CN116492284A - 美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 - Google Patents
美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 Download PDFInfo
- Publication number
- CN116492284A CN116492284A CN202310721864.1A CN202310721864A CN116492284A CN 116492284 A CN116492284 A CN 116492284A CN 202310721864 A CN202310721864 A CN 202310721864A CN 116492284 A CN116492284 A CN 116492284A
- Authority
- CN
- China
- Prior art keywords
- parts
- extract
- lactobacillus casei
- fermented
- ferment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229940017800 lactobacillus casei Drugs 0.000 title claims abstract description 59
- 244000199866 Lactobacillus casei Species 0.000 title claims abstract description 58
- 235000013958 Lactobacillus casei Nutrition 0.000 title claims abstract description 58
- 238000000855 fermentation Methods 0.000 title claims abstract description 27
- 230000004151 fermentation Effects 0.000 title claims abstract description 27
- 230000002087 whitening effect Effects 0.000 title claims abstract description 19
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 17
- 230000008099 melanin synthesis Effects 0.000 claims abstract description 9
- 238000004321 preservation Methods 0.000 claims abstract description 7
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- 244000005700 microbiome Species 0.000 claims abstract description 4
- 239000000284 extract Substances 0.000 claims description 63
- 230000001954 sterilising effect Effects 0.000 claims description 20
- 239000002054 inoculum Substances 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 12
- 241001506047 Tremella Species 0.000 claims description 10
- 239000011268 mixed slurry Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 241001313857 Bletilla striata Species 0.000 claims description 8
- 244000197580 Poria cocos Species 0.000 claims description 8
- 235000008599 Poria cocos Nutrition 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 235000012907 honey Nutrition 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 244000303040 Glycyrrhiza glabra Species 0.000 claims description 7
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 7
- 229940069521 aloe extract Drugs 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 239000008213 purified water Substances 0.000 claims description 7
