CN110146430A - A kind of flow cytometer optical system - Google Patents
A kind of flow cytometer optical system Download PDFInfo
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- CN110146430A CN110146430A CN201910459771.XA CN201910459771A CN110146430A CN 110146430 A CN110146430 A CN 110146430A CN 201910459771 A CN201910459771 A CN 201910459771A CN 110146430 A CN110146430 A CN 110146430A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 166
- 238000001514 detection method Methods 0.000 claims abstract description 37
- 230000008054 signal transmission Effects 0.000 claims abstract description 30
- 239000000126 substance Substances 0.000 claims abstract description 28
- 239000013307 optical fiber Substances 0.000 claims abstract description 27
- 230000005284 excitation Effects 0.000 claims abstract description 21
- 230000003595 spectral effect Effects 0.000 claims abstract description 17
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims description 7
- 230000004888 barrier function Effects 0.000 claims description 5
- 239000000571 coke Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000005259 measurement Methods 0.000 abstract description 7
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000006185 dispersion Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N15/1436—Optical arrangements the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
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- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of flow cytometer optical systems, wherein the channel passed through for substance to be detected is provided in flow chamber；The excitation light source of light source module forms the focal beam spot not being overlapped in the channel；Light collection module exports after being assembled for collecting scattering light and/or fluorescence；Forward detection module is for receiving scattering light and detecting；Fluorescence detection module includes optical signal transmission module, spectral module and detecting module, mutually disjoint any position is arranged within the scope of the optical mirror slip full aperture in optical signal transmission module, optical signal for exporting light collection module deflects, optical signal after spectral module is used to deflect exports, and detecting module is for detecting optical signal.By applying the present invention, multiple photoelectric sensors and light-dividing device can be arranged in fluorescence detection module, the problem of causing measurement result to deteriorate or fail using optical fiber transmission Shi Yinguang collection module minute movement is overcome, the stability of system is enhanced.
Description
Technical field
The present invention relates to cell detection and analysis technical fields, and in particular to a kind of flow cytometer optical system.
Background technique
Flow cytometer be one kind can to cell or some particles (such as polystyrene microsphere) characteristic (such as size,
Refractive index, complexity of internal structure etc.) instrument quickly analyzed.Sample containing cell is focused by sheath hydraulic compression,
Laminar flow is formed into after in fluid pool, cell is compressed on sample streamline, singly by laser facula, uses detection
Device scattering light for generating and entrained when laser facula is passed through in the front of optical axis direction and the lateral test cell of optical axis
The specificity fluorescent that fluorescent dye generates, thus test cell or the biophysics of molecule, biochemical characteristic.
With the innovation of technology, the fluorescence channel number requirement that biological study and clinical diagnosis flow cytometric can be tested is more next
More, single Laser flow cytometry has been unable to meet their demand, it has been developed that the flow cytometer of multiple exciting lights,
So that the fluorescence channel number that flow cytometer is capable of measuring is more and more, the fluidic cell of existing multiple exciting light compositions
Instrument, in order to avoid fluorescence crosstalk, mostly using multiple (such as three) exciting light solid excitations greatly, (three light sources are formed in flow chamber
Three focuses be not to be overlapped, be separation), object lens cooperate optical fiber carry out optical signal collection, transmission scheme, but it is this
Scheme is limited to limited (the distance between three focuses usually only 80um to the 200um or so, it is assumed that use 40 times in spatial position
Object lens, apart from ability 3.2mm to 8mm or so), multiple photoelectric sensors and light-dividing device can not be placed to collect multiple laser and generate
Optical signal, the testing scheme of multiple exciting lights can not be adapted to.
Summary of the invention
In view of this, the embodiment of the invention provides a kind of flow cytometer optical system, it is existing multiple sharp to solve
The flow cytometer of composition of shining is limited to that spatial position is limited, can not place the technology of multiple photoelectric sensors and light-dividing device
Problem.
