CN116616180A - Automatic proliferation inoculation equipment and method for plant seedling tissue culture - Google Patents
Automatic proliferation inoculation equipment and method for plant seedling tissue culture Download PDFInfo
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- CN116616180A CN116616180A CN202310665979.3A CN202310665979A CN116616180A CN 116616180 A CN116616180 A CN 116616180A CN 202310665979 A CN202310665979 A CN 202310665979A CN 116616180 A CN116616180 A CN 116616180A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 34
- 241000196324 Embryophyta Species 0.000 title claims abstract description 33
- 230000035755 proliferation Effects 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 137
- 230000035764 nutrition Effects 0.000 claims abstract description 35
- 235000016709 nutrition Nutrition 0.000 claims abstract description 35
- 239000002699 waste material Substances 0.000 claims abstract description 26
- 238000007599 discharging Methods 0.000 claims description 25
- 210000000078 claw Anatomy 0.000 claims description 22
- 229920001817 Agar Polymers 0.000 claims description 19
- 239000008272 agar Substances 0.000 claims description 19
- 230000006698 induction Effects 0.000 claims description 4
- 238000004064 recycling Methods 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000009471 action Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Transplanting Machines (AREA)
Abstract
The invention discloses automatic proliferation inoculation equipment and method for plant seedling tissue culture, comprising a cabinet, a mother bottle conveying belt, a nutrition bottle conveying belt, a seedling separating mechanism, a cutting mechanism, an operation box and a control system, wherein the mother bottle conveying belt and the nutrition bottle conveying belt are arranged in parallel and staggered up and down, a needle hooking seedling taking mechanism for grabbing seedlings on a mother bottle onto the seedling separating mechanism and a Y-axis servo walking sliding table for driving the needle hooking seedling taking mechanism to slide in a Y-axis manner and an X-axis servo walking sliding table for driving the needle hooking seedling taking mechanism to slide in an X-axis manner are arranged on the cabinet, a rotary cutting mechanism for cutting waste seedlings is arranged in the middle of the seedling separating mechanism, a mother bottle automatic centering mechanism is arranged on the mother bottle conveying belt, and a nutrition bottle automatic centering mechanism is arranged on the nutrition bottle conveying belt. The invention can well realize the automatic production of proliferation inoculation which is operated by manpower in the current plant seedling tissue culture, reduce the labor cost, prevent people from contacting with an inoculation object, improve the success rate of plant seedling tissue culture and increase the economic benefit.
Description
Technical Field
The invention relates to plant seedling tissue culture equipment, in particular to automatic proliferation inoculation equipment and method for plant seedling tissue culture.
Background
The plant seedling tissue culture inoculation in the market is basically carried out manually, so that more operators are needed, the efficiency is low, the sanitary condition is not well controlled, bacteria are easy to breed after people contact with seedlings, the death rate of the plant after seedling separation is high, and the economic benefit is influenced.
Disclosure of Invention
In order to solve the defects and shortcomings in the prior art, the invention provides the automatic production which can well realize proliferation inoculation which is operated manually in the current plant seedling tissue culture, reduces the labor cost, prevents people from contacting with an inoculation object, improves the success rate of plant seedling tissue culture, and increases economic benefits and economic benefits; by adopting the needle hooking seedling taking mechanism, the automatic proliferation inoculation equipment and method for plant seedling tissue culture can be used for taking no consideration of the positions of seedlings in the bottles and the sizes of roots.
The invention provides the following technical scheme: the utility model provides an automatic proliferation inoculation equipment of plant seedling tissue culture, includes rack, installs female bottle conveyer belt, nutrition bottle conveyer belt, branch seedling mechanism, cutting mechanism, control box and control system on the rack, female bottle conveyer belt and nutrition bottle conveyer belt parallel arrangement and dislocation from top to bottom, cutting mechanism is located the top of nutrition bottle conveyer belt, branch seedling mechanism is located the top of cutting mechanism, still be equipped with on the rack and be used for snatching the seedling on the female bottle and get seedling mechanism and drive to collude needle and get seedling mechanism and carry out Y-axis gliding Y-axis servo walking slip table and drive and collude needle and get seedling mechanism and carry out X-axis gliding X-axis servo walking slip table, branch seedling mechanism's middle part is equipped with the rotatory excision mechanism of excision useless seedling, be equipped with female bottle automatic centering mechanism on the female bottle conveyer belt, be equipped with nutrition bottle automatic centering mechanism on the nutrition bottle conveyer belt.