- 229940119485 safflower extract Drugs 0.000 claims description 7
- 241000045403 Astragalus propinquus Species 0.000 claims description 6
- 235000006533 astragalus Nutrition 0.000 claims description 6
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 6
- 238000009630 liquid culture Methods 0.000 claims description 6
- 235000011477 liquorice Nutrition 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- 235000006484 Paeonia officinalis Nutrition 0.000 claims description 5
- 241001633680 Polygonatum odoratum Species 0.000 claims description 5
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 claims description 2
- 244000170916 Paeonia officinalis Species 0.000 claims 1
- 241000304195 Salvia miltiorrhiza Species 0.000 claims 1
- 230000001085 cytostatic effect Effects 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 14
- 230000002401 inhibitory effect Effects 0.000 abstract description 12
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 239000006071 cream Substances 0.000 abstract description 2
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 230000001815 facial effect Effects 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 239000000047 product Substances 0.000 description 40
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 24
- 210000003491 skin Anatomy 0.000 description 17
- 241000186605 Lactobacillus paracasei Species 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 230000001737 promoting effect Effects 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 241001106477 Paeoniaceae Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 102000003425 Tyrosinase Human genes 0.000 description 3
- 108060008724 Tyrosinase Proteins 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000002216 heart Anatomy 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 244000132619 red sage Species 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 229940126680 traditional chinese medicines Drugs 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 102000011782 Keratins Human genes 0.000 description 2
- 108010076876 Keratins Proteins 0.000 description 2
