JPS6291122A - Method for growing seedling of lavender - Google Patents
Method for growing seedling of lavenderInfo
- Publication number
- JPS6291122A JPS6291122A JP60233796A JP23379685A JPS6291122A JP S6291122 A JPS6291122 A JP S6291122A JP 60233796 A JP60233796 A JP 60233796A JP 23379685 A JP23379685 A JP 23379685A JP S6291122 A JPS6291122 A JP S6291122A
- Authority
- JP
- Japan
- Prior art keywords
- callus
- lavender
- medium
- plants
- seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明はラベンダー属植物の育苗方法、特に組織培養に
よりラベンダー属植物の幼苗を大量に得る方法に関する
。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a method for raising seedlings of plants of the genus Lavender, particularly to a method for obtaining large quantities of young seedlings of plants of the genus Lavender by tissue culture.
（従来の技術）
ラベンダー（Ｌａｖａｎｄｕｌａ ｏｆｆｉｃｉｎａｌ
ｉｓ）などのラベンダー属植物は古くから香料植物とし
て知られ。(Prior art) Lavender (Lavandula official)
Lavender plants such as is) have been known as aromatic plants since ancient times.
その花から採取された酢酸リナロール（ｌｉｎａｌｌｙ
ｌａｃｅ ｔａ ｔｅ）を主成分とするラベンダー油は
矯味料。Linalool acetate (linally) collected from its flowers
Lavender oil, whose main ingredient is lace tate, is a flavoring agent.
香水２石鹸などの香料に利用されている。ラベンダー属
植物の花は、鎮痛、鎮痙作用を有する生薬としても知ら
れ、胃、呼吸器、胆道疾患には内服薬として、リウマチ
、神経痛、痛風には外用薬として利用されている。その
ため、ラベンダー属植物からラベンダー油や上記生薬を
工業的規模で生産することが望まれている。しかし、ラ
ベンダー属植物は増殖率が極めて低い。通常、その種子
からは発芽させることが難しいため、挿し木などの方法
を用いて幼苗を得ている。幼苗からの生育も比較的遅い
。そのため、上記ラベンダー油などを大量に生産するこ
とが難しい。ラベンダー属植物の幼苗を大量に得ること
ができれば、大量に栽培を行い、工業規模での生産が可
能になると考えられる。現在、洋間栽培などで植物の組
織培養により大量に幼苗を得ることが行われているが、
ラベンダー属植物からこのような方法で幼苗を得ること
は全く知られていない。Perfume 2 Used in fragrances such as soap. Flowers of plants of the genus lavender are also known as herbal medicines that have analgesic and antispasmodic effects, and are used as an internal medicine for gastric, respiratory, and biliary tract diseases, and as an external medicine for rheumatism, neuralgia, and gout. Therefore, it is desired to produce lavender oil and the above-mentioned crude drugs on an industrial scale from plants of the genus Lavender. However, plants of the genus Lavender have an extremely low proliferation rate. Normally, it is difficult to germinate seeds, so seedlings are obtained using methods such as cuttings. Growth from young seedlings is also relatively slow. Therefore, it is difficult to produce the above-mentioned lavender oil in large quantities. If large quantities of young seedlings of plants belonging to the genus lavender can be obtained, it would be possible to cultivate them in large quantities and produce them on an industrial scale. Currently, large quantities of young seedlings are obtained through plant tissue culture in Western cultivation, etc.
It is completely unknown to obtain seedlings from plants of the genus Lavender by this method.
（発明が解決しようとする問題点） 本発明は上記従来の欠点を解決するものであり。(Problem that the invention attempts to solve) The present invention solves the above-mentioned conventional drawbacks.
その目的とするところは、ラベンダー属植物の幼苗を短
期間で大量に得る方法を提供することにある。本発明の
他の目的は組織培養により、優れた形質を有するラベン
ダー属植物の幼苗を効果的に得る方法を提供することに
ある。The purpose is to provide a method for obtaining a large amount of seedlings of plants of the genus Lavender in a short period of time. Another object of the present invention is to provide a method for effectively obtaining seedlings of plants of the genus Lavender having excellent traits by tissue culture.
