CN116509775B - Rosewood seed skin care lotion and preparation method and application thereof - Google Patents
Rosewood seed skin care lotion and preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
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- NUHSROFQTUXZQQ-UHFFFAOYSA-N isopentenyl diphosphate Chemical compound CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
The invention provides rosewood seed skin care liquid and a preparation method and application thereof, and belongs to the technical field of skin care products. The preparation method comprises the following steps: under aseptic condition, adding culture medium into rosewood seed extract, inoculating schizophyllum commune seed solution and ganoderma lucidum mycelia seed solution, fermenting, adding carbon quantum dots, fermenting to logarithmic phase, adopting intermittent ultraviolet irradiation until fermentation is completed, and performing solid-liquid separation to obtain supernatant, namely rosewood seed skin care liquid. The rosewood seed skin lotion is mainly used for preparing skin care products, can realize the effects of resisting wrinkles, resisting acnes and whitening, can enable rosewood seeds to be more effectively utilized in cosmetic base materials, and improves the application value of rosewood seeds in the field of skin care products.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to rosewood seed skin care liquid and a preparation method and application thereof.
Background
Rosewood (Bixa orellana) is a shrub of the genus rosewood of the family redaceae, which is often referred to as a "annatto" or lipstick tree. Rosewood seed extract has been found to have a whitening effect in cosmetic applications, and also to act as a photoprotectant and moisturizer. The rosewood seed extract can limit the proliferation of sebaceous gland cells, block the signal of inducing hyperkeratosis, prevent hyperkeratosis of keratinocytes, and has a certain effect of reducing inflammation (Zhou Ying, ma Ping, cai Xinfen, shen Lingyan, huo Gang. The effects of controlling oil by the essence of lentil seed and rosewood seed extract and improving pores [ J ]. Spice flavor cosmetics [ 2022 (01): 64-67 ]).
The prior art has focused mainly on the research and development of compositions of rosewood seed extracts (CN 112972295A, CN108379180 a), and no fermentation research of rosewood seed extracts has been found.
Disclosure of Invention
The rosewood seed skin care liquid, the preparation method and the application thereof provided by the invention are used for preparing a ferment with the functions of resisting wrinkles, resisting acnes, whitening and the like by fermenting the rosewood seed extract.
The invention provides a preparation method of rosewood seed skin care lotion, which comprises the following steps:
under aseptic condition, adding culture medium into rosewood seed extract, inoculating schizophyllum commune seed solution and ganoderma lucidum mycelia seed solution, fermenting, adding carbon quantum dots, fermenting to logarithmic phase, adopting intermittent ultraviolet irradiation until fermentation is completed, and performing solid-liquid separation to obtain supernatant, namely rosewood seed skin care liquid.
Further, the volume ratio of the rosewood seed leaching solution, the culture medium, the schizophyllum commune seed solution and the ganoderma lucidum mycelia seed solution is 800-1200: 1800-2200: 300-500: 300-500.
Further, the fermentation temperature is 25-30 ℃; the fermentation time was 120h.
Further, the addition amount of the carbon quantum dots is 10 mg/L; the size of the carbon quantum dots was 1.8 nm.
Further, the intermittent ultraviolet irradiation is specifically: and (5) turning on an ultraviolet lamp in the fermentation tank every 0.5-2 hours to irradiate for 10-20 s until the fermentation is finished.
Further, the rosewood seed extract is prepared by the following steps:
1) Grinding and crushing rosewood seeds at low temperature, and screening to obtain rosewood seed powder;
2) Adding ethanol into rosewood seed powder for leaching, performing solid-liquid separation, and performing reduced pressure distillation on the obtained supernatant to obtain rosewood seed leaching solution.
Further, in the step 1), the temperature of low-temperature grinding and crushing is-20 to-1 ℃.
Further, in the step 2), the mass-volume ratio of the rosewood seed powder to the ethanol is 1:1-5; the leaching temperature is-10-1 ℃; the temperature of the reduced pressure distillation was 50 ℃.
The invention also provides the rosewood seed skin care lotion prepared by any one of the preparation methods.
