CN116855351A - Multichannel injection pump liquid separating device for organoid culture - Google Patents

Multichannel injection pump liquid separating device for organoid culture Download PDF

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Publication number
CN116855351A
CN116855351A CN202310992594.8A CN202310992594A CN116855351A CN 116855351 A CN116855351 A CN 116855351A CN 202310992594 A CN202310992594 A CN 202310992594A CN 116855351 A CN116855351 A CN 116855351A
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liquid
module
liquid storage
electromagnetic valve
culture
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李昕泽
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Shaanxi Zeyan Biological Information Technology Co ltd
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Shaanxi Zeyan Biological Information Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/40—Manifolds; Distribution pieces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Molecular Biology (AREA)
  • Computer Hardware Design (AREA)
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Abstract

The invention discloses a multichannel injection pump liquid distribution device for organoid culture, which comprises a base module, a liquid distribution module, a liquid transfer device module and a liquid storage module, wherein the liquid distribution module, the liquid transfer device module and the liquid storage module are fixed on the base module; the stepping motor of the piston pushing table in the liquid transfer device module can accurately control the speed through the controller, so that the requirement of accurate cell culture can be met, and the culture yield is increased; the high-precision injectors of the pipettor module are arranged in square symmetry, so that the space utilization rate is improved, the scientificity and stability of liquid flow regulation are ensured, and the culture production efficiency is improved; the high-precision injector of the liquid-transfering device module and the liquid-storing column body of the liquid-storing module can be replaced, so that the liquid-transfering device can be adapted to various culture liquids, the cost is reduced, and the contrast experiment is facilitated.