（問題点を解決するための手段）
本発明のラベンダー属植物の育苗方法は、ラベンダー属
植物の組織をオーキシン類およびサイトカイニン類を含
有するカルスＫＭ ｍ用培地を用いて培養しカルスを’
８ｆｓ ４する工程、該カルスをカルス増殖用培地で増
殖させる工程、および該カルスを再分化用培地で光を照
射して分化させ幼苗とする工程を包含し、そのことによ
り上記目的が達成される。(Means for Solving the Problems) The method for raising seedlings of plants of the genus Lavender of the present invention involves culturing tissues of plants of the genus Lavender using a medium for callus KMm containing auxins and cytokinins to grow callus.
8 fs 4, a step of growing the callus in a callus propagation medium, and a step of irradiating the callus with light in a redifferentiation medium to differentiate it into seedlings, thereby achieving the above purpose. .
本発明方法によれば、まず、ラベンダー属植物の根、茎
１葉などの化３■織の一部を切りとり、これを用いてカ
ルス誘導用培地で培養しカルスの誘導を行う。カルス誘
導用培地としては、植物組織培養に通常用いられるムラ
シゲ−スクーグの培地。According to the method of the present invention, first, a part of the keratinium, such as a root or a single leaf of a stem, of a plant of the genus Lavender is cut off, and this is used to culture in a callus induction medium to induce callus. The callus induction medium is Murashige-Skoog's medium, which is commonly used for plant tissue culture.
ホワイトの培地、リンスマイヤー−スクーグの培地、ガ
ウスレットの培地、ヘラ−の培地、およびこれらの改変
培地などが用いられ、これにカルスの誘導を促進させる
ためにオーキシン類およびサイトカイニン類が添加され
ている。オーキシン類には例えば、２・４−ジクロロフ
ェノキシ酢酸（２・４Ｄ）、インドール酢酸（［ＡＡ）
、ナフタレン酢酸（Ｎ Ａ Ａ）がある。サイトカイニ
ン類には１例えば、カイネチン、ヘンシルアデニンがあ
る。White's medium, Linsmeyer-Skoog's medium, Gauslett's medium, Heller's medium, and modified media thereof are used, and auxins and cytokinins are added to these to promote callus induction. There is. Examples of auxins include 2,4-dichlorophenoxyacetic acid (2,4D), indoleacetic acid ([AA)]
, naphthaleneacetic acid (NAA). Examples of cytokinins include kinetin and hensyl adenine.
・オーキシン類は通常、 ０．０１〜１０ｐｐｍの割合
で。・Auxins are usually contained at a rate of 0.01 to 10 ppm.
サイトカイニン類は０．０１〜１０ｐｐｍのＪ１１合で
培地中に含有される。これら植物ホルモン（オーキシン
類やサイトカイニン類）の量が過少であるとカルスが誘
導されに＜＜、過剰であると直接、不定根が分化したり
、生長阻害等が認められる。カルス誘導培地で１５〜３
５°Ｃにて１通常暗所で、培養を行うと２０〜５０日後
には組織切断面にカルスが形成される。必要に応じてこ
のカルスを適当な固体培地に移して適当な量にまで増殖
させる。Cytokinins are contained in the medium at a concentration of 0.01 to 10 ppm. If the amount of these plant hormones (auxins and cytokinins) is too low, callus will be induced. 15-3 in callus induction medium
When cultured at 5°C, usually in the dark, a callus is formed on the cut surface of the tissue after 20 to 50 days. If necessary, this callus is transferred to an appropriate solid medium and grown to an appropriate amount.
カルスを次に、カルス増殖用培地に移植して培養を行う
。カルス増殖用培地としてはムラシゲ−スクーグの培地
など組織培養に通常用いられる培地（寒天培地などの固
体培地または液体培地）が利用されうる。上記カルス誘
導用培地と同様の培地を用いてもよい。オーキシン類の
濃度は１０ｐｐｍ以下とすることが好ましい。オーキシ
ン類の濃度が高すぎると安定したカルスの増殖が得られ
ない。The callus is then transplanted into a callus growth medium and cultured. As the callus growth medium, a medium (a solid medium such as an agar medium or a liquid medium) commonly used for tissue culture, such as Murashige-Skoog's medium, can be used. A medium similar to the above callus induction medium may be used. The concentration of auxins is preferably 10 ppm or less. If the concentration of auxins is too high, stable callus growth cannot be obtained.
