CN116920002A

CN116920002A - Mi Luo Mu extract with efficacy of removing erythema, and preparation method and application thereof

Info

Publication number: CN116920002A
Application number: CN202310631307.0A
Authority: CN
Inventors: 林飞武; 朱统臣; 林椰
Original assignee: Guangzhou Jiahua Chemical Co ltd
Current assignee: Guangzhou Jiahua Chemical Co ltd
Priority date: 2023-05-30
Filing date: 2023-05-30
Publication date: 2023-10-24
Anticipated expiration: 2043-05-30
Also published as: CN116920002B

Abstract

The application relates to the technical field of plant extracts, in particular to a Mi Luo Mu extract with an erythema dispelling effect, a preparation method and application thereof. The method comprises the following steps: (1) Drying and crushing the honey Luo Mu to obtain honey Luo Mu raw material powder; (2) Adding a mixture of pectase, cellulase and protease into the raw material powder of the honey Luo Mu obtained in the step (1), and adding water to react for 1-3 days at the temperature of 30-40 ℃ to obtain an enzymolysis product; (3) Adding a mixed solvent into the enzymolysis product obtained in the step (2) for ultrasonic extraction treatment, centrifuging after ultrasonic extraction is finished, taking supernatant and filtering to obtain filtrate; (4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain a dense Luo Mu extract. The extract of Mi Luo Mu prepared by the application has the effects of removing spots and relieving when being applied to the field of skin care products, and can effectively remove the red spots on the surface of the skin.

Description

Mi Luo Mu extract with efficacy of removing erythema, and preparation method and application thereof

Technical Field

The application relates to the technical field of plant extracts, in particular to a Mi Luo Mu extract with an erythema dispelling effect, a preparation method and application thereof.

Background

The disclosure of this background section is only intended to increase the understanding of the general background of the application and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.

Density Luo Mu (Myrothamnus flabellifolia) is a leaf-flavored shrub native to the south Africa, an extremely drought tolerant plant, and the only woody resuscitated plant on earth. The density Luo Mu can resist high dehydration and drying, and can still keep the characteristic of reviving when meeting water when the water content of the tissue is only about ten percent. The dehydrated dense Luo Mu leaves become brown and pass through a severe drought period in a dormant mode, and the dry brown leaves quickly absorb water during rehydration, and can turn green and restore viability only by about 1 day.

There are various reasons that may lead to erythema on the skin of people, which may be classified as allergic, infectious or other disease-causing depending on the causative factors. Allergic erythema is usually skin erythema caused by exposure to allergens, and causes of infectious erythema are mainly skin infections such as bacterial and viral infections, such as erythema eruption, viral rash, and the like, and skin erythema caused by sun exposure.

Few studies in the prior art have involved the extraction of components from Mi Luo Mu and their application to the field of skin care products, nor the prior art has applied Mi Luo Mu to the field of removing skin erythema.

Disclosure of Invention

In order to solve the technical problems in the prior art, the application discloses a Mi Luo Mu extract with the efficacy of removing erythema, and a preparation method and application thereof, and the Mi Luo Mu extract prepared by the method disclosed by the application has the efficacy of removing erythema and can be applied to the field of skin care products.

Specifically, the technical scheme of the application is as follows:

a preparation method of Mi Luo Mu extract with erythema dispelling effect comprises the following steps:

(1) Drying and crushing the honey Luo Mu to obtain honey Luo Mu raw material powder;

(2) Adding a mixture of pectase, cellulase and protease into the raw material powder of the honey Luo Mu obtained in the step (1), and adding water to react for 1-3 days at the temperature of 30-40 ℃ to obtain an enzymolysis product;

(3) Adding a mixed solvent into the enzymolysis product obtained in the step (2) for ultrasonic extraction treatment, centrifuging after ultrasonic extraction is finished, taking supernatant and filtering to obtain filtrate;

(4) Concentrating the filtrate obtained in the step (3) under reduced pressure to obtain a dense Luo Mu extract.

In a second aspect of the present application, there is provided a mi Luo Mu extract prepared by the method for preparing a mi Luo Mu extract with erythema-dispelling effect of the first aspect.

In a third aspect of the application there is provided the use of the extract of Mi Luo Mu of the second aspect in the field of skin care products.

