CN210176875U - Plant cell culture fermentation cylinder - Google Patents
Plant cell culture fermentation cylinder Download PDFInfo
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- CN210176875U CN210176875U CN201920629715.1U CN201920629715U CN210176875U CN 210176875 U CN210176875 U CN 210176875U CN 201920629715 U CN201920629715 U CN 201920629715U CN 210176875 U CN210176875 U CN 210176875U
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- stirring blade
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Abstract
The utility model provides a plant cell culture fermentation tank, which achieves the effects of increasing dissolved oxygen and improving fluidity through a stirring device; the shearing force is reduced by utilizing the first stirring component and the second stirring component, so that the plant cells are prevented from autolysis; the stirring area is increased by using the auxiliary fins A and the like, so that the sufficient oxygen dissolution can be ensured at a low rotating speed; a driving structure is formed by the first motor, the second motor, the first gear, the second gear and the like; the first motor is used for driving the second gear to rotate, the second gear is meshed with the first gear, and the stirring shaft rotates, so that the first stirring assembly and the second stirring assembly are stirred; utilize the rotation of second motor, drive the transmission shaft at Z direction seat reciprocating motion, first gear and second gear are in the engaged state always in the time of the motion, make agitating unit rise or descend the in-process, can overturn the plant cell culture solution in the fermentation cylinder, improve the mobility of fermentation cylinder to increase dissolved oxygen volume, even oxygen.
Description
Technical Field
The utility model relates to a fermentation cylinder field, concretely relates to plant cell culture fermentation cylinder.
Background
Plant cell culture techniques include various plant cell culture techniques developed by culturing plant organs (roots, branches and leaves, hairy roots, embryos, etc.), tissues, cells and protoplasts.
Plant cell culture has the following advantages: firstly, the production of metabolites is completely carried out under the condition of artificial control, and the metabolites with the super-whole plant yield are obtained by changing the culture conditions and selecting an excellent culture system; secondly, the culture is carried out under the aseptic condition, so that the invasion of germs and insect pests can be eliminated; thirdly, specific biotransformation reactions can be carried out; fourthly, new synthetic routes can be explored and new useful substances can be obtained.
Plant cell culture is generally carried out in a fermentation tank, and the plant cells belong to autotrophic type, and the fermentation tank is required to have good oxygen transfer, good fluidity and lower shearing force. In order to ensure oxygen transfer and fluidity in the fermentation tank, plant cell culture is generally mixed by stirring rotation speed, but the shear force of the existing stirring device can seriously damage cell membranes and cause cell dissolution under the condition of high rotation speed, thereby causing the failure of plant cell culture.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model provides a plant cell culture fermentation tank which has the characteristics of small shearing force and high cell culture success rate.
The utility model provides a technical scheme of above-mentioned problem does: a plant cell culture fermentation tank comprises a tank body, a base, a tank cover, a stirring device and a supporting table;
the tank cover and the sealing plate are made of stainless steel, the tank body is made of glass, and the tank cover is hermetically connected with the upper end face of the tank body; the sealing plate is connected with the lower end face of the tank body;
the tank cover is provided with a feed inlet, and the tank inlet cover is provided with a plurality of connectors; a discharge hole is formed in the lower part of the tank body; the bottom of the tank body is provided with an air distributor, the air distributor is connected with the air tank through a pipeline, and the pipeline is provided with a valve;
the stirring device is positioned in the tank body and comprises a stirring shaft, a first stirring assembly and a second stirring assembly;
the first stirring components are uniformly distributed along the Z direction and comprise a central plate, first stirring blades and stirring fins
The central plate is fixed on the stirring shaft, and the central axis of the central plate is superposed with the central axis of the stirring shaft; the central plate comprises an arc surface A and an arc surface B, the arc surface A and the arc surface B are vertically symmetrical, the arc surface A is positioned above the arc surface B, the arc surface A and the arc surface B are connected into a whole, and the joint is in arc transition;
the first stirring blades are annularly arrayed around the central axis of the central plate, each first stirring blade comprises an upper stirring blade and a lower stirring blade, and the upper stirring blades and the lower stirring blades are vertically symmetrical about the central plate; the upper stirring blade comprises a stirring blade A and a stirring blade B, one end of the stirring blade A is connected with the arc surface A, the other end of the stirring blade A is connected with the stirring blade B into a whole, the stirring blade A and the stirring blade B are vertically symmetrical, the stirring blade A is of an upwards-convex arc structure, and the section of the stirring blade A in the vertical plane direction is circular;
a plurality of stirring fins are arranged between the upper stirring blade and the lower stirring blade, and two ends of each stirring fin are respectively connected with the upper stirring blade and the lower stirring blade; the stirring fins are of an outward convex arc structure, and the cross sections of the stirring fins in the horizontal plane direction are circular;
an auxiliary fin A is arranged above the upper stirring blade, the auxiliary fin A is of an arc structure, and two ends of the auxiliary fin A are respectively connected with the stirring blade B;
an auxiliary fin B is arranged below the lower stirring blade, the auxiliary fin B is of a circular arc structure, and two ends of the auxiliary fin B are respectively connected with the stirring blade B; the stirring blade B and the stirring blade A are vertically symmetrical;
the lower end of the stirring shaft is connected with a second stirring component, and the second stirring component is positioned below the first stirring component.
