CN116893131B - Flow type sample analyzer and flow chamber cleaning method - Google Patents
Flow type sample analyzer and flow chamber cleaning method Download PDFInfo
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- CN116893131B CN116893131B CN202311165959.6A CN202311165959A CN116893131B CN 116893131 B CN116893131 B CN 116893131B CN 202311165959 A CN202311165959 A CN 202311165959A CN 116893131 B CN116893131 B CN 116893131B
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- 238000004140 cleaning Methods 0.000 title claims abstract description 180
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 317
- 239000012530 fluid Substances 0.000 claims abstract description 85
- 238000002347 injection Methods 0.000 claims abstract description 73
- 239000007924 injection Substances 0.000 claims abstract description 73
- 239000002699 waste material Substances 0.000 claims abstract description 56
- 230000009471 action Effects 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 13
- 238000007599 discharging Methods 0.000 claims description 29
- 238000002791 soaking Methods 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 19
- 238000009792 diffusion process Methods 0.000 claims description 13
- 230000002159 abnormal effect Effects 0.000 claims description 9
- 238000000429 assembly Methods 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims description 3
- 238000007872 degassing Methods 0.000 claims description 3
- 238000013022 venting Methods 0.000 claims description 3
- 238000010926 purge Methods 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 abstract description 2
- 239000000306 component Substances 0.000 description 64
- 238000004891 communication Methods 0.000 description 21
- 238000010586 diagram Methods 0.000 description 8
- 239000012535 impurity Substances 0.000 description 6
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- 230000009286 beneficial effect Effects 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention provides a flow type sample analyzer and a flow chamber cleaning method, which are suitable for the technical field of medical detection equipment. The present invention provides a flow sample analyzer comprising: the sheath flow tank comprises a sample liquid inlet, a sheath liquid inlet and a waste liquid outlet, a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline; the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline; and the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning fluid in the sample injection pipeline to the sample needle, and the second power component is used for alternately executing the exhausting and sucking actions, so that the exhaust amount is larger than the sucking amount, the cleaning fluid and the sheath fluid mixed solution are pushed into the sheath flow pool again, the bubbles in the sheath flow pool are effectively eliminated, the pressure stability in the flow chamber is ensured, the sample flow is further stable, and the detection result is more accurate.
Description
Technical Field
The invention belongs to the technical field of medical detection equipment, and particularly relates to a flow type sample analyzer and a flow chamber cleaning method.
Background
The flow detection technology is a technology for distinguishing particles with different properties, such as microspheres or cells and the like in suspension, such as different marking fluorescent signal intensities, different colors, different particle sizes and the like, by a laser detection mode, and finally counting and sorting. The technology has the characteristics of high precision, high sensitivity and support of simultaneous detection of multiple channels, and is widely applied to the field of medical examination. The core component of the technology is a flow chamber component, and a continuous and stable sample flow in the flow chamber is a precondition for ensuring the stability and accuracy of a test result. Therefore, a complete and effective scheme for ensuring the stable maintenance of the sample flow in the flow chamber is a key for improving the stability of the flow technology.
The formation of a stable sample flow requires the establishment of a stable pressure, which slightly fluctuates to cause sample flow anomalies, and bubble generation and retention can lead to unstable pressure within the flow chamber, thereby affecting sample flow stability.
Disclosure of Invention
The embodiment of the invention provides a flow type sample analyzer, which aims to solve the problem that the pressure in a flow chamber is unstable due to bubble generation and retention, so that the stability of a sample flow is affected.
The embodiment of the invention is realized in such a way that a flow sample analyzer comprises:
The sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle, and controlling the second power component to alternately execute the air exhaust and suction actions so as to eliminate air bubbles in the sheath flow tank and discharge the cleaning liquid from the waste liquid outlet; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount.
Optionally, before the controller controls the second power assembly to alternately perform the degassing and aspiration actions to eliminate bubbles in the sheath flow cell, the controller is further configured to:
controlling the second power assembly to suck in so as to suck down the cleaning liquid discharged into the sheath flow reservoir by the sample needle to soak the sheath flow reservoir, wherein the second time period of the cleaning liquid soaking the sheath flow reservoir is longer than the time period of the cleaning liquid diffusing to the second power assembly.
Optionally, the second time period is determined by the length of the sheath fluid pipeline and the diffusion speed of the cleaning fluid, and the second time period satisfies the following relationship:
wherein:
t is a second duration;
l is the length of the sheath liquid pipeline;
v is the diffusion rate of the cleaning liquid.
Optionally, the volume of the sheath fluid pipeline is greater than or equal to 1/2 of the volume of the sheath flow cell.
Optionally, before the controller controls the first power component to push the cleaning solution in the sample injection pipeline to the sample needle, the controller is further configured to:
controlling the first power assembly to clean the sample injection pipeline;
the controller is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle, and is also used for:
and controlling the first power assembly and the second power assembly to fill the sheath flow tank with sheath liquid.
Optionally, controlling the first power assembly to clean the sample injection pipeline includes:
controlling the first power assembly to inhale, inhaling the cleaning liquid into the sample introduction pipeline, and soaking for a first period of time;
controlling the first power assembly to alternately exhaust and inhale, and cleaning the sample injection pipeline;
and controlling the first power assembly to exhaust, and discharging the cleaning liquid out of the sample injection pipeline.
Optionally, after the controller executes the control of the first power component to push the cleaning solution in the sample injection pipeline to the sample needle, the controller is further configured to:
controlling the first power assembly to suck air, and sucking the air into the sample injection pipeline;
controlling the first power assembly to vent, venting air from the sample needle into the sheath flow cell;
controlling the first power assembly to suck air, and sucking the cleaning liquid into the sample injection pipeline;
and controlling the first power assembly to exhaust, and discharging the cleaning liquid from the sample needle into the sheath flow cell.
