CN214334683U - Low-noise fluorescence detection sensor system - Google Patents
Low-noise fluorescence detection sensor system Download PDFInfo
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- CN214334683U CN214334683U CN202120476695.6U CN202120476695U CN214334683U CN 214334683 U CN214334683 U CN 214334683U CN 202120476695 U CN202120476695 U CN 202120476695U CN 214334683 U CN214334683 U CN 214334683U
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Abstract
A low-noise fluorescence detection sensor system relates to the technical field of detection and sensing. The optical fiber amplifier comprises a main body provided with at least two light through holes, wherein a circuit board is arranged on the upper side of the main body, a signal shielding protective cover and a circuit interface are arranged on the circuit board, and a trans-impedance amplifying circuit, a low-pass filter circuit, a secondary amplifying circuit, an analog-to-digital conversion circuit and a signal transmission circuit are arranged in the circuit board; the lower side of the circuit board is provided with a photoelectric detector, a receiving lens and a second optical filter; the light source is arranged in the collimating lens barrel, the collimating lens barrel is in threaded connection with one end of the light through hole of the excitation light path, the extinction screw thread is arranged in the light through hole of the excitation light path, and the extinction tail cone is arranged at the other end of the light through hole. The utility model discloses collect exciting light, light beam collimation, even light, extinction, fluorescence collection, fluorescence detection, filtering, analog-to-digital conversion, signal shielding protection in an organic whole, can provide fluorescence signal's digital quantity output, convenient direct links to each other with the computer, small, compact structure, commonality are strong, easily operation.
Description
Technical Field
The utility model relates to a detect and sensing technical field, in particular to reduce noise fluorescence detection sensor system.
Background
In recent years, with the rapid development of related technologies such as biotechnology, microfluidics technology, artificial intelligence, and the like, the demand for fluorescence-induced detection technology in fields such as nucleic acid PCR detection, protein analysis, medical analysis, analytical chemistry, food safety, water quality analysis, environmental detection, and the like has been rising.
The induced fluorescence method is a method for exciting a fluorescent substance in a sample by incident light to emit fluorescence and performing photoelectric conversion by a photoelectric detector, and has the advantages of high detection sensitivity, simplicity in operation and the like. The new generation of analysis and test instrument will be more miniaturized, portable and automatic.
At present, in the fluorescent detection technology in the market, the stray light extinction processing of a detection instrument is poor, the signal noise is high, the size is large, the structure is complex, the assembly difficulty is high, the maintenance and the repair are inconvenient, the price is high, and the requirement of rapid field test is difficult to meet.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a small, compact structure, the low noise fluorescence detection sensor system that the commonality is strong to prior art not enough.
In order to solve the technical problem, the following technical scheme is adopted in the application:
a low-noise fluorescence detection sensor system comprises a main body, wherein the main body is provided with a first light through hole and a second light through hole which are crossed in a cross manner; one end of the first light through hole is provided with a light source, and the other end of the first light through hole is provided with an extinction tail cone; and an extinction thread is arranged in the first light through hole.
The light source emitting end of the collimating lens barrel is in threaded connection with the first end of the first light through hole; and a dichroic mirror is obliquely arranged at the intersection of the first light through hole and the second light through hole, and the dichroic mirror (9) is used for reflecting light rays emitted by the light source (5) towards the first end direction of the second light through hole and allowing incident light from the first end direction of the second light through hole to pass through.
Further, the light source is arranged on a light source seat, and the light source seat is in threaded connection with the collimation lens cone.
Further, a light homogenizing sheet and a first light filter are arranged between the dichroic mirror and the collimating mirror group in the first light through hole.
Furthermore, a circuit board is arranged on the main body in the direction of the second end of the second light through hole, a signal shielding protective cover is arranged on the circuit board, and a photoelectric detector is arranged on the circuit board and matched with the second end of the second light through hole.
Further, the second light passing hole is provided with a second optical filter and a receiving lens between the dichroic mirror and the photodetector.
Further, the extinction tail cone is in threaded connection with the second end of the first light through hole.
Further, the photodetector is one or more of a PD photodiode, a PIN photodiode, an APD avalanche photodiode, an SiPM photodiode, a CMOS detector, and a CCD detector.