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- 208000003351 Melanosis Diseases 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 240000007164 Salvia officinalis Species 0.000 description 2
- 206010040914 Skin reaction Diseases 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- 230000007803 itching Effects 0.000 description 2
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 2
- 229960004705 kojic acid Drugs 0.000 description 2
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000035483 skin reaction Effects 0.000 description 2
- 231100000430 skin reaction Toxicity 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 description 2
- 239000001393 triammonium citrate Substances 0.000 description 2
- 235000011046 triammonium citrate Nutrition 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 208000001382 Experimental Melanoma Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000017443 Hedysarum boreale Nutrition 0.000 description 1
- 235000007858 Hedysarum occidentale Nutrition 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 244000236658 Paeonia lactiflora Species 0.000 description 1
- 235000008598 Paeonia lactiflora Nutrition 0.000 description 1
- 241000756042 Polygonatum Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 206010039424 Salivary hypersecretion Diseases 0.000 description 1
- 235000017276 Salvia Nutrition 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 206010042496 Sunburn Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 150000003862 amino acid derivatives Chemical class 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000019206 astragalus extract Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011362 coarse particle Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000007933 dermal patch Substances 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- PSLIMVZEAPALCD-UHFFFAOYSA-N ethanol;ethoxyethane Chemical compound CCO.CCOCC PSLIMVZEAPALCD-UHFFFAOYSA-N 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229940069445 licorice extract Drugs 0.000 description 1
- -1 masks Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 235000005412 red sage Nutrition 0.000 description 1
- 208000026451 salivation Diseases 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/245—Lactobacillus casei