The invention also provides application of any rosewood seed skin lotion in skin care products, wherein the skin care products have at least one of anti-wrinkle, whitening and acne removing effects.
The invention has the following advantages:
according to the invention, rosewood seeds are used as raw materials, schizophyllum commune and ganoderma lucidum mycelia are adopted for mixed fermentation, carbon quantum dots and intermittent ultraviolet irradiation are adopted in the fermentation process to promote the schizophyllum commune and ganoderma lucidum to generate antioxidant, anti-acne and whitening substances, and the obtained rosewood seed skin care liquid can realize the effects of resisting wrinkles, resisting acnes and whitening, so that the rosewood seeds are more effectively utilized in cosmetic base materials, and the application value of the rosewood seeds in the field of skin care products is improved.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.
An embodiment of the invention provides a preparation method of rosewood seed skin care lotion, which comprises the following steps:
under aseptic condition, adding culture medium into rosewood seed extract, inoculating schizophyllum commune seed solution and ganoderma lucidum mycelia seed solution, fermenting, adding carbon quantum dots, fermenting to logarithmic phase, adopting intermittent ultraviolet irradiation until fermentation is completed, and performing solid-liquid separation to obtain supernatant, namely rosewood seed skin care liquid.
According to the preparation method of the rosewood seed skin care liquid, two kinds of bacteria including schizophyllum commune and ganoderma lucidum mycelia are adopted for mixed fermentation, wherein the schizophyllum commune can secrete schizophyllum commune polysaccharide and other components with acne removing effect, and the ganoderma lucidum mycelia can secrete whitening factors including ganoderma triterpene, polypeptide, polysaccharide and other components. Meanwhile, in the fermentation process, under the intermittent ultraviolet irradiation, the ultraviolet light and ozone generated by the ultraviolet irradiation have strong oxidation effect, so that schizophyllum commune and ganoderma lucidum mycelia can be stimulated to further generate antioxidant functional components. In addition, in the fermentation process, the carbon quantum dots can also promote the generation of antioxidants, anti-acne and whitening substances in schizophyllum commune and ganoderma lucidum by adjusting the enzyme activity, improving the cell membrane permeability and the like, so that the obtained skin care liquid has the effects of resisting wrinkles, resisting acnes and whitening and protecting skin.
In one embodiment of the invention, the volume ratio of the rosewood seed extract, the culture medium, the schizophyllum commune seed liquid and the ganoderma lucidum mycelia seed liquid is 800-1200: 1800-2200: 300-500: 300-500. Preferably, the volume ratio of the rosewood seed leaching solution to the culture medium to the schizophyllum commune seed solution to the ganoderma lucidum mycelia seed solution is 1000:2000:350:350. compared with other seed solutions, the schizophyllan polysaccharide can be produced after the schizophyllan is fermented, and the ganoderma lucidum mycelium can produce the whitening factor ganoderma lucidum triterpene, so that the schizophyllan is more suitable for preparing skin care liquid.
In one embodiment of the invention, the culture medium is prepared by mixing the following raw materials: glucose 50 g/L, yeast extract 4g/L, potassium dihydrogen phosphate 0.5 g/L, magnesium sulfate heptahydrate 0.6 g/L, and vitamin B1 50 mg/L. It should be noted that the medium used in the present invention is a liquid medium.
In one embodiment of the invention, the fermentation temperature is 25-30 ℃. Preferably, the fermentation temperature is 28 ℃. Preferably, the fermentation time is 120 hours.
In one embodiment of the invention, the addition amount of the carbon quantum dots is 10 mg/L, and the size of the carbon quantum dots is 1.8 nm. The carbon quantum dots have various structures, and can enter the inside of plant cells and even reach cell nuclei; can also participate in the whole life activity period of plant growth; can also regulate the catalytic activity of various biological enzymes. Therefore, the invention comprehensively considers the properties of each raw material and the carbon quantum dot, adds 10 mg/L carbon quantum dot with the size of 1.8 nm, and promotes the generation of antioxidant, anti-acne and whitening substances in schizophyllum and ganoderma lucidum by regulating the enzyme activity, improving the cell membrane permeability and the like through the carbon quantum dot.
In an embodiment of the present invention, the intermittent ultraviolet irradiation specifically includes: and (5) turning on an ultraviolet lamp in the fermentation tank every 0.5-2 hours to irradiate for 10-20 s until the fermentation is finished. Ultraviolet rays generated by ultraviolet light UV irradiation can damage hyphae, the hyphae can correspondingly generate stress reaction, and antioxidant substances are secreted to resist damage, so that more antioxidant substances are generated in the fermentation process.
In one embodiment of the invention, after fermentation, the supernatant is obtained through solid-liquid separation, wherein the rotation speed during solid-liquid separation is 8500-15000 rpm.
In an embodiment of the invention, the rosewood seed leaching solution is prepared by the following steps:
1) Grinding and crushing rosewood seeds at low temperature, and screening to obtain rosewood seed powder;
2) Adding ethanol into rosewood seed powder for leaching, performing solid-liquid separation, and performing reduced pressure distillation on the obtained supernatant to obtain rosewood seed leaching solution.
The rosewood seed leaching solution provided by the embodiment of the invention is prepared by crushing rosewood seeds, screening, adding ethanol, and leaching under the condition of aseptic operation to obtain the rosewood seed leaching solution with the polar and nonpolar active ingredients.
In the step 1), the temperature of low-temperature grinding and crushing is-20 to-1 ℃. When sieving, the size of the sieve hole is 50-150 μm.
In the step 2), the mass-volume ratio of the rosewood seed powder to the ethanol is 1:1-5; it is noted that the unit of the mass-volume ratio is kg: l. Wherein the volume concentration of the ethanol is 75%. Specifically, in the step 2), the leaching temperature is-10-1 ℃. The leaching rotating speed is 100-500 pm. The leaching time is 60-130 hours. More specifically, in the step 2), the rotation speed during solid-liquid separation is 1500-10000 rpm. Preferably, in step 2), the temperature of the reduced pressure distillation is 50 ℃; ethanol may be removed by distillation under reduced pressure.
The embodiment of the invention also provides the rosewood seed skin care lotion prepared by any one of the preparation methods.
The embodiment of the invention also provides application of any one of the rosewood seed skin lotion in skin care products, wherein the skin care products have at least one of anti-wrinkle, whitening and acne removing effects. The rosewood seed skin care liquid is mainly used in the fields of skin care products and medical cosmetology, and the method realizes the generation of functional rosewood seed fermentation products and has great potential for the development of later medical and aesthetic products.
The invention will be described in detail with reference to examples.
Example 1A method for preparing rosewood seed skin care lotion comprises the following steps:
1) Weighing 1.5. 1.5 kg rosewood seeds, placing into a stainless steel grinding tank, and grinding and crushing at low temperature (-3 ℃) to obtain rosewood seed powder; sieving with a classifying sieving machine, wherein the mesh size is 100 μm, and collecting rosewood seed powder smaller than 100 μm;
2) Adding 2.35-L volume percent of 75 percent ethanol into 1-kg rosewood seed powder, putting into an extraction reaction kettle, and leaching for 72 hours at-1 ℃ and 150 rpm; after leaching, centrifuging at 10000rpm to perform solid-liquid separation; transferring the supernatant into a reduced pressure distiller, distilling at 50deg.C to remove ethanol to obtain rosewood seed extract;
3) Pouring the 1L rosewood seed leaching solution into a 5L aseptic fermentation tank under aseptic conditions, adding a pre-sterilized 2L culture medium, inoculating 350 mL schizophyllum strain seed solution and 350 mL ganoderma lucidum mycelia seed solution, fermenting at 28 ℃ until the logarithmic phase, adding carbon quantum dots, wherein the size of the carbon quantum dots is 1.8 nm, and the adding amount of the carbon quantum dots is 10 mg/L; fermenting to mid-logarithmic phase, adopting intermittent ultraviolet irradiation, and turning on an ultraviolet lamp in a fermentation tank for 10s every 1h until the fermentation is finished at 120h;
wherein the culture medium is prepared by mixing the following raw materials: glucose 50 g/L, yeast extract 4g/L, potassium dihydrogen phosphate 0.5 g/L, magnesium sulfate heptahydrate 0.6 g/L, and vitamin B1 50 mg/L;
4) After fermentation, performing solid-liquid separation at 10000rpm to obtain supernatant, namely rosewood seed skin care liquid.
Example 2A method for preparing rosewood seed skin care lotion comprises the following steps:
1) Weighing 1.5. 1.5 kg rosewood seeds, placing into a stainless steel grinding tank, and grinding and crushing at low temperature (-10 ℃) to obtain rosewood seed powder; sieving with a classifying sieving machine, wherein the mesh size is 50 μm, and collecting rosewood seed powder smaller than 50 μm;
2) Adding 1.35. 1.35L volume of 75% ethanol into 1. 1 kg rosewood seed powder, placing into an extraction reaction kettle, and leaching at-10deg.C and 250rpm for 72 hr; after the leaching is finished, carrying out solid-liquid separation by centrifugation at 8500 rpm; transferring the supernatant into a reduced pressure distiller, distilling at 50deg.C to remove ethanol to obtain rosewood seed extract;
3) Under the aseptic condition, pouring the 1L rosewood seed leaching solution into a 5L aseptic fermentation tank, adding a pre-sterilized 1.8L culture medium, and inoculating 300 mL schizophyllum strain seed solution and 300 mL ganoderma lucidum mycelia seed solution. The culture temperature is 28 ℃, the carbon quantum dots are added until the fermentation is carried out in the logarithmic phase, the size of the carbon quantum dots is 1.8 nm, and the addition amount of the carbon quantum dots is 10 mg/L; fermenting to mid-logarithmic phase, and irradiating with intermittent ultraviolet light, wherein every 0.5. 0.5 h of the fermentation tank is turned on for 10s until 120h of fermentation is completed;
wherein the culture medium is prepared by mixing the following raw materials: glucose 50 g/L, yeast extract 4g/L, potassium dihydrogen phosphate 0.5 g/L, magnesium sulfate heptahydrate 0.6 g/L, and vitamin B1 50 mg/L;
4) After fermentation, solid-liquid separation is carried out at 8500 rpm, and supernatant liquid, namely rosewood seed skin care liquid, is obtained.
Example 3A method for preparing rosewood seed skin care lotion comprises the following steps:
1) Weighing 1.5. 1.5 kg rosewood seeds, placing into a stainless steel grinding tank, and grinding and crushing at low temperature (-20 ℃) to obtain rosewood seed powder; sieving with a classifying sieving machine, wherein the mesh size is 150 μm, and collecting rosewood seed powder smaller than 150 μm;
2) Adding 5-L volume percent of 75 percent ethanol into 1-kg rosewood seed powder, putting the mixture into an extraction reaction kettle, and leaching the mixture for 128 hours at-1 ℃ and 500 rpm; after leaching, carrying out solid-liquid separation by centrifugation at 1500 rpm; transferring the supernatant into a reduced pressure distiller, distilling at 50deg.C to remove ethanol to obtain rosewood seed extract;
3) Under the aseptic condition, pouring the 1L rosewood seed leaching solution into a 7L aseptic fermentation tank, adding a pre-sterilized 3L culture medium, and inoculating 500 mL schizophyllum strain seed solution and 500 mL ganoderma lucidum mycelia seed solution. The culture temperature is 28 ℃, the carbon quantum dots are added until the fermentation is carried out in the logarithmic phase, the size of the carbon quantum dots is 1.8 nm, and the addition amount of the carbon quantum dots is 10 mg/L; fermenting to mid-logarithmic phase, adopting intermittent ultraviolet irradiation, and turning on an ultraviolet lamp in a fermentation tank for 10s every 1h until the fermentation is finished at 120h;
wherein the culture medium is prepared by mixing the following raw materials: glucose 50 g/L, yeast extract 4g/L, potassium dihydrogen phosphate 0.5 g/L, magnesium sulfate heptahydrate 0.6 g/L, and vitamin B1 50 mg/L;
4) After fermentation, carrying out solid-liquid separation at 15000rpm to obtain supernatant, namely rosewood seed skin care liquid.
