CN116370369B - Natural plant extract composition with effects of relieving, repairing and resisting allergy and application thereof - Google Patents
Natural plant extract composition with effects of relieving, repairing and resisting allergy and application thereof Download PDFInfo
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- CN116370369B CN116370369B CN202310080939.2A CN202310080939A CN116370369B CN 116370369 B CN116370369 B CN 116370369B CN 202310080939 A CN202310080939 A CN 202310080939A CN 116370369 B CN116370369 B CN 116370369B
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/04—Antipruritics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
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- Dermatology (AREA)
- Birds (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to a natural plant extract composition with effects of relieving, repairing and resisting allergy and application thereof. The natural plant extract composition comprises cynanchum atratum extract, bletilla root extract, kuh-seng root extract, citrus fruit extract, stephania tetrandra root extract and andrographis paniculata extract. In another aspect, the present invention also relates to a composition of the main active ingredient monomers in the above natural plant extract composition, comprising cynaroside, bletilla glycoside, matrine, hesperidin, tetrandrine and andrographolide. The natural active ingredient monomer composition has lower dosage than the corresponding natural plant extract composition and has stronger effects of relieving, repairing and resisting sensitization. Experimental results show that various active ingredients in the composition can be mutually matched, and the composition has a synergistic effect, can jointly control skin inflammation, improve skin allergy, itching and stinging, and enables skin to be in a more stable state.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a natural plant extract composition with effects of relieving, repairing and resisting allergy and application thereof.
Background
Sensitive skin affects the quality of life of people to a large extent. Sensitive skin usually has skin allergy symptoms and discomfort such as redness, itching, dryness, stinging, burning, tightness and the like, and although a specific allergen is difficult to find, the sensitive skin has common points of "skin barrier damage", namely that the skin surface loses normal protective function and loss of skin moisture occurs, so that dryness, desquamation or itching of the skin occurs. Meanwhile, allergens such as germs, pollen, chemical substances and the like on the surface of the skin easily pass through damaged skin barriers and enter the interior of the skin, so that skin inflammatory reaction is further initiated.
At present, cosmetics for treating or relieving skin allergy mainly adopt the thought that antiallergic components are added into the cosmetics, even medicines such as glucocorticoid are adopted for treatment, but repair of skin barrier functions is often neglected, so that the products can lead to thinning of epidermis and dermis after long-term use, the skin barrier functions are damaged, and symptoms such as skin atrophy, telangiectasia and the like appear. Or, most of cosmetics with effects of relieving, repairing and resisting sensitization are common in the market to improve the moisturizing effect so as to achieve the repairing effect. But only the skin irritation can be relieved briefly by moisturizing, and the problems that fragile sensitive skin is easy to feel stinging and itching, and is easy to reddish and has red blood streaks can not be fundamentally solved.
Aiming at the problem that the effect of the skin care product for treating or relieving skin allergy is not satisfactory at present, the inventor adopts a modern research method to develop and utilize natural plant extracts, and combines a large number of experimental screening to develop a natural plant extract composition with the efficacy of relieving, repairing and resisting allergy.
Disclosure of Invention
In order to solve the technical problems, the invention provides a natural plant extract composition with effects of relieving, repairing and resisting sensitization and application thereof. The invention realizes the remarkable effects of diminishing inflammation, preserving moisture, relieving and repairing through the mutual synergistic cooperation of various plant extracts in the composition, thereby improving the sensitive skin state, and the composition can not generate drug resistance after long-term use, has no side effect, and is very safe and reliable.
Specifically, the invention is realized through the following technical schemes:
in a first aspect, the present invention provides a natural plant extract composition having effects of soothing, repairing and anti-allergic, the composition comprising, in parts by weight, 1-5 parts of cynanchum atratum extract, 1-5 parts of bletilla tuber extract, 1-5 parts of kuh-seng root extract, 0.1-2 parts of citrus fruit extract, 0.1-2 parts of tetrandra root extract and 0.1-2 parts of andrographis paniculata extract.