The application has the following beneficial effects:

the application creatively extracts the components in the Mimi Luo Mu and applies the components to the field of skin care products, and finds the efficacy of the Mimi Luo Mu extract in removing skin erythema.

According to the preparation method of the extract of the Mi Luo Mu with the efficacy of removing erythema, the mixture of pectase, cellulase and protease is adopted to decompose the raw material of the Mi Luo Mu, so that the nutrients such as starch, lipid, vitamin and protein in the Mi Luo Mu cells can be fully released, and the nutrients of macromolecules can be decomposed into the nutrients of small molecules, and the absorption of the skin to the nutrients in the extract of the Mi Luo Mu can be promoted, so that the effect of improving the erythema of the skin can be achieved. In the application, 50-70% ethanol water solution is used as a mixed solvent to ultrasonically extract the enzymolysis product of the honey Luo Mu, and most of the effective components in the enzymolysis product of the honey Luo Mu can be extracted through the operation.

The Mi Luo Mu extract with the erythema-dispelling effect, which is prepared by the preparation method of the Mi Luo Mu extract with the erythema-dispelling effect, can effectively relieve skin and reduce skin erythema caused by acne, allergy and the like.

Detailed Description

It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present application. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.

As discussed in the background, none of the prior art has extracted the ingredients of mi Luo Mu and applied to the field of skin care products, nor has it applied mi Luo Mu to the field of removing skin erythema. The present application provides a preparation method of Miao Luo Mu extract and application thereof in treating skin erythema, and has remarkable effects.

In one embodiment of the application, a preparation method of a Mi Luo Mu extract with erythema dispelling effect is provided, comprising the following steps:

In the preparation method, the raw material of the honey Luo Mu is decomposed by adopting a mixture of pectase, cellulase and protease, and the pectase and the cellulase are matched for use, so that components such as pectin and cellulose in the cell wall of the honey Luo Mu can be degraded, starch, lipids, vitamins and proteins in the cell of the honey Luo Mu are released, and the starch can be decomposed to generate oligosaccharides and monosaccharides. The added protease can decompose the protein released by the density Luo Muzhong to generate polypeptide and amino acid. The small molecular nutrient substances generated by the complex enzyme hydrolysis density Luo Mu can be better absorbed by skin, so that the effect of improving skin erythema is achieved.

In a specific embodiment, the temperature at which the seal Luo Mu is dried in step (1) is from 40 to 60 ℃.

In a specific embodiment, the mass ratio of the raw material powder of Mi Luo Mu, pectase, cellulase and protease is 8-10:1.5:1:0.5.

In a specific embodiment, the mixed solvent is an ethanol aqueous solution, and preferably, the volume fraction of ethanol in the ethanol aqueous solution is 50% -70%.

In a specific embodiment, the ratio of the enzymatic hydrolysate to the mixed solvent is 1:60-80 g/ml, preferably 1:65-75 g/ml, and more preferably 1:70 g/ml.

In a specific embodiment, the ultrasonic power of ultrasonic extraction in the step (3) is 400-600W, the ultrasonic extraction time is 20-30 min, and the ultrasonic extraction temperature is 60-80 ℃;

preferably, the power of the ultrasonic wave is 450-550W, the ultrasonic extraction time is 22-28 min, and the ultrasonic extraction temperature is 65-75 ℃.

The enzymolysis product of the known high-density Luo Mu contains various nutritional components and has very complex components, so that a proper solvent is required to extract the effective components in the enzymolysis product. In the application, 50-70% ethanol water solution is used as a mixed solvent for ultrasonic extraction of the enzymolysis product of the honey Luo Mu, most of the effective components in the enzymolysis product of the honey Luo Mu can be extracted by the operation, and ethanol in the enzymolysis product can be removed by decompression concentration, so that the extract of the honey Luo Mu can be obtained.

In a specific embodiment, the reduced pressure concentration in step (4) is performed at a temperature of 40 to 60 ℃ and a pressure of-0.2 to-0.1 MPa.

In one embodiment of the application, a Mi Luo Mu extract prepared from Mi Luo Mu extract with erythema dispelling effect is provided.

In one embodiment of the application, the application of the extract of the honey Luo Mu in the field of skin care products is provided.

The present application will be further illustrated with reference to the following specific examples, but the present application is not limited to the following examples. The methods are conventional methods unless otherwise specified.