Comparative example 1
The difference is that only rosewood seed extract is prepared, without step 3), step 4) as in example 1.
Comparative example 2
The difference from example 1 is that, when the rosewood seed extract is fermented, the fermentation is carried out to mid-log phase, and no intermittent ultraviolet irradiation is carried out.
Comparative example 3
The difference is that the seed solutions of schizophyllum commune and ganoderma lucidum are not inoculated during fermentation of the rosewood seed extract as in example 1.
Comparative example 4
The difference is that the oyster mushroom strain is inoculated when the rosewood seed extract is fermented as in example 1.
Comparative example 5
The difference is that the fermentation was carried out to the log phase before adding no carbon quantum dots as in example 1.
Test example 1Anti-wrinkle test
Subject screening work is referenced 2015 edition, "cosmetic safety Specification". 30 healthy male and female subjects are recruited, the age is 18-45 years, and the removal conditions of volunteers are in accordance with the inclusion removal standard of cosmetic contact dermatitis diagnosis standard and treatment principle.
After the subjects clean the face in the morning and evening every day, the products are used according to the instructions of the use of the products, 0.3g to 0.5g of the skin care liquid obtained in the examples and the comparative examples are used each time, the skin care liquid is gently massaged until the skin care liquid is absorbed, and other anti-wrinkle skin care products are stopped during the experiment. The test part is the left cheek part and the right cheek part of the face. During experimental test, the tested part is cleaned by clean water, and data acquisition is carried out after sitting still for 30min in a constant temperature and humidity environment with the temperature of 21.0+/-1.0 ℃ and the relative humidity of 50+/-10% RH, and a self-evaluation questionnaire is filled in. Dispensing the sample and instructions for use of the product. Subjects were used according to instructions for use, with a period of 28 days, facial dermatological anti-wrinkle efficacy tests were performed on day 0 and day 28, respectively, using a skin microscope and active skin surface analysis system VisioScan for analytical testing, and the controls were selected to follow themselves. The average value of the effect after 28 days of use is shown in table 1.
Table 1 average of face index after 28 days of use
Test example 2Whitening test
And (3) checking according to the specific requirements of a cosmetic freckle removing and whitening efficacy testing method of 2015 edition of cosmetic safety technical Specification. The total number of subjects is 100, wherein 50 men and 50 women are aged 22-59 years, and the average age (40.5+/-5.2) is in accordance with the volunteer selection standard and the exclusion standard of the subjects.
And (3) establishing an ultraviolet blackening model: the minimum erythema dose (minimal erythema dose, MED) of the backs of the eligible subjects in the group was first tested and then 3 areas no less than 6 cm were selected at the test site 2 The test product region and the negative control region are respectively arranged, the interval between each test region is not less than 1.0 cm, and the selected area in each test region is not less than 0.5 cm 2 Is irradiated with an ultraviolet ray solar simulator 1 time a day at a dose of 0.75 times MED for 4 days.
Skin color evaluation: on day 5 after the end of irradiation, each blackening test area was tested by a skin colorimeter and a skin melanin detector before the use of the product. And calculating the ITA DEG value by using the L, a and b color space values tested by the skin colorimeter, wherein the calculation formula is shown in a formula I. Wherein L represents brightness and b represents yellowness. The larger the ITA degree value is, the lighter the skin color is, otherwise, the darker the skin color is;
formula I.
Coating a test object: in the test zone, the concentration of the catalyst was controlled at (2.00.+ -. 0.05) mg/cm 2 The negative control area was not smeared with any product, but smeared with the test substances (skin care solutions obtained in examples and comparative examples). The product was applied at a frequency of 2 times/day (2 applications were spaced for a period of time not less than 4 h) and the test article was applied continuously for 4 weeks. Negative control: blackened area blank. After application, each darkened test area was visually assessed and instrumentally tested for skin color weekly and recorded and the results are shown in table 2.
