CN102823492A

CN102823492A - Method for quickly propagating lavenders

Info

Publication number: CN102823492A
Application number: CN2012102794251A
Authority: CN
Inventors: 杨波; 李洪林
Original assignee: Wuhan Botanical Garden of CAS
Current assignee: Wuhan Botanical Garden of CAS
Priority date: 2012-08-08
Filing date: 2012-08-08
Publication date: 2012-12-19
Anticipated expiration: 2032-08-08
Also published as: CN102823492B

Abstract

The invention discloses a method for quickly propagating lavenders, which comprises the steps: a, based on the lavender young sprout as an explant, cutting the lavender young sprout into stem sections with the lengths of 2-3 centimeters, disinfecting by using 0.1% mercuric chloride, flushing by using sterile water, cutting the young sprout sections into the sections with 1-2 buds, and grafting the sections in a prepared culture medium which comprises a Murashige and Skoog (MS) minimal medium and additional substances with different ingredients; and culturing under the conditions of the temperature of 20-28 DEG C, the illumination intensity of 3000-10000 luces, and illumination time of 12-16 hours every day for 5-7 days so that lateral bus grow with young sprouts; b, cutting the cultured young sprouts, grafting the young sprouts into the culture medium for subculture, and expanding the propagation for one time every 15-25 days; and c, cutting the young spouts which the lengths of 3-5 centimeters from the expanded propagating tissue culture seedlings, inserting the young spouts into a full exposure atomizing cutting propagation bed for direct rooting and seedling domestication, and realizing the rooting and transplanting after 30-40 days. The method is feasible, is convenient to operate, and has high propagating and rooting speeds, a strong root system and high survival rate.

Description

A kind of lavender is the method for breeding fast

Technical field

The invention belongs to plant seedling quick propagating technology field, more specifically relate to a kind of lavender method of breeding fast, be suitable for aromatic plant and breed fast.

Technical background

Lavandula Linn. Lavender belongs to, Labiatae.Fruticuli or undershrub, rare is draft.Leaf is linear to lanceolar or pinnation.Wheel umbrella inflorescence tool 2～10 flowers are gathered into to push up to give birth at Zhi Ding usually and are interrupted or nearly continuous spike.Spend blueness or purple, short stalk of tool or nearly nothing stalk.About 28 kinds, be distributed in Atlantic Ocean Islands and Mediterranean Region to Somalia, the false China of Pakistan and India only cultivates 2 kinds.

Containing aromatic oil in spending, is one of important in the world natural perfume plant.Because the essential oil that its flower fringe extracts has pure and fresh and fragrant pleasant fragrance, be widely used in the multiple daily cosmetics, be important material kind in the blending, also be one of important material of medicine and chemical industry; Lavender just is widely used in the medical treatment from ancient times, and stem and Ye Douke are used as medicine, have be good for the stomach, the effect of sweating, pain relieving, be the good medicine of treatment cold, stomachache, eczema; Graceful elegant because of its leaf pattern, bluish violet inflorescence grain husk is long beautiful, also is a kind of important ornamental plants.

Lavender originates in MediterraneanLittoral, EuropeVarious places reach OceaniaArchipelago, the back is extensively planted in Britain and is reached Yugoslavia, existing The U.S. The Tennessee State, Japan HokkaidoA large amount of plantations are also arranged.Xinjiang Yi Li introduced french lavender in 1964, because its superior climatic conditions and good quality, plantation training area enlarges rapidly.Her pears river valley regional culture lavender area reached 5400hm in 2010 ², account for about 90% of national lavender cultivated area, be maximum lavender planting base, the whole nation, be called three big lavender bases in the world with the Provence of France, the Fu Liangye of Hokkaido, Japan.Yi Li and French Provence natural perfume material are less than the territory, producing region and are in same latitude; Husky valley floor band is ringed on three sides by mountains; Belong to the semi-arid climate district; Following and the suitable lavender cultivation of water and soil photo-thermal resource of weather conditions can be produced the first-class fragrant plant resource of high-quality, and the Yi Li lavender becomes one of the world's eight big top kinds as famous and precious natural perfume material crop.

The breeding of lavender is main with sowing, cuttage and division propagation.But the seed propagation offspring separates, and can not keep the purity of improved seeds; The numerous survival rate of cuttage is low and need a large amount of female parents, can not satisfy the demand in market; And long-term nourishing and generating is prone to the deterioration of variety phenomenon.For this reason; Adopt plant tissue culture technique; The seedling of fine individual plant, strain and the kind of lavender is produced in batch production, one can overcome variet complexity that sowing and cuttage bring, plant problem such as sexual involution, and two can not receive the annual high quality seedling of growing of influence of physioclimate; Can also accelerate the update of lavender industry in a short time; The fast development of promotion industry also can also have great importance to the healthy and sustainable development that promotes China's fragrant plant industry for further breeding research provides material and technical support.

At present, the existing report of relevant lavender tissue culture has through stem apex, stem section to obtain regeneration plant; Chinese patent CN200410085334X tissue culturing method for lavender is to use the lavender blade to obtain regeneration plant for growing the body evoked callus outward and carrying out suspension culture; Chinese patent CN200610059350.0 is that explant breaks up the acquisition regeneration plant that sprouts with the lavender hypocotyl.