Description

Multichannel injection pump liquid separating device for organoid culture
Technical Field
The invention relates to the technical field of organoid culture, in particular to a multichannel injection pump liquid separating device for organoid culture.
Background
Organoids refer to cell clusters formed from cells having stem cell potential after culture by in vitro 3D cell culture techniques. The organoids have great potential in the medical field, not only can be subjected to long-term subculture, but also have more stable phenotype and genetic characteristics than two-dimensional culture products, and have prominent advantages in simulating the occurrence process of organ tissues and physiological and pathological states. The physicochemical properties required for 3D cell culture are very stringent due to the need to maintain the normal physiological conditions required for cell survival and growth. In the culture process, the injection of nutrient components is particularly required to be paid attention, and sometimes, several components are simultaneously injected according to different proportions, which has increasingly strict requirements on the performance of an injection system.
The existing injection pump has low injection precision and unstable injection flow, and a plurality of multi-channel injection pumps are required to be equipped according to the change of injection liquid, so that the working efficiency and the economic cost are very huge.
Disclosure of Invention
In order to overcome the problems of the prior art, the invention aims to provide a multichannel injection pump liquid separating device for organoid culture, which provides a standardized, self-controllable, high-efficiency and high-stability liquid separating device for organoid culture.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a multichannel injection pump liquid distribution device for organoid culture comprises a base module 1, a liquid distribution module 2, a liquid shifter module 3 and a liquid storage module 4, wherein the liquid distribution module 2, the liquid shifter module 3 and the liquid storage module 4 are fixed on the base module 1;
the base module 1 is positioned at the bottommost part and can rotate; comprises a bottom disc 11, a rotating shaft 13 and a stepping motor 12; the bottom disc 11 is positioned at the lowest part and can rotate; the stepping motor 12 is positioned at the center position above the bottom disc 11 and is driven by the controller; the rotating shaft 13 is connected above the stepping motor 12 and provided with threads, and is controlled to rotate by the stepping motor 12;
the liquid separation module 2 is fixed on the base module 1 and comprises a guide rod 21, a top end bracket 22, an electromagnetic valve 23, an electromagnetic valve external interface 24-1, an electromagnetic valve internal interface 24-2 and an electromagnetic valve lower interface 24-3; the guide rods 21 are symmetrically fixed on the bottom disc 11, and the upper part of the guide rods is connected with the top end bracket 22; the top end support 22 is of a hollow structure; the electromagnetic valves 23 are positioned in the top end support 22, horizontally penetrate the top end support 22 from outside to inside, the electromagnetic valve external interface 24-1 is connected with the electromagnetic valves 23 and external cell culture components, and positioned outside the top end support 22, the electromagnetic valve internal interface 24-2 is connected with the electromagnetic valves 23 and the liquid storage column 41 of the liquid storage module 4, positioned inside the top end support 22, the electromagnetic valve lower interface 24-3 is connected with the electromagnetic valves 23 and the high-precision injector 32 of the liquid transfer module 3, and positioned at the lower side of the top end support 22; the controller controls the solenoid valve external interface 24-1, the solenoid valve internal interface 24-2 and the solenoid valve lower interface 24-3 to be opened and closed, so that the cell culture liquid is controlled to enter the high-precision syringe 32 from the liquid storage column 41 or enter the external cell culture component from the high-precision syringe 32 for cell culture.
The liquid dispenser module 3 is connected between the base module 1 and the liquid separation module 2 through a rotating shaft 13, and the liquid dispenser module 3 comprises a piston pushing table 31 and a high-precision injector 32; the piston pushing table 31 passes through the rotating shaft 13 and the guide rod 21 through corresponding nuts thereon, and the piston pushing table 31 can reciprocate under the control of a stepping motor and a driver; the lower part of the high-precision injector 32 is connected with a piston pushing table 31 through a piston rod, and the upper part of the high-precision injector is communicated with an interface 24-3 below the electromagnetic valve of the liquid separation module 2;
the liquid storage module 4 is positioned between the liquid separation module 2 and the liquid transfer device module 3 and comprises a liquid storage column 41, the upper part of the liquid storage column 41 is connected with an electromagnetic valve internal interface 24-2 in the liquid separation module 2, and the internal liquid is supplemented from the outside and is used for sucking the high-precision syringe 32;
