CN116982555B - Method for fast rooting and strengthening seedlings of lavender - Google Patents
Method for fast rooting and strengthening seedlings of lavender Download PDFInfo
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- CN116982555B CN116982555B CN202310985887.3A CN202310985887A CN116982555B CN 116982555 B CN116982555 B CN 116982555B CN 202310985887 A CN202310985887 A CN 202310985887A CN 116982555 B CN116982555 B CN 116982555B
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- 244000178870 Lavandula angustifolia Species 0.000 title claims abstract description 44
- 235000010663 Lavandula angustifolia Nutrition 0.000 title claims abstract description 44
- 239000001102 lavandula vera Substances 0.000 title claims abstract description 43
- 235000018219 lavender Nutrition 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000005728 strengthening Methods 0.000 title claims abstract description 13
- 239000005556 hormone Substances 0.000 claims abstract description 11
- 229940088597 hormone Drugs 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 239000001963 growth medium Substances 0.000 claims abstract description 8
- 238000010008 shearing Methods 0.000 claims abstract description 6
- 239000000758 substrate Substances 0.000 claims abstract description 6
- 238000005286 illumination Methods 0.000 claims description 27
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 11
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000005760 Difenoconazole Substances 0.000 claims description 6
- BQYJATMQXGBDHF-UHFFFAOYSA-N difenoconazole Chemical compound O1C(C)COC1(C=1C(=CC(OC=2C=CC(Cl)=CC=2)=CC=1)Cl)CN1N=CN=C1 BQYJATMQXGBDHF-UHFFFAOYSA-N 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 239000005869 Pyraclostrobin Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000003415 peat Substances 0.000 claims description 3
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 239000000243 solution Substances 0.000 description 17
- 241000196324 Embryophyta Species 0.000 description 13
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 208000006278 hypochromic anemia Diseases 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000207923 Lamiaceae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000000077 insect repellent Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 230000001105 regulatory effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for rapid rooting and seedling strengthening of lavender, which comprises the following steps: 1) Explant selection: selecting Lavender seed seedlings for pretreatment, and shearing Lavender new branch stem segments after 1-2 days; 2) Rooting and strengthening seedlings: removing the lower leaves of the sheared stem segments, reserving 2-4 leaves at the upper end, then inserting the leaves into a culture medium containing sugar-free culture solution and hormone, and transplanting after culturing for 20-30 days by adjusting culture conditions; 3) Transplanting and planting: and (5) performing pretreatment on the cultured seedlings for 1-2 days, and then transplanting or planting. The young top or stem segment of the lavender seedling or potted seedling is used as an explant, the stem segment is cut and inserted into a substrate of sugar-free culture solution for rooting and hardening off through pretreatment of the explant, the propagation coefficient is high, the rooting rate is high, the excellent characteristics of the seedling can be kept, the offspring can keep high consistency, factory production of the lavender seedling can be realized, and the large-scale planting and production of the lavender can be promoted.
Description
Technical Field
The invention relates to the technical field of lavender culture, in particular to a method for rapidly rooting and strengthening seedlings of lavender.
Background
Lavender (Lavandula angustifolia Mill.) is Labiatae, lavender genus half shrub or dwarf shrub, and the whole plant contains aromatic smell, and is an international natural spice plant; lavender is native to the Mediterranean coast and cultivated in Europe, asia, and oceangoin; the region of the Yi plow in Xinjiang has become the largest planting production base in China and even Asia. Lavender leaves and flower colors are elegant, can be used for garden flower diameter cluster planting or strip planting, and can also be used for potted plant ornamental; the essential oil extracted from the lavender flower batting has the effects of dispelling cold, tonifying stomach, regulating brain, eliminating dampness, relieving pain and the like, is commonly used as a raw material for aromatic, insect repellent and essence preparation, is widely used in various industries such as medicine health care, cosmetics, food and the like, and has very high social and economic benefits. Lavender planting has also driven the development of other related industries, and has also played a great role in the development of regional economy.
The propagation method of the lavender mainly comprises sowing, root separation, cutting and tissue culture, the germination rate of the lavender seeds is low, the genetic characteristics and the characters are unstable, the excellent quality characters of the original seedlings can be well maintained by adopting the sowing, the tissue culture and the cutting in production, but the rooting is poor, the seedling rate and the transplanting survival rate are not high. In production, root-division propagation is mainly adopted at present, but seedling degradation and disease and pest aggravation are easily caused by root division, and the propagation coefficient of root-division propagation is not high, so that the requirements of large-scale planting and industrial production are hardly met.
