CN116724122A - 用于浓缩和鉴定血液中微生物的系统、方法和设备 - Google Patents

用于浓缩和鉴定血液中微生物的系统、方法和设备 Download PDF

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Publication number
CN116724122A
CN116724122A CN202180085109.8A CN202180085109A CN116724122A CN 116724122 A CN116724122 A CN 116724122A CN 202180085109 A CN202180085109 A CN 202180085109A CN 116724122 A CN116724122 A CN 116724122A
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CN
China
Prior art keywords
microorganisms
sample
blood
lysis buffer
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180085109.8A
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English (en)
Chinese (zh)
Inventor
E·M·奥特克劳瑟
M·E·肖布
E·P·里奇
A·C·哈奇
S·A·撒切尔
R·T·希尔
C·S·朗西克
M·S·威尔逊
J·D·沃尔什
K·M·瑞丽
C·L·罗普
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Biofire Defense LLC
Original Assignee
Commissariat a lEnergie Atomique CEA
Biofire Diagnostics Inc
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Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Biofire Diagnostics Inc filed Critical Commissariat a lEnergie Atomique CEA
Publication of CN116724122A publication Critical patent/CN116724122A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202180085109.8A 2020-12-16 2021-12-14 用于浓缩和鉴定血液中微生物的系统、方法和设备 Pending CN116724122A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063126041P 2020-12-16 2020-12-16
US63/126,041 2020-12-16
PCT/US2021/063288 WO2022132754A1 (en) 2020-12-16 2021-12-14 Systems, methods, and apparatuses for concentration and identification of a microorganism from blood

Publications (1)

Publication Number Publication Date
CN116724122A true CN116724122A (zh) 2023-09-08

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ID=82058145

Family Applications (1)

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CN202180085109.8A Pending CN116724122A (zh) 2020-12-16 2021-12-14 用于浓缩和鉴定血液中微生物的系统、方法和设备

Country Status (7)

Country Link
US (1) US20240043941A1 (https=)
EP (1) EP4263795A4 (https=)
JP (1) JP2024501497A (https=)
CN (1) CN116724122A (https=)
AU (1) AU2021400825A1 (https=)
CA (1) CA3205110A1 (https=)
WO (1) WO2022132754A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117883846A (zh) * 2024-02-22 2024-04-16 深圳市睿迈生物科技有限公司 血液杂质过滤微流控芯片

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115806971A (zh) * 2022-11-22 2023-03-17 北京金匙医学检验实验室有限公司 一种血液样本病原体微生物宏基因组的去宿主方法
WO2024129945A2 (en) * 2022-12-16 2024-06-20 Synthego Corporation Consumable for centrifugation of biological cells with a variable collection chamber for supernatant removal

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1641944B1 (en) * 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
US8183046B2 (en) * 2007-01-11 2012-05-22 The Board Of Trustees Of The University Of Illinois Temperature resistant pH buffers for use at low temperatures
CN102317789B (zh) * 2008-10-31 2014-08-27 生物梅里埃公司 利用质谱法分离、表征和/或鉴定微生物的方法
EP2333105A1 (en) * 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
KR20140023326A (ko) * 2011-06-06 2014-02-26 비오까르띠 에스아 이온성 계면활성제에 의한 세포의 선택적 용해
CN103813845B (zh) * 2011-07-22 2016-08-17 生物梅里埃有限公司 从培养物离析微生物的方法和试剂盒
ES2938752T3 (es) * 2015-04-20 2023-04-14 Qiagen Gmbh Método, solución de lisis y kit para la reducción selectiva de ácidos nucleicos animales en una muestra
GB201511129D0 (en) * 2015-06-24 2015-08-05 Linea Ab Q Method of determining antimicrobial susceptibility of a microorganism
JP6979409B2 (ja) * 2016-04-08 2021-12-15 スリーエム イノベイティブ プロパティズ カンパニー 細胞溶解及び核酸増幅のためのプロセス
KR102638609B1 (ko) * 2017-07-27 2024-02-19 바이오메리욱스, 인코포레이티드. 격리 튜브
CN112368561A (zh) * 2018-05-25 2021-02-12 克维拉公司 用于选择性裂解血细胞和分离微生物细胞的方法和组合物

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117883846A (zh) * 2024-02-22 2024-04-16 深圳市睿迈生物科技有限公司 血液杂质过滤微流控芯片

Also Published As

Publication number Publication date
AU2021400825A1 (en) 2023-07-06
EP4263795A1 (en) 2023-10-25
EP4263795A4 (en) 2024-11-20
WO2022132754A1 (en) 2022-06-23
JP2024501497A (ja) 2024-01-12
US20240043941A1 (en) 2024-02-08
CA3205110A1 (en) 2022-06-23

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