Technical solution proposed by the present invention is as follows:
The embodiment of the present invention provides a kind of flow cytometer optical system, which includes: flowing
Room, light source module, light collection module, forward detection module and fluorescence detection module；It is provided in the flow chamber for be detected
The channel that substance passes through, the channel are transmissive to optical signal；At least two excitation light sources are set in the light source module, it is described
Excitation light source forms the focal beam spot not being overlapped in the channel；At least a piece of optical mirror slip, institute are set in the smooth collection module
Scattering light and/or fluorescence that optical mirror slip is generated for collecting substance to be detected through excitation light source irradiation are stated, and to the scattering
Light and/or fluorescence export after being assembled；The forward detection module irradiates production through excitation light source for receiving substance to be detected
Raw scattering light, and the scattering light is detected, obtain the biophysical information of substance to be detected；The fluorescence detection mould
Block includes optical signal transmission module, spectral module and detecting module, and the optical signal transmission module is arranged in the optical mirror slip
Mutually disjoint any position within the scope of full aperture, for so that light collection module output optical signal deflect, described point
Optical module is used to export the optical signal after deflection according to specific wavelength, and the detecting module is used for the light exported to spectral module
Signal is detected, and biophysics, the biochemical information of substance to be detected are obtained.
Optionally, the optical signal transmission module includes: any one in reflecting mirror, reflecting prism or refracting prisms.
Optionally, the forward detection module includes: diaphragm, and the diaphragm is transferred to the forward detection mould for stopping
The optical signal of predetermined angle in block.
Optionally, the diaphragm includes: aperture and barrier rib, and the barrier rib is arranged in the aperture, for stopping
The optical signal of predetermined angle.
Optionally, the forward detection module includes: the first optical filter and forward detection device, and first optical filter is used for
It treats the scattering light that detection substance generates to be screened, the forward detection device detects the optical signal after screening.
Optionally, the spectral module includes: the second optical filter, and second optical filter is for the optical signal after deflecting
In fluorescence signal separation.
Optionally, the spectral module includes: the lens with positive light coke, and the detecting module includes that multiple photoelectricity are visited
Device is surveyed, the lens receive isolated optical signal and focusing forms hot spot, and the hot spot is less than the photosensitive area of photodetector.
Optionally, optics of the image-forming range of the optical mirror slip more than or equal to the optical mirror slip to photodetector
Length.
Optionally, the image space angular aperture of the optical mirror slip is not more than 5 degree.
Optionally, the optical signal transmission module further include: optical fiber and collimation lens, the collimation lens are arranged described
The both ends of optical fiber, the optical fiber are used for the received optical signal transmission of collimated lens into the spectral module.
Technical solution proposed by the present invention, has the advantages that
Flow cytometer optical system provided in an embodiment of the present invention, by the way that light is arranged in the fluorescence detection module of system
Signal transmission module, the optical signal for exporting to light collection module deflect, and the setting of optical signal transmission module is received in light
Collect mutually disjoint any position within the scope of module full aperture, the optical signal dispersion that light collection module can be made to export, thus
It realizes the separation of optical signal caused by different exciting lights, and multiple photoelectric sensors and light-dividing device is set.Also, compared to existing
Have and directlys adopt multiple optical fiber collection multi beam optical signals in technology and be transmitted to the scheme detected in sensor, this hair
The flow cytometer optical system that bright embodiment provides, the light that light collection module is exported by the optical signal transmission module of setting
Signal is deflected, can make deflection after optical signal be split module reception, be not in directly adopt optical fiber collect when,
Due to fiber end face limitation so that optical path occur minute movement optical signal can not be collected by optical fiber, lead to the change of measurement result
The problem of different coefficient increases, therefore, flow cytometer optical system provided in an embodiment of the present invention, even if there is the small model of optical path
Drift is enclosed, the problem of optical signal can not be collected will not occur, so that the tolerable error of system increases, is overcome using optical fiber
The problem of transmission Shi Yinguang collection module minute movement causes measurement result to deteriorate or fail, enhance the stability of system.
In addition, the diaphragm being arranged in the flow cytometer optical system can stop the optical signal of predetermined angle, it will be without object to be detected
The optical excitation signal of matter scattering is separated, and is improved scattering light detection sensitivity, is overcome existing flow cytometer forward scattering light
The larger problem of detection noise.
