CN117143851A - 一种皮革软化用复合酶及其制备方法与应用 - Google Patents
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Abstract
本发明提供了一种皮革软化用复合酶,所述复合酶包括按质量份数计的:微生物酶蛋白酶5‑30;脂肪酶0.1‑1.5;防结块剂5‑10;钙螯合剂5‑10;PH稳定剂10‑20;惰性盐稀释剂30‑70,其中,所述微生物酶蛋白酶为来源于动物胰脏的蛋白酶。本发明所提供皮革软化用复合酶,基于微生物蛋白酶制得,通过微生物胰蛋白酶、脂肪酶、防结块剂、钙螯合剂、pH稳定剂和惰性盐稀释剂等复合组分的协同作用,能更有效的去除皮内的非结构蛋白和脂肪、促进酶的渗透和软化作用、进一步松散胶原纤维,因而能达到更理想的软化效果。
Description
技术领域
本发明涉及皮革加工技术领域,具体涉及一种基于微生物胰蛋白酶的皮革软化用复合酶制剂及其制备方法与应用。
背景技术
在皮革加工的准备工段中,需要用酶制剂处理脱灰后的裸皮,该过程被称为酶软化,其主要作用是去除制革无用之物,如表皮、白蛋白、球蛋白、黏蛋白和蛋白多糖等纤维间质,并进一步松散结构蛋白纤维,使裸皮的粒面更清洁、细腻,手感更柔软,为后续鞣制工段做准备。酶软化是制革过程最主要的工序之一,也是唯一不能被化学处理所代替的操作,其对成革质量的好坏具有决定性的作用。
一般情况下,裸皮的酶软化是在脱灰后pH 7-9的浴液中进行的。而胰蛋白酶作为一种主要作用于精氨酸和赖氨酸残基的高度专一性蛋白酶,其对动物皮结构蛋白的催化水解能力相对较弱,对皮胶原蛋白的破坏程度较小,往往作为裸皮软化的首选蛋白酶制剂,使得酶对脱灰裸皮的软化过程相对安全、可控。然而,市场上销售的胰蛋白酶主要来源是从动物胰腺组织中提取的动物胰蛋白酶,多年来的应用结果显示,其一直存在诸多缺陷:如动物胰腺原材料的短缺、生产成本较高,通过简单提取的工业胰蛋白酶组分复杂、异味重,不同厂家或同一厂家不同批次产品之间的差异性较大、产品稳定性较差,动物源的胰蛋白酶存在动物病毒的交叉感染和对人体免疫性的毒性等。动物胰蛋白酶的上述缺陷限制了制革过程裸皮酶软化技术的发展和应用,使得以动物胰蛋白酶为基础的皮革软化酶制剂质量不稳定,软化过程中存在皮粒面过度作用的风险。因此,需要开发新的裸皮酶软化策略以解决上述长期困扰制革行业发展的问题。
近年来,通过自然选育的微生物或基因工程菌发酵生产的胰蛋白酶代替动物胰蛋白酶成为一种趋势,发酵所得微生物胰蛋白酶与动物胰蛋白酶的氨基酸序列具有高度的同源性、相似的酶学特性及相同的底物专一性。并且,微生物生长迅速、培养成本低,通过基因工程和发酵代谢调控等手段对产酶微生物及发酵过程进行优化,能显著提高微生物发酵产酶的能力,并保持产品批次之间质量的稳定性。因此,随着微生物发酵技术和基因工程技术的快速发展,通过微生物发酵生产的微生物胰蛋白酶代替传统的动物胰蛋白酶在食品、医药、洗涤剂和生物燃料等领域取得了较大的进展,也为制革裸皮酶软化过程软化用酶制剂的选择提供了更多的可能性。
发明内容
为了解决上述问题,本发明提供了一种基于微生物胰蛋白酶的皮革软化用复合酶制剂及其制备方法与应用。
本发明的目的在于,提供一种皮革软化用复合酶,所述复合酶包括按质量份数计的:
其中,所述微生物酶蛋白酶为来源于由微生物或基因工程菌发酵得到的胰蛋白酶。
本发明用微生物胰蛋白酶代替传统的动物胰蛋白酶,在pH值6.0-9.0的范围内处理裸皮,实现软化效果。此外,裸皮内有一些脂腺和脂肪组织,单一的微生物胰蛋白酶只能对皮内的非结构蛋白有去除作用、对胶原纤维有一定的松散和破坏作用,而对脂肪无破坏和去除作用,而且皮内脂肪的存在也影响着酶制剂的渗透。因此,加入防结块剂、钙螯合剂、惰性盐稀释剂以辅助蛋白酶达到理想的软化效果,而PH稳定剂的加入是为了稳定软化过程中的PH值。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述微生物酶蛋白酶的活力(利用福林-酚法测定蛋白酶活力)不小于10000U/g,所述脂肪酶的活力不小于10000U/g。