The invention can well realize the automatic production of proliferation inoculation which is operated by manpower in the current plant seedling tissue culture, reduce the labor cost, prevent people from contacting with an inoculation object, improve the success rate of plant seedling tissue culture and increase the economic benefit; the seedling hooking and picking mechanism is adopted, so that the seedling can be grasped once regardless of the position of the seedling in the bottle and the root size.
Preferably, the automatic centering mechanism of the mother bottle and the automatic centering mechanism of the nutrition bottle are respectively provided with a connecting rod automatic centering mechanism, and the connecting rod automatic centering mechanism comprises two positioning clamping claws, a connecting rod mechanism connected with the two positioning clamping claws and a positioning cylinder for driving the two positioning clamping claws to act.
This kind of structure ensures that female bottle automatic centering mechanism is to female bottle's positioning effect on the female bottle conveyer belt, and nutrition bottle automatic centering mechanism is to nutrition bottle's positioning effect on the nutrition bottle conveyer belt.
Preferably, the needle hooking and seedling taking mechanism comprises a needle hooking, a seedling taking cylinder for driving the needle hooking to act, a pneumatic vibrator for shaking agar on the needle hooking, a seedling discharging push plate for pushing seedlings on the needle hooking and a seedling discharging cylinder for driving the seedling discharging push plate to act.
The structure ensures the seedling taking effect and the seedling discharging effect of the needle hooking seedling taking mechanism.
Preferably, the seedling separating mechanism comprises a seedling separating box body, an agar collecting box arranged at the feeding end of the seedling separating box body and a seedling separating motor arranged at the discharging end of the seedling separating box body, wherein the seedling separating box body is provided with a pull Miao Gan, a pull Miao Liantiao for driving the pull Miao Gan, a pull Miao Lianlun and a pull Miao Dianji for driving the pull Miao Lianlun to act, the seedling separating motor is provided with a seedling separating pusher dog, and the seedling separating box body is also provided with a seedling guide plate and a seedling stacking stop lever.
The seedling pulling mechanism can well realize the separation of seedlings which are intersected together automatically after seedling taking, adopts a chain seedling pulling structure, has simple structure, high transmission efficiency, easy adjustment and durability, and adopts a seedling separating pusher dog matched seedling pulling structure to enable seedling separation to be easy to control, and has high success rate.
Preferably, the rotary cutting mechanism comprises a rotary cutting blade and a rotary cutting motor for driving the rotary cutting blade to act, the rotary cutting blade is positioned on one side of the middle part of the seedling separating box body, the seedling guide plate is positioned between the agar collecting box and the rotary cutting blade, and the seedling stacking baffle rod is positioned between the rotary cutting blade and the seedling guide plate.
The structure ensures the guiding, ordering and cutting effects of the seedlings.
Preferably, one side of the seedling separating box body is provided with a waste seedling collecting box matched with the rotary cutting blade, a waste seedling blowing head matched with the waste seedling collecting box and a waste seedling outlet, a waste seedling baffle is arranged above the waste seedling collecting box, and the position of the waste seedling baffle corresponds to the position of the waste seedling outlet.
The structure ensures that the part of the upper part of the seedling, which is not needed, can be cut off and blown to the waste seedling collecting box for centralized treatment on time.
Preferably, one side of the seedling separating box body is provided with an adjusting screw for adjusting the spacing between seedling guide plates; the discharge end of the seedling separating box body is also provided with a groove-shaped photoelectric sensor matched with the seedling separating motor and an induction piece matched with the groove-shaped photoelectric sensor.
The seedling pulling device has the advantages that seedling pulling is stable and reliable due to the structure, seedlings are ordered by the seedling guide plates with adjustable intervals, and the seedling pulling device is simple in structure, and stable and efficient in ordering; meanwhile, the seedling separation is easier to control, and the success rate is higher.
