CN117018088A - Extraction method and application of green tea soaking liquid - Google Patents

Abstract

The application provides an extraction method of green tea soaking liquid and application thereof in preparing medicines for treating gynecological inflammation. Compared with the traditional method, the green tea soaking liquid has the advantages of quick clinical effect, long maintenance time after healing, difficult reinfection and contribution to restoring the normal microecological balance of the genital tract.

Description

Extraction method and application of green tea soaking liquid

Technical Field

The application relates to an extraction method of green tea soaking liquid and application thereof in gynecological inflammation.

Background

Gynecological inflammation is a clinical problem of gynecology, recurrent attacks are difficult to cure, and traditional methods have historically used sterilization as a main purpose, and the used drugs kill pathogenic bacteria and beneficial bacteria; meanwhile, the repair process of mucous membrane is destroyed, and excessive medicine use causes beneficial bacteria not to quickly establish chemical protective barrier, so that the immune mechanism of genital tract is destroyed.

At present, international gynaecology and obstetrics generally recognizes the microecological balance adjustment theory of female genital tract infection, and the gynaecology and obstetrics treatment claims that the original colonization beneficial bacteria are not killed, so that the lactobacillus is promoted to grow, and a more effective treatment method is sought under the guidance of the microecological theory. The microecology theory refers to: each organ of the human body has own micro-ecological environment, and the disease of the organ should be adjusted according to the micro-ecological physiological environment of the organ, but not only the antibiotic medicine treatment, especially the treatment of female genital tract mucosa inflammation.

The method reduces the interference of the medicine on the microecology of the female genital tract, solves the dependence of clinic on the medicine, solves the medicine resistance problem of clinical gynecological genital tract mucosa inflammation, searches for a better treatment method, solves the actual problem, and has a new healthy and green value to recover the microecology of the female genital tract, thus being a general requirement of gynecological clinic.

According to the published research data, the green tea has the functions of preventing cancer and inhibiting pathological cell activities, and has the functions of inhibiting and resisting virus and bacterial infection when being externally used. For thousands of years, there have been many records about tea and tea medicine and health. In particular, tea is often used as a medicine in ancient times in China, and in medical treasury in China, tea is quite common to be used as a single or compound medicine. In addition, the tea mainly comprises the following components with the functions of diminishing inflammation, resisting bacteria and improving immunity:

A. the chlorophyll content in the tea leaves is about 0.6% of the thousand weight of the tea leaves, and the tea leaves are related to the quality of the tea leaves, and have the functions of resisting bacteria, diminishing inflammation, and the like.

B. The tea leaves are also rich in beta-carotene, and the tea leaves have the function of vitamin A, and one beta-carotene molecule can be converted into 2 molecules of vitamin A under the action of in-vivo enzymes. In addition, it has antioxidant effect, and can eliminate free radical in body, strengthen immunity, raise disease resistance of human body, etc.

C. The theaflavin is formed by oxidative condensation of tea polyphenol and derivatives thereof, is one of main physiological active substances of tea, and has proved by researches to have the function similar to the tea polyphenol and high practical value. The theaflavin is not only an effective free radical scavenger and antioxidant, but also has various physiological functions of resisting cancer, resisting mutation, inhibiting bacteria and virus, improving and treating cardiovascular and cerebrovascular diseases, treating diabetes and the like.

Disclosure of Invention

Aiming at the defects and actual demands of the prior art, the application provides an extraction method of green tea soaking liquid and application thereof in treating gynecological inflammation.

To achieve the purpose, the application adopts the following technical scheme:

in a first aspect, the present application provides a method of extracting green tea infusions, the method comprising the steps of:

step one, screening and cleaning.

And step two, sterilizing.

And thirdly, soaking in a temperature-controlled manner.

And step four, filtering and packaging.

The first step optionally comprises the steps of removing impurities, cleaning, and rapidly drying surface water for standby.