在软化温度和PH的要求范围内选择达到活力的微生物蛋白酶以及脂肪酶。脂肪酶最大的特点应该是脱脂,减少或者消除表面活性剂的使用;脱脂更均匀、彻底,从而促进染料的吸收,脱脂废液中的油脂更易被分离、回收,环境负荷小。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述微生物酶蛋白酶包括由微生物或基因工程菌发酵得到的胰蛋白酶,其适作用PH值为6-10。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述脂肪酶包括来源于动物、植物或微生物的脂肪酶,其适作用PH值为5-11。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述防结块剂包括从木屑、米糠、淀粉、高岭土、硅铝酸钠和二氧化硅中选出的一种或多种。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述钙螯合剂包括从乙二胺四乙酸钠盐、多聚磷酸盐、焦磷酸盐和氮川三乙酸盐中选出的一种或多种。
本发明所提供的皮革软化用复合酶,还具有这样的特征,所述PH稳定剂包括从硼酸、磷酸氢二钠、柠檬酸钠、硫酸铵和氯化铵中选出的一种或多种。
本发明的另一目的在于,提供一种皮革软化用复合酶的制备方法,所述制备方法用于制备如上述任一项所述的基于微生物胰蛋白酶的皮革软化用复合酶。
本发明还提供了一种上述任一项所述的皮革软化用复合酶在以动物皮为原料的皮革生产过程中的脱灰后单独软化的应用。
本发明还提供了一种上述任一项所述的皮革软化用复合酶在以动物皮为原料的皮革生产过程中的与脱灰同浴软化的应用。
本发明所提供的皮革软化用复合酶同时适用于有铵盐脱灰体系和无铵盐脱灰体系。
脂肪酶最大的特点应该是脱脂,减少或消除表面活性剂的使用:脱脂更均匀、彻底,从而促进染料的吸收,脱脂废液中的油脂更易被分离、回收,环境负荷小。因为其脱脂效果,所以使得软化效果更好。
本发明的有益技术效果:
本发明所提供皮革软化用复合酶,基于微生物蛋白酶制得,通过微生物胰蛋白酶、脂肪酶、防结块剂、钙螯合剂、pH稳定剂和惰性盐稀释剂等复合组分的协同作用,能更有效的去除皮内的非结构蛋白和脂肪、促进酶的渗透和软化作用、进一步松散胶原纤维,因而能达到更理想的软化效果。
本发明所提供皮革软化用复合酶,通过微生物胰蛋白酶、脂肪酶与钙螯合剂、pH稳定剂的协同作用,同时适用于裸皮脱灰后单独软化或与裸皮脱灰同浴软化,均能达到理想的软化效果。
本发明所提供皮革软化用复合酶,采用产量高、成本低且质量稳定的微生物胰蛋白酶代替动物胰蛋白酶,解决了传统制革酶软化技术以动物胰蛋白酶为基础的皮革软化酶质量不稳定、软化过程存在皮粒面过度作用的风险等缺点,能保证成革质量的稳定性。
本发明所提供皮革软化用复合酶,适合以各种动物皮(如黄牛皮、水牛皮、奶牛皮、牦牛皮、猪皮、绵羊皮和山羊皮等)为原料的软化,适合各种用途皮革(如沙发革、鞋面革、服装革、包袋革、汽车坐垫革等)的生产过程。
具体实施方式
为了使本发明的目的及优点更加清楚明白,以下结合实施例对本发明进行进一步详细说明。应当理解,此处所描述的具体实施例仅仅用以解释本发明,并不用于限定本发明。本领域的技术人员可以根据上述本发明的内容作出一些非本质的改进和调整。所用试剂的来源、商品名以及有必要列出其组成成分者,均在首次出现时标明。
实施例一
取最适作用pH值为7.0-9.0,酶活力为3万单位/g的NB115微生物胰蛋白酶(四川达威科技股份有限公司)10kg,酶活力为10万单位/g的LKT脂肪酶(山东隆科特酶制剂有限公司)1kg;木屑5kg;乙二胺四乙酸二钠5kg;磷酸氢二钠5kg;柠檬酸钠5kg;硫酸铵10kg;硫酸钠50kg,混合均匀,制得皮革软化用复合酶制剂。
用上述实施例所制得的皮革软化用复合酶制剂对黄牛皮进行脱灰后单独软化处理,步骤如下:
取按常规方法浸水、脱毛、浸灰、片皮、脱灰处理后的头层黄牛皮100kg(以片皮后头层灰皮质量计),放入转鼓,加入水约50kg,调温至35℃,加入按上述配方配制的皮革软化用复合酶制剂1.0kg(或按上述复合酶的配方按同样的用量比例分别称取各组分后加入转鼓中),转动约60分钟左右,排液,水洗后按常规浸酸、铬鞣、复鞣、加脂和干燥等加工工序操作,得到实验样坯革。