植物ホルモンが全く含有されていなくてもよい。It may not contain any plant hormones at all.
培養は１５〜２５℃で通常暗所にて行われる。増殖した
カルスのうち増殖の速いカルスを選抜すれば。Cultivation is carried out at 15-25°C, usually in the dark. If callus that proliferates quickly is selected from among the proliferated calli.
生長の速いラベンダー属植物の幼苗が得られる。Young seedlings of fast-growing lavender plants can be obtained.
このようにして増殖したカルスを再分化用培地に移して
カルスを再分化させる。再分化用培地としては、植物組
織培養に通常用いられる培地にカイネチン、ベンジルア
デニンなどのサイトカイニン類が添加された。もしくは
無添加の９通常の植物カルス再分化用培地（固体培地）
が利用されうる。再分化用培地にオーキシン類が含有さ
れる場合には、その濃度はカルス増殖用培地よりも低（
設定する。オーキシン類が全く含有されていなくてもよ
い。このような再分化用培地にカルスを置床し、１０〜
２５℃、好ましくは１５〜２０°Ｃの温度条件下で、
１０００〜１００００ルクスの光を照射して培養を行う
。光量が過少であると再分化が行われず、過剰であると
生長阻害が認められる。このような条件下で培養を行う
と出芽・発根し、約２〜４ケ月でラベンダー属植物の幼
苗が得られる。The calli grown in this way are transferred to a regeneration medium to regenerate the callus. As the redifferentiation medium, cytokinins such as kinetin and benzyladenine were added to a medium commonly used for plant tissue culture. Or 9 ordinary plant callus regeneration medium (solid medium) without additives
can be used. When auxins are contained in the regeneration medium, their concentration is lower than that in the callus growth medium (
Set. It is not necessary to contain any auxins at all. Callus was placed on such a regeneration medium and incubated for 10 to 30 minutes.
Under a temperature condition of 25°C, preferably 15-20°C,
Culture is performed by irradiating light of 1,000 to 10,000 lux. If the amount of light is too low, redifferentiation will not occur, and if it is too much, growth will be inhibited. When cultured under such conditions, budding and rooting will occur, and seedlings of plants of the genus Lavender will be obtained in about 2 to 4 months.
（作用）
本発明方法では、植物ホルモンを含有する培地でラベン
ダー属植物のカルスが有利にＦ！される。(Effect) In the method of the present invention, the callus of plants of the genus Lavender is advantageously F! be done.
ラベンダー属植物の生長は比較的遅いにもかかわらず、
このカルスの形態においては速やかに増殖しうる。増殖
したカルスを再分化させると出芽・発根し幼苗が得られ
る。このように、短期間で大量のラベンダー属植物の均
質な幼苗が得られる。Although lavender plants grow relatively slowly,
This callus form can rapidly proliferate. When the proliferated callus is redifferentiated, it will sprout and root, producing young seedlings. In this way, a large amount of homogeneous seedlings of lavender plants can be obtained in a short period of time.
カルス増殖時に増殖の速いカルスを選抜すれば。If calli that proliferates quickly are selected during callus proliferation.
生長の速いラベンダー属植物の幼苗が得られる。Young seedlings of fast-growing lavender plants can be obtained.
（実施例） 以下に本発明を実施例について述べる。(Example) The present invention will be described below with reference to examples.
大施炎工
（Ａ）カルスの誘導ニラベンダー（Ｌａｖａｎｄｕｌａ
ｏｆｆｉｃｉｎａｌｉｓ）の葉を適当な大きさに切りと
り、これを７０％エタノール水溶液に３０秒間浸漬し、
さらに次亜塩素酸ナトリウム水溶液（ＣＩ：ａ度１．５
％）に８分間浸漬して殺菌処理を行った。これを無菌水
で洗浄ａＯ，Ｓ〜２．ＯｃＪの大きさに切断し、カルス
誘導用培地に置床した。カルス誘導用培地としてはオー
キシン類として２・４Ｄを１ ｐｐｍの割合で、サイト
カイニン類としてカイネチンを０．ｌｐｐｍの割合で含
有するムラシゲ−スクーグの寒天培地を用いた。２５℃
で暗所にて２０日間培養を行ったところ切口にカルスが
形成された。Large flame construction (A) Induction of callus Lavandula
Cut the leaves of S. officinalis to an appropriate size and soak them in a 70% ethanol aqueous solution for 30 seconds.
