CN1168224A - Cultivation method of edible chinese holly leaf and its application - Google Patents

Cultivation method of edible chinese holly leaf and its application Download PDF

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CN1168224A
CN1168224A CN97109124A CN97109124A CN1168224A CN 1168224 A CN1168224 A CN 1168224A CN 97109124 A CN97109124 A CN 97109124A CN 97109124 A CN97109124 A CN 97109124A CN 1168224 A CN1168224 A CN 1168224A
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leaf
biologically active
powdered protein
active powdered
holly leaf
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CN1091569C (en
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陈华
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Abstract

Chinese holly branches grown for two years are cut, soaked with liquid of active protein powder to promote rooting, planted in March, transplanted in November and sprayed with active protein liquid during growth period. Chinese holly cultivated based on the said method grows rapidly, and its leaf has high amino acid content and comprehensive nutrient components. Chinese holly leaf after fermentation and hydrolysis is easy to absorb and its saccharified calcium is favourable to the growth of animal bone, so that it is used as feed for pig and chicken and as material for health food.

Description

The breeding method of edible holly leaf and the Preliminary Applications of leaf
The present invention relates to a kind ofly holly leaf be cultivated into the method for edible tree and leaf as food, the Preliminary Applications of feed.
Along with Developing of Animal Industry, the grain demand amount also increases thereupon, has been very urgent same topic with green grass, leaf, rhizome as feed how.
Chinese Pharmacopoeia indicates for 204 pages can make the dried leaves that medicinal holly leaf is a holly plant, be rectangle like or square circle shape rectangle, idol has long oval, long 3-8cm, wide 1.5-4cm, the hard ratchet that most advanced and sophisticated tool is 3 pieces bigger, the 1 piece of recurvation in top, the truncate or wide wedge shape of base portion, two 1-3 piece of each tool ratchet sometimes, the edge is rolling still, and long ovaide leaf does not have ratchet.Upper surface yellow green or green and brown look, glossy, lower surface lark or celadon.Vein pinniform, petiole is shorter.Keratin is hard and thick.Odorless, the little hardship of leaf.Nature and flavor with return through: bitter, cool.Return liver, kidney channel.
Holly leaf is wild, well developed root system, cold-resistant drought-enduring, adaptability is strong.One strain holly leaf by the root crosswise development, can grow five to six roots of sensation new talent after 3 years, and trunk can grow to two meters high after 2 years, and the maternal plant result can breed, but mutability, and rarely found.
Holly leaf non-toxic and safe, protein content are higher, can reach more than 30%, have it is directly fed pigs after plucking among the people.But because it is the root system breeding, garden and scale are planted beyond example still, make the dispersion of looking, gather difficulty.In addition, repeatedly pluck leaf artificially, it is more lived, and reorganization, reproduction speed will slow down greatly, and the leaf nutrient content reduces.The primary protein content of plucking for the second time of ratio is low by about 5% after tested, increase along with plucking number of times, and the curved decline of content, value is also along with reduction.Therefore must consider to mend nitrogen for tree.As with benefit nitrogen such as inorganic nitrogen-sourced urea, though plant absorbs rapidly, can cause that P in soil H changes, the soil soda acid worsens and hardens, and both unfavorable growth is contaminated environment also.