Optionally, controlling the first power assembly and the second power assembly, the sheath flow cell being filled with sheath fluid, includes:
controlling the second power assembly to exhaust, and discharging the cleaning liquid in the sheath liquid pipeline;
controlling the second power assembly to suck air, and sucking sheath fluid into the second power assembly;
controlling the first power assembly to suck air, and sucking sheath fluid into the first power assembly;
and controlling the second power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, adjusting the first valve assembly to be at a first working position by the controller, controlling the first power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, and extruding redundant liquid from a waste liquid port.
Optionally, the flow sample analyzer further comprises a fault detection module, configured to detect an abnormal condition of the flow sample analyzer;
the controller is also used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle when the abnormal condition is related to the existence of bubbles in the flow chamber, and controlling the second power component to alternately execute the action of exhausting and sucking so as to eliminate the bubbles in the sheath flow tank.
The embodiment of the invention also provides a flow chamber cleaning method which is applied to the flow sample analyzer, and the flow sample analyzer comprises the following steps: the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline; the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline; the controller is respectively connected with the first power assembly and the second power assembly;
The flow chamber cleaning method comprises the following steps:
controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharging the cleaning liquid into the sheath flow cell through the sample needle;
controlling the second power assembly to alternately perform an air exhausting and sucking action so as to eliminate air bubbles in the sheath flow cell and discharge the cleaning liquid from the waste liquid port; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount.
The embodiment of the invention also provides a flow sample analyzer, which comprises:
the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
and the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle, discharging the cleaning liquid into the sheath flow tank through the sample needle, controlling the second power component to suck in the cleaning liquid discharged into the sheath flow tank by the sample needle to soak the sheath flow tank, and discharging the cleaning liquid from the waste liquid outlet by controlling the second power component to exhaust after soaking for a second period of time.
The invention has the beneficial effects that as the sample injection pipeline communicated with the first power component and the sheath liquid pipeline communicated with the second power component are arranged, the controller is used for controlling the air suction and the air discharge of the first power component and the second power component, the air discharge amount is larger than the air suction amount when the second power component alternately executes the air discharge and air suction actions, the cleaning liquid and sheath liquid mixed liquid can be pushed into the sheath flow pool again, the bubbles in the sheath flow pool can be effectively eliminated, the pressure stability in the flow chamber can be ensured, the sample flow is stable, and the detection result is more accurate.
Drawings
FIG. 1 is a schematic diagram of a flow sample analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a flow sample analyzer according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a flow chart of a sample analyzer according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of another flow chart sample analyzer according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of another flow chart sample analyzer according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of another flow chart sample analyzer according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of another flow chart of a sample analyzer according to an embodiment of the present invention.
Reference numerals illustrate:
101. a sheath flow cell; 1011. a sample liquid inlet; 1012. a sheath fluid inlet; 1013. a waste liquid port; 1014. a sample needle;
102. a waste liquid pipeline; 103. a second switching valve; 104. a sample injection pipeline; 105. a branch pipeline; 106. sample position; 107. a first valve assembly; 108. a first power assembly; 109. a first switching valve; 110. a sheath fluid pipeline; 111. a second valve assembly; 112. a second power assembly; 113. a sheath fluid reservoir; 114. and a controller.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. Examples of the embodiments are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements throughout or elements having like or similar functionality. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention. Furthermore, it should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the present invention.
In the description of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "left," "right," "horizontal," "top," "bottom," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.
The following disclosure provides many different embodiments, or examples, for implementing different structures of the invention. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.
According to the invention, as the sample injection pipeline communicated with the first power component and the sheath liquid pipeline communicated with the second power component are arranged, the controller is used for controlling the air suction and the air discharge of the first power component and the second power component, so that the sheath flow pool is cleaned, the bubbles in the sheath flow pool are eliminated, the pressure in the flow chamber is ensured to be stable, the sample flow is further stable, and the detection result is more accurate.
Examples
The present embodiment provides a flow sample analyzer, including:
The sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle, and controlling the second power component to alternately execute the air exhaust and suction actions so as to eliminate air bubbles in the sheath flow tank and discharge the cleaning liquid from the waste liquid outlet; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount.
Specifically, as shown in fig. 1 and 2, the sheath flow cell 101 includes a sample inlet 1011 formed at the bottom of the sheath flow cell 101, a sheath liquid inlet 1012 formed at the lower side wall, a waste liquid port 1013 formed at the top, a sample needle 1014 formed in the sheath flow cell 101, one end of the sample needle 1014 being communicated with the sample inlet 1011, the waste liquid port 1013 being communicated with the waste liquid line 102, and a second switch valve 103 formed on the waste liquid line 102;
And a sample feeding pipeline 104, wherein a branch pipeline 105 is arranged in the sample feeding pipeline 104, and the branch pipeline 105 is communicated with the sample liquid inlet 1011. One end of the sample injection pipeline 104 is connected with a sample position 106, the other end of the sample injection pipeline 104 is connected with a liquid inlet of a first valve assembly 107, a working port of the first valve assembly 107 is connected with a first power assembly 108, a first switch valve 109 is arranged on the sample injection pipeline 104 between the sample position 106 and the branch pipeline 105, and a liquid outlet of the first valve assembly 107 is connected with a sheath liquid pool 113;
the sheath liquid pipeline 110 comprises a sheath liquid pipeline 110 and a sheath liquid rear pipe, one end of the sheath liquid pipeline 110 is connected with a sheath liquid inlet 1012, the other end is connected with a liquid inlet of the second valve assembly 111, a working port of the second valve assembly 111 is connected with the second power assembly 112, and a liquid outlet of the second valve assembly 111 is connected with the sheath liquid pool 113;
the controller 114 is respectively in communication connection with the first switch valve 109, the second switch valve 103, the first valve assembly 107, the second valve assembly 111, the first power assembly 108 and the second power assembly 112;
wherein, valve assembly is in first working position feed liquor mouth and working opening intercommunication, and in second working position leakage fluid dram and working opening intercommunication.
In this embodiment, sheath flow is a technique in which a capillary tube is aligned with a small hole tube, and the cell suspension is ejected from the capillary tube and simultaneously flows through a sensitive area together with the sheath liquid flowing around, so as to ensure that the cell suspension forms a single cell flow in the middle. To ensure that the cell suspension forms a single aligned stream of cells in the middle, the cell suspension is surrounded by sheath fluid, so the sheath fluid reservoir 101 is filled with sheath fluid.
