CN112858662A - Blood analyzer and sample analysis system - Google Patents
Blood analyzer and sample analysis system Download PDFInfo
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- CN112858662A CN112858662A CN201911195026.5A CN201911195026A CN112858662A CN 112858662 A CN112858662 A CN 112858662A CN 201911195026 A CN201911195026 A CN 201911195026A CN 112858662 A CN112858662 A CN 112858662A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 96
- 239000008280 blood Substances 0.000 title claims abstract description 96
- 238000004458 analytical method Methods 0.000 title claims abstract description 29
- 238000005070 sampling Methods 0.000 claims abstract description 80
- 238000004159 blood analysis Methods 0.000 claims abstract description 36
- 238000003018 immunoassay Methods 0.000 claims abstract description 29
- 210000000601 blood cell Anatomy 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims description 107
- 239000012530 fluid Substances 0.000 claims description 48
- 239000002699 waste material Substances 0.000 claims description 38
- 238000002347 injection Methods 0.000 claims description 30
- 239000007924 injection Substances 0.000 claims description 30
- 210000003743 erythrocyte Anatomy 0.000 claims description 25
- 238000004820 blood count Methods 0.000 claims description 23
- 238000002156 mixing Methods 0.000 claims description 18
- 210000004027 cell Anatomy 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 6
- 238000005086 pumping Methods 0.000 claims description 4
- 230000004308 accommodation Effects 0.000 claims 1
- 230000002026 carminative effect Effects 0.000 claims 1
- 238000007599 discharging Methods 0.000 claims 1
- 238000013022 venting Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 12
- 238000004140 cleaning Methods 0.000 description 55
- 239000000243 solution Substances 0.000 description 8
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 238000010926 purge Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
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Abstract
The application discloses blood analysis device and sample analysis system, this blood analysis device includes: the blood cell analyzer comprises a sampling needle mechanism, an immunoassay mechanism and a blood cell analysis mechanism, wherein the sampling needle mechanism comprises a sampling needle and a guide rail for driving the sampling needle to move; the immunoassay mechanism is used for receiving the blood sample to be tested distributed by the sampling needle so as to carry out immunoassay; and the blood cell analysis mechanism is used for receiving the blood sample to be tested distributed by the sampling needle so as to carry out blood cell analysis. Through the mode, the application can carry out comprehensive detection to the blood sample that awaits measuring to the detection of blood sample for awaiting measuring facilitates.
Description
Technical Field
The present application relates to the field of medical devices, and in particular, to a blood analysis device and a sample analysis system.
Background
Immunoassay is a method for analyzing blood, and is widely used in the fields of biology and medical treatment because of its high sensitivity.
However, currently, immunoassay does not involve detection of blood cells, and when immunoassay and blood cell analysis are required, separate and independent operations are required, which causes inconvenience in detection of a sample.
Disclosure of Invention
The technical problem that this application mainly solved provides a blood analysis device and sample analysis system, can carry out comprehensive detection to the blood sample that awaits measuring to the detection of blood sample that awaits measuring facilitates.
In order to solve the technical problem, the application adopts a technical scheme that: the blood analysis device comprises a sampling needle mechanism, an immunoassay mechanism and a blood cell analysis mechanism, wherein the sampling needle mechanism comprises a sampling needle and a guide rail for driving the sampling needle to move; the immunoassay mechanism is used for receiving the blood sample to be tested distributed by the sampling needle so as to carry out immunoassay; and the blood cell analysis mechanism is used for receiving the blood sample to be tested distributed by the sampling needle so as to carry out blood cell analysis.
In order to solve the above technical problem, another technical solution adopted by the present application is: there is provided a blood analysis system comprising a blood analysis device as described above.
The beneficial effect of this application is: different from the prior art, the immunoassay mechanism is used for receiving the blood sample to be tested distributed by the sampling needle for immunoassay, and the blood cell analysis mechanism is used for receiving the blood sample to be tested distributed by the sampling needle for blood cell analysis. Through this kind of mode, usable sampling needle once gathers the blood sample that awaits measuring, alright carry out immunoassay and blood cell analysis to the blood sample that awaits measuring simultaneously to realize immunoassay and blood cell analysis integration, thereby can carry out comprehensive detection to the blood sample that awaits measuring, thereby facilitate for the detection of the blood sample that awaits measuring.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a schematic configuration diagram of an embodiment of a blood analyzer according to the present application;
FIG. 2 is a schematic structural view of another embodiment of the blood analysis apparatus of the present application;
FIG. 3 is a schematic configuration diagram of an embodiment of the blood analysis system of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a blood analysis apparatus according to the present application.