filling cell culture solution into the liquid storage column 41 for standby, and when the multichannel injection pump liquid distribution device works: the controller controls the stepping motor to enable the piston pushing table 31 to move downwards firstly, and controls the electromagnetic valve internal interface 24-2 connected with the liquid storage column 41 and the electromagnetic valve lower interface 24-3 connected with the high-precision injector 32 to be simultaneously opened, so that the liquid storage column 41 connected with the electromagnetic valve 23 is communicated with the high-precision injector 32, and at the moment, cell culture liquid enters the high-precision injector 32 from the liquid storage column 41; when injection and liquid separation are required, the controller controls the stepping motor to enable the piston pushing table 31 to move upwards, and controls the solenoid valve lower interface 24-3 connected with the high-precision injector 32 and the solenoid valve external interface 24-1 connected with the outside to be opened, so that the high-precision injector 32 connected with the solenoid valve 23 is communicated with the outside, and cell culture liquid enters the corresponding cell culture part from the high-precision injector 32 to be used for cell culture.
The stepper motor 12 can precisely control the speed, the repeatability error is three thousandths, and a pulse type input is adopted to generate a fluctuation type flow scheme so as to meet the requirement of precise cell culture and approach to the flow scheme in the human body;
the high-precision injectors 32 are arranged symmetrically in square, the cylinders can be replaced, the cylinders with different sizes meet different injection flow requirements, and the cylinders with different materials are suitable for nutrient solutions for various organoids.
The liquid storage column 41 can be replaced by different materials so as to be suitable for various types of culture solutions, and a single liquid can be added for batch experiments, so that the efficiency is improved, or different types of culture solutions can be added for comparison experiments.
The bottom disc 11 of the base module 1 is provided with support plates 14 symmetrically distributed around the stepper motor 12 for the piston pushing table 31.
The liquid storage module 4 further comprises a fixing bracket 42 and a liquid storage supporting plate 43, wherein the fixing bracket 42 is positioned between the liquid storage columns 41 and is used for fixing the positions of the liquid storage columns 41 to keep stable; a liquid storage support plate 43 is located below the liquid storage column 41 for supporting the liquid storage column 41.
The cover plate 25 is also included, and the size of the cover plate 25 is consistent with that of the hollow part of the top end bracket 22, so that the hollow part can be tightly covered.
The multichannel injection pump liquid separating device for the organoid culture is applied to organoid culture; the full automation is realized through the controller, and the high-yield culture of unified standards can be realized; the pollution risk of the culture medium for manual liquid separation is reduced through full-automatic liquid separation;
compared with the prior art, the invention has the following advantages:
1) The structure of the invention has better stability and higher space utilization rate, and can realize the simultaneous operation of a plurality of high-precision injectors, thereby ensuring the scientificity and stability of liquid flow regulation and improving the cell culture yield;
2) The invention can generate a fluctuation type flow scheme, more accords with the demand of organoid cell culture, is close to the flow mode in the human body, and increases the yield of cell culture;
3) The invention can realize full-automatic control through the controller, accurately control the flow rate and the flow velocity, ensure the adding requirement of the cell culture solution and realize unified high-yield culture.
4) The injector and the column body of the liquid storage column can be replaced, can be used for injecting culture liquids with different nutrient components at the same time, is convenient for comparison experiments, and greatly reduces the production cost.
5) The 16 square symmetrical injectors have higher stability and higher space utilization rate, and can also ensure the stability of flow.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a multi-channel syringe pump liquid-separating device of the invention.
Fig. 2 is a schematic view of a base module structure.
Fig. 3 is a schematic diagram of a liquid separation module structure.
Fig. 4 is a schematic view of a pipette module configuration.
Fig. 5 is a schematic diagram of a liquid storage module structure.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1, the multichannel syringe pump liquid distribution device for organoid culture of the invention comprises a base module 1, a liquid distribution module 2 fixed on the base module 1, a liquid shifter module 3 and a liquid storage module 4.
As shown in fig. 1 and 2, the base module 1 is located at the bottommost part and is rotatable; comprises a bottom disc 11, a rotating shaft 13 and a stepping motor 12; the bottom disc 11 is positioned at the lowest part, and can rotate, so that the operation is convenient; the stepping motor 12 is positioned at the center position above the bottom disc 11 and is driven by the controller; the rotating shaft 13 is connected above the stepping motor 12 and provided with threads, and is controlled to rotate by the stepping motor 12; the supporting plate 14 is connected to the bottom disc 11 and symmetrically distributed around the stepper motor 12.