The sample needle 1014 is disposed in the sheath flow cell 101, the sample needle 1014 is connected to the sample liquid inlet 1011 at the bottom of the sheath flow cell 101, and the liquid introduced into the sheath flow cell 101 through the sample liquid inlet 1011 needs to be introduced into the sheath flow cell 101 through the sample needle 1014. The waste liquid port 1013 is provided at the top of the sheath flow cell 101, and since the sheath flow cell 101 is filled with sheath liquid, when the sheath flow cell 101 is excessively filled with the sheath liquid, the excess liquid can be discharged from the sheath flow cell 101 through the waste liquid port 1013.
One end of the sample feeding pipeline 104 is connected with the sample position 106, and the other end is connected with a liquid inlet of the first valve assembly 107. The working port of the first valve assembly 107 is connected to the first power assembly 108, and the drain port of the first valve assembly 107 is connected to the sheath fluid reservoir 113. When the first valve assembly 107 is in the first operating position, the first power assembly 108 is in communication with the sample line 104, and when the first valve assembly 107 is in the second operating position, the first power assembly 108 is in communication with the sheath fluid reservoir 113.
The sample placed in connection with sample site 106 may be replaced. The sample pipeline 104 is provided with a branch pipeline 105, the branch pipeline 105 is communicated with the sample liquid inlet 1011, and the sample pipeline 104 is communicated with the sheath flow cell 101 through the branch pipeline 105. A first switch valve 109 is provided in the sample line 104 between the sample site 106 and the bypass line 105. The first switch valve 109 controls the communication between the sample line 104 and the sample site 106, and when the first switch valve 109 is closed, the communication between the sample line 104 and the sample site 106 is blocked.
Sheath liquid pipeline 110, sheath liquid inlet 1012 is connected to sheath liquid pipeline 110's one end, and the inlet of second valve subassembly 111 is connected to the other end, and the operating port second power subassembly 112 of second valve subassembly 111, the operating port of second valve subassembly 111 is connected to the other end, and the leakage fluid dram of second valve subassembly 111 is connected with sheath liquid pond 113. The second power assembly 112 is in communication with the sheath fluid line 110 when the second valve assembly 111 is in the first operating position, and the second power assembly 112 is in communication with the sheath fluid reservoir 113 when the second valve assembly 111 is in the second operating position.
The controller 114 is respectively in communication connection with the first switch valve 109, the second switch valve 103, the first valve assembly 107, the second valve assembly 111, the first power assembly 108 and the second power assembly 112, and the controller 114 respectively controls the first switch valve 109, the second switch valve 103 to open and close, respectively controls the working positions of the first valve assembly 107 and the second valve assembly 111, and respectively controls the first power assembly 108 and the second power assembly 112 to suck or exhaust.
The controller 114 controls the first switch valve 109, the second switch valve 103, the first valve assembly 107, the second valve assembly 111, the first power assembly 108 and the second power assembly 112 to clean the sheath flow cell 101.
Specifically, the first power component 108 and the second power component 112 may be suction pumps, or may be injectors with push handles driven by motors, or may be other common devices capable of implementing air extraction and air exhaust functions.
The first valve assembly 107 and the second valve assembly 111 may be single valves having two working positions to realize two-position three-way, or may be assemblies formed by individual valves.
In one embodiment, the volume of sheath fluid circuit 110 is greater than or equal to 1/2 of the volume of sheath fluid cell 101.
In this embodiment, since the sheath flow cell 101 is initially filled with the sheath liquid, the sample needle 1014 is immersed in the sheath liquid, and the waste liquid port 1013 is provided at the top of the sheath flow cell 101, and the excess liquid can be discharged only from the top of the sheath flow cell 101, the mixed liquid of the cleaning liquid and the sheath liquid can always submerge the sample needle 1014 after the cleaning liquid is injected into the sheath flow cell 101. Typically, the length of the sample needle 1014 is between 50% and 90% of the height of the sheath flow cell 101, and the specific length of the sample needle 1014 varies from model to model of sheath flow cell 101.
One end of the sheath fluid pipeline 110 is connected with the side wall of the lower part of the sheath fluid tank 101, and the other end is communicated with the second power assembly 112 through the second valve assembly 111, so that when the second power assembly 112 is used for exhausting air, the liquid in the sheath fluid tank 101 can be introduced into the sheath fluid pipeline 110. Because cleaning fluids are typically corrosive, they tend to corrode components (e.g., seals) within the second power assembly 112. In order to avoid corrosion of the second power assembly 112 caused by pumping the cleaning solution into the second power assembly 112 when the second power assembly 112 is pumped, the volume of the sheath fluid pipeline 110 is larger than the volume of the mixed solution of the cleaning solution and the sheath fluid after the cleaning solution is injected.
Specifically, when the volume of the liquid in the sheath flow cell 101 after the cleaning liquid is injected into the sheath flow cell 101 is 1/2 of the volume of the sheath flow cell 101, the volume of the sheath liquid piping 110 is greater than or equal to 1/2 of the volume of the sheath flow cell 101.
When the volume of the liquid in the sheath flow tank 101 after the cleaning liquid is injected into the sheath flow tank 101 is 9/10 of the volume of the sheath flow tank 101, the volume of the sheath liquid piping 110 is greater than or equal to 9/10 of the volume of the sheath flow tank 101.
In one embodiment, the second power assembly 112 alternately exhausts and inhales once with an exhaust volume greater than the intake volume.
In this embodiment, when the second power assembly 112 performs the exhaust and suction alternating action each time, the exhaust amount is greater than the suction amount, and the suction action can make the cleaning liquid discharged into the sheath flow tank move down to the bottom of the sheath flow tank to completely soak the sheath flow tank, so that multiple rows of small suction are beneficial to pushing the cleaning liquid and the sheath liquid mixed liquid into the sheath flow tank 101 again, so that bubbles in the sheath flow tank are effectively eliminated, the pressure in the flow tank is ensured to be stable, the sample flow is further stable, and the detection result is more accurate; and avoid the washing liquid to spread to the second power component in sheath flow pipeline, can avoid producing the influence to the instrument when guaranteeing instrument cleaning effect.
