CN220099060U - Constant temperature cell shaking table - Google Patents

Abstract

The utility model relates to the technical field of constant temperature cell shaking tables, and provides a constant temperature cell shaking table, which comprises: the device comprises a box body and a bearing component arranged in the box body, wherein a plurality of injection components are arranged on the upper side of the bearing component, and a supporting component is arranged in the box body on the upper side of the bearing component. A material conveying component is arranged above the supporting component and is used for providing raw materials required by the injection operation of the injection component. The top at the box is provided with push-and-pull subassembly, push-and-pull subassembly can be simultaneously to the push-and-pull rod on a plurality of injection subassembly push-and-pull for the piston can reciprocate in the solution section of thick bamboo, thereby can carry out the nutrient injection to a plurality of cell strain culture tubes simultaneously, and can stabilize accurate control and carry out the nutrient addition to every cell strain culture tube, make the quantity of adding the nutrient in every cell strain culture tube the same, ensure that every cell strain culture tube all can obtain the same and appropriate amount of nutrient, improve cell culture's survival rate greatly, have fine practicality.

Description

Constant temperature cell shaking table

Technical Field

The utility model relates to the technical field of constant temperature cell shaking tables, in particular to a constant temperature cell shaking table.

Background

The biological culture shaking table has the functions of stainless steel universal fixture, digital display temperature control, stepless speed regulation and good thermal circulation, is a multipurpose biochemical device, is a laboratory device which is indispensable for precise culture and preparation of scientific researches such as plants, organisms, microorganisms, genetics, viruses, environmental protection, medicine and the like, is suitable for the scientific research departments such as various universities, oil chemical industry, sanitation epidemic prevention, environmental monitoring and the like to perform oscillation culture on various liquid and solid compounds such as organisms, biochemistry, cells, strains and the like.

Through retrieval, the prior patent (bulletin number: CN 211771354U) discloses a constant temperature cell strain culture shaking table, which comprises a rack and a culture box, wherein the culture box comprises a box body, a first hinging seat, a second hinging seat, a culture tube erection plate, a cell strain culture tube and a constant temperature device; the incubator also comprises a nutrition liquid dripping device arranged in the incubator body, wherein the nutrition liquid dripping device comprises a clamping plate, a dropper and a hose, the two ends of the clamping plate are fixed with the incubator body, the dropper is correspondingly fixed on the clamping plate, and the hose is communicated with the dropper; the temperature in the incubator body is adjusted through the thermostat when the servo motor in the rack drives the incubator to swing, so that the cell strain can be normally cultured, in addition, the nutrient liquid dripping device is arranged in the incubator body, the nutrient liquid can be dripped while the cell strain is cultured, the manual dripping of the nutrient liquid in the incubator body is avoided, the influence on the culture of the cell strain is avoided, and the cell strain culture device has the advantages of realizing continuous culture of cells by meeting the cell strain culture environment and small damage to the cells during the cell culture.

In the scheme, the nutrient solution storage device is externally connected with the hose, the nutrient solution is fed into the dropper through the hose, and the dropper extends to the inside of the cell strain culture tube, so that the nutrient solution is directly converged into the cell strain culture tube. However, in this process, there is still a certain disadvantage:

1. the amount of the nutrient solution injected into the cell strain culture tube by each dropper cannot be well controlled, so that the nutrient solution received by each cell strain culture tube is different, the growth of cells can be influenced by excessive or insufficient nutrient solution, and the synchronization of each cell culture is poor due to different amounts of nutrient solution;

2. the dropper extends to the inside of the cell strain culture tube, so that the culture tube erection plate provided with the cell strain culture tube is inconvenient to withdraw from the inside of the box body in the later period, and the cell strain culture tube is inconvenient to process.

In view of this, the present utility model proposes a thermostatic cell shaker.

Disclosure of Invention

The utility model provides a constant temperature cell shaker, which solves the problem that the existing constant temperature cell shaker in the related technology can not stably and accurately add nutrients to a plurality of cell strain culture tubes at the same time.

