CN116509779A - Sweet wormwood oral antibacterial liquid - Google Patents
Sweet wormwood oral antibacterial liquid Download PDFInfo
- Publication number
- CN116509779A CN116509779A CN202310337406.8A CN202310337406A CN116509779A CN 116509779 A CN116509779 A CN 116509779A CN 202310337406 A CN202310337406 A CN 202310337406A CN 116509779 A CN116509779 A CN 116509779A
- Authority
- CN
- China
- Prior art keywords
- antibacterial
- parts
- sweet wormwood
- oral
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 64
- 239000007788 liquid Substances 0.000 title claims abstract description 50
- 235000001405 Artemisia annua Nutrition 0.000 title claims abstract description 40
- 240000000011 Artemisia annua Species 0.000 title claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- 235000005979 Citrus limon Nutrition 0.000 claims abstract description 18
- 244000131522 Citrus pyriformis Species 0.000 claims abstract description 18
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 16
- 240000000691 Houttuynia cordata Species 0.000 claims abstract description 16
- 241000220317 Rosa Species 0.000 claims abstract description 16
- 235000006886 Zingiber officinale Nutrition 0.000 claims abstract description 16
- 235000008397 ginger Nutrition 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 235000013719 Houttuynia cordata Nutrition 0.000 claims abstract description 12
- 244000273928 Zingiber officinale Species 0.000 claims abstract 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 29
- 239000000243 solution Substances 0.000 claims description 21
- 239000011550 stock solution Substances 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 17
- 241000218628 Ginkgo Species 0.000 claims description 17
- 235000011201 Ginkgo Nutrition 0.000 claims description 17
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 17
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims description 14
- 240000006891 Artemisia vulgaris Species 0.000 claims description 14
- 244000242564 Osmanthus fragrans Species 0.000 claims description 14
- 235000019083 Osmanthus fragrans Nutrition 0.000 claims description 14
- 241000245665 Taraxacum Species 0.000 claims description 14
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims description 14
- 239000001525 mentha piperita l. herb oil Substances 0.000 claims description 14
- 235000019477 peppermint oil Nutrition 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 235000019202 steviosides Nutrition 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims description 7
- 230000003385 bacteriostatic effect Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 229940013618 stevioside Drugs 0.000 claims description 7
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims description 7
- 241000628997 Flos Species 0.000 claims description 6
- 230000032683 aging Effects 0.000 claims description 6
- 239000000686 essence Substances 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims description 5
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims description 5
- 235000012141 vanillin Nutrition 0.000 claims description 5
- 235000013717 Houttuynia Nutrition 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims description 3
- 239000004382 Amylase Substances 0.000 claims description 2
- 102000013142 Amylases Human genes 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 claims description 2
- 108090000145 Bacillolysin Proteins 0.000 claims description 2
- 108010059892 Cellulase Proteins 0.000 claims description 2
- 239000004367 Lipase Substances 0.000 claims description 2
- 102000004882 Lipase Human genes 0.000 claims description 2
- 108090001060 Lipase Proteins 0.000 claims description 2
- 102000035092 Neutral proteases Human genes 0.000 claims description 2
- 108091005507 Neutral proteases Proteins 0.000 claims description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 2
- 240000006365 Vitis vinifera Species 0.000 claims description 2
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 2
- 235000019418 amylase Nutrition 0.000 claims description 2
- 229940106157 cellulase Drugs 0.000 claims description 2
- 235000019421 lipase Nutrition 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000003002 pH adjusting agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 239000007921 spray Substances 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 230000005284 excitation Effects 0.000 abstract description 2
- 239000011664 nicotinic acid Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 21
- 238000002360 preparation method Methods 0.000 description 14
- 241000234314 Zingiber Species 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 6
- 230000007794 irritation Effects 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 235000010633 broth Nutrition 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 210000004087 cornea Anatomy 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 210000000214 mouth Anatomy 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000222122 Candida albicans Species 0.000 description 2
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 2
- 206010010726 Conjunctival oedema Diseases 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 206010015946 Eye irritation Diseases 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 241000283977 Oryctolagus Species 0.000 description 2
- 201000007100 Pharyngitis Diseases 0.000 description 2