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oncology (AREA)
- Pharmacology & Pharmacy (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明涉及微生物技术领域，具体涉及一种美白抗菌的干酪乳杆菌SF‑L‑12发酵物及其应用。干酪乳杆菌SF‑L‑12发酵物由被发酵底物经干酪乳杆菌（Lactobacillus casei）SF‑L‑12发酵而得，所述干酪乳杆菌SF‑L‑12于2020年10月21日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为北京市朝阳区北辰西路1号院3号，保藏编号为CGMCC No.20927。该干酪乳杆菌SF‑L‑12发酵物具有优异的抗菌功能和较高的抑制黑色素合成效果，可用于制备具有美白功效的面膜、面霜、精华液、爽肤水等护肤产品，安全无毒副作用。
Description
技术领域
本发明涉及微生物技术领域，具体涉及一种美白抗菌的干酪乳杆菌SF-L-12发酵物及其应用。
背景技术
随着审美趋势的变化，美白提亮、均匀肤色成为当下关注的热点，而皮肤的白亮程度和皮肤中的黑色素密不可分。黑色素是一种氨基酸衍生物，它们存在于皮肤基底层的细胞中间，此时并不是真正意义上的黑色素，而是一种叫“黑色素原生物质”的物质。色素母细胞分泌麦拉宁色素，当紫外线（B波、A波）照射到皮肤上，肌肤就会处于“自我防护”的状态，即紫外线刺激色素母细胞分泌麦拉宁色素，激活酪氨酸酶的活性，来保护我们的皮肤细胞。酪氨酸酶与血液中的酪氨酸反应，生成一种叫“多巴”的物质。多巴其实就是黑色素的前身，经酪氨酸氧化而成，释放出黑色素。黑色素又经由细胞代谢的层层移动，到了肌肤表皮层形成雀斑、晒斑、黑斑等。因此，很多美白护肤产品通过以下一种或多种途径实现美白功效：抑制黑色素细胞增殖、抑制黑色素形成、抑制黑色素颗粒转移至角朊细胞、加速角朊细胞向角质层转移或减少如紫外线等外源性因素的影响。但是常见的美白护肤产品存在效果不稳定或会对皮肤产生刺激和副作用等缺点，如维生素C效果不稳定；熊果苷安全，但对酪氨酸酶抑制效应缓慢；曲酸美白祛斑效果明显，但有一定毒性。
发明内容
针对现有美白护肤产品存在效果不稳定或会对皮肤产生刺激和副作用等缺点的技术问题，本发明提供一种美白抗菌的干酪乳杆菌SF-L-12发酵物及其应用，运用生物发酵技术，原料全部采用药食同源性中药，安全无毒，具有良好的抗菌功能，促进皮肤排毒，抑制黑色素、色斑形成，达到美白护肤的功效。
第一方面，本发明提供一种美白抗菌的干酪乳杆菌SF-L-12发酵物及其应用，所述干酪乳杆菌（Lactobacillus casei）SF-L-12，于2020年10月21日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为北京市朝阳区北辰西路1号院3号，保藏编号为CGMCC No.20927，相关信息已公开于中国专利CN113444675B。
所述干酪乳杆菌SF-L-12发酵物的被发酵底物包括如下重量份的组分：净化水50~75份，白芷提取物1~3份，茯苓提取物2~4份，白芍提取物1~3份，红花提取物3~5份，白芨提取物2~4份，天门冬提取物2~4份，丹参提取物1~3份，甘草提取物3~5份，芦荟提取物2~4份，黄芪提取物2~4份，玉竹提取物2~4份，银耳粉2~4份，蜂蜜2~ 4份。
进一步的，所述被发酵底物包括如下重量份的组分：净化水62份，白芷提取物2份，茯苓提取物3份，白芍提取物2份，红花提取物4份，白芨提取物3份，天门冬提取物3份，丹参提取物2份，甘草提取物4份，芦荟提取物3份，黄芪提取物3份，玉竹提取物3份，银耳粉3份，蜂蜜3份。
被发酵底物的各组分具备如下功效：
白芷：解表散寒，祛风止痛，通鼻窍，燥湿止带，消肿排脓，祛风止痒；
茯苓：味甘、淡，性平；归心、肺、脾、肾经，利水渗湿，健脾，宁心；
白芍：养血调经，敛阴止汗，柔肝止痛，平抑肝阳；
红花：活血通经，去瘀止痛；
白芨：收敛止血，消肿生肌；
天门冬：滋阴，润燥，清肺，降火；
丹参：活血祛瘀，通经止痛，清心除烦，凉血消痈；
甘草：补脾益气，清热解毒，祛痰止咳，缓急止痛，调和诸药；
芦荟：滋润皮肤，保湿，消炎止痒；
黄芪：补气固表，托毒排脓，利尿，生肌；
玉竹：养阴润燥，生津止渴；
银耳：滋补生津；润肺养胃；
蜂蜜：调补脾胃。
被发酵物底物全部采用药食同源性中药，安全无毒。
进一步的，所述干酪乳杆菌SF-L-12发酵物中含有干酪乳杆菌SF-L-12的总数量为10~30亿/ml。
进一步的，其制备方法具体包括以下步骤：
（1）首先将被发酵底物的各组分混合，得到混合浆料；
（2）将混合浆料输送至灭菌容器中，高压灭菌，冷却后得到灭菌液；
（3）将干酪乳杆菌SF-L-12接种到MRS液体培养基中，培养得到干酪乳杆菌SF-L-12接种剂；
（4）将干酪乳杆菌SF-L-12接种剂接种于灭菌液中，培养得到发酵液；
（5）将发酵液输送至高压喷雾破壁机中进行破壁处理，得到破壁发酵液；
（6）破壁发酵液进行巴氏灭菌，得干酪乳杆菌SF-L-12发酵物。
进一步的，所述步骤（2）中高压灭菌的条件为：120℃高压灭菌30min，冷却终点温度为35℃。