Alternatively, in the above natural plant extract composition, the composition comprises 2 to 3 parts by weight of cynanchum atratum extract, 1.5 to 2.5 parts by weight of bletilla striata root extract, 1 to 2 parts by weight of kuh-seng root extract, 0.5 to 1 part by weight of citrus fruit extract, 0.5 to 1 part by weight of stephania tetrandra root extract and 0.5 to 1 part by weight of andrographis paniculata extract.
Alternatively, in the above natural plant extract composition, the composition comprises, in parts by weight, 3 parts of cynanchum atratum extract, 2 parts of bletilla striata root extract, 1.5 parts of kuh-seng root extract, 0.5 part of citrus fruit extract, 0.5 part of stephania tetrandra extract and 0.5 part of andrographis paniculata extract.
Alternatively, in the above natural plant extract composition, the composition is prepared from 3 parts by weight of cynanchum atratum extract, 2 parts by weight of bletilla striata root extract, 1.5 parts by weight of kuh-seng root extract, 0.5 part by weight of citrus fruit extract, 0.5 part by weight of tetrandra root extract and 0.5 part by weight of andrographis paniculata extract.
Alternatively, in the above natural plant extract composition, the cynanchum atratum extract is cynanchum atratum glycoside, the bletilla striata root extract is bletilla striata glycoside, the sophocarpidine root extract is matrine, the citrus fruit extract is hesperidin, the tetrandrine extract is tetrandrine, and the andrographis paniculata extract is andrographolide.
Alternatively, in the above natural plant extract composition, the composition comprises, in parts by weight, 1 to 5 parts of cynanchum atratum glycoside, 1 to 5 parts of bletilla glycoside, 1 to 5 parts of matrine, 0.1 to 2 parts of hesperidin, 0.1 to 2 parts of tetrandrine and 0.1 to 2 parts of andrographolide.
Alternatively, in the above natural plant extract composition, the composition comprises, in parts by weight, 2 to 3 parts of cynanchum atratum glycoside, 1.5 to 2.5 parts of bletilla striata glycoside, 1 to 2 parts of matrine, 0.5 to 1 part of hesperidin, 0.5 to 1 part of tetrandrine and 0.5 to 1 part of andrographolide.
Alternatively, in the above natural plant extract composition, the composition comprises 3 parts by weight of cynaroside, 2 parts by weight of bletilla glycoside, 1.5 parts by weight of matrine, 0.5 part by weight of hesperidin, 0.5 part by weight of tetrandrine and 0.5 part by weight of andrographolide.
Alternatively, in the above natural plant extract composition, the composition is prepared from 3 parts by weight of cynanchum atratum glycoside, 2 parts by weight of bletilla glycoside, 1.5 parts by weight of matrine, 0.5 part by weight of hesperidin, 0.5 part by weight of tetrandrine and 0.5 part by weight of andrographolide.
In a second aspect, the present invention provides a method for preparing the natural plant extract composition of the first aspect, the method comprising the steps of: and uniformly mixing the raw materials to obtain the natural plant extract composition.
In a third aspect, the present invention provides the use of the natural plant extract composition of the first aspect above for the preparation of a cosmetic having soothing, repairing and anti-allergic effects.
Alternatively, in the above-mentioned use, the cosmetic is a toner, an emulsion, an essence, a cream or a mask.
Alternatively, in the above-mentioned use, the composition is added to the cosmetic in an amount of 0.05% to 10% by weight.
Preferably, when the active ingredient is a natural plant extract, the composition is added to the cosmetic in an amount of 5% to 10% by weight.
It is also preferable that the composition is added to the cosmetic in an amount of 0.05% to 0.1% by weight when the active ingredient is a natural active ingredient monomer.
It is understood that within the scope of the present invention, the above-described technical features of the present invention and technical features specifically described below (e.g., in the examples) may be combined with each other to constitute new or preferred technical solutions. Is limited to a space and will not be described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
(1) The invention breaks through the traditional antiallergic therapy concept, adopts a modern research method to develop and utilize natural plant extracts, combines a large number of experimental screening, and develops a natural plant extract composition with the efficacy of relieving, repairing and antiallergic.