In this embodiment, the blood analyzer may include a sampling needle mechanism 11, an immunoassay mechanism 12, and a blood cell analysis mechanism 13, and further, the blood analyzer may further define an automatic sample injection site 10a and an open sample injection site 10 b.
The sampling needle mechanism 11 may include a sampling needle 111 and a guide rail 112 for driving the sampling needle 111 to move; the automatic sample injection site 10a is a site for automatically providing a blood sample to be tested, and the open sample injection site 10b is a site for manually providing the blood sample to be tested by an operator; specifically, the automatic sample injection site 10a, the open sample injection site 10b, the immunoassay mechanism 12 and the blood cell analysis mechanism 13 are all located on the travel position of the sampling needle 111, so that the sampling needle 111 can collect a blood sample to be tested on the automatic sample injection site 10a or the open sample injection site 10b under the driving of the guide rail 112, distribute the blood sample to be tested to the immunoassay mechanism 12 at the immunoassay mechanism 12, and distribute the blood sample to be tested to the blood cell analysis mechanism 13 at the blood cell analysis mechanism 13.
Further, the immunoassay mechanism 12 receives the blood sample to be tested distributed by the sampling needle 111 for immunoassay, and the blood cell analysis mechanism 13 receives the blood sample to be tested distributed by the sampling needle 111 for blood cell analysis. Through this kind of mode, usable sampling needle 111 once gathers the blood sample that awaits measuring, alright carry out immunoassay and blood cell analysis to the blood sample that awaits measuring simultaneously to realize immunoassay and blood cell analysis integration, thereby can carry out comprehensive detection to the blood sample that awaits measuring, thereby facilitate for the detection of the blood sample that awaits measuring.
Specifically, the blood cell analysis mechanism 13 may be at least one of a blood type detection mechanism, a red blood cell counting mechanism, a white blood cell counting mechanism, a platelet counting mechanism, and the like.
In one embodiment, the blood cell analysis mechanism 13 may include a red blood cell counting chamber 131, and the red blood cell counting chamber 131 is used for receiving the blood sample to be tested dispensed by the sampling needle 111 to perform red blood cell counting and volume measurement on the blood sample to be tested.
The red blood cell counting cell 131 can perform red blood cell counting and volume measurement by an impedance method, and specifically, can be disposed between the automatic sample injection site 10a and the open sample injection site 10 b. It should be noted that, the red blood cell counting cell 131 is disposed between the automatic sample injection site 10a and the open sample injection site 10b, so that the interference of mechanical vibration and the influence of electromagnetic interference can be reduced, and the influence of background noise can be reduced.
Specifically, the immunoassay mechanism 12 may include an incubation disc 121, the incubation disc 121 is provided with a corresponding immunoassay sub-site 121a, when a blood sample to be detected is detected, the guide rail 112 drives the sampling needle 111 to reach the automatic sample injection site 10a or the open sample injection site 10b to collect the blood sample to be detected, then the guide rail 112 drives the sampling needle 111 to reach the immunoassay sub-site 121a to distribute the blood sample to be detected used in immunoassay, and after the distribution of the blood sample to be detected is completed, the guide rail 112 further drives the sampling needle 111 to reach the red blood cell counting cell 131 to distribute the blood sample to be detected used in blood cell detection. Further, after the sampling needle 111 dispenses the blood sample to the immunoassay blood dispensing site 121a, the immunoassay mechanism 12 further performs operations such as incubation, magnetic separation, and counting of the blood sample to be measured in cooperation with other relevant components of the blood analysis apparatus; after the sampling needle 111 dispenses the blood sample to be measured into the red blood cell count cell 131, red blood cell count and volume measurement are further performed in cooperation with other relevant components of the blood analysis apparatus.