In one embodiment, the valve assembly is a two-position three-way valve.
In this embodiment, the first valve assembly 107 is a first two-position three-way valve and the second valve assembly 111 is a second two-position three-way valve.
Two-position three-way valves are a common type of valve that is used primarily to control the flow direction of a fluid. The device is provided with three connectors, two connecting positions, and one connector is respectively communicated with the other two connectors when the two connecting positions are different. Different fluid flow control is achieved by changing the spool position within the valve.
The two-position three-way valve is widely applied to the fields of industry and construction, has the advantages of simple structure, convenient operation, high reliability and the like, and is suitable for various media and working conditions. In the embodiment, a two-position three-way valve is selected, so that the operation is convenient, and the manufacturing cost is low.
In one embodiment, before the controller controls the second power assembly to alternately perform the degassing and aspiration actions to eliminate bubbles in the sheath flow cell, the controller is further configured to:
controlling the second power assembly to suck in so as to suck down the cleaning liquid discharged into the sheath flow reservoir by the sample needle to soak the sheath flow reservoir, wherein the second time period of the cleaning liquid soaking the sheath flow reservoir is longer than the time period of the cleaning liquid diffusing to the second power assembly.
The second power unit 112 is controlled to suck air, and at this time, the air pressure in the sheath flow reservoir 101 is reduced, and the cleaning liquid splashed on the wall of the sheath flow reservoir 101 and on the upper portion of the sheath flow reservoir 101 is sucked into the bottom of the sheath flow reservoir, and immersed for a second period of time. The second power assembly 112 sucks air, which helps to ensure that the cleaning liquid can completely soak the sheath flow tank, so that the surface tension of air bubbles generated on the inner wall of the sheath flow tank is changed to break, and impurities such as particles or proteins in the sheath flow tank are effectively melted away. Wherein the determination of the second time period is similar to the determination of the first time period and is determined according to the specific selection of the cleaning liquid.
In one embodiment, the second length of time is determined by the length of the sheath fluid line 110 and the rate of cleaning fluid diffusion.
In this embodiment, the second period is a soaking period of the cleaning liquid in the sheath liquid pipeline 110, and the soaking helps to clean the sheath liquid pipeline 110.
Experiments prove that the second time length meets the following relation:
wherein:
t is a second duration;
l is the length of sheath fluid line 110;
v is the diffusion rate of the cleaning liquid.
In the sample analyzer shipped from the factory, the length of the sheath fluid line 110 is a constant value. The selected cleaning liquid is different in diffusion speed, the diffusion speed of the cleaning liquid is inversely related to the second time period, the faster the diffusion speed of the cleaning liquid is, the shorter the second time period is, the slower the diffusion speed of the cleaning liquid is, and the longer the second time period is.
For example, when the diffusion rate of the selected cleaning liquid is 100 mm/min, the sheath liquid pipeline length is 1000 mm, and at this time, the longest period of time allowed for soaking is less than 10 minutes; when the diffusion rate of the selected cleaning liquid is 150 mm/min, the length of the sheath liquid pipeline is 1800 mm, and at this time, the longest period of time allowed for soaking is less than 12 minutes, etc., and the present invention is not limited thereto.
By determining the second period of time for soaking according to the cleaning liquid diffusion rate and the sheath liquid pipe length, the cleaning liquid can be prevented from expanding to the sheath liquid injector while ensuring the cleaning effect, and corrosion is caused to the sheath liquid injector.
In one embodiment, the controller is further configured to, before controlling the first power assembly to push the cleaning solution in the sample injection line to the sample needle, control the first power assembly to:
controlling the first power assembly to clean the sample injection pipeline;
the controller is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle, and is also used for:
and controlling the first power assembly and the second power assembly to fill the sheath flow tank with sheath liquid.
As shown in fig. 3, the present embodiment provides a workflow diagram of a flow sample analyzer, which includes the following steps:
S101, controlling the first power assembly 108 to clean the sample injection pipeline 104;
s102, controlling the first power component 108 to introduce cleaning liquid into the sheath flow cell 101 through the sample liquid inlet 1011;
s103, controlling the second power assembly 112 to clean the sheath flow cell 101;
and S104, controlling the first power assembly 108 and the second power assembly 112 to fill the sheath flow tank 101 with sheath fluid.
Specifically, in step S101, the first power unit is controlled to clean the sample line 104, and this step is to clean the sample line 104 already installed in the system by operating the first switch valve 109, the second switch valve 103, the first valve unit 107 and the first power unit 108 in a predetermined flow path in relation to the communication of the sample line 104, and to remove impurities in the line.
In step S102, the first power unit is controlled to introduce the cleaning liquid from the sample inlet 1011 into the sheath flow cell 101, and in this step, the cleaning liquid is introduced from the sample inlet 1011 into the sheath flow cell 101 by the action of the first on-off valve 109, the second on-off valve 103, the first valve unit 107, and the first power unit 108 associated with the communication of the sheath flow cell 101 in a predetermined flow path. The cleaning fluid will pass through the tubing of the flow sample analyzer into the sheath flow cell 101, ready for subsequent cleaning of the sheath flow cell 101.
In step S103, the second power unit is controlled to clean the sheath flow cell 101, and the second valve unit 111 and the second power unit 112, which are in communication with the sheath flow cell 101, are operated in a predetermined flow path to clean the interior of the sheath flow cell 101. Sheath flow cell 101 is an important component of a flow sample analyzer and requires periodic cleaning to ensure proper operation. This can be done by introducing a cleaning liquid and discharging it after the cleaning is completed to remove impurities and dirt.