And step two, optionally placing the dried tea leaves in a high-temperature and high-pressure container, sterilizing at high temperature and high pressure, and transferring to the next link. Preferably, the high temperature and high pressure are at a temperature of 110-120 ℃, the pressure is 0.10-0.13MPa, the sterilization time is 10-30 minutes, and the materials are transported to the next step in a closed environment (without contact and sterile operation).

The third step further comprises the following steps:

adding purified water, heating to 100-140 ℃, reducing to about 76 ℃ after sterilization for 10-30 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking in water at about 76 ℃, strictly controlling the temperature difference between water and water to be plus or minus 1 ℃, and the ratio of tea to water to be 1:6-7. The constant water temperature of about 76 ℃ is controlled to be kept for soaking for a period of time, so that the active physiological ingredients in the tea leaves are prevented from being destroyed to the greatest extent.

And B, after the soaking for a period of time, the soaking temperature is reduced stepwise by about 7.2 degrees in each hour under a closed environment (a non-contact sterile operation), and finally the water temperature is reduced to about 40 degrees.

C, keeping constant water temperature at about 40 ℃ under sealing (non-contact aseptic operation), and continuously soaking for 3-14 hours to maximally absorb the effective components in the tea.

And D, under a closed environment (non-contact sterile operation), the soaking liquid passes through a filtering device, the concentrated tea liquid enters a closed container through a closed pipeline to obtain the greenish clean transparent green tea soaking liquid, and then the natural state is cooled to about 26 ℃ for standby.

Wherein, in the step A, the soaking period of time can be 30 minutes to 2 hours.

And step four, optionally, rapidly split charging and sealing in a closed environment (in a non-contact sterile operation), split charging into finished products with different specifications through a closed pipeline, and one person can use the product once in principle, so that cross infection in clinical use is prevented. The storage and transportation are carried out at low temperature, for example, 2-10 ℃, preferably 4-8 ℃.

In a second aspect, the present application provides the use of a green tea infusion as described in the first aspect in the manufacture of a medicament for the treatment of gynecological inflammation.

The application accords with the academic theory of the current latest authoritative expert scholars, and an approximate implementation scheme is not known at present for clinic.

The inventor is used as a clinical gynecologist to conduct clinical work for years, refers to a large amount of Chinese herbal medicine data in long-term clinical practice, accumulates abundant treatment experience of plant extracts on clinical diseases, discovers that green tea has anti-inflammatory, antibacterial and antiviral effects after continuous years of clinical trial and error observation and intensive research and screening, and confirms that green tea (the optimal Longjing tea) soaking liquid has remarkable curative effects on clinical treatment of gynecological genital tract infection inflammation.

In the third step, the control of the temperature difference is the optimal range obtained through the results of a large number of experiments, and the curative effect of the green tea soaking liquid obtained below or above the range of the application is obviously reduced. The green tea soaking liquid is prepared by soaking the green tea in water at a water temperature of 76 ℃ and then reducing the soaking temperature of about 7.2 ℃ to about 40 ℃ in a stepwise manner in each hour, keeping the constant water temperature at about 40 ℃ and continuously and fully soaking for 6 hours, and the obtained green tea soaking liquid has the best treatment effect according to the temperature and the temperature difference. Therefore, the soaking temperature and temperature difference data in the step three of the green tea soaking liquid extraction method are one of the important application points of the application.

On the other hand, the concentration ratio data of the tea and the water used in the step three is also one of the important application points of the application. The specific selection tea-to-water ratio of the application is 1:6-7, the effect of the green tea soaking solution obtained below or above the above range of the present application is significantly reduced.