Referring to fig. 2, the red blood cell counting cell 131 may include a housing chamber 1311, and defines a sample inlet 131a, a liquid injection hole 131b, a liquid discharge hole 131c, a counting hole 131d, a cleaning hole 131e and a cleaning hole 131f, which are communicated with the housing chamber 1311.
The sample inlet 131a and the liquid injection hole 131b may be respectively located at the top of the accommodating chamber 1311, the liquid discharge hole 131c may be located at the bottom of the accommodating chamber 1311, and the counting hole 131d, the cleaning hole 131e, and the cleaning hole 131f may be located on the sidewall of the accommodating chamber 1311.
The sampling needle 111 can reach the position of the sampling hole 131a under the driving of the guide rail 112, and further extend into the accommodating chamber 1311 through the sampling hole 131a, so as to dispense a corresponding blood sample to be tested in the accommodating chamber 1311.
Further, before the blood sample is subjected to red blood cell counting, the blood sample needs to be diluted by sheath fluid because the concentration of the blood sample is high. Specifically, the blood sample to be tested may be diluted in the accommodating chamber 1311 of the red blood cell counting cell 131, and the number of times of dilution may be determined according to the final dilution concentration, the capacity of the accommodating chamber 1311, and other factors. In this embodiment, the sheath fluid can be used to dilute the blood sample to be tested twice.
Specifically, sheath fluid may be injected into the accommodating cavity 1311, and a blood sample to be measured may be further injected, after mixing, a first mixed blood sample is obtained, the sampling needle 111 is used to absorb a preset amount of the first mixed blood sample, and the remaining first mixed blood sample in the accommodating cavity 1311 is discharged, so as to complete first dilution; then, after injecting sheath fluid into the accommodating cavity 1311 for cleaning, further injecting the sheath fluid, adding a preset amount of the first uniform blood sample in the sampling needle 111, and further uniformly mixing to obtain a second uniform blood sample, thereby completing the second dilution.
It should be noted that the sheath fluid used for the first blending, the sheath fluid used for cleaning the accommodating cavity 1311 after the first blending, and the sheath fluid used for the second blending may be injected into the accommodating cavity 1311 in the same manner.
Specifically, the blood analysis apparatus may further include a syringe 21, a valve 301, a valve 302, a valve 303, a sheath fluid port 40a, and a tube connecting the respective components.
The valve 301, the valve 302 and the valve 303 may be three-way valves, the injector 21 is connected to a common end of the valve 301, a first switching end of the valve 301 is connected to the common end of the valve 302, a first switching end of the valve 302 is connected to the sheath fluid port 40a, and a second switching end of the valve 302 is connected to other components, wherein the sheath fluid port 40a is further used to connect a sheath fluid container for containing sheath fluid, further, a second switching end of the valve 301 is connected to the common end of the valve 303, the first switching end of the valve 303 is connected to a fluid inlet hole of the red blood cell counting cell 131, and the second switching end of the valve 303 is connected to other components.
When the sheath liquid needs to be injected into the accommodating cavity 1311, the common end of the valve 301 is communicated with the first switching end, the common end of the valve 302 is communicated with the first switching end, and under the suction action of the syringe 21, the sheath liquid in the sheath liquid container enters the syringe 22 through the sheath liquid interface 40a, the valve 302 and the valve 301; after the injector 22 sucks the sheath fluid, the common end of the valve 301 is further communicated with the second switching end, and the common end of the valve 303 is communicated with the first switching end, so that the sheath fluid sucked by the injector 21 enters the accommodating cavity 1311 through the valve 301, the valve 303 and the injection hole 131b under the pushing action of the injector 21.
It should be noted that when the sheath fluid needs to be injected into the accommodating chamber 1311, if the syringe 21 has enough sheath fluid stored therein, the syringe 21 is directly pushed to inject the sheath fluid into the accommodating chamber 1311 without further sucking the sheath fluid.
Further, in this embodiment, the blood analysis apparatus may further include a syringe 22, an air inlet pump 23, a positive pressure tank 51, an air inlet valve 304, a three-way joint 61, two air filters 24, an air outlet valve 305, a pressure sensor 25, a buffer tank 52, and a pipeline connecting the respective components.