Furthermore, the second stirring assembly comprises an intermediate piece and stirring blades, the intermediate piece is fixed on the stirring shaft and comprises an annular plate and a bottom plate, the annular plate is of a concave annular structure, and the inner diameter of the annular plate is gradually increased from top to bottom along the Z direction; the lower end surface of the annular plate is connected with the bottom plate, and the connection part is in arc transition; the annular plate is provided with a plurality of stirring pieces, the stirring pieces surround the annular array of the central axis of the annular plate, the stirring pieces are of an outward convex arc structure, and the cross sections of the stirring pieces in the horizontal plane direction are circular.
Further, the upper end of the stirring shaft penetrates through the tank cover to be connected with the transmission shaft, the stirring shaft is rotationally connected with the tank cover, and the connection part is mechanically sealed; one end of the transmission shaft, which is far away from the stirring shaft, is rotatably connected with a bearing seat, the bearing seat is fixed on a support frame, and the support frame is fixed on the tank cover;
the transmission shaft is connected with a first gear, the first gear is meshed with a second gear, the second gear is fixed on a first motor shaft, and the first motor is fixed on the support frame;
even there is antifriction bearing on the transmission shaft, and antifriction bearing is located first gear below, even has the U-shaped frame on the antifriction bearing, and the one end that antifriction bearing was kept away from to the U-shaped frame even has the actuating lever, and the actuating lever is connected with second motor shaft, and the second motor is fixed on the support frame.
Further, the first motor and the second motor are both stepping motors.
The utility model discloses beneficial effect has:
(1) the utility model achieves the functions of increasing dissolved oxygen and improving fluidity through the stirring device; the first stirring component and the second stirring component in the shape of circular arcs are used for reducing shearing force, so that plant cells are prevented from autolysis; the auxiliary fins A, the auxiliary fins B and the stirring sheets are used for increasing the stirring area, so that the sufficient oxygen dissolution can be ensured at a low rotating speed, and the sufficient fermentation of the plant cell culture solution is ensured.
(2) The utility model forms a driving structure of the stirring device through the first motor, the second motor, the first gear, the second gear and the like; the first motor is used for driving the second gear to rotate, the second gear is meshed with the first gear, and the stirring shaft rotates, so that the first stirring component and the second stirring component stir the plant cell sap in the fermentation tank; the height of the transmission shaft is driven to change by the rotation of the second motor, so that the transmission shaft reciprocates in the Z-direction seat, the first gear and the second gear are always in a meshed state during movement, the stirring device can turn over the plant cell culture solution in the fermentation tank in the ascending or descending process, the structure of the intermediate part is utilized to ensure that the plant cell wall is not damaged during full turning, and the autolysis phenomenon is avoided; improves the fluidity of the fermentation tank, and enables the culture solution at the bottom of the tank body to be mixed with the culture solution at other parts, thereby achieving the purposes of increasing the dissolved oxygen and homogenizing the oxygen.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural view of the first stirring assembly of the present invention.
FIG. 3 is a schematic diagram of the structure of the center plate of the present invention,
fig. 4 is a schematic view of the structure of the upper stirring blade of the present invention.
Fig. 5 is a schematic structural view of the second stirring assembly of the present invention.
Fig. 6 is a schematic view of the U-shaped frame structure of the present invention.
In the figure: 1-tank body, 2-closing plate, 3-tank cover, 4-support column, 5-feed inlet, 6-interface, 7-stirring device, 8-air distributor, 9-air tank, 10-discharge outlet, 12-transmission shaft, 13-bearing seat, 14-support frame, 15-first gear, 16-second gear, 17-first motor, 18-rolling bearing, 19-U-shaped frame, 20-driving rod, 21-second motor, 701-stirring shaft, 702-first stirring component, 703-central plate, 704-first stirring blade, 705-arc surface A, 706-arc surface B, 707-upper stirring blade, 708-lower stirring blade, 709-stirring blade A, 710-stirring blade B and 711-stirring fin, 712-auxiliary fins a, 713-auxiliary fins B, 714-second stirring assembly, 715-intermediate piece, 716-stirring sheet, 717-annular plate, 718-bottom plate.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description.
