CN116508780A - Sterilization composition containing flumorph and application thereof - Google Patents

Abstract

The invention relates to the technical field of pesticide application, in particular to a bactericidal composition containing flumorph and application thereof, wherein the bactericidal composition comprises flumorph and cymoxanil as effective active ingredients, the weight ratio of flumorph to cymoxanil is 5:2-2:5, and a synergistic agent and an auxiliary agent are added into the bactericidal composition to prepare water dispersible granules. The bactericidal composition is used for preventing and treating potato late blight. In the early stage of the potato late blight, the stem and leaf is sprayed, and the medicine is applied for 3 times at intervals of 7-10 days, so that the potato late blight has a good treatment effect, is good in safety, has no adverse effect on other plant diseases and insect pests, and has no adverse effect on non-target organisms.

Description

Sterilization composition containing flumorph and application thereof

Technical Field

The invention belongs to the technical field of pesticide preparation and application, and in particular relates to a bactericidal composition containing flumorph and application thereof.

Background

The flumorph bactericide has the functions of protecting and treating, mainly inhibiting the biosynthesis of germ cell walls, and has good control effect on diseases generated by oomycete pathogenic bacteria, such as downy mildew, late blight, downy blight and the like.

Cymoxanil is a high-efficiency and low-toxicity bactericide, mainly preventing germ spore germination and hypha growth, and although it has no direct inhibition activity, it induces the disease-resistant action of plant itself, so as to prevent infection, and can attain the goal of finally eliminating germ.

The potato late blight mainly uses mycelium to hide in the potato to overwinter, and becomes an initial infection source of diseases in the next year. Seed potato with disease, the serious person can not sprout, or the person dies without sprouting; the light plants germinate and come out of the soil, and develop into central disease plants in the field. The dip dyeing is carried out by air flow and rainwater transmission. Most potato producing areas in China are suitable for late blight occurrence, so that humidity plays a role in determining diseases. The weather is moist, overcast and rainy, and the fog and dew are more in the morning and evening, which is beneficial to the onset and spread of diseases.

Late blight of potato is one of the main diseases of potato, which is common in most areas of middle and north China, and the loss degree of the disease varies with the climatic conditions of each area. Under the condition of proper disease epidemic, the plants die in advance, and 20-40% of yield loss can be caused. The leaves, stems and tubers of potatoes are mainly compromised, and symptoms generally appear before and after flowering.

The flumorph and cymoxanil are mixed in a proper proportion, so that a synergistic effect is generated on pathogenic bacteria, the generation of drug resistance of pathogenic bacteria can be delayed, the drug effect is improved, the dosage is reduced, and the economic cost is saved. Therefore, the mixed preparation of the flumorph and the cymoxanil in a proper proportion has positive significance and has popularization and application values in agricultural production.

Disclosure of Invention

The invention aims to provide a bactericidal composition containing flumorph and application thereof, wherein the bactericidal composition can effectively treat potato late blight, delay the generation of drug resistance of pathogenic bacteria, expand an antibacterial spectrum, improve drug effect, prolong the duration of time, reduce the dosage, save economic cost, have low toxicity and are safe to the environment.

The technical scheme of the invention is as follows:

a bactericidal composition containing flumorph and application thereof is characterized in that: the bactericidal composition comprises the effective active ingredients of flumorph and cymoxanil, wherein the weight ratio of flumorph to cymoxanil is 5:2-2:5, the preferred weight ratio is 2:3-2:5 or 3:2-5:2, and the optimal weight ratio is 2:5.

Further, the total weight of flumorph and cymoxanil in the bactericidal composition is 20-70%, and the preferred total weight is 70%.

Further, the bactericidal composition is prepared from flumorph, cymoxanil, a synergistic agent and an auxiliary agent, and is prepared into water dispersible granules.

Further, the auxiliary agent is one or more selected from dispersing agents, wetting agents, binders, defoamers, PH regulators and fillers.

Further, the synergist is selected from one of polyethoxy modified silane and polyethoxy modified fatty alcohol mixture or alkoxy modified polysilicone.

The dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthyl sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalene sulfonic acid formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate;

the wetting agent is one or more selected from sodium dodecyl benzene sulfonate, sodium diisobutyl naphthalene sulfonate, sorbitan fatty acid ester, sulfodocyl sodium salt, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenyl ethylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrene phenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate;

the binder is one or more selected from water, starch, polyvinylpyrrolidone, polyethylene glycol, glucose and polyvinyl alcohol;

the PH regulator is one or more selected from glacial acetic acid, citric acid, light calcium, potassium dihydrogen phosphate and triethanolamine;

the defoamer is one or more selected from organic silicone, fatty alcohol and fatty acid;

the filler is selected from the group consisting of: white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, talcum powder, diatomite, starch and urea.

Further, the use of a bactericidal composition for controlling potato late blight.

Further, the dosage of the active ingredient of the bactericidal composition is 200-350 g/ha, and the preferred dosage is 262.5-315 g/ha.

Further, the fungicidal composition is sprayed uniformly on all leaves of plants, the amount of applied liquid is 600 to 900 liters per hectare, and the preferable water consumption is 675 to 750 liters per hectare.

The bactericidal composition comprises the following components in percentage by weight when being prepared into water dispersible granules: 8 to 50 percent of flumorph, 8 to 50 percent of cymoxanil, 1 to 8 percent of synergistic agent, 1 to 15 percent of dispersing agent, 1 to 12 percent of wetting agent, 1 to 20 percent of adhesive, 0 to 3 percent of defoaming agent, 0 to 4 percent of PH regulator and 100 percent of filler.

Compared with the prior art, the invention has the following advantages:

(1) The composition has more remarkable synergism for preventing and treating potato late blight, and remarkably improves the prevention and treatment effect;

(2) Compared with a single dose, the dosage and the times of pesticide are reduced, and the prevention and treatment cost is reduced;

(3) Can effectively treat resistant diseases, particularly in the onset period, can rapidly control the disease condition, can delay the generation of drug resistance of diseases, and has long lasting period;

(4) Low toxicity, environmental protection, safety to non-target crops and safety to environment;

(5) Enhancing plant immunity, enhancing photosynthesis of plant leaves, and having certain yield increasing effect.

Detailed Description

The invention is further illustrated by the following examples, which are by weight, but are not limited thereto.

Application example one: preparation example:

example 1 70% flumorph cymoxanil water dispersible granule

20% of flumorph raw material, 50% of cymoxanil raw material, 2% of polyethoxy modified silane and polyethoxy modified fatty alcohol mixture, 3% of alkyl naphthalene sulfonate, 3% of polycarboxylate, 4% of fatty acid sulfate, 2% of fatty acid sulfate, 5% of polyvinylpyrrolidone, 0.2% of silicone defoamer, 1% of water and 100% of starch, thus preparing 70% flumorph cymoxanil water dispersible granule.

Example 2 70% flumorph cymoxanil water dispersible granule

20% of flumorph raw material, 50% of cymoxanil raw material, 3% of alkoxy modified polysilicone, 5% of methyl naphthalene sulfonate formaldehyde condensate, 4% of butyl naphthalene sulfonate sodium, 3% of sodium dodecyl sulfate, 4% of glucose, 1% of water, 0.2% of fatty alcohol, 5% of starch and 100% of diatomite are added to prepare the 70% flumorph cymoxanil water dispersible granule.

Example 3 60% flumorph cymoxanil water dispersible granule

36% of flumorph raw material, 24% of cymoxanil raw material, 4% of alkoxy modified polysilicone, 2% of alkyl naphthalene sulfonate formaldehyde polymer, 4% of polycarboxylate, 3% of sodium dodecyl sulfate, 5% of alkyl glycoside, 0.5% of fatty alcohol, 4% of polyvinyl alcohol, 2% of light calcium, 1% of anhydrous citric acid and 100% of potassium sulfate, thus preparing the 60% flumorph cymoxanil water dispersible granule.

Example 4 35% flumorph cymoxanil water dispersible granule

The composition comprises 10% of flumorph raw material, 25% of cymoxanil raw material, 3% of a mixture of polyethoxy modified silane and polyethoxy modified fatty alcohol, 3% of EO-PO block polyether, 4% of fatty alcohol polyoxyethylene ether, 3% of polyvinyl alcohol, 0.2% of an organic silicone defoamer, 15% of diatomite and 100% of calcined kaolin, and is prepared into 35% flumorph cymoxanil water dispersible granules.