Preferably, the cutting mechanism comprises a cutting funnel seat, a broach cylinder, a broach positioning plate and a circle of swing cutting blades which are uniformly distributed and are arranged on the cutting funnel seat, the cutting edge direction of the swing cutting blades is inwards arranged, a plurality of blade swinging grooves matched with the swing cutting blades are formed in the side face of the cutting funnel seat, the broach positioning plate is sleeved on the cutting funnel seat, the bottom of the broach cylinder is connected with the broach positioning plate, the bottom of each swing cutting blade is connected with the cutting funnel seat through a rotating shaft, the outer side of the top of each swing cutting blade is connected with the cutting funnel seat through a tension spring, the broach cylinder drives the broach positioning plate to slide up and down on the cutting funnel seat, and the broach positioning plate and the tension spring control the swing cutting blades to swing around a rotating shaft so as to achieve folding and unfolding of the circle of swing cutting blades.
The structure can well realize that the seedlings after seedling separation are cut in an equal part after seedling taking, the work can be completed by adopting cutting power and only needing one cylinder to be matched with a tension spring, the structure is reasonable and simple to control, and the required time is short; the sizes of the seedlings which are cut out are consistent, and according to the sizes of the roots of the seedlings which are cultured, arbitrary equal parts can be designed.
Preferably, a broach cylinder mounting plate is fixed on the upper part of the cutting funnel seat, the broach cylinder is vertically mounted on the broach cylinder mounting plate, a piston rod of the broach cylinder is arranged downwards, and the tension spring is mounted at the bottom of the outer ring of the broach cylinder mounting plate through a tension spring seat; the broach cylinder is fixed on the broach cylinder mounting plate through a cylinder seat, the broach cylinder is positioned between two adjacent tension springs, the bottom of a piston rod of the broach cylinder is connected with one side of the broach positioning plate through a floating joint, and a speed regulating valve is arranged at the top of the broach cylinder; the upper portion of cutting funnel seat still is fixed with the cutting mechanism mounting panel, the cutting mechanism mounting panel is located the top of broach cylinder mounting panel, the one end bottom of cutting mechanism mounting panel is equipped with the jacking cylinder.
The structure is convenient for the installation of the broach cylinder and the tension spring, and ensures the action stability and reliability of the tension spring; the installation stability of the broach cylinder is ensured, and meanwhile, the action stability and reliability of the broach cylinder are ensured; the whole cutting mechanism is convenient to install, and meanwhile, the cutting mechanism is convenient to act.
A proliferation inoculation method of automatic proliferation inoculation equipment for plant seedling tissue culture is characterized by comprising the following steps of: the method comprises the following steps:
1) The positioning cylinders of the automatic centering mechanism of the mother bottle and the automatic centering mechanism of the nutrition bottle retract, the positioning claws are opened, when the mother bottle and the nutrition bottle reach the middle position of the preset positioning claws on the conveyor belt, the conveyor belt stops, the cylinders extend, the positioning claws are closed, and the tissue culture bottle with the position stopped is automatically positioned to the center position of the claws;
2) The Y-axis servo walking sliding table drives the needle hooking and seedling taking mechanism to act, a seedling taking cylinder of the needle hooking and seedling taking mechanism extends downwards to a preset position, the needle hooking is pricked into the root of a seedling, the seedling taking cylinder is retracted, and when the X-axis servo walking sliding table moves above the agar collection box with the seedling, the pneumatic vibrator is started to shake the agar brought out into the agar collection box for recycling;
3) When the hook needle seedling taking mechanism moves to a seedling discharging position on the seedling separating mechanism again, a seedling discharging cylinder drives a seedling discharging push plate to extend downwards, seedlings are pushed down to the seedling discharging position of a preset seedling separating box body, a pull Miao Dianji rotates, seedlings are driven forward by a pull Miao Gan arranged on a chain, the seedlings are sorted through a bevel guide plate, stacked seedlings can be blocked by a seedling stacking blocking rod to be sorted at the rear, when the seedlings pass through a cutting blade, unnecessary upper leaf sections are cut off and blown to a waste seedling collecting box to be treated in a concentrated manner in time, and after the seedlings are cut off to the seedling separating position, a seedling separating motor rotates a seedling separating pusher dog to be matched with the pulling Miao Dianji, and one plant is allocated to the cutting mechanism;
4) After the first seedling cut by the seedling separating mechanism is rotated to the seedling separating position, a lifting cylinder of the cutting mechanism is lifted, a seedling separating motor of the seedling separating mechanism drives a seedling separating pusher dog to rotate, the seedling is pulled into the cutting mechanism, the seedling is supported by a cutting funnel and an opened swinging cutting blade, a broach cylinder pulls a broach positioning plate, the cutting blade positioned by a rotating shaft at one end is folded inwards together, one-time cutting is completed, the broach cylinder drives the positioning plate to move down, and a tension spring is reset to open the cutting blade to wait for the next cutting.