- 241000605862 Porphyromonas gingivalis Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 230000005856 abnormality Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 210000000795 conjunctiva Anatomy 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 231100000013 eye irritation Toxicity 0.000 description 2
- 210000000554 iris Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002324 mouth wash Substances 0.000 description 2
- 229940051866 mouthwash Drugs 0.000 description 2
- 231100000286 mucous membrane, eye irritation or corrosion testing Toxicity 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000008354 sodium chloride injection Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 206010006326 Breath odour Diseases 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 208000025157 Oral disease Diseases 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 206010052891 Skin bacterial infection Diseases 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 239000004383 Steviol glycoside Substances 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 208000030194 mouth disease Diseases 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- IAIWVQXQOWNYOU-FPYGCLRLSA-N nitrofural Chemical compound NC(=O)N\N=C\C1=CC=C([N+]([O-])=O)O1 IAIWVQXQOWNYOU-FPYGCLRLSA-N 0.000 description 1
- 229960001907 nitrofurazone Drugs 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 229940041678 oral spray Drugs 0.000 description 1
- 239000000668 oral spray Substances 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 229930182488 steviol glycoside Natural products 0.000 description 1
- 235000019411 steviol glycoside Nutrition 0.000 description 1
- 150000008144 steviol glycosides Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/046—Aerosols; Foams
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/24—Thermal properties
- A61K2800/244—Endothermic; Cooling; Cooling sensation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Emergency Medicine (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Dispersion Chemistry (AREA)
Abstract
The invention provides an sweet wormwood oral antibacterial liquid. The antibacterial liquid disclosed by the invention is prepared by taking sweet wormwood, ginger, lemon, roxburgh rose, houttuynia cordata, honeysuckle and the like as main raw materials to perform bionic microecological fermentation, and active ingredients in the fermented raw materials are effectively extracted after decoction, so that the fermented raw liquid has good effects. The sweet wormwood oral antibacterial liquid has good taste, softness, no excitation and no peculiar smell, has specific fermentation smell and strong antibacterial effect, and is suitable for being used as an oral care liquid, in particular a spray type care liquid.
Description
Technical Field
The invention relates to the technical field of oral care, in particular to an oral antibacterial liquid.
Background
The oral cavity is located at the forefront of the digestive tract, one of the main ways for pathogenic microorganisms to invade, and is closely related to the healthy survival of humans. Bacterial and viral infections, air pollution, excessive use of sound, smoking and drinking, spicy dryness-heat stimulation of food and other factors can easily cause oral problems and throat inflammation, and if the oral problems and throat inflammation are not treated, mistreated and mistreated, periodontitis, chronic pharyngolaryngitis and the like can easily occur. The oral care can prevent oral diseases, reduce complications such as secondary infection and the like, promote appetite and comfort of patients, and is an important part in daily care work of the patients.
The conventional oral care solutions in the past have furacilin, hydrogen peroxide, sodium bicarbonate, physiological saline and the like, have no obvious effect, and the long-term application of the mouthwash containing antibacterial chemical components can cause the imbalance of oral bacteria, easily generate drug resistance and have relatively more side effects. The oral care solution prepared from various plant extracts is widely applied, but most of the obtained oral care solution has the problems of poor antibacterial effect, strong irritation and the like, so that more oral antibacterial solutions with strong antibacterial effect and small irritation need to be developed.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an sweet wormwood oral antibacterial liquid which has strong antibacterial effect on a plurality of bacteria such as staphylococcus aureus, escherichia coli, candida albicans, porphyromonas gingivalis and the like, and is nontoxic and non-irritating.
The sweet wormwood oral antibacterial liquid comprises the following components:
20 to 40 portions of antibacterial stock solution
Ethanol 5-8 parts
Peppermint oil 0.03-0.05 parts
0.3 to 1 part of vanillin essence
Stevioside 0-1 part
30 to 65 portions of water
The antibacterial stock solution is prepared from herba Artemisiae Annuae, folium Artemisiae Argyi, rhizoma Zingiberis recens, folium Ginkgo, flos Osmanthi Fragrantis, fructus Citri Limoniae, fructus Rosae Normalis, flos Lonicerae, herba Houttuyniae, and herba Taraxaci by performing enzymolysis, fermenting, and decocting.
The invention also provides a preparation method of the sweet wormwood oral antibacterial liquid.