Example 5 20% flumorph cymoxanil Water dispersible granule

The 20% flumorph cymoxanil water dispersible granule is prepared by adding 8% of flumorph raw material, 12% of cymoxanil raw material, 3% of alkoxy modified polysilicone, 3% of alkylphenol ethoxylate formaldehyde condensate, 3% of alkyl succinic acid sulfonate, 3% of alkyl glycoside, 5% of polyethylene glycol, 0.1% of fatty acid, 10% of urea and 100% of ammonium sulfate.

Application example two: indoor toxicity determination of flumorph and cymoxanil mixed potato late blight

According to the standards of NY/T1156.13-2008 and NY/T1156.6-2006, the results of measuring the toxicity of flumorph and cymoxanil and the ratio of 1:1, 2:3, 2:5, 5:2 and 3:2 thereof to potato late blight bacteria by adopting a leaf blade method show that flumorph and cymoxanil in the 5 ratios show synergistic effects on potato late blight bacteria, and the EC50 values of flumorph and cymoxanil are 3.25, 3.07, 2.76, 3.18 and 3.17mg/L respectively, and the co-toxicity coefficients of flumorph and cymoxanil are 128.07, 132.12, 142.79, 138.68 and 134.84 respectively. Wherein the synergistic effect of the two components is most obvious when the two components are mixed in a ratio of 2:5. The experimental procedure was as follows:

1 purpose of test

The toxicity of flumorph and cymoxanil and the mixed combination of the flumorph and the cymoxanil in different proportions to potato late blight bacteria is measured indoors, the synergism is evaluated, the suitability of the flumorph and the cymoxanil is clear, and a scientific basis is provided for the research and development of the cymoxanil and cymoxanil mixture.

2 test conditions

2.1 test targets

Potato late blight bacteria (Phytophthora infestans (mont.) de Bary) were stored and supplied by the laboratory.

2.2 culture conditions

The culture condition of the target to be tested and the target after the test is that the temperature is 18-20 ℃ and the relative humidity is more than 95%.

2.3 instrumentation

An electronic ten-thousandth balance, a volumetric flask, a beaker, a pipette, a graduated cylinder, a culture dish, a biological microscope, a crop sprayer, an intelligent artificial climate incubator and the like.

3 test design

3.1 test Agents

95% of flumorph (flumorph); cymoxanil 98% original drug 3.2 medicament concentration design and solution preparation

Flumorph solution: weighing 0.0105g of 95% flumorph crude drug, dissolving the flumorph crude drug in 0.2mL of DMF, fixing the volume to 100mL by using sterile water containing 0.1% of Tween80 emulsifier, shaking uniformly to prepare 100mg/L mother liquor, and diluting the mother liquor into 40, 20, 10, 5, 2.5 and 1.25mg/L concentration by using sterile water containing 0.1% of Tween80 emulsifier for standby;

cymoxanil solution: weighing 0.0102g of cymoxanil 98% crude drug, dissolving with 0.2mL of DMF, sterilizing with sterile constant volume containing 0.1% Tween80 emulsifier to 100mL, shaking uniformly to prepare 100mg/L mother liquor, and diluting with sterile water to 40, 20, 10, 5, 2.5 and 1.25mg/L concentration for later use;

each mixed solution: respectively taking 10, 25 and 15mL of 100mg/L flumorph solution, respectively adding the solution into 10, 15, 25, 10 and 10mL of 100mg/L cymoxanil solution to prepare 100mg/L flumorph cymoxanil 1:1, 2:3, 2:5, 5:2 and 3:2 solutions, and diluting the solutions into 40, 20, 10, 5, 2.5 and 1.25mg/L solutions by using sterile water containing 0.1% Tween80 emulsifier for standby;

blank control solution: 0.2mL DMF was added 99.8mL sterile water containing 0.1% Tween80 emulsifier.

4 test method

The leaf blade method was used with reference to the pesticide indoor bioassay test guidelines NY/T1156.13-2008.

Preparing sporangium suspension: culturing potato late blight bacteria on oat culture medium until sporangia are generated, washing potato late blight bacteria spores with distilled water at 4 ℃, filtering with double-layer gauze to prepare sporangia suspension (the concentration is controlled to be 1 multiplied by 105 sporangia per milliliter), and storing for 0.5-3 hours at 4 ℃ for standby.