The technical scheme of the utility model is as follows: a thermostatic cell shaker comprising: the cell culture device comprises a box body, a plurality of cell strain culture tubes arranged on the inner side of the box body and a constant temperature device arranged at the bottom of the box body, wherein a bearing assembly is arranged in the box body positioned on the upper side of the constant temperature device and used for placing the cell strain culture tubes;

the injection assemblies are arranged on the upper side of the bearing assembly, correspond to the cell strain culture tubes one by one, and are positioned right above the cell strain culture tubes and are used for injecting raw materials into the cell strain culture tubes;

a supporting component is arranged in the box body at the upper side of the bearing component and is used for installing the injection component;

a material conveying component is arranged above the supporting component and is used for providing raw materials required by the injection operation of the injection component;

the top of the box body is provided with a push-pull assembly for driving the injection assembly.

Preferably, the support assembly comprises two T-shaped rods, lifting plates are fixed on opposite inner walls of the T-shaped rods, T-shaped grooves are symmetrically formed in the box body, the T-shaped rods are in sliding fit with the T-shaped grooves, and supporting plates are sleeved between the lifting plates together.

Preferably, the support assembly further comprises a return spring, and two ends of the return spring are fixedly connected with the top of the T-shaped groove and the top of the T-shaped rod respectively.

Preferably, the material conveying component comprises a liquid conveying pipe, a liquid containing box and a liquid inlet pipe, wherein the liquid containing box is fixed at the top of the supporting plate, the liquid inlet pipe extends to the inside of the liquid containing box and is fixedly connected with the liquid containing box, a gap exists between the bottom of the liquid inlet pipe and the bottom of the liquid containing box, and the liquid conveying pipe extends to the inside of the box and is fixedly connected with the top of the liquid inlet pipe.

Preferably, the injection assembly comprises a through pipe, the through pipe extends to the inside of flourishing liquid box, layer board in proper order to with flourishing liquid box, layer board fixed connection, be located fixedly connected with solution section of thick bamboo on the layer board of siphunculus downside, solution section of thick bamboo is linked together with the siphunculus, just solution section of thick bamboo coincides with the axis of siphunculus, the bottom of solution section of thick bamboo is fixed with the injection burette that is linked together with the inside of solution section of thick bamboo, the junction of injection burette and solution section of thick bamboo is provided with first check valve, the injection burette extends to inside the cell strain culture tube.

Preferably, the injection assembly comprises a piston which is sleeved in the solution cylinder in a sliding manner, a push-pull rod is vertically fixed at the top of the piston, and the push-pull rod extends into the through pipe and is in sliding connection with the through pipe.

Preferably, a suction pipe is arranged between the solution cylinder and the solution containing box, extends into the supporting plate and is fixedly connected with the supporting plate, and a second one-way valve is arranged at the joint of the suction pipe and the solution cylinder.

Preferably, the push-pull assembly comprises a lifting table arranged on the inner side of the box body, the lifting table is fixedly connected with the push-pull rod, guide rods are vertically fixed at four corners of the top of the lifting table, and the guide rods extend to the top of the box body and are in sliding connection with the box body.

Preferably, a transmission part is arranged between the lifting table and the box body, the transmission part comprises a fixed frame fixed at the center of the top of the lifting table, a rotating block is sleeved in the fixed frame in a rotating mode, a screw is fixed at the top of the rotating block, the screw is rotationally connected to the top of the fixed frame, the screw is sleeved at the top of the box body in a threaded mode, and a turntable is fixed at the top of the screw.

Preferably, the supporting component comprises supporting bars symmetrically fixed in the box body, the upper ends of the two supporting bars are jointly overlapped with a bracket for placing the cell strain culture tube, a supporting table for supporting the bottom of the cell strain culture tube is fixed at the bottom of the bracket, and the bottom of the lifting plate is abutted to the bottom of the bracket.

