CN102986528A - Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof - Google Patents
Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof Download PDFInfo
- Publication number
- CN102986528A CN102986528A CN2012104532893A CN201210453289A CN102986528A CN 102986528 A CN102986528 A CN 102986528A CN 2012104532893 A CN2012104532893 A CN 2012104532893A CN 201210453289 A CN201210453289 A CN 201210453289A CN 102986528 A CN102986528 A CN 102986528A
- Authority
- CN
- China
- Prior art keywords
- seedling
- rooting
- lavender
- leatherleaf
- enhancing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a seedling enhancing and rooting method of lavandula pinnata and a seedling enhancing and rooting culture medium of the lavandula pinnata, and belongs to the technical field of cultivating of plant tissues. The seedling enhancing and rooting culture medium of the lavandula pinnata comprises MS (Murashige and Skoog), 0.2 to 1mg/L of ancymidol, and 30g/L of cane sugar; and the pH (Potential of Hydrogen) is 5.8. By adopting the seedling enhancing and rooting method disclosed by the invention, the lavandula pinnata can be given more roots, the rooting can be realized quickly, and the rooted seedling is shortened, thus the seedling can be enhanced and easily survive after being transferred. By adopting the rooting culture medium to induce the lavandula pinnata, the lavandula pinnata can be given more roots, the rooting can be quickly realized, the seedling is strong, 10 +/- 2 roots are averagely provided for each plant, the plant is 4+1cm/50d in mean height, and the transplanting survival rate is up to 95 to 100%.
Description
Technical field
The invention belongs to field of plant tissue culture technique.Be specifically related to strengthening seedling and rooting method and the root media thereof of leatherleaf lavender.
Background technology
[0002] lavender is that the Labiatae lavender belongs to perennial undershrub, and strong aromatic odor is arranged, and originates in Mediterranean.The lavender genus (
Lavandula, L.), 32 initial specieses are arranged approximately, be distributed in Atlantic Ocean Islands and Mediterranean Region to Somalia, Pakistan and India; Only 2 kinds of the original cultivation of China, namely narrow leaf lavender (
Lavandula angustifolia, Mill) or true lavender, and wide leaf lavender (
L. spica), now again gradually from external introduced the leatherleaf lavender (
Lavendulapinnat), the tingia lavender (
L.dentata) etc. a plurality of kinds.Wherein, the leatherleaf lavender (
Lavendula pinna) be the longest initial species of florescence, especially in the Kunming that it's like spring all the year round, as long as pruning is proper, nutrition is abundant, can continue throughout the year to bloom.Because of its aromatic flavour, significant in perfumery etc. again.But, solid hardly after the leatherleaf lavender of introducing at present blooms, obtain difficulty of seed.Propagation method can only be by buying seed or utilizing vegetative propagation.
Isolated vegetative propagation is fast with its distinctive reproduction speed, the cycle is short, it is little to take up room, and the characteristics such as can also carry out in the anniversary are the good methods of micropropagation of plants always.Although existing people has reported the quick-breeding method of lavender, but in our conscientiously fast numerous process, find, perhaps be the characteristics of leatherleaf lavender self, so that the numerous seedling that goes out of conventional fast numerous rooting method is very thin, thin and weak, more crucial is, when adding the auxins plant growth regulator (NAA0.2-1mg/L) of common hestening rooting in the MS medium, leatherleaf lavender group training seedling can not be taken root, and is to grow a lot of callus (Fig. 1 and Fig. 2) at root on the contrary.
Summary of the invention
The technical problem to be solved in the present invention is to overcome defective and the deficiency that prior art exists the group training seedling of culture of rootage very thin, thin and weak, can not take root during the tissue of leatherleaf lavender is cultivated, and its objective is that providing a kind of can make leatherleaf lavender plant downgrade seedling strengthen, take root many, take root strengthening seedling and rooting method and root media thereof fast, that transplanting easily survives.
For solving the problems of the technologies described above, technical scheme of the present invention is as follows:
1, a kind of leatherleaf lavender (
Lavendula pinnat) the strengthening seedling and rooting medium be: MS+ ancymidol 0.2～1mg/L+ sucrose 30g/L, pH=5.8.