Uniformly spraying the prepared liquid medicine on the back of the blade, naturally air-drying the liquid medicine, upwards treating the back of the blade, and discharging the blade into a moisturizing box according to the treatment mark. 24 hours after the treatment with the agent, the back of the leaf is sprayed with the fresh sporangia suspension prepared, and the test is run with the treatment without agent as a blank, with 4 replicates per treatment and 10 leaves per replicate. After inoculation, the culture medium is covered with a dish cover and placed in an intelligent artificial climate incubator to be cultured under the conditions of continuous illumination/darkness for 12 hours alternation every day, the temperature is 18-20 ℃ and the relative humidity is more than 90%.

5 data investigation and statistical analysis

5.1 investigation method

And when the leaf rate of the blank control reaches more than 50%, the disease conditions of all treatments are investigated in a grading way.

Level 0: does not cause disease;

stage 1: the area of the disease spots accounts for less than 10% of the area of the whole leaf;

3 stages: the area of the disease spots accounts for 10% -25% of the area of the whole leaf;

5 stages: the area of the lesion accounts for 25% -50% of the area of the whole leaf;

7 stages: the area of the disease spots accounts for more than 50% of the area of the whole leaf;

stage 9: whole leaves withered.

According to the investigation data, the disease index of each treatment is calculated according to the formula (1) and the control effect is calculated according to the formula (2).

Wherein: x represents the disease index, ni represents the leaf number of each stage, i represents the relative grade value, and N represents the total leaf number of investigation.

Wherein: p represents the control effect, CK represents the disease index of the blank control, and PT represents the disease index of the medicament treatment.

5.2 method for evaluating synergy

The synergy of the drug combination is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960) by reference to a biological standard method NY/T1156.6-2006, namely CTC less than or equal to 80 is antagonism, CTC less than or equal to 80 is additive, CTC less than or equal to 120 is synergistic.

Drug combination Theory Toxicity Index (TTI) =toxicity index of agent a×percent (%) of agent a in the combination+toxicity index of agent b×percent (%)

5.3 statistical analysis of data

All experimental data were analyzed using DPS v9.50 statistical software. According to the control effect (%) of the test data, a virulence regression equation, a correlation coefficient (r) and EC are obtained ₅₀ (95% confidence limit).

6 results analysis and discussion

6.1 evaluation of drug efficacy

The toxicity measurement results of flumorph and cymoxanil and the mixed combination of the flumorph and the cymoxanil in different proportions on potato late blight bacteria are shown in table 1. As can be seen from Table 1, in the 1:1, 2:3, 2:5, 5:2, 3:2 combination of flumorph and cymoxanil, five ratios showed synergistic effect on potato late blight bacteria, EC thereof ₅₀ The values were 3.25, 3.07, 2.76, 3.18 and 3.17mg/L, respectively, and the co-toxicity coefficients were 128.07, 132.12, 142.79, 138.68 and 134.84, respectively. Wherein the synergistic effect of the two components is most obvious when the two components are mixed in a ratio of 2:5.

TABLE 1 toxicity determination results of flumorph and cymoxanil on late blight of potato

6.2 discussion and conclusion

Indoor biological test results show that the flumorph and cymoxanil are mixed according to the proportion of 1:1, 2:3, 2:5, 5:2 and 3:2 to show synergistic effect on potato late blight bacteria, wherein the synergistic effect of the flumorph and cymoxanil mixed according to the proportion of 2:5 is most obvious, so that the optimal proportion of the flumorph and cymoxanil mixed according to the proportion of 2:5 can be considered.

Application example three: examples 1 and 2 environmental impact measurement experiments

I entrust third party test units to carry out 10 tests such as a bee acute oral toxicity test, a bee acute contact toxicity test, a bird acute oral toxicity test, a fish acute toxicity test, a silkworm acute toxicity test, a flea acute activity inhibition test, an algae growth inhibition test, an earthworm acute toxicity test, a natural enemy trichogramma acute toxicity test, a natural enemy ladybug acute contact toxicity test and the like on the test examples 1-2, wherein the test results are as follows:

1) The bee acute oral toxicity test shows that the toxicity level is low.

2) Bee acute contact toxicity test, the toxicity level is low.

3) Acute oral toxicity test of birds, the toxicity grade is low.

4) Acute toxicity test of fish, the toxicity grade is low.

5) The acute toxicity test of silkworm shows that the toxicity grade is low.

6) The daphnia acute activity inhibition test has low toxicity level.

7) Algae growth inhibition test, toxicity grade is low toxicity.

8) And the toxicity grade of the earthworm acute toxicity test is low.

9) The natural enemy trichogramma acute toxicity test is low in risk.