The invention can well realize the automatic production of proliferation inoculation which is operated by manpower in the current plant seedling tissue culture, reduce the labor cost, prevent people from contacting with an inoculation object, improve the success rate of plant seedling tissue culture and increase the economic benefit and economic income; the seedling hooking and picking mechanism is adopted, so that the seedling can be grasped once regardless of the position of the seedling in the bottle and the root size.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic front view of the present invention;
FIG. 3 is a schematic side view of the present invention;
FIG. 4 is a schematic top view of the present invention;
FIG. 5 is a schematic structural view of a seedling separating mechanism in the invention;
FIG. 6 is a schematic top view of a seedling separating mechanism according to the present invention;
FIG. 7 is a schematic view of a cutting mechanism according to the present invention;
FIG. 8 is a partial cross-sectional view of a cutting mechanism of the present invention;
FIG. 9 is a flow chart of a proliferation inoculation method according to the present invention;
in the figure: 1. the automatic seedling raising and cutting machine comprises a cabinet, 2 parts of a parent bottle conveying belt, 3 parts of a nutrition bottle conveying belt, 4 parts of a seedling separating mechanism, 5 parts of a cutting mechanism, 6 parts of an operation box, 7 parts of a needle picking mechanism, 8.Y parts of a servo walking sliding table, 9.X parts of a servo walking sliding table, 10 parts of a rotary cutting mechanism, 11 parts of a seedling separating box body, 12 parts of an agar collecting box, 13 parts of a seedling separating motor, 14 parts of a pull Miao Gan, 15 parts of a pull Miao Liantiao, 16 parts of a pull Miao Lianlun, 17 parts of a pull Miao Dianji parts of a seedling separating claw, 19 parts of a seedling guiding plate, 20 parts of a seedling stacking stop lever, 21 parts of a rotary cutting blade, 22 parts of a rotary cutting motor, 23 parts of a waste seedling collecting box, 24 parts of a waste seedling outlet, 25 parts of a waste Miao Dangban, 26 parts of an adjusting screw, 27 parts of a groove type photoelectric sensor, 28 parts of a sensing piece, 29 parts of a parent bottle automatic centering mechanism, 30 parts of a nutrition bottle automatic centering mechanism, 31 parts of a cutting funnel seat, 32 parts of a broaching cylinder, 33 parts of a broaching cylinder positioning plate, 34 parts of a swing cutting blade, 35 parts of a blade swinging groove, 36 parts of a rotating shaft, 37 parts of a tension spring seat, 38 parts of a floating cylinder, 39 parts of a pulling cylinder seat, 39 parts of a lifting cylinder seat, 40 parts of a lifting cylinder, a lifting valve mounting plate, and mounting plates.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1-8, an automatic proliferation inoculation device for plant seedling tissue culture comprises a cabinet 1, a mother bottle conveying belt 2, a nutrition bottle conveying belt 3, a seedling separating mechanism 4, a cutting mechanism 5, an operation box 6 and a control system, wherein the mother bottle conveying belt 2 and the nutrition bottle conveying belt 3 are arranged in parallel and staggered up and down, the cutting mechanism 5 is positioned above the nutrition bottle conveying belt 3, the seedling separating mechanism 4 is positioned above the cutting mechanism 5, a needle hooking seedling taking mechanism 7 for grabbing seedlings on a mother bottle onto the seedling separating mechanism 4 and a Y-axis servo walking sliding table 8 for driving the needle hooking seedling taking mechanism 7 to slide along a Y-axis are further arranged on the cabinet 1, an X-axis servo walking sliding table 9 for driving the needle hooking seedling taking mechanism 7 to slide along a X-axis are further arranged on the cabinet 1, a rotary cutting mechanism 10 for cutting waste seedlings is arranged in the middle of the seedling separating mechanism 4, a mother bottle automatic centering mechanism 29 is arranged on the mother bottle conveying belt 2, and an automatic nutrition bottle centering mechanism 30 is arranged on the nutrition bottle conveying belt 3. The automatic centering mechanism 29 for the mother bottle and the automatic centering mechanism 30 for the nutrition bottle are both connecting rod automatic centering mechanisms, and each connecting rod automatic centering mechanism comprises two positioning clamping claws, a connecting rod mechanism for connecting the two positioning clamping claws and a positioning cylinder for driving the two positioning clamping claws to act. The needle hooking and seedling taking mechanism 7 comprises a needle hooking, a seedling taking cylinder for driving the needle hooking to act, a pneumatic vibrator for shaking agar on the needle hooking, a seedling discharging push plate for pushing seedlings on the needle hooking and a seedling discharging cylinder for driving the seedling discharging push plate to act.