The working principle and the beneficial effects of the utility model are as follows:

1. in the utility model, a plurality of injection assemblies are arranged on the upper side of the bearing assembly, the injection assemblies are in one-to-one correspondence with the cell strain culture tubes and are positioned right above the cell strain culture tubes, and a supporting assembly is arranged in the box body on the upper side of the bearing assembly and used for installing the injection assemblies. A material conveying component is arranged above the supporting component and is used for providing raw materials required by the injection operation of the injection component. The top of the box body is provided with the push-pull assembly, push-pull rods on the injection assemblies can be pushed and pulled simultaneously through the push-pull assembly, so that the piston can move up and down in the solution cylinder, nutrient injection can be carried out on the cell strain culture tubes simultaneously, and nutrient addition can be carried out on each cell strain culture tube under stable and accurate control, so that the nutrient addition amount in each cell strain culture tube is the same, the same and proper nutrient can be obtained for each cell strain culture tube, the survival rate of cell culture is greatly improved, and the cell strain culture device has good practical performance;

2. according to the utility model, after the culture work is finished, the supporting plate on the supporting component is lifted, and the supporting plate is lifted, so that the injection dropper on the injection component can be moved out of the top of the cell strain culture tube, the blocking of the injection dropper on the movement of the cell strain culture tube is effectively avoided, and the bracket with the cell strain culture tube can be smoothly pulled out from the inside of the box body.

Drawings

The utility model will be described in further detail with reference to the drawings and the detailed description.

FIG. 1 is a schematic diagram showing the structure of a constant temperature cell shaker according to the present utility model;

FIG. 2 is an enlarged schematic view of the structure of FIG. 1A;

FIG. 3 is a schematic view of a partial cross-sectional structure of a pallet and a liquid container according to the present utility model;

FIG. 4 is a schematic diagram showing the structural components of the transmission member according to the present utility model;

in the figure: 1. a cell line culture tube; 2. an injection assembly; 21. a piston; 22. injection droppers; 23. a first one-way valve; 24. a suction pipe; 25. a solution cylinder; 26. a second one-way valve; 27. a through pipe; 28. a push-pull rod; 3. a support assembly; 31. a T-shaped groove; 32. a return spring; 33. a T-shaped rod; 34. a lifting plate; 35. a supporting plate; 4. a material conveying component; 41. an infusion tube; 42. a liquid containing box; 43. a liquid inlet pipe; 5. a push-pull assembly; 51. a guide rod; 52. a lifting table; 53. a transmission member; 531. a turntable; 532. a screw; 533. a fixed frame; 534. a rotating block; 6. a case; 7. a support assembly; 71. a bracket; 72. a support; 73. a support bar; 8. and a constant temperature device.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below in conjunction with the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Referring to fig. 1, a constant temperature cell shaker, comprising: the cell strain culturing device comprises a box body 6, a plurality of cell strain culturing tubes 1 arranged on the inner side of the box body 6 and a constant temperature device 8 arranged at the bottom of the box body 6, wherein a bearing assembly 7 is arranged in the box body 6 positioned on the upper side of the constant temperature device 8 and used for placing the cell strain culturing tubes 1. Wherein, the supporting component 7 comprises supporting bars 73 symmetrically fixed in the box 6, the upper ends of the two supporting bars 73 are jointly lapped with a bracket 71 for placing the cell strain culture tube 1, and the bottom of the bracket 71 is fixed with a supporting table 72 for supporting the bottom of the cell strain culture tube 1.

Referring to fig. 1, 2 and 3, a plurality of injection assemblies 2 are disposed above the support assembly 7, the injection assemblies 2 are in one-to-one correspondence with the cell strain culture tubes 1, and the injection assemblies 2 are disposed right above the cell strain culture tubes 1 for injecting raw materials into the cell strain culture tubes 1. Inside the housing 6 on the upper side of the holding assembly 7 is provided a support assembly 3 for mounting the injection assembly 2.