10 Natural enemy ladybug acute contact toxicity test, low risk.

Application example four: EXAMPLES 1 to 2 toxicology assay experiments

I delegate the third party detection mechanism to conduct acute toxicity test on the embodiment 1-2, and the test result is as follows:

1) Acute oral toxicity tests show that the toxicity levels are low.

2) Acute percutaneous toxicity test, toxicity grade is low toxicity.

3) Eye irritation test, no irritation.

4) Skin irritation test, no irritation.

5) Skin sensitization test, no sensitization.

Application example five: example 1 efficacy test against potato late blight

The test refers to the pesticide field efficacy test criterion (one) GB/T17980.34-2000: the bactericide is used for preventing and treating potato late blight.

Test object: potato late blight Phytophthora infestans

1 test basic information

1.1 test name

Field efficacy test of 70% flumorph cymoxanil water dispersible granule for preventing and treating potato late blight

1.2 purpose of test

The control effect, the applicable dosage and the safety of the 70% flumorph cymoxanil water dispersible granule provided by the applicant on potato late blight are clear, and the basis is provided for registering and popularizing test medicaments.

2 environmental and facility cultivation conditions

2.1 test position

Chongqing city unitary sun soil family Miao nationality county.

2.2 test target conditions

Potato late blight (Phytophthora infestans).

2.3 testing crops, varieties and growth

The experimental crop is potato, and the test variety is Yushu No. 6. The planting row spacing is 70cm, the plant spacing is about 30cm, the planting density is moderate, and the growth environment is good. Potatoes are at the beginning of tuber formation when administered.

3 test design and arrangement

3.1 test amount and numbering

TABLE 2 test design of reagents for test

3.2 periods of application and methods

The drug was administered 1 time every 10 days (2021, 06, 14, 2021, 06, 24) at the beginning of the onset of potato late blight (2021, 06, 04), 3 times.

3.3 investigation method, time and times

3.3.1 investigation time and method

Crop safety investigation: the potato is observed 1, 3 and 5 days after each application to see whether the potato is harmful. And (5) investigating whether each part of the plant has obvious phytotoxicity symptoms such as fading, burning, spotting and the like. And simultaneously, the influence of the test agent on field non-target organisms such as wild animals, beneficial insects, other diseases and insect pests and the like is investigated.

Disease investigation: first investigation of the occurrence of potato plant late blight in each cell before the second application (2021, 14, 06); a second investigation of the occurrence of district potato late blight before the third application (24 days of 2021, 06); the disease occurrence was investigated for the third time 10d (2021, 07, 04) after the third application, for a total of 3 times.

Sampling five diagonal points in each cell, taking 2 plants in each point, investigating all leaves, and recording according to the following grading method:

level 0: no disease spots;

stage 1: the area of the disease spots accounts for less than 5% of the whole blade area;

3 stages: the area of the disease spots accounts for 6% -10% of the whole blade area;

5 stages: the area of the disease spots accounts for 11% -20% of the whole blade area;

7 stages: the area of the disease spots accounts for 21% -50% of the whole blade area;

stage 9: the area of the disease spots accounts for more than 50% of the whole blade area.

3.3.3 method for calculating efficacy

Statistical analysis of the test data was performed by SPSS 25.0 using the new complex polar difference method of duncan.

4 results and analysis

TABLE 3 test results of 70% flumorph cymoxanil water dispersible granule for controlling potato late blight (1)

TABLE 4 test results of 70% flumorph cymoxanil water dispersible granule for controlling potato late blight (2)

TABLE 5 test results of 70% flumorph cymoxanil water dispersible granule for controlling potato late blight (3)

The results of three surveys in the test agent treatment area of the 70% flumorph cymoxanil water dispersible granule with the dosage of the effective components of 262.5-315 g/hectare in the example 1 show that the morbidity and the disease index are obviously lower than those of the control agent treatment, the morbidity of the survey results after 10 days after the third administration is 8.48% and 5.58%, and the disease index is 2.00 and 1.38, respectively; the control effect is obviously higher than that of the control agent treatment, the control effect of 262.5 g/hectare treatment for three surveys is 71.41%, 76.31% and 80.60%, and the control effect of 315 g/hectare treatment for three surveys is 76.00%, 81.95% and 86.59%, respectively.

