JP2024501497A - 血液からの微生物の濃縮及び同定のためのシステム、方法、及び装置 - Google Patents

血液からの微生物の濃縮及び同定のためのシステム、方法、及び装置 Download PDF

Info

Publication number
JP2024501497A
JP2024501497A JP2023536804A JP2023536804A JP2024501497A JP 2024501497 A JP2024501497 A JP 2024501497A JP 2023536804 A JP2023536804 A JP 2023536804A JP 2023536804 A JP2023536804 A JP 2023536804A JP 2024501497 A JP2024501497 A JP 2024501497A
Authority
JP
Japan
Prior art keywords
microorganisms
sample
buffer
blood
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023536804A
Other languages
English (en)
Japanese (ja)
Other versions
JP2024501497A5 (https=
Inventor
クラウザー， エリザベス メアリー オット
マルタ エリザベス シャウブ，
エドワード プレストン リッチ，
アンドリュー カーター ハッチ，
ステファニー アン サッチャー，
ライアン ティー． ヒル，
クリストファー エス． ロンシック，
マーク エス． ウィルソン，
ジョン ディー． ウォルシュ，
カーク エム． ライリー，
クラーク エル． ローパー，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux Inc
Original Assignee
Biomerieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Inc filed Critical Biomerieux Inc
Publication of JP2024501497A publication Critical patent/JP2024501497A/ja
Publication of JP2024501497A5 publication Critical patent/JP2024501497A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2023536804A 2020-12-16 2021-12-14 血液からの微生物の濃縮及び同定のためのシステム、方法、及び装置 Pending JP2024501497A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063126041P 2020-12-16 2020-12-16
US63/126,041 2020-12-16
PCT/US2021/063288 WO2022132754A1 (en) 2020-12-16 2021-12-14 Systems, methods, and apparatuses for concentration and identification of a microorganism from blood

Publications (2)

Publication Number Publication Date
JP2024501497A true JP2024501497A (ja) 2024-01-12
JP2024501497A5 JP2024501497A5 (https=) 2024-12-23

Family

ID=82058145

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023536804A Pending JP2024501497A (ja) 2020-12-16 2021-12-14 血液からの微生物の濃縮及び同定のためのシステム、方法、及び装置

Country Status (7)

Country Link
US (1) US20240043941A1 (https=)
EP (1) EP4263795A4 (https=)
JP (1) JP2024501497A (https=)
CN (1) CN116724122A (https=)
AU (1) AU2021400825A1 (https=)
CA (1) CA3205110A1 (https=)
WO (1) WO2022132754A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115806971A (zh) * 2022-11-22 2023-03-17 北京金匙医学检验实验室有限公司 一种血液样本病原体微生物宏基因组的去宿主方法
WO2024129945A2 (en) * 2022-12-16 2024-06-20 Synthego Corporation Consumable for centrifugation of biological cells with a variable collection chamber for supernatant removal
CN117883846A (zh) * 2024-02-22 2024-04-16 深圳市睿迈生物科技有限公司 血液杂质过滤微流控芯片

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012507711A (ja) * 2008-10-31 2012-03-29 バイオメリュー・インコーポレイテッド 質量分析を使用した微生物の分離、キャラクタリゼーションおよび／または同定方法
JP2013512685A (ja) * 2009-12-08 2013-04-18 バイオカルティス、ソシエテ、アノニム 細胞の選択的溶解
US20180223327A1 (en) * 2015-06-24 2018-08-09 Q-Linea Ab Method for determining antimicrobial susceptibility of a microorganism
JP2020529915A (ja) * 2017-07-27 2020-10-15 ビオメリュー・インコーポレイテッド 隔離チューブ

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1641944B1 (en) * 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
US8183046B2 (en) * 2007-01-11 2012-05-22 The Board Of Trustees Of The University Of Illinois Temperature resistant pH buffers for use at low temperatures
KR20140023326A (ko) * 2011-06-06 2014-02-26 비오까르띠 에스아 이온성 계면활성제에 의한 세포의 선택적 용해
CN103813845B (zh) * 2011-07-22 2016-08-17 生物梅里埃有限公司 从培养物离析微生物的方法和试剂盒
ES2938752T3 (es) * 2015-04-20 2023-04-14 Qiagen Gmbh Método, solución de lisis y kit para la reducción selectiva de ácidos nucleicos animales en una muestra
JP6979409B2 (ja) * 2016-04-08 2021-12-15 スリーエム イノベイティブ プロパティズ カンパニー 細胞溶解及び核酸増幅のためのプロセス
CN112368561A (zh) * 2018-05-25 2021-02-12 克维拉公司 用于选择性裂解血细胞和分离微生物细胞的方法和组合物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012507711A (ja) * 2008-10-31 2012-03-29 バイオメリュー・インコーポレイテッド 質量分析を使用した微生物の分離、キャラクタリゼーションおよび／または同定方法
JP2013512685A (ja) * 2009-12-08 2013-04-18 バイオカルティス、ソシエテ、アノニム 細胞の選択的溶解
US20180223327A1 (en) * 2015-06-24 2018-08-09 Q-Linea Ab Method for determining antimicrobial susceptibility of a microorganism
JP2020529915A (ja) * 2017-07-27 2020-10-15 ビオメリュー・インコーポレイテッド 隔離チューブ

Also Published As

Publication number Publication date
AU2021400825A1 (en) 2023-07-06
EP4263795A1 (en) 2023-10-25
CN116724122A (zh) 2023-09-08
EP4263795A4 (en) 2024-11-20
WO2022132754A1 (en) 2022-06-23
US20240043941A1 (en) 2024-02-08
CA3205110A1 (en) 2022-06-23

Similar Documents

Publication Publication Date Title
US12203125B2 (en) Device for determining antimicrobial susceptibility of a microorganism
JP2023085281A (ja) 扱いにくい試料タイプのための試料調製
US12253513B2 (en) Methods and compositions for the selective lysis of blood cells and separation of microbial cells
US8647858B2 (en) Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis
EP2925891B1 (en) Apparatus and method for extracting microbial cells
US20100291536A1 (en) Sample processing cassette, system, and method
JP2024501497A (ja) 血液からの微生物の濃縮及び同定のためのシステム、方法、及び装置
US11702647B2 (en) Method for pretreatment of microbial cells
US9707555B2 (en) Method for pretreatment of microbial samples
JP2025542198A (ja) 分析物を単離するための方法、システム、および組成物
JP2025525592A (ja) 細胞溶解及び核酸回収
HK1252952B (en) Sample preparation for difficult sample types

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241213

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241213

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20251126

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260113

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260413