Wherein, the supporting component 3 includes two T-shaped rods 33, and two T-shaped rods 33 are all fixed with lifter plate 34 to the inner wall, lifter plate 34 bottom and bracket 71 bottom looks butt for bracket 71 can be steady slide in box 6 inside under the combined action of support bar 73 and lifter plate 34. T-shaped grooves 31 are symmetrically formed in the box body 6, T-shaped rods 33 are in sliding fit with the T-shaped grooves 31, and supporting plates 35 are sleeved between the two lifting plates 34. The supporting component 3 further comprises a return spring 32, and two ends of the return spring 32 are fixedly connected with the top of the T-shaped groove 31 and the top of the T-shaped rod 33 respectively. By lifting the supporting plate 35, the supporting plate 35 drives the two lifting plates 34 to move upwards, the lifting plates 34 can stably move up and down under the combined action of the T-shaped grooves 31 and the T-shaped rods 33, and the lifting plates 35 can lift the injection assembly 2 upwards, so that the bracket 71 provided with the cell strain culture tubes 1 can be conveniently pulled out from the inside of the box 6.

Above the support assembly 3 is arranged a feed assembly 4 for providing the raw material required for the injection operation of the injection assembly 2. The material conveying assembly 4 comprises a liquid conveying pipe 41, a liquid containing box 42 and a liquid inlet pipe 43, wherein the liquid containing box 42 is fixed at the top of the supporting plate 35, the liquid inlet pipe 43 extends into the liquid containing box 42 and is fixedly connected with the liquid containing box 42, a gap exists between the bottom of the liquid inlet pipe 43 and the bottom of the liquid containing box 42, and the liquid conveying pipe 41 extends into the box 6 and is fixedly connected with the top of the liquid inlet pipe 43. Nutrients required by cells are fed into the liquid inlet pipe 43 through the liquid inlet pipe 41 and discharged into the liquid containing box 42 through a gap between the bottom of the liquid inlet pipe 43 and the bottom of the liquid containing box 42.

The injection assembly 2 comprises a through pipe 27, the through pipe 27 sequentially extends into the liquid containing box 42 and the supporting plate 35, the liquid containing box 42 and the supporting plate 35 are fixedly connected, a solution cylinder 25 is fixedly connected to the supporting plate 35 at the lower side of the through pipe 27, the solution cylinder 25 is communicated with the through pipe 27, the solution cylinder 25 coincides with the central axis of the through pipe 27, an injection dropper 22 communicated with the inside of the solution cylinder 25 is fixed at the bottom of the solution cylinder 25, a first one-way valve 23 is arranged at the joint of the injection dropper 22 and the solution cylinder 25, so that solution in the solution cylinder 25 can only be discharged through the injection dropper 22, the solution in the injection dropper 22 cannot enter the solution cylinder 25, and the injection dropper 22 extends into the cell strain culture tube 1. The injection assembly 2 further comprises a piston 21 slidably sleeved in the solution cylinder 25, a push-pull rod 28 is vertically fixed to the top of the piston 21, and the push-pull rod 28 extends into the through pipe 27 and is slidably connected with the through pipe 27. A suction pipe 24 is arranged between the solution cylinder 25 and the solution containing box 42, the suction pipe 24 extends into the support plate 35 and is fixedly connected with the support plate 35, and a second one-way valve 26 is arranged at the joint of the suction pipe 24 and the solution cylinder 25, so that the solution in the solution containing box 42 can only be discharged through the suction pipe 24, and the solution in the suction pipe 24 cannot enter the solution containing box 42. When the piston 21 moves up, the first check valve 23 is closed, the second check valve 26 is opened, the solution in the solution container 42 can be injected into the solution cylinder 25 through the suction pipe 24, and when the piston 21 moves down, the first check valve 23 is opened, the second check valve 26 is closed, and the piston 21 extrudes the solution stored in the solution cylinder 25 through the injection dropper 22.

Referring to fig. 1, 2 and 4, a push-pull assembly 5 is provided on top of the housing 6 for driving the injection assembly 2. The push-pull assembly 5 comprises a lifting table 52 arranged on the inner side of the box body 6, the lifting table 52 is fixedly connected with the push-pull rod 28, guide rods 51 are vertically fixed at four corners of the top of the lifting table 52, and the guide rods 51 extend to the top of the box body 6 and are in sliding connection with the box body 6. A transmission part 53 is arranged between the lifting table 52 and the box body 6, the transmission part 53 comprises a fixed frame 533 fixed at the center of the top of the lifting table 52, a rotating block 534 is rotatably sleeved in the fixed frame 533, a screw 532 is fixed at the top of the rotating block 534, the screw 532 is rotatably connected to the top of the fixed frame 533, the screw 532 is in threaded sleeve arrangement at the top of the box body 6, and a turntable 531 is fixed at the top of the screw 532. Screw 532 is rotated through carousel 531, and screw 532 drives rotatory piece 534 and rotates in fixed frame 533, and screw 532 reciprocates along box 6 top, carries out the push-and-pull to fixed frame 533 through rotatory piece 534 to can go up and down to elevating platform 52, carries out the push-and-pull to push-and-pull rod 28 through elevating platform 52.

Working principle and using flow: in operation, nutrients required by cells are fed into the liquid inlet pipe 43 through the liquid conveying pipe 41, and are discharged into the liquid containing box 42 for storage through a gap between the bottom of the liquid inlet pipe 43 and the bottom of the liquid containing box 42. When nutrients are added, the screw 532 is rotated by the turntable 531, the screw 532 drives the rotating block 534 to rotate inside the fixed frame 533, the screw 532 moves up and down along the top of the box 6, the fixed frame 533 is pushed and pulled by the rotating block 534, so that the lifting table 52 can be lifted, the push-pull rod 28 is moved up by the lifting table 52, the piston 21 moves up in the solution cylinder 25, at the moment, the first one-way valve 23 is closed, the second one-way valve 26 is opened, the solution in the liquid containing box 42 can be injected into the solution cylinder 25 through the suction pipe 24, the push-pull rod 28 is moved down by the lifting table 52, the piston 21 moves down in the solution cylinder 25, at the moment, the first one-way valve 23 is opened, the second one-way valve 26 is closed, the solution stored in the solution cylinder 25 is extruded by the injection dropper 22 by the piston 21, nutrients can be injected into a plurality of cell strain culture pipes 1 simultaneously, and the nutrients can be added into each cell strain culture pipe 1 under stable and accurate control, so that the added nutrients in each cell strain culture pipe 1 are the same. After the cultivation is finished, the supporting plate 35 is lifted, the supporting plate 35 drives the two lifting plates 34 to move upwards, under the combined action of the T-shaped groove 31 and the T-shaped rod 33, the lifting plates 34 can stably move up and down, the supporting plate 35 can lift the injection assembly 2 upwards, the injection dropper 22 moves out of the top of the cell strain culture tube 1, and at the moment, the bracket 71 provided with the cell strain culture tube 1 can be smoothly pulled out from the inside of the box 6.

The foregoing description of the preferred embodiments of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.

Claims (9)

1. A thermostatic cell shaker comprising: the cell strain culture device comprises a box body (6), a plurality of cell strain culture tubes (1) arranged on the inner side of the box body (6) and a constant temperature device (8) arranged at the bottom of the box body (6), and is characterized in that a bearing component (7) is arranged in the box body (6) on the upper side of the constant temperature device (8) and used for placing the plurality of cell strain culture tubes (1);

a plurality of injection assemblies (2) are arranged on the upper side of the bearing assembly (7), the injection assemblies (2) are in one-to-one correspondence with the cell strain culture tubes (1), and the injection assemblies (2) are positioned right above the cell strain culture tubes (1) and are used for injecting raw materials into the cell strain culture tubes (1);

a supporting component (3) is arranged in the box body (6) at the upper side of the bearing component (7) and is used for installing the injection component (2);

a material conveying component (4) is arranged above the supporting component (3) and is used for providing raw materials required by the injection operation of the injection component (2);

the top of the box body (6) is provided with a push-pull assembly (5) for driving the injection assembly (2);

the supporting component (3) comprises two T-shaped rods (33), lifting plates (34) are fixed on opposite inner walls of the T-shaped rods (33), T-shaped grooves (31) are symmetrically formed in the box body (6), the T-shaped rods (33) are in sliding fit with the T-shaped grooves (31), and supporting plates (35) are sleeved between the lifting plates (34) together.