Furthermore, sodium hypochlorite aqueous solution (CI: a degree 1.5
%) for 8 minutes for sterilization. Wash this with sterile water aO,S~2. It was cut to the size of OcJ and placed on a callus induction medium. The callus induction medium contains 1 ppm of 2.4D as an auxin and 0.0 ppm of kinetin as a cytokinin. A Murashige-Skoog agar medium containing 1ppm was used. 25℃
When cultured in the dark for 20 days, callus was formed at the cut end.
（Ｂ）カルスの増殖： （Ａ）項で形成されたカルスを
カルス増殖用培地に移植し、２５℃で２週間培養を行っ
た。カルス増殖用培地としては、オーキシン類としてＮ
ＡＡを１０ｐｐｍの割合で、そしてサイトカイニン類と
してベンジルアデニンを０．５ｐｐｍの割合で含有する
ムラシゲ−スクーグの寒天培地を用いた。(B) Proliferation of callus: The callus formed in section (A) was transplanted to a callus growth medium and cultured at 25°C for 2 weeks. As a callus growth medium, N is used as an auxin.
A Murashige-Skoog agar medium containing AA at a rate of 10 ppm and benzyladenine as a cytokinin at a rate of 0.5 ppm was used.
（Ｃ）カルスの再分化：サイトカイニン類としてカイネ
チンを１．０ｐｐｍの割合で含有し、オーキシン類を含
有しないムラシゲ−スクーグの寒天培地を調製し再分化
用培地とした。（Ｂ）項で得られた増殖カルスをこの再
分化用培地に置床し。(C) Regeneration of callus: A Murashige-Skoog agar medium containing 1.0 ppm of kinetin as a cytokinin and no auxins was prepared and used as a regeneration medium. The proliferated callus obtained in section (B) was placed on this regeneration medium.
２０００ルクスの光をあてて２５℃にて培養を行った。Culture was performed at 25°C under 2000 lux light.
置床後約３０日間で出芽・発根がはじまり約４ケ月後に
は２〜４ｃ＋ｍに成長した多数の幼苗が得られた。Approximately 30 days after placing the seedlings, germination and rooting began, and approximately 4 months later, many seedlings that had grown to 2 to 4 cm were obtained.
このように、ラベンダーを種子から発芽させる方法や挿
し木による増殖法に比べて極めて短期間で幼苗が得られ
る。In this way, seedlings can be obtained in an extremely short period of time compared to the method of germinating lavender from seeds or the method of propagating lavender using cuttings.
失施拠１
（Ａ）カルスの誘導：実施例１ （Ａ）項と同様である
。Failure 1 (A) Induction of callus: Same as Example 1 (A).
（Ｂ）カルスの増殖ニオ−キシン類としてＮＡＡをｉ
ｐｐｍの割合で、そしてサイトカイニン類としてカイネ
チンを１０ｐｐｎ＋の割合で含有するムラシゲ−スクー
グの液体培地をカルス増殖用培地としたこと以外は実施
例１ （Ｂ）項と同様である。(B) Use of NAA as a nioxin for callus growth
The procedure was the same as in Example 1 (B) except that a Murashige-Skoog liquid medium containing kinetin as a cytokinin at a ratio of 10 ppm and 10 ppn+ was used as the medium for callus growth.
（Ｃ）本実施例（Ｂ）項で得られた増殖カルスを用い、
実施例１ （Ｃ）項に準じてカルスを再分化させた。置
床後約３０日間で出芽・発根がはじまり約２ケ月後には
２〜４ｃｍに成長した多数の幼苗が得られた。(C) Using the proliferated callus obtained in section (B) of this example,
Example 1 Callus was redifferentiated according to Section (C). Approximately 30 days after placement, germination and rooting began, and approximately 2 months later, many seedlings that had grown to 2 to 4 cm were obtained.