Compared with the prior art, the application has the following beneficial effects:

(1) The green tea soaking liquid has obvious clinical curative effect on gynecological mucosa inflammation and obvious treatment advantage compared with the traditional treatment;

(2) No toxic or side effect of chemical medicines, and undoubtedly safety;

(3) Through long-term observation of clinical use, the medicine dependence is completely absent;

(4) Compared with the traditional method, the curative effect of the female genital tract infection is improved by more than 30 times by clinical observation, the time used for treatment is shortened by more than 80 percent, the maintenance time is long after the clinical onset of action is quick, and the curative effect of difficult reinfection is particularly remarkable;

(5) The coverage rate of gynecological and urinary multi-organ inflammatory diseases of female patients at all ages is high, the repeatability is stable, and the practicability is high;

(6) The clinical genital tract inflammation signs disappear rapidly, the body feeling of the patient does not have any discomfort or irritation in the treatment process, and the clinical compliance of the genital tract inflammation patient is greatly improved;

(7) The process is simple and convenient in material taking, the Chinese is a tea origin, the tea belongs to the field of traditional Chinese medicines from ancient food and medicine, a great amount of data is recorded from ancient, the tea is both a drink and a medicinal material, the tea is inexhaustible, and the source is very wide;

(8) The application has the advantages of clinical in-depth and expanded research application value, and clinical observation shows that the application has specific curative effect on HPV infection elimination;

(9) The method meets the latest research theory direction of the current clinical gynecology authoritative expert scholars, reduces the use of harmful chemical medicines mainly used for sterilization, and adjusts female genital tract infection diseases by a healthy microecological balance method, thereby restoring the normal microecology of the genital tract.

Drawings

FIG. 1 is a process flow diagram of the present application.

Detailed Description

The technical means adopted by the application and the effects thereof are further described below with reference to the examples and the attached drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting thereof.

The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or apparatus used were conventional products commercially available through regular channels, with no manufacturer noted.

Example 1

In the embodiment, longjing tea is selected as a raw material of green tea. The embodiment is a method for extracting the concentrated solution of the Longjing tea, which comprises the following steps:

step one, selecting Longjing tea (tea in spring and summer) produced in Zhejiang, removing impurities, cleaning, and rapidly drying surface water for later use.

Step two, sterilization treatment: and (3) placing the dried tea leaves in a high-temperature and high-pressure container, and transferring to the next link, namely soaking, under the conditions of high temperature of 110 ℃, pressure of 0.12MPa and time of 10 minutes under a closed environment (non-contact sterile operation).

Step three, soaking and taking active ingredients:

a, a sealed and turnover 360-degree sterile tank is adopted, a glass fiber reinforced plastic material is adopted as a liner of the sterile tank, and the tea is not suitable for long-term contact with a metal material during soaking. Adding purified water according to a set amount, heating to 120 ℃, cooling to 76 ℃ after sterilization for 20 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking in water at 76 ℃, strictly controlling the temperature difference of water to +/-1 ℃, and the ratio of tea to water to be 1:6.7. the constant water temperature at 76 ℃ is controlled to be kept for soaking for one hour, so that the active physiological components in the tea are prevented from being destroyed to the greatest extent.

B, from about the second hour to about the sixth hour in a closed environment (no-contact aseptic operation), the soaking temperature is stepped down by about 7.2 degrees per hour, and finally the water temperature is lowered to 40 degrees.

And C, keeping the constant water temperature at 40 ℃ under the sealing (non-contact aseptic operation), and continuously soaking for 6 hours to maximally absorb the effective components in the tea.

And D, under a closed environment (non-contact sterile operation), enabling the soaking liquid to enter a closed container through a three-time filtering device, enabling the concentrated tea liquid to enter the closed container through a closed pipeline to obtain the light green clean transparent Longjing tea soaking liquid, and then cooling to about 26 ℃ in a natural state for later use.

Step four, packaging the products in a sub-package mode: the packaging and sealing are carried out rapidly under the closed environment (non-contact aseptic operation), and finished products with different specifications are packaged through a closed pipeline, so that the packaging machine can be used once in one person in principle, and the cross infection of clinical use is prevented. The finished product package is a brown glass bottle aluminum foil sealed package, and the shelf life is 12 months or 18 months. Warehouse-in storage at 5-8 deg.C, low-temperature storage and transportation.