Advantageous effects
The invention provides an sweet wormwood oral antibacterial liquid. The oral antibacterial liquid has strong antibacterial effect, mainly aims at removing bad breath and inhibiting oral harmful bacteria, and has certain inhibiting effect on some skin bacterial infections and some fungal infections. The antibacterial liquid disclosed by the invention is prepared by taking sweet wormwood, ginger, lemon, roxburgh rose, houttuynia cordata, honeysuckle and the like as main raw materials to perform bionic microecological fermentation, and effectively extracting active ingredients in the fermented raw materials after boiling, wherein the fermented raw liquid contains 3-5% of soluble solids, 3-4% of pH and 20-30 g/kg of total acid, so that the antibacterial liquid has good effects. The peppermint oil added in the invention has cool feel after acting on the oral cavity, and the ethanol is added to solve the problem of oil-water separation and solubility, and the spray form is better, so that the taste of the antibacterial liquid can be further improved by adding stevioside. In addition, the antibacterial liquid provided by the invention has the advantages of small irritation and low toxicity. In a word, the sweet wormwood oral antibacterial liquid has good taste, softness, no excitation and no peculiar smell, has specific fermentation smell and strong antibacterial effect, and is suitable for being used as an oral care liquid, in particular a spray type care liquid.
Detailed Description
The examples are presented for better illustration of the invention, but the invention is not limited to the examples. Those skilled in the art will appreciate that various modifications and adaptations of the embodiments described above are possible in light of the above teachings and are intended to be within the scope of the invention.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. Unless the context clearly differs, singular forms of expression include plural forms of expression. As used herein, it is understood that terms such as "comprising," "having," "including," and the like are intended to indicate the presence of a feature, number, operation, component, part, element, material, or combination. The terms of the present invention are disclosed in the specification and are not intended to exclude the possibility that one or more other features, numbers, operations, components, elements, materials or combinations thereof may be present or added. As used herein, "/" may be interpreted as "and" or "as appropriate.
In the present invention, the "parts" refer to parts by weight unless otherwise specified.
The invention provides an sweet wormwood oral antibacterial liquid which comprises the following components:
20 to 40 portions of antibacterial stock solution
Ethanol 5-8 parts
Peppermint oil 0.03-0.05 parts
Essence 0.3-1 parts
Stevioside 0-1 part
30 to 65 portions of water
The antibacterial stock solution is prepared from herba Artemisiae Annuae, folium Artemisiae Argyi, rhizoma Zingiberis recens, folium Ginkgo, flos Osmanthi Fragrantis, fructus Citri Limoniae, fructus Rosae Normalis, flos Lonicerae, herba Houttuyniae, and herba Taraxaci by performing enzymolysis, fermenting, and decocting.
In one embodiment, the enzyme used for enzymolysis is a complex enzyme of cellulase, lipase, neutral protease and amylase, and the weight ratio is 1-2: 1-2: 1-2: 1 to 2.
In one embodiment, the bacteria used for fermentation are composite bacteria of lactobacillus rhamnosus, bacillus subtilis and lactobacillus, and the weight ratio is 1-2: 1-2: 1 to 2.
In one embodiment, the weight ratio of sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus flower, lemon, roxburgh rose, honeysuckle, cordate houttuynia and dandelion is 2-8: 0 to 2: 6-10: 1 to 3:1 to 3: 8-12: 8-12: 2 to 5:2 to 5:1 to 3.
Preferably, the weight ratio of sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, cordate houttuynia and dandelion is 2-4: 0.1 to 0.5: 6-8: 1-2: 1-2: 8-10: 8-10: 2-3: 2-3: 1 to 2.
In one embodiment, the bacteriostatic stock solution is prepared by the following method:
crushing sweet wormwood, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata, dandelion and optional mugwort leaf to 12-65 meshes respectively, sieving, weighing according to a proportion, mixing uniformly, and adding warm water to soften for 1-24 hours; then adding complex enzyme into the mixture, adding water until the weight of the water is 5 to 15 times of the total weight of the raw materials, uniformly stirring, and carrying out enzymolysis for 5 to 10 days at 37+/-1 ℃ to obtain enzymolysis liquid; adding complex bacteria into the enzymolysis liquid, fermenting at 37+/-1 ℃ for 1-3 months, separating fermentation substrate by filter pressing equipment, filtering and collecting filtrate, and transferring the filtrate to a curing and boiling tank for curing and ageing to obtain fermentation stock solution.
Preferably, the amount of the complex enzyme is 10 to 20%, preferably 13 to 18% of the total amount of the raw materials.
Preferably, the amount of the composite bacteria is 10 to 20%, preferably 13 to 18% of the total amount of the raw materials.