As shown in fig. 1 and 3, the liquid separation module 2 is fixed on the base module 1 and comprises a guide rod 21, a top end bracket 22, an electromagnetic valve 23, an electromagnetic valve external interface 24-1, an electromagnetic valve internal interface 24-2 and an electromagnetic valve lower interface 24-3; four guide rods 21 are symmetrically fixed on the bottom disc 11, and the upper part of the guide rods is connected with a top end bracket 22; the top end support 22 is of a hollow structure; the electromagnetic valves 23 are positioned in the top end support 22, horizontally penetrate the top end support 22 from outside to inside, four sides of the electromagnetic valves are sixteen in number, the electromagnetic valve external interfaces 24-1 are connected with the electromagnetic valves 23 and external cell culture components, the electromagnetic valve internal interfaces 24-2 are positioned outside the top end support 22, the electromagnetic valves 23 are connected with the liquid storage column 41 of the liquid storage module 4, the electromagnetic valves are positioned inside the top end support 22, the electromagnetic valve lower interfaces 24-3 are connected with the electromagnetic valves 23 and the high-precision injectors 32 of the liquid transfer device module 3, and the electromagnetic valves are positioned at the lower side of the top end support 22; the controller controls the solenoid valve external interface 24-1, the solenoid valve internal interface 24-2 and the solenoid valve lower interface 24-3 to be opened and closed, so that the cell culture liquid is controlled to enter the high-precision injector 32 from the liquid storage column 41 or enter the corresponding cell culture part from the high-precision injector 32 to be used for cell culture. The number of the electromagnetic valve external interfaces 24-1, the electromagnetic valve internal interfaces 24-2 and the electromagnetic valve lower interfaces 24-3 is sixteen respectively, and corresponds to the number of the electromagnetic valves 23. The cover plate 25 is also included, and the size of the cover plate 25 is consistent with that of the hollow part in the middle of the top end bracket 22, so that the hollow part can be tightly covered.
As shown in fig. 1 and 4, the pipette module 3 is connected between the base module 1 and the liquid separation module 2 through a rotation shaft 13, and the pipette module 3 comprises a piston pushing table 31 and a high-precision syringe 32; the piston pushing table 31 passes through the rotating shaft 13 and the guide rod 21 through corresponding nuts thereon, and the piston pushing table 31 can reciprocate under the control of a stepping motor and a driver; the lower part of the high-precision injector 32 is connected with the piston pushing table 31 through a piston rod, and the upper part of the high-precision injector is communicated with the interface 24-3 below the electromagnetic valve of the liquid separation module 2.
As shown in fig. 1 and 5, the liquid storage module 4 is located between the liquid separation module 2 and the liquid transfer module 3, and comprises a liquid storage column 41, a fixed bracket 42 and a liquid storage support plate 43, wherein the liquid storage column 41 is cylindrical, the upper part of the liquid storage column is connected with an electromagnetic valve internal interface 24-2 in the liquid separation module 2, and the lower part of the liquid storage column is connected with the liquid storage support plate 43, and the internal liquid of the liquid storage support plate is supplemented from the outside and is used for sucking the high-precision syringe 32; a fixing bracket 42 is located between the liquid storage columns 41 for keeping the fixing position stable.
As a preferred embodiment of the present invention, the stepper motor 12 can precisely control the speed with a repeatability error of three thousandths, and a pulse input is used to generate a wave-type flow scheme to achieve the accurate cell culture requirement, approaching the flow scheme in the human body.
As the preferred embodiment of the invention, the high-precision injectors 32 are arranged symmetrically in a square shape, the cylinders can be replaced, the cylinders with different sizes meet different injection flow requirements, and the cylinders with different materials are suitable for nutrient solutions for various organoids.
As a preferred embodiment of the present invention, the liquid storage column 41 can be replaced with different materials to be suitable for various types of culture solutions, a single liquid can be added for batch experiments, efficiency can be improved, or different types of culture solutions can be added for performing control experiments.
The working method of the multichannel injection pump liquid separating device for organoid culture comprises the following steps: filling cell culture solution into the liquid storage column 41 for standby, and when the multichannel injection pump liquid distribution device works: the controller controls the stepping motor to enable the piston pushing table 31 to move downwards firstly, and controls the electromagnetic valve internal interface 24-2 connected with the liquid storage column 41 and the electromagnetic valve lower interface 24-3 connected with the high-precision injector 32 to be simultaneously opened, so that the liquid storage column 41 connected with the electromagnetic valve 23 is communicated with the high-precision injector 32, and at the moment, cell culture liquid enters the high-precision injector 32 from the liquid storage column 41; when injection and liquid separation are required, the controller controls the stepping motor to enable the piston pushing table 31 to move upwards, and controls the solenoid valve lower interface 24-3 connected with the high-precision injector 32 and the solenoid valve external interface 24-1 connected with the outside to be opened, so that the high-precision injector 32 connected with the solenoid valve 23 is communicated with the outside, and cell culture liquid enters the corresponding cell culture part from the high-precision injector 32 to be used for cell culture.