A plant cell culture fermentation tank comprises a tank body 1, a base, a tank cover 3, a stirring device 7, a supporting column 4, a first motor 17, a second motor 21 and a supporting frame 14;
the tank cover 3 and the sealing plate 2 are both made of stainless steel, the tank body 1 is made of glass, and the tank cover 3 is hermetically connected with the upper end face of the tank body 1; the sealing plate 2 is connected with the lower end face of the tank body 1, and the sealing plate 2 is fixed on the support column 4;
a feed inlet 5 is formed in the tank cover 3, a plurality of interfaces 6 are formed in the tank cover 3, the interfaces 6 are used for being connected with a pressure sensor, a temperature sensor and the like, the pressure sensor and the temperature sensor are electrically connected with a PLC (programmable logic controller), and the PLC is used for monitoring and adjusting culture conditions; the pressure sensor, the temperature sensor and the PLC are purchased parts and belong to the prior art, and the detailed description is omitted in the application;
the lower part of the tank body 1 is provided with a discharge hole 10; the bottom of the tank body 1 is provided with an air distributor 8, the air distributor 8 is connected with an air tank 9 through a pipeline, and the pipeline is provided with a valve;
the stirring device 7 is positioned in the tank body 1, and the stirring device 7 comprises a stirring shaft 701, a first stirring component 702 and a second stirring component 714;
the first stirring components 702 are uniformly distributed along the Z direction, and the first stirring components 702 comprise a central plate 703, first stirring blades 704 and stirring fins 711
The central plate 703 is fixed on the stirring shaft 701, and the central axis of the central plate 703 is superposed with the central axis of the stirring shaft 701; the central plate 703 comprises an arc surface a705 and an arc surface B706, the arc surface a705 and the arc surface B706 are vertically symmetrical, the arc surface a705 is positioned above the arc surface B706, the arc surface a705 and the arc surface B706 are connected into a whole, and the connection part is in arc transition;
the first stirring blades 704 are annularly arrayed around the central axis of the central plate 703, the first stirring blades 704 comprise upper stirring blades 707 and lower stirring blades 708, and the upper stirring blades 707 and the lower stirring blades 708 are vertically symmetrical about the central plate 703; the upper stirring blade 707 comprises a stirring blade A709 and a stirring blade B710, one end of the stirring blade A709 is connected with the arc surface A705, the other end of the stirring blade A709 is connected with the stirring blade B710 into a whole, the stirring blade A709 and the stirring blade B710 are vertically symmetrical, the stirring blade A709 is of an upwards-convex arc structure, and the section of the stirring blade A709 in the vertical plane direction is circular;
a plurality of stirring fins 711 are arranged between the upper stirring blade 707 and the lower stirring blade 708, and two ends of the stirring fins 711 are respectively connected with the upper stirring blade 707 and the lower stirring blade 708; the stirring fins 711 are of an outward convex arc structure, and the cross section of the stirring fins 711 in the horizontal plane direction is circular;
an auxiliary fin A712 is arranged above the upper stirring blade 707, the auxiliary fin A712 is of a circular arc structure, and two ends of the auxiliary fin A712 are respectively connected with the stirring blade B710;
an auxiliary fin B713 is arranged below the lower stirring blade 708, the auxiliary fin B713 is of an arc structure, and two ends of the auxiliary fin B713 are respectively connected with the stirring blade B710; the stirring blade B710 and the stirring blade A709 are vertically symmetrical;
the lower end of the stirring shaft 701 is connected with a second stirring component 714, and the second stirring component 714 is positioned below the first stirring component 702;
the second stirring assembly 714 comprises an intermediate piece 715 and stirring blades 716, wherein the intermediate piece 715 is fixed on the stirring shaft 701, the intermediate piece 715 comprises an annular plate 717 and a bottom plate 718, the annular plate 717 is of a concave annular structure, and the inner diameter of the annular plate 717 is gradually increased from top to bottom along the Z direction; the lower end surface of the annular plate 717 is connected with the bottom plate 718, and the connection part is in arc transition; the annular plate 717 is provided with a plurality of stirring blades 716, the stirring blades 716 are annularly arrayed around the central axis of the annular plate 717, the stirring blades 716 are of an outwards convex arc structure, and the section of the stirring blades 716 in the horizontal plane direction is circular;