Preferably, the water is used in an amount of 6 to 10 times the total amount of the raw materials.
Preferably, the decocting time is more than half a year.
In one embodiment, the bacteriostatic stock solution is 30-40 parts.
In one embodiment, the ethanol is 5 to 6 parts. According to the invention, the added ethanol not only can solve the problem of solubility of oil-water separation, but also can ensure that the antibacterial liquid has better spraying form when in spraying use.
In one embodiment, the peppermint oil is 0.03 to 0.04 parts. In the invention, the added peppermint oil can generate cool feel after acting on the oral cavity, and the using taste is improved.
In one embodiment, the flavour is selected from at least one of vanillin flavour, grape flavour, lemon flavour, preferably vanillin flavour. Preferably, it is 0.5 to 0.8 parts.
In one embodiment, the steviol glycoside is 0.01 to 0.3 parts. The stevioside is introduced into the sweet wormwood oral antibacterial liquid provided by the invention, so that the taste of the antibacterial liquid can be further improved.
In one embodiment, the water is 50 to 65 parts.
In one embodiment, the sweet wormwood oral bacteria inhibiting liquid further comprises a pH regulator, which is at least one selected from sodium hydroxide and sodium carbonate. The pH of the sweet wormwood oral antibacterial liquid is adjusted to 5.5-8.0, preferably 6.0-7.0 by adding a pH regulator.
In one embodiment, the sweet wormwood oral antibacterial liquid is mouthwash or oral spray.
The invention also provides a preparation method of the sweet wormwood oral antibacterial liquid, which comprises the following steps:
weighing antibacterial stock solution, ethanol, peppermint oil, essence and optional stevioside according to a proportion, dissolving the peppermint oil in the ethanol, uniformly mixing the antibacterial stock solution, the essence and the optional stevioside with water at 60-70 ℃, adding a pH regulator to adjust the pH to 5.5-8, transferring to a sterilizing tank, heating to 70-95 ℃ for sterilization, transferring to a mixing tank, cooling to 40-50 ℃, adding a solution of the peppermint oil in the ethanol, and uniformly stirring to obtain the sweet wormwood oral care solution.
In the present invention, the pH regulator may be added directly or may be added after being dissolved in water.
In one embodiment, a pH adjustor is added to adjust the pH to between 6.0 and 7.0.
In one embodiment, the method further comprises:
crushing sweet wormwood, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata, dandelion and optional mugwort leaf to 12-65 meshes respectively, sieving, weighing according to a proportion, mixing uniformly, and adding warm water to soften for 1-24 hours; then adding complex enzyme into the mixture, adding water until the weight of the water is 5 to 15 times of the total weight of the raw materials, uniformly stirring, and carrying out enzymolysis for 5 to 10 days at 37+/-1 ℃ to obtain enzymolysis liquid; adding complex bacteria into the enzymolysis liquid, fermenting at 37+/-1 ℃ for 1-3 months, separating fermentation substrate by filter pressing equipment, filtering and collecting filtrate, transferring the filtrate to a curing and boiling tank for curing and ageing for more than half a year to obtain fermentation stock solution.
The following description of embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is shown, however, only some, but not all embodiments of the invention are shown. All other embodiments, which can be made by a person skilled in the art without making any inventive effort, are intended to fall within the scope of the present invention.
The experimental methods in the following examples are conventional methods unless otherwise specified. The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications.
Preparation example: preparation of fermentation broths
Step 1) enzymolysis
Crushing and sieving sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata and dandelion respectively to 12-65 meshes, taking 24.75 parts of sweet wormwood, 3.50 parts of mugwort leaf, 60.00 parts of ginger, 10.50 parts of ginkgo leaf, 17.50 parts of sweet osmanthus, 85.00 parts of lemon, 87.50 parts of roxburgh rose, 27.50 parts of honeysuckle, 24.50 parts of houttuynia cordata and 10.00 parts of dandelion, mixing uniformly, adding 2000 parts of warm water, and softening for 20 hours; then 50.00 parts of complex enzyme (see Table 1) was added thereto, and water was added thereto until the total system weight was 3350 parts, and the mixture was stirred uniformly, and subjected to enzymolysis at 37.+ -. 1 ℃ for 7 days to obtain an enzymatic hydrolysate.