Purpose of the present invention is intended to solve the plantation of holly leaf scale, and growing period is mended nitrogen and the reasonable problem of utilizing of leaf, and the Preliminary Applications of the breeding method and the leaf of edible holly leaf is provided.
The implementation of the object of the invention is that the breeding method of edible holly leaf adopts biologically active powdered protein to cultivate.Biologically active powdered protein is to pull making beating out after bat, silkworm chrysalis, earthworm are cleaned with the water that contains light-coloured vinegar, bleaching powder, and dope filtration removes slag, centrifugation, low temperature drying are pulverized resulting.Getting biologically active powdered protein, to be mixed with weight ratio be 1% solution, the holly leaf beta pruning is placed in one soaked 4 hours, then takes out cuttage.Spray one time 1% biologically active powdered protein liquid again to young plant after January, transplant after 7 months.
Replenish active nitrogen with biologically active powdered protein during the plant strain growth, every annual 15-20g that mends of plant.
The method of replenishing active nitrogen has two kinds: the one, and place root 30cm place as base manure in dry powder, the 2nd, be made into 25% spray solution, sprayed 5 times in 1 year, 0.07 hectare sprays 500ml at every turn.
The Preliminary Applications of edible holly leaf, leaf adopts feed supplement to make the yeast juice batchwise, the concrete practice is, with leaf shredder, be put in pond or the vat, biologically active powdered protein liquid with 0.5% soaks, liquid level floods for good, ferments to pull out or directly use after sweetening in 1-2 days or dry stored for future use.And feed supplement continuously ferments, and or else adds yeast in addition.
" with the biologically active powdered protein of silkworm chrysalis, bat, earthworm preparation " (publication number 1106628) that the present invention adopts, be with 1 of bat, 5 of earthworms, 1000 usefulness of silkworm chrysalis contain in the 500ml water of light-coloured vinegar 10g, bleaching powder 100g cleans, then clean with clear water, flush away bleaching powder etc. are pulled out, the product of making beating, filtering-depositing, drying, pulverizing gained.It has preserved the active substance in the raw material (active extract) well, and protein content is up to more than 80%.
With cuttage after the beta pruning of biologically active powdered protein liquid immersion holly leaf, 0.07 hectare can be inserted strain more than 20,000 2 thousand, sprays biologically active powdered protein liquid to young plant, and young plant survival rate 80-85% grew to the 80cm higher position and can transplant in 7 months.
Need after the transplanting to replenish organic nitrogen source with biologically active powdered protein, plant grows vigorous under the effect of organic nitrogen source, can grow to 2-3 rice in 2 years, can grow into forest in 3 years, can pluck after 1 year, mend organic nitrogen after the harvesting again, can grow young leaves this month, even and often pluck, the amino acid content of leaf can not be less than 20% yet.Only adopt this breeding method to cultivate holly leaf young plant A and wild holly leaf B, adopt this breeding method not only to cultivate young plant but mend the holly leaf C of organic nitrogen, amino acid content (8%) that it plucks leaf for the second time contrasts and sees Table 1.Table 1
The name of an article Aspartic acid Threonine Serine Glutamic acid Glycine Alanine Cystine Valine Methionine Isoleucine Leucine Tyrosine Phenyl alanine Lysine Histidine Arginine Proline Tryptophan Add up to
????A ??2.33 ??0.83 ??0.86 ??2.52 ??1.22 ??1.38 ??0.11 ??1.24 ??0.26 ??1.06 ??2.03 ??0.76 ??1.26 ??1.51 ??0.47 ??1.30 ??1.13 ??- ??20.54