（発明の効果）
本発明によれば、このように９組織培養法により短期間
で大量のラベンダー属植物の均質な幼苗が得られる。組
織培養法を用いるので、地質や気候などの自然条件を考
慮することなく、かつ広い土地を必要とせずに幼苗の育
成が可能である。カルス培養時に増殖の速いカルスを選
択すれば、生長の速いラベンダー属植物の幼苗が得られ
る。大量に得られたラベンダー属植物を栽培すれば、ラ
ベンダー油やラベンダー生薬が大量かつ安価に提供され
うる。(Effects of the Invention) According to the present invention, a large amount of homogeneous young seedlings of plants belonging to the genus Lavender can be obtained in a short period of time using the nine-tissue culture method. Since the tissue culture method is used, it is possible to grow seedlings without considering natural conditions such as geology or climate, and without requiring a large area of land. If callus that proliferates quickly is selected during callus culture, young seedlings of lavender plants that grow quickly can be obtained. If large amounts of lavender plants are cultivated, lavender oil and lavender herbal medicines can be provided in large quantities and at low cost.
Claims (1)
トカイニン類を含有するカルス誘導用培地を用いて培養
しカルスを誘導する工程、 該カルスをカルス増殖用培地で増殖させる工程、および 該カルスを再分化用培地で光を照射して分化させ幼苗と
する工程、 を包含するラベンダー属植物の育苗方法。 ２、前記再分化用培地のオーキシン類の濃度が前記カル
ス増殖用培地のオーキシン類の濃度以下である特許請求
の範囲第１項に記載の方法。 ３、前記照射光の照度が１０００〜１００００ルクスで
ある特許請求の範囲第１項に記載の方法。[Scope of Claims] 1. A step of inducing callus by culturing tissues of plants of the genus Lavender using a callus induction medium containing auxins and cytokinins, a step of growing the callus in a callus growth medium, and A method for raising seedlings of plants of the genus Lavender, comprising the step of irradiating the callus with light in a redifferentiation medium to differentiate the callus into seedlings. 2. The method according to claim 1, wherein the concentration of auxins in the regeneration medium is lower than the concentration of auxins in the callus growth medium. 3. The method according to claim 1, wherein the illuminance of the irradiation light is 1,000 to 10,000 lux.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60233796A JPS6291122A (en) | 1985-10-18 | 1985-10-18 | Method for growing seedling of lavender |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60233796A JPS6291122A (en) | 1985-10-18 | 1985-10-18 | Method for growing seedling of lavender |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6291122A true JPS6291122A (en) | 1987-04-25 |
Family
ID=16960705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60233796A Pending JPS6291122A (en) | 1985-10-18 | 1985-10-18 | Method for growing seedling of lavender |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6291122A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0257130A (en) * | 1988-08-19 | 1990-02-26 | Fuji Spinning Co Ltd | Production of adventitious embryo and/or adventitious bud of jasmine plant |
| CN102763546A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Lavandula angustifolia on loam land parcels |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
| CN104186292A (en) * | 2014-09-11 | 2014-12-10 | 丹阳市世外桃源果品专业合作社 | Cuttage planting method of lavender |
| CN106212005A (en) * | 2016-08-01 | 2016-12-14 | 苏州市清水村生态农副产品生产经营专业合作社 | A kind of implantation methods of lavandula angustifolia green high yield |
-
1985
- 1985-10-18 JP JP60233796A patent/JPS6291122A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0257130A (en) * | 1988-08-19 | 1990-02-26 | Fuji Spinning Co Ltd | Production of adventitious embryo and/or adventitious bud of jasmine plant |
| CN102763546A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Lavandula angustifolia on loam land parcels |
| CN102823492A (en) * | 2012-08-08 | 2012-12-19 | 中国科学院武汉植物园 | Method for quickly propagating lavenders |
| CN104186292A (en) * | 2014-09-11 | 2014-12-10 | 丹阳市世外桃源果品专业合作社 | Cuttage planting method of lavender |
| CN106212005A (en) * | 2016-08-01 | 2016-12-14 | 苏州市清水村生态农副产品生产经营专业合作社 | A kind of implantation methods of lavandula angustifolia green high yield |
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