The seedling separating mechanism comprises a seedling separating box body 11, an agar collecting box 12 arranged at the feeding end of the seedling separating box body 11 and a seedling separating motor 13 arranged at the discharging end of the seedling separating box body 11, wherein a pull Miao Gan 14, a pull Miao Liantiao and a pull Miao Lianlun 16 which drive the pull Miao Gan 14 and a pull Miao Dianji which drive the pull Miao Lianlun to act are arranged on the seedling separating box body 11, a seedling separating pusher dog 18 is arranged on the seedling separating motor 13, and a seedling guide plate 19 and a seedling stacking stop lever 20 are also arranged on the seedling separating box body 11. The rotary cutting mechanism 10 comprises a rotary cutting blade 21 and a rotary cutting motor 22 for driving the rotary cutting blade 21 to act, the rotary cutting blade 21 is positioned on one side of the middle part of the seedling separating box 11, the seedling guide plate 19 is positioned between the agar collecting box 12 and the rotary cutting blade 21, and the seedling stacking baffle 20 is positioned between the rotary cutting blade 21 and the seedling guide plate 19. One side of the seedling separating box 11 is provided with a waste seedling collecting box 23 matched with the rotary cutting blade 21 and a waste seedling blowing head and a waste seedling outlet 24 matched with the waste seedling collecting box 23, a waste seedling baffle 25 is arranged above the waste seedling collecting box 23, and the position of the waste seedling baffle 25 corresponds to the position of the waste seedling outlet 24. One side of the seedling separating box 11 is provided with an adjusting screw 26 for adjusting the spacing between the seedling guide plates 19. The discharge end of the seedling separating box body 11 is also provided with a groove-shaped photoelectric sensor 27 matched with the seedling separating motor 13 and an induction piece 28 matched with the groove-shaped photoelectric sensor 27.
The cutting mechanism 5 comprises a cutting funnel seat 31, a broach cylinder 32, a broach positioning plate 33 and three swinging cutting blades 34 which are uniformly distributed and installed on the cutting funnel seat 31, the cutting edge direction of the swinging cutting blades 34 is arranged inwards, three blade swinging grooves 35 which are matched with the swinging cutting blades 34 are formed in the side face of the cutting funnel seat 31, the broach positioning plate 33 is sleeved on the cutting funnel seat 31, the bottom of the broach cylinder 32 is connected with the broach positioning plate 33, the bottom of each swinging cutting blade is connected with the cutting funnel seat 31 through a rotating shaft 36, the outer side of the top of each swinging cutting blade is connected with the cutting funnel seat 31 through a tension spring 37, the broach cylinder 32 drives the broach positioning plate 33 to slide up and down on the cutting funnel seat 31, and the broach positioning plate 33 and the tension spring 37 control the swinging cutting blades 34 to swing around the rotating shaft 36 so as to achieve folding and unfolding of one circle of swinging cutting blades. The upper portion of cutting funnel seat 31 is fixed with broach cylinder mounting panel 38, and broach cylinder 32 is vertical to be installed on broach cylinder mounting panel 38, and the piston rod of broach cylinder 32 sets up downwards, and extension spring 37 passes through the spring seat 39 to be installed in the outer lane bottom of broach cylinder mounting panel 38. The broach cylinder 32 is fixed on the broach cylinder mounting plate 38 through a cylinder block 40, and the broach cylinder 32 is located between two adjacent extension springs, and the piston rod bottom of the broach cylinder 32 is connected with one side of the broach locating plate 33 through a floating joint 41, and the top of the broach cylinder 32 is provided with a speed regulating valve 42. The upper portion of the cutting funnel seat 31 is also fixed with a cutting mechanism mounting plate 43, the cutting mechanism mounting plate 43 is located above the broach cylinder mounting plate 38, and a jacking cylinder 44 is arranged at the bottom of one end of the cutting mechanism mounting plate 43.