2, a kind of leatherleaf lavender (
Lavendula pinnat) the strengthening seedling and rooting method, the method is to be inoculated in root induction in the strengthening seedling and rooting medium under aseptic condition by the leatherleaf lavender indefinite bud of existing method for plant tissue culture cultivation or the leatherleaf lavender indefinite bud after the propagation, condition of culture: temperature is 23 ℃ ± 2 ℃, intensity of illumination is 2500 lx, be 12 h/12 h illumination/interlunation, used strengthening seedling and rooting medium is: MS+ ancymidol 0.2～1mg/L+ sucrose 30g/L, pH=5.8.
The preferred strengthening seedling and rooting medium of above-mentioned two technical schemes is: MS+ ancymidol 0.5mg/L+ sucrose 30g/L, pH=5.8.
Compared with prior art, the invention has the beneficial effects as follows:
Prior art is when culture of rootage, often being added in auxins plant growth regulator and the concentration thereof of adding hestening rooting in the MS medium is: NAA0.2～1mg/L, yet, the inventor studies and finds that the leatherleaf lavender is in the MS medium of the auxins plant growth regulator NAA0.2～1mg/L that is added with hestening rooting, its group training seedling grows a lot of callus at root, seedling is very thin, thin and weak, (see Fig. 1, Fig. 2, table 1), therefore, it is inconsistent that the contained endogenous plant hormone of prediction leatherleaf lavender is cultivated thing with conventional group, and this may be that the characteristics of leatherleaf lavender self cause.And be in the MS medium, to have added pyrimidine 0.2～1mg/L at medium of the present invention, can make the leatherleaf lavender take root many, take root fast, can also make the seedling of taking root downgrade so that seedling strong, thereby transplant and also easily survive.Can make the average every strain of leatherleaf lavender take root 10 ± 2 with medium of the present invention and cultural method, group training seedling plant height average out to 4cm
+1cm/ 50d, plant become blackish green, transplant easily to survive, and transplanting survival rate is up to 95～100%.(Fig. 3, Fig. 4, Fig. 5, table 2), and adopt prior art root media (MS+ NAA0.2-1mg/L+ sucrose 30g/L) root induction commonly used, but can not take root, compared with prior art, the present invention has produced unforeseeable technique effect.
Description of drawings
Fig. 1 is to be MS+ NAA0.2mg/L+ sucrose 30g/L at the check experiment medium, the performance of taking root of leatherleaf lavender among the pH=5.8, and the leatherleaf lavender can not take root in this medium, and long callus.
Fig. 2 is to be MS+NAA0.5～1mg/L+ sucrose 30g/L at the check experiment medium, the performance of taking root of leatherleaf lavender among the pH=5.8.The leatherleaf lavender can not take root and long callus in this medium.
Fig. 3 is at medium MS+ ancymidol 0.5mg/L+ sucrose 30g/L of the present invention, the leatherleaf lavender performance of taking root among the pH=5.8.
Fig. 4 is at medium MS+ ancymidol 1mg/L+ sucrose 30g/L of the present invention, the leatherleaf lavender performance of taking root among the pH=5.8.
Fig. 5 is at medium MS+ ancymidol 0.2mg/L+ sucrose 30g/L of the present invention, the leatherleaf lavender performance of taking root among the pH=5.8.
Embodiment
Below each embodiment with the leatherleaf lavender (
Lavendula pinnat) young tender brachyplast be explant, it is pure that the test materials such as each reagent of medium are commercially available analysis, ancymidol (Sigma company).The leatherleaf lavender (
Lavendula pinnat) also can buy from market.Below each embodiment be if no special instructions conventional method.Each embodiment adopts the method for plant tissue culture of prior art to carry out to the propagation of the young tender brachyplast sterilization of leatherleaf lavender, indefinite spore induction, indefinite bud, repeats no more.
Embodiment 1 medium is: MS+ ancymidol 0.2 mg/L+ sucrose 30g/L, pH=5.8.