Example 1

(1) Drying and crushing the honey Luo Mu at the temperature of 40 ℃ to obtain honey Luo Mu raw material powder;

(2) Adding a mixture of pectase, cellulase and protease with the mass ratio of 8:1.5:1:0.5 into the raw material powder of the honey Luo Mu obtained in the step (1), and adding water to react for 1 day at the temperature of 30 ℃ to obtain an enzymolysis product;

(3) Adding 50% ethanol water solution into the enzymolysis product obtained in the step (2) to carry out ultrasonic extraction treatment, wherein the ultrasonic power of ultrasonic extraction is 400W, the ultrasonic extraction time is 20min, the ultrasonic extraction temperature is 60 ℃, centrifuging treatment is carried out after ultrasonic extraction is completed, and supernatant liquid is taken and filtered to obtain filtrate;

(4) Concentrating the filtrate obtained in the step (3) under reduced pressure at 40 ℃ and under-0.2 MPa to obtain the extract of Mi Luo Mu.

Example 2

(1) Drying and crushing the honey Luo Mu at 50 ℃ to obtain honey Luo Mu raw material powder;

(2) Adding a mixture of pectase, cellulase and protease with the mass ratio of 9:1.5:1:0.5 into the raw material powder of the honey Luo Mu obtained in the step (1), and adding water to react for 2 days at the temperature of 35 ℃ to obtain an enzymolysis product;

(3) Adding an ethanol water solution with the ethanol volume fraction of 60% into the enzymolysis product obtained in the step (2) for ultrasonic extraction treatment, wherein the ultrasonic power of ultrasonic extraction is 500W, the ultrasonic extraction time is 25min, the ultrasonic extraction temperature is 70 ℃, centrifuging treatment is carried out after ultrasonic extraction is completed, and supernatant liquid is taken and filtered to obtain filtrate;

(4) Concentrating the filtrate obtained in the step (3) under reduced pressure at 50deg.C under-0.15 MPa to obtain extract of Mi Luo Mu.

Example 3

(1) Drying and crushing the honey Luo Mu at 55 ℃ to obtain honey Luo Mu raw material powder;

(2) Adding the raw material powder of the density Luo Mu obtained in the step (1) into the raw material powder of the density Luo Mu in a mass ratio of 9.5:1.5:1: 0.5, adding water to react for 2.5 days at 38 ℃ to obtain an enzymolysis product;

(3) Adding ethanol water solution with ethanol volume fraction of 68% into the enzymolysis product obtained in the step (2) for ultrasonic extraction treatment, wherein the ultrasonic power of ultrasonic extraction is 550W, the ultrasonic extraction time is 28min, the ultrasonic extraction temperature is 75 ℃, centrifuging treatment is carried out after ultrasonic extraction is completed, and supernatant is taken and filtered to obtain filtrate;

(4) Concentrating the filtrate obtained in the step (3) under reduced pressure at 55deg.C under-0.2 MPa to obtain extract of Mi Luo Mu.

Example 4

(1) Drying and crushing the honey Luo Mu at 60 ℃ to obtain honey Luo Mu raw material powder;

(2) Adding the raw material powder of the density Luo Mu obtained in the step (1) into the raw material powder of the density Luo Mu in a mass ratio of 10:1.5:1: 0.5, adding water to react for 3 days at the temperature of 40 ℃ to obtain an enzymolysis product;

(3) Adding 50-70% ethanol water solution into the enzymolysis product obtained in the step (2) to carry out ultrasonic extraction treatment, wherein the ultrasonic power of ultrasonic extraction is 600W, the ultrasonic extraction time is 30min, the ultrasonic extraction temperature is 80 ℃, centrifuging treatment is carried out after ultrasonic extraction is completed, and supernatant liquid is taken and filtered to obtain filtrate;

(4) Concentrating the filtrate obtained in the step (3) under reduced pressure at 60 ℃ and under-0.1 MPa to obtain the extract of Mi Luo Mu.