The proliferation inoculation method of the automatic proliferation inoculation equipment for plant seedling tissue culture comprises the following steps:
1) Mother bottles (6 seedlings) and nutrition bottles (multiplication culture medium) are sent from corresponding conveyor belts;
2) The positioning cylinders of the automatic centering mechanism of the mother bottle and the automatic centering mechanism of the nutrition bottle retract, the positioning claws are opened, when the mother bottle and the nutrition bottle reach the middle position of the preset positioning claws on the conveyor belt, the conveyor belt stops, the cylinders extend, the positioning claws are closed, and the tissue culture bottle with the position stopped is automatically positioned to the center position of the claws;
3) The Y-axis servo walking sliding table drives the needle hooking and seedling taking mechanism to act, a seedling taking cylinder of the needle hooking and seedling taking mechanism extends downwards to a preset position, the needle hooking is pricked into the root of a seedling, the seedling taking cylinder is retracted, and when the X-axis servo walking sliding table moves above the agar collection box with the seedling, the pneumatic vibrator is started to shake the agar brought out into the agar collection box for recycling;
4) When the hook needle seedling taking mechanism moves to a seedling discharging position on the seedling separating mechanism again, a seedling discharging cylinder drives a seedling discharging push plate to extend downwards, seedlings are pushed down to the seedling discharging position of a preset seedling separating box body, a pull Miao Dianji rotates, seedlings are driven forward by a pull Miao Gan arranged on a chain, the seedlings are sorted through a bevel guide plate, stacked seedlings can be blocked by a seedling stacking blocking rod to be sorted at the rear, when the seedlings pass through a cutting blade, unnecessary upper leaf sections are cut off and blown to a waste seedling collecting box to be treated in a concentrated manner in time, and after the seedlings are cut off to the seedling separating position, a seedling separating motor rotates a seedling separating pusher dog to be matched with the pulling Miao Dianji, and one plant is allocated to the cutting mechanism;
5) After the first seedling cut by the seedling separating mechanism is rotated to the seedling separating position, a lifting cylinder of the cutting mechanism is lifted, a seedling separating motor of the seedling separating mechanism drives a seedling separating pusher dog to rotate, the seedling is pulled into the cutting mechanism, the seedling is supported by a cutting funnel and an opened swinging cutting blade, a broach cylinder pulls a broach positioning plate, the cutting blade positioned by a rotating shaft at one end is folded inwards together, one-time cutting is completed, the broach cylinder drives the positioning plate to move down, and a tension spring is reset to open the cutting blade to wait for the next cutting.
According to the embodiment of the invention, according to 6 seedlings per bottle, the cutting mechanism is designed according to 3 equal parts, so that one mother bottle can be divided into 3 bottles, and the empty bottles can be recycled along with a conveying line to a recycling point. The breeding inoculation in the nutrition bottle enters a cultivation room along with a conveying line for cultivation after the breeding inoculation is completed. According to the size of the root of the cultured seedling, the automatic equipment of 1 mother bottle for multiplying and inoculating any equivalent child bottle can be designed according to the working principle of the invention.
The spacing adjustment of the seedling guide plates in the invention means that the spacing between one side of the seedling guide plates and the inner wall of one side of the seedling separating box body is adjusted, so that the seedling separating box is suitable for seedlings of different types and sizes.