(2) The composition of the invention can not generate drug resistance after long-term use, has no side effect, and is very safe and reliable.
(3) Experimental results show that various active ingredients in the composition can be mutually matched, and the composition has a synergistic effect, can jointly control skin inflammation, improve skin allergy, itching and stinging, and enables skin to be in a more stable state.
Detailed Description
In the intensive study of the efficacy of relieving, repairing and resisting allergy of natural plant extracts, the inventor unexpectedly obtains a safe and efficient natural plant extract composition with obvious synergistic effect among various active ingredients through a large number of screening. The present invention has been completed on the basis of this finding.
In order to better understand the present invention, the effects of the main raw materials and auxiliary materials in the composition of the present invention are further explained below.
The "cynanchum atratum (CYNANCHUM ATRATUM) extract" is extracted from the rhizome of cynanchum atratum, and is found by Korean medical research to be a purely natural "super repair factor". The cynanchum atratum extract has strong anti-inflammatory and antiallergic effects, and can relieve inflammatory skin, repair damaged cells and prevent skin aging. The cynanchum atratum extract contains a plurality of natural product active ingredient monomers such as cynanchum atratum glycoside, C21 steroid saponin, flavone and the like.
The bletilla STRIATA (Bletila STRIATA) root extract has the main effects of skin conditioning agent, whitening and freckle removing in cosmetics and skin care products, is safer, has no influence on pregnant women generally, and has no acne-causing property. The bletilla striata extract has an inhibiting effect on 5 a-reductase, which shows that the extract has a good preventing and treating effect on alopecia caused by high androgen, and can be used for preparing hair-growing and acne products; the bletilla striata extract is also an antioxidant, a skin whitening agent and an anti-inflammatory agent with obvious effects. The rhizoma bletilla root extract contains active ingredient monomers of natural products such as rhizoma bletilla glycoside and polysaccharide.
The radix sophorae flavescentis (SOPHORA ANGUSTFOLIA) root extract has the main functions of an antioxidant, a skin conditioner and an anti-inflammatory agent in cosmetics and skin care products, is safe, has no influence on pregnant women generally, and has no acne-causing property. The radix Sophorae Flavescentis extract mainly comes from root of radix Sophorae Flavescentis, and its effective component is matrine, which is a traditional herbal medicine. Can be used for treating eczema and dermatitis, and has antibacterial and antiinflammatory effects; has effects in promoting collagen production, promoting fibroblast proliferation, and resisting skin aging; meanwhile, the product has the effect of moisturizing.
"citrus (CIITRUS RETICULATA) fruit extract" can be used as an antioxidant, anti-inflammatory and antibacterial agent. The citrus fruit extract has strong broad-spectrum antibacterial property, has good inhibition effect on acne-causing bacteria, and can be used in related products for expelling parasites and removing mites; has effects of scavenging various free radicals, is an ideal antioxidant, and has antiaging effect in combination with proliferation of epidermal fibroblast. The main active ingredient in the citrus fruit extract is hesperidin.
The radix stephaniae tetrandrae (STEPHANIA TETRANDRA) extract has broad-spectrum anti-inflammatory effect, has different degrees of inhibition on all links of inflammatory reaction, and can be used as anti-inflammatory agent of cosmetics; animal experiments show that the radix stephaniae tetrandrae extract has good astringing effect on pores, and can be used for skin tightening cosmetics. The radix Stephaniae Tetrandrae extract contains various isoquinoline alkaloids, such as radix Stephaniae Tetrandrae alkali, fangchinoline, and rotiferine.
The herba Andrographitis (ANDROGRAPHIS PANICULATA) extract can be used as skin conditioner and astringent, and has effects of clearing heat, removing toxic substances, and relieving swelling. It is used in cosmetics to make skin smooth and shiny. The main active ingredient in the herba Andrographitis extract is andrographolide.
