CN116735536A - Optical detection device and blood analyzer - Google Patents
Optical detection device and blood analyzer Download PDFInfo
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- CN116735536A CN116735536A CN202310960233.5A CN202310960233A CN116735536A CN 116735536 A CN116735536 A CN 116735536A CN 202310960233 A CN202310960233 A CN 202310960233A CN 116735536 A CN116735536 A CN 116735536A
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- 238000001514 detection method Methods 0.000 title claims abstract description 92
- 230000003287 optical effect Effects 0.000 title claims abstract description 64
- 210000004369 blood Anatomy 0.000 title claims abstract description 21
- 239000008280 blood Substances 0.000 title claims abstract description 21
- 238000012545 processing Methods 0.000 claims abstract description 117
- 210000004027 cell Anatomy 0.000 claims abstract description 42
- 230000003321 amplification Effects 0.000 claims abstract description 10
- 230000003834 intracellular effect Effects 0.000 claims abstract description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 10
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 8
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 8
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 8
- 230000007246 mechanism Effects 0.000 claims description 18
- 239000006185 dispersion Substances 0.000 claims description 9
- 238000007493 shaping process Methods 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 3
- 238000004159 blood analysis Methods 0.000 claims description 3
- 230000006872 improvement Effects 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims 1
- 238000000034 method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000002253 acid Substances 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 3
- 201000009310 astigmatism Diseases 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002310 reflectometry Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
Abstract
The application provides an optical detection device and a blood analyzer, which comprises a laser component, a flow chamber component, a front scattering collecting component, a lateral fluorescence collecting component, a lateral scattering collecting component, a gain adjusting component, a reflecting mirror component, a signal processing board and a controller, wherein the front scattering collecting component is arranged on the flow chamber component; the front scattering collecting component, the lateral fluorescence collecting component and the photoelectric detectors of the lateral scattering collecting component are all photodiodes; the reflecting mirror component reflects the large-angle scattered light and the fluorescent light to the lateral scattered collecting component and the lateral fluorescent light collecting component; the signal processing board is based on the gain adjusting component, and adjusts the gains of the electric signals respectively output by the front scattering collecting component for detecting the small-angle scattered light, the side scattering collecting component for detecting the large-angle scattered light and the side fluorescent component for detecting the fluorescent light so as to obtain the electric signals after gain amplification; the controller outputs cell volume detection data, intracellular nucleic acid content detection data, and cell surface complexity detection data based on the gain-amplified electrical signals. The device has lower cost and higher detection rate.
Description
Technical Field
The application relates to the technical field of in-vitro diagnosis, in particular to an optical detection device and a blood analyzer.
Background
The blood analyzer is an important detection instrument in clinical examination application of hospitals, and provides important data reference for diagnosis and treatment of diseases. The blood analyzer mainly measures the components in the blood by the laser scattering principle; wherein, the detection of the cell volume is carried out by low-angle scattering, the detection of the cell surface complexity is carried out by 90-degree scattering, and the detection of the intracellular acid content is carried out by 90-degree fluorescence.
Currently, the blood analyzers on the market are generally expensive, and small-sized clinics and small-sized medical centers are generally difficult to bear the price of the blood analyzers, so that the use requirements of the small-sized clinics cannot be met. The optical signal detector in the optical detection device of the existing blood analyzer mainly comprises devices such as an Avalanche Photodiode (APD) or a photomultiplier tube (PMT), and the avalanche photodiode and the photomultiplier tube are high in cost, are easily influenced by weak ambient light and are relatively large in influence of ambient temperature change on detection precision, and easily cause the problem of low accuracy of the detection effect of the blood analyzer.
Disclosure of Invention
The application aims to provide an optical detection device and a blood analyzer, which are used for solving the technical problems that the existing optical detection device is high in cost and easy to influence by environment, and the accuracy of detection effect is low.