The seedling pulling motor, the seedling separating motor, the rotary cutting blade, the rotary cutting motor, the groove-type photoelectric sensor, the induction piece, the broach cylinder, the rotating shaft, the tension spring, the floating joint, the speed regulating valve, the control system and the jacking cylinder are all conventional components purchased in the market, and the specific structure and the working principle are conventional means, so that details are not needed.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. An automatic proliferation inoculation device for plant seedling tissue culture, which is characterized in that: the automatic seedling separating machine comprises a machine cabinet, a mother bottle conveying belt, a nutrition bottle conveying belt, a seedling separating mechanism, a cutting mechanism, an operating box and a control system, wherein the mother bottle conveying belt and the nutrition bottle conveying belt are arranged in parallel and staggered up and down, the cutting mechanism is located above the nutrition bottle conveying belt, the seedling separating mechanism is located above the cutting mechanism, a needle hooking and seedling taking mechanism used for grabbing seedlings on a mother bottle on the seedling separating mechanism and an X-axis servo walking sliding table used for driving the needle hooking and seedling taking mechanism to conduct Y-axis sliding and an X-axis servo walking sliding table used for driving the needle hooking and seedling taking mechanism to conduct X-axis sliding are further arranged on the machine cabinet, a rotary cutting mechanism used for cutting waste seedlings is arranged in the middle of the seedling separating mechanism, a mother bottle automatic centering mechanism is arranged on the mother bottle conveying belt, and an automatic nutrition bottle centering mechanism is arranged on the nutrition bottle conveying belt.
2. An automated propagation inoculation device for plant seedling tissue culture according to claim 1, wherein: the automatic centering mechanism of the mother bottle and the automatic centering mechanism of the nutrition bottle are respectively provided with a connecting rod automatic centering mechanism, and the connecting rod automatic centering mechanism comprises two positioning clamping claws, a connecting rod mechanism for connecting the two positioning clamping claws and a positioning cylinder for driving the two positioning clamping claws to act.
3. An automated propagation inoculation device for plant seedling tissue culture according to claim 2, wherein: the seedling hooking and taking mechanism comprises a hooking needle, a seedling taking cylinder for driving the hooking needle to act, a pneumatic vibrator for shaking agar on the hooking needle, a seedling discharging push plate for pushing seedlings on the hooking needle and a seedling discharging cylinder for driving the seedling discharging push plate to act.
4. An automated propagation inoculation device for plant seedling tissue culture according to claim 3, wherein: the seedling separating mechanism comprises a seedling separating box body, an agar collecting box arranged at the feeding end of the seedling separating box body and a seedling separating motor arranged at the discharging end of the seedling separating box body, wherein the seedling separating box body is provided with a pull Miao Gan, a pull Miao Liantiao for driving the pull Miao Gan, a pull Miao Lianlun and a pull Miao Dianji for driving the pull Miao Lianlun to act, the seedling separating motor is provided with a seedling separating pusher dog, and the seedling separating box body is also provided with a seedling guide plate and a seedling stacking stop lever.
5. An automated propagation inoculation device for plant seedling tissue culture according to claim 4, wherein: the rotary cutting mechanism comprises a rotary cutting blade and a rotary cutting motor for driving the rotary cutting blade to act, the rotary cutting blade is positioned on one side of the middle part of the seedling separating box body, the seedling guide plate is positioned between the agar collecting box and the rotary cutting blade, and the seedling stacking baffle rod is positioned between the rotary cutting blade and the seedling guide plate.
6. An automated propagation inoculation device for plant seedling tissue culture according to claim 4, wherein: the seedling separating box comprises a seedling separating box body, wherein one side of the seedling separating box body is provided with a seedling collecting box matched with a rotary cutting blade, a seedling blowing head matched with the seedling collecting box and a seedling outlet, a seedling baffle is arranged above the seedling collecting box, and the position of the seedling baffle corresponds to the position of the seedling outlet.
7. An automated propagation inoculation device for plant seedling tissue culture according to claim 1, wherein: an adjusting screw for adjusting the spacing between seedling guide plates is arranged on one side of the seedling separating box body; the discharge end of the seedling separating box body is also provided with a groove-shaped photoelectric sensor matched with the seedling separating motor and an induction piece matched with the groove-shaped photoelectric sensor.