TABLE 2 average values of skin metrics before and after use
Test example 3Acne removal test
Criteria for subject recruitment: criteria for group inclusion of test subjects: 18-25 years old, unlimited in sex; the face has several acnes.
Criteria for group exclusion of subjects: women during pregnancy or lactation are excluded according to clinical trial regulations; inflammatory skin patients; highly sensitive body constitution; patients with immunodeficiency or autoimmune disease; a diabetic patient; asthma or other chronic respiratory disease patients; record of immunosuppressant administration in the last month; for the last two months, any anti-inflammatory drugs are used for the acne part; the skin is not suitable for the tested part (such as fetal marks, scars and other flaws); other clinical trials are underway or have been conducted within the last 3 months; not voluntary participants and not able to complete the content on demand.
Personnel meeting the inclusion criteria must not use cosmetics at the test site on the test day and must not perform any other activities affecting the screening evaluation. The tested parts (forehead, right cheek and right mandible) are cleaned by clean water, and after sitting still for 30min in a constant temperature and humidity environment (temperature 22+/-1 ℃ and relative humidity 50% +/-10%). The subjects were instructed to apply the skin care solutions of examples and comparative examples to the affected area twice daily (morning and before sleeping, after facial cleansing) and return the subjects on day 28 of the product use. Parameters of the test site of the subject are tested, including skin surface moisture content, skin surface grease content, and number of acnes. Skin images were collected clinically using a VISIA-CR and analyzed for the ratio of area of subcutaneous porphyrin using IPP image analysis software, and the results are shown in table 3.
TABLE 3 average value of skin index changes before and after use
Claims (6)
1. The preparation method of the rosewood seed skin care lotion is characterized by comprising the following steps of:
under aseptic conditions, adding a culture medium into the rosewood seed extract, inoculating schizophyllum commune seed solution and ganoderma lucidum mycelia seed solution, fermenting until the fermentation is carried out to the early logarithmic phase, adding carbon quantum dots, fermenting until the fermentation is finished by intermittent ultraviolet irradiation, and carrying out solid-liquid separation to obtain a supernatant, namely the rosewood seed skin care solution;
wherein, the volume ratio of the rosewood seed leaching solution, the culture medium, the schizophyllum commune seed solution and the ganoderma lucidum mycelia seed solution is 800-1200: 1800-2200: 300-500: 300-500 parts;
the fermentation temperature is 25-30 ℃; the fermentation time is 120h;
the addition amount of the carbon quantum dots is 10 mg/L; the size of the carbon quantum dots is 1.8 nm;
the intermittent ultraviolet irradiation is specifically as follows: turning on an ultraviolet lamp in the fermentation tank every 0.5-2 hours to irradiate for 10-20 s until the fermentation is finished;
the culture medium is prepared by mixing the following raw materials: glucose 50 g/L, yeast extract 4g/L, potassium dihydrogen phosphate 0.5 g/L, magnesium sulfate heptahydrate 0.6 g/L, and vitamin B1 50 mg/L.
2. The preparation method according to claim 1, wherein the rosewood seed extract is prepared by the steps of:
1) Grinding and crushing rosewood seeds at low temperature, and screening to obtain rosewood seed powder;
2) Adding ethanol into rosewood seed powder for leaching, performing solid-liquid separation, and performing reduced pressure distillation on the obtained supernatant to obtain rosewood seed leaching solution.
3. The method according to claim 2, wherein,
in the step 1), the temperature of low-temperature grinding and crushing is-20 to-1 ℃.
4. The method according to claim 2, wherein,
in the step 2), the mass-volume ratio of the rosewood seed powder to the ethanol is 1:1-5; the leaching temperature is-10-1 ℃; the temperature of the reduced pressure distillation was 50 ℃.
5. The rosewood seed skin lotion prepared by the preparation method of any one of claims 1-4.
6. The use of rosewood seed lotion according to claim 5 in the preparation of a skin care product, wherein the skin care product has at least one of anti-wrinkle, whitening and anti-acne effects.
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