Disclosure of Invention
The invention aims to solve the technical problems of poor tissue culture and cuttage rooting, low transplanting survival rate and low propagation coefficient in the prior art by providing a method for rapidly rooting and strengthening lavender seedlings.
In order to achieve the above purpose, the invention provides a method for rapid rooting and seedling strengthening of lavender, which comprises the following steps:
1) Explant selection: selecting Lavender seed seedlings for pretreatment, and shearing Lavender new branch stem segments after 1-2 days;
2) Rooting and strengthening seedlings: removing the lower leaves of the sheared stem segments, reserving 2-4 leaves at the upper end, then inserting the leaves into a culture medium containing sugar-free culture solution and hormone, and transplanting after culturing for 20-30 days by adjusting culture conditions;
3) Transplanting and planting: and (5) performing pretreatment on the cultured seedlings for 1-2 days, and then transplanting or planting.
Further, in the step 1), 50-100mg/L of difenoconazole or 30-80mg/L of compound medicines containing difenoconazole, pyraclostrobin and the like are sprayed on the leaf surfaces of the selected lavender seedlings for pretreatment, and 2-4cm of stem segments are cut after 1-2 days.
Further, the sugar-free culture solution in the step 2) is 1/2MS culture solution without sucrose, and the hormone is IBA (indolebutyric acid) of 0.1-0.2 mg/L.
Further, the sugar-free culture solution in the step 2) is a prepared nutrient solution, and the prepared nutrient solution is a ternary compound fertilizer aqueous solution containing 0.1-0.3%; the hormone is IBA (indolebutyric acid) of 0.1-0.2 mg/L.
Further, the hormone may also be NAA (naphthalene acetic acid) of 0.1 to 0.3 mg/L.
Further, the culture medium in the step 2) is vermiculite, perlite, sand and peat soil; the volume ratio of the sugar-free nutrient solution to the matrix is (600-800) ml: (1000-1500) ml.
Further, after the lavender stem in the step 2) is inserted into the substrate, the lavender stem is pre-cultured in a culture room with the temperature of 20-23 ℃ and the illumination time of 14-16h per day and the illumination intensity of 2000-5000lx for 1-2 days, the temperature is adjusted to 23-25 ℃, the illumination intensity is adjusted to 6000-10000lx, and the illumination time per day is adjusted to 14-16h.
Further, the concentration of CO 2 in the culture room is adjusted to be 1000-2000 PPm, and after the air supply is stopped for 20-30min every 15min in the illumination time, the air supply is stopped for 15-min again, and the steps are repeated; the air supply amount is gradually increased by 200ml/min every two days until 1600-2000ml/min.
Further, the seedlings cultured in the step 3) are opened to a culture box cover, the temperature of a culture chamber is 23-27 ℃, the illumination intensity of the culture chamber is 8000-12000lx, the illumination time of the culture chamber is 14-16h each day, the concentration of CO 2 in the culture chamber is 1000-2000 PPm, and transplanting and planting are carried out after 1-2 days of culture.
Compared with the prior art, the invention has the following beneficial effects: the method takes the young top end or stem section of the lavender seedling or the potted seedling as an explant, and cuts the stem section or the top end to be inserted into a substrate of sugar-free culture solution for rooting and hardening off through pretreatment of the explant, so that the method has the advantages of high propagation coefficient, high rooting rate, simple and convenient operation, and can keep excellent characteristics of the seedling, keep high consistency of offspring, realize factory production of the lavender seedling, and facilitate large-scale cultivation and production of the lavender.
Detailed Description
The following describes the invention in more detail. The description of these embodiments is provided to assist understanding of the present invention, but is not intended to limit the present invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
The preferred embodiment of the invention provides a method for fast rooting and strengthening lavender seedlings, which comprises the following steps:
1) Explant selection: selecting lavender seed seedling or potted seedling leaf surface to spray pesticide for pretreatment, and shearing a new top end or stem section of 2-4cm after 1-2 days;
2) Rooting and strengthening seedlings: removing the cut top or lower leaves of the stem segment, reserving 2-4 leaves at the upper end, then inserting the leaves into a culture medium containing sugar-free culture solution and hormone, and transplanting after culturing for 20-30 days by adjusting culture conditions;
3) Transplanting and planting: and (5) performing pretreatment on the cultured seedlings for 1-2 days, and then transplanting or planting.