Summary of the invention

The objective of the invention is to be to provide a kind of lavender method of breeding fast, method is simple, easy to operate, and breeding, rooting rate are fast, and root system is healthy and strong, survival rate is high.Adopt this kind method can breed the lavender seedling that has stalwartness, flourishing root system in a large number fast, satisfied the demand of large-scale planting, for the foundation of large-scale production of lavender high quality seedling and genetic conversion system lays the foundation.

In order to achieve the above object, the present invention adopts following technical measures:

The present invention is an explant with the lavender young sprout, can carry out successive transfer culture after sprouting in 5-7 days, cultivates and just can directly take root and refine seedling in 15～25 days, and rooting rate can reach more than 98%.The present invention compares with other tissue culture method: have aspect induced bud and the successive transfer culture program simply, advantage fast; Aspect culture of rootage owing to take root and refine seedling and carry out synchronously, unite two into one two steps of seedling of taking root, refine of traditional tissue culture, shortened the production cycle, improved tissue cultivating seedling survival rate, reduced the production cost of tissue cultivating seedling.

A kind of lavender is the method for breeding fast, the steps include:

A, with the lavender young sprout as explant; Young sprout is cut into the long stem section of 2-3cm; Using concentration is 0.1% mercury chloride sterilization 4-8 minute, with aseptic water washing 3-4 time, again the young sprout section is cut into 1-2 bud one joint; Insert in the prepared culture medium, this medium comprises the material of MS minimal medium and additional different components; Placing temperature is 20-28 ℃, intensity of illumination 3000～10000Lx, and illumination every day 12-16 hour was cultivated 5-7 days, and its lateral bud grows young sprout, germination rate 80%;

B, cut the young sprout of cultivate sprouting, insert in the prepared culture medium and carry out successive transfer culture, expanded once numerous in every 15-25 days;

C, cut the long tender tip of 3-5cm and insert full exposure spraying slotting machine, directly take root and refine seedling, get final product rooting and transplant after 30～40 days from expanding numerous tissue cultivating seedling.This slotting machine is a matrix with sand or perlite.

The invention provides a kind of lavender seedling efficient system of breeding fast, compared with prior art, have the following advantages and effect; Easy to implement the method, easy to operate, reproduction speed is fast; 20 days propagation multiple reaches 4-5 doubly, and a bud can be bred 1,000,000 strain seedlings in 1 year.Culture of rootage is compared with general plant tissue culture technique: method is simple, unites two into one taking root and refining two steps of seedling, improved tissue culturing seedling transplanting survival rate, reduced the production cost of tissue culturing seedling.

Embodiment

Embodiment 1:

A kind of lavender is the method for breeding fast, the steps include:

1, with the lavender young sprout as explant, young sprout is cut into 3 or the long section of 4cm, using concentration is 0.1% mercury chloride sterilization 5 or 6 or 7 or 8 or 9 or 10 minutes, with aseptic water washing 3 or 4 times.

2, the young sprout section is cut into 1 or 2 buds, one joint, inserts in the prepared culture medium, this medium comprises the material of MS minimal medium and additional different components, and wherein additional composition is agar 6000-8000mg/L, and sugared 20000-30000mg/L is mixed.

3, illumination cultivation, temperature are 24 or 25 or 26 or 27 or 28 ℃, and light intensity is 3000 or 5000 or 8000 or 10000Lx, cultivate 5 or 6 or 7 days under the condition of illumination every day 12 or 13 or 14 or 15 or 16 hours, make lateral bud grow young sprout;

4, cut the young sprout of cultivating sprouting; Insert in the prepared culture medium and carry out successive transfer culture; This medium comprises the material of MS minimal medium and additional different components; Wherein additional composition is BA0.2-4mg/L, KT0.5-1.0mg/L, NAA0.01-0.4mg/L, IBA0.1-0.5mg/L, agar 6000-8000mg/L, and sugared 20000-30000mg/L is mixed; Expanded once numerous in per 15 or 18 or 22 or 25 days.

5, cut 3 or 4 or the long tender tip of 5cm from expanding numerous tissue cultivating seedling, cuttage is in the slotting machine of full exposure spraying, and this cutting bed is a matrix with river sand or perlite; Sprinkling irrigation concentration is 50 or 60 or 70 or 80 or 90 or the IBA of 100ppm or the root-inducing powder of 200ppm, but rooting and transplant after 30 or 34 or 37 or 40 days.

The composition of described MS minimal medium (unit: mg/L):

Additional composition:

Claims

1. the method that lavender breeds fast the steps include:

A. with the lavender young sprout as explant; Young sprout is cut into the long stem section of 2-3cm; Using concentration is 0.1% mercury chloride sterilization 4-8 minute, with aseptic water washing 3-4 time, again the young sprout section is cut into 1-2 bud one joint; Insert in the prepared culture medium, this medium comprises the material of MS minimal medium and additional different components; Placing temperature is 20-28 ℃, intensity of illumination 3000～10000Lx, and illumination every day 12-16 hour was cultivated 5-7 days, and its lateral bud grows young sprout;

B. cut and cultivate the young sprout of sprouting, insert in the prepared culture medium and carry out successive transfer culture, expanded once numerous in every 15-25 days;

C. cut the long tender tip of 3-5cm and insert full exposure spraying slotting machine from expanding numerous tissue cultivating seedling, directly take root and refine seedling, get final product rooting and transplant after 30～40 days;

The composition of described MS minimal medium, unit: mg/L:

Described additional composition: