CN220300746U

CN220300746U - Stem cell culture dish convenient to change culture solution

Info

Publication number: CN220300746U
Application number: CN202321796435.2U
Authority: CN
Inventors: 王宝金; 夏雨
Original assignee: Kunming Quangang Biotechnology Co ltd
Current assignee: Kunming Quangang Biotechnology Co ltd
Priority date: 2023-07-10
Filing date: 2023-07-10
Publication date: 2024-01-05
Anticipated expiration: 2033-07-10

Abstract

The utility model discloses a stem cell culture dish convenient for replacing culture solution, which relates to the technical field of culture dishes and comprises a base, wherein the culture dish is movably connected inside the base, a filter screen is fixedly connected inside the bottom end of the culture dish, a group of bilaterally symmetrical connecting grooves are movably connected on the inner wall of the base, and a limiting structure is movably connected inside the connecting grooves. According to the utility model, the limiting structure is arranged, so that the bottom end of the limiting structure acts on the limiting block, the top end of the limiting strip moves to the opposite side until the limiting block is vertical, meanwhile, the side length of the spring is stretched, when the culture dish moves to the bottom end of the base, the reaction of the spring and the limiting strip reset the limiting strip, so that the top end of the limiting strip clamps the culture dish, when the culture dish needs to be taken out, the tool is used for supporting the limiting strip inwards to be vertical, and then the culture dish is taken out, so that convenience in changing culture solution is realized, and the connection stability of the culture dish and the base is enhanced.

Description

Stem cell culture dish convenient to change culture solution

Technical Field

The utility model relates to the technical field of culture dishes, in particular to a stem cell culture dish convenient for replacing culture solution.

Background

A dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disk-shaped bottom and a lid, generally made of glass or plastic. The materials of the culture dishes are basically divided into two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be polyethylene material, has disposable and multiple use, is suitable for laboratory inoculation, streaking and bacteria separation, and may be used in plant material cultivation.

In the practical operation process, the person in the field finds that in the stem cell culture operation process, the nutrient solution in the culture dish needs to be replaced at regular time, but the existing mode is to pour the nutrient solution in the culture dish to replace after taking out the stem cells in the culture dish, so that the conditions of stem cell death and the like caused by the increase of operation difficulty and excessive force in the operation process occur, and the growth of the stem cells can be influenced by the replacement of the nutrient solution which is used after the culture dish is remained. To this end we provide a stem cell culture dish which facilitates the replacement of the culture solution, solving the above problems.

Disclosure of Invention

The utility model aims to make up the defects of the prior art and provides a stem cell culture dish convenient for replacing culture solution.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a stem cell culture dish convenient to change culture solution, includes the base, the inside swing joint of base has the culture dish, the inside fixedly connected with filter screen of bottom of culture dish, the inside swing joint of the inner wall swing joint of base a set of bilateral symmetry spread groove, and the inside swing joint of spread groove has limit structure, the bottom fixedly connected with cleaning device of base, the fixedly connected with fluid-discharge tube of base, and the top of fluid-discharge tube extends to the inside of base, the outside fixedly connected with several supporting leg of base.

Above-mentioned, limit structure includes connecting axle, spring and spacing, connecting axle fixed connection is in the bottom surface intermediate position of spacing, spring fixed connection is in the surface of spacing, and the spring is located the homonymy with the connecting axle.

Above-mentioned, connecting axle and spring all are located the inside of spread groove, the both ends of connecting axle are all swing joint in the inner wall of spread groove, and the one end fixed connection in the inside of spread groove that the stopper was kept away from to the spring, two the spacing is the setting of falling the eight characters.

Above-mentioned, cleaning device includes servo motor, connecting block and clearance strip, connecting block fixed connection is in servo motor's output, and clearance strip fixedly connected with connecting block's the outside.

Above-mentioned, servo motor fixed connection is in the bottom of base, connecting block and clearance strip all are located the inside bottom of base, and servo motor output runs through base and connecting block fixed connection.

Above-mentioned, the bottom of base is the funnel form, the clearance strip is the L font, the outside of clearance strip is hugged closely the base bottom inner wall.

Compared with the prior art, the stem cell culture dish convenient for replacing the culture solution has the following beneficial effects:

1. according to the utility model, the culture dish is pressed downwards through the limiting structure, so that the bottom end of the culture dish acts on the limiting block, the top end of the limiting strip moves to the opposite side until the limiting strip is vertical, meanwhile, the side length of the spring is stretched, when the culture dish moves to the bottom end of the base, the reaction of the spring and the limiting strip reset the limiting strip, so that the top end of the limiting strip clamps the culture dish, when the culture dish needs to be taken out, the tool is used for supporting the limiting strip inwards to be vertical, and then the culture dish is taken out, so that convenience in changing culture solution is brought to take out the culture dish and the connection stability of the culture dish and the base is enhanced.

2. According to the utility model, the cleaning device is arranged, the servo motor is used for driving the connecting block to rotate, so that the cleaning strip is driven to hang and take the residual nutrient solution in the bottom end of the base, and the residual nutrient solution is discharged through the liquid discharge pipe, thereby being beneficial to preventing the residual used nutrient solution in the base when the nutrient solution is replaced, and further causing the quality degradation of the newly replaced nutrient solution to lead to the unsatisfactory stem cell culture.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model.

Drawings

FIG. 1 is a schematic perspective view of the present utility model;

FIG. 2 is a schematic cross-sectional view of the present utility model;

FIG. 3 is an enlarged schematic view of the structure of FIG. 2A according to the present utility model;

fig. 4 is an enlarged schematic view of the structure of fig. 2B according to the present utility model.

In the figure: 1. a base; 2. a culture dish; 3. a filter screen; 4. a connecting groove; 5. a limit structure; 501. a connecting shaft; 502. a spring; 503. a limit bar; 6. a cleaning device; 601. a servo motor; 602. a connecting block; 603. cleaning the strip; 7. a liquid discharge pipe; 8. and (5) supporting legs.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

As shown in fig. 1-4, the present utility model provides a technical solution: the utility model provides a stem cell culture dish convenient to change culture solution, including base 1, the inside swing joint of base 1 has culture dish 2, the inside fixedly connected with filter screen 3 of bottom of culture dish 2, the inside swing joint of base 1 a set of bilateral symmetry spread groove 4, and the inside swing joint of spread groove 4 has limit structure 5, the bottom fixedly connected with cleaning device 6 of base 1, the fixedly connected with fluid-discharge tube 7 of base 1, and the top of fluid-discharge tube 7 extends to the inside of base 1, the outside fixedly connected with several supporting leg 8 of base 1.

The culture dish 2 is pressed downwards to make on its bottom effect and the stopper, limit 503 top is to opposite side motion until vertical this moment, simultaneously the tensile side length of spring 502, when culture dish 2 moves to base 1 bottom, spring 502 reaction makes it reset with limit 503 this moment, thereby make the top of limit 503 block culture dish 2, when need take out culture dish 2, utilize the instrument inwards to withhold limit 503's fact vertical, take out culture dish 2 afterwards, be favorable to taking out culture dish 2 and reinforcing culture dish 2 and base 1 connection stability when conveniently changing the culture solution again drive connecting block 602 through servo motor 601 and rotate, thereby drive clearance strip 603 and hang the inside remaining nutrient solution in base 1 bottom, thereby make it discharge through fluid-discharge tube 7, prevent remaining used nutrient solution in the base 1 when being favorable to changing the nutrient solution, thereby cause the new nutrient solution quality decline that changes and lead to the culture stem cell to be less than ideal.

As shown in fig. 1-3, the limiting structure 5 includes a connecting shaft 501, a spring 502 and a limiting bar 503, wherein the connecting shaft 501 is fixedly connected to the middle position of the bottom end surface of the limiting bar 503, the spring 502 is fixedly connected to the surface of the limiting bar 503, the spring 502 and the connecting shaft 501 are located on the same side, the connecting shaft 501 and the spring 502 are located inside the connecting groove 4, two ends of the connecting shaft 501 are movably connected to the inner wall of the connecting groove 4, one end of the spring 502, away from the limiting block, is fixedly connected to the inside of the connecting groove 4, and the two limiting bars 503 are arranged in an inverted splayed shape.

The culture dish 2 is pressed downwards to make on its bottom effect and the stopper, limit 503 top is to opposite side motion until vertical this moment, and the tensile side length of spring 502 simultaneously, when culture dish 2 moves to base 1 bottom, spring 502 reaction makes it reset with limit 503 this moment, thereby make the top of limit 503 block culture dish 2, when need take out culture dish 2, utilize the instrument inwards to withhold limit 503's fact vertical, take out culture dish 2 afterwards, take out culture dish 2 and reinforcing culture dish 2 and base 1 connection stability when being favorable to conveniently changing the culture solution.

As shown in fig. 1, fig. 2 and fig. 4, the cleaning device 6 includes a servo motor 601, a connecting block 602 and a cleaning bar 603, the connecting block 602 is fixedly connected to the output end of the servo motor 601, the cleaning bar 603 is fixedly connected to the outer side of the connecting block 602, the servo motor 601 is fixedly connected to the bottom end of the base 1, the connecting block 602 and the cleaning bar 603 are located at the inner bottom end of the base 1, the output end of the servo motor 601 penetrates through the base 1 and is fixedly connected with the connecting block 602, the bottom end of the base 1 is funnel-shaped, the cleaning bar 603 is L-shaped, and the outer side of the cleaning bar 603 is tightly attached to the inner wall of the bottom end of the base 1.

The connecting block 602 is driven to rotate through the servo motor 601, so that the cleaning strip 603 is driven to hang and take residual nutrient solution in the bottom end of the base 1, the residual nutrient solution is discharged through the liquid discharge pipe 7, the used nutrient solution is prevented from remaining in the base 1 when the nutrient solution is replaced, and the quality of the newly replaced nutrient solution is reduced, so that the cultured stem cells are not ideal enough.

Working principle: the culture dish 2 is pressed downwards to make on its bottom effect and the stopper, limit 503 top is to opposite side motion until vertical this moment, the tensile side length of spring 502 simultaneously, when culture dish 2 moves to base 1 bottom, spring 502 reaction makes it reset with limit 503 this moment, thereby make the top of limit 503 block culture dish 2, when need take out culture dish 2, utilize the instrument inwards to withhold limit 503's fact vertical, take out culture dish 2 afterwards, rethread servo motor 601 drives connecting block 602 and rotates, thereby drive clearance strip 603 and hang the inside remaining nutrient solution in base 1 bottom, make it thereby discharge through fluid-discharge tube 7.

It should be noted that, in this document, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless explicitly specified and limited otherwise, the terms "fixedly attached," "mounted," "connected," and "coupled" are to be construed broadly, e.g., as a fixed connection, as a removable connection, or as an integral connection; "coupled" may be either mechanical or electrical; the "connection" may be direct, indirect via an intermediary, or communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a stem cell culture dish convenient to change culture solution, includes base (1), its characterized in that: inside swing joint of base (1) has culture dish (2), the inside fixedly connected with filter screen (3) of the bottom of culture dish (2), the inside swing joint of the inner wall swing joint of base (1) a set of bilateral symmetry spread groove (4), and the inside swing joint of spread groove (4) has limit structure (5), the bottom fixedly connected with cleaning device (6) of base (1), the fixedly connected with fluid-discharge tube (7) of base (1), and the inside that the top of fluid-discharge tube (7) extends to base (1), the outside fixedly connected with several supporting leg (8) of base (1).

2. A stem cell culture dish for facilitating replacement of culture fluid according to claim 1, wherein: the limiting structure (5) comprises a connecting shaft (501), a spring (502) and a limiting strip (503), wherein the connecting shaft (501) is fixedly connected to the middle position of the bottom end surface of the limiting strip (503), the spring (502) is fixedly connected to the surface of the limiting strip (503), and the spring (502) and the connecting shaft (501) are located on the same side.

3. A stem cell culture dish for facilitating replacement of culture fluid according to claim 2, wherein: the connecting shaft (501) and the spring (502) are both positioned in the connecting groove (4), two ends of the connecting shaft (501) are both movably connected to the inner wall of the connecting groove (4), one end, away from the limiting block, of the spring (502) is fixedly connected to the inner part of the connecting groove (4), and two limiting strips (503) are arranged in an inverted splayed mode.

4. A stem cell culture dish for facilitating replacement of culture fluid according to claim 1, wherein: the cleaning device (6) comprises a servo motor (601), a connecting block (602) and a cleaning strip (603), wherein the connecting block (602) is fixedly connected to the output end of the servo motor (601), and the cleaning strip (603) is fixedly connected with the outer side of the connecting block (602).

5. The stem cell culture dish for facilitating replacement of culture medium according to claim 4, wherein: the servo motor (601) is fixedly connected to the bottom end of the base (1), the connecting block (602) and the cleaning strip (603) are both located at the inner bottom end of the base (1), and the output end of the servo motor (601) penetrates through the base (1) and is fixedly connected with the connecting block (602).

6. The stem cell culture dish for facilitating replacement of culture medium according to claim 4, wherein: the bottom of base (1) is the funnel form, clearance strip (603) are the L font, the outside of clearance strip (603) is hugged closely base (1) bottom inner wall.