In a first aspect, the present application provides an optical detection device, including a laser assembly, a flow chamber assembly, a front scattering collection assembly, a lateral fluorescence collection assembly, a lateral scattering collection assembly, a gain adjustment assembly, a mirror assembly, at least one signal processing board, and a controller;
the laser output by the laser component forms a first focus on the flow chamber component, and scattered light and fluorescence are formed by the laser after passing through the flow chamber component, wherein the scattered light comprises small-angle scattered light and large-angle scattered light; the small-angle scattered light reaches the front scattered collection assembly, the large-angle scattered light reaches the side scattered collection assembly, and the fluorescence reaches the side fluorescence collection assembly; the mirror assembly is disposed opposite either of the lateral fluorescence collection assembly and the lateral dispersion collection assembly, the mirror assembly for reflecting high angle scattered light excited via the flow cell assembly to the lateral dispersion collection assembly and/or reflecting fluorescence excited via the flow cell assembly to the lateral fluorescence collection assembly;
the front scattering collecting component, the lateral fluorescence collecting component and the photoelectric detectors of the lateral scattering collecting component are all photodiodes;
the gain adjusting component is connected with the at least one signal processing board, the front scattering collecting component, the side scattering collecting component and the side fluorescence collecting component; the at least one signal processing board is based on the gain adjusting component, and adjusts the gains of the electric signals respectively output by the front scattering collecting component for detecting the small-angle scattered light, the side scattering collecting component for detecting the large-angle scattered light and the side fluorescent component for detecting the fluorescence, so as to obtain the electric signals after gain amplification;
the controller outputs cell volume detection data, intracellular nucleic acid content detection data and cell surface complexity detection data based on the gain-amplified electrical signals.
Further, the gain adjusting component at least comprises an amplifying circuit, and the at least one signal processing board is used for adjusting the gains of the electric signals respectively output by the front scattering collecting component for detecting the small-angle scattered light, the side scattering collecting component for detecting the large-angle scattered light and the side fluorescent component for detecting the fluorescent light based on the amplifying circuit so as to obtain the electric signals after gain amplification.
Further, the laser assembly comprises a laser module and a cylindrical mirror mechanism, the laser module outputs laser, the cylindrical mirror mechanism performs shaping and then emits the laser to the flow chamber assembly, and the laser module comprises a laser and a collimating lens which are integrated integrally;
the laser characteristic wavelength of the laser is 633-642 nm;
and the light spots output after the shaping by the cylindrical mirror mechanism are smaller than or equal to 8mm.
Further, the at least one signal processing board includes: the first signal processing board, the second signal processing board and the third signal processing board are the same type signal processing boards or the same processing principle signal processing boards;
the optical detection device comprises a first signal processing circuit, a second signal processing circuit and a third signal processing circuit; the first signal processing board, the second signal processing board and the third signal processing board are respectively used for obtaining an electric signal after gain amplification and signal-to-noise ratio improvement through the first signal processing circuit, the second signal processing circuit, the third signal processing circuit and the gain adjusting component.
Further, the front scatter collection assembly includes: the first signal processing board is connected with the first photoelectric diode;
the small-angle scattered light forms a second focus at a straight stop, and the straight stop is used for blocking stray light in the small-angle scattered light;
the first photodiode converts the optical signal of the light scattered at the small angle into a first electric signal, and the first electric signal is subjected to signal processing by the first signal processing board to obtain the cell volume detection data.
Further, the optical detection device comprises a lateral focusing lens, the large-angle scattered light and the fluorescence light reach the dichroic mirror through the focusing of the lateral focusing lens, and the dichroic mirror reflects the large-angle scattered light to the lateral scattering collection assembly; the fluorescence is transmitted to the lateral fluorescence collection assembly via the dichroic mirror.
Further, the lateral fluorescence collection assembly includes: an aperture stop, a long pass filter, a second photodiode, and a second signal processing board;
at least a portion of the fluorescence excited by the flow cell assembly passes through the aperture stop to the long pass filter;
the long-pass filter filters at least part of fluorescence, and at least part of fluorescence filtered by the long-pass filter is transmitted to the second photodiode;
the other part of fluorescence excited by the flow chamber component is reflected by the reflecting mirror component along an original light path, and sequentially passes through the lateral focusing lens, the dichroic mirror, the aperture diaphragm and the long-pass filter to reach the second photodiode;
the second photodiode converts the fluorescent light signal into a second electric signal, and the second electric signal is subjected to signal processing by the second signal processing board to obtain the intracellular nucleic acid content detection data.
Further, the side scatter collection assembly includes: an aperture stop, a third photodiode, and a third signal processing board;
at least a portion of the large angle scattered light excited by the flow cell assembly is received by the third photodiode after passing through the aperture stop; the other part of large-angle scattered light excited by the flow chamber assembly is reflected along an original light path by the reflecting mirror assembly, sequentially passes through the lateral focusing lens, the dichroic mirror and the aperture diaphragm to reach the third photodiode, the third photodiode converts an optical signal of the large-angle scattered light into a third electric signal, and the third electric signal is subjected to signal processing by the third signal processing board to obtain the cell surface complexity detection data.
Further, the cylindrical mirror mechanism is two cylindrical mirrors which are mutually perpendicular.
In a second aspect, the present application further provides a blood analyzer, which includes the optical detection device according to the above embodiment, and the blood analysis is implemented by using the optical detection device.
The application provides an optical detection device, which comprises a laser component, a flow chamber component, a front scattering collecting component, a lateral fluorescence collecting component, a lateral scattering collecting component, a gain adjusting component, at least one signal processing board and a controller, wherein the front scattering collecting component is connected with the flow chamber component; the laser output by the laser component forms a first focus on the flow chamber component, and scattered light and fluorescence are formed by the laser after passing through the flow chamber component, wherein the scattered light comprises small-angle scattered light and large-angle scattered light; the small-angle scattered light reaches the front scattered collection assembly, the large-angle scattered light reaches the side scattered collection assembly, and the fluorescence reaches the side fluorescence collection assembly; the front scattering collecting assembly, the lateral fluorescence collecting assembly and the photoelectric detectors of the lateral scattering collecting assembly are all photodiodes; the gain adjusting component is connected with the at least one signal processing board, the front scattering collecting component, the side scattering collecting component and the side fluorescence collecting component; the at least one signal processing board is based on the gain adjusting component, and adjusts the gains of the electric signals respectively output by the front scattering collecting component for detecting the small-angle scattered light, the side scattering collecting component for detecting the large-angle scattered light and the side fluorescent component for detecting the fluorescence, so as to obtain the electric signals after gain amplification; the controller outputs cell volume detection data, intracellular nucleic acid content detection data and cell surface complexity detection data based on the gain-amplified electrical signals.
The optical detection device provided by the application has the advantages that the laser module is adopted in the front scattering collecting assembly to output nearly collimated laser, the photoelectric detectors of the front scattering collecting assembly, the lateral fluorescence collecting assembly and the lateral scattering collecting assembly are all photodiodes, the photodiodes are used as the photoelectric detectors to be matched with the corresponding gain adjusting assemblies, the cost of the photodiodes is only one tenth to one hundredth of that of an avalanche photodiode or a photomultiplier, the photodiodes are not easily influenced by the ambient temperature and the ambient light, the optical detection device is suitable for the operation environment under multiple scenes, the operation can be performed without a high-voltage power supply, and the adaptation requirement on a signal processing board is low. Through gain adjustment subassembly, even use the lower photodiode of cost, also can guarantee the rate of accuracy of detection effect. The application of the reflecting mirror component allows the lateral fluorescence collecting component and the lateral scattering collecting component to receive the enhanced optical signal intensity at a lower cost in consideration of the convenience of the optical path layout and the overall layout. In conclusion, the device cost of the photoelectric detector is greatly reduced by utilizing the photodiode, and the cost of setting a high-voltage power supply and the development cost of a signal processing board are further reduced, so that the technical problems that the existing optical detection device is high in cost and easy to be influenced by the environment are effectively solved, and the detection accuracy is ensured.
Correspondingly, the blood analyzer provided by the application also has the technical effects.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present application, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a diagram illustrating an optical path structure of an optical detection device according to an embodiment of the present application;
FIG. 2 is a schematic diagram of an optical path of an optical detection device according to an embodiment of the present application;
reference numerals: 1. a laser assembly; 11. a laser module; 12. a cylindrical mirror mechanism; 2. a flow cell assembly; 3. a front scatter collection assembly; 4. a lateral focusing lens; 5. a dichroic mirror; 6. a lateral fluorescence collection assembly; 7. a lateral dispersion collection assembly; 8. a mirror assembly; 9. a lateral collimating lens.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The terms "comprising" and "having" and any variations thereof, as used in the embodiments of the present application, are intended to cover non-exclusive inclusion. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those listed but may optionally include other steps or elements not listed or inherent to such process, method, article, or apparatus.
Currently, the blood analyzers on the market are generally expensive, and small-sized clinics and small-sized medical centers are generally difficult to bear the price of the blood analyzers, so that the use requirements of the small-sized clinics cannot be met. The existing blood analyzer is mainly made of devices such as Avalanche Photodiodes (APDs) or photomultiplier tubes (PMTs), and the avalanche photodiodes and photomultiplier tubes are high in cost, are easily influenced by weak ambient light and have large influence on detection accuracy due to ambient temperature change.
Therefore, the existing blood analyzer has the technical problems of high cost and environmental influence.
In order to solve the above problems, an embodiment of the present application provides an optical detection device.
Referring to fig. 1 and 2, an embodiment of the present application provides an optical detection device, which includes a laser assembly 1, a flow chamber assembly 2, a front scatter collecting assembly 3, a lateral fluorescence collecting assembly 6, a lateral scatter collecting assembly 7, a gain adjusting assembly (not shown), a mirror assembly 8, at least one signal processing board (not shown), and a controller (not shown); the laser output by the laser component 1 forms a first focus on the flow chamber component 2, and after passing through the flow chamber component 2, the laser forms scattered light and fluorescence, wherein the scattered light comprises small-angle scattered light and large-angle scattered light; the small-angle scattered light reaches the front scattered collection assembly 3, the large-angle scattered light reaches the side scattered collection assembly 7, and the fluorescence reaches the side fluorescence collection assembly 6; a mirror assembly 8 is disposed opposite either of the lateral fluorescence collection assembly 6 and the lateral dispersion collection assembly 7, the mirror assembly 8 being configured to reflect high angle scattered light excited via the flow cell assembly 2 to the lateral dispersion collection assembly 7 and/or to reflect fluorescence excited via the flow cell assembly 2 to the lateral fluorescence collection assembly 6; the photodetectors of the front scattering collecting component 3, the lateral fluorescence collecting component 6 and the lateral scattering collecting component 7 are all photodiodes; the gain adjusting component is connected with at least one signal processing board, the front scattering collecting component 3, the side scattering collecting component 7 and the side fluorescence collecting component 6; the at least one signal processing board is based on a gain adjusting component, and is used for adjusting the gains of the electric signals respectively output by the front scattering collecting component 3 for detecting the small-angle scattered light, the side scattering collecting component 7 for detecting the large-angle scattered light and the side fluorescent component 6 for detecting the fluorescent light so as to obtain the electric signals after gain amplification; the controller outputs cell volume detection data, intracellular nucleic acid content detection data, and cell surface complexity detection data based on the gain-amplified electrical signals.
The reflecting mirror component 8 reflects the large-angle scattered light and fluorescence excited by the flow chamber component 2 back along the original light path after being collimated by the lateral collimating lens 9. The mirror in the mirror assembly 8 may be a total reflection mirror, with a reflectivity of 100%, or may be a partial mirror, i.e. a partial mirror, and a partial lens, for example. The reflectivity of the mirror may be set accordingly based on the optical signal strength required by the collection assembly.
By adopting the optical detection device provided by the application, the laser module 11 is adopted in the front scattering collecting component 3 to output nearly collimated laser, the photodetectors of the front scattering collecting component 3, the lateral fluorescence collecting component 6 and the lateral scattering collecting component 7 are all photodiodes, the photodiodes are used as the photodetectors to be matched with corresponding gain adjusting components, the cost of the photodiodes is only one tenth to one hundredth of that of an avalanche photodiode or a photomultiplier, the photodiodes are not easily influenced by the ambient temperature and the ambient light, the optical detection device is suitable for the operation environment under multiple scenes, the operation can be performed without a high-voltage power supply, and the adaptation requirement on a signal processing board is low. Through gain adjustment subassembly, even use the lower photodiode of cost, also can guarantee the rate of accuracy of detection effect. The use of the mirror assembly 8 allows the lateral fluorescence collection assembly 6 and the lateral dispersion collection assembly 7 to receive enhanced optical signal strength at a reduced cost, taking into account the light path layout and overall layout convenience. In summary, the use of the photodiode to configure the mirror assembly 8 not only greatly reduces the device cost of the photodetector and even the whole machine, but also further reduces the cost of setting up the high-voltage power supply and the development cost of the signal processing board, thereby effectively solving the technical problems of high cost and easy environmental influence of the existing optical detection device, and ensuring the detection accuracy.
In an exemplary embodiment, the gain adjusting component at least comprises an amplifying circuit, and the at least one signal processing board adjusts the gains of the electric signals respectively output by the front scattering collecting component 3 for detecting the scattered light with a small angle, the side scattering collecting component 7 for detecting the scattered light with a large angle and the side fluorescent collecting component 6 for detecting the fluorescent light based on the amplifying circuit, so as to obtain the electric signals after gain amplification.
In an exemplary embodiment, at least one signal processing board includes: the first signal processing board, the second signal processing board and the third signal processing board are the same type signal processing boards or the same processing principle signal processing boards; the optical detection device comprises a first signal processing circuit, a second signal processing circuit and a third signal processing circuit; the first signal processing board, the second signal processing board and the third signal processing board respectively obtain an electric signal after gain amplification and signal-to-noise ratio improvement through the first signal processing circuit, the second signal processing circuit, the third signal processing circuit and the gain adjusting component.
In one possible embodiment, the first signal processing board, the second signal processing board and the third signal processing board are the same type of signal processing board. Because the photoelectric detectors of the front scattering collecting assembly 3, the lateral fluorescence collecting assembly 6 and the lateral scattering collecting assembly 7 are all photodiodes, the signal processing boards of the three devices can be set to be the same type of signal processing boards, so that gain adjustment is only carried out on the same type of signal processing boards to meet the signal acquisition and processing of the front scattering, lateral and fluorescence three channels, the corresponding control boards and signal processing boards do not need to be independently developed aiming at different photoelectric detectors, and the production cost of the optical detection device is further reduced; meanwhile, the control convenience and consistency of the optical detection device are improved, errors among different types of optical signal processing are reduced, the accuracy of detection results is further improved, and mutual correction of different types of optical signal processing data is facilitated.
As shown in fig. 1, includes a laser assembly 1, a flow cell assembly 2, a front scatter collection assembly 3, a lateral focusing lens 4, a dichroic mirror 5, a lateral fluorescence collection assembly 6, and a lateral scatter collection assembly 7; the laser output by the laser component 1 forms a first focus on the flow chamber component 2, and the flow chamber component 2 provides a laser area after blood cells are sequentially shaped, wherein the first focus is formed in a direction perpendicular to the sample flow; laser light forms scattered light and fluorescence light via the flow cell assembly 2, the scattered light including small angle scattered light and large angle scattered light; the small-angle scattered light reaches the front scattered collection assembly 3, and the large-angle scattered light and fluorescence sequentially pass through the lateral focusing lens 4 and the dichroic mirror 5 and then respectively reach the lateral fluorescence collection assembly 6 and the lateral scattered collection assembly 7; the front scatter collection assembly 3, the lateral fluorescence collection assembly 6 and the lateral scatter collection assembly 7 respectively output cell volume detection data, intracellular nucleic acid content detection data and cell surface complexity detection data; the laser assembly 1 comprises a laser module 11 and a cylindrical mirror mechanism 12, wherein the laser module 11 outputs laser, and the laser is shaped by the cylindrical mirror mechanism 12 and then is emitted to the flow chamber assembly 2; the photodetectors of the front scatter collection assembly 3, the lateral fluorescence collection assembly 6 and the lateral scatter collection assembly 7 are photodiodes.
In the exemplary embodiment, the laser assembly 1 includes a laser module 11 and a cylindrical mirror mechanism 12, the laser module 11 outputs laser light, and the laser module 11 is shaped by the cylindrical mirror mechanism 12 and then emits the laser light to the flow cell assembly 2, and the laser film assembly 11 includes an integrated laser and collimator lens.
By adopting the optical detection device provided by the embodiment of the application, the laser module 11 and the cylindrical mirror mechanism 12 are adopted in the laser component 1, the laser module 11 is utilized to output nearly collimated laser, and the cylindrical mirror mechanism 12 is utilized to shape the laser, so that the laser meeting the laser output requirement is provided, the laser module 11 replaces the original Laser Diode (LD) to output the laser in a mode of combining with an aspheric collimating lens, the practical cost of components of the aspheric collimating lens and the operation links of lens installation and debugging are saved, and the production cost of the optical detection device is reduced. In addition, the photoelectric detectors of the front scattering collecting assembly 3, the lateral fluorescence collecting assembly 6 and the lateral scattering collecting assembly 7 are all photodiodes, the photodiodes are used as the photoelectric detectors, the cost of the photodiodes is only one tenth to one hundredth of that of an avalanche photodiode or a photomultiplier, the photodiodes are not easily affected by the ambient temperature and the ambient light, the photoelectric detector is suitable for the working environment under multiple scenes, the photoelectric detector can work without a high-voltage power supply, and the adaptation requirement on a signal processing board is low. Therefore, the device cost of the photoelectric detector is greatly reduced by utilizing the photodiode, and the cost of setting a high-voltage power supply and the development cost of a signal processing board are further reduced, so that the technical problems that the existing optical detection device is high in cost and easy to influence by the environment are effectively solved.
The laser characteristic wavelength of the laser in the laser module 11 is 633-642 nm; the light spot output after shaping by the cylindrical mirror mechanism 12 is less than or equal to 8mm. The collimation degree of the collimation lens of the laser module 11 is less than or equal to 1 deg.. In the embodiment, the setting of the characteristic wavelength of the laser, the collimation degree of the collimation lens and the matching cylindrical mirror mechanism 12 can enable the light spot output by the shaped laser to be smaller than or equal to 8mm; the stability of the transmitted laser light is better, and the laser light can irradiate cells more uniformly.