The leatherleaf lavender that method for plant tissue culture is routinely cultivated (
Lavendula pinnat) indefinite bud, under aseptic condition, be inoculated in the strengthening seedling and rooting medium: MS+ ancymidol 0.2 mg/L+ sucrose 30g/L, root induction among the pH=5.8, condition of culture: temperature is 23 ℃ ± 2 ℃, intensity of illumination 2500 lx, be 12 h/12 h illumination/interlunation.Take root number, root is long and plant height sees Table 1.
Embodiment 2 medium are: MS+ ancymidol 0.5mg/L+ sucrose 30g/L, pH=5.8.
The leatherleaf lavender that method for plant tissue culture is routinely cultivated (
Lavendula pinnat) indefinite bud, under aseptic condition, be inoculated in the strengthening seedling and rooting medium: MS+ ancymidol 0.5 mg/L+ sucrose 30g/L, root induction among the pH=5.8, condition of culture is identical with embodiment 1, and the number of taking root, root length and plant height see Table 1.
Embodiment 3 medium are: MS+ ancymidol 1mg/L+ sucrose 30g/L, pH=5.8.
The leatherleaf lavender that method for plant tissue culture is routinely cultivated (
Lavendula pinnat) indefinite bud, under aseptic condition, be inoculated in the strengthening seedling and rooting medium: MS+ ancymidol 1 mg/L+ sucrose 30g/L, root induction among the pH=5.8, condition of culture is identical with embodiment 1, and the number of taking root, root length and plant height see Table 1.
Fig. 3-Fig. 5 shows that the leatherleaf lavender takes root many in medium of the present invention.The leatherleaf lavender takes root quantity than embodiment 1-2 raising 13%～66.7% in MS+ ancymidol 0.5mg/L+ sucrose 30g/L medium, and root system is also flourishing especially, compares with embodiment 1-2, and its effect is more excellent, has produced unforeseeable technique effect.
Embodiment 4 check experiments, medium is: MS+NAA0.2～1mg/L+ sucrose 30g/L, pH=5.8.
The leatherleaf lavender that method for plant tissue culture is routinely cultivated (
Lavendula pinnat) indefinite bud, under aseptic condition, be inoculated in respectively 1. MS+ NAA 0.2 mg/L+ sucrose 30g/L of root media, pH=5.8; 2. MS+ NAA 0.5 mg/L+ sucrose 30g/L, pH=5.8; 3. MS+ NAA 1 mg/L+ sucrose 30g/L, pH=5.8; Condition of culture when adopting this three medium culture is all identical with embodiment 1.The number of taking root during three medium culture, root length and plant height see Table 1, and Fig. 1 and Fig. 2 are seen in the growth performance, and Fig. 1 and Fig. 2 show: the leatherleaf lavender can not take root in the described medium of contrast, and long callus.
More than each embodiment hardening and transplanting method by the Plant Tissue Breeding routine after culture of rootage carry out hardening and transplanting, repeat no more, its transplant survival effect sees Table 1.
Train the seedling rooting situation as can be known from the leatherleaf lavender group of above embodiment: 1. the MS medium, if add NAA(concentration: 0.2-1mg/L) then stimulate the cell division of leatherleaf lavender vigorous, to such an extent as to can not build up root morphology, but formation callus, and can promote that plant grows tall fast, so that seedling is very thin, excessive growth (plant height can arrive tens centimetres).If 2. in the MS medium, add ancymidol (concentration: 0.2-1 mg/L) then can induce leatherleaf lavender plant to take root smoothly, and take root many, plant is short strong, seedling is blackish green.But along with ancymidol concentration raises, leatherleaf lavender plant strain growth is subject to suppressing more by force, though plant is still aobvious blackish green, and plant height less (2-4 centimetre).3. this experimental study is found: ancymidol can be used as the growth inhibitor in the group training experiment, can urge the indefinite bud strengthening seedling and rooting.Therefore, this experimental study has gone out the culture medium prescription that the leatherleaf lavender takes root and Reducing sugar is splendid and has been: MS+ ancymidol 0.5 mg/L+ sucrose 30g/L, pH=5.8.
Claims (4)
- A leatherleaf lavender ( Lavendula pinnat) the strengthening seedling and rooting medium be: MS+ ancymidol 0.2～1mg/L+ sucrose 30g/L, pH=5.8.