In step S104, the first power unit and the second power unit are controlled to fill the sheath flow cell 101 with sheath fluid, and in this step, the sheath fluid is injected into the sheath flow cell 101 until the sheath flow cell 101 is filled, by the action of the second valve unit 111 and the second power unit 112 in relation to the communication of the sheath flow cell 101, in a predetermined flow path. Sheath fluid is a specific fluid for the operation of a flow sample analyzer that provides a stable flow rate and pressure and protects the measurement device from the sample or other impurities.
The steps described above need to be performed sequentially to complete the cleaning of the flow cell.
In one embodiment, controlling the first power assembly to clean the sample line includes:
Controlling the first power assembly to inhale, inhaling the cleaning liquid into the sample introduction pipeline, and soaking for a first period of time;
controlling the first power assembly to alternately exhaust and inhale, and cleaning the sample injection pipeline;
and controlling the first power assembly to exhaust, and discharging the cleaning liquid out of the sample injection pipeline.
As shown in fig. 3 and 4, the specific steps may be:
s1011, controlling the first power assembly 108 to suck air, sucking the cleaning fluid into the sample injection pipeline 104, and soaking for a first period of time;
s1012, controlling the first power assembly 108 to alternately exhaust and inhale, and cleaning the sample injection pipeline 104;
s1013, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid out of the sample injection pipeline 104;
s102, controlling the first power component 108 to introduce cleaning liquid into the sheath flow cell 101 through the sample liquid inlet 1011;
s103, controlling the second power assembly 112 to clean the sheath flow cell 101;
and S104, controlling the first power assembly 108 and the second power assembly 112 to fill the sheath flow tank 101 with sheath fluid.
In the present embodiment, S1011 to S1014 are specific control flows for controlling the first power assembly 108 to clean the sample line 104 in step S101.
Specifically, in step S1011, a cleaning solution is placed on the sample site 106, the cleaning solution being used to clean impurities in the pipe or the cavity. The controller 114 adjusts the first valve assembly 107 to the first operating position such that the sample line 104 is in communication with the first power assembly 108. The first switch valve 109 is opened so that the sample site 106 communicates with the sample line 104. The second switch valve 103 is closed, so that the sheath flow tank 101 and the branch pipeline 105 are in a relatively airtight state, and the air pressure in the sheath flow tank 101 and the branch pipeline is in a stable state, so that external liquid is prevented from flowing in. I.e., the sample site 106, the sample line 104, and the first power assembly 108 are placed in communication.
The controller 114 controls the first power assembly 108 to inhale, i.e. the air pressure in the sample introduction pipeline 104 is reduced by the action of the first power assembly 108, and the cleaning solution placed at the sample position 106 is inhaled into the sample introduction pipeline 104 to soak for a first period of time. The first time length may be determined according to actual application requirements, which is not limited herein.
In step S1012, the controller 114 controls the first power component 108 to alternately exhaust and inhale, so that the cleaning solution washes and flows in the sample line 104, so as to clean the sample line 104, and at the same time, may take away bubbles that may adhere to the wall of the sample line 104.
In step S1013, the first power unit 108 is exhausted by mixing the impurities, bubbles, and the like in the cleaning liquid in S1011 and S1012, and the cleaning liquid in the sample line 104 is discharged out of the sample line 104, and the cleaning of the sample line 104 is completed.
In one embodiment, after the controller performs the controlling the first power component to push the cleaning solution in the sample injection line to the sample needle, the controller is further configured to:
controlling the first power assembly to suck air, and sucking the air into the sample injection pipeline;
controlling the first power assembly to vent, venting air from the sample needle into the sheath flow cell;
Controlling the first power assembly to suck air, and sucking the cleaning liquid into the sample injection pipeline;
and controlling the first power assembly to exhaust, and discharging the cleaning liquid from the sample needle into the sheath flow cell.
Specifically, as shown in fig. 3 to 5, a flow chart of a flow sample analyzer according to an embodiment of the present invention is provided, wherein step S102 includes steps S1021, S1022, S1023, and S1024, and the method includes the following steps:
s1011, controlling the first power assembly 108 to suck air, sucking the cleaning fluid into the sample injection pipeline 104, and soaking for a first period of time;
s1012, controlling the first power assembly 108 to alternately exhaust and inhale, and cleaning the sample injection pipeline 104;
s1013, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid out of the sample injection pipeline 104;
s1021, controlling the first power component 108 to suck air, and sucking the air into the sample injection pipeline 104;
s1022, controlling the first power assembly 108 to exhaust, and discharging air from the sample needle 1014 into the sheath flow cell 101;
s1023, controlling the first power assembly 108 to suck air, and sucking the cleaning fluid into the sample injection pipeline 104;
s1024, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid from the sample needle 1014 into the sheath flow cell 101;
S103, controlling the second power assembly 112 to clean the sheath flow cell 101;
and S104, controlling the first power assembly 108 and the second power assembly 112 to fill the sheath flow tank 101 with sheath fluid.
In this embodiment, S1021 to S1024 are specific control flows for the first power unit 108 in step S102 to introduce the cleaning liquid from the sample liquid inlet 1011 to the sheath flow cell 101.
In step S1021, the first valve assembly 107 is maintained at the first working position, the first switch valve 109 is opened, the second switch valve 103 is closed, and the sample position 106, the sample feeding line 104 and the first power assembly 108 are maintained in a communicating state. The cleaning solution on the sample site 106 is removed, so that the sample site 106 is empty, and the first power component 108 sucks air, i.e., sucks air into the sample line 104.
In step S1022, the controller 114 closes the first switch valve 109, such that the sample site 106 is disconnected from the sample line 104. The second switch valve 103 is opened, so that the inside of the sheath flow cell 101 and the branch pipeline 105 are not in a relatively airtight state any more, and can be communicated and exchanged with the outside. Since the connection between the sample line 104 and the sample site is blocked, the inlet and the outlet of the sheath flow cell 101 are both connected, i.e. the first power assembly 108, the sample line 104, the bypass line 105 and the sheath flow cell 101 are connected. The first power assembly 108 is controlled to exhaust, air in the sample injection pipeline 104 is discharged into the sheath flow cell 101 from the sample needle 1014 through the branch pipeline 105, and because the sheath flow cell 101 is originally filled with sheath liquid, the air enters the sheath flow cell 101 from the sample needle 1014, and the entering air occupies a part of the space in the sheath flow cell 101, so that the sheath liquid above the sample needle 1014 can be extruded from the waste liquid port 1013, and a space is reserved for the subsequent cleaning liquid to enter the sheath flow cell 101.