Step 2) fermentation
Adding 50.00 parts of complex bacteria (see table 1) into the enzymolysis liquid in the step 1), fermenting at 37+/-1 ℃ for 2 months, separating a fermentation substrate by filter pressing equipment, filtering and collecting filtrate, transferring the filtrate to a curing and boiling tank for curing and ageing, and boiling for more than half a year to obtain a fermentation stock solution.
Table 1: complex enzyme and complex bacterium composition
Comparative preparation 8: preparation of fermentation broths
Crushing and sieving sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata and dandelion respectively to 12-65 meshes, taking 24.75 parts of sweet wormwood, 3.50 parts of mugwort leaf, 60.00 parts of ginger, 10.50 parts of ginkgo leaf, 17.50 parts of sweet osmanthus, 85.00 parts of lemon, 87.50 parts of roxburgh rose, 27.50 parts of honeysuckle, 24.50 parts of houttuynia cordata and 10.00 parts of dandelion, mixing uniformly, adding 2000 parts of warm water, and softening for 20 hours; lactobacillus rhamnosus is then added thereto: bacillus subtilis: the weight ratio of the lactobacillus is 1:1:1, supplementing water to the total system weight of 3350 parts, uniformly stirring, fermenting for 3 months at 37+/-1 ℃, separating a fermentation substrate by filter pressing equipment, filtering and collecting filtrate, transferring the filtrate to a curing and boiling tank for curing and ageing for more than half a year, and obtaining fermentation stock solution. Wherein the content of soluble solids is 2.4%, and the total acid content is 13.6g/kg.
Comparative preparation 9: preparation of the extractive solution
Crushing and sieving sweet wormwood herb, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle flower, houttuynia cordata and dandelion respectively to 12-65 meshes, taking 24.75 parts of sweet wormwood herb, 3.50 parts of mugwort leaf, 60.00 parts of ginger, 10.50 parts of ginkgo leaf, 17.50 parts of sweet osmanthus flower, 85.00 parts of lemon, 87.50 parts of roxburgh rose, 27.50 parts of honeysuckle flower, 24.50 parts of houttuynia cordata and 10.00 parts of dandelion, uniformly mixing, boiling and extracting with 1000 parts by 3 of water, and merging the extracting solutions, wherein the content of soluble solid matters is 4.9%.
Examples 1-7 and comparative examples 1-9: preparation of sweet wormwood oral care solution
Filtering the fermentation stock solutions/extracting solutions of the preparation examples and the comparative preparation examples by diatomite, taking 350.75 parts, adding 5 parts of vanillin essence and 573.75 parts of water into the mixture, uniformly mixing the mixture at 60 ℃, then adding a solution of 3.35 parts of sodium hydroxide in 16.75 parts of water, adjusting the pH to 6.0-6.5, transferring to a sterilization tank, heating to 90 ℃ for sterilization for 1h, transferring to a mixing tank, cooling to 45 ℃, adding a solution of 0.4 part of peppermint oil pre-dissolved in 50.00 parts of ethanol, uniformly stirring to obtain the sweet wormwood oral care solution, and filling.
Test example 1: bacteriostasis test
1.1 preparation of bacteriostatic sheets sterilized and dried filter paper sheets with a diameter of 5mm were taken. 20 mu l of the sweet wormwood oral antibacterial liquid prepared in the example 1 is dripped into each slice, filter paper sheets are paved in a sterile plate, and the filter paper sheets are dried in a drying oven (37 ℃) in a semi-sealed state for standby.
1.2 preparation of negative control samples the same as 1.1, taking sterilized and dried filter paper sheets, adding 20 μl of sterile distilled water dropwise to each filter paper sheet, and drying for later use.
1.3 inoculation of a coating tool for test bacteria, flame sterilization with an alcohol burner and cooling, dipping at a concentration of 5X 10 5 ～5×10 6 cfu/ml test bacterial suspension is evenly coated on the surface of a nutrient agar culture medium flat plate. The plate was covered and dried at room temperature for 5min.
1.4 placing the bacteriostatic agent sample on the surface of a flat plate with sterile forceps. After the sticking, the sample wafer is lightly pressed by sterile forceps to be tightly attached to the surface of the flat plate. Each plate is pasted with 5 pieces of sample, wherein 4 pieces of antibacterial sheets and 1 piece of negative control sample are respectively pasted with 5 pieces of sample, the distance between the centers of the sample pieces is more than 25mm, the distance between the centers of the sample pieces and the periphery of the plate is more than 15mm, the plates are covered, and the plates are placed in a constant temperature incubator at 37 ℃ for culturing for 16-18 hours to observe results. Measuring the diameter of the inhibition ring (including the patch) by using a vernier caliper, recording, and taking the average value of the sizes of 4 inhibition rings on each plate as the reading of the plate; the test was performed in triplicate, and the average value was taken and recorded as the test result.