the stirring device 7 can increase the dissolved oxygen and improve the fluidity; the first stirring component 702 and the second stirring component 714 which are in the shape of circular arcs are used for reducing shearing force, so that plant cells are prevented from autolysis; the auxiliary fins A712, the auxiliary fins B713 and the stirring sheets 716 are utilized to increase the stirring area, so that the full oxygen dissolution can be ensured at a low rotating speed, and the full fermentation of the plant cell culture solution is ensured;
the upper end of the stirring shaft 701 penetrates through the tank cover 3 to be connected with the transmission shaft 12, the stirring shaft 701 is rotatably connected with the tank cover 3, and the connection part is mechanically sealed; one end of the transmission shaft 12, which is far away from the stirring shaft 701, is rotatably connected with a bearing block 13, the bearing block 13 is fixed on a support frame 14, and the support frame 14 is fixed on the tank cover 3;
a first gear 15 is connected to the transmission shaft 12, the first gear 15 is meshed with a second gear 16, the second gear 16 is fixed on a first motor 17 shaft, and the first motor 17 is fixed on the support frame 14;
a rolling bearing 18 is connected to the transmission shaft 12, the rolling bearing 18 is located below the first gear 15, a U-shaped frame 19 is connected to the rolling bearing 18, a driving rod 20 is connected to one end, far away from the rolling bearing 18, of the U-shaped frame 19, the driving rod 20 is connected with a second motor 21 in a shaft mode, and the second motor 21 is fixed to the support frame 14;
the first motor 17 and the second motor 21 are both stepping motors; the second motor 21 is electrically connected with the power switch;
the second motor 21 is electrically connected with a stepping motor driver, the stepping motor driver is electrically connected with a controller, and the controller is used for controlling the second motor 21 to rotate for a certain angle each time; the second motor 21, the stepping motor driver and the controller are purchased parts, the first motor, the second motor and the stepping motor driver are Rasai brands, the controller brand is Siemens, specific structures of the controller are not repeated in the application, and the controller belongs to the prior art;
the first motor 17, the second motor 21, the first gear 15, the second gear 16 and the like form a driving structure of the stirring device 7; the first motor 17 is used for driving the second gear 16 to rotate, the second gear 16 is meshed with the first gear 15, and the stirring shaft 701 rotates, so that the first stirring component 702 and the second stirring component 714 stir the plant cell sap in the fermentation tank, namely the first motor 17 has the function of completing the rotating motion of the stirring device 7; the rotation of the second motor 21 is utilized to drive the transmission shaft 12 to realize reciprocating motion in the Z direction, the second motor 21 rotates anticlockwise for a certain angle to enable the transmission shaft 12 to rise linearly in the Z direction, and then the second motor 21 rotates clockwise for a certain angle to enable the transmission shaft 12 to fall linearly in the Z direction and return to the original height;
the transmission shaft can continue to engage the first gear 15 with the second gear 16 while moving, i.e. the second motor 21 functions to complete the linear movement of the stirring device 7; therefore, the stirring device 7 can turn over the plant cell culture solution in the fermentation tank in the ascending or descending process, and the structure of the intermediate piece 715 is utilized to ensure that the plant cell wall is not damaged when the plant cell culture solution is fully turned over, so that the autolysis phenomenon is avoided; the fluidity of the fermentation tank is improved, and the culture solution at the bottom of the tank body 1 is mixed with the culture solution at other parts, so that the aims of increasing the dissolved oxygen and homogenizing oxygen are fulfilled;
many other changes and modifications can be made without departing from the spirit and scope of the invention. It is to be understood that the invention is not to be limited to the specific embodiments, and that the scope of the invention is defined by the appended claims.