Detailed description of the invention
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art be briefly described, it should be apparent that, it is described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the structural block diagram of flow cytometer optical system according to an embodiment of the present invention；
Fig. 2 is the structural block diagram of flow cytometer optical system according to another embodiment of the present invention；
Fig. 3 is the structural block diagram of flow cytometer optical system according to another embodiment of the present invention；
Fig. 4 is the structural block diagram of flow cytometer optical system according to another embodiment of the present invention.
Specific embodiment
Technical solution of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
Personnel's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " center ", "upper", "lower", "left", "right", "vertical",
The orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" be based on the orientation or positional relationship shown in the drawings, merely to
Convenient for description the present invention and simplify description, rather than the device or element of indication or suggestion meaning must have a particular orientation,
It is constructed and operated in a specific orientation, therefore is not considered as limiting the invention.In addition, term " first ", " second ",
" third " is used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also indirectly connected through an intermediary, it can be with
It is the connection inside two elements, can be wireless connection, be also possible to wired connection.For those of ordinary skill in the art
For, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As long as in addition, the non-structure each other of technical characteristic involved in invention described below different embodiments
It can be combined with each other at conflict.
The embodiment of the present invention provides a kind of flow cytometer optical system, as shown in Figure 1, the flow cytometer optical system
It include: flow chamber 2, light source module 3, light collection module 4, forward detection module 1 and fluorescence detection module 5；It is arranged in flow chamber 2
There is the channel passed through for substance to be detected, which is transmissive to optical signal；At least two excitation light sources are set in light source module 3
31 (being as shown in the figure three excitation light sources), excitation light source 31 forms the focal beam spot not being overlapped in the channel；Light collection module 4
The middle at least a piece of optical mirror slip of setting, optical mirror slip are used to collect the scattering light that substance to be detected is generated through excitation light source irradiation
And/or fluorescence, and exported after being assembled to scattering light and/or fluorescence；Forward detection module 1 is for receiving substance warp to be detected
The scattering light that excitation light source irradiation generates, and scattering light is detected, obtain the surface information of substance to be detected；Fluorescence detection
Module 5 includes optical signal transmission module 51, spectral module 52 and detecting module 53, and optical signal transmission module 51 is arranged in optical frames
Mutually disjoint any position within the scope of piece full aperture, for so that light collection module 4 export optical signal deflect, be divided
Module 52 is used to export the optical signal after deflection according to specific wavelength, and the light that detecting module 53 is used to export spectral module is believed
It number is detected, obtains the internal information of substance to be detected.
Flow cytometer optical system provided in an embodiment of the present invention, by the way that light is arranged in the fluorescence detection module of system
Signal transmission module, the optical signal for exporting to light collection module deflect, and the setting of optical signal transmission module is received in light
Collect mutually disjoint any position within the scope of module full aperture, the optical signal dispersion that light collection module can be made to export, thus
It realizes the separation of optical signal caused by different exciting lights, and multiple photoelectric sensors and light-dividing device is set.Also, compared to existing
Have and directlys adopt multiple optical fiber collection multi beam optical signals in technology and be transmitted to the scheme detected in sensor, this hair
The flow cytometer optical system that bright embodiment provides, the light that light collection module is exported by the optical signal transmission module of setting
Signal is deflected, can make deflection after optical signal be split module reception, be not in directly adopt optical fiber collect when,
Due to fiber end face limitation so that optical fiber occur minute movement optical signal can not be collected by optical fiber, lead to the change of measurement result
The problem of different coefficient increases, therefore, flow cytometer optical system provided in an embodiment of the present invention, even if optical signal transmission module
Minute movement occurs, the problem of optical signal can not be collected will not occur, so that the tolerable error of system increases, overcomes and adopts
The problem of causing measurement result to deteriorate or fail with optical fiber transmission Shi Yinguang collection module minute movement, enhance the steady of system
It is qualitative.