In step S1023, the cleaning solution is placed on the sample site 106, the controller 114 opens the first switch valve 109 and opens the second switch valve 103, i.e. the sample site 106, the sample line 104 and the first power assembly 108 are in a communication state. The first power assembly 108 is controlled to aspirate and aspirate the wash fluid placed at the sample site 106 into the sample line 104.
In step S1024, the controller 114 closes the first switching valve 109 and opens the second switching valve 103, so that the first power assembly 108, the sample line 104, the bypass line 105, and the sheath flow cell 101 are in communication. The first power unit 108 is controlled to discharge, and since a part of the sheath fluid is already squeezed out by the air in step S1023, the sheath fluid floats up in the sheath flow cell 101 due to the small air density, and the cleaning liquid is discharged from the sample needle 1014 into the sheath flow cell 101 closer to the waste liquid port 1013, and the air is squeezed out of the waste liquid port 1013. The sample liquid inlet 1011 is used for introducing the cleaning liquid into the sheath flow cell 101.
After the cleaning liquid enters the sheath flow tank 101, the cleaning liquid is prevented from being diffused, the sheath flow tank 101 is required to be cleaned, the rest maintenance actions except the cleaning are not performed in the middle as much as possible, and the interval time is controlled within 5 minutes.
In one embodiment, as shown in fig. 3 to 6, a flow chart of a flow sample analyzer according to an embodiment of the present invention is provided, wherein step S103 includes steps S103 1 and S1032, and the method includes the following steps:
S1011, controlling the first power assembly 108 to suck air, sucking the cleaning fluid into the sample injection pipeline 104, and soaking for a first period of time;
s1012, controlling the first power assembly 108 to alternately exhaust and inhale, and cleaning the sample injection pipeline 104;
s1013, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid out of the sample injection pipeline 104;
s1021, controlling the first power component 108 to suck air, and sucking the air into the sample injection pipeline 104;
s1022, controlling the first power assembly 108 to exhaust, and discharging air from the sample needle 1014 into the sheath flow cell 101;
s1023, controlling the first power assembly 108 to suck air, and sucking the cleaning fluid into the sample injection pipeline 104;
s1024, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid from the sample needle 1014 into the sheath flow cell 101;
s1031, controlling the second power assembly 112 to inhale, sucking the cleaning liquid splashed on the wall of the sheath flow tank 101 into the sheath flow tank 101, and soaking for a second period of time;
s1032, controlling the second power assembly 112 to alternately exhaust and inhale, and cleaning the sheath flow tank 101;
and S104, controlling the first power assembly 108 and the second power assembly 112 to fill the sheath flow tank 101 with sheath fluid.
In the present embodiment, S1031 and S1032 are specific control flows for controlling the second power assembly 112 to purge the sheath flow cell 101 in step S103.
Specifically, in step S1031, since the first switch valve 109 is closed and the state of the first valve assembly 107 is not changed, the sample line 104 is in a relatively airtight stable state, and is not easy to exchange with the liquid in the sheath flow cell 101. The controller 114 adjusts the second valve assembly 111 to the first operating state such that the sheath fluid line 110 and the second power assembly 112 are in communication. The second power assembly 112 is controlled to suck air, the air pressure in the sheath flow cell 101 is reduced at this time, and the cleaning liquid splashed on the wall of the sheath flow cell 101 and at the position of the sheath flow cell 101 close to the waste liquid port 1013 is sucked into the sheath flow cell 101 to be soaked for a second period of time. Wherein the determination of the second time period is similar to the determination of the first time period and is determined according to the specific selection of the cleaning liquid.
In step S1032, the second power assembly 112 alternately exhausts and sucks air, so that the cleaning liquid washes and flows in the sheath flow tank 101 and the sheath liquid pipeline 110, cleaning the sheath flow tank 101 and the sheath liquid pipeline 110, and simultaneously carrying away air bubbles possibly adhered to the walls of the sheath flow tank 101 and the sheath liquid pipeline 110, thereby completing cleaning the sheath flow tank 101.
In one embodiment, controlling the first and second power assemblies such that the sheath flow cell is filled with sheath fluid comprises:
Controlling the second power assembly to exhaust, and discharging the cleaning liquid in the sheath liquid pipeline;
controlling the second power assembly to suck air, and sucking sheath fluid into the second power assembly;
controlling the first power assembly to suck air, and sucking sheath fluid into the first power assembly;
and controlling the second power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, adjusting the first valve assembly to be at a first working position by the controller, controlling the first power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, and extruding redundant liquid from a waste liquid port.
Specifically, as shown in fig. 3 to 7, a flow chart of a flow sample analyzer according to an embodiment of the invention is provided, wherein step S104 includes steps S1041, S1042, S1043 and S1044, and the method includes the following steps:
s1011, controlling the first power assembly 108 to suck air, sucking the cleaning fluid into the sample injection pipeline 104, and soaking for a first period of time;
s1012, controlling the first power assembly 108 to alternately exhaust and inhale, and cleaning the sample injection pipeline 104;
s1013, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid out of the sample injection pipeline 104;
s1021, controlling the first power component 108 to suck air, and sucking the air into the sample injection pipeline 104;
S1022, controlling the first power assembly 108 to exhaust, and discharging air from the sample needle 1014 into the sheath flow cell 101;
s1023, controlling the first power assembly 108 to suck air, and sucking the cleaning fluid into the sample injection pipeline 104;
s1024, controlling the first power assembly 108 to exhaust, and discharging the cleaning liquid from the sample needle 1014 into the sheath flow cell 101;
s1031, controlling the second power assembly 112 to inhale, sucking the cleaning liquid splashed on the wall of the sheath flow tank 101 into the sheath flow tank 101, and soaking for a second period of time;
s1032, controlling the second power assembly 112 to alternately exhaust and inhale, and cleaning the sheath flow tank 101;
s1041, controlling the second power assembly 112 to exhaust, and discharging the cleaning liquid in the sheath liquid pipeline 110;
s1042, controlling the second power assembly 112 to suck air, and sucking sheath fluid into the second power assembly 112;
s1043, controlling the first power assembly 108 to suck air, and sucking sheath fluid into the first power assembly 108;
s1044, controlling the second power assembly 112 to exhaust, pushing the sheath fluid into the sheath flow cell 101, and simultaneously, the controller 114 adjusts the first valve assembly 107 to the first working position, controlling the first power assembly 108 to exhaust, pushing the sheath fluid into the sheath flow cell 101, and extruding the excess fluid from the waste liquid port 1013.
