CN117106100B - Prrsv蛋白酶抑制剂的筛选方法和抑制效果评价方法 - Google Patents
Prrsv蛋白酶抑制剂的筛选方法和抑制效果评价方法 Download PDFInfo
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- CN117106100B CN117106100B CN202311360822.6A CN202311360822A CN117106100B CN 117106100 B CN117106100 B CN 117106100B CN 202311360822 A CN202311360822 A CN 202311360822A CN 117106100 B CN117106100 B CN 117106100B
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- 102100031056 Serine protease 57 Human genes 0.000 claims abstract description 62
- 101710197596 Serine protease 57 Proteins 0.000 claims abstract description 62
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 61
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- 241000700605 Viruses Species 0.000 abstract description 11
- 102000034287 fluorescent proteins Human genes 0.000 abstract description 9
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- 102000004169 proteins and genes Human genes 0.000 abstract description 6
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- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 50
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- 238000001514 detection method Methods 0.000 description 5
- 102000035195 Peptidases Human genes 0.000 description 4
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- 101800000535 3C-like proteinase Proteins 0.000 description 1
- 101800002396 3C-like proteinase nsp5 Proteins 0.000 description 1
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- 102000004190 Enzymes Human genes 0.000 description 1
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- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Plant Pathology (AREA)
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CN202311360822.6A CN117106100B (zh) | 2023-10-20 | 2023-10-20 | Prrsv蛋白酶抑制剂的筛选方法和抑制效果评价方法 |
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CN202311360822.6A CN117106100B (zh) | 2023-10-20 | 2023-10-20 | Prrsv蛋白酶抑制剂的筛选方法和抑制效果评价方法 |
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CN117106100A CN117106100A (zh) | 2023-11-24 |
CN117106100B true CN117106100B (zh) | 2024-02-06 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113564149A (zh) * | 2021-07-15 | 2021-10-29 | 西湖大学 | 一种三联体融合蛋白及其在病毒自剪切蛋白酶抑制剂活性评估和筛选时的应用 |
CN113699169A (zh) * | 2021-07-29 | 2021-11-26 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 一种荧光素酶报告基因iGLuc、基因系统及其应用 |
CN116377019A (zh) * | 2023-03-07 | 2023-07-04 | 浙江师范大学 | 基于fret和应激颗粒的病毒3c蛋白酶抑制剂双筛选方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2432676A1 (en) * | 2002-07-31 | 2004-01-31 | Robert Menard | Development of an in vivo functional assay for proteases |
CA2744675A1 (en) * | 2008-11-26 | 2010-06-03 | South Dakota State University | Identification of porcine reproductive and respiratory syndrome virus |
-
2023
- 2023-10-20 CN CN202311360822.6A patent/CN117106100B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113564149A (zh) * | 2021-07-15 | 2021-10-29 | 西湖大学 | 一种三联体融合蛋白及其在病毒自剪切蛋白酶抑制剂活性评估和筛选时的应用 |
CN113699169A (zh) * | 2021-07-29 | 2021-11-26 | 中国农业科学院哈尔滨兽医研究所(中国动物卫生与流行病学中心哈尔滨分中心) | 一种荧光素酶报告基因iGLuc、基因系统及其应用 |
CN116377019A (zh) * | 2023-03-07 | 2023-07-04 | 浙江师范大学 | 基于fret和应激颗粒的病毒3c蛋白酶抑制剂双筛选方法 |
Non-Patent Citations (6)
Title |
---|
Green fluorescent protein as a scaffold for intracellular presentation of peptides;Majid R. Abedi等;Nucleic Acids Research;第26卷(第2期);第 625、629 页右栏第 1 段 * |
HCV NS3/4A蛋白酶胞内荧光检测方法的建立;闫兴等;中国生物工程杂志;第32卷(第07期);第85-87页右栏第3段 * |
Structure and Cleavage Specificity of the Chymotrypsin- Like Serine Protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV);Xinsheng Tian等;J. Mol. Biol.;第392卷 * |
猪繁殖与呼吸综合征病毒NSP4切割天然免疫分子NEMO抑制I型干扰素的产生;张利杰等;中国预防兽医学报(第03期);169-173 * |
细胞水平新型冠状病毒SARS-CoV-2 3CL蛋白酶抑制剂筛选模型的建立;马铃等;药学学报(第09期);156-160 * |
闫兴等.HCV NS3/4A蛋白酶胞内荧光检测方法的建立.中国生物工程杂志.2012,第32卷(第07期),第85-87页右栏第3段. * |
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Address after: 230000, Room 101-1, 1st Floor, Building A4, National Health Big Data Industrial Park, Intersection of Xiyou Road and Kongquetai Road, High tech Zone, Hefei City, Anhui Province Patentee after: Hefei Baiyu Biotechnology Co.,Ltd. Country or region after: China Address before: Floor 4, Building 1, No. 527, Dani Line Bridge Section, Heshang Town, Xiaoshan District, Hangzhou City, Zhejiang Province, 310000 Patentee before: Hangzhou Baiyu Biotechnology Co.,Ltd. Country or region before: China |
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