1.5 evaluating the antibacterial effect when the diameter of the specified antibacterial ring is larger than 7 mm. And if the diameter of the inhibition ring is smaller than or equal to 7mm, judging that the inhibition ring has no inhibition effect. And (3) judging that the test is qualified after the test is repeated for 3 times, wherein the test has a bacteriostatic effect result. The negative control group should have no inhibition ring. Otherwise the test is not effective.
The test results are shown in table 2:
table 2: antibacterial effect of oral care solution
Test results show that the sweet wormwood oral antibacterial liquid in the embodiments 1-7 has very excellent antibacterial activity, and has very strong inhibitory activity on various germs including staphylococcus aureus, escherichia coli, candida albicans and porphyromonas gingivalis; in comparative examples 1 to 8, the antibacterial activity of the antibacterial liquid is reduced more due to the insufficient glycolysis and fermentation process; the comparative example 9 adopts the extracting solution instead of the fermentation broth, and also has the problem of obviously insufficient antibacterial activity.
Test example 2: acute eye irritation test
With reference to the "rules for hygienic safety evaluation of sterile products", an anti (bacteriostatic) formulation for mucous membranes should be subjected to an ocular irritation test.
2.1 test animals were checked for abnormalities in both eyes of each New Zealand rabbit within 24 hours before preparation for the test, and were eliminated if abnormalities were found.
2.2 contamination method adopts self-contrast method of left and right sides of the same body, 0.1ml of test object stock solution is dripped into conjunctival sac of left eye, 3 eyes are passively closed for 4s in each group, and 30s later, the eyes are washed by sodium chloride injection. Sodium chloride injection was administered as a negative control by the right eye in an amount of 0.1 ml.
2.3 results record after dropping the test object, the eyes were passively closed for 4s,30s and then rinsed with physiological saline. Injury and recovery of conjunctiva, iris and cornea of New Zealand rabbit eyes were visually observed at 24 hours, 48 hours and 72 hours, respectively, and recorded. If the stimulus response is present, the condition can be continuously observed and recorded for 7 days, 14 days and 21 days; if no stimulus response occurs within 72 hours, or on day 7 or 14, the eye stimulus response is completely restored, the test can be terminated prematurely.
2.4 evaluation rules acute irritation responses of the cornea, iris and conjunctiva of rabbit eyes were scored according to the "disinfection technical Specification" (2002 edition) tables 2-13, and "average scores" were calculated for each animal at 3 different observation times (24, 48 and 72 h) for four aspects of cornea damage, iris damage, conjunctival congestion and conjunctival edema, respectively. The test subjects were evaluated for their eye irritation intensity according to the criteria of classification of eye irritation response in tables 2-14 and tables 2-15 of the "disinfection Specification" (2002 edition) by mean scoring and recovery time of corneal, iris and conjunctival congestion and edema, respectively, of the animal eyes. Each group was averaged.
The results are shown in Table 3:
table 3: acute eye irritation test results of oral Care solution
Test results show that the sweet wormwood oral antibacterial liquid provided by the embodiment of the invention has no stimulation to eyes and is high in safety; whereas comparative example 9, which uses an extract instead of a fermentation broth, had some degree of irritation to the eyes.
The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations to the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.
Claims (10)
1. An oral antibacterial solution of herba Artemisiae Annuae comprises:
20 to 40 portions of antibacterial stock solution
Ethanol 5-8 parts
Peppermint oil 0.03-0.05 parts
Essence 0.3-1 parts
Stevioside 0-1 part
30 to 65 portions of water
The antibacterial stock solution is prepared from herba Artemisiae Annuae, folium Artemisiae Argyi, rhizoma Zingiberis recens, folium Ginkgo, flos Osmanthi Fragrantis, fructus Citri Limoniae, fructus Rosae Normalis, flos Lonicerae, herba Houttuyniae, and herba Taraxaci by performing enzymolysis, fermenting, and decocting.