- Leatherleaf lavender according to claim 1 ( Lavendula pinnat) the strengthening seedling and rooting medium, it is characterized in that: described leatherleaf lavender ( Lavendula pinnat) the strengthening seedling and rooting medium be MS+ ancymidol 0.5mg/L+ sucrose 30g/L, pH=5.8.
- A leatherleaf lavender ( Lavendula pinnat) the strengthening seedling and rooting method, it is characterized in that: the method is to be inoculated in root induction in the strengthening seedling and rooting medium under aseptic condition by the leatherleaf lavender indefinite bud of existing method for plant tissue culture cultivation or the leatherleaf lavender indefinite bud after the propagation, condition of culture: temperature is 23 ℃ ± 2 ℃, intensity of illumination is 2500 lx, be 12 h/12 h illumination/interlunation, used strengthening seedling and rooting medium is: MS+ ancymidol 0.2～1mg/L+ sucrose 30g/L, pH=5.8.
- Leatherleaf lavender according to claim 3 ( Lavendula pinnat) the strengthening seedling and rooting method, it is characterized in that: described strengthening seedling and rooting medium is: MS+ ancymidol 0.5mg/L+ sucrose 30g/L, pH=5.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104532893A CN102986528A (en) | 2012-11-13 | 2012-11-13 | Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104532893A CN102986528A (en) | 2012-11-13 | 2012-11-13 | Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102986528A true CN102986528A (en) | 2013-03-27 |
Family
ID=47916017
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012104532893A Pending CN102986528A (en) | 2012-11-13 | 2012-11-13 | Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102986528A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106942053A (en) * | 2017-03-10 | 2017-07-14 | 江西省农业科学院农产品质量安全与标准研究所 | A kind of tissue culture and rapid propagation method for Xingan lucid asparagus |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0328424A2 (en) * | 1988-02-12 | 1989-08-16 | Kyowa Hakko Kogyo Kabushiki Kaisha | Process for the production of somatic embryos |
| JPH0724519B2 (en) * | 1989-06-30 | 1995-03-22 | 全国農業協同組合連合会 | Potato tuber manufacturing method |
| JPH089811A (en) * | 1994-07-04 | 1996-01-16 | Nursery Technol:Kk | Storage of young rice plant and raising seedling of stored young rice plant |
| CN1586176A (en) * | 2004-10-11 | 2005-03-02 | 中国科学院新疆理化技术研究所 | Tissue culturing method for lavender |
| CN1817112A (en) * | 2006-03-03 | 2006-08-16 | 中国科学院新疆理化技术研究所 | Fast lavandulol regeneration |
| CN101843220A (en) * | 2010-05-12 | 2010-09-29 | 北京先农科芦笋研发中心 | Asparagus parent breeding method |
| ES2349102B1 (en) * | 2008-12-15 | 2012-04-20 | Consejo Superior de Investigaciones CientÃficas (CSIC) | PROCEDURE FOR IN VITRO PROPAGATION OF ASPARAGUS |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
-
2012
- 2012-11-13 CN CN2012104532893A patent/CN102986528A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0328424A2 (en) * | 1988-02-12 | 1989-08-16 | Kyowa Hakko Kogyo Kabushiki Kaisha | Process for the production of somatic embryos |
| JPH0724519B2 (en) * | 1989-06-30 | 1995-03-22 | 全国農業協同組合連合会 | Potato tuber manufacturing method |
| JPH089811A (en) * | 1994-07-04 | 1996-01-16 | Nursery Technol:Kk | Storage of young rice plant and raising seedling of stored young rice plant |
| CN1586176A (en) * | 2004-10-11 | 2005-03-02 | 中国科学院新疆理化技术研究所 | Tissue culturing method for lavender |
| CN1817112A (en) * | 2006-03-03 | 2006-08-16 | 中国科学院新疆理化技术研究所 | Fast lavandulol regeneration |
| ES2349102B1 (en) * | 2008-12-15 | 2012-04-20 | Consejo Superior de Investigaciones CientÃficas (CSIC) | PROCEDURE FOR IN VITRO PROPAGATION OF ASPARAGUS |
| CN101843220A (en) * | 2010-05-12 | 2010-09-29 | 北京先农科芦笋研发中心 | Asparagus parent breeding method |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
Non-Patent Citations (4)