进一步的，所述步骤（3）中，接种量为1%，培养温度为37℃，培养时间为18h。
进一步的，所述步骤（4）中，接种量为1%，培养温度为37℃，培养时间为24h。
进一步的，所述步骤（5）中，高压喷雾破壁机的压力设置为60Mpa。
第二方面，本发明还提供上述干酪乳杆菌SF-L-12发酵物在制备具有抗菌、抑制细胞黑色素合成功效的护肤产品方面的应用。
本发明的有益效果在于：
经试验验证，本发明提供的具有美白抗菌功能的干酪乳杆菌SF-L-12发酵物，具有优异的抗菌功能和较高的抑制黑色素合成效果，可用于制备具有美白功效的面膜、面霜、精华液、爽肤水等护肤产品，原料为药食同源性中药，安全无毒副作用。
具体实施方式
为了使本技术领域的人员更好地理解本发明中的技术方案，下面将对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都应当属于本发明保护的范围。
本发明使用的MRS固体平板培养基包括以下组分：蛋白胨10g、牛肉粉5g、酵母粉4g、K2HPO4•7H2O 2g、柠檬酸三铵2g、葡萄糖20g、CH3COONa•3H2O 5g、MgSO4•7H2O 0.2g、MnSO4•4H2O 0.05g、吐温80 1mL、琼脂15.0g、蒸馏水1000mL。
本发明使用的MRS液体培养基包括以下组分：蛋白胨10g、牛肉粉5g、酵母粉4g、K2HPO4•7H2O 2g、柠檬酸三铵2g、葡萄糖20g、CH3COONa•3H2O 5g、MgSO4•7H2O 0.2g、MnSO4•4H2O 0.05g、吐温80 1mL、蒸馏水1000mL。
本发明使用的各物质提取物的制备方法为：将原料洗净后破碎成粗粒，用10倍量水煎煮2次，煎煮时间为3h和1h，合并两次滤液，静置2h后过滤得提取液。将提取液在真空度为-0.06~0.08Mpa浓缩至相对密度为1.10（70℃），然后进行喷雾干燥，最后粉碎成80目细粉即得提取物。
银耳粉的制备方法为：将银耳烘干至含水量5%以下，磨碎成80目的银耳粉，待用。
本发明使用的干酪乳杆菌SF-L-12为发明人分离所得，已于2020年10月21日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为北京市朝阳区北辰西路1号院3号，保藏编号为CGMCC NO.20927，相关信息已公开于中国专利CN113444675B。
本发明使用的类干酪乳杆菌CICC 20251、干酪乳杆菌ACCC 10640及其他未作特殊说明的实验试剂均从商业途径购得。
实施例1一种干酪乳杆菌SF-L-12发酵物及其制备方法
首先准备被发酵底物：净化水62份，白芷提取物2份，茯苓提取物3份，白芍提取物2份，红花提取物4份，白芨提取物3份，天门冬提取物3份，丹参提取物2份，甘草提取物4份，芦荟提取物3份，黄芪提取物3份，玉竹提取物3份，银耳粉3份，蜂蜜3份。
按照以下工艺制备：（1）首先将被发酵底物的各组分混合，得到混合浆料；（2）将混合浆料输送至灭菌容器中，120℃高压灭菌30min，然后冷却至35℃，得到灭菌液；（3）将干酪乳杆菌SF-L-12接种到MRS液体培养基中，接种量为1%，37℃下培养18h得干酪乳杆菌SF-L-12接种剂；（4）将干酪乳杆菌SF-L-12接种剂接种于灭菌液中，接种量为1%，37℃下培养24h，得到发酵液；（5）将发酵液输送至高压喷雾破壁机中，压力设置60MPa，进行破壁处理，得到破壁发酵液；（6）破壁发酵液进行巴氏灭菌，得干酪乳杆菌SF-L-12发酵物。
实施例2一种干酪乳杆菌SF-L-12发酵物及其制备方法
首先准备被发酵底物：净化水75份，白芷提取物1份，茯苓提取物2份，白芍提取物1份，红花提取物3份，白芨提取物2份，天门冬提取物2份，丹参提取物1份，甘草提取物3份，芦荟提取物2份，黄芪提取物2份，玉竹提取物2份，银耳粉2份，蜂蜜2份。
按照实施例1工艺制备干酪乳杆菌SF-L-12发酵物。
实施例3一种干酪乳杆菌SF-L-12发酵物及其制备方法
首先准备被发酵底物：净化水50份，白芷提取物3份，茯苓提取物4份，白芍提取物3份，红花提取物5份，白芨提取物4份，天门冬提取物4份，丹参提取物3份，甘草提取物5份，芦荟提取物4份，黄芪提取物4份，玉竹提取物4份，银耳粉4份，蜂蜜4份。
按照实施例1工艺制备干酪乳杆菌SF-L-12发酵物。
对比例1一种类干酪乳杆菌CICC 20251发酵物及其制备方法
首先准备与实施例1相同的被发酵底物，然后按照以下工艺制备：（1）首先将被发酵底物的各组分混合，得到混合浆料；（2）将混合浆料输送至灭菌容器中，120℃高压灭菌30min，然后冷却至35℃，得到灭菌液；（3）将类干酪乳杆菌CICC 20251接种到MRS液体培养基中，接种量为1%，37℃下培养18h得类干酪乳杆菌CICC 20251接种剂；（4）将类干酪乳杆菌CICC 20251接种剂接种于灭菌液中，接种量为1%，37℃下培养24h，得到发酵液；（5）将发酵液输送至高压喷雾破壁机中，压力设置60MPa，进行破壁处理，得到破壁发酵液；（6）破壁发酵液进行巴氏灭菌，得类干酪乳杆菌CICC 20251发酵物。
对比例2一种干酪乳杆菌ACCC 10640发酵物及其制备方法