Specifically, in flow cytometer optical system provided in an embodiment of the present invention, the optical signal of the transmitting of excitation light source 31
The direction of propagation it is vertical with the flow direction of substance to be detected in flow chamber 2.Optionally, substance to be detected can be cell or micro-
Grain (such as polystyrene microsphere), is also possible to other substances, which is not limited by the present invention.It may include in excitation light source 31
The laser of tri- wavelength of 488nm, 405nm, 638nm.In addition, the center for the focal beam spot that excitation light source 31 is formed in the channel
Line is overlapped with the line of flow of substance to be detected, and the center line and line of flow can be at the centers in 2 channel of flow chamber.
In flow cytometer optical system provided in an embodiment of the present invention, light collection module 4 can be overall with positive
The object lens of focal power, the visual field of the object lens cover the focus of all excitation light sources 31 of light source module in the tiny channels, and to warp
Optical signal after substance to be detected is assembled, and the picture point of equal number is formed, or is imaged on unlimited distance.Specifically, the object
The image-forming range of mirror is greater than or equal to the optical length of object lens photodetector into detecting module.Specifically, the picture of the object lens
Square angular aperture is not more than 5 degree.Optionally, which can be selected the object lens that numerical aperture (NA) is greater than or equal to 0.6.
As a kind of optional embodiment of the embodiment of the present invention, optical signal transmission module 51 includes: reflecting mirror, reflection
Any one in prism or refracting prisms.The whole that the optical signal transmission module 51 can be such that optical signal collection module 4 exports
Optical signal deflects, and the angle of deflection is at 0 degree between 180 degree.
As a kind of optional embodiment of the embodiment of the present invention, as shown in Fig. 2, forward detection module 1 includes: diaphragm
11, microscope group 12, the first optical filter 13 and forward detection device 14.
Wherein, diaphragm 11 is used to stop to be transferred to the optical signal of predetermined angle in forward detection module 1, specifically, diaphragm
11 can stop the optical signal of 0 degree to 2 degree of optical signal detecting point.As shown in figure 3, diaphragm 11 can be by aperture and barrier rib structure
At aperture can be oval aperture, and barrier rib is arranged in aperture, for stopping the optical signal of predetermined angle.Diaphragm 11 exists
45 degree of placements can be tilted in the forward detection module 14, the clear aperature of the diaphragm 11 is circle, and angular aperture is 10 degree.This
Outside, when diaphragm 11 stops optical signal, the optical signal of predetermined angle can be made to turn 90 degrees partially, the optical signal after deflection can use light
Trap absorbs.The diaphragm 11 being arranged in the flow cytometer optical system can stop the optical signal of predetermined angle, will without to
The optical excitation signal of detection substance scattering is separated, and scattering light detection sensitivity is improved, overcome before existing flow cytometer to
Scatter the larger problem of light detection noise.
First optical filter 13 can be used for treating the scattering light that detection substance generates and be screened, so that required optical wavelength
It is transferred in forward detection module 1.Forward detection device 14 can detect the optical signal after screening.Microscope group 12 can be used for
The scattering light entered in forward detection module 1 is collected, and is transmitted in forward detection device 14 and is detected, is obtained to be checked
Survey the biophysical information of substance, such as size, refractive index.
As a kind of optional embodiment of the embodiment of the present invention, spectral module 52 includes: the second optical filter and has
The lens of positive light coke, the fluorescence signal in optical signal after the deflection that the second optical filter is used to export optical signal transmission module
Separation, the lens with positive light coke receive the fluorescence signal separated and focusing forms hot spot.Light in detecting module 53
Electric explorer receives the hot spot and is analyzed, and obtains biophysics, the biochemical information of substance to be detected, such as object to be detected
The complexity of matter internal structure, surface protein type, expression quantity etc..Specifically, the area of hot spot is less than photodetector
Photosensitive area.Photodetector can use avalanche diode.