The air inlet valve 304 and the air outlet valve 305 may be two-way valves, the injector 22 is connected to the sampling needle 111, the positive pressure tank 51 is connected to one end of the air inlet valve 304, one end of the air outlet valve 305, and one end of the air inlet pump 23, the other end of the air inlet valve 304 is connected to the buffer pool 52, and is further connected to the drain hole 131c through the buffer pool 52, and the two air filters 24 are connected to the other end of the air inlet pump 23 in parallel through the three-way joint 61 to filter the air entering the air inlet pump 23.
After the sheath fluid is injected into the accommodating cavity 1311, the guide rail 112 drives the sampling needle 111 to the position above the sample injection hole 131a, and further extends into the accommodating cavity 1311 through the sample injection hole 131a to reach a position close to the bottom of the accommodating cavity 1311, and then under the pushing action of the syringe 22, the blood sample to be detected in the sampling needle 111 is pushed into the sheath fluid in the accommodating cavity 1311; then, the air intake pump 23 is turned on to make the pressure of the positive pressure tank 51 reach a preset positive pressure, and the air intake valve 304 is opened, so that air is introduced into the accommodating cavity 1311 through the liquid discharge hole 131c, and the sheath fluid and the blood sample to be tested in the accommodating cavity 1311 are mixed uniformly to obtain a first mixed blood sample.
The pressure value of the preset positive pressure can be set according to the actual situation, and in an application scene, the preset positive pressure can be 30 kpa.
It should be noted that, when the sampling needle 111 dispenses the blood sample to be measured into the accommodating cavity 1311, the blood sample to be measured is located at the bottom of the accommodating cavity 1311, so that air is introduced from the liquid discharge hole 131c located at the bottom of the accommodating cavity 1311 to form bubbles moving upward, and the bubbles can perform a good uniform mixing effect on the blood sample to be measured and the sheath fluid in the accommodating cavity 1311 in the upward movement process.
Further, a pressure sensor 25 may be provided on the positive pressure tank 51 for detecting the pressure in the positive pressure tank 51, and when the pressure sensor 25 detects that the pressure in the positive pressure tank 51 is reduced below the preset positive pressure or the difference between the preset positive pressure and the pressure is greater than a set value, the intake pump 23 is controlled to be turned on to continue to inflate the positive pressure tank 51, so that the pressure in the positive pressure tank 51 is maintained near the preset pressure. Further, when the positive pressure tank 51 needs to be exhausted, both ends of the exhaust valve 305 may be controlled to communicate to exhaust the positive pressure tank 51.
Further, the blood analysis apparatus further includes a waste valve 306, a three-way joint 62, a three-way joint 63, a liquid pump 26, a waste port 40b, and a pipeline connecting the respective components.
Specifically, the waste liquid valve 306 may be a two-way valve, the buffer tank 52 is further connected to one end of the waste liquid valve 306, the other end of the waste liquid valve 306 is connected to one end of the liquid pump 26 through the three-way joint 62 and the three-way joint 63, the other end of the liquid pump 26 is further connected to a waste liquid interface 40b, and the waste liquid interface 40b is used for connecting a waste liquid container for containing waste liquid. It should be noted that the three-way joints 62 and 63 may be further connected to other components hereinafter.
After the first homogenized blood sample is obtained, the guide rail 112 drives the sampling needle 111 to extend into the accommodating cavity 1311 through the sample inlet 131a, and a preset amount of the first homogenized blood sample is sucked from the accommodating cavity 1311 under the suction action of the syringe 22; the amount of the first mixed blood sample sucked by the sampling needle 111 can be determined according to the concentration to be diluted, and the like, and is not limited specifically here.
Further, after the sampling needle 111 sucks a preset amount of the first blood sample, the two ends of the waste liquid valve 306 are further communicated, and the liquid pump 26 is opened, so that the remaining first blood sample in the accommodating cavity 1311 is discharged into the buffer pool 52 through the liquid discharge hole 131c, and further discharged into the waste liquid container through the waste liquid valve 306, the three-way joint 62, the three-way joint 63, the liquid pump 26 and the waste liquid interface 40 b.
It should be noted that after the remaining first homogenized blood sample is discharged and further sheath fluid is injected into the accommodating cavity 1311 for cleaning, waste fluid generated by cleaning can still be discharged from the fluid discharge hole 131c in the above manner to complete the first dilution of the blood sample to be measured.