Claims (7)

1. A multichannel syringe pump divides liquid device for organoid is cultivateed, its characterized in that: the device comprises a base module (1), a liquid distribution module (2), a liquid transfer device module (3) and a liquid storage module (4) which are fixed on the base module (1);
the base module (1) is positioned at the bottommost part and can rotate; comprises a bottom disc (11), a rotating shaft (13) and a stepping motor (12); the bottom disc (11) is positioned at the lowest part and can rotate; the stepping motor (12) is positioned at the center position above the bottom disc (11) and is driven by the controller; the rotating shaft (13) is connected above the stepping motor (12) and provided with threads, and the stepping motor (12) controls rotation;
the liquid separation module (2) is fixed on the base module (1) and comprises a guide rod (21), a top end bracket (22), an electromagnetic valve (23), an electromagnetic valve external interface (24-1), an electromagnetic valve internal interface (24-2) and an electromagnetic valve lower interface (24-3); the guide rods (21) are symmetrically fixed on the bottom disc (11), and the upper part of the guide rods is connected with the top end bracket (22); the top end bracket (22) is of a hollow structure; the electromagnetic valves (23) are positioned in the top end support (22), the top end support (22) is horizontally penetrated from outside to inside, an electromagnetic valve external interface (24-1) is connected with the electromagnetic valves (23) and external cell culture components, the electromagnetic valve internal interface (24-2) is positioned outside the top end support (22), the electromagnetic valves (23) are connected with a liquid storage column (41) of the liquid storage module (4), the electromagnetic valves are positioned inside the top end support (22), and an electromagnetic valve lower interface (24-3) is connected with a high-precision injector (32) of the electromagnetic valves (23) and the liquid shifter module (3) and positioned at the lower side of the top end support (22); the electromagnetic valve external interface (24-1), the electromagnetic valve internal interface (24-2) and the electromagnetic valve lower interface (24-3) are controlled to be opened and closed by the controller, so that the cell culture liquid is controlled to enter the high-precision injector (32) from the liquid storage column (41) or enter the external cell culture component from the high-precision injector (32) for cell culture.
The liquid dispenser module (3) is connected between the base module (1) and the liquid separation module (2) through a rotating shaft (13), and the liquid dispenser module (3) comprises a piston pushing table (31) and a high-precision injector (32); the piston pushing table (31) passes through the rotating shaft (13) and the guide rod (21) through corresponding nuts on the piston pushing table, and the piston pushing table (31) can reciprocate under the control of a stepping motor and a driver; the lower part of the high-precision injector (32) is connected with a piston pushing table (31) through a piston rod, and the upper part of the high-precision injector is communicated with a lower port (24-3) of an electromagnetic valve of the liquid separation module (2);
the liquid storage module (4) is positioned between the liquid distribution module (2) and the liquid transfer device module (3) and comprises a liquid storage column (41), the upper part of the liquid storage column (41) is connected with an electromagnetic valve internal interface (24-2) in the liquid distribution module (2), and the internal liquid is supplemented by the outside and is used for sucking a high-precision injector (32);
filling cell culture solution into a liquid storage column (41) for standby, and when the multichannel injection pump liquid distribution device works: the controller controls the stepping motor to enable the piston pushing table (31) to move downwards firstly, and controls the electromagnetic valve internal interface (24-2) connected with the liquid storage column (41) and the electromagnetic valve lower interface (24-3) connected with the high-precision injector (32) to be simultaneously opened, so that the liquid storage column (41) connected with the electromagnetic valve (23) is communicated with the high-precision injector (32), and at the moment, cell culture liquid enters the high-precision injector (32) from the liquid storage column (41); when injection and liquid separation are needed, the controller controls the stepping motor to enable the piston pushing table (31) to move upwards, controls the electromagnetic valve lower interface (24-3) connected with the high-precision injector (32) and the electromagnetic valve external interface (24-1) connected with the outside to be opened, enables the high-precision injector (32) connected with the electromagnetic valve (23) to be communicated with the outside, and enables cell culture liquid to enter corresponding cell culture parts from the high-precision injector (32) to be used for cell culture.
2. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the stepping motor (12) can accurately control the speed, the repeatability error is three thousandths, and a pulse type input is adopted to generate a fluctuation type flow scheme so as to meet the requirement of accurate cell culture and approach to the flow scheme in the human body.
3. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the high-precision injectors (32) are arranged in a square symmetrical mode, columns can be replaced, different injection flow requirements are met by columns of different sizes, and columns of different materials are suitable for nutrient solutions for organ culture.
4. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the liquid storage column (41) can be replaced by different materials so as to be suitable for various types of culture solutions, and a single liquid can be added for batch experiments, so that the efficiency is improved, or different types of culture solutions can be added for comparison experiments.
5. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the bottom disc (11) of the base module (1) is provided with supporting support plates (14) symmetrically distributed around the stepping motor (12) and used for pushing the piston to the table (31).
6. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the liquid storage module (4) further comprises a fixing bracket (42) and a liquid storage supporting plate (43), wherein the fixing bracket (42) is positioned between the liquid storage columns (41) and used for fixing the positions of the liquid storage columns (41) to keep stable; the liquid storage support plate (43) is positioned below the liquid storage column (41) and is used for supporting the liquid storage column (41).
7. A multi-channel syringe pump dispensing device for organoid culture according to claim 1, wherein: the cover plate (25) is also included, the size of the cover plate (25) is consistent with that of the hollow part of the top end bracket (22), and the hollow part can be tightly covered.
CN202310992594.8A 2023-08-08 2023-08-08 Multichannel injection pump liquid separating device for organoid culture Pending CN116855351A (en)

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Application Number Priority Date Filing Date Title
CN202310992594.8A CN116855351A (en) 2023-08-08 2023-08-08 Multichannel injection pump liquid separating device for organoid culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310992594.8A CN116855351A (en) 2023-08-08 2023-08-08 Multichannel injection pump liquid separating device for organoid culture

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CN116855351A true CN116855351A (en) 2023-10-10

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