As a kind of optional embodiment of the embodiment of the present invention, as shown in figure 4, optical signal transmission module 51 is also wrapped
Include: the both ends of optical fiber are arranged in optical fiber and collimation lens, collimation lens, it is therefore possible to use the optical fiber 54 with collimation lens.When
After reflecting mirror or prism in optical signal transmission module are deflected the optical signal that light collection module exports, it is quasi- to be transferred to band
In the optical fiber 54 of straight lens, optical fiber 54 is used for received optical signal transmission into spectral module 52.
Flow cytometer optical system provided in an embodiment of the present invention, by the way that light is arranged in the fluorescence detection module of system
Signal transmission module, the optical signal for exporting to light collection module deflect, and the setting of optical signal transmission module is received in light
Collect mutually disjoint any position within the scope of module full aperture, the optical signal dispersion that light collection module can be made to export, thus
It realizes the separation of optical signal caused by different exciting lights, and multiple photoelectric sensors and light-dividing device is set.Compared to existing skill
It directlys adopt multiple optical fiber in art to collect multi beam optical signal and be transmitted to the scheme detected in sensor, the present invention is real
The flow cytometer optical system of example offer, the optical signal exported by the optical signal transmission module of setting to light collection module are provided
Deflected, can make deflection after optical signal be split module reception, be not in directly adopt optical fiber collect when, due to
The limitation of fiber end face leads to the variation lines of measurement result so that optical fiber occurs minute movement optical signal and can not be collected by optical fiber
The problem of number increases, therefore, flow cytometer optical system provided in an embodiment of the present invention, even if there is the small range drift of optical path
It moves, the problem of optical signal can not be collected will not occur, so that the tolerable error of system increases, overcome and transmitted using optical fiber
The problem of Shi Yinguang collection module minute movement causes measurement result to deteriorate or fail, enhance the stability of system.In addition,
The diaphragm being arranged in the flow cytometer optical system can stop the optical signal of predetermined angle, will scatter without substance to be detected
Optical excitation signal separate, improve scattering light detection sensitivity, overcome existing flow cytometer forward scattering light detection to make an uproar
The larger problem of sound.
Although being described in conjunction with the accompanying the embodiment of the present invention, those skilled in the art can not depart from the present invention
Spirit and scope in the case where make various modifications and variations, such modifications and variations are each fallen within by appended claims institute
Within the scope of restriction.
Claims (10)
1. a kind of flow cytometer optical system characterized by comprising flow chamber, light source module, light collection module, forward direction
Detection module and fluorescence detection module；
The channel passed through for substance to be detected is provided in the flow chamber, the channel is transmissive to optical signal；
At least two excitation light sources are set in the light source module, and the excitation light source forms the focusing light not being overlapped in the channel
Spot；
At least a piece of optical mirror slip is set in the smooth collection module, and the optical mirror slip is for collecting substance to be detected through exciting
The scattering light and/or fluorescence that light source irradiation generates, and exported after being assembled to the scattering light and/or fluorescence；
The forward detection module is used to receive the scattering light that substance to be detected is generated through excitation light source irradiation, and to the scattering
Light is detected, and the biophysical information of substance to be detected is obtained；
The fluorescence detection module includes optical signal transmission module, spectral module and detecting module, the optical signal transmission module
Mutually disjoint any position is set within the scope of the optical mirror slip full aperture, for so that the light of light collection module output is believed
It number deflects, the spectral module is used to export the optical signal after deflection according to specific wavelength, and the detecting module is used for
The optical signal of spectral module output is detected, biophysics, the biochemical information of substance to be detected are obtained.
2. flow cytometer optical system according to claim 1, which is characterized in that the optical signal transmission module packet
It includes: any one in reflecting mirror, reflecting prism or refracting prisms.
3. flow cytometer optical system according to claim 1, which is characterized in that the forward detection module includes:
Diaphragm, the diaphragm are used to stop to be transferred to the optical signal of predetermined angle in the forward detection module.
4. flow cytometer optical system according to claim 3, which is characterized in that the diaphragm includes: aperture and resistance
Blend stop, the barrier rib is arranged in the aperture, for stopping the optical signal of predetermined angle.
5. flow cytometer optical system according to claim 1, which is characterized in that the forward detection module includes:
First optical filter and forward detection device, first optical filter are used to treat the scattering light that detection substance generates and are screened, institute
Forward detection device is stated to detect the optical signal after screening.