After the chamber 1311 is cleaned, the blood sample to be tested is diluted a second time in the chamber 1311. Specifically, sheath liquid is further injected into the accommodating cavity 1311, and after the sheath liquid is injected, the guide rail 112 drives the sampling needle 111 to enter the accommodating cavity 1311 through the sample injection hole 131a and extend to the bottom position of the accommodating cavity 1311, and under the pushing action of the syringe 22, a preset amount of the first uniform blood sample sucked by the sampling needle 111 is released into the sheath liquid in the accommodating cavity 1311; further, air is introduced from the bottom of the accommodating cavity 1311 through the liquid discharge hole 131c under the cooperation of the air inlet pump 23, the positive pressure tank 51, the air inlet valve 304 and the like to uniformly mix the sheath liquid and the first blood sample, so that a second blood sample is obtained to complete the second dilution of the blood sample to be measured.
Further, the blood analysis apparatus may further include a count valve 307, a negative pressure tank 53, an exhaust valve 308, a suction valve 309, and a pipe connecting the respective components.
The counting valve 307, the exhaust valve 308, and the exhaust valve 309 may be three-way valves, two ends of the counting valve 307 are respectively connected to the counting hole 131d and the negative pressure tank 53, the negative pressure tank 53 is further respectively connected to one end of the exhaust valve 308 and one end of the exhaust valve 309, and the exhaust valve 309 and the waste liquid valve 306 are connected to the liquid pump 26 through the three-way joint 62.
After the second mixed blood sample is obtained, the two ends of the counting valve 307 and the two ends of the air extraction valve 309 are respectively communicated, and the liquid extraction pump 26 is opened to make the pressure of the negative pressure tank 53 reach a preset negative pressure, so that the second mixed blood sample enters the counting hole 131d to perform red blood cell counting and volume measurement; further, the counted second mixed blood sample is discharged to the negative pressure tank 53 through the counting valve 307, and enters the waste liquid container through the suction valve 309, the three-way joint 62, the three-way joint 63, the liquid pump 26 and the waste liquid port 40b under the suction action of the liquid pump 26.
The pressure value of the preset negative pressure required to be reached by the negative pressure tank 53 may be a specific pressure value, or may be a pressure value range, and may be specifically set according to actual requirements. In addition, the exhaust valve 308 is used to exhaust the negative pressure tank 53 by communicating both ends when the negative pressure tank 53 needs to exhaust gas.
Further, the blood analysis apparatus may further include a three-way joint 64, a purge valve 310, and a pipeline connecting the components, wherein the purge valve 310 may be a two-way valve, the purge hole 131e and the purge hole 131f are respectively connected to one end of the purge valve 310 through the three-way joint 64, and the other end of the purge valve 310 is connected to the negative pressure tank 53.
After the second homogenized blood sample is discharged, the sheath fluid is injected into the accommodating chamber 1311 through the injection hole 131b, in the same manner as the above-described method for injecting the sheath fluid into the accommodating chamber 1311. After the sheath fluid is injected, the two ends of the cleaning valve 310 and the two ends of the suction valve 309 are respectively communicated, the counting valve 307 is disconnected, the liquid-pumping pump 26 is opened, so that the sheath fluid in the accommodating cavity 1311 respectively enters the negative pressure tank 53 through the cleaning hole 131e, the cleaning hole 131f, the three-way joint 64 and the cleaning valve 310, and enters the waste liquid container through the waste liquid interface 40b under the suction action of the liquid-pumping pump 26, thereby completing the cleaning of the erythrocyte counting cell 131.
Further, the blood analysis apparatus may further include a strong washing solution container 54, a valve 311, a strong washing reservoir 55, an outlet valve 312, and a pipe connecting the respective components. Wherein, the valve 311 can be a three-way valve, and the liquid outlet valve 312 can be a two-way valve; the powerful cleaning tank 55 includes a cleaning chamber 551, and defines a cleaning port 55a, a liquid inlet 55b and a liquid outlet 55c respectively communicating with the cleaning chamber 551. The second switching end of the valve 302 is connected to the common end of the valve 311, the first switching end of the valve 311 is connected to the powerful cleaning liquid container 54, the second switching end of the valve 311 is connected to the liquid inlet 55b of the powerful cleaning tank 55, and one end of the liquid outlet valve 312 is connected to the liquid outlet 55c of the powerful cleaning tank 55.