The cynanchum atratum (CYNANCHUM ATRATUM) extract, the bletilla STRIATA (bletilla STRIATA strata) root extract, the radix sophorae flavescentis (SOPHORA ANGUSTFOLIA) root extract, the citrus (CIITRUS RETICULATA) fruit extract, the stephania tetrandra (STEPHANIA TETRANDRA) extract and the andrographis paniculata (ANDROGRAPHIS PANICULATA) extract used in the cosmetic composition can be common cosmetic raw materials obtained by commercial purchase, and can also be prepared by a conventional method in the field of natural plant extracts. In the natural plant extract composition, cynanchum atratum glycoside derived from cynanchum atratum extract, bletilla glycoside derived from bletilla root extract, matrine derived from sophorae flavescentis root extract, hesperidin derived from citrus fruit extract, tetrandrine derived from tetrandrine extract and andrographolide derived from andrographis paniculata extract can be obtained by commercial purchase or extraction, separation and purification by a conventional method in the field of natural plant extraction. Thus, one of ordinary skill in the cosmetic arts can choose to add natural plant extracts or natural active ingredient monomers of varying cost and effect to the cosmetic composition to provide more options for different consumer populations at different prices.
The invention will be further illustrated with reference to specific examples. It should be understood that the detailed description and specific examples are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The specific techniques or conditions are not identified in the examples and are described in the literature in this field or are carried out in accordance with the product specifications. The reagents or equipment used were conventional products available for purchase through regular channels, with no manufacturer noted.
The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, are all commercially available products.
Preparation examples:
1. a natural plant extract composition:
example 1.1:
a natural plant extract composition with effects of relieving, repairing and resisting sensitization is prepared from 3 parts by weight of cynanchum atratum extract, 2 parts by weight of bletilla striata root extract, 1.5 parts by weight of radix sophorae flavescentis extract, 0.5 part by weight of citrus fruit extract, 0.5 part by weight of radix stephaniae tetrandrae extract and 0.5 part by weight of herba andrographitis extract.
The preparation method of the natural plant extract composition comprises the following steps: and uniformly mixing the raw materials to obtain the natural plant extract composition.
Comparative example 1.1:
a natural plant extract composition is prepared from 1 part by weight of cynanchum atratum extract, 1 part by weight of bletilla tuber extract, 1.5 parts by weight of radix sophorae flavescentis extract, 0.5 part by weight of citrus fruit extract, 2.5 parts by weight of radix stephaniae tetrandrae extract and 1.5 parts by weight of herba andrographitis extract.
The preparation method is the same as in example 1.1.
Comparative example 1.2:
a natural plant extract composition is prepared from 3 parts by weight of cynanchum atratum extract, 2 parts by weight of bletilla tuber extract, 1.5 parts by weight of radix sophorae flavescentis extract and 0.5 part by weight of citrus fruit extract.
The preparation method is the same as in example 1.1.
Comparative example 1.3:
a natural plant extract composition is prepared from 0.5 part of radix stephaniae tetrandrae extract and 0.5 part of herba andrographitis extract in parts by weight.
The preparation method is the same as in example 1.1.
Comparative example 1.4:
a natural plant extract composition with effects of relieving, repairing and resisting sensitization is prepared from 3 parts by weight of centella asiatica extract, 2 parts by weight of bletilla striata root extract, 1.5 parts by weight of paeonia lactiflora root extract, 0.5 part by weight of citrus fruit extract, 0.5 part by weight of tetrandra root extract and 0.5 part by weight of andrographis paniculata extract.
The preparation method is the same as in example 1.1.
The natural plant extract composition prepared in example 1.1, comparative example 1.1 and comparative example 1.4 was taken in 8 parts by weight, and a general cream base was taken in 92 parts by weight, to prepare a cream containing 8% of the natural plant extract composition. In the same manner, comparative examples 1.2 and 1.3 were prepared as a face cream containing 7% and 1% of the natural plant extract composition, respectively, for use in the subsequent experiments.