????B ??1.79 ??0.80 ??0.85 ??2.58 ??1.02 ??1.13 ??0.07 ??0.96 ??0.21 ??0.71 ??1.56 ??0.54 ??0.88 ??1.10 ??0.30 ??0.99 ??0.58 ??- ??16.1
????C ??2.64 ??1.34 ??1.94 ??5.46 ??1.53 ??2.21 ??0.23 ??1.78 ??0.50 ??1.54 ??2.68 ??1.08 ??1.42 ??1.95 ??0.62 ??1.96 ??1.24 ??- ??30.1
Annotate: the test (except that other has the equal g% of content of standard) that table 1 and following relevant test are done by Ministry of Agriculture food quality supervision verification test center.The amino acid content of the holly leaf leaf of cultivating with this breeding method and nutrient component, calcium, micro-equal size see Table 2,3 respectively with main animals and plants content contrast.
Table 2
The name of an article Aspartic acid Threonine Serine Glutamic acid Glycine Alanine Cystine Valine Methionine Isoleucine Leucine Tyrosine Phenyl alanine Lysine Histidine Arginine Proline Tryptophan Add up to
This product 2.33 0.83 0.86 2.52 ????1.22 ????1.38 ????0.11 ????1.24 ????0.26 ??1.06 ????2.03 ????0.76 ??1.26 ????1.51 ????0.47 ????1.30 ????1.13 - ????20.54
Rice 0.09 0.12 0.21 0.73 ????0.23 ????0.26 ????- ????0.42 ????0.34 ??0?20 ????0.33 ????0.32 ??0.25 ????0.03 ????0.06 ????0.27 ????0.74 - ?????4.60
Corn 0.16 0.29 0.34 1.28 ????0.58 ????0.44 ????- ????0.52 ????0.66 ??0.30 ????0.31 ????0.50 ??0.17 ????0.26 ????0.03 ????0.53 ????1.82 - ?????8.19
Soya bean 0.53 0.86 0.81 1.15 ????0?79 ????0.54 ????- ????0.70 ????0.45 ??0.64 ????0.91 ????0.73 ??0.75 ????0.18 ????0.45 ????1.01 ????0.57 - ????11.43
Standard flour 0?17 0.21 0.39 0.56 ????0.44 ????1.01 ????- ????0.90 ????1.33 ??0.23 ????0.74 ????0.53 ??0.24 ????0.10 ????0.03 ????0?61 ????0.66 - ?????8.15
Milk powder 0.34 0.39 0.45 0.65 ????0.21 ????0?37 ????- ????0.44 ????0.37 ??0.28 ????0.48 ????0.47 ??0.40 ????0.36 ????0.33 ????0.37 ????0.70 - ?????6.61
Pumpkin 0.08 0.01 0.02 0.05 ????0.01 ????0.02 ????- ????0.02 ????0.01 ??0.01 ????0.02 ????0.02 ??0.02 ????0.02 ????- ????0.03 ????0.01 0.01 ?????0.35
Orange 0.02 - 0.01 0.02 ????0?01 ????0.01 ????- ????0.02 ????0.01 ??0?01 ????0.01 ????0.02 ??0.01 ????0.01 ????0.01 ????0.07 ????0.06 - ?????0.30
Egg 1.44 0.71 1.18 2.06 ????0.53 ????0.92 ????- ????1.03 ????0.54 ??0.90 ????1.46 ????0.77 ??0.86 ????1.09 ????0.27 ????0.92 ????0.63 - ????15.31
Freezing lean meat 1.90 1.04 0.86 2.61 ????1.00 ????1.39 ????- ????1.22 ????0.65 ??1.15 ????1.79 ????0.94 ??0.98 ????2.00 ????1.18 ????1.70 ????0.95 - ????21.36
Soft-shelled turtle 2.01 0.94 0.93 3.44 ????1.00 ????1.24 ????0.13 ????0.83 ????0.69 ??0.84 ????1.62 ????0.70 ??1.15 ????1.65 ????0.61 ????1.22 ????0.72 - ????19.72
Table 3
The name of an article Weight (g) Moisture content (%) Protein (g) Fat (g) Carbohydrate (g) Heat (kcal) (MJ) Dietary fiber (g) Ash content (g) Vent in the sides of a garment flavol (mg) Carotene (mg) Vitamin E (mg) Thiamine (mg) Vitamin b3 (mg) Niacin (mg) Ascorbic acid (mg) Calcium (mg) Phosphorus (mg) Magnesium (mg) Potassium (mg) Sodium (mg) Zinc (mg) Iron (mg) Copper (mg) Manganese (mg) Selenium (mg)
This product 100 13.0 32.54 3.47 ?42.27 ?340(1.43) ??1.20 7.52 ?0.03 18.35 26.92 ??0.09 ?0.23 ?3.63 0.70 2710 ?600 ?640 ?1970 210 2.18 11.26 1.71 7.04 36.8
Rice 100 13.5 8.4 0.7 ?76.6 ?346(1.45) ??- 0.8 ?- - - ??0.31 ?0.07 ?- - 9 ?139 ?44 ?109 3.3 2.70 2.40 0.56 1.27 2.40
Corn 100 16.4 11.1 3.80 ?67.3 ?346(1.46) ??- 1.4 ?- - - ??0.07 ?0.21 ?- - 19 ?291 ?113 ?343 1.9 1.90 2.70 0.34 0.62 4.50
Soya bean 100 11.3 36.0 13 ?34.6 ?399(1.67) ??- 5.1 ?- - 3.42 ??0.04 ?0.28 ?- - 182 ?644 ?215 ?1420 30.4 1.91 9.6 1.86 2.50 4.30
The accurate flour of material 100 13.8 10.6 20 ?71.4 ?346(1.45) ??1.40 0.8 ?- - - ??0.18 ?0.12 ?1.4 - 35 ?54 ?22 ?145 6.0 2.02 5.6 0.32 - 4.52
Strength flour 100 22 19.3 21.4 ?52.3 ?422(1.77) ??- 4.8 ?0.14 - 0.09 ??0.04 ?0.27 ?1.3 6.0 316 ?588 ?37 ?343 248 2.71 1.2 0.08 - 21.32
Pumpkin 100 91.5 1.1 0 ?5.8 ?28(0.12) ??1.0 0.6 ?0.13 0.75 - ??- ?0.08 ?0.4 20 12 ?29 ?6 ?103 - 0.15 - 0.17 0.09 -