In this embodiment, after the sampling needle 111 releases the first homogenized blood sample into the accommodating chamber 1311 of the erythrocyte counting chamber 131, the inner wall and the outer wall of the sampling needle 111 may be further cleaned. Since the inner wall of the sampling needle 111 directly contacts the blood sample, proteins in the blood sample and the like are adsorbed on the inner wall of the sampling needle 111, so that the inner wall of the sampling needle 111 is not easily cleaned.
In this embodiment, when cleaning the inner wall of the sampling needle 111, a strong cleaning solution is first used to perform strong cleaning, wherein the strong cleaning solution may be a strong alkaline cleaning solution or a strong acidic cleaning solution, and can dissolve proteins and the like attached to the inner wall of the sampling needle 111.
Specifically, when the inner side wall of the sampling needle 111 needs to be strongly cleaned, the injector 21 may first suck a certain amount of sheath fluid, then communicate the common end of the valve 302 with the second switching end, communicate the common end of the valve 311 with the first switching end, suck the strong cleaning fluid in the strong cleaning fluid container 54 between the valve 302 and the valve 311 under the suction action of the injector 21, further communicate the common end of the valve 311 with the second switching end, push the strong cleaning fluid between the valve 302 and the valve 311 into the strong cleaning fluid pool by the sheath fluid in the injector 21 under the pushing action of the injector 21, then the guide rail 112 drives the sampling needle 111 to enter the cleaning cavity 551 from the cleaning port 55a, and sucks the strong cleaning fluid under the suction action of the injector 22, and push out the strong cleaning fluid sucked by the sampling needle 111 under the pushing action of the injector 22, thereby cleaning the inner wall of the sampling needle 111 with a strong cleaning liquid.
It should be noted that, when the inner wall of the sampling needle 111 is strongly cleaned, if a strong cleaning liquid is already present in the strong cleaning reservoir 55, it is not necessary to inject the strong cleaning liquid into the strong cleaning liquid again by using the syringe 21. In addition, after the powerful cleaning liquid in the powerful cleaning tank 55 cleans the inner wall of the sampling needle 111 once or more times, and the cleaning force is not enough to continuously and forcefully clean the inner wall of the sampling needle 111, the liquid outlet valve 312 may be opened to discharge the powerful cleaning liquid therein.
Because the powerful cleaning liquid has a certain corrosivity, the valve 311 through which the powerful cleaning liquid needs to pass can be made of an anti-corrosion material, and meanwhile, the pipeline between the valve 302 and the valve 311 is long enough, so that the injector 21 can prevent the powerful cleaning liquid from entering the valve 302 to corrode the valve 302 when sucking the powerful cleaning liquid.
Further, after the inner side wall of the sampling needle 111 is strongly cleaned, the inner side wall of the sampling needle 111 can be further normally cleaned by using sheath fluid, so as to further clean the strong cleaning fluid remained on the inner side wall of the sampling needle 111; the outer sidewall of the sampling needle 111 may then be further cleaned.
Specifically, the blood analysis apparatus may further include a valve 313, a valve 314, a washing swab 71 disposed on the periphery of the sampling needle 111, and a pipeline connecting the components, wherein the valve 313 may be a three-way valve, the valve 314 may be a two-way valve, the washing swab 71 defines an inlet 71a and an outlet 71b, the second switching end of the valve 303 is connected to the common end of the valve 313, the first switching end of the valve 313 is connected to the syringe 22, the second switching end of the valve 313 is connected to the inlet 71a of the washing swab 71, one end of the valve 314 is connected to the outlet 71b of the washing swab 71, and the other end of the valve 314 is connected between the three-way joint 62 and the liquid pump 26 through the three-way joint 63.
When the inner wall of the sampling needle 111 is normally cleaned, a certain amount of the sheath fluid is sucked by the syringe 21, but if the sheath fluid is sufficiently sucked by the syringe 21, further suction may not be necessary. Then, the common end of the valve 301 is communicated with the second switching end, the common end of the valve 303 is communicated with the second switching end, and the common end of the valve 313 is communicated with the first switching end, so that the sheath fluid sucked by the injector 21 is pushed into the sampling needle 111 through the valve 301, the valve 303, the valve 313 and the injector 22 under the pushing action of the injector 21, so as to perform ordinary cleaning on the inner side wall of the sampling needle 111.
When the outer wall of the sampling needle 111 is cleaned, a certain amount of the sheath fluid is sucked by the syringe 21, and similarly, if a sufficient amount of the sheath fluid is sucked by the syringe 21, further suction is not necessary. Then, the common end of the valve 303 is communicated with the second switching end, the common end of the valve 313 is communicated with the second switching end, the two ends of the valve 314 are communicated, the liquid pump 26 is turned on, the sheath liquid sucked by the injector 21 is pushed into the cleaning swab 71 through the valve 303, the valve 313 and the inlet 71a of the cleaning swab 71 under the pushing action of the injector 21 to clean the outer wall of the sampling needle 111, and the waste liquid generated by cleaning is pumped out into the waste liquid container through the outlet 71b of the cleaning swab 71, the valve 314, the three-way joint 63, the liquid pump 26 and the waste liquid interface 40b under the pumping action of the liquid pump 26, so that the cleaning of the sampling needle 111 is completed.
Referring to fig. 3, fig. 3 is a schematic structural diagram of an embodiment of the blood analysis system of the present application. In this embodiment, the blood analysis system 1000 includes the blood analysis device 100, and the structure and function of the blood analysis device 100 are the same as those of the blood analysis device of the present application, and the detailed description thereof is omitted herein for reference.
The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.
Claims (10)
1. A blood analysis apparatus, comprising:
the sampling needle mechanism comprises a sampling needle and a guide rail for driving the sampling needle to move;
the immunoassay mechanism is used for receiving the blood sample to be detected distributed by the sampling needle so as to carry out immunoassay;
and the blood cell analysis mechanism is used for receiving the blood sample to be tested distributed by the sampling needle so as to carry out blood cell analysis.
2. The blood analysis device of claim 1, wherein the blood cell analysis mechanism comprises a red blood cell counting chamber for receiving the blood sample to be tested dispensed by the sampling needle for red blood cell counting and volume measurement;
the blood analysis device is defined with an automatic sample injection position and an open sample injection position for the sampling needle to collect a blood sample to be detected, and the red blood cell counting cell is arranged between the automatic sample injection position and the open sample injection position.
3. The blood analysis device of claim 2, wherein the red blood cell count cell comprises a housing chamber and defines a drain hole communicating with the housing chamber, the blood analysis device further comprises an air intake pump, a positive pressure tank, and an air intake valve, wherein the positive pressure tank is connected to one end of the air intake valve and the air intake pump, respectively, and the other end of the air intake valve is connected to the drain hole;
wherein, to behind the sheath liquid of holding intracavity injection and the distribution blood sample that awaits measuring, the pump that admits air opens so that the pressure of malleation jar reaches and predetermines the malleation, and opens admit air valve, thereby pass through the flowing back hole to the holding intracavity lets in gas, in order to incite somebody to action the sheath liquid of holding intracavity and the blood sample mixing that awaits measuring obtain first mixing blood sample.
4. The blood analysis device of claim 3, further comprising:
a first three-way joint;
two air filters connected in parallel to the intake pump through the first tee joint to filter the gas entering the intake pump;
first exhaust valve, one end is connected the malleation jar is used for when both ends intercommunication right the malleation jar is carminative.
5. The apparatus according to claim 3, further comprising a pressure sensor disposed on the positive pressure tank for detecting a pressure in the positive pressure tank, wherein when the pressure sensor detects that the pressure in the positive pressure tank is less than the predetermined positive pressure, the air intake pump is controlled to be turned on to fill the positive pressure tank with air so as to maintain the pressure in the positive pressure tank within a predetermined range.