2. Natural active ingredient monomer composition:
example 2.1:
a natural active ingredient monomer composition with effects of relieving, repairing and resisting allergy is prepared from 3 parts of cynanchum atratum glycoside, 2 parts of bletilla glycoside, 1.5 parts of matrine, 0.5 part of hesperidin, 0.5 part of tetrandrine and 0.5 part of andrographolide in parts by weight.
The preparation method of the natural active ingredient monomer composition comprises the following steps: and uniformly mixing the raw materials to obtain the natural active ingredient monomer composition.
Comparative example 2.1:
a natural active ingredient monomer composition is prepared from 1 part by weight of cynanchum atratum glycoside, 1 part by weight of bletilla glycoside, 1.5 parts by weight of matrine, 0.5 part by weight of hesperidin, 2.5 parts by weight of tetrandrine and 1.5 parts by weight of andrographolide.
The preparation method is the same as in example 2.1.
Comparative example 2.2:
a natural active ingredient monomer composition is prepared from 3 parts of cynanchum atratum glycoside, 2 parts of bletilla glycoside, 1.5 parts of matrine and 0.5 part of hesperidin in parts by weight.
The preparation method is the same as in example 2.1.
Comparative example 2.3:
a natural active ingredient monomer composition is prepared from 0.5 part of tetrandrine and 0.5 part of andrographolide in parts by weight.
The preparation method is the same as in example 2.1.
Comparative example 2.4:
a natural active ingredient monomer composition is prepared from 3 parts by weight of asiaticoside, 2 parts by weight of bletilla glycoside, 1.5 parts by weight of white paeony root glycoside, 0.5 part by weight of hesperidin, 0.5 part by weight of tetrandrine and 0.5 part by weight of andrographolide.
The preparation method is the same as in example 2.1.
The natural plant extract composition prepared in example 2.1, comparative example 2.1 and comparative example 2.4 was taken in parts by weight to prepare 0.08 parts of a normal cream base 99.92 parts, and a cream containing 0.08% of the natural plant extract composition was prepared. In the same manner, comparative examples 2.2 and 2.3 were prepared as a cream containing 0.07% and 0.01% of the natural plant extract composition, respectively, for use in the subsequent experiments.
Effect examples:
effect example 1: acute skin irritation test of the Natural plant extract composition of the invention
Experimental method of acute skin irritation experiment referring to skin irritation/corrosiveness experiment in chapter six of cosmetic safety technical Specification, the hair removal area is prepared for shaving New Zealand rabbits before the experiment, and the skin is confirmed to be intact. The creams prepared from the compositions of examples 1.1 to 2.1 and comparative examples 1.1 to 1.4 and 2.1 to 2.4 were directly used as test substances, respectively and uniformly applied to the corresponding dehaired areas, covered with patches, and then fixed with non-irritating adhesive tapes and bandages. The other dehaired area patch coverage was kept as a control. After application for 4 hours, residual test substances are removed by warm water, skin reactions of the test parts are observed at 1, 24, 48 and 72 hours after removal, scores of each observation time point are recorded according to skin irritation reaction scores of table 1, the average value of the integral of the test animals is calculated, and the skin irritation intensity is judged in a grading manner according to the skin irritation intensity of table 2 according to the highest integral average value of each observation time point of 24, 48 and 72 hours. The test results are shown in Table 3.
Table 1: skin irritation response score
Table 2: skin irritation intensity grading
| Integral mean value | Strength of |
| 0-<0.5 | No irritation |
| 0.5-<2.0 | Light irritation |
| 2.0-<6.0 | Mid-irritation |
| 6.0-8.0 | Strong irritation |
Table 3: acute skin irritation test results of the inventive compositions
Effect example 2: testing of the anti-inflammatory Effect of the Natural plant extract composition of the present invention
The effect of the compositions of examples 1.1-2.1 and comparative examples 1.1-1.4 and 2.1-2.4 on the histamine release and pro-inflammatory cytokine expression of KU812 cells was examined as follows:
preparing and treating a tested sample: 0.8mg of the compositions of example 1.1, comparative example 1.1 and comparative example 1.4, respectively, were dissolved in 10mL of DMSO and diluted to a plant extract composition concentration of 0.04mg/mL in IMDM medium with 10% fetal bovine serum. Experimental samples of the compositions of comparative example 1.2 and comparative example 1.3 were prepared in the same manner, at concentrations of 0.035mg/mL and 0.005mg/mL, respectively. 0.008mg of the compositions of example 2.1, comparative example 2.1 and comparative example 2.4 were dissolved with 10mL of DMSO, respectively, and diluted to a plant extract composition concentration of 0.0004mg/mL with an IMDM medium of 10% fetal bovine serum. Experimental samples of the compositions of comparative example 2.2 and comparative example 2.3 were prepared in the same manner, at concentrations of 0.00035mg/mL and 0.00005mg/mL, respectively.
Cell culture and model establishment: by using a container containingThe cell density was diluted to 2.2X10 in IMDM medium with 10% fetal bovine serum 6 Each mL was then plated in 96-well plates at an inoculum size of 180. Mu.L per well.
Grouping and processing: the method comprises the steps of dividing the experimental group, the model control group, the blank control group and setting 3 parallel groups in each group; wherein 20. Mu.L of the test sample (examples 1.1-2.1 and comparative examples 1.1-1.4 and 2.1-2.4) was added to each of the experimental group and the model control group, respectively, 20. Mu.L of IMDM culture solution containing 10% fetal bovine serum was added to each of the blank control group and the model control group, and the mixture was placed at 37℃and 5% CO 2 After incubation in the incubator for 2h, 40nM crotyl alcohol-12-tetradecanoate-13-acetate (PMA) 5. Mu.L and 1. Mu.M calcium ionophore (A23187) 5. Mu.L were added to the experimental and model control groups, respectively; the blank group was added with 10. Mu.L of DMSO.
And (3) index detection: after 2h, the cells were centrifuged, and the cell supernatant was collected, and the content of histamine and IL-1. Alpha. In the supernatant was determined according to ELISA kit instructions, and the content of histamine and IL-1. Alpha. In the empty white control group was significantly lower than that in the model control group, indicating successful modeling.
Histamine release inhibition (%) = (model group histamine content-experimental group histamine content)/model group histamine content x 100%;
inhibition rate of IL-1α release (%) = (model group IL-1α content-experimental group IL-1α content)/model group IL-1α content; the results of the histamine release inhibition and IL-1. Alpha. Release inhibition of each group are shown in Table 4.
Table 4: effect of the Natural plant extract composition of the present invention on histamine release and IL-1α release from KU812 cells
The data in Table 4 shows that the plant extract compositions provided in examples 1.1 and 2.1 of the present invention significantly inhibit the release of histamine and IL-1α from KU812 cells, indicating that the compositions are effective in inhibiting inflammation and histamine release, thereby improving the condition of allergic skin.
Although the concentration of the natural active ingredient monomer of example 2.1 is one percent of the natural plant extract composition of example 1.1, the effect of inhibiting histamine release and IL-1 alpha release is slightly stronger than that of example 1.1. The experimental results of comparative examples 1.1 to 1.4 and comparative examples 2.1 to 2.4 also had similar tendency to change as in the above examples.
In addition, it was found from the analysis of the experimental results of each comparative example group that the ratio and kind of the amount of each component in the composition had a significant effect on the effect of the composition in inhibiting the release of histamine and IL-1. Alpha. From KU812 cells. For example, when the amounts of cynanchum atratum extract (or cynanchum atratum glycoside) and bletilla striata extract (or bletilla striata glycoside) in the composition are reduced, and the amounts of tetrandrine extract (or tetrandrine) and andrographis paniculata extract (or andrographolide) are correspondingly increased, the effect of the composition of the present invention on inhibiting histamine release and IL-1 alpha release is reduced. Also, when part of the active ingredient is replaced with an ingredient having the same or similar efficacy, the effect of the composition of the present invention in inhibiting histamine release and IL-1. Alpha. Release is also reduced. In addition, it has been found that the various active ingredients in the compositions of the present invention have a significant synergistic effect, which is significantly better than the combination of the first four components and the combination of the second two components when the six active ingredients in the compositions of the present invention are used in combination.