Example 2

The difference between this embodiment and embodiment 1 is only the above-mentioned step three, which includes the following steps:

a, a sealed and turnover 360-degree sterile tank is adopted, a glass fiber reinforced plastic material is adopted as a liner of the sterile tank, and the tea is not suitable for long-term contact with a metal material during soaking. Adding purified water according to a set amount, heating to 100 ℃, cooling to 70 ℃ after sterilization for 10 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking in 70 ℃ water temperature, strictly controlling the water temperature difference to +/-1 ℃, and the ratio of tea to water to be 1:6.7. the constant water temperature at 70 ℃ is controlled to be kept for soaking for one hour, so that the active physiological components in the tea are prevented from being destroyed to the greatest extent.

And B, after the soaking for a period of time, the soaking temperature is reduced stepwise to 7 ℃ in each hour under a closed environment (non-contact sterile operation), and finally the water temperature is reduced to 37 ℃.

And C, keeping the constant water temperature at 37 ℃ under sealing (non-contact aseptic operation), and continuously soaking for 3 hours to maximally absorb the effective components in the tea.

And D, under a closed environment (non-contact sterile operation), enabling the soaking liquid to enter a closed container through a three-time filtering device, enabling the concentrated tea liquid to enter the closed container through a closed pipeline to obtain the light green clean transparent Longjing tea soaking liquid, and then cooling to about 25 ℃ in a natural state for later use.

Example 3

The difference between this embodiment and embodiment 1 is only the above-mentioned step three, which includes the following steps:

a, a sealed and turnover 360-degree sterile tank is adopted, a glass fiber reinforced plastic material is adopted as a liner of the sterile tank, and the tea is not suitable for long-term contact with a metal material during soaking. Adding purified water according to a set amount, heating to 140 ℃, cooling to 80 ℃ after sterilization for 30 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking in 80 ℃ water temperature, strictly controlling the water temperature difference to +/-1 ℃, and the ratio of tea to water to be 1:6.7. the constant water temperature of 80 ℃ is controlled to be kept for soaking for one hour, so that the active physiological components in the tea are prevented from being destroyed to the greatest extent.

And B, after the soaking for a period of time, the soaking temperature is reduced stepwise by 8 ℃ in each hour under a closed environment (non-contact sterile operation), and finally the water temperature is reduced to 42 ℃.

And C, keeping the constant water temperature at 42 ℃ under sealing (non-contact aseptic operation), and continuously soaking for 14 hours to maximally absorb the effective components in the tea.

And D, under a closed environment (non-contact sterile operation), enabling the soaking liquid to enter a closed container through a three-time filtering device, enabling the concentrated tea liquid to enter the closed container through a closed pipeline to obtain the light green clean transparent Longjing tea soaking liquid, and then cooling to about 37 ℃ in a natural state for later use.

Example 4

The difference between this example and example 1 is only the tea to water ratio in step three, 1:6.

example 5

The difference between this example and example 1 is only the tea to water ratio in step three, 1:7.

comparative example 1

Comparative example 1 differs from example 1 only in the above-described step three, comparative example 1 step three comprising the steps of:

a, a sealed and turnover 360-degree sterile tank is adopted, a glass fiber reinforced plastic material is adopted as a liner of the sterile tank, and the tea is not suitable for long-term contact with a metal material during soaking. Adding purified water according to a set amount, heating to 120 ℃, cooling to 69 ℃ after sterilization for 20 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking with 69 ℃ water temperature, strictly controlling the water temperature difference to +/-1 ℃, and the ratio of tea to water to be 1:6.7. the constant water temperature of 69 ℃ is controlled to be kept for soaking for one hour, so that the active physiological components in the tea are prevented from being destroyed to the greatest extent.

And B, after the soaking for a period of time, the soaking temperature is reduced stepwise by 6.5 ℃ in each hour under a closed environment (non-contact sterile operation), and finally the water temperature is reduced to 35 ℃.

And C, keeping the constant water temperature at 35 ℃ under the sealing (non-contact aseptic operation), and continuously soaking for 3 hours, so as to take the effective components in the tea to the maximum extent.