6. The blood analysis device of claim 3, wherein the red blood cell count chamber further defines a sample inlet communicating with the receiving chamber, the blood analysis device further comprises a first syringe connected to the sampling needle, a buffer chamber, a waste liquid valve, a liquid pump, and a waste liquid port, wherein the buffer chamber is connected to the liquid outlet, one end of the air inlet valve, and one end of the waste liquid valve, respectively, the other end of the waste liquid valve is connected to the liquid pump, the liquid pump is further connected to the waste liquid port, and the waste liquid port is used for connecting to a waste liquid container;
after obtaining a first mixing blood sample, the guide rail drives the sampling needle to extend into the accommodating cavity through the sample inlet, and the sampling needle sucks a preset amount of the first mixing blood sample in the accommodating cavity under the suction action of the first injector, then the two ends of the waste liquid valve are communicated, and the liquid pump is opened, so that the first mixing blood sample remaining in the accommodating cavity is discharged into the buffer pool through the liquid discharge hole, and is further discharged into the waste liquid container through the waste liquid valve, the liquid pump and the waste liquid interface.
7. The blood analysis device of claim 6, further comprising a second syringe, a first valve, a sheath fluid port for connection to a sheath fluid container;
the erythrocyte counting chamber is defined with a liquid injection hole communicated with the accommodating cavity, the second injector is connected with the common end of the first valve, the sheath liquid interface is connected with the first switching end of the first valve, and the liquid injection hole is connected with the second switching end of the first valve;
when sheath liquid needs to be injected into the accommodating cavity, the common end of the first valve is communicated with the first switching end, the sheath liquid in the sheath liquid container enters the first injector through the sheath liquid interface under the suction action of the second injector, the common end of the first valve is further communicated with the second switching end, and the sheath liquid sucked by the second injector enters the accommodating cavity through the liquid injection hole under the pushing action of the second injector.
8. The blood analysis apparatus according to claim 7,
discharging the remaining first mixing blood sample to back in the waste liquid container, to the sheath liquid is injected into to the holding intracavity, in order right the holding chamber washs, and with the waste liquid after wasing certainly the outage discharges the back, further to the sheath liquid is injected into to the holding intracavity, the guide rail drive the sampling needle passes through advance the appearance hole and get into the holding intracavity to with the first mixing blood sample injection of predetermined volume the holding intracavity, in order with the sheath liquid mixing in the holding intracavity and obtain the second mixing blood sample.
9. The apparatus according to claim 8, wherein the red blood cell count cell defines a count hole communicating with the accommodation chamber, and further comprising a count valve, a negative pressure tank, a second vent valve, an air suction valve and a second three-way joint, wherein two ends of the count valve are respectively connected to the count hole and the negative pressure tank, the negative pressure tank is further respectively connected to one end of the second vent valve and one end of the air suction valve, the air suction valve and the waste liquid valve are connected to the liquid pump through the second three-way joint, and the second vent valve is used for venting the negative pressure tank when the two ends are communicated;
wherein, after obtaining the second mixing blood sample, will the both ends of count valve the both ends of bleeder valve communicate respectively, and open the drawing liquid pump, so that the pressure of negative pressure jar reaches and predetermines the negative pressure, and then make the second mixing blood sample get into the count hole is in order to carry out red blood cell count and volume measurement, and the second mixing blood sample after the count passes through the count valve and discharges extremely the negative pressure jar, and the pumping action of drawing liquid pump is in the warp waste liquid interface and get into in the waste liquid container.
10. A blood analysis system, characterized in that it comprises a blood analysis device according to any one of claims 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911195026.5A CN112858662A (en) | 2019-11-28 | 2019-11-28 | Blood analyzer and sample analysis system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911195026.5A CN112858662A (en) | 2019-11-28 | 2019-11-28 | Blood analyzer and sample analysis system |
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| CN112858662A true CN112858662A (en) | 2021-05-28 |
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| CN103994962A (en) * | 2014-04-25 | 2014-08-20 | 深圳市帝迈生物技术有限公司 | Fluid system of flow cytometer and flow cytometric detection method |
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| CN207472891U (en) * | 2018-02-08 | 2018-06-08 | 深圳赛斯鹏芯生物技术有限公司 | Blood analyser |
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| CN103994962A (en) * | 2014-04-25 | 2014-08-20 | 深圳市帝迈生物技术有限公司 | Fluid system of flow cytometer and flow cytometric detection method |
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Application publication date: 20210528 |