Test examples

Anti-inflammatory efficacy test of extract of mi Luo Mu:

the testing method comprises the following steps: the macrophages of the mice are obtained by culturing, and lipopolysaccharide LPS is used for stimulating the macrophages of the mice, so that the macrophages of the mice secrete inflammatory factor TNF-alpha. The extract of Mi Luo Mu prepared by the embodiment of the application can inhibit macrophage from secreting inflammatory factor TNF-alpha. The inhibition of inflammatory factor TNF- α release by the extract of mi Luo Mu was evaluated by measuring the amount of inflammatory factor TNF- α in the supernatant of the mouse macrophage culture fluid. The evaluation criterion was calculated to show that the higher the inhibition ratio (%) of inflammatory factor TNF-alpha, the better the inflammation inhibition effect of the extract of Mi Luo Mu was. The test results are shown in Table 1.

TABLE 1

	TNF-alpha inhibition rate
		Example 1	53.6％
Example 2	54.1％
		Example 3	52.9％
Example 4	53.8％

As can be seen from Table 1, the inhibition ratio of the extract of Mi Luo Mu prepared in examples 1-4 of the present application to inflammation was higher than 50%, which indicates that the extract of Mi Luo Mu prepared in the present application has good anti-inflammatory effect.

Antiallergic efficacy test of Mi Luo Mu extract:

histamine is a small molecular weight amine, mast cells stored in tissues and basic cells in the circulation. When the human body develops antigen-antibody reaction or anaphylaxis, the mast cells are stimulated to release histamine, so that the antiallergic effect of the extract of Mi Luo Mu can be evaluated by detecting the release of histamine. This example uses KU812 cells, which are highly similar to mast cells, to conduct an antiallergic efficacy test of the extract of mi Luo Mu. KU812 cells were cultured to adhere, the culture solution was added with the extract of mi Luo Mu prepared in the example of the application for further culture, the test group was added with C5a to stimulate the release of histamine from mast cells, and a blank group was set. After completion of the reaction, the supernatant of the culture broth and the cell pellet were separated, the histamine content was measured by a fluorescence method, and the resultant histamine inhibition was calculated, and the results were shown in Table 2.

TABLE 2

	Histamine inhibition rate
		Example 1	43.5％
Example 2	42.6％
		Example 3	42.9％
Example 4	43.2％

As can be seen from Table 2, the extract of Mi Luo Mu prepared in the example of the present application has high histamine inhibition rate, and can achieve antiallergic effect, thereby effectively inhibiting erythema caused by anaphylaxis.

Spot-removing efficacy test of mi Luo Mu extract:

the testing method comprises the following steps: 20 volunteers with skin erythema of different degrees caused by acne were equally divided into four groups, wherein two random groups of volunteers were applied with the Luo Mimu extract prepared by the example of the present application after cleansing with clear water daily in the morning and evening, the other two groups of volunteers were applied with some existing brand of freckle-removing skin care product after cleansing with clear water daily in the morning and evening, the number of skin erythema of the volunteers was detected from day 0 and day 7 of the beginning of use, respectively, and the reduction rate of erythema was calculated. The test results are shown in Table 3.

TABLE 3 Table 3

As can be seen from table 3, luo Mimu extract prepared according to the present application was effective in reducing the number of erythema on the skin surface of volunteers.

The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A preparation method of a Mi Luo Mu extract with erythema dispelling effect, which is characterized by comprising the following steps:

2. The process according to claim 1, wherein the temperature at which the density Luo Mu is dried in step (1) is from 40 to 60 ℃.

3. The method according to claim 1, wherein the mass ratio of raw material powder of up to Luo Mu, pectase, cellulase and protease is 8-10:1.5:1:0.5.

4. The method according to claim 1, wherein the mixed solvent is an aqueous ethanol solution.

5. The method according to claim 4, wherein the volume fraction of ethanol in the aqueous ethanol solution is 50% to 70%.

6. The method according to claim 1, wherein the ratio of the enzymatic hydrolysate to the mixed solvent is 1:60-80 g/ml.

7. The preparation method according to claim 1, wherein the ultrasonic power of ultrasonic extraction in the step (3) is 400-600W, the ultrasonic extraction time is 20-30 min, and the ultrasonic extraction temperature is 60-80 ℃.

8. The method according to claim 1, wherein the reduced pressure concentration in the step (4) is carried out at a temperature of 40 to 60℃and a pressure of-0.2 to-0.1 MPa.

9. The extract of Mi Luo Mu having erythema-dispelling effect prepared by the method of any one of claims 1 to 8.

10. Use of the extract of mi Luo Mu with erythema-dispelling effect according to claim 9 in the safety evaluation or efficacy evaluation of pharmaceutical or cosmetic products.