In the present embodiment, S1041 to S1044 are specific control flows for controlling the first power assembly 108 and the second power assembly 112 to fill the sheath flow tank 101 with sheath fluid in step S104. The steps S1042 and S1043 are not strictly sequential, and the implementation of the cleaning sheath flow cell 101 is not affected by executing S1042 and then S1043, or executing S1043 and then S1042, or executing S1042 and S1043 simultaneously.
In step S1041, the controller 114 controls the second power assembly 112 to exhaust, and discharges the cleaning solution in the sheath fluid line 110, such that no cleaning solution remains on the sheath fluid line 110.
In step S1042, the controller 114 adjusts the second valve assembly 111 to the second operating position so that the second power assembly 112 is in communication with the sheath fluid reservoir 113. The sheath fluid can be sucked into the second power assembly 112 by controlling the suction of the second power assembly 112.
In step S1043, the controller 114 adjusts the first valve assembly 107 to the second working position such that the first power assembly 108 is in communication with the sheath fluid reservoir 113. The sheath fluid may be drawn into the primary power assembly 108 by controlling the suction of the primary power assembly 108.
In step S1044, the controller 114 adjusts the second valve assembly 111 to the first working position, so that the second power assembly 112 is in communication with the sheath fluid line 110. The second power assembly 112 is controlled to exhaust, and the sheath fluid sucked into the second power assembly 112 in step S1042 is pushed out into the sheath flow cell 101. At the same time, the controller 114 adjusts the first valve assembly 107 to the first operating position, so that the first power assembly 108 and the sheath fluid line 110 are in communication. The first power assembly 108 is controlled to exhaust, and the sheath fluid sucked into the first power assembly 108 in step S1043 is pushed out into the sheath flow cell 101.
Since the sheath liquid inlet 1012 is located at the lower portion of the sheath flow cell 101, the sheath liquid entering the sheath flow cell 101 from the sheath liquid inlet 1012 gathers from the bottom of the sheath flow cell 101, and the liquid of the mixed oil washing liquid in the previous step is extruded from the liquid outlet 1013. Sheath fluid entering from the sample fluid inlet 1011 enters the sheath flow cell 101 through the sample needle 1014, and the air in the sample needle 1014 is exhausted, so that the sample needle 1014 is filled with the sheath fluid, and the measurement accuracy in use is ensured.
In some embodiments, the flow sample analyzer further comprises a fault detection module for detecting an abnormal condition of the flow sample analyzer; the controller is also used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow cell through the sample needle when the abnormal condition is related to the existence of bubbles in the flow chamber, and controlling the second power component to alternately execute the action of exhausting and sucking so as to eliminate the bubbles in the sheath flow cell.
Optionally, the controller is further configured to execute the workflow of S101 to S104 provided in the foregoing embodiment when the abnormal situation is related to the existence of bubbles in the flow chamber, so as to eliminate the bubbles in the sheath flow cell, and complete cleaning of the flow chamber. The abnormal conditions related to the existence of bubbles in the flow chamber can be scenes such as flow chamber hole blocking, sheath flow abnormality, abnormal detection result and the like. Compared with the situation that the machine needs to be disassembled for confirmation when faults caused by the optical module, the hardware module and the like exist in the flow chamber, the flow chamber is cleaned and maintained preferentially through the mode, the fault elimination efficiency of the instrument can be improved, and fault positioning is effectively carried out.
The present embodiment provides a flow chamber cleaning method, which is applied to a flow sample analyzer, the flow sample analyzer includes: the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline; the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline; the controller is respectively connected with the first power assembly and the second power assembly;
the flow chamber cleaning method comprises the following steps:
controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharging the cleaning liquid into the sheath flow cell through the sample needle;
controlling the second power assembly to alternately perform an air exhausting and sucking action so as to eliminate air bubbles in the sheath flow cell and discharge the cleaning liquid from the waste liquid port; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount.
In this embodiment, the beneficial effects of the flow chamber cleaning method are identical to those of the flow sample analyzer described above, and will not be described in detail herein.
The present embodiment provides a flow sample analyzer, including:
the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
and the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle, discharging the cleaning liquid into the sheath flow tank through the sample needle, controlling the second power component to suck in the cleaning liquid discharged into the sheath flow tank by the sample needle to soak the sheath flow tank, and discharging the cleaning liquid from the waste liquid outlet by controlling the second power component to exhaust after soaking for a second period of time.
The beneficial effects of the flow sample analyzer provided in this embodiment are identical to those of the flow sample analyzer described above, and will not be described in detail herein.
It is understood that those skilled in the art can combine the various embodiments of the above embodiments to obtain technical solutions of the various embodiments under the teachings of the above embodiments.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (9)
1. A flow sample analyzer, comprising:
the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle, and controlling the second power component to alternately execute the air exhaust and suction actions so as to eliminate air bubbles in the sheath flow tank and discharge the cleaning liquid from the waste liquid outlet; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount;
Wherein the controller is further configured to, before the controller controls the second power assembly to alternately perform the degassing and aspiration actions to eliminate bubbles in the sheath flow cell:
controlling the second power assembly to suck in so as to suck down the cleaning liquid discharged into the sheath flow reservoir by the sample needle to soak the sheath flow reservoir, wherein the second duration of the cleaning liquid soaking the sheath flow reservoir is not longer than the duration of the cleaning liquid diffusing to the second power assembly.