2. The constant temperature cell shaker as claimed in claim 1, wherein the support assembly (3) further comprises a return spring (32), and two ends of the return spring (32) are fixedly connected with the top of the T-shaped groove (31) and the top of the T-shaped rod (33), respectively.

3. The constant temperature cell shaker as claimed in claim 1, wherein the material conveying component (4) comprises a liquid conveying pipe (41), a liquid containing box (42) and a liquid inlet pipe (43), the liquid containing box (42) is fixed at the top of the supporting plate (35), the liquid inlet pipe (43) extends to the inside of the liquid containing box (42) and is fixedly connected with the liquid containing box, a gap exists between the bottom of the liquid inlet pipe (43) and the bottom of the liquid containing box (42), and the liquid conveying pipe (41) extends to the inside of the box (6) and is fixedly connected with the top of the liquid inlet pipe (43).

4. The constant temperature cell shaker of claim 1, wherein the injection assembly (2) comprises a through pipe (27), the through pipe (27) sequentially extends to the inside of the liquid containing box (42) and the supporting plate (35), the injection assembly is fixedly connected with the liquid containing box (42) and the supporting plate (35), the supporting plate (35) positioned at the lower side of the through pipe (27) is fixedly connected with a solution cylinder (25), the solution cylinder (25) is communicated with the through pipe (27), the solution cylinder (25) coincides with the central axis of the through pipe (27), an injection dropper (22) communicated with the inside of the solution cylinder (25) is fixed at the bottom of the solution cylinder (25), a first one-way valve (23) is arranged at the joint of the injection dropper (22) and the solution cylinder (25), and the injection dropper (22) extends to the inside of the cell strain culture tube (1).

5. A thermostatic cell shaker as claimed in claim 1, wherein the injection assembly (2) comprises a piston (21) slidably fitted inside a solution cylinder (25), a push-pull rod (28) being vertically fixed to the top of the piston (21), the push-pull rod (28) extending into the tube (27) and being slidably connected to the tube (27).

6. The thermostatic cell shaker as claimed in claim 5, characterized in that a suction tube (24) is arranged between the solution cylinder (25) and the liquid-containing box (42), the suction tube (24) extends into the support plate (35) and is fixedly connected with the support plate (35), and a second one-way valve (26) is arranged at the joint of the suction tube (24) and the solution cylinder (25).

7. The constant temperature cell shaker of claim 1, wherein the push-pull assembly (5) comprises a lifting table (52) arranged on the inner side of the box body (6), the lifting table (52) is fixedly connected with a push-pull rod (28), guide rods (51) are vertically fixed at four corners of the top of the lifting table (52), and the guide rods (51) extend to the top of the box body (6) and are in sliding connection with the box body (6).

8. The constant temperature cell shaker of claim 7, wherein a transmission part (53) is arranged between the lifting table (52) and the box body (6), the transmission part (53) comprises a fixed frame (533) fixed at the center of the top of the lifting table (52), a rotating block (534) is rotatably sleeved in the fixed frame (533), a screw (532) is fixed at the top of the rotating block (534), the screw (532) is rotatably connected to the top of the fixed frame (533), the screw (532) is sleeved at the top of the box body (6) in a threaded manner, and a turntable (531) is fixed at the top of the screw (532).

9. A thermostatic cell shaker as claimed in claim 1, wherein the support assembly (7) comprises support bars (73) symmetrically fixed inside the casing (6), brackets (71) for placing the cell strain culture tubes (1) are commonly lapped at the upper ends of the two support bars (73), a supporting table (72) for supporting the bottoms of the cell strain culture tubes (1) is fixed at the bottoms of the brackets (71), and the bottoms of the lifting plates (34) are abutted against the bottoms of the brackets (71).