CN116490603A - 在培养皿中增强器官发生的类器官培养工程 - Google Patents

在培养皿中增强器官发生的类器官培养工程 Download PDF

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Publication number
CN116490603A
CN116490603A CN202180080345.0A CN202180080345A CN116490603A CN 116490603 A CN116490603 A CN 116490603A CN 202180080345 A CN202180080345 A CN 202180080345A CN 116490603 A CN116490603 A CN 116490603A
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China
Prior art keywords
culture
organoid
organoids
cells
intestinal
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Pending
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CN202180080345.0A
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English (en)
Chinese (zh)
Inventor
胡东恩
晟希·埃斯特尔·朴
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University of Pennsylvania Penn
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University of Pennsylvania Penn
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Publication of CN116490603A publication Critical patent/CN116490603A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180080345.0A 2020-12-04 2021-12-06 在培养皿中增强器官发生的类器官培养工程 Pending CN116490603A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063121684P 2020-12-04 2020-12-04
US63/121,684 2020-12-04
PCT/US2021/072762 WO2022120391A1 (en) 2020-12-04 2021-12-06 Engineering of organoid culture for enhanced organogenesis in a dish

Publications (1)

Publication Number Publication Date
CN116490603A true CN116490603A (zh) 2023-07-25

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CN202180080345.0A Pending CN116490603A (zh) 2020-12-04 2021-12-06 在培养皿中增强器官发生的类器官培养工程

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Country Link
US (1) US20240026261A1 (https=)
EP (1) EP4256027A4 (https=)
JP (1) JP2023553869A (https=)
CN (1) CN116490603A (https=)
AU (1) AU2021390584A1 (https=)
CA (1) CA3197769A1 (https=)
WO (1) WO2022120391A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117448276A (zh) * 2023-12-06 2024-01-26 复旦大学附属妇产科医院 一种宫颈癌血管化类器官的制备方法
WO2025087283A1 (zh) * 2023-10-23 2025-05-01 江苏艾玮得生物科技有限公司 血管化类器官球聚体模型的构建方法、血管化类器官球聚体模型及其应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120225651A (zh) * 2022-11-21 2025-06-27 康宁公司 具有保持结构的微板及其使用方法
WO2025041139A1 (en) * 2023-08-23 2025-02-27 Quris Technologies Ltd. Fluidic multi well systems and methods of using the same for culturing organoids
EP4613841A1 (en) * 2024-03-04 2025-09-10 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen Microwell for culturing an organoid and device for measuring electrical activity and/or stimulating cells in a three-dimensional network
WO2025202125A1 (en) * 2024-03-28 2025-10-02 F. Hoffmann-La Roche Ag Cell culturing device

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009146911A2 (en) * 2008-06-04 2009-12-10 Uwe Marx Organ-on-a-chip-device
TWI509067B (zh) * 2014-04-10 2015-11-21 Nat Univ Tsing Hua 體外細胞自動抓取共養平台系統
DE102016110328B3 (de) * 2016-06-03 2017-09-07 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Lochplatte
US11692160B2 (en) * 2016-11-10 2023-07-04 The Asan Foundation Microfluidic chip, three-dimensional channel structure, cell culture method using same, and activity evaluation method of bioactive substance using same
WO2018175478A2 (en) * 2017-03-21 2018-09-27 Massachusetts Institute Of Technology Modular organ microphysiological system with microbiome
DE102017209942B4 (de) * 2017-06-13 2019-05-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Vorrichtung und Verfahren für die Kultivierung von Zellen
KR102083083B1 (ko) * 2018-06-21 2020-02-28 연세대학교 산학협력단 고효율 오가노이드 배양 디바이스 및 배양 시스템
JP2020115781A (ja) * 2019-01-23 2020-08-06 ウシオ電機株式会社 細胞培養チップ
GB201901273D0 (en) * 2019-01-30 2019-03-20 Stemnovate Ltd Method and apparatus for real time monitoring of cell and tissue culture

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025087283A1 (zh) * 2023-10-23 2025-05-01 江苏艾玮得生物科技有限公司 血管化类器官球聚体模型的构建方法、血管化类器官球聚体模型及其应用
CN117448276A (zh) * 2023-12-06 2024-01-26 复旦大学附属妇产科医院 一种宫颈癌血管化类器官的制备方法

Also Published As

Publication number Publication date
JP2023553869A (ja) 2023-12-26
EP4256027A4 (en) 2026-03-18
EP4256027A1 (en) 2023-10-11
CA3197769A1 (en) 2022-06-09
US20240026261A1 (en) 2024-01-25
WO2022120391A1 (en) 2022-06-09
AU2021390584A1 (en) 2023-06-22

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