2. The antibacterial liquid according to claim 1, wherein the enzyme used for enzymolysis is a complex enzyme of cellulase, lipase, neutral protease and amylase, and the weight ratio is 1-2: 1-2: 1-2: 1 to 2; the bacteria used for fermentation are composite bacteria of lactobacillus rhamnosus, bacillus subtilis and lactobacillus, and the weight ratio is 1-2: 1-2: 1 to 2.
3. The antibacterial liquid according to claim 2 or 3, wherein the weight ratio of sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus flower, lemon, roxburgh rose, honeysuckle, cordate houttuynia and dandelion is 2-8: 0 to 2: 6-10: 1 to 3:1 to 3: 8-12: 8-12: 2 to 5:2 to 5:1 to 3.
4. A bacteriostatic liquid according to claim 3, characterized in that said bacteriostatic stock is prepared by the following method:
crushing sweet wormwood, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata, dandelion and optional mugwort leaf to 12-65 meshes respectively, sieving, weighing according to a proportion, mixing uniformly, and adding warm water to soften for 1-24 hours; then adding complex enzyme into the mixture, adding water until the weight of the water is 5 to 15 times of the total weight of the raw materials, uniformly stirring, and carrying out enzymolysis for 5 to 10 days at 37+/-1 ℃ to obtain enzymolysis liquid; adding complex bacteria into the enzymolysis liquid, fermenting at 37+/-1 ℃ for 1-3 months, separating fermentation substrate by filter pressing equipment, filtering and collecting filtrate, transferring the filtrate to a curing and boiling tank for curing and ageing for more than half a year to obtain antibacterial stock solution.
5. The antibacterial liquid according to claim 4, wherein the amount of the complex enzyme is 10-20%, preferably 13-18% of the total amount of the raw materials; the amount of the composite bacteria is 10 to 20% of the total amount of the raw materials, preferably 13 to 18%.
6. The antibacterial liquid according to claim 1, wherein the antibacterial stock solution is 30-40 parts; 5-6 parts of ethanol; 0.03-0.04 part of peppermint oil; the essence is at least one selected from vanillin essence, grape essence and lemon essence, and 0.5-0.8 part of the essence is added into the mixture; 50-65 parts of water.
7. The antibacterial liquid according to claim 3, wherein the weight ratio of sweet wormwood, mugwort leaf, ginger, ginkgo leaf, sweet osmanthus flower, lemon, roxburgh rose, honeysuckle, cordate houttuynia and dandelion is 2-4: 0.1 to 0.5: 6-8: 1-2: 1-2: 8-10: 8-10: 2-3: 2-3: 1 to 2.
8. The antibacterial solution according to any one of claims 1 to 7, wherein the sweet wormwood oral antibacterial solution further comprises a pH adjuster selected from at least one of sodium hydroxide and sodium carbonate.
9. The method for preparing the sweet wormwood oral antibacterial liquid according to claim 8, which comprises the following steps:
weighing antibacterial stock solution, ethanol, peppermint oil and essence according to a proportion, dissolving the peppermint oil in the ethanol, uniformly mixing the antibacterial stock solution, the essence and water at 60-70 ℃, adding a pH regulator to adjust the pH to 5.5-8, transferring to a sterilization tank, heating to 70-95 ℃ for sterilization, transferring to a mixing tank, cooling to 40-50 ℃, adding a solution of the peppermint oil in the ethanol, and uniformly stirring to obtain the sweet wormwood oral care solution.