Compared with the prior art, the technical scheme of the application has the following beneficial effects:
1. the inner wall of the light through hole of the main body is provided with extinction threads, so that stray light of an excitation beam and ambient stray light can be fully eliminated;
2. the main body at the tail end of the excitation light path is provided with the extinction tail cone, so that redundant excitation light beams can be fully absorbed or deflected and scattered to the inner wall of the main body light through hole with the extinction threads, and redundant backscattered excitation light is prevented from entering a detector through the dichroic mirror, so that the optical noise of the sensor is reduced, and the detection sensitivity is improved;
3. the circuit board is provided with the low-pass filter circuit and the signal shielding protective cover, so that the interferences of power supply noise, signal jitter, electromagnetic radiation and the like can be fully filtered, and the detection accuracy is improved;
4. the circuit board is provided with an analog-to-digital conversion circuit, a signal transmission circuit and the like, can provide digital quantity output of a fluorescent signal, can be directly connected with a computer and is convenient to transmit to the computer for real-time data analysis;
5. the device integrates excitation light, light beam collimation, dodging, extinction, fluorescence collection, fluorescence detection, filtering and signal shielding protection, and has the advantages of small volume, compact structure, easy operation and low cost;
6. the structure is modularized, so that batch production, graded quality inspection and maintenance are facilitated;
drawings
FIG. 1 is a schematic cross-sectional view of the present invention
FIG. 2 is an exploded view of the structure of the present invention
FIG. 3 is a functional block diagram of the circuit of the present invention
In the figure, 1-main body; 2-a circuit board; 201-signal shielding protective cover; 202-a circuit interface; 3-light source seat; 4-a collimating lens barrel; 5-a light source; 6-a collimating lens group; 7-homogenizing a light sheet; 8-a first optical filter; 9-dichroic mirror; 10-extinction tail cone; 11-a second optical filter; 12-a receiving lens; 13-a photodetector; 14-side cover; 15-sample to be tested.
Detailed Description
The technical solution of the present application is further explained by the accompanying drawings and examples.
Referring to fig. 1 to 3, a low noise fluorescence detection sensor system in a preferred embodiment of the present application includes a main body 1, where the main body 1 is provided with a first light through hole and a second light through hole which are criss-cross; one end of the first light through hole is provided with a light source 5, and the other end of the first light through hole is provided with an extinction tail cone 10; and the inner wall of the first light through hole is provided with a light extinction thread.
The light source device further comprises a collimating lens barrel 4, a light source 5 and a collimating lens group 6 are arranged in the collimating lens barrel 4, and the light source emitting end of the collimating lens barrel 4 is in threaded connection with the first end of the first light through hole; a dichroic mirror 9 is obliquely arranged at the intersection of the first light through hole and the second light through hole, and a side cover 14 for fixing the dichroic mirror 9 in the cavity is further arranged; in the present embodiment, the dichroic mirror 9 is disposed to be inclined at 45 ° to the axes of the first and second light passing holes. Since the dichroic mirror operates on the principle that light of a certain wavelength is almost completely transmitted and light of other wavelengths is almost completely reflected, light of a specific wavelength can be separated from light of the light source and reflected toward the first end of the second through hole, and part of incident light from the first end of the second through hole can penetrate the dichroic mirror 9.
The light source 5 is arranged on the light source seat 3, and the light source seat 3 is in threaded connection with the collimating lens barrel 4; the first light through hole is provided with a light homogenizing sheet 7 and a first optical filter 8 between the dichroic mirror 9 and the collimating mirror group 6, wherein the light homogenizing sheet 7 plays a role of homogenizing light beams, and the first optical filter 8 is a narrow-band optical filter with a passband wavelength of 470 mm; the second light through hole is provided with a second optical filter 11 and a receiving lens 12 between the dichroic mirror 9 and the photoelectric detector 13, wherein the second optical filter 11 is a narrow-band optical filter, and the passband wavelength of the second optical filter is 525 mm; the receiving lens 12 plays a role in collecting weak fluorescence, so that the energy of the weak fluorescence is more concentrated, and the signal-to-noise ratio is improved.
A circuit board 2 is arranged on the main body in the direction of the second end of the second light through hole, a signal shielding protective cover 201 and a circuit interface 202 are arranged on the circuit board 2, and a photoelectric detector 13 is arranged on the circuit board 2 and the second end of the second light through hole in a matching manner; the circuit board 2 is internally provided with a transimpedance amplifier circuit, a low-pass filter circuit, a secondary amplifier circuit, an analog-to-digital conversion circuit and a signal transmission circuit, and is electrically connected with the circuit interface 202 on the circuit board 2, wherein the signal shielding protective cover 201 can filter electromagnetic radiation, so that the signal-to-noise ratio of the sensor system is improved.
The light beam emitted by the light source 5 passes through the collimating lens group 6 to obtain a more collimated light beam, the light spot of the light beam is further homogenized through the light homogenizing sheet 7, monochromatic light is formed through the narrow-band first light filter 8, and then the light beam is reflected through the dichroic mirror 9 which is set to be at an angle of 45 degrees and then irradiates on a sample 15 to be measured; wherein, when part of the stray light emitted by the light source 5 is not reflected to the sample 15 to be measured by the dichroic mirror 9, the stray light transmitted through the dichroic mirror 9 impinges on an extinction tail cone 10, which in this embodiment is a conical lens with a cone angle of 90 degrees 10, the inner wall of the lens can refract most incident light rays in the lens, the rest light rays can be transversely refracted by a large angle and irradiate on the side wall of the first light through hole near the extinction tail cone 10, extinction threads are arranged in the illumination range of the side wall of the first light through hole, that is, the light can be totally absorbed by repeated diffuse reflection, thereby preventing unnecessary stray light from irradiating the dichroic mirror 9 and then being reflected to the photoelectric detector 13 to form interference, after knowing the principle, the prism or other angle lens and the conical metal cone/plastic cone and other reflectors can be used as the extinction tail cone, and are not described in detail.
After being excited, the fluorescent substance in the sample 15 to be detected emits fluorescence, passes through the dichroic mirror 9, the second optical filter 11 and the receiving lens 12 to reach the photoelectric detector 13, and finally is connected with the electrical appliance of the circuit board 2 through the photoelectric detector 13 to complete the detection of the fluorescence. By matching the extinction tail cone 10 with the extinction thread, the system achieves a better extinction processing effect, reduces the optical noise of the sensor and improves the detection sensitivity.
As shown in fig. 2 to fig. 3, the transimpedance amplifier circuit is disposed on the circuit board 2 to convert the photocurrent of the photodetector 13 into an analog signal, and the low-pass filter circuit disposed on the circuit board 2 effectively filters the power noise, signal jitter, and other interferences, thereby improving the accuracy of detection. The circuit board 2 is provided with a secondary amplifying circuit for further amplifying the effective signals, and the amplified signals are divided into two paths: one path of analog quantity is directly and electrically connected with the circuit interface 202, so that a user can collect analog signals; the other path is converted into digital quantity through the arranged analog-to-digital conversion circuit and then is sent to the circuit interface 202 through a signal transmission circuit, wherein the signal transmission circuit is a serial port pin of a single chip such as STM32 and is realized by combining with TTL, RS232 or RS485 circuits.
The above description is only for the preferred embodiment of the present application, and is not intended to limit the technical scope of the present application in any way. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the concept of the present application, which falls within the scope of protection of the present application. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (8)
1. The low-noise fluorescence detection sensor system is characterized by comprising a main body (1), wherein the main body (1) is provided with a first light through hole and a second light through hole which are crossed in a cross manner; one end of the first light through hole is provided with a light source (5), and the other end of the first light through hole is provided with an extinction tail cone (10); and an extinction thread is arranged in the first light through hole.
2. The low-noise fluorescence detection sensor system according to claim 1, further comprising a collimating lens barrel (4), wherein a light source (5) and a collimating lens group (6) are disposed in the collimating lens barrel (4), and a light source emitting end of the collimating lens barrel (4) is in threaded connection with the first end of the first light passing hole; and a dichroic mirror (9) is obliquely arranged at the intersection of the first light through hole and the second light through hole, and the dichroic mirror (9) is used for reflecting light rays emitted by the light source (5) towards the first end direction of the second light through hole and allowing incident light from the first end direction of the second light through hole to pass through.
3. The low noise fluorescence detection sensor system of claim 2, wherein the light source (5) is disposed on a light source base (3), and the light source base (3) is screwed with the collimating lens barrel (4).
4. A low noise fluorescence detection sensor system according to claim 2, wherein the first light passing aperture is provided with a light homogenizing sheet (7) and a first filter (8) between the dichroic mirror (9) and the set of collimating mirrors (6).
5. A low noise fluorescence detection sensor system according to any of claims 2-4, wherein a circuit board (2) is arranged on the body (1) in the direction of the second end of the second through hole, a signal shielding protective cover (201) is arranged on the circuit board (2), and a photodetector (13) is arranged on the circuit board (2) in cooperation with the second end of the second through hole.
6. A low noise fluorescence detection sensor system according to claim 5, wherein the second light aperture is provided with a second optical filter (11) and a receiving lens (12) between the dichroic mirror (9) and the photodetector (13).
7. The low noise fluorescence detection sensor system of any of claims 2-4, wherein the extinction tail cone (10) is threadably connected to the second end of the first light passing hole.
8. The low noise fluorescence detection sensor system of claim 5, wherein said photodetector is one or more of a PD photodiode, a PIN photodiode, an APD avalanche photodiode, a SiPM photodiode, a CMOS detector, and a CCD detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120476695.6U CN214334683U (en) | 2021-03-05 | 2021-03-05 | Low-noise fluorescence detection sensor system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120476695.6U CN214334683U (en) | 2021-03-05 | 2021-03-05 | Low-noise fluorescence detection sensor system |
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| Publication Number | Publication Date |
|---|---|
| CN214334683U true CN214334683U (en) | 2021-10-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202120476695.6U Active CN214334683U (en) | 2021-03-05 | 2021-03-05 | Low-noise fluorescence detection sensor system |
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| CN (1) | CN214334683U (en) |
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2021
- 2021-03-05 CN CN202120476695.6U patent/CN214334683U/en active Active
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