6. flow cytometer optical system according to claim 1, which is characterized in that the spectral module includes: second
Optical filter, second optical filter is for the fluorescence signal separation in the optical signal after deflecting.
7. flow cytometer optical system according to claim 6, which is characterized in that the spectral module includes: to have
The lens of positive light coke, the detecting module include multiple photodetectors, and the lens receive isolated optical signal and focusing
Hot spot is formed, the hot spot is less than the photosensitive area of photodetector.
8. flow cytometer optical system according to claim 6, which is characterized in that the image-forming range of the optical mirror slip
More than or equal to the optical length of the optical mirror slip to photodetector.
9. flow cytometer optical system according to claim 8, which is characterized in that the image space aperture of the optical mirror slip
Angle is not more than 5 degree.
10. flow cytometer optical system according to claim 1, which is characterized in that the optical signal transmission module is also
It include: optical fiber and collimation lens, the both ends of the optical fiber are arranged in the collimation lens, and the optical fiber is used for collimated lens
Received optical signal transmission is into the spectral module.
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| CN201910459771.XA CN110146430A (en) | 2019-05-29 | 2019-05-29 | A kind of flow cytometer optical system |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111855544A (en) * | 2020-07-31 | 2020-10-30 | 洹仪科技(上海)有限公司 | Fluorescence imaging device and imaging method thereof |
| CN112147044A (en) * | 2020-09-07 | 2020-12-29 | 桂林电子科技大学 | Spectrum subdivision type optical fiber distributed detection device for flow cytometer |
| CN112229781A (en) * | 2020-09-07 | 2021-01-15 | 桂林电子科技大学 | Improved spectrum subdivision type optical fiber distributed detection device of flow cytometer |
| CN113624666A (en) * | 2021-09-07 | 2021-11-09 | 清华大学 | Stream type imaging system based on dot matrix laser scanning |
| CN113899677A (en) * | 2021-09-17 | 2022-01-07 | 桂林优利特医疗电子有限公司 | Reflective light splitting module and light splitting method for flow cytometer detection |
| CN114486691A (en) * | 2022-02-16 | 2022-05-13 | 上海纬冉科技有限公司 | Portable energy detection equipment and debugging device thereof |
| WO2022164672A1 (en) * | 2021-01-27 | 2022-08-04 | Becton, Dickinson And Company | Flow cytometers including fiber optic light collectors, and methods of use thereof |
| CN115015178A (en) * | 2022-08-05 | 2022-09-06 | 天津迈科隆生物科技有限公司 | Optical detection device and blood analyzer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1910439A (en) * | 2004-01-23 | 2007-02-07 | 贝克曼考尔特公司 | System and method for multiple laser triggering |
| CN102331397A (en) * | 2011-07-08 | 2012-01-25 | 无锡荣兴科技有限公司 | Photoelectric sensor for statistic analysis of blood cells |
| CN108489885A (en) * | 2018-04-24 | 2018-09-04 | 中翰盛泰生物技术股份有限公司 | A kind of optical system and device for streaming phosphor collection |
| CN208155810U (en) * | 2018-04-24 | 2018-11-27 | 中翰盛泰生物技术股份有限公司 | A kind of optical system and device for streaming phosphor collection |
| CN210136163U (en) * | 2019-05-29 | 2020-03-10 | 中国科学院苏州生物医学工程技术研究所 | Optical system of flow cytometer |
-
2019
- 2019-05-29 CN CN201910459771.XA patent/CN110146430A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1910439A (en) * | 2004-01-23 | 2007-02-07 | 贝克曼考尔特公司 | System and method for multiple laser triggering |
| CN102331397A (en) * | 2011-07-08 | 2012-01-25 | 无锡荣兴科技有限公司 | Photoelectric sensor for statistic analysis of blood cells |
| CN108489885A (en) * | 2018-04-24 | 2018-09-04 | 中翰盛泰生物技术股份有限公司 | A kind of optical system and device for streaming phosphor collection |
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