10. The method of manufacturing according to claim 9, wherein the method further comprises:
crushing sweet wormwood, ginger, ginkgo leaf, sweet osmanthus, lemon, roxburgh rose, honeysuckle, houttuynia cordata, dandelion and optional mugwort leaf to 12-65 meshes respectively, sieving, weighing according to a proportion, mixing uniformly, and adding warm water to soften for 1-24 hours; then adding complex enzyme into the mixture, adding water until the weight of the water is 5 to 15 times of the total weight of the raw materials, uniformly stirring, and carrying out enzymolysis for 5 to 10 days at 37+/-1 ℃ to obtain enzymolysis liquid; adding complex bacteria into the enzymolysis liquid, fermenting at 37+/-1 ℃ for 1-3 months, separating fermentation substrate by filter pressing equipment, filtering and collecting filtrate, and transferring the filtrate to a curing and boiling tank for curing and ageing to obtain antibacterial stock solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310337406.8A CN116509779A (en) | 2023-03-31 | 2023-03-31 | Sweet wormwood oral antibacterial liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310337406.8A CN116509779A (en) | 2023-03-31 | 2023-03-31 | Sweet wormwood oral antibacterial liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116509779A true CN116509779A (en) | 2023-08-01 |
Family
ID=87394923
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310337406.8A Pending CN116509779A (en) | 2023-03-31 | 2023-03-31 | Sweet wormwood oral antibacterial liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116509779A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106174522A (en) * | 2016-07-28 | 2016-12-07 | 苏剑锋 | A kind of Herba Artemisiae Annuae Flos Lonicerae ferment and preparation method thereof |
| CN106344764A (en) * | 2016-08-31 | 2017-01-25 | 宋继武 | Traditional Chinese medicine oral cavity caring liquid and preparation method thereof |
| CN111388560A (en) * | 2020-04-16 | 2020-07-10 | 广东君道营养科技有限公司 | Traditional Chinese medicine composition for inhibiting formation of dental plaque, preparation method and application thereof |
-
2023
- 2023-03-31 CN CN202310337406.8A patent/CN116509779A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106174522A (en) * | 2016-07-28 | 2016-12-07 | 苏剑锋 | A kind of Herba Artemisiae Annuae Flos Lonicerae ferment and preparation method thereof |
| CN106344764A (en) * | 2016-08-31 | 2017-01-25 | 宋继武 | Traditional Chinese medicine oral cavity caring liquid and preparation method thereof |
| CN111388560A (en) * | 2020-04-16 | 2020-07-10 | 广东君道营养科技有限公司 | Traditional Chinese medicine composition for inhibiting formation of dental plaque, preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101796423B1 (en) | Composition and ladies genital area cleanser composition comprising composition for inducing Antiviral and antibacterial cause vaginitis and method for manufacturing the same | |
| CN111202822A (en) | Gynecological external antibacterial spray and preparation method thereof | |
| CN113081928A (en) | Plant bacteriostatic gel and preparation method and application thereof | |
| KR102148808B1 (en) | Feminine Cleanser Composition Comprising Natural Antimicrobial Agents | |
| CN101543658B (en) | Cervical cap for preventing and treating cervical erosion and preparation method thereof | |
| US20080075794A1 (en) | Natural antimicrobial and method of manufacturing the same | |
| CN108324922A (en) | A kind of gel combination of prevention and treatment gynaecological imflammation | |
| CN114796300A (en) | Preparation method of luffa whole juice freeze-dried powder, luffa whole juice freeze-dried powder and application thereof | |
| CN108042626B (en) | Composition with cerumen removing and bacteriostatic effects, preparation and application thereof | |
| KR20170061881A (en) | Natural Antiseptic Composition Containing Morus Alba Bark Extract | |
| CN108079165A (en) | A kind of gynaecology's washing lotion containing chitosan quaternary ammonium salt and preparation method thereof | |
| CN110251580B (en) | Composite antibacterial agent and preparation method and application thereof | |
| CN109602868A (en) | Plant essence liquid and preparation method thereof and sanitary napkin application | |
| CN116509779A (en) | Sweet wormwood oral antibacterial liquid | |
| CN114306526B (en) | Compound broadleaf holly leaf traditional Chinese medicine extract and application thereof in preparing shower gel or hand sanitizer | |
| CN109602813A (en) | A kind of bacteriostatic gel and preparation method thereof | |
| Bukhari et al. | In vitro biological evaluation and DNA damage protection activities of Cotoneaster afghanicus G. Klotz and Tamarix arceuthoides Bunge extracts | |
| CN113244140A (en) | Composition for regulating skin micro-ecological balance and application and preparation method thereof | |
| US9023405B2 (en) | Pharmaceutical composition using stryphnodendron extracts for treating HPV infections | |
| CN111012703A (en) | Pure natural pH balance care solution and preparation method thereof | |
| Handayany | Antibacterial Activity Test to Ethanol Extract of White Pumpkin Leaf (Lagenaria siceraria) Against Multiple Bacterial Pathogens | |
| CN114159362B (en) | Anti-dandruff oil-control hair composition and preparation method thereof | |
| CN113368193B (en) | Skin bacteriostatic gel and preparation method thereof | |
| CN109512743A (en) | A kind of natural bacteriostatic composition, man's bacteriostatic lotion and its preparation method and application | |
| WO2012000070A1 (en) | Pharmaceutical composition using stryphnodendron extracts for treating hpv infections |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |