CN116425571A - Method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria - Google Patents
Method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria Download PDFInfo
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- CN116425571A CN116425571A CN202310426305.8A CN202310426305A CN116425571A CN 116425571 A CN116425571 A CN 116425571A CN 202310426305 A CN202310426305 A CN 202310426305A CN 116425571 A CN116425571 A CN 116425571A
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- biogas slurry
- fermentation
- rapid fermentation
- beneficial microorganism
- promoting rapid
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- 239000002002 slurry Substances 0.000 title claims abstract description 66
- 238000000855 fermentation Methods 0.000 title claims abstract description 41
- 230000004151 fermentation Effects 0.000 title claims abstract description 41
- 241000894006 Bacteria Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 24
- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 230000009286 beneficial effect Effects 0.000 title claims abstract description 19
- 230000001737 promoting effect Effects 0.000 title claims abstract description 17
- 239000002131 composite material Substances 0.000 title abstract description 10
- 210000003608 fece Anatomy 0.000 claims abstract description 27
- 239000010871 livestock manure Substances 0.000 claims abstract description 26
- 239000000126 substance Substances 0.000 claims abstract description 17
- 235000016709 nutrition Nutrition 0.000 claims abstract description 16
- 230000035764 nutrition Effects 0.000 claims abstract description 16
- 244000144977 poultry Species 0.000 claims abstract description 16
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 15
- 239000002689 soil Substances 0.000 claims abstract description 13
- 239000006041 probiotic Substances 0.000 claims abstract description 12
- 235000018291 probiotics Nutrition 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 229930000044 secondary metabolite Natural products 0.000 claims abstract description 6
- 230000008485 antagonism Effects 0.000 claims abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 12
- 150000007524 organic acids Chemical class 0.000 claims description 9
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 6
- 102000018997 Growth Hormone Human genes 0.000 claims description 6
- 108010051696 Growth Hormone Proteins 0.000 claims description 6
- 239000003242 anti bacterial agent Substances 0.000 claims description 6
- 229940088710 antibiotic agent Drugs 0.000 claims description 6
- 239000000122 growth hormone Substances 0.000 claims description 6
- 239000003617 indole-3-acetic acid Substances 0.000 claims description 6
- 230000008635 plant growth Effects 0.000 claims description 6
- 230000002401 inhibitory effect Effects 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- 241001655322 Streptomycetales Species 0.000 claims description 3
- 229930003779 Vitamin B12 Natural products 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 230000000975 bioactive effect Effects 0.000 claims description 3
- 235000005985 organic acids Nutrition 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 235000019156 vitamin B Nutrition 0.000 claims description 3
- 239000011720 vitamin B Substances 0.000 claims description 3
- 239000011715 vitamin B12 Substances 0.000 claims description 3
- 235000019163 vitamin B12 Nutrition 0.000 claims description 3
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 abstract description 4
- 108010028921 Lipopeptides Proteins 0.000 abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 244000144972 livestock Species 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000002440 industrial waste Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Fertilizers (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention provides a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, belonging to the field of biogas slurry fermentation. In particular to a method for accelerating biogas slurry fermentation by adding poultry manure. The method comprises the steps of utilizing antibacterial property and multiple nutrients in biogas slurry fermentation to enable good and bad microorganisms in soil to generate antagonism, adopting a comprehensive bacterial population to inhibit primary bacteria, carrying out aerobic rapid fermentation on the comprehensive bacterial population, rapidly decomposing organic substances in biogas slurry and biogas residues, generating lipopeptides in secondary metabolites thereof, providing nutrition for other probiotics in the soil, carrying out rapid fermentation on the comprehensive bacterial population (poultry manure biogas slurry), rapidly decomposing the organic substances in the biogas slurry and biogas residues, generating lipopeptides in the secondary metabolites thereof, providing nutrition for other probiotics in the soil, competing for nutrition by pathogenic bacteria, and forming inhibition.
Description
Technical Field
The invention provides a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, belonging to the field of biogas slurry fermentation. In particular to a method for accelerating biogas slurry fermentation by adding poultry manure.
Background
At present, because livestock and poultry manure is stacked and is inappropriately managed, a large amount of pollutants enter a water body, the livestock and poultry manure enters the water body, the water body loss rate is up to 25% -30%, the COD emission is close to the total industrial wastewater COD emission, the N, P loss is larger than the chemical fertilizer loss, and the water body is one of the main reasons of rural non-point source pollution, if the livestock and poultry manure cannot be timely treated, the surrounding environment and the water source are seriously polluted, and the ecological environment is seriously influenced, so that the manure produced by the livestock and poultry industry becomes one of the important factors restricting the deep development of a breeding enterprise, and although livestock and poultry manure, agricultural wastes, industrial wastes and the like are partially utilized to prepare organic fertilizers, a certain amount of harmful substances such as heavy metals, pesticides, feed additives and antibiotics can exist, and the harmful substances can be brought into soil and the environment along with the harmful substances, and in addition, although the organic fertilizers have more component types, each nutrient content is relatively less, the utilization rate is low, and the bacteria are more.
Disclosure of Invention
The invention provides a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, and provides a fermentation method for performing rapid fermentation by adding poultry manure into the biogas slurry. Simple structure and convenient use.
The invention relates to a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, which is realized by utilizing the beneficial microorganism composite bacteria, wherein the method utilizes the antibacterial property and multiple nutrients in the fermentation of the biogas slurry to enable good and bad microorganisms in soil to generate antagonism, and adopts a comprehensive bacterial population to inhibit primary bacteria, the comprehensive bacterial population carries out aerobic rapid fermentation to rapidly decompose organic substances in biogas residues of the biogas slurry, and lipid peptides can be generated in secondary metabolites of the organic substances to provide nutrition for other probiotics groups in soil;
the fermentation period of the comprehensive strain group is within 5 days;
the comprehensive strain group comprises three bacillus, two streptomycete groups and other probiotics groups;
the fermented biogas slurry contains various bioactive substances including but not limited to organic acids, trace elements, plant growth hormone, B vitamins and antibiotics;
the organic acid is mainly amino acid and indoleacetic acid;
butyric acid, indoleacetic acid in the organic acid and penicillin and vitamin B12 in the plant growth hormone are used for inhibiting pathogenic bacteria;
the antibiotics in the fermented biogas slurry have direct inhibition effect on plant diseases and insect pests of crops;
the fermented biogas slurry also contains ammonia and salt, wherein the ammonia and the salt are used for inhibiting plant diseases and insect pests of crops;
the probiotics colony of the fermented biogas slurry can compete with pathogenic bacteria for nutrition in competition with the pathogenic bacteria, so that the nutrition around the pathogenic bacteria can be rapidly absorbed and depleted, and the prevention and treatment effect is achieved;
further, poultry manure biogas slurry is added into the fermentation biogas slurry;
further, the poultry manure biogas slurry comprises one or more of chicken manure biogas slurry, pig manure biogas slurry and cow manure biogas slurry;
the invention relates to a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, which adopts comprehensive bacterial population (poultry manure biogas slurry) to perform rapid fermentation, so as to rapidly decompose organic substances in biogas slurry biogas residue, and lipid peptide can be generated in secondary metabolites of the organic substances, thereby providing nutrition for other probiotics in soil, competing nutrition with pathogenic bacteria and forming inhibition effect.
Description of the embodiments
The invention relates to a method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria, which is realized by utilizing the beneficial microorganism composite bacteria, wherein the method utilizes the antibacterial property and multiple nutrients in the fermentation of the biogas slurry to enable good and bad microorganisms in soil to generate antagonism, and adopts a comprehensive bacterial population to inhibit primary bacteria, the comprehensive bacterial population carries out aerobic rapid fermentation to rapidly decompose organic substances in biogas residues of the biogas slurry, and lipid peptides can be generated in secondary metabolites of the organic substances to provide nutrition for other probiotics groups in soil;
the fermentation period of the comprehensive strain group is within 5 days;
the comprehensive strain group comprises three bacillus, two streptomycete groups and other probiotics groups;
the fermented biogas slurry contains various bioactive substances including but not limited to organic acids, trace elements, plant growth hormone, B vitamins and antibiotics;
the organic acid is mainly amino acid and indoleacetic acid;
butyric acid, indoleacetic acid in the organic acid and penicillin and vitamin B12 in the plant growth hormone are used for inhibiting pathogenic bacteria;
the antibiotics in the fermented biogas slurry have direct inhibition effect on plant diseases and insect pests of crops;
the fermented biogas slurry also contains ammonia and salt, wherein the ammonia and the salt are used for inhibiting plant diseases and insect pests of crops;
the probiotics colony of the fermented biogas slurry can compete with pathogenic bacteria for nutrition in competition with the pathogenic bacteria, so that the nutrition around the pathogenic bacteria can be rapidly absorbed and depleted, and the prevention and treatment effect is achieved;
further, poultry manure biogas slurry is added into the fermentation biogas slurry;
further, the poultry manure biogas slurry comprises one or more of chicken manure biogas slurry, pig manure biogas slurry and cow manure biogas slurry;
further, the content of metal ions in the fermented biogas slurry is as follows:
the functions of biogas slurry fermentation are the results of multiple action mechanisms, including competition for nutrition and space with pathogenic bacteria, promotion of decomposition of macromolecules in soil and effective absorption of nutrition, improvement of soil properties, promotion of crop growth and disease resistance, and the like. Successful antagonism is achieved with a number of factors in combination. The probiotics in the fermentation liquor can compete with pathogenic bacteria for nutrition in competition with the pathogenic bacteria, so that the nutrition around the pathogenic bacteria can be rapidly absorbed completely, and the prevention and treatment effect is achieved.
The invention and its embodiments have been described above with no limitation, but the examples shown are only one of the embodiments of the invention, and the actual structure is not limited thereto. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present invention.
Claims (9)
1. A method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria is characterized by comprising the following steps: the antibacterial property and multiple nutrients in biogas slurry fermentation are utilized to enable good and bad microorganisms in soil to generate antagonism, and a comprehensive bacterial population is adopted to inhibit primary bacteria, wherein the comprehensive bacterial population is subjected to aerobic rapid fermentation to rapidly decompose organic substances in biogas slurry and biogas residues, and secondary metabolites of the organic substances can generate lipopeptid to provide nutrition for other probiotics groups in soil.
2. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 1, which is characterized in that: the fermentation period of the comprehensive strain group is within 5 days.
3. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 1, which is characterized in that: the comprehensive strain group comprises three bacillus, two streptomycete groups and other probiotics groups.
4. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 1, which is characterized in that: the fermented biogas slurry contains various bioactive substances including but not limited to organic acids, trace elements, plant growth hormone, B vitamins and antibiotics.
5. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 4, which is characterized in that: the organic acid is mainly amino acid and indoleacetic acid.
6. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 4, which is characterized in that: butyric acid, indoleacetic acid in the organic acid and penicillin and vitamin B12 in the plant growth hormone are used for inhibiting pathogenic bacteria.
7. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 1, which is characterized in that: poultry excrement biogas slurry is added into the fermentation biogas slurry.
8. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 7, wherein the method comprises the following steps: the poultry manure biogas slurry comprises one or more of chicken manure biogas slurry, pig manure biogas slurry and cow manure biogas slurry.
9. The method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism complex bacteria according to claim 8, which is characterized in that: the chicken manure biogas slurry comprises the following components: n:0.54%, P 2 O 5 ：0.25%、K 2 O:0.52%, total Nutrient (NPK): 1.33%;
the pig manure biogas slurry comprises the following components: n:0.41%, P 2 O 5 ：0.2%、K 2 O:0.31%, total Nutrient (NPK): 1.22%;
the cow dung biogas slurry comprises the following components: n:0.31%, P 2 O 5 ：0.16%、K 2 O:0.32%, total Nutrient (NPK): 0.79%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310426305.8A CN116425571A (en) | 2023-04-20 | 2023-04-20 | Method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310426305.8A CN116425571A (en) | 2023-04-20 | 2023-04-20 | Method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria |
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| Publication Number | Publication Date |
|---|---|
| CN116425571A true CN116425571A (en) | 2023-07-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310426305.8A Pending CN116425571A (en) | 2023-04-20 | 2023-04-20 | Method for promoting rapid fermentation of biogas slurry by utilizing beneficial microorganism composite bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116425571A (en) |
-
2023
- 2023-04-20 CN CN202310426305.8A patent/CN116425571A/en active Pending
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