And D, under a closed environment (non-contact sterile operation), enabling the soaking liquid to enter a closed container through a three-time filtering device, enabling the concentrated tea liquid to enter the closed container through a closed pipeline to obtain the light green clean transparent Longjing tea soaking liquid, and then cooling to about 25 ℃ in a natural state for later use.

Comparative example 2

Comparative example 2 differs from example 1 only in the above-described step three, comparative example 2 step three comprising the steps of:

a, a sealed and turnover 360-degree sterile tank is adopted, a glass fiber reinforced plastic material is adopted as a liner of the sterile tank, and the tea is not suitable for long-term contact with a metal material during soaking. Adding purified water according to a set amount, heating to 120 ℃, cooling to 81 ℃ after sterilization for 20 minutes, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking in water at 81 ℃, strictly controlling the water temperature difference to +/-1 ℃, and the ratio of tea to water to be 1:6.7. the constant water temperature at 81 ℃ is controlled to be kept for soaking for one hour, so that the active physiological components in the tea are prevented from being destroyed to the greatest extent.

And B, after the soaking for a period of time, the soaking temperature is reduced stepwise by 8 ℃ in each hour under a closed environment (non-contact sterile operation), and finally the water temperature is reduced to 45 ℃.

And C, keeping the constant water temperature at 45 ℃ under the sealing (non-contact aseptic operation), and continuously soaking for 3 hours, so as to take the effective components in the tea to the maximum extent.

And D, under a closed environment (non-contact sterile operation), enabling the soaking liquid to enter a closed container through a three-time filtering device, enabling the concentrated tea liquid to enter the closed container through a closed pipeline to obtain the light green clean transparent Longjing tea soaking liquid, and then cooling to about 25 ℃ in a natural state for later use.

Comparative example 3

Comparative example 3 differs from example 1 only in the tea to water ratio in step three above, comparative example 3 tea to water ratio 1:5.8.

comparative example 4

Comparative example 4 differs from example 1 only in the tea to water ratio in step three above, comparative example 4 tea to water ratio 1:7.2.

effect verification example

Please describe the method of effect verification in detail: such as subject source, subject selection criteria, number of subjects, grouping, method of administration (including dosage, frequency of use, site method, etc.), result determination method or index, etc.

The results may be presented in the form of the following table.

Test source: outpatient randomization.

Subject selection criteria: colpitis mycotica. Visual inspection: the white bean dreg-like leucorrhea spreads over the vagina;

auxiliary inspection: mould (+) in the leucorrhea convention, or mould(s) (if present).

The number of subjects and the grouping: 90 humans from subject; the animals were randomized into 9 groups of 10 persons each.

The application method comprises the following steps: every person washes once a day, 200ML each time, fully washes the vagina, and washes for 3 days continuously.

And (3) result judgment: after 3 days, visual observation: the white bean dreg-like leucorrhea in the vagina disappears.

Auxiliary inspection: mold (-) in the white band convention, or mold (none).

Grouping	Cure rate
		Example 1	99％
Example 2	95％
		Example 3	90％
Example 4	50％
		Example 5	60％
Comparative example 1	70％
		Comparative example 2	75％
Comparative example 3	40％
		Comparative example 4	60％

From comparison of the experimental results of comparative examples 1-2 and examples 1-3, it is apparent that the effects of the soaking temperature and the temperature difference data on the curative effects of the green tea soaking liquid are significantly reduced, compared with the range used in examples 1-3. Wherein as in example 1, soaking is carried out by 76 ℃ water temperature, then the soaking temperature is reduced to about 40 ℃ in a step-like manner by about 7.2 ℃ in each hour, then the constant water temperature is kept at about 40 ℃, soaking is continued for 6 hours, and the obtained green tea soaking liquid has the best treatment effect.

From comparison of the experimental results of comparative examples 3 to 4 with those of examples 1 and examples 4 to 5, it is evident that the concentration ratio data of tea to water used in the third step has a significant effect on the curative effect of the green tea steep. The specific selection tea-to-water ratio of the application is 1:6-7, the effect of the green tea soaking solution obtained below or above the above range of the present application is significantly reduced.