Claims (4)
1. A plant cell culture fermentation tank is characterized by comprising a tank body, a base, a tank cover, a stirring device and a supporting table;
the tank cover and the sealing plate are made of stainless steel, the tank body is made of glass, and the tank cover is hermetically connected with the upper end face of the tank body; the sealing plate is connected with the lower end face of the tank body;
the tank cover is provided with a feed inlet, and the tank inlet cover is provided with a plurality of connectors; a discharge hole is formed in the lower part of the tank body; the bottom of the tank body is provided with an air distributor, the air distributor is connected with the air tank through a pipeline, and the pipeline is provided with a valve;
the stirring device is positioned in the tank body and comprises a stirring shaft, a first stirring assembly and a second stirring assembly;
the first stirring components are uniformly distributed along the Z direction and comprise a central plate, first stirring blades and stirring fins
The central plate is fixed on the stirring shaft, and the central axis of the central plate is superposed with the central axis of the stirring shaft; the central plate comprises an arc surface A and an arc surface B, the arc surface A and the arc surface B are vertically symmetrical, the arc surface A is positioned above the arc surface B, the arc surface A and the arc surface B are connected into a whole, and the joint is in arc transition;
the first stirring blades are annularly arrayed around the central axis of the central plate, each first stirring blade comprises an upper stirring blade and a lower stirring blade, and the upper stirring blades and the lower stirring blades are vertically symmetrical about the central plate; the upper stirring blade comprises a stirring blade A and a stirring blade B, one end of the stirring blade A is connected with the arc surface A, the other end of the stirring blade A is connected with the stirring blade B into a whole, the stirring blade A and the stirring blade B are vertically symmetrical, the stirring blade A is of an upwards-convex arc structure, and the section of the stirring blade A in the vertical plane direction is circular;
a plurality of stirring fins are arranged between the upper stirring blade and the lower stirring blade, and two ends of each stirring fin are respectively connected with the upper stirring blade and the lower stirring blade; the stirring fins are of an outward convex arc structure, and the cross sections of the stirring fins in the horizontal plane direction are circular;
an auxiliary fin A is arranged above the upper stirring blade, the auxiliary fin A is of an arc structure, and two ends of the auxiliary fin A are respectively connected with the stirring blade B;
an auxiliary fin B is arranged below the lower stirring blade, the auxiliary fin B is of a circular arc structure, and two ends of the auxiliary fin B are respectively connected with the stirring blade B; the stirring blade B and the stirring blade A are vertically symmetrical;
the lower end of the stirring shaft is connected with a second stirring component, and the second stirring component is positioned below the first stirring component.
2. The plant cell culture fermenter according to claim 1, wherein the second stirring member comprises an intermediate member and a stirring plate, the intermediate member is fixed on the stirring shaft, the intermediate member comprises a ring plate and a bottom plate, the ring plate is of a concave ring structure, and the inner diameter of the ring plate gradually increases from top to bottom along the Z direction; the lower end surface of the annular plate is connected with the bottom plate, and the connection part is in arc transition; the annular plate is provided with a plurality of stirring pieces, the stirring pieces surround the annular array of the central axis of the annular plate, the stirring pieces are of an outward convex arc structure, and the cross sections of the stirring pieces in the horizontal plane direction are circular.
3. The plant cell culture fermenter according to claim 1 or 2, wherein the upper end of the stirring shaft penetrates through the tank cover and is connected with the transmission shaft, the stirring shaft is rotatably connected with the tank cover, and the connection is mechanically sealed; one end of the transmission shaft, which is far away from the stirring shaft, is rotatably connected with a bearing seat, the bearing seat is fixed on a support frame, and the support frame is fixed on the tank cover;
the transmission shaft is connected with a first gear, the first gear is meshed with a second gear, the second gear is fixed on a first motor shaft, and the first motor is fixed on the support frame;
even there is antifriction bearing on the transmission shaft, and antifriction bearing is located first gear below, even has the U-shaped frame on the antifriction bearing, and the one end that antifriction bearing was kept away from to the U-shaped frame even has the actuating lever, and the actuating lever is connected with second motor shaft, and the second motor is fixed on the support frame.
4. The plant cell culture fermentor of claim 3, wherein said first motor and said second motor are both stepper motors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920629715.1U CN210176875U (en) | 2019-05-06 | 2019-05-06 | Plant cell culture fermentation cylinder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920629715.1U CN210176875U (en) | 2019-05-06 | 2019-05-06 | Plant cell culture fermentation cylinder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210176875U true CN210176875U (en) | 2020-03-24 |
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ID=69832382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920629715.1U Expired - Fee Related CN210176875U (en) | 2019-05-06 | 2019-05-06 | Plant cell culture fermentation cylinder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210176875U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112617029A (en) * | 2020-12-16 | 2021-04-09 | 贵州金农富平生态农牧科技有限公司 | Preparation formula and preparation device of beef cattle feed |
-
2019
- 2019-05-06 CN CN201920629715.1U patent/CN210176875U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112617029A (en) * | 2020-12-16 | 2021-04-09 | 贵州金农富平生态农牧科技有限公司 | Preparation formula and preparation device of beef cattle feed |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200324 Termination date: 20210506 |