首先准备与实施例1相同的被发酵底物，按照以下工艺制备：（1）首先将被发酵底物的各组分混合，得到混合浆料；（2）将混合浆料输送至灭菌容器中，120℃高压灭菌30min，然后冷却至35℃，得到灭菌液；（3）将干酪乳杆菌ACCC 10640接种到MRS液体培养基中，接种量为1%，37℃下培养18h得干酪乳杆菌ACCC 10640接种剂；（4）将干酪乳杆菌ACCC 10640接种剂接种于灭菌液中，接种量为1%，37℃下培养24h，得到发酵液；（5）将发酵液输送至高压喷雾破壁机中，压力设置60MPa，进行破壁处理，得到破壁发酵液；（6）破壁发酵液进行巴氏灭菌，得干酪乳杆菌ACCC 10640发酵物。
测试例1发酵物抑菌能力测试
将实施例1-3、对比例1-2制备的发酵物经0.22μm无菌滤膜过滤，得发酵上清液。活化2代的金黄色葡萄球菌菌悬液调整为1×108CFU/mL，取100μL涂布于哥伦比亚血平板，牛津杯中加入150μL发酵上清液，对照组添加等量的蒸馏水，正置于三气培养箱中培养24h，测定抑菌圈直径，结果如表1所示。
表1各组发酵物抑菌能力结果
通过以上抑菌能力测试发现，上述干酪乳杆菌SF-L-12、类干酪乳杆菌CICC20251、干酪乳杆菌ACCC 10640发酵后的产物均具有一定的抑菌能力，但是干酪乳杆菌SF-L-12发酵物的抑菌能力明显优于类干酪乳杆菌CICC 20251发酵物、干酪乳杆菌ACCC 10640发酵物的抑菌能力，其中实施例1的发酵物抑菌能力较其他实施例发酵物抑菌能力强。
测试例2干酪乳杆菌SF-L-12发酵物人体安全性评价
人体皮肤斑贴试验：选取30名符合要求的志愿者作为受试对象，将实施例1-3制备的发酵物放入斑试器小室内，用量约为0.02ml，将加有发酵物的斑试器用低致敏胶带贴敷于受试者的前臂曲侧，用手掌轻压使之均匀地贴敷于皮肤上，持续24h。分别于去除受试物斑贴器后30min（待压痕消失后）、24h和48h按表2标准观察皮肤反应，并记录观察结果。
表2 各种皮肤反应及结果判断
结果显示：实施例1-3的试验组结果全部为阴性。
结论：本发明发酵物作为外敷原料，符合国家化妆品要求，安全性高。
测试例3B16细胞黑色素合成抑制率检测
B16黑色素瘤细胞使用10%小牛血清培养液培养，换代培养时，转接细胞浓度为3×106个/L。B16细胞培养48h后，更换含有0.1%浓度待检样品的培养基继续培养，以无添加培养基为空白对照。试验组分为实施例1组、实施例2组、实施例3组、对比例1组、对比例2组，每种待检样品设3个生物学重复。继续培养48h后取细胞5~10×106个，使用PBS缓冲液（0.1M，pH7.2）洗涤2次后重悬于200μL双蒸水中，1mL乙醇–乙醚混合液室温静置处理，20min后离心取沉淀重悬于1mLNaOH中，80℃水浴30min，测定A490值，由此计算黑色素合成抑制率，计算公式如下：黑色素合成抑制率(%)=（空白组A490值－试验组A490值）/空白组A490值×100。结果如表3所示：
表3 各组发酵物黑色素合成抑制率
测定结果统计分析显示，各组发酵物对B16黑色素合成抑制均有效果，但是干酪乳杆菌SF-L-12发酵物对B16黑色素合成的抑制效果明显优于类干酪乳杆菌CICC 20251发酵物、干酪乳杆菌ACCC 10640发酵物，其中实施例1效果最为明显，对黑色素合成抑制率达到37%以上，与市面上常见的黑色素抑制组分（如曲酸、VC等）效果相当。
尽管通过优选实施例的方式对本发明进行了详细描述，但本发明并不限于此。在不脱离本发明的精神和实质的前提下，本领域普通技术人员可以对本发明的实施例进行各种等效的修改或替换，而这些修改或替换都应在本发明的涵盖范围内/任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，可轻易想到变化或替换，都应涵盖在本发明的保护范围之内。
Claims (8)
1. 一种美白抗菌的干酪乳杆菌SF-L-12发酵物，其特征在于，所述干酪乳杆菌SF-L-12发酵物的被发酵底物包括如下重量份的组分：净化水50~75份，白芷提取物1~3份，茯苓提取物2~4份，白芍提取物1~3份，红花提取物3~5份，白芨提取物2~4份，天门冬提取物2~4份，丹参提取物1~3份，甘草提取物3~5份，芦荟提取物2~4份，黄芪提取物2~4份，玉竹提取物2~4份，银耳粉2~4份，蜂蜜2~4份；所述干酪乳杆菌（Lactobacillus casei）SF-L-12，于2020年10月21日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为北京市朝阳区北辰西路1号院3号，保藏编号为CGMCC No.20927。
2.如权利要求1所述的干酪乳杆菌SF-L-12发酵物，其特征在于，所述被发酵底物包括如下重量份的组分：净化水62份，白芷提取物2份，茯苓提取物3份，白芍提取物2份，红花提取物4份，白芨提取物3份，天门冬提取物3份，丹参提取物2份，甘草提取物4份，芦荟提取物3份，黄芪提取物3份，玉竹提取物3份，银耳粉3份，蜂蜜3份。
3.如权利要求1所述的干酪乳杆菌SF-L-12发酵物，其特征在于，其制备方法具体包括以下步骤：
（1）首先将被发酵底物的各组分混合，得到混合浆料；
（2）将混合浆料输送至灭菌容器中，高压灭菌，冷却后得到灭菌液；
（3）将干酪乳杆菌SF-L-12接种到MRS液体培养基中，培养得到干酪乳杆菌SF-L-12接种剂；