| Title |
|---|
| 《岡山大農学報》 19911231 松原幸子等 "アスパラガス側芽培養でのアンシミドールによる発根及び多芽体形成" 第9-15页 , 第77期 * |
| 《神大農研報（Sci. Rept. Fac. Agr. Kobe Univ.）》 19961231 Noboru Inagaki等 "On the optimal conditions for Organogenesis through anther-derived calli of Asparagus" 第19-24页 第22卷, * |
| NOBORU INAGAKI等: ""On the optimal conditions for Organogenesis through anther-derived calli of Asparagus"", 《神大農研報（SCI. REPT. FAC. AGR. KOBE UNIV.）》, vol. 22, 31 December 1996 (1996-12-31), pages 19 - 24 * |
| 松原幸子等: ""アスパラガス側芽培養でのアンシミドールによる発根及び多芽体形成"", 《岡山大農学報》, no. 77, 31 December 1991 (1991-12-31), pages 9 - 15 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106942053A (en) * | 2017-03-10 | 2017-07-14 | 江西省农业科学院农产品质量安全与标准研究所 | A kind of tissue culture and rapid propagation method for Xingan lucid asparagus |
| CN106942053B (en) * | 2017-03-10 | 2019-04-09 | 江西省农业科学院蔬菜花卉研究所 | A kind of tissue culture and rapid propagation method for Xingan lucid asparagus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ari et al. | Androgenic responses of 64 ornamental pepper (Capsicum annuum L.) genotypes to shed-microspore culture in the autumn season | |
| Yıldırım | Micropropagation of Pistacia lentiscus L. from axenic seedling-derived explants | |
| CN102577969B (en) | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 | |
| CN108901856B (en) | method for efficient somatic embryogenesis and plant regeneration of camellia plants | |
| CN104099287A (en) | Acquiring and subculture maintaining method for high yield oligomeric proanthocyanidins vitis davidii callus | |
| CN107864862B (en) | Method for quickly and efficiently obtaining tissue culture seedlings of ceiba | |
| CN108029559B (en) | Method for quickly cultivating ilex latifolia tissue culture seedlings | |
| Lesar et al. | Acclimatization of terrestrial orchid Bletilla striata Rchb. f.(Orchidaceae) propagated under in vitro conditions | |
| CN102870604A (en) | Aseptic grafting method solving difficulty of soybean transformed adventitious buds in rooting | |
| CN102187812A (en) | Method for establishing efficient plant regeneration system by using hevea brasiliensis embryonic cell suspension system | |
| Michael | Effects of coconut water on callus initiation and plant regeneration potentials of sweetpotato | |
| CN108142283B (en) | Tissue culture rapid propagation method of Acer catalpa Maxim | |
| CN112715367B (en) | Method for carrying out Maozu secondary proliferation by using lanthanum nitrate | |
| CN114027182A (en) | Tissue culture propagation method for dolichos succulent plants in crassulaceae echeveria | |
| CN110476811B (en) | Method for improving quality of double-line arrowroot tissue culture seedlings | |
| Rangsayatorn | Micropropagation of Dendrobium draconis Rchb. f. from thin cross-section culture | |
| CN102577972A (en) | Method for tissue culture of hoya kerrii | |
| CN109122315B (en) | Method for cultivating seedlings by using alum root and petioles | |
| CN107593448B (en) | Method for in-vitro rapid propagation by utilizing aseptic seedling stem segments of sedum aizoon | |
| CN102577970A (en) | Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts | |
| CN102986528A (en) | Seedling enhancing and rooting method of lavandula pinnata and seedling enhancing and rooting culture medium thereof | |
| CN111280054A (en) | Hydrangea paniculata tissue culture proliferation and rooting integrated seedling method and used culture medium | |
| KR101681062B1 (en) | Optimal medium composition for tissue culture of Chrysanthemum morifolium 'Baekma' and tissue culture method using the same | |
| CN104381132B (en) | Rooting method of Juglans hindsii*J.regia vlach tissue culture seedling in bottle | |
| Nazneen et al. | MICRO) PROPA (GATION (OF P () LIANTHUS TUBER () S4 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130327 |