In one possible embodiment, the front scatter collection assembly 3 comprises: the device comprises a straight stop, a first photodiode and a first signal processing board; forming a second focus on the position of the straight stop by the laser, and blocking by the straight stop; the first photodiode converts an optical signal of the light scattered at a small angle into a first electrical signal, and the first electrical signal is subjected to signal processing by the first signal processing board to obtain cell volume detection data. The laser output by the laser component 1 interacts with the cells in the flow chamber component 2 to form scattered light, and part of laser which does not interact with the cells (namely leaked laser) is blocked by the straight stop, so that the leaked laser is prevented from reaching the first photodiode and interfering the signal acquisition of the first photodiode on the scattered light with a small angle. That is, the front scattering collecting assembly 3 blocks the leaked laser through the straight stop, receives the optical signal of the scattered light with a small angle through the first photodiode, converts the optical signal into a first electrical signal, and then processes the first electrical signal through the first signal processing board, so that cell volume detection data is obtained. The first photodiode and the first signal processing board are used for optical signal processing, so that the production cost of the front scattering collecting assembly 3 is reduced to the maximum.
In a possible embodiment, the optical detection means comprise a lateral focusing lens 4. The large angle scattered light and the fluorescence light pass through the focusing of the side focusing lens 4 to reach the dichroic mirror 5, and the dichroic mirror 5 divides the focused light into the large angle scattered light deflected by 90 degrees and the fluorescence light along the original light path, and the fluorescence light and the large angle scattered light reach the side fluorescence collection assembly 6 and the side scattering collection assembly 7, respectively. The dichroic mirror 5 is provided to separate the focused light into large-angle scattered light, i.e. lateral fluorescence reaching the lateral fluorescence collection assembly 6 and lateral astigmatism (in the same band as the excitation light), reaching the lateral astigmatism collection assembly 7, by the dichroic mirror 5.
In one possible embodiment, the lateral fluorescence collection assembly 6 comprises: an aperture stop, a long pass filter, a second photodiode, and a second signal processing board; at least a portion of the fluorescence excited by the flow cell assembly 2 passes through the aperture stop to the long pass filter; the long-pass filter filters at least part of fluorescence, and at least part of fluorescence filtered by the long-pass filter is transmitted to the second photodiode; the other part of fluorescence excited by the flow chamber component is reflected along an original light path by the reflecting mirror component 8, and sequentially passes through the lateral focusing lens 4, the dichroic mirror 5, the aperture diaphragm and the long-pass filter to reach the second photodiode;
the second photodiode converts the fluorescent light signal into a second electric signal, and the second electric signal is subjected to signal processing by the second signal processing board to obtain intracellular acid content detection data. The long-pass filter has the functions of fluorescence passing and other light cutting off, so that the focused light passing through the aperture diaphragm keeps fluorescence passing under the function of the long-pass filter, a second photodiode converts a fluorescence signal into a second electric signal, a second signal processing plate processes the second electric signal to obtain cell inner core acid content detection data, and the second photodiode and the second signal processing plate are arranged, so that the production cost of the lateral fluorescence collection assembly 6 is reduced maximally.
In one possible embodiment, the lateral dispersion collection assembly 7 comprises: an aperture stop, a third photodiode, and a third signal processing board; at least a portion of the large angle scattered light excited by the flow cell assembly 2 passes through the aperture stop and is received by the third photodiode; the other part of the large-angle scattered light excited by the flow chamber assembly 2 is reflected along the original light path by the reflecting mirror assembly 8, sequentially passes through the lateral focusing lens 4, the dichroic mirror 5 and the aperture diaphragm to reach the third photodiode, the third photodiode converts the optical signal of the large-angle scattered light into a third electric signal, and the third electric signal is subjected to signal processing by the third signal processing board, so that the cell surface complexity detection data is obtained. The side scattering collection component 7 receives the optical signals of the large-angle scattered light through the aperture diaphragm and the third photodiode, and processes the electrical signals of the large-angle scattered light through the third signal processing board, so that cell volume detection data are obtained. The third photodiode and the third signal processing board are utilized for optical signal processing, so that the production cost of the lateral scattering collecting component 7 is reduced to the greatest extent.
In one possible embodiment, the lateral focusing lens 4 is an aspherical lens arranged to focus lateral fluorescence and lateral astigmatism (i.e. large angle scattered light).
In one possible embodiment, the cylindrical mirror mechanism 12 is two cylindrical mirrors disposed perpendicular to each other. Two cylindrical mirrors perpendicular to each other are arranged to focus the nearly collimated laser output by the laser module 11, so that the laser forms a first focus perpendicular to the direction of the sample flow at the flow chamber assembly 2.