（4）将干酪乳杆菌SF-L-12接种剂接种于灭菌液中，培养得到发酵液；
（5）将发酵液输送至高压喷雾破壁机中进行破壁处理，得到破壁发酵液；
（6）破壁发酵液进行巴氏灭菌，得干酪乳杆菌SF-L-12发酵物。
4.如权利要求3所述的干酪乳杆菌SF-L-12发酵物，其特征在于，所述步骤（2）中高压灭菌的条件为：120℃高压灭菌30min，冷却终点温度为35℃。
5.如权利要求3所述的干酪乳杆菌SF-L-12发酵物，其特征在于，所述步骤（3）中，接种量为1%，培养温度为37℃，培养时间为18h。
6.如权利要求3所述的干酪乳杆菌SF-L-12发酵物，其特征在于，所述步骤（4）中，接种量为1%，培养温度为37℃，培养时间为24h。
7.如权利要求3所述的干酪乳杆菌SF-L-12发酵物，其特征在于，所述步骤（5）中，高压喷雾破壁机的压力设置为60Mpa。
8.如权利要求1-7任一项所述干酪乳杆菌SF-L-12发酵物在制备具有抗菌、抑制细胞黑色素合成功效的护肤产品方面的应用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310721864.1A CN116492284A (zh) | 2023-06-19 | 2023-06-19 | 美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310721864.1A CN116492284A (zh) | 2023-06-19 | 2023-06-19 | 美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116492284A true CN116492284A (zh) | 2023-07-28 |
Family
ID=87323296
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310721864.1A Pending CN116492284A (zh) | 2023-06-19 | 2023-06-19 | 美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116492284A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089580A (zh) * | 2023-10-19 | 2023-11-21 | 山东向日葵生物工程有限公司 | 干酪乳杆菌sf-l-12发酵物及其制备工艺、应用 |
| CN117085079A (zh) * | 2023-10-20 | 2023-11-21 | 妙越缇卡生物医药科技(山东)有限公司 | 植物乳杆菌sf-l38的发酵制品及其制备方法和应用 |
| CN117646038A (zh) * | 2024-01-30 | 2024-03-05 | 妙越缇卡生物医药科技(山东)有限公司 | 美白淡斑的副干酪乳杆菌sf-l31发酵物及其应用 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494302A (zh) * | 2020-06-12 | 2020-08-07 | 洋浦吉商生物科技有限公司 | 益生元美白抗衰老发酵化妆品 |
| CN113444675A (zh) * | 2021-09-01 | 2021-09-28 | 山东向日葵生物工程有限公司 | 一种干酪乳杆菌sf-l-12及其植物饮品和在卵巢保养中的应用 |
| CN113559022A (zh) * | 2021-07-30 | 2021-10-29 | 徐晓英 | 一种用益生菌发酵中药“新七白”的发酵方法 |
| CN115154393A (zh) * | 2022-07-04 | 2022-10-11 | 完美（广东）日用品有限公司 | 一种芦荟发酵液及其制备方法与应用 |
| CN115671007A (zh) * | 2022-12-30 | 2023-02-03 | 花安堂生物科技集团有限公司 | 红景天光甘草定复合发酵液、含其产品、及其制备和应用 |
-
2023
- 2023-06-19 CN CN202310721864.1A patent/CN116492284A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111494302A (zh) * | 2020-06-12 | 2020-08-07 | 洋浦吉商生物科技有限公司 | 益生元美白抗衰老发酵化妆品 |
| CN113559022A (zh) * | 2021-07-30 | 2021-10-29 | 徐晓英 | 一种用益生菌发酵中药“新七白”的发酵方法 |
| CN113444675A (zh) * | 2021-09-01 | 2021-09-28 | 山东向日葵生物工程有限公司 | 一种干酪乳杆菌sf-l-12及其植物饮品和在卵巢保养中的应用 |
| CN115154393A (zh) * | 2022-07-04 | 2022-10-11 | 完美（广东）日用品有限公司 | 一种芦荟发酵液及其制备方法与应用 |
| CN115671007A (zh) * | 2022-12-30 | 2023-02-03 | 花安堂生物科技集团有限公司 | 红景天光甘草定复合发酵液、含其产品、及其制备和应用 |
Non-Patent Citations (2)
| Title |
|---|
| 国家药监局: "国家药监局关于更新化妆品禁用原料目录的公告（2021年第74号）", pages 1 - 6, Retrieved from the Internet <URL:国家药品监督管理局网站> * |
| 戴玥，余晓斌: "抗氧化菌的筛选及混菌发酵红景天条件优化", 日用化学工业, vol. 47, no. 7, pages 408 - 413 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117089580A (zh) * | 2023-10-19 | 2023-11-21 | 山东向日葵生物工程有限公司 | 干酪乳杆菌sf-l-12发酵物及其制备工艺、应用 |
| CN117089580B (zh) * | 2023-10-19 | 2024-02-02 | 山东向日葵生物工程有限公司 | 干酪乳杆菌sf-l-12发酵物及其制备工艺、应用 |
| CN117085079A (zh) * | 2023-10-20 | 2023-11-21 | 妙越缇卡生物医药科技(山东)有限公司 | 植物乳杆菌sf-l38的发酵制品及其制备方法和应用 |
| CN117085079B (zh) * | 2023-10-20 | 2024-02-06 | 妙越缇卡生物医药科技(山东)有限公司 | 植物乳杆菌sf-l38的发酵制品及其制备方法和应用 |
| CN117646038A (zh) * | 2024-01-30 | 2024-03-05 | 妙越缇卡生物医药科技(山东)有限公司 | 美白淡斑的副干酪乳杆菌sf-l31发酵物及其应用 |
| CN117646038B (zh) * | 2024-01-30 | 2024-04-19 | 妙越缇卡生物医药科技(山东)有限公司 | 美白淡斑的副干酪乳杆菌sf-l31发酵物及其应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN116492284A (zh) | 美白抗菌的干酪乳杆菌sf-l-12发酵物及其应用 | |
| KR101457960B1 (ko) | 피부트러블 완화용 화장료 조성물 | |
| CN110934803B (zh) | 一种具有美白淡斑功能的植物发酵组合物 | |
| CN108815072B (zh) | 一种具有保湿舒缓功效的鲜活植物组合物发酵原浆及其制备方法和应用 | |
| KR20090002678A (ko) | 피부트러블 완화용 화장료 조성물 | |
| KR101456042B1 (ko) | 노화방지용 화장료 조성물 | |
| CN111632012B (zh) | 一种具有美白功能的复合益生菌发酵组合物及其应用 | |
| KR20080094456A (ko) | 미백용 화장료 조성물 | |
| KR20080094455A (ko) | 미백용 화장료 조성물 | |
| CN110638742A (zh) | 一种黑头导出组合物、一种收敛舒缓组合物及由两者组成的去黑头组合物 | |
| CN105168057A (zh) | 玄参沐浴露 | |
| CN101104006B (zh) | 一种冬虫夏草菌提取浓缩液的制备方法及其应用 | |
| CN113694142A (zh) | 一种利用益生菌发酵抗衰老中药组方的制备方法 | |
| CN115414290B (zh) | 一种具有保湿、抗氧化、抗炎功效的中药组合物及其制备和应用 | |
| CN110974775A (zh) | 一种含葛仙米的舒敏组合物及其制备方法和应用 | |
| CN114632055B (zh) | 一种美白舒缓的植物发酵物及其制备方法和应用 | |
| CN110538123A (zh) | 一种美白祛斑的中药复方提取液的制备工艺及其应用 | |
| CN108451888A (zh) | 一种改善人体皮肤表面微循环的微生物质护肤品及其制备方法 | |
| CN113413336A (zh) | 一种添加松茸发酵提取物的美白组合物及制备方法和应用 | |
| CN112386543A (zh) | 白梅花总黄酮提取物的制备方法及应用 | |
| CN110664700A (zh) | 组合物及其在制备防治痤疮的产品中的应用 | |
| CN111888293B (zh) | 一种含有玫瑰发酵液的保湿爽肤水及其制备方法 | |
| CN117085086B (zh) | 一种补气血发酵物及其制备工艺、应用 | |
| CN115245475B (zh) | 六君子发酵物用于制备兼具抗氧化、保湿及控油功效的中草药化妆品组成物的用途 | |
| CN114931537B (zh) | 一种延缓衰老的复合发酵物及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20230728 |