Effect example 3: skin barrier function test for repairing compositions of the present invention
1. Preparation before testing:
(1) Selection of the subjects:
66 subjects, 50 for men and women, between 18-50 years of age, were randomized into 11 groups (6 subjects per group): a cream base, examples 1.1-2.1 and comparative examples 1.1-1.4 and 2.1-2.4.
(2) Other experimental materials:
3% aqueous SDS (sodium dodecyl sulfate);
hyposensitization external adhesive tape (U.S. 3M company)
Mexameter MXI8 red pigment tester (Germany Courage-Khazaka Electronic company)
2. The experimental method comprises the following steps:
the amount of skin erythema was measured with a Mexameter MXl8 red pigment tester at a predetermined test area of the forearm of the subject, and the amount of skin erythema at this time was set to 0 (baseline value). 0.02mL of 3% SDS solution was accurately measured by a pipette, placed in the center of each plaque laboratory, and then applied to the forearm preset area of the volunteer. After 24 hours of application, the plaque tester was removed. After 4 hours, the amount of erythema on the skin was measured, and the amount of erythema at this time was 100%. The same amount of the cream and base of the compositions of the present invention of examples 1.1 to 2.1 and comparative examples 1.1 to 1.4 and 2.1 to 2.4, prepared in the experimental area of the forearm of the subject, respectively, was then applied on days 1 to 3 after the SDS treatment, and the amount of skin erythema was measured 4 hours after each application.
3. Experimental results:
the calculated and consolidated average test results are shown in table 5.
Table 5: test results of the effects of the Natural plant extract composition of the invention on the skin erythema
From the results in table 5, it can be seen that the effect of the cream-based group of subjects on the relief of experimental skin erythema was not ideal during the study.
The data in table 5 shows that the plant extract compositions provided in examples 1.1 and 2.1 of the present invention have a significant alleviating effect on experimental skin erythema in subjects. Although the concentration of the natural active ingredient monomer of example 2.1 is one percent of the natural plant extract composition of example 1.1, its effect of relieving experimental skin erythema is still slightly stronger than that of example 1.1. The experimental results of comparative examples 1.1 to 1.4 and comparative examples 2.1 to 2.4 also had similar tendency to change as in the above examples.
In addition, the experimental results of each comparative example group are analyzed, and the dosage proportion and the type of each component in the composition have obvious influence on the effect of the composition on relieving the erythema of the skin of the subjects. For example, when the amounts of cynanchum atratum extract (or cynanchum atratum glycoside) and bletilla striata extract (or bletilla striata glycoside) in the composition are reduced, and the amounts of tetrandrine extract (or tetrandrine) and andrographis paniculata extract (or andrographolide) are correspondingly increased, the effect of the composition for relieving the erythema on the skin of the subject is reduced. Also, when part of the active ingredient is replaced with an ingredient having the same or similar efficacy, the effect of the composition of the present invention on alleviating experimental erythema is also reduced. In addition, it has been found that the various active ingredients in the compositions of the present invention have a significant synergistic effect, which is significantly better than the combination of the first four components and the combination of the second two components when the six active ingredients in the compositions of the present invention are used in combination.