Orange 100 87.9 0.8 0.1 ?10.6 ?47(0.2) ??0.3 0.3 ?0.07 0.18 0.02 ??0.03 ?0.03 ?0.2 29.0 73 ?16 ?20 ?120 7.6 0.20 3.10 0.22 - 0.66
Egg 100 73.6 12 12.1 ?1.40 ?163(0.68) ??- 0.9 ?0.17 - 1.80 ??0.06 ?0.52 ?1.1 - 50 ?199 ?8 ?98 120.5 1.22 1.80 0.09 - 8.77
Leng Dong Of-thin meat 100 72.4 20.2 14.9 ?- ?215(0.90) ??- 1.1 ?- - - ??0.77 ?0.04 ?20.0 - 6 ?205 ?21 ?276 38.5 1.04 0.3 0.02 - 3.73
Soft-shelled turtle 100 78.6 17.1 1.26 ?1.99 ?88(0.37) ??- 1.01 ?0.03 - 0.21 ??0.06 ?0.12 ?- - 30.4 ?193 ?21.7 ?246 86.6 3.36 2.52 0.57 0.06 -
Concrete vitamin content (mg/100g) sees Table 4
Table 4
Project name Content Project name Content
Prokeyvit ????0.56 Vitamin D D ????3.63
Cholecalciferol ????0.52 Vitamin B1 ????0.93
Vitamin C ????0.70 Vitamin B2 ????2.28
As seen from the table, its amino acid content of leaf of the holly leaf of cultivating with breeding method of the present invention is except that freezing lean meat, and protein content all is higher than several main animals and plants foods such as vitamin and microelement etc. except that soya bean, particularly calcic is the last good merchantable brand of replenishing the calcium up to 2.71g/100g.
Accompanying drawing is a holly leaf leaf photo.
Holly leaf leaf calorific value height with the inventive method cultivation, behind fed-batch fermentation, under the effect of changeing basic enzyme, its amino acid is free state and exists, and can accept for integral animal, and its corpus fibrosum is softening, calcareous saccharification, very favourable to the animal skeleton development growth, its acid sterol thing and active extract have antitoxin mechanism, especially there is fabulous inhibitory action in animal breath road and gastral cross-infection.
The holly leaf leaf of cultivating with breeding method of the present invention (B) sees Table 5 with wild holly leaf leaf (A) sweet amino acid content contrast after fermenting.
Table 5
The amino acid title Content (%) The amino acid title Content (%)
?????A ????B ??A ??B
Aspartic acid ????ASP ????1.79 ????2.64 Phenyl alanine ????PHE ??0.88 ??1.42
Threonine ????THR ????0.80 ????1.34 Lysine ????LYS ??1.10 ??1.95
Serine ????SER ????0.85 ????1.94 Histidine ????HIS ??0.30 ??0.62
Glutamic acid ????GLU ????2.58 ????5.46 Arginine ????ARG ??0.99 ??1.96
Glycine ????GLY ????1.02 ????1.53 Proline ????PRO ??0.58 ??1.24
Alanine ????ALA ????1.13 ????2.21 Tryptophan ????TRP ??--- ??---
Cystine ????CYS ????0.07 ????0.23 Taurine ????TAU ??--- ??---
Valine ????VAL ????0.96 ????1.78 Y-aminobutyric acid ????G-ABA ??--- ??---
Methionine ????MET ????0.21 ????0.50 Ornithine ????ORN ??--- ??---
Isoleucine ????ILE ????0.71 ????1.54 Hydroxy-proline ????HYPRO ??--- ??---
Leucine ????LEU ????1.56 ????2.68 Summation ??16.1 ??30.1
Tyrosine ????TYR ????0.54 ????1.08
As seen from the table, leaf is after fermentation, and its amino acid whose summation can be 30.1.
Be used to feed pigs after the leaf fermentation, can save 400 kilograms in thickness grain for every, increase income 500 yuan, pig gets fat soon, meat is good.General 20kg piglet is fed after eight months and can reach the 150-200 kilogram, than shortening cycle 2-3 month with grain or forage feed.Be used for feeding chicken, duck, two months in advance laying period,, laying rate improves more than 30%, and the incidence of disease descends more than 60%.
Because leaf protein content height, calorific value height, vitamin, micro-A wide selection of colours and designs and polypeptide, polysaccharide that the present invention cultivates, be that any fruit, vegetables etc. are incomparable, therefore be the optimum feed stock of making the green health product, can be used to produce all nutrition type concentrate, beverage, health protection tea, health food, also can be used to produce the nourishing type fat-reducing product, the goods of replenishing the calcium, cosmetics and other series of products.