对照实验,步骤如下:
取按常规方法浸水、脱毛、浸灰、片皮、脱灰处理后的头层黄牛皮100kg(以片皮后头层灰皮质量计),放入转鼓,加入水约50kg,调温至35℃,加入头层灰皮质量1%的商品动物胰蛋白酶软化剂,转动约60分钟左右,排液,水洗后按常规浸酸、铬鞣、复鞣、加脂和干燥等加工工序操作,得到对照坯革。
将上述实验样坯革和对照坯革按相同的常规工艺浸酸、铬鞣、复鞣、加脂、干燥和摔软后,测定所得坯革的柔软度,观察其粒面损伤程度并评价其整体感官性能,结果见表1。由表1可以看出,采用本发明的脱灰后单独软化用复合酶制剂软化处理后,所得成革的柔软度更高、粒面几乎无损伤且整体感官性能更好。
表1该实施例所提供的皮革软化用复合酶制剂与现有技术中所使用的动物胰蛋白酶的软化效果对比表
实施例二
取最适作用pH值为7.0-9.0,酶活力为2万单位/g的JD18微生物胰蛋白酶15kg,酶活力为10万单位/g的LKT脂肪酶(山东隆科特酶制剂有限公司)1kg;木屑9kg;乙二胺四乙酸二钠10kg;硼酸10kg;柠檬酸钠5kg;硫酸铵5kg;硫酸钠30kg,氯化钠15kg,混合均匀,制得皮革软化用复合酶制剂。
取按常规方法浸水、脱毛、浸灰和片皮处理后的头层黄牛皮100kg,放入转鼓,加入水约20kg,调温至33℃,加入按上述配方配制的皮革软化用复合酶制剂1.0kg(或按上述复合酶的配方按同样的用量比例分别称取各组分后加入转鼓中),转动约120分钟左右,排液,水洗后按常规浸酸、铬鞣、复鞣、加脂和干燥等加工工序操作,得到坯革。
采用上述实施例所提供的皮革软化用复合酶制剂对片皮后的头层灰皮进行脱灰、软化同浴处理,不仅能节省皮革加工时间、降低能耗、节约生产成本、提高生产效率,且所得成革的柔软度较高、粒面几乎无损伤、整体感官性能较好。
综上所述,本发明所提供皮革软化用复合酶,基于微生物蛋白酶制得,通过微生物胰蛋白酶、脂肪酶、防结块剂、钙螯合剂、pH稳定剂和惰性盐稀释剂等复合组分的协同作用,能更有效的去除皮内的非结构蛋白和脂肪、促进酶的渗透和软化作用、进一步松散胶原纤维,因而能达到更理想的软化效果。并且,同时适用于裸皮脱灰后单独软化或与裸皮脱灰同浴软化,均能达到理想的软化效果。
以上所述,仅为本发明的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。因此,本发明的保护范围应以权利要求的保护范围为准。
Claims (10)
1.一种皮革软化用复合酶,其特征在于,所述复合酶包括按质量份数计的:
其中,所述微生物酶蛋白酶为来源于由微生物或基因工程菌发酵得到的胰蛋白酶。
2.根据权利要求1所述的皮革软化用复合酶,其特征在于,所述微生物酶蛋白酶的活力不小于10000U/g,所述脂肪酶的活力不小于10000U/g。
3.根据权利要求1所述的皮革软化用复合酶,其特征在于,所述微生物酶蛋白酶包括由微生物或基因工程菌发酵得到的胰蛋白酶,其适作用PH值为6-10。
4.根据权利要求1所述的皮革软化用复合酶,其特征在于,所述脂肪酶来源于动物、植物或微生物,其适作用PH值为5-11。
5.根据权利要求1所述的皮革软化用复合酶,其特征在于,所述防结块剂包括从木屑、米糠、淀粉、高岭土、硅铝酸钠和二氧化硅中选出的一种或多种。
6.根据权利要求1所述的皮革软化用复合酶,其特征在于,所述钙螯合剂包括从乙二胺四乙酸钠盐、多聚磷酸盐、焦磷酸盐和氮川三乙酸盐中选出的一种或多种。
7.根据权利要求1所的皮革软化用复合酶,其特征在于,所述PH稳定剂包括从硼酸、磷酸氢二钠、柠檬酸钠、硫酸铵和氯化铵中选出的一种或多种。
8.一种皮革软化用复合酶的制备方法,其特征在于,所述制备方法用于制备如权利要求1-7任一项所述的基于微生物胰蛋白酶的皮革软化用复合酶。
9.如权利要求1-7任一项所述的皮革软化用复合酶在以动物皮为原料的皮革生产过程中的脱灰后单独软化的应用。
10.如权利要求1-7任一项所述的皮革软化用复合酶在以动物皮为原料的皮革生产过程中的与脱灰同浴软化的应用。
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