In summary, the invention breaks the idea of the traditional formula of the soothing, repairing and anti-allergic cosmetics, combines the functions of soothing, anti-inflammatory and anti-irritation with repairing the skin barrier, and the natural plant extract composition comprises various natural active ingredient extracts, has strong synergistic effect of soothing, anti-inflammatory, anti-irritation, repairing and anti-allergic effects, and is mild and non-irritating to the skin.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (8)
1. A natural plant extract composition with effects of relieving, repairing and resisting sensitization is characterized in that: the composition comprises, by weight, 1-5 parts of cynanchum atratum extract, 1-5 parts of bletilla tuber extract, 1-5 parts of radix sophorae flavescentis extract, 0.1-2 parts of citrus fruit extract, 0.1-2 parts of stephania tetrandra extract and 0.1-2 parts of andrographis paniculata extract.
2. The natural plant extract composition according to claim 1, wherein: the composition comprises, by weight, 2-3 parts of cynanchum atratum extract, 1.5-2.5 parts of bletilla tuber extract, 1-2 parts of radix sophorae flavescentis extract, 0.5-1 part of citrus fruit extract, 0.5-1 part of stephania tetrandra extract and 0.5-1 part of andrographis paniculata extract.
3. The natural plant extract composition according to claim 2, wherein: the composition comprises, by weight, 3 parts of cynanchum atratum extract, 2 parts of bletilla tuber extract, 1.5 parts of kuh-seng root extract, 0.5 part of citrus fruit extract, 0.5 part of stephania tetrandra extract and 0.5 part of andrographis paniculata extract.
4. The natural plant extract composition according to claim 1, wherein: the cynanchum atratum extract is cynanchum atratum glycoside, the bletilla striata root extract is bletilla striata glycoside, the kuh-seng root extract is matrine, the citrus fruit extract is hesperidin, the tetrandrine extract is tetrandrine, and the andrographis paniculata extract is andrographolide.
5. A method for preparing a natural plant extract composition as claimed in any one of claims 1 to 4, characterized in that: the preparation method comprises the following steps: and uniformly mixing the raw materials to obtain the natural plant extract composition.
6. Use of the natural plant extract composition as claimed in any one of claims 1 to 4 for preparing cosmetics having effects of soothing repair and anti-sensitization.
7. Use according to claim 6, characterized in that: the cosmetic is toner, lotion, essence, cream or facial mask.
8. Use according to claim 6, characterized in that: the addition amount of the composition in the cosmetic is 0.05-10% by weight percent.
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Citations (5)
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| CN108186472A (en) * | 2018-03-16 | 2018-06-22 | 江苏德和生物科技有限公司 | Lotion and preparation method thereof that a kind of antiallergic releives composition and antiallergic is releived |
| CN108272689A (en) * | 2018-03-31 | 2018-07-13 | 佛山文森特知识产权服务有限公司 | A kind of skin care compositions of Shu Min skin cares and its application |
| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN114028295A (en) * | 2021-12-10 | 2022-02-11 | 北京安德普泰医疗科技有限公司 | Antibacterial itching-relieving red-fading acne-removing repairing composition as well as preparation method and application thereof |
| CN115487112A (en) * | 2022-09-26 | 2022-12-20 | 上海臻臣化妆品有限公司 | Anti-inflammatory soothing composition for skin |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186472A (en) * | 2018-03-16 | 2018-06-22 | 江苏德和生物科技有限公司 | Lotion and preparation method thereof that a kind of antiallergic releives composition and antiallergic is releived |
| CN108272689A (en) * | 2018-03-31 | 2018-07-13 | 佛山文森特知识产权服务有限公司 | A kind of skin care compositions of Shu Min skin cares and its application |
| CN108514519A (en) * | 2018-05-11 | 2018-09-11 | 珠海伊斯佳科技股份有限公司 | A kind of skin care compositions and methods and its application method of whitening and anti-aging |
| CN114028295A (en) * | 2021-12-10 | 2022-02-11 | 北京安德普泰医疗科技有限公司 | Antibacterial itching-relieving red-fading acne-removing repairing composition as well as preparation method and application thereof |
| CN115487112A (en) * | 2022-09-26 | 2022-12-20 | 上海臻臣化妆品有限公司 | Anti-inflammatory soothing composition for skin |
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