Claims (3)

1, the breeding method of edible holly leaf, the employing biologically active powdered protein is cultivated, biologically active powdered protein is with bat, silkworm chrysalis, earthworm is with containing light-coloured vinegar, the water of bleaching powder is pulled making beating out after cleaning, dope filtration removes slag, centrifugation, low temperature drying is pulverized resulting, it is characterized in that getting biologically active powdered protein, to be mixed with weight ratio be 1% solution, the holly leaf beta pruning is placed in one soaked 4 hours, then take out cuttage, spray one time 1% biologically active powdered protein liquid again to young plant after January, transplant after 7 months
Replenish active nitrogen with biologically active powdered protein during the plant strain growth, every annual 15-20g that mends of plant.
2,, it is characterized in that the method for replenishing active nitrogen has two kinds according to the breeding method of claim 1: the one, place root 30cm place as base manure in dry powder, the 2nd, be made into 25% spray solution, sprayed 5 times in 1 year, 0.07 hectare sprays 500ml at every turn.
3, the Preliminary Applications of edible holly leaf leaf, it is characterized in that adopting the fed-batch fermentation method, the concrete practice is, with leaf shredder, be put in pond or the vat, make yeast juice with 0.5% biologically active powdered protein liquid and soak, liquid level floods for good, ferment and pull out or directly use after sweetening in 1-2 days or dry stored for future use, and feed supplement continuously ferments, or else add yeast in addition.
CN97109124A 1997-05-30 1997-05-30 Cultivation method of edible chinese holly leaf and its application Expired - Fee Related CN1091569C (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103810145A (en) * 2012-11-06 2014-05-21 德克萨斯仪器股份有限公司 Viterbi butterfly operations
CN103918868A (en) * 2014-04-24 2014-07-16 陈华 Method for preparing bioactive albumen powder from silkworm pupae, bats and earthworms
CN104068276A (en) * 2013-08-18 2014-10-01 申健 Production method of ilex cornuta granulated feed for rabbits
CN108496888A (en) * 2018-01-30 2018-09-07 广西东兰县隘洞拉社通达种养专业合作社 A kind of pig-breeding method improving live pig meat

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1068010A (en) * 1992-05-18 1993-01-20 张明绪 Raising apple saplings by hard branch cutting
CN1042389C (en) * 1994-11-02 1999-03-10 陈华 Biologically active powdered protein extracted from silkworm chrysalis, bat and earthworm

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103810145A (en) * 2012-11-06 2014-05-21 德克萨斯仪器股份有限公司 Viterbi butterfly operations
CN104068276A (en) * 2013-08-18 2014-10-01 申健 Production method of ilex cornuta granulated feed for rabbits
CN104068276B (en) * 2013-08-18 2016-08-31 南陵宝恒野生葛种植专业合作社 A kind of Ilex cornuta Lindl. rabbit granulated feed producing method
CN103918868A (en) * 2014-04-24 2014-07-16 陈华 Method for preparing bioactive albumen powder from silkworm pupae, bats and earthworms
CN108496888A (en) * 2018-01-30 2018-09-07 广西东兰县隘洞拉社通达种养专业合作社 A kind of pig-breeding method improving live pig meat

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