Preferably, in the step 1), 50-100mg/L of difenoconazole or 30-80mg/L of compound medicines containing difenoconazole, pyraclostrobin and the like are sprayed on the leaf surfaces of selected lavender seedlings for pretreatment, and 2-4cm of stem segments are cut after 1-2 days.
Preferably, the sugar-free culture solution in the step 2) is a sucrose-free 1/2MS culture solution, and the hormone is IBA (indolebutyric acid) of 0.1-0.2 mg/L.
Preferably, the sugar-free culture solution in the step 2) is a prepared nutrient solution, and the prepared nutrient solution is a ternary compound fertilizer aqueous solution containing 0.1% -0.3%; the hormone is IBA (indolebutyric acid) of 0.1-0.2 mg/L.
Preferably, the hormone may also be NAA (naphthalene acetic acid) in the range of 0.1 to 0.3 mg/L.
Preferably, the culture medium in the step 2) is vermiculite, perlite, sand or peat soil; the volume ratio of the sugar-free nutrient solution to the matrix is (600-800) ml: (1000-1500) ml.
Preferably, after the lavender stem in the step 2) is inserted into the substrate, the lavender stem is pre-cultured in a culture room with the temperature of 20-23 ℃ and the illumination time of 14-16 h/day and the illumination intensity of 2000-5000lx for 1-2 days, the temperature is adjusted to 23-25 ℃, the illumination intensity is adjusted to 6000-10000lx, and the illumination time of each day is adjusted to 14-16h.
Preferably, the concentration of CO 2 in the culture room is adjusted to 1000-2000 PPm, and the air supply is stopped for 20-30min every 15min in the illumination time, and then 15-min is supplied again, and the above steps are repeated; then the air supply amount is gradually increased by 200 ml/min every two days until 1600-2000ml/min
Preferably, the seedlings cultured in the step 3) are opened to a culture box cover, the temperature of a culture chamber is 23-27 ℃, the illumination intensity of the culture chamber is 8000-12000lx, the illumination time of the culture chamber is 14-16h each day, the concentration of CO 2 in the culture chamber is 1000-2000 PPm, and transplanting and planting are carried out after 1-2 days of culture.
Example 1:
1) Explant selection: selecting lavender seeds for seedling planting or potted seedlings, using 50-100mg/L of difenoconazole file, spraying leaf surfaces for pretreatment, and shearing 2-4cm of new-born top ends or stem segments after spraying for 1-2 days.
2) Rooting and strengthening seedlings: removing the lower leaf of the cut top or stem segment, retaining 2-4 leaves at the upper end, inserting into 1/2MS culture solution containing sugar, and adding into 0.1-0.2 mg/L IBA vermiculite culture medium.
The temperature is adjusted to 23-25 ℃ after the pre-culture is carried out for 1-2 days at 20-23 ℃ under the illumination of 14-16 h/day with the illumination intensity of 2000-5000lx, the illumination intensity is adjusted to 6000-10000lx, the illumination time is 14-16 h/day, the concentration of CO 2 in the culture chamber is adjusted to 1000-2000 PPm, and air containing CO 2 is input into the culture box; after the air is input every 15min for air supply, the air supply is stopped for 45 min and then 15min is performed again, and the steps are repeated; and gradually increasing the air supply amount by 100ml/min every other day until 1400-1800ml/min, culturing for 7-10 days until the root system is developed, growing the plant vigorously, and transplanting and planting after continuous culturing for 20-30 days.
Control group 1 (hormone-added, no CO 2 -containing gas), control group 2 (no hormone-added, no CO 2 -containing gas) and control group 3 (no hormone-added, no CO 2 -containing gas), were cultured at the same temperature and under the same conditions, and growth conditions were observed periodically; new roots do not grow after culturing for 7-10 days, and the plant growth vigor is poor; compared with a control group, the plant rooting time is shorter (new rooting is generated in 7 days), and the rooting time of the control group plant is as long as 15-30 days; the rooting rate of the plant reaches 97.6% after 10 days, the average rooting number of each plant is 5.8, the plant is green, the growth vigor is good, and the seedling rate is 96.3% after 30 days (shown in table 1).