The embodiment of the application also provides a blood analyzer, which comprises the optical detection device provided by the embodiment, and the blood analyzer adopting the optical detection device has the advantages of lower cost and high detection stability, and meets the blood analysis and detection requirements of small-sized diagnostic departments and small-sized medical centers.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present application and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present application, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
Finally, it should be noted that: the above examples are only specific embodiments of the present application, and are not intended to limit the scope of the present application, but it should be understood by those skilled in the art that the present application is not limited thereto, and that the present application is described in detail with reference to the foregoing examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit of the corresponding technical solutions. Are intended to be encompassed within the scope of the present application. Therefore, the protection scope of the application is subject to the protection scope of the claims.
Claims (10)
1. An optical detection device is characterized by comprising a laser component, a flow chamber component, a front scattering collecting component, a lateral fluorescence collecting component, a lateral scattering collecting component, a gain adjusting component, a reflecting mirror component, at least one signal processing board and a controller;
the laser output by the laser component forms a first focus on the flow chamber component, and scattered light and fluorescence are formed by the laser after passing through the flow chamber component, wherein the scattered light comprises small-angle scattered light and large-angle scattered light; the small-angle scattered light reaches the front scattered collection assembly, the large-angle scattered light reaches the side scattered collection assembly, and the fluorescence reaches the side fluorescence collection assembly; the mirror assembly is disposed opposite either of the lateral fluorescence collection assembly and the lateral dispersion collection assembly, the mirror assembly for reflecting high angle scattered light excited via the flow cell assembly to the lateral dispersion collection assembly and/or reflecting fluorescence excited via the flow cell assembly to the lateral fluorescence collection assembly;
the front scattering collecting component, the lateral fluorescence collecting component and the photoelectric detectors of the lateral scattering collecting component are all photodiodes;
the gain adjusting component is connected with the at least one signal processing board, the front scattering collecting component, the side scattering collecting component and the side fluorescence collecting component; the at least one signal processing board is based on the gain adjusting component, and adjusts the gains of the electric signals respectively output by the front scattering collecting component for detecting the small-angle scattered light, the side scattering collecting component for detecting the large-angle scattered light and the side fluorescent component for detecting the fluorescence, so as to obtain the electric signals after gain amplification;
the controller outputs cell volume detection data, intracellular nucleic acid content detection data and cell surface complexity detection data based on the gain-amplified electrical signals.
2. The optical detection device according to claim 1, wherein the gain adjustment assembly comprises at least an amplifying circuit, and the at least one signal processing board adjusts the gain of the electrical signals respectively output by the front scattered light detection assembly detecting the small-angle scattered light, the side scattered light detection assembly detecting the large-angle scattered light and the side fluorescent light detection assembly based on the amplifying circuit, so as to obtain the electrical signals amplified by the gain.
3. The optical detection device according to claim 2, wherein the laser module comprises a laser module and a cylindrical mirror mechanism, the laser module outputs laser light, and the laser module is shaped by the cylindrical mirror mechanism and then emits the laser light to the flow chamber assembly, and the laser film module comprises a laser and a collimating lens which are integrated integrally;
the laser characteristic wavelength of the laser is 633-642 nm;
and the light spots output after the shaping by the cylindrical mirror mechanism are smaller than or equal to 8mm.
4. The optical detection device of claim 3, wherein the at least one signal processing board comprises: the first signal processing board, the second signal processing board and the third signal processing board are the same type signal processing boards or the same processing principle signal processing boards;
the optical detection device comprises a first signal processing circuit, a second signal processing circuit and a third signal processing circuit; the first signal processing board, the second signal processing board and the third signal processing board are respectively used for obtaining an electric signal after gain amplification and signal-to-noise ratio improvement through the first signal processing circuit, the second signal processing circuit, the third signal processing circuit and the gain adjusting component.
5. The optical detection device of claim 4, wherein the front scatter collection assembly comprises: the first signal processing board is connected with the first photoelectric diode;
the small-angle scattered light forms a second focus at a straight stop, and the straight stop is used for blocking stray light in the small-angle scattered light;
the first photodiode converts the optical signal of the light scattered at the small angle into a first electric signal, and the first electric signal is subjected to signal processing by the first signal processing board to obtain the cell volume detection data.
6. The optical detection device of any one of claims 1-4, wherein the optical detection device comprises a side focusing lens, the large angle scattered light and the fluorescent light passing through the focus of the side focusing lens to the dichroic mirror, the dichroic mirror reflecting the large angle scattered light to the side scatter collection assembly; the fluorescence is transmitted to the lateral fluorescence collection assembly via the dichroic mirror.