The test result shows that the test agent has obvious inhibition effect on the disease condition of the potato late blight by carrying out foliar spraying for 3 times at 7-10 days in the initial period of the disease occurrence of the potato late blight by using 262.5-315 g/hectare of the effective component dosage of the flumorph cymoxanil water dispersible granule, the disease index of a treatment cell is obviously reduced compared with that of a clear water treatment cell, and the treatment cell has no adverse effect on other non-target organisms.

Application example six: example 2 efficacy test against potato late blight

Specific experimental basis, experimental medicament and investigation method refer to application example five.

1 test position

Shanxi province of salt-Yang city.

2 test crop, variety and growth conditions

Cucumber is the potato No. 5, and grows well.

3 method of administering a drug

3.1 periods of application and methods

The potato late blight is treated by adopting a plant stem and leaf uniform spraying method in the early stage of the potato late blight. The test was performed with the first administration at 2021, 6 months, 23 days, the second administration at 6 months, 30 days, the third administration at 7 months, 7 days, and a total of 3 administrations.

3.2 usage Capacity

The amount of the chemical solution sprayed per hectare was about 750 liters.

3.3 investigation time

The disease number was investigated before administration at 2021, 6 and 23, and then the control effect was investigated 10 days after the last administration (at 2021, 7 and 17), 2 times in total.

3.4 method for calculating efficacy

Wherein: CK0 and CK1 are disease indexes before and after administration in a blank control area, and PT0 and PT1 are disease indexes before and after administration in a medicament treatment area.

4 results and analysis

Table 6 test results of 70% flumorph cymoxanil water dispersible granule for preventing and treating potato late blight

As can be seen from table 6, the 70% flumorph cymoxanil water dispersible granule of example 2 has a better control effect on potato late blight, and the average control effects of 30 g/mu and 25 g/mu treatment after the last treatment for 10 days are 83.51% and 78.55% respectively; the average control effect of the control medicament 20% cymoxanil suspending agent 100 ml/mu treatment and the 30% flumorph suspending agent 45 ml/mu treatment after 10 days of last medicament is 75.55% and 75.34%.

The control effect 10 days after the last medicine is subjected to biological analysis by adopting a Duncan new complex polar Difference (DMRT) method, and the analysis of variance shows that: the inter-treatment variability reached a very significant level, with no significant inter-repeat variability. The difference comparison result shows that: at the 0.05 level and the 0.01 level, the difference significance comparison results were consistent. The difference between the test agent 70% flumorph cymoxanil water dispersible granule 30 g/mu treatment and other treatments reaches a very significant level; the difference between the test agent 70% flumorph cymoxanil water dispersible granule treatment of 25 g/mu and other treatments reaches a very significant level; the difference between the control medicament 20% cymoxanil suspending agent 100 ml/mu treatment and the control medicament 30% flumorph suspending agent 45 ml/mu treatment is not obvious, but the difference between the control medicament 20% cymoxanil suspending agent 100 ml/mu treatment and the control medicament 30% flumorph suspending agent 45 ml/mu treatment reaches a very obvious level.

The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several simple deductions or substitutions may be made without departing from the spirit of the invention, and these should be considered to be within the scope of the invention.

Claims (9)

1. A bactericidal composition containing flumorph and application thereof is characterized in that: the bactericidal composition comprises the effective active ingredients of flumorph and cymoxanil, wherein the weight ratio of flumorph to cymoxanil is 5:2-2:5.

2. The bactericidal composition of claim 1 wherein: the weight ratio of flumorph to cymoxanil is 2:3-2:5 or 3:2-5:2.

3. The bactericidal composition of claim 2 wherein: the weight ratio of flumorph to cymoxanil is 2:5.

4. The bactericidal composition of claim 1 wherein: the total weight of flumorph and cymoxanil in the bactericidal composition is 20% -70%.

5. The bactericidal composition of claim 4 wherein: the total weight of flumorph and cymoxanil in the bactericidal composition is 70 percent.

6. The bactericidal composition of any of claims 1-5, wherein: the bactericidal composition is prepared from flumorph, cymoxanil, a synergistic agent and an auxiliary agent and is prepared into water dispersible granules.

7. The bactericidal composition of claim 6 wherein: the auxiliary agent is one or more selected from dispersing agent, wetting agent, binding agent, defoamer, PH regulator and filler.

8. Use according to claim 1, characterized in that: use of a bactericidal composition for controlling potato late blight.

9. Use according to claim 8, characterized in that: the dosage of the active ingredients of the bactericidal composition is 200-350 g/hectare.