2. The flow sample analyzer of claim 1, wherein the second time period is determined by a length of the sheath fluid line and a cleaning fluid diffusion rate, the second time period satisfying the relationship:
wherein:
t is a second duration;
l is the length of the sheath liquid pipeline;
v is the diffusion rate of the cleaning liquid.
3. The flow sample analyzer of claim 1, wherein the sheath fluid conduit has a volume greater than or equal to 1/2 of the volume of the sheath flow cell.
4. The flow sample analyzer of claim 1, wherein the controller controls the first power assembly to push the cleaning fluid in the sample line to the sample needle prior to further:
Controlling the first power assembly to clean the sample injection pipeline;
the controller is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle, and is also used for:
and controlling the first power assembly and the second power assembly to fill the sheath flow tank with sheath liquid.
5. The flow sample analyzer of claim 4, wherein the controller controlling the first power assembly to purge the sample line comprises:
controlling the first power assembly to inhale, inhaling the cleaning liquid into the sample introduction pipeline, and soaking for a first period of time;
controlling the first power assembly to alternately exhaust and inhale, and cleaning the sample injection pipeline;
controlling the first power assembly to exhaust, and discharging the cleaning liquid out of the sample injection pipeline;
after the controller executes the control that the first power component pushes the cleaning liquid in the sample injection pipeline to the sample needle, the controller is further used for:
controlling the first power assembly to suck air, and sucking the air into the sample injection pipeline;
controlling the first power assembly to vent, venting air from the sample needle into the sheath flow cell;
controlling the first power assembly to suck air, and sucking the cleaning liquid into the sample injection pipeline;
And controlling the first power assembly to exhaust, and discharging the cleaning liquid from the sample needle into the sheath flow cell.
6. The flow sample analyzer of claim 4, wherein controlling the first and second power assemblies such that the sheath flow cell is filled with sheath fluid comprises:
controlling the second power assembly to exhaust, and discharging the cleaning liquid in the sheath liquid pipeline;
controlling the second power assembly to suck air, and sucking sheath fluid into the second power assembly;
controlling the first power assembly to suck air, and sucking sheath fluid into the first power assembly;
and controlling the second power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, adjusting the first valve assembly to be at a first working position by the controller, controlling the first power assembly to exhaust, pushing out sheath liquid into the sheath flow tank, and extruding redundant liquid from a waste liquid port.
7. The flow sample analyzer of any one of claims 1-6, further comprising a fault detection module for detecting an abnormal condition of the flow sample analyzer;
the controller is also used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle when the abnormal condition is related to the existence of bubbles in the flow chamber, and controlling the second power component to alternately execute the action of exhausting and sucking so as to eliminate the bubbles in the sheath flow tank.
8. A flow cell cleaning method for use with a flow sample analyzer, the flow sample analyzer comprising: the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline; the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline; the controller is respectively connected with the first power assembly and the second power assembly; the method for cleaning the flow chamber is characterized by comprising the following steps:
controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharging the cleaning liquid into the sheath flow cell through the sample needle;
controlling the second power assembly to alternately perform an air exhausting and sucking action so as to eliminate air bubbles in the sheath flow cell and discharge the cleaning liquid from the waste liquid port; when the second power assembly alternately executes the exhausting and sucking actions, the exhaust amount is larger than the sucking amount;
wherein prior to the step of controlling the second power assembly to alternately perform an exhaust inhalation to eliminate bubbles in the sheath flow cell, the flow chamber cleaning method further comprises:
Controlling the second power assembly to suck in so as to suck down the cleaning liquid discharged into the sheath flow reservoir by the sample needle to soak the sheath flow reservoir, wherein the second duration of the cleaning liquid soaking the sheath flow reservoir is not longer than the duration of the cleaning liquid diffusing to the second power assembly.
9. A flow sample analyzer, comprising:
the sheath flow tank comprises a sample liquid inlet at the bottom of the sheath flow tank, a sheath liquid inlet arranged on the side wall of the lower part and a waste liquid outlet arranged at the top, wherein a sample needle is arranged in the sheath flow tank, one end of the sample needle is communicated with the sample liquid inlet, and the waste liquid outlet is communicated with a waste liquid pipeline;
the sample liquid inlet is connected with the first power component through a sample injection pipeline, and the sheath liquid inlet is connected with the second power component through a sheath liquid pipeline;
the controller is respectively connected with the first power component and the second power component and is used for controlling the first power component to push the cleaning liquid in the sample injection pipeline to the sample needle and discharge the cleaning liquid into the sheath flow tank through the sample needle, controlling the second power component to suck in so as to suck the cleaning liquid discharged into the sheath flow tank by the sample needle down to soak the sheath flow tank, and controlling the second power component to exhaust after soaking for a second period of time and discharging the cleaning liquid from the waste liquid outlet;
Wherein the second duration of the cleaning solution soaking the sheath flow reservoir is not longer than the duration of the cleaning solution diffusing to the second power assembly.
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| CN116037588A (en) * | 2023-01-17 | 2023-05-02 | 中元汇吉生物技术股份有限公司 | Classification pool cleaning system, method and analyzer |
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2023
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| JPH0511059U (en) * | 1991-07-29 | 1993-02-12 | 日本分光株式会社 | Flow path cleaning device for auto sampler |
| CN102549421A (en) * | 2009-09-07 | 2012-07-04 | 株式会社岛津制作所 | Autosampler |
| CN110031386A (en) * | 2019-05-16 | 2019-07-19 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer liquid channel system and its detection method |
| CN112858659A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sampling needle cleaning system, sample analyzer and immune luminescence analyzer |
| CN116037588A (en) * | 2023-01-17 | 2023-05-02 | 中元汇吉生物技术股份有限公司 | Classification pool cleaning system, method and analyzer |
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