7. The optical detection device of claim 6, wherein the lateral fluorescence collection assembly comprises: an aperture stop, a long pass filter, a second photodiode, and a second signal processing board;
at least a portion of the fluorescence excited by the flow cell assembly passes through the aperture stop to the long pass filter;
the long-pass filter filters at least part of fluorescence, and at least part of fluorescence filtered by the long-pass filter is transmitted to the second photodiode;
the other part of fluorescence excited by the flow chamber component is reflected by the reflecting mirror component along an original light path, and sequentially passes through the lateral focusing lens, the dichroic mirror, the aperture diaphragm and the long-pass filter to reach the second photodiode;
the second photodiode converts the fluorescent light signal into a second electric signal, and the second electric signal is subjected to signal processing by the second signal processing board to obtain the intracellular nucleic acid content detection data.
8. The optical detection device of claim 6, wherein the side scatter collection assembly comprises: an aperture stop, a third photodiode, and a third signal processing board;
at least a portion of the large angle scattered light excited by the flow cell assembly is received by the third photodiode after passing through the aperture stop; the other part of large-angle scattered light excited by the flow chamber assembly is reflected along an original light path by the reflecting mirror assembly, sequentially passes through the lateral focusing lens, the dichroic mirror and the aperture diaphragm to reach the third photodiode, the third photodiode converts an optical signal of the large-angle scattered light into a third electric signal, and the third electric signal is subjected to signal processing by the third signal processing board to obtain the cell surface complexity detection data.
9. The optical inspection apparatus of claim 3 wherein said cylindrical mirror mechanism is two cylindrical mirrors disposed perpendicular to each other.
10. A blood analyser comprising an optical detection apparatus according to any one of claims 1 to 9 by which blood analysis is effected.
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| CN2022109374349 | 2022-08-05 | ||
| CN202210937434.9A CN115015178A (en) | 2022-08-05 | 2022-08-05 | Optical detection device and blood analyzer |
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| CN202322051612.0U Active CN220542782U (en) | 2022-08-05 | 2023-08-01 | Optical detection device and blood analyzer |
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| US5872627A (en) * | 1996-07-30 | 1999-02-16 | Bayer Corporation | Method and apparatus for detecting scattered light in an analytical instrument |
| US7978329B2 (en) * | 2000-08-02 | 2011-07-12 | Honeywell International Inc. | Portable scattering and fluorescence cytometer |
| US7580120B2 (en) * | 2005-04-07 | 2009-08-25 | Sysmex Corporation | Blood analyzer, sample analyzer, and flow cytometer |
| JP5259305B2 (en) * | 2007-10-03 | 2013-08-07 | シスメックス株式会社 | Cell analysis apparatus and cell analysis method |
| CN102095686A (en) * | 2010-12-21 | 2011-06-15 | 无锡荣兴科技有限公司 | Method for detecting and analyzing blue-green algae |
| CN202177574U (en) * | 2011-07-08 | 2012-03-28 | 无锡荣兴科技有限公司 | Photoelectric sensor for blood cell analysis |
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| CN208206768U (en) * | 2018-04-22 | 2018-12-07 | 利多(香港)有限公司 | A kind of optical system |
| WO2019206300A1 (en) * | 2018-04-28 | 2019-10-31 | 深圳迈瑞生物医疗电子股份有限公司 | Blood testing method and blood analysis system |
| CN209086141U (en) * | 2018-08-22 | 2019-07-09 | 桂林优利特医疗电子有限公司 | A kind of Optical devices for cell count and detection |
| CN109632742A (en) * | 2018-12-27 | 2019-04-16 | 苏州和迈精密仪器有限公司 | A kind of detection method of Resonance energy transfer fluorescence |
| CN110146430A (en) * | 2019-05-29 | 2019-08-20 | 中国科学院苏州生物医学工程技术研究所 | A kind of flow cytometer optical system |
| CN113125694B (en) * | 2019-12-31 | 2023-03-10 | 深圳市帝迈生物技术有限公司 | Detection system for realizing classification and quantitative analysis and detection method for immune multi-joint detection |
| CN212845402U (en) * | 2020-04-02 | 2021-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | Optical detection system for analyzing blood cells and blood cell analyzer |
| WO2022068630A1 (en) * | 2020-09-30 | 2022-04-07 | 深圳市帝迈生物技术有限公司 | Optical measurement apparatus and sample analyzer |
| CN112255166A (en) * | 2020-11-10 | 2021-01-22 | 中国科学院苏州生物医学工程技术研究所 | Scanning flow type cell imaging analyzer |
| CN114441480B (en) * | 2022-01-10 | 2024-01-26 | 中国科学院苏州生物医学工程技术研究所 | Nuclear erythrocyte analysis device and analysis method |
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