8. An automated propagation inoculation device for plant seedling tissue culture according to claim 4, wherein: the utility model provides a cutting mechanism, including cutting funnel seat, broach cylinder, broach locating plate and round evenly distributed's the swing cutting blade of installing on cutting funnel seat, the cutting edge direction of swing cutting blade sets up inwards, the side of cutting funnel seat is equipped with the blade swinging groove of a plurality of cooperation swing cutting blades, broach locating plate cover is on cutting funnel seat, the broach locating plate is connected to the bottom of broach cylinder, and the bottom of every swing cutting blade all is connected with cutting funnel seat through the pivot, and the top outside of every swing cutting blade all is connected with cutting funnel seat through the extension spring, broach cylinder drive broach locating plate slides from top to bottom on cutting funnel seat, broach locating plate and extension spring control swing cutting blade revolute the swing of axle realize the receipts of round swing cutting blade and draw in and open.
9. An automated propagation inoculation device for plant seedling tissue culture according to claim 8, wherein: the upper part of the cutting funnel seat is fixedly provided with a broach cylinder mounting plate, the broach cylinder is vertically mounted on the broach cylinder mounting plate, a piston rod of the broach cylinder is arranged downwards, and the tension spring is mounted at the bottom of the outer ring of the broach cylinder mounting plate through a tension spring seat; the broach cylinder is fixed on the broach cylinder mounting plate through a cylinder seat, the broach cylinder is positioned between two adjacent tension springs, the bottom of a piston rod of the broach cylinder is connected with one side of the broach positioning plate through a floating joint, and a speed regulating valve is arranged at the top of the broach cylinder; the upper portion of cutting funnel seat still is fixed with the cutting mechanism mounting panel, the cutting mechanism mounting panel is located the top of broach cylinder mounting panel, the one end bottom of cutting mechanism mounting panel is equipped with the jacking cylinder.
10. A proliferation inoculation method of an automated proliferation inoculation apparatus for plant seedling tissue culture according to claim 8, wherein: the method comprises the following steps:
1) The positioning cylinders of the automatic centering mechanism of the mother bottle and the automatic centering mechanism of the nutrition bottle retract, the positioning claws are opened, when the mother bottle and the nutrition bottle reach the middle position of the preset positioning claws on the conveyor belt, the conveyor belt stops, the cylinders extend, the positioning claws are closed, and the tissue culture bottle with the position stopped is automatically positioned to the center position of the claws;
2) The Y-axis servo walking sliding table drives the needle hooking and seedling taking mechanism to act, a seedling taking cylinder of the needle hooking and seedling taking mechanism extends downwards to a preset position, the needle hooking is pricked into the root of a seedling, the seedling taking cylinder is retracted, and when the X-axis servo walking sliding table moves above the agar collection box with the seedling, the pneumatic vibrator is started to shake the agar brought out into the agar collection box for recycling;
3) When the hook needle seedling taking mechanism moves to a seedling discharging position on the seedling separating mechanism again, a seedling discharging cylinder drives a seedling discharging push plate to extend downwards, seedlings are pushed down to the seedling discharging position of a preset seedling separating box body, a pull Miao Dianji rotates, seedlings are driven forward by a pull Miao Gan arranged on a chain, the seedlings are sorted through a bevel guide plate, stacked seedlings can be blocked by a seedling stacking blocking rod to be sorted at the rear, when the seedlings pass through a cutting blade, unnecessary upper leaf sections are cut off and blown to a waste seedling collecting box to be treated in a concentrated manner in time, and after the seedlings are cut off to the seedling separating position, a seedling separating motor rotates a seedling separating pusher dog to be matched with the pulling Miao Dianji, and one plant is allocated to the cutting mechanism;
4) After the first seedling cut by the seedling separating mechanism is rotated to the seedling separating position, a lifting cylinder of the cutting mechanism is lifted, a seedling separating motor of the seedling separating mechanism drives a seedling separating pusher dog to rotate, the seedling is pulled into the cutting mechanism, the seedling is supported by a cutting funnel and an opened swinging cutting blade, a broach cylinder pulls a broach positioning plate, the cutting blade positioned by a rotating shaft at one end is folded inwards together, one-time cutting is completed, the broach cylinder drives the positioning plate to move down, and a tension spring is reset to open the cutting blade to wait for the next cutting.
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