TABLE 1 culture effects of the invention and control groups
| Rooting period (Tian) | Rooting rate on day 10 (%) | Rooting number (bars) of each plant on day 10 | Growth after 10 days | Rate of seedlings after 30 days (%) | |
| Inventive group | 7 | 97.6 | 5.8 | Green leaves and good growth vigor | 96.3 |
| Control group 1 | 15 | 0 | 0 | Leaf chlorosis and partial plant browning and death | 58.1 |
| Control group 2 | 18 | 0 | 0 | Leaf chlorosis and partial plant browning and death | 62.5 |
| Control group 3 | 25 | 0 | 0 | Leaf chlorosis and most plants brown and die | 47.9 |
3) Transplanting and planting: the cultured seedling is pretreated by opening a culture box cover, the temperature is 23-27 ℃, the illumination intensity is 8000-12000lx, the illumination is 14-16 h/day, the CO2 concentration in the culture chamber is kept to be 1000-2000 ppm, and the transplanting is carried out after the culture is carried out for 1-2 days.
From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: the method takes the tender top end or the stem section of the lavender seedling or the potted seedling as an explant, and cuts the stem section or the top end to be inserted into a substrate of sugar-free culture solution for rooting and hardening off through the pretreatment of the explant, so that the propagation coefficient is high, the rooting rate is high, the operation is simple and convenient, the excellent characteristics of the seedling can be maintained, the high consistency of offspring can be maintained, the factory production of the lavender seedling can be realized, and the large-scale planting and the production of the lavender can be promoted.
The embodiments of the present invention have been described in detail above, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, and yet fall within the scope of the invention.
Claims (1)
1. The method for rapidly rooting and strengthening lavender seedlings is characterized by comprising the following steps of:
1) Explant selection: selecting Lavender seed seedlings for pretreatment, and shearing Lavender new branch stem segments after 1-2 days;
2) Rooting and strengthening seedlings: removing the lower leaves of the sheared stem segments, reserving 2-4 leaves at the upper end, then inserting the leaves into a culture medium containing sugar-free culture solution and hormone, and transplanting after culturing for 20-30 days by adjusting culture conditions;
3) Transplanting and planting: pretreating the cultured seedlings for 1-2 days, and then transplanting or planting;
The method comprises the steps of 1) spraying compound medicines containing difenoconazole file and pyraclostrobin on leaf surfaces of selected lavender seedlings for pretreatment, and shearing stem segments of 2-4cm after 1-2 days;
The sugar-free culture solution in the step 2) is 1/2MS culture solution without sucrose; the hormone is 0.1-0.2 mg/L indolebutyric acid (IBA);
The culture medium in the step 2) is vermiculite, perlite, sand and peat soil; the volume ratio of the sugar-free nutrient solution to the matrix is (600-800) ml: (1000-1500) ml;
After the lavender stem in the step 2) is inserted into a substrate, pre-culturing the lavender stem in a culture room with the temperature of 20-23 ℃ and the illumination time of 14-16h per day and the illumination intensity of 2000-5000lx for 1-2 days, adjusting the temperature to 23-25 ℃, adjusting the illumination intensity to 6000-10000lx and adjusting the illumination time of 14-16h per day;
The concentration of CO 2 in the culture room is adjusted to 1000-2000 PPm, and after the air supply is stopped for 20-30 min every 15min in the illumination time, the air supply is performed for 15min again, and the steps are repeated; then, the air supply amount is gradually increased by 200 ml/min every two days until 1600-2000ml/min;
The seedlings cultured in the step 3) are opened to a culture box cover, the temperature of a culture chamber is 23-27 ℃, the illumination intensity of the culture chamber is 8000-12000lx, the illumination time of the culture chamber is 14-16h each day, the concentration of CO 2 in the culture chamber is 1000-2000 PPm, and transplanting and planting are carried out after 1-2 days of culture.
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| CN1586176A (en) * | 2004-10-11 | 2005-03-02 | 中国科学院新疆理化技术研究所 | Tissue culturing method for lavender |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
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| AUPQ659800A0 (en) * | 2000-03-31 | 2000-04-20 | International Flower Developments Pty Ltd | Genetic sequences and uses therefor |
| CN101248179A (en) * | 2005-08-25 | 2008-08-20 | 东洋纺织株式会社 | Plant producing hyaluronic acid |
| WO2014085626A1 (en) * | 2012-11-27 | 2014-06-05 | University Of Florida Research Foundation, Inc. | Light modulation of plants and plant parts |
| WO2019143296A1 (en) * | 2018-01-19 | 2019-07-25 | Temasek Life Sciences Laboratory Limited | Methods of regeneration and transformation of stevia plant and transgenic stevia plants having enhanced steviol glycosides content |
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|---|---|---|---|---|
| CN1586176A (en) * | 2004-10-11 | 2005-03-02 | 中国科学院新疆理化技术研究所 | Tissue culturing method for lavender |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
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| CN116982555A (en) | 2023-11-03 |
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