The applicant states that the detailed method of the present application is illustrated by the above examples, but the present application is not limited to the detailed method described above, i.e. it does not mean that the present application must be practiced in dependence upon the detailed method described above. It should be apparent to those skilled in the art that any modification of the present application, equivalent substitution of raw materials for the product of the present application, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present application and the scope of disclosure.

Claims (8)

1. An extraction method of green tea soak solution, the extraction method comprising the steps of:

step one, screening and cleaning, namely removing impurities from green tea leaves, and cleaning;

step two, sterilizing, namely placing the dried tea leaves in a high-temperature high-pressure container, sterilizing at high temperature and high pressure, and transferring to the next link;

step three, soaking at a controlled temperature;

step four, filtering and packaging;

wherein, step three further comprises the following steps:

adding purified water, heating and sterilizing, then cooling to 70-80 ℃, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking with water temperature of 70-80 ℃, strictly controlling the temperature difference between water and water to be plus or minus 1 ℃, and the ratio of tea to water to be 1:6-7; controlling and keeping the constant water temperature of 70-80 ℃ for soaking for a period of time;

and B, after the soaking for a period of time, the soaking temperature is reduced stepwise by 7-8 ℃ in a closed environment (in a non-contact sterile operation) for each hour, and finally the water temperature is reduced to 37-42 ℃.

C, keeping constant water temperature at 37-42 ℃ under sealing (non-contact aseptic operation), and continuously soaking for 3-14 hours;

and D, under the closed environment (non-contact sterile operation), the soaking liquid passes through a filtering device, and the concentrated tea liquid enters a closed container through a closed pipeline to obtain the green tea soaking liquid.

2. The extraction method of green tea steep as claimed in claim 1, wherein in step a, the soaking period is 30 minutes to 2 hours;

preferably, the first step comprises the steps of removing impurities, cleaning, and rapidly drying surface water for standby;

preferably, the high temperature and high pressure in the second step are at a temperature of 110-120 ℃, the pressure is 0.10-0.13MPa, the sterilization time is 10-30 minutes, and then the materials are transported to the next step in a closed environment (without contact and sterile operation).

3. The method for extracting green tea steep according to any one of claims 1-2, wherein the fourth step comprises the steps of rapidly packaging and sealing in a closed environment (non-contact aseptic operation), and packaging into finished products of different specifications through a closed pipeline.

4. A method of extracting green tea extract as claimed in any one of claims 1 to 3, further comprising the step of storing and transporting at a low temperature, for example 2-10 degrees, preferably 4-8 degrees, after step four.

5. The method for extracting green tea steep according to any one of claims 1-4, wherein in step a the tea to water ratio is preferably 1:6.7.

6. The extraction method of green tea steep according to any one of claims 1-5, wherein step three specifically comprises the steps of:

adding purified water, heating and sterilizing, then cooling to 76 ℃, placing sterilized tea in a closed tank under a closed environment (non-contact aseptic operation), soaking with water temperature of 76 ℃, strictly controlling the temperature difference between water and water to be plus or minus 1 ℃, and the ratio of tea to water to be 1:6.7; controlling and keeping the constant water temperature at 76 ℃ for soaking for one hour;

and B, the soaking temperature is reduced stepwise by 7.2 ℃ in each hour under a closed environment (a non-contact sterile operation), and finally the water temperature is reduced to 40 ℃.

C, keeping constant water temperature at 40 ℃ under sealing (non-contact aseptic operation), and continuously soaking for 6 hours;

and D, under the closed environment (non-contact sterile operation), the soaking liquid passes through a filtering device, and the concentrated tea liquid enters a closed container through a closed pipeline to obtain the green tea soaking liquid.

7. A green tea extract as claimed in any one of claims 1 to 6.

8. The use of the green tea infusion as claimed in claim 7 in the manufacture of a medicament for the treatment of gynecological inflammation.