derived from (NH) 4 ) 2 SO 4 、KNO 3 And L-glycine.
8. Use of the plant nutrient solution of any one of claims 1 to 7 in cultivation of dragon fruits.
9. A preparation method of a plant nutrient solution is characterized in that a first nitrogen source and a second nitrogen source are added into a Hoagland nutrient solution diluted by 1 time by adding water, wherein the first nitrogen source comprises NH (NH) 4 + -N and NO 3 -N, said second nitrogen source comprising at least glycine-N.
10. The method for producing a plant nutrient solution as claimed in claim 9, wherein the NH 4 + -N、NO 3 - N, glycine-N are each represented by (NH) 4 ) 2 SO 4 、KNO 3 And L-glycine.
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| CN105651888A (en) * | 2016-01-08 | 2016-06-08 | 浙江大学 | Method for analyzing influence of environmental factors on contribution mechanism of amino-acid nutrition |
| CN111592429A (en) * | 2020-06-04 | 2020-08-28 | 漳州三炬生物技术有限公司 | Vegetable water culture nutrient solution containing amino acids as well as preparation method and application of vegetable water culture nutrient solution |
| CN112279738A (en) * | 2020-09-30 | 2021-01-29 | 广东施可达生物肥料集团有限公司 | Organic liquid fertilizer for pitaya and preparation method thereof |
| CN115010532A (en) * | 2022-04-20 | 2022-09-06 | 塔里木大学 | Nutrient solution containing organic components and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651888A (en) * | 2016-01-08 | 2016-06-08 | 浙江大学 | Method for analyzing influence of environmental factors on contribution mechanism of amino-acid nutrition |
| CN111592429A (en) * | 2020-06-04 | 2020-08-28 | 漳州三炬生物技术有限公司 | Vegetable water culture nutrient solution containing amino acids as well as preparation method and application of vegetable water culture nutrient solution |
| CN112279738A (en) * | 2020-09-30 | 2021-01-29 | 广东施可达生物肥料集团有限公司 | Organic liquid fertilizer for pitaya and preparation method thereof |
| CN115010532A (en) * | 2022-04-20 | 2022-09-06 | 塔里木大学 | Nutrient solution containing organic components and preparation method thereof |
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| 陈秀虎;杨敏;郑会龙;皮晓娟;陈新苗;: "化学物质对红心火龙果抗冻生理的影响", 